Variety selection and planting pattern, such as single row (20 × 70 cm) and double row (20 × 35 × 70 cm), affects crops yield. This study aimed to study performance of four maize hybrid varieties grown in two planting patterns under rainfed conditions. The four varieties tested were BISI18 (PT. BISI International), NK212 (PT. Syngenta Indonesia), P21 (PT. Du Pont Indonesia) and DK 771 (Monsanto Indonesia). An experiment was conducted in a rainfed area of Gumantar, North Lombok, West Nusa Tenggara, Indonesia (8.253654 S, 116.285695 E) during the rainy season of 2018/2019. The treatments were arranged factorially using Randomized Block Design with three replications. There were 125 and 150 plants per plot for a single row and a double row planting pattern, consecutively. The results showed that no interaction between planting pattern and variety. No effect of planting pattern on plant height and leaf area at tasseling but the leaf area index (LAI) and light interception were higher in a double row pattern. The tallest crop with the highest leaf area and LAI was performed by DK771 but there was no different recorded in light interception by the crops canopy at tasseling. DK771 produced the longest ear but in term of number of kernels row per ear, BISI18 was the highest. No difference was found in yield of the four varieties and the double row planting pattern produced only 8% higher yield than the single row. All the four tested maize varieties performed well under rainfed conditions.Variety selection and planting pattern, such as single row (20 × 70 cm) and double row (20 × 35 × 70 cm), affects crops yield. This study aimed to study performance of four maize hybrid varieties grown in two planting patterns under rainfed conditions. The four varieties tested were BISI18 (PT. BISI International), NK212 (PT. Syngenta Indonesia), P21 (PT. Du Pont Indonesia) and DK 771 (Monsanto Indonesia). An experiment was conducted in a rainfed area of Gumantar, North Lombok, West Nusa Tenggara, Indonesia (8.253654 S, 116.285695 E) during the rainy season of 2018/2019. The treatments were arranged factorially using Randomized Block Design with three replications. There were 125 and 150 plants per plot for a single row and a double row planting pattern, consecutively. The results showed that no interaction between planting pattern and variety. No effect of planting pattern on plant height and leaf area at tasseling but the leaf area index (LAI) and light interception were higher in a double row pattern. ...
{"title":"Effect of two different planting patterns on performance of four maize varieties under rainfed conditions","authors":"Khaerul Ihwan, I. W. Sudika, I. Jaya","doi":"10.1063/1.5141290","DOIUrl":"https://doi.org/10.1063/1.5141290","url":null,"abstract":"Variety selection and planting pattern, such as single row (20 × 70 cm) and double row (20 × 35 × 70 cm), affects crops yield. This study aimed to study performance of four maize hybrid varieties grown in two planting patterns under rainfed conditions. The four varieties tested were BISI18 (PT. BISI International), NK212 (PT. Syngenta Indonesia), P21 (PT. Du Pont Indonesia) and DK 771 (Monsanto Indonesia). An experiment was conducted in a rainfed area of Gumantar, North Lombok, West Nusa Tenggara, Indonesia (8.253654 S, 116.285695 E) during the rainy season of 2018/2019. The treatments were arranged factorially using Randomized Block Design with three replications. There were 125 and 150 plants per plot for a single row and a double row planting pattern, consecutively. The results showed that no interaction between planting pattern and variety. No effect of planting pattern on plant height and leaf area at tasseling but the leaf area index (LAI) and light interception were higher in a double row pattern. The tallest crop with the highest leaf area and LAI was performed by DK771 but there was no different recorded in light interception by the crops canopy at tasseling. DK771 produced the longest ear but in term of number of kernels row per ear, BISI18 was the highest. No difference was found in yield of the four varieties and the double row planting pattern produced only 8% higher yield than the single row. All the four tested maize varieties performed well under rainfed conditions.Variety selection and planting pattern, such as single row (20 × 70 cm) and double row (20 × 35 × 70 cm), affects crops yield. This study aimed to study performance of four maize hybrid varieties grown in two planting patterns under rainfed conditions. The four varieties tested were BISI18 (PT. BISI International), NK212 (PT. Syngenta Indonesia), P21 (PT. Du Pont Indonesia) and DK 771 (Monsanto Indonesia). An experiment was conducted in a rainfed area of Gumantar, North Lombok, West Nusa Tenggara, Indonesia (8.253654 S, 116.285695 E) during the rainy season of 2018/2019. The treatments were arranged factorially using Randomized Block Design with three replications. There were 125 and 150 plants per plot for a single row and a double row planting pattern, consecutively. The results showed that no interaction between planting pattern and variety. No effect of planting pattern on plant height and leaf area at tasseling but the leaf area index (LAI) and light interception were higher in a double row pattern. ...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90672446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The paper deals with the assessment of the modal parameters of the washing machine heater. At first, a numerical modal analysis based on a finite element method of the heater was performed. Consequently, the experimental modal analysis of the heater was realised to verify the finite element model. As the heater’s shape and dimensions do not allow modal parameters estimation using conventional gages of acceleration, two optical approaches were applied. In both cases, the excitation was realised using Bruel&Kjaer 8206 impact hammer. In the first one, the responses of the heater were captured by Polytec PDV-100 vibrometer, which principle is based on laser Doppler vibrometry. Such a type of device allows capturing of responses in a form of velocity only in the direction of observation. To observe the responses in three mutually perpendicular directions the high-speed digital image correlation system Q-450 Dantec Dynamics was used. Mentioned correlation system uses for the full-field measurement of displacements precise digital cameras with high image resolution. As it is not adapted for modal parameters estimation, the additional software DICMAN 3D, developed at the authors’ workplace, was used. DICMAN 3D allows evaluation of a single-reference as well as a multi-reference measurement in space and for automatic estimation of natural frequencies and damping ratios comprises an advanced estimation module with several algorithms. Finally, the influence of the temperature on the modal parameters was investigated using the finite element model of the heater.The paper deals with the assessment of the modal parameters of the washing machine heater. At first, a numerical modal analysis based on a finite element method of the heater was performed. Consequently, the experimental modal analysis of the heater was realised to verify the finite element model. As the heater’s shape and dimensions do not allow modal parameters estimation using conventional gages of acceleration, two optical approaches were applied. In both cases, the excitation was realised using Bruel&Kjaer 8206 impact hammer. In the first one, the responses of the heater were captured by Polytec PDV-100 vibrometer, which principle is based on laser Doppler vibrometry. Such a type of device allows capturing of responses in a form of velocity only in the direction of observation. To observe the responses in three mutually perpendicular directions the high-speed digital image correlation system Q-450 Dantec Dynamics was used. Mentioned correlation system uses for the full-field measurement of displaceme...
{"title":"Modal analysis of the washing machine heater","authors":"M. Hagara, P. Lengvarský, R. Huňady","doi":"10.1063/1.5140864","DOIUrl":"https://doi.org/10.1063/1.5140864","url":null,"abstract":"The paper deals with the assessment of the modal parameters of the washing machine heater. At first, a numerical modal analysis based on a finite element method of the heater was performed. Consequently, the experimental modal analysis of the heater was realised to verify the finite element model. As the heater’s shape and dimensions do not allow modal parameters estimation using conventional gages of acceleration, two optical approaches were applied. In both cases, the excitation was realised using Bruel&Kjaer 8206 impact hammer. In the first one, the responses of the heater were captured by Polytec PDV-100 vibrometer, which principle is based on laser Doppler vibrometry. Such a type of device allows capturing of responses in a form of velocity only in the direction of observation. To observe the responses in three mutually perpendicular directions the high-speed digital image correlation system Q-450 Dantec Dynamics was used. Mentioned correlation system uses for the full-field measurement of displacements precise digital cameras with high image resolution. As it is not adapted for modal parameters estimation, the additional software DICMAN 3D, developed at the authors’ workplace, was used. DICMAN 3D allows evaluation of a single-reference as well as a multi-reference measurement in space and for automatic estimation of natural frequencies and damping ratios comprises an advanced estimation module with several algorithms. Finally, the influence of the temperature on the modal parameters was investigated using the finite element model of the heater.The paper deals with the assessment of the modal parameters of the washing machine heater. At first, a numerical modal analysis based on a finite element method of the heater was performed. Consequently, the experimental modal analysis of the heater was realised to verify the finite element model. As the heater’s shape and dimensions do not allow modal parameters estimation using conventional gages of acceleration, two optical approaches were applied. In both cases, the excitation was realised using Bruel&Kjaer 8206 impact hammer. In the first one, the responses of the heater were captured by Polytec PDV-100 vibrometer, which principle is based on laser Doppler vibrometry. Such a type of device allows capturing of responses in a form of velocity only in the direction of observation. To observe the responses in three mutually perpendicular directions the high-speed digital image correlation system Q-450 Dantec Dynamics was used. Mentioned correlation system uses for the full-field measurement of displaceme...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82167209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article deals with the design of a device intended for testing and research of friction characteristics of rail vehicles brake systems. For safety of running, brakes are one of the most important elements of a railway vehicle subsystems. Tasks of the subsystems are speed regulation, stopping or to keep railway vehicle motionless. Railway vehicles are used for international transportation of passengers and goods and as a consequence, large standardization of a whole railway vehicle structure has to be valid. Brake systems are included in multiple UIC and European standards. UIC 541-3 and UIC 541-4 are the most important standards for railway vehicle brake testing. In the article, there are described the process of a test of a park brake, the principle of the device operation, on which a test will be performed and a dimensional calculation of the main structural element of this device. There are presented two possible variants, on which there were performed functional and structural analyses with subsequent evaluation and a choice of the optimal variant. Results, which are obtained by means of this device, will be used for creation of new standards and rules as well as subsequent certification of brake components of rail vehicles brake systems.This article deals with the design of a device intended for testing and research of friction characteristics of rail vehicles brake systems. For safety of running, brakes are one of the most important elements of a railway vehicle subsystems. Tasks of the subsystems are speed regulation, stopping or to keep railway vehicle motionless. Railway vehicles are used for international transportation of passengers and goods and as a consequence, large standardization of a whole railway vehicle structure has to be valid. Brake systems are included in multiple UIC and European standards. UIC 541-3 and UIC 541-4 are the most important standards for railway vehicle brake testing. In the article, there are described the process of a test of a park brake, the principle of the device operation, on which a test will be performed and a dimensional calculation of the main structural element of this device. There are presented two possible variants, on which there were performed functional and structural analyses with subse...
{"title":"Design of a testing equipment for experimental research of railway brake systems","authors":"Andrej Suchánek, Pavol Kurčík, P. Šťastniak","doi":"10.1063/1.5140876","DOIUrl":"https://doi.org/10.1063/1.5140876","url":null,"abstract":"This article deals with the design of a device intended for testing and research of friction characteristics of rail vehicles brake systems. For safety of running, brakes are one of the most important elements of a railway vehicle subsystems. Tasks of the subsystems are speed regulation, stopping or to keep railway vehicle motionless. Railway vehicles are used for international transportation of passengers and goods and as a consequence, large standardization of a whole railway vehicle structure has to be valid. Brake systems are included in multiple UIC and European standards. UIC 541-3 and UIC 541-4 are the most important standards for railway vehicle brake testing. In the article, there are described the process of a test of a park brake, the principle of the device operation, on which a test will be performed and a dimensional calculation of the main structural element of this device. There are presented two possible variants, on which there were performed functional and structural analyses with subsequent evaluation and a choice of the optimal variant. Results, which are obtained by means of this device, will be used for creation of new standards and rules as well as subsequent certification of brake components of rail vehicles brake systems.This article deals with the design of a device intended for testing and research of friction characteristics of rail vehicles brake systems. For safety of running, brakes are one of the most important elements of a railway vehicle subsystems. Tasks of the subsystems are speed regulation, stopping or to keep railway vehicle motionless. Railway vehicles are used for international transportation of passengers and goods and as a consequence, large standardization of a whole railway vehicle structure has to be valid. Brake systems are included in multiple UIC and European standards. UIC 541-3 and UIC 541-4 are the most important standards for railway vehicle brake testing. In the article, there are described the process of a test of a park brake, the principle of the device operation, on which a test will be performed and a dimensional calculation of the main structural element of this device. There are presented two possible variants, on which there were performed functional and structural analyses with subse...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80780559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Astutik, Murad, G. M. D. Putra, D. A. Setiawati
This study aimed to design a Greenhouse Remote Monitoring system and to immediately evaluate the monitoring system. The Monitoring system was designed based on the NodeMCU ESP8266 microcontroller to expectedly result an Android monitoring display as the output. This research was conducted in Greenhouse at Faculty of Food and Agroindustrial Technology, University of Mataram. An experimental method using greenhouse and hydroponic system was applied to this research. The monitoring system consisted of components that functionally related to each other (NodeMCU ESP8266, Android, DHT11 (temperature and humidity sensor), BH1750 (light sensor), Total Dissolved Solid (TDS) sensors, and other components that further supported by programming language. The observed parameters were the greenhouse temperature (°C); humidity (%); light intensity (lux); and the amount of TDS (ppm). The experiment indicated that the designed monitoring system successfully displayed what the sensor had read then translated it into Android. The average percentage of the Light meter reading errors using the BH1750 sensor (shown) on Android was 2.13%; the average percentage of the hygrometer reading errors using DHT11 sensor on Android was 0.14%; the average value of the thermometer reading errors as well as DHT11 sensor on Android was 0.76%; while the average TDS reading error on Android was slightly above 5% due to increasing temperature inside the greenhouse, especially in the afternoon period.This study aimed to design a Greenhouse Remote Monitoring system and to immediately evaluate the monitoring system. The Monitoring system was designed based on the NodeMCU ESP8266 microcontroller to expectedly result an Android monitoring display as the output. This research was conducted in Greenhouse at Faculty of Food and Agroindustrial Technology, University of Mataram. An experimental method using greenhouse and hydroponic system was applied to this research. The monitoring system consisted of components that functionally related to each other (NodeMCU ESP8266, Android, DHT11 (temperature and humidity sensor), BH1750 (light sensor), Total Dissolved Solid (TDS) sensors, and other components that further supported by programming language. The observed parameters were the greenhouse temperature (°C); humidity (%); light intensity (lux); and the amount of TDS (ppm). The experiment indicated that the designed monitoring system successfully displayed what the sensor had read then translated it into Android...
{"title":"Remote monitoring systems in greenhouse based on NodeMCU ESP8266 microcontroller and Android","authors":"Y. Astutik, Murad, G. M. D. Putra, D. A. Setiawati","doi":"10.1063/1.5141286","DOIUrl":"https://doi.org/10.1063/1.5141286","url":null,"abstract":"This study aimed to design a Greenhouse Remote Monitoring system and to immediately evaluate the monitoring system. The Monitoring system was designed based on the NodeMCU ESP8266 microcontroller to expectedly result an Android monitoring display as the output. This research was conducted in Greenhouse at Faculty of Food and Agroindustrial Technology, University of Mataram. An experimental method using greenhouse and hydroponic system was applied to this research. The monitoring system consisted of components that functionally related to each other (NodeMCU ESP8266, Android, DHT11 (temperature and humidity sensor), BH1750 (light sensor), Total Dissolved Solid (TDS) sensors, and other components that further supported by programming language. The observed parameters were the greenhouse temperature (°C); humidity (%); light intensity (lux); and the amount of TDS (ppm). The experiment indicated that the designed monitoring system successfully displayed what the sensor had read then translated it into Android. The average percentage of the Light meter reading errors using the BH1750 sensor (shown) on Android was 2.13%; the average percentage of the hygrometer reading errors using DHT11 sensor on Android was 0.14%; the average value of the thermometer reading errors as well as DHT11 sensor on Android was 0.76%; while the average TDS reading error on Android was slightly above 5% due to increasing temperature inside the greenhouse, especially in the afternoon period.This study aimed to design a Greenhouse Remote Monitoring system and to immediately evaluate the monitoring system. The Monitoring system was designed based on the NodeMCU ESP8266 microcontroller to expectedly result an Android monitoring display as the output. This research was conducted in Greenhouse at Faculty of Food and Agroindustrial Technology, University of Mataram. An experimental method using greenhouse and hydroponic system was applied to this research. The monitoring system consisted of components that functionally related to each other (NodeMCU ESP8266, Android, DHT11 (temperature and humidity sensor), BH1750 (light sensor), Total Dissolved Solid (TDS) sensors, and other components that further supported by programming language. The observed parameters were the greenhouse temperature (°C); humidity (%); light intensity (lux); and the amount of TDS (ppm). The experiment indicated that the designed monitoring system successfully displayed what the sensor had read then translated it into Android...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78215193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Prasedya, N. W. R. Martyasari, Rizki Apriani, Sipti Mayshara, R. Fanani, H. Sunarpi
Green macroalgae Ulva lactuca is highly abundant in most coastal areas of Lombok, Indonesia. Previous studies have revealed Ulva contains valuable components, such as bioactive compounds, food or biofuel. In this study, we evaluated the antioxidant activity of Ulva lactuca found in different parts of Lombok Island coastal areas. The seaweeds were collected from Batu Layar (BL), Tanjung Aan (TA), and Seriwe (SW) in May 2019. Ethanol extracts of the seaweeds were determined for their antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Total phenolics were determined by colometric method with Folin-Ciocalteu reagent. Although they are the same species, all samples showed various antioxidant activities. Seaweed Ulva lactuca from TA (ULA) and Batu Layar (ULB) exhibited higher DPPH radical scavenging activity with a low IC50 522.23 ± 43 μg/mL and 534.76 ± 14 μg/mL respectively compared to Ulva lactuca from Seriwe (ULS; IC50 682.23 ± 23 μg/mL). However, positive control Ascorbic acid demonstrated significantly stronger antioxidant activity with IC50 of 67 ± 4.6 μg/mL). Furthermore, the total phenolic contents (TPC) of ULS, ULB, ULA were 2.675 ± 0.5 mgGAEg−1, 5.13 ± 0.8 mgGAEg−1, 5.33 ± 2.1 mgGAEg−1 respectively. Flavonoid contents were 19.031 ± 2.1 mgREg−1, 25.315 ± 2.6 mgREg−1, and 28. 053 ± 1.58 mgREg−1 respectively. Current data shows that different locations with different environmental conditions could result in different biological activity of the seaweeds. Green seaweed Ulva lactuca found in BL and TA with more extreme conditions could be considered as source for antioxidants and could be utilized in food and pharmaceutical industry.Green macroalgae Ulva lactuca is highly abundant in most coastal areas of Lombok, Indonesia. Previous studies have revealed Ulva contains valuable components, such as bioactive compounds, food or biofuel. In this study, we evaluated the antioxidant activity of Ulva lactuca found in different parts of Lombok Island coastal areas. The seaweeds were collected from Batu Layar (BL), Tanjung Aan (TA), and Seriwe (SW) in May 2019. Ethanol extracts of the seaweeds were determined for their antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Total phenolics were determined by colometric method with Folin-Ciocalteu reagent. Although they are the same species, all samples showed various antioxidant activities. Seaweed Ulva lactuca from TA (ULA) and Batu Layar (ULB) exhibited higher DPPH radical scavenging activity with a low IC50 522.23 ± 43 μg/mL and 534.76 ± 14 μg/mL respectively compared to Ulva lactuca from Seriwe (ULS; IC50 682.23 ± 23 μg/mL). However, positive control Asco...
{"title":"Antioxidant activity of Ulva lactuca L. from different coastal locations of Lombok Island, Indonesia","authors":"E. Prasedya, N. W. R. Martyasari, Rizki Apriani, Sipti Mayshara, R. Fanani, H. Sunarpi","doi":"10.1063/1.5141281","DOIUrl":"https://doi.org/10.1063/1.5141281","url":null,"abstract":"Green macroalgae Ulva lactuca is highly abundant in most coastal areas of Lombok, Indonesia. Previous studies have revealed Ulva contains valuable components, such as bioactive compounds, food or biofuel. In this study, we evaluated the antioxidant activity of Ulva lactuca found in different parts of Lombok Island coastal areas. The seaweeds were collected from Batu Layar (BL), Tanjung Aan (TA), and Seriwe (SW) in May 2019. Ethanol extracts of the seaweeds were determined for their antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Total phenolics were determined by colometric method with Folin-Ciocalteu reagent. Although they are the same species, all samples showed various antioxidant activities. Seaweed Ulva lactuca from TA (ULA) and Batu Layar (ULB) exhibited higher DPPH radical scavenging activity with a low IC50 522.23 ± 43 μg/mL and 534.76 ± 14 μg/mL respectively compared to Ulva lactuca from Seriwe (ULS; IC50 682.23 ± 23 μg/mL). However, positive control Ascorbic acid demonstrated significantly stronger antioxidant activity with IC50 of 67 ± 4.6 μg/mL). Furthermore, the total phenolic contents (TPC) of ULS, ULB, ULA were 2.675 ± 0.5 mgGAEg−1, 5.13 ± 0.8 mgGAEg−1, 5.33 ± 2.1 mgGAEg−1 respectively. Flavonoid contents were 19.031 ± 2.1 mgREg−1, 25.315 ± 2.6 mgREg−1, and 28. 053 ± 1.58 mgREg−1 respectively. Current data shows that different locations with different environmental conditions could result in different biological activity of the seaweeds. Green seaweed Ulva lactuca found in BL and TA with more extreme conditions could be considered as source for antioxidants and could be utilized in food and pharmaceutical industry.Green macroalgae Ulva lactuca is highly abundant in most coastal areas of Lombok, Indonesia. Previous studies have revealed Ulva contains valuable components, such as bioactive compounds, food or biofuel. In this study, we evaluated the antioxidant activity of Ulva lactuca found in different parts of Lombok Island coastal areas. The seaweeds were collected from Batu Layar (BL), Tanjung Aan (TA), and Seriwe (SW) in May 2019. Ethanol extracts of the seaweeds were determined for their antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Total phenolics were determined by colometric method with Folin-Ciocalteu reagent. Although they are the same species, all samples showed various antioxidant activities. Seaweed Ulva lactuca from TA (ULA) and Batu Layar (ULB) exhibited higher DPPH radical scavenging activity with a low IC50 522.23 ± 43 μg/mL and 534.76 ± 14 μg/mL respectively compared to Ulva lactuca from Seriwe (ULS; IC50 682.23 ± 23 μg/mL). However, positive control Asco...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84708636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nurhaerani, Wayan Wariata, D. Kisworo, S. Depamede
The main objective of this study was to purify polyclonal antibodies against pork extract as raw material for developing diagnostic kits for halal food made from animal products. Antibodies were obtained from rabbit serum which was immunized with raw pork extract, and which had been heated at 70° C, 80° C and 100° C. The purification process was carried out in two stages namely using 33% ammonium sulfate and continued with Protein A column affinity. The antibody purification results were analyzed using SDS-PAGE, while the antibody activity was tested using the western blot method on raw meat extracts and on those heated at 70° C, 80° C, and 100° C. The results of this study showed that antibodies purified with a combination of ammonium sulfate and Protein A column affinity produced relative purity of antibodies up to 97.6 per cent compared to those purified using ammonium sulfate alone. Western blot analysis showed that the purified antibodies were able to detect raw as well as heated pork extracts up to 70° C at a total protein content of 20 micro grams of meat extract. Antibodies obtained from immunizations using meat extracts that have been heated at 70° C gave the same potential as antibodies from rabbits immunized using raw meat extract, i.e. can only detect antigens of raw meat and antigens of pork heated at 70° C. From the results of this study it can be concluded that purification of antibodies using a combination of ammonium sulfate and Protein A column is effective in producing polyclonal antibodies with relatively high purity levels. In the future, further research needs to be carried out on the development of immunodiagnostics using polyclonal antibodies purified from animals immunized with pork extract that has been heated at a temperature of 70° C or more.The main objective of this study was to purify polyclonal antibodies against pork extract as raw material for developing diagnostic kits for halal food made from animal products. Antibodies were obtained from rabbit serum which was immunized with raw pork extract, and which had been heated at 70° C, 80° C and 100° C. The purification process was carried out in two stages namely using 33% ammonium sulfate and continued with Protein A column affinity. The antibody purification results were analyzed using SDS-PAGE, while the antibody activity was tested using the western blot method on raw meat extracts and on those heated at 70° C, 80° C, and 100° C. The results of this study showed that antibodies purified with a combination of ammonium sulfate and Protein A column affinity produced relative purity of antibodies up to 97.6 per cent compared to those purified using ammonium sulfate alone. Western blot analysis showed that the purified antibodies were able to detect raw as well as heated pork extracts up to ...
{"title":"Purification of polyclonal antibody against pork extracts antigens using protein A column as material for developing halal food detection kit","authors":"Nurhaerani, Wayan Wariata, D. Kisworo, S. Depamede","doi":"10.1063/1.5141328","DOIUrl":"https://doi.org/10.1063/1.5141328","url":null,"abstract":"The main objective of this study was to purify polyclonal antibodies against pork extract as raw material for developing diagnostic kits for halal food made from animal products. Antibodies were obtained from rabbit serum which was immunized with raw pork extract, and which had been heated at 70° C, 80° C and 100° C. The purification process was carried out in two stages namely using 33% ammonium sulfate and continued with Protein A column affinity. The antibody purification results were analyzed using SDS-PAGE, while the antibody activity was tested using the western blot method on raw meat extracts and on those heated at 70° C, 80° C, and 100° C. The results of this study showed that antibodies purified with a combination of ammonium sulfate and Protein A column affinity produced relative purity of antibodies up to 97.6 per cent compared to those purified using ammonium sulfate alone. Western blot analysis showed that the purified antibodies were able to detect raw as well as heated pork extracts up to 70° C at a total protein content of 20 micro grams of meat extract. Antibodies obtained from immunizations using meat extracts that have been heated at 70° C gave the same potential as antibodies from rabbits immunized using raw meat extract, i.e. can only detect antigens of raw meat and antigens of pork heated at 70° C. From the results of this study it can be concluded that purification of antibodies using a combination of ammonium sulfate and Protein A column is effective in producing polyclonal antibodies with relatively high purity levels. In the future, further research needs to be carried out on the development of immunodiagnostics using polyclonal antibodies purified from animals immunized with pork extract that has been heated at a temperature of 70° C or more.The main objective of this study was to purify polyclonal antibodies against pork extract as raw material for developing diagnostic kits for halal food made from animal products. Antibodies were obtained from rabbit serum which was immunized with raw pork extract, and which had been heated at 70° C, 80° C and 100° C. The purification process was carried out in two stages namely using 33% ammonium sulfate and continued with Protein A column affinity. The antibody purification results were analyzed using SDS-PAGE, while the antibody activity was tested using the western blot method on raw meat extracts and on those heated at 70° C, 80° C, and 100° C. The results of this study showed that antibodies purified with a combination of ammonium sulfate and Protein A column affinity produced relative purity of antibodies up to 97.6 per cent compared to those purified using ammonium sulfate alone. Western blot analysis showed that the purified antibodies were able to detect raw as well as heated pork extracts up to ...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87288297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sunarpi, Nurul Noviandi Nahdia Putri, Y. Ambana, A. Nikmatullah, Suparman, E. Prasedya
Brown algae is known to produce several phytohormons. Therefore, it could be develop as an important biostimulant for growth of agriculture and horticultural plants. Action of biostimulant depends on optimal time of application. This article report growth and yield of rice plant supplied with liquid extract of several species of brown algae, either during vegetative or generative growth. The treathment influenced variably growth and yield of rice plants. Liquid extract of Sargassum crassifolium and Sargassum polycistum did not influenced growth and yield of rice plants, either supplied in combination with Turbinaria murayana extract or not during generative growth. However, application Sargassum cristaefolium and mixed extract consistenly effected growth and yield of rice plants. Application of Sargassum cristaefolium extract increased shoot dry weight, appoximately 94,70% higher than of control. In contrast, when this plants sprayed with Turbinaria murayana extract during generative growth reduced shoot dry weight, approximately 25% than those sprayed only with Sargassum cristaefolium during vegetative growth. Since the plants supplied with Sargassum cristaefolium during vegetative growth in combination with Turbinaria murayana during generative growth, induced grain weight of rice plants, this suggest that application of Turbinaria murayana extract induced remonbilization of macromolecules such carbohydrate and protein from leave to the grain of rice.Brown algae is known to produce several phytohormons. Therefore, it could be develop as an important biostimulant for growth of agriculture and horticultural plants. Action of biostimulant depends on optimal time of application. This article report growth and yield of rice plant supplied with liquid extract of several species of brown algae, either during vegetative or generative growth. The treathment influenced variably growth and yield of rice plants. Liquid extract of Sargassum crassifolium and Sargassum polycistum did not influenced growth and yield of rice plants, either supplied in combination with Turbinaria murayana extract or not during generative growth. However, application Sargassum cristaefolium and mixed extract consistenly effected growth and yield of rice plants. Application of Sargassum cristaefolium extract increased shoot dry weight, appoximately 94,70% higher than of control. In contrast, when this plants sprayed with Turbinaria murayana extract during generative growth reduced shoot ...
{"title":"The evidence suggesting that Turbinaria murayana extract induce remobilization of macromolecule from leave to grain of rice plants","authors":"H. Sunarpi, Nurul Noviandi Nahdia Putri, Y. Ambana, A. Nikmatullah, Suparman, E. Prasedya","doi":"10.1063/1.5141324","DOIUrl":"https://doi.org/10.1063/1.5141324","url":null,"abstract":"Brown algae is known to produce several phytohormons. Therefore, it could be develop as an important biostimulant for growth of agriculture and horticultural plants. Action of biostimulant depends on optimal time of application. This article report growth and yield of rice plant supplied with liquid extract of several species of brown algae, either during vegetative or generative growth. The treathment influenced variably growth and yield of rice plants. Liquid extract of Sargassum crassifolium and Sargassum polycistum did not influenced growth and yield of rice plants, either supplied in combination with Turbinaria murayana extract or not during generative growth. However, application Sargassum cristaefolium and mixed extract consistenly effected growth and yield of rice plants. Application of Sargassum cristaefolium extract increased shoot dry weight, appoximately 94,70% higher than of control. In contrast, when this plants sprayed with Turbinaria murayana extract during generative growth reduced shoot dry weight, approximately 25% than those sprayed only with Sargassum cristaefolium during vegetative growth. Since the plants supplied with Sargassum cristaefolium during vegetative growth in combination with Turbinaria murayana during generative growth, induced grain weight of rice plants, this suggest that application of Turbinaria murayana extract induced remonbilization of macromolecules such carbohydrate and protein from leave to the grain of rice.Brown algae is known to produce several phytohormons. Therefore, it could be develop as an important biostimulant for growth of agriculture and horticultural plants. Action of biostimulant depends on optimal time of application. This article report growth and yield of rice plant supplied with liquid extract of several species of brown algae, either during vegetative or generative growth. The treathment influenced variably growth and yield of rice plants. Liquid extract of Sargassum crassifolium and Sargassum polycistum did not influenced growth and yield of rice plants, either supplied in combination with Turbinaria murayana extract or not during generative growth. However, application Sargassum cristaefolium and mixed extract consistenly effected growth and yield of rice plants. Application of Sargassum cristaefolium extract increased shoot dry weight, appoximately 94,70% higher than of control. In contrast, when this plants sprayed with Turbinaria murayana extract during generative growth reduced shoot ...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82977782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yellow leaf curl virus causes pepper yellow leaf curl diseases that caused by Geminiviruses (Begomovirus) became epidemic in Indonesia on 2000. This disease is widespread at several chili pepper production centers in Java, Sumatera, Bali, Kalimantan, and Sulawesi. There is no report about existence of it in Lombok island, West Nusa Tenggara Province, one of chili pepper production centre in Indonesia. The aim of this research is to identify Begomovirus which infect chili pepper with molecular approach and to find out the variabilities genetic of Begomovirus from Lombok island, Central Jawa and DIY with DNA fingerprint pattern based on Polymerase Chain Reaction (PCR) and Reaction Fragment Length Polymorphisme (RFLP). This research conducted with this following step: field observation of yellow leaf curl symptoms, DNA extraction, amplification the coat protein with spesific primer PYLCV, and RFLP analyses. Field result that conducted in three regency of Lombok island (East Lombok, West Lombok and Central Lombok) shown that infection symptoms in chili pepper such as various yellow-green spot in leaves, leaf curl, become small and wrapped on top, and in plant that have severe infection the plant become stunted. Results of molecular identification using PCR in sample plant aredetected the Begomovirus existence with amplification products sized 840 bp. The analysis results of PCR-RFLP using restriction enzymes HaeIII have shown the genetic variability between isolate from Lombok island, Centre of Java, and DIY. The result of this research has proved the ability of PYLCV primer in PCR to detect Begomovirus obtained from different places. This research proved that the universal primer ability in PCR for Begomovirus detection from different area and as the first report there are infections of Pepper Yellow Leaf Curl Virus in Lombok island NTB.Yellow leaf curl virus causes pepper yellow leaf curl diseases that caused by Geminiviruses (Begomovirus) became epidemic in Indonesia on 2000. This disease is widespread at several chili pepper production centers in Java, Sumatera, Bali, Kalimantan, and Sulawesi. There is no report about existence of it in Lombok island, West Nusa Tenggara Province, one of chili pepper production centre in Indonesia. The aim of this research is to identify Begomovirus which infect chili pepper with molecular approach and to find out the variabilities genetic of Begomovirus from Lombok island, Central Jawa and DIY with DNA fingerprint pattern based on Polymerase Chain Reaction (PCR) and Reaction Fragment Length Polymorphisme (RFLP). This research conducted with this following step: field observation of yellow leaf curl symptoms, DNA extraction, amplification the coat protein with spesific primer PYLCV, and RFLP analyses. Field result that conducted in three regency of Lombok island (East Lombok, West Lombok and Central Lo...
{"title":"Identification of virus causing the yellow leaf curl diseases on chili pepper in Lombok Island by PCR-RFLP technique","authors":"M. Windarningsih","doi":"10.1063/1.5141297","DOIUrl":"https://doi.org/10.1063/1.5141297","url":null,"abstract":"Yellow leaf curl virus causes pepper yellow leaf curl diseases that caused by Geminiviruses (Begomovirus) became epidemic in Indonesia on 2000. This disease is widespread at several chili pepper production centers in Java, Sumatera, Bali, Kalimantan, and Sulawesi. There is no report about existence of it in Lombok island, West Nusa Tenggara Province, one of chili pepper production centre in Indonesia. The aim of this research is to identify Begomovirus which infect chili pepper with molecular approach and to find out the variabilities genetic of Begomovirus from Lombok island, Central Jawa and DIY with DNA fingerprint pattern based on Polymerase Chain Reaction (PCR) and Reaction Fragment Length Polymorphisme (RFLP). This research conducted with this following step: field observation of yellow leaf curl symptoms, DNA extraction, amplification the coat protein with spesific primer PYLCV, and RFLP analyses. Field result that conducted in three regency of Lombok island (East Lombok, West Lombok and Central Lombok) shown that infection symptoms in chili pepper such as various yellow-green spot in leaves, leaf curl, become small and wrapped on top, and in plant that have severe infection the plant become stunted. Results of molecular identification using PCR in sample plant aredetected the Begomovirus existence with amplification products sized 840 bp. The analysis results of PCR-RFLP using restriction enzymes HaeIII have shown the genetic variability between isolate from Lombok island, Centre of Java, and DIY. The result of this research has proved the ability of PYLCV primer in PCR to detect Begomovirus obtained from different places. This research proved that the universal primer ability in PCR for Begomovirus detection from different area and as the first report there are infections of Pepper Yellow Leaf Curl Virus in Lombok island NTB.Yellow leaf curl virus causes pepper yellow leaf curl diseases that caused by Geminiviruses (Begomovirus) became epidemic in Indonesia on 2000. This disease is widespread at several chili pepper production centers in Java, Sumatera, Bali, Kalimantan, and Sulawesi. There is no report about existence of it in Lombok island, West Nusa Tenggara Province, one of chili pepper production centre in Indonesia. The aim of this research is to identify Begomovirus which infect chili pepper with molecular approach and to find out the variabilities genetic of Begomovirus from Lombok island, Central Jawa and DIY with DNA fingerprint pattern based on Polymerase Chain Reaction (PCR) and Reaction Fragment Length Polymorphisme (RFLP). This research conducted with this following step: field observation of yellow leaf curl symptoms, DNA extraction, amplification the coat protein with spesific primer PYLCV, and RFLP analyses. Field result that conducted in three regency of Lombok island (East Lombok, West Lombok and Central Lo...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88605603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sunarpi, S. Pebriani, Y. Ambana, Fadhillah Eka Putri, A. Nikmatullah, M. Ghazali, R. Kurnianingsih, E. Prasedya
High dose application of inorganic fertilizer in agriculture creats several problems, such as high cost production, reduce farmers income and solid fertility, and harm our environment. Therefore, it is needed to understand a fertilizer which is cheap, abundant raw material and adaptive to environment. Many author reported that macroalga could be used as raw materials for developing organic fertilizer as it contains phytohormones and essential alements stimulating growth and production of plants. This article reports the effect of anorganic fertilizers and brown alga solid extract on growth and yield of rice plants. The experiments consisted of two factors, dose of anorganic fertilizer (0,25,50, and 100%), and brown alga solid extract (P0 and P1). Since there was eight combinations, and each combination was repeated three times, then the experiment consisted of 24 experimental pot. The results shown application of 25% anorganic fertilizer in combination with solid axtract increased growth and yield of rice plants on the similar value when the rice plants supplied with 50% or 100% dose of anorganic fertilizer. This suggests that the application of 25% anorganic fertilizer in combination with solid extract could increase efficiency in using organic fertilizer into 75%.High dose application of inorganic fertilizer in agriculture creats several problems, such as high cost production, reduce farmers income and solid fertility, and harm our environment. Therefore, it is needed to understand a fertilizer which is cheap, abundant raw material and adaptive to environment. Many author reported that macroalga could be used as raw materials for developing organic fertilizer as it contains phytohormones and essential alements stimulating growth and production of plants. This article reports the effect of anorganic fertilizers and brown alga solid extract on growth and yield of rice plants. The experiments consisted of two factors, dose of anorganic fertilizer (0,25,50, and 100%), and brown alga solid extract (P0 and P1). Since there was eight combinations, and each combination was repeated three times, then the experiment consisted of 24 experimental pot. The results shown application of 25% anorganic fertilizer in combination with solid axtract increased growth and yield of rice...
{"title":"Effect of inorganic fertilizer and brown alga solid ectract on growth and yield of rice plants","authors":"H. Sunarpi, S. Pebriani, Y. Ambana, Fadhillah Eka Putri, A. Nikmatullah, M. Ghazali, R. Kurnianingsih, E. Prasedya","doi":"10.1063/1.5141320","DOIUrl":"https://doi.org/10.1063/1.5141320","url":null,"abstract":"High dose application of inorganic fertilizer in agriculture creats several problems, such as high cost production, reduce farmers income and solid fertility, and harm our environment. Therefore, it is needed to understand a fertilizer which is cheap, abundant raw material and adaptive to environment. Many author reported that macroalga could be used as raw materials for developing organic fertilizer as it contains phytohormones and essential alements stimulating growth and production of plants. This article reports the effect of anorganic fertilizers and brown alga solid extract on growth and yield of rice plants. The experiments consisted of two factors, dose of anorganic fertilizer (0,25,50, and 100%), and brown alga solid extract (P0 and P1). Since there was eight combinations, and each combination was repeated three times, then the experiment consisted of 24 experimental pot. The results shown application of 25% anorganic fertilizer in combination with solid axtract increased growth and yield of rice plants on the similar value when the rice plants supplied with 50% or 100% dose of anorganic fertilizer. This suggests that the application of 25% anorganic fertilizer in combination with solid extract could increase efficiency in using organic fertilizer into 75%.High dose application of inorganic fertilizer in agriculture creats several problems, such as high cost production, reduce farmers income and solid fertility, and harm our environment. Therefore, it is needed to understand a fertilizer which is cheap, abundant raw material and adaptive to environment. Many author reported that macroalga could be used as raw materials for developing organic fertilizer as it contains phytohormones and essential alements stimulating growth and production of plants. This article reports the effect of anorganic fertilizers and brown alga solid extract on growth and yield of rice plants. The experiments consisted of two factors, dose of anorganic fertilizer (0,25,50, and 100%), and brown alga solid extract (P0 and P1). Since there was eight combinations, and each combination was repeated three times, then the experiment consisted of 24 experimental pot. The results shown application of 25% anorganic fertilizer in combination with solid axtract increased growth and yield of rice...","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77648386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research was aimed to determine the effects of perilla seeds (Perilla frutescens L.) levels in the diet on the percentage of carcass and non carcass, chemical quality and levels α-linolenic acid (ALA) of meat ducks. The research design was one way Completely Randomized Design with 3 treatments, 6 replications and each replication consisted of 6 head ducks. The treatment included; P0 : (100% basal feed); P1 : (97.5% basal feed + 2.5% perilla seed) and P2 : (95% basal feed + 5% perilla seed). The carcassing process was carried out when the age of the local ducks reaches eight weeks by slaughtering 2 head ducks for each replication. Materials used 36 samples of breast duck meat were observed. The experiment was conducted for 60 days. The data were analyzed with one way analysis of variance (ANOVA). If there was significant differences among treatments, Duncan’s multiple range test (DMRT) were applied. The result shows the carcass weight and non carcass percentage were not significant (P>0.05), but breast percentage and liver percentage were significant (P<0.05). The perilla seed supplementation had significant effect (P<0.05) on increased levels of protein, cholesterol and α-linolenic acid and also had a very significant effect (P<0.01) on decrease level of meat fats. The conclusion of this research is the enriched of perilla seed at the level of 5% in basal feed does not increased the carcass weight, percentage carcass and non carcass, but increased percentage breast and liver. The quality of protein and omega-3 fatty acids and reduce fat levels, but had not been able to reduce cholesterol levels in local duck meat.
本试验旨在研究饲粮中紫苏籽(perilla frutescens L.)水平对肉鸭胴体和非胴体率、化学品质和α-亚麻酸(ALA)水平的影响。试验设计为单路完全随机设计，3个处理，6个重复，每个重复6头鸭。治疗方法包括;P0:(100%基础饲料);P1:(97.5%基础饲料+ 2.5%紫苏籽)和P2:(95%基础饲料+ 5%紫苏籽)。屠宰过程在当地鸭龄达到8周龄时进行，每次复制屠宰2头鸭。材料采用36份鸭胸肉样品进行观察。试验期60 d。采用单因素方差分析(ANOVA)对数据进行分析。如处理间差异显著，则采用Duncan 's multiple range test (DMRT)。结果表明:胴体重和非胴体率差异不显著(P>0.05)，胸率和肝率差异显著(P<0.05)。添加紫苏籽对提高蛋白质、胆固醇和α-亚麻酸水平有极显著影响(P<0.05)，对降低肉脂肪水平有极显著影响(P<0.01)。本研究的结论是，在基础饲料中添加5%水平的紫苏籽并没有提高胴体重、胴体率和非胴体率，但提高了胸肉率和肝脏率。优质的蛋白质和欧米伽-3脂肪酸能降低脂肪水平，但未能降低当地鸭肉中的胆固醇水平。
{"title":"The effect of enriched perilla seed (Perilla frutescens L.) in the diets on percentage of carcass and non-carcass, chemical quality, and levels of α-linoleic acid (ALA) of meat ducks","authors":"R. F. Hadi, Sudiyono, S. Jannah, W. Indriyani","doi":"10.1063/1.5141306","DOIUrl":"https://doi.org/10.1063/1.5141306","url":null,"abstract":"This research was aimed to determine the effects of perilla seeds (Perilla frutescens L.) levels in the diet on the percentage of carcass and non carcass, chemical quality and levels α-linolenic acid (ALA) of meat ducks. The research design was one way Completely Randomized Design with 3 treatments, 6 replications and each replication consisted of 6 head ducks. The treatment included; P0 : (100% basal feed); P1 : (97.5% basal feed + 2.5% perilla seed) and P2 : (95% basal feed + 5% perilla seed). The carcassing process was carried out when the age of the local ducks reaches eight weeks by slaughtering 2 head ducks for each replication. Materials used 36 samples of breast duck meat were observed. The experiment was conducted for 60 days. The data were analyzed with one way analysis of variance (ANOVA). If there was significant differences among treatments, Duncan’s multiple range test (DMRT) were applied. The result shows the carcass weight and non carcass percentage were not significant (P>0.05), but breast percentage and liver percentage were significant (P<0.05). The perilla seed supplementation had significant effect (P<0.05) on increased levels of protein, cholesterol and α-linolenic acid and also had a very significant effect (P<0.01) on decrease level of meat fats. The conclusion of this research is the enriched of perilla seed at the level of 5% in basal feed does not increased the carcass weight, percentage carcass and non carcass, but increased percentage breast and liver. The quality of protein and omega-3 fatty acids and reduce fat levels, but had not been able to reduce cholesterol levels in local duck meat.","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90603610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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