Pub Date : 2022-01-08DOI: 10.21285/2227-2925-2021-11-4-590-602
Наталья Борисовна Еремеева, Надежда Викторовна Макарова, N. Eremeeva, Nadezhda V. Makarova
Plant extracts rich in polyphenols can be used in the food industry as natural preservatives, extending the shelf life of prepared and semi-finished foods without chemical preservatives. In this paper, we investigate the polyphenolic composition, antioxidant activity and antibacterial properties of herbal extracts as part of food systems. The research objects were knot grass (Polýgonum aviculáre), marjoram (Oríganum), bur beggar-ticks (Bídenstripartíta), thyme (Thymus), whortleberry leaves (Vaccínium ida vítis), calendula (Calendula), sage (Salvia), chamomile flowers (Matricāriachamomīlla), eucalyptus (Eucalýptus) and bearberry (Arctostáphylosúva-úrsi). We determined the total content of phenolic compounds and flavonoids; the antioxidant activity by DPPH and FRAP methods; variations in the bacterial сontamination of animal raw materials over total bacterial count (TBC), coliform bacteria, yeast/fungi, salmonella and staphylococcus. Extracts of sage (1138±57 mg GA/100 g and 537±25 mg C/100 g), eucalyptus (1073±49 mg GA/100 g and 412±20 mg C/100 g), chamomile flowers (1002±36 mg GA/100 g and 493±22 mg C/100 g) and marjoram (1015±42 mg GA/100 g and 458±21 mg C/100 g) contain the largest amount of biologically active substances (phenols and flavonoids, respectively). Sage, eucalyptus and chamomile extracts demonstrate the highest antioxidant activity among the studied samples. Most of the studied extracts exhibit little or no effect on the organoleptic properties of finished products. In addition, chamomile flower, sage and eucalyptus extracts suppress the growth of pathogenic microorganisms in foods under experimental conditions. The microflora growth is significantly reduced when treating animal raw materials with calendula flowers, marjoram and thyme extracts. Extracts of sage, chamomile flowers, calendula flowers, marjoram and thyme can be recommended as components of food raw materials.
{"title":"Antioxidant properties of medicinal plants and their effect on microbial spoilage of semi-finished meat, poultry and fish","authors":"Наталья Борисовна Еремеева, Надежда Викторовна Макарова, N. Eremeeva, Nadezhda V. Makarova","doi":"10.21285/2227-2925-2021-11-4-590-602","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-590-602","url":null,"abstract":"Plant extracts rich in polyphenols can be used in the food industry as natural preservatives, extending the shelf life of prepared and semi-finished foods without chemical preservatives. In this paper, we investigate the polyphenolic composition, antioxidant activity and antibacterial properties of herbal extracts as part of food systems. The research objects were knot grass (Polýgonum aviculáre), marjoram (Oríganum), bur beggar-ticks (Bídenstripartíta), thyme (Thymus), whortleberry leaves (Vaccínium ida vítis), calendula (Calendula), sage (Salvia), chamomile flowers (Matricāriachamomīlla), eucalyptus (Eucalýptus) and bearberry (Arctostáphylosúva-úrsi). We determined the total content of phenolic compounds and flavonoids; the antioxidant activity by DPPH and FRAP methods; variations in the bacterial сontamination of animal raw materials over total bacterial count (TBC), coliform bacteria, yeast/fungi, salmonella and staphylococcus. Extracts of sage (1138±57 mg GA/100 g and 537±25 mg C/100 g), eucalyptus (1073±49 mg GA/100 g and 412±20 mg C/100 g), chamomile flowers (1002±36 mg GA/100 g and 493±22 mg C/100 g) and marjoram (1015±42 mg GA/100 g and 458±21 mg C/100 g) contain the largest amount of biologically active substances (phenols and flavonoids, respectively). Sage, eucalyptus and chamomile extracts demonstrate the highest antioxidant activity among the studied samples. Most of the studied extracts exhibit little or no effect on the organoleptic properties of finished products. In addition, chamomile flower, sage and eucalyptus extracts suppress the growth of pathogenic microorganisms in foods under experimental conditions. The microflora growth is significantly reduced when treating animal raw materials with calendula flowers, marjoram and thyme extracts. Extracts of sage, chamomile flowers, calendula flowers, marjoram and thyme can be recommended as components of food raw materials.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75354863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-08DOI: 10.21285/2227-2925-2021-11-4-581-589
V. Chkhenkeli
Given the spread of bacterial and viral diseases in young farm animals, the use of interferons and drugs to stimulate their biosynthesis has gained relevance. In a previous study, we examined the effect of a veterinary drug Trametin produced on the basis of Trametes pubescens (Shumach.: Fr.) Pilat. on the biosynthesis of interferons in the blood of mice. The present work is aimed at studying the biosynthesis dynamics of α- and γ-interferons when using Trametin and studying its prophylactic activity in calves. It is shown that a single oral administration of Trametin in doses ranging from 15 to 60 mg/kg causes a dose-dependent induction and production of γ-interferon in the blood of mice, whose maximum content reaches 1337.0±93.0 pg/mL at 48 h after administering a dose of 30 mg/kg. With a Trametin dose increase from 15 to 30 mg/kg, the level of α-interferon production rises to 1388.0±84.0 pg/mL at 48 h after administration. At a Cycloferon dose of 4.5 mg/kg, the production level of α-interferon and γ-interferon amounts to 1455.47±84.2 and 1447.0±90.0 pg/mL, respectively. The immunostimulatory properties of Trametin are confirmed by a scientific and economic experiment conducted using immunocompromised calves. In our studies, an immunological test of calf blood performed prior to and following the administration of Trimetin and Cycloferon constitutes criteria for the prophylactic activity of these drugs. The prophylactic efficacy of Trametin is confirmed by an increase in phagocytic activity by 10.5%, phagocytic index by 61.8%, and phagocytic number by 52.8%. After Trametin administration, the bactericidal activity of the serum increases by 60%. Cycloferon exhibits a similar immunostimulatory effect. Nonspecific prophylaxis using Trametin is shown to reduce the incidence of bacterial and viral respiratory diseases in young calves and generally improve their immunity.
{"title":"Veterinary drug Trametin obtained on the basis of Trametes pubescens xylotroph fungi: its effect on the biosynthesis of interferons and its prophylactic activity against calf respiratory diseases","authors":"V. Chkhenkeli","doi":"10.21285/2227-2925-2021-11-4-581-589","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-581-589","url":null,"abstract":"Given the spread of bacterial and viral diseases in young farm animals, the use of interferons and drugs to stimulate their biosynthesis has gained relevance. In a previous study, we examined the effect of a veterinary drug Trametin produced on the basis of Trametes pubescens (Shumach.: Fr.) Pilat. on the biosynthesis of interferons in the blood of mice. The present work is aimed at studying the biosynthesis dynamics of α- and γ-interferons when using Trametin and studying its prophylactic activity in calves. It is shown that a single oral administration of Trametin in doses ranging from 15 to 60 mg/kg causes a dose-dependent induction and production of γ-interferon in the blood of mice, whose maximum content reaches 1337.0±93.0 pg/mL at 48 h after administering a dose of 30 mg/kg. With a Trametin dose increase from 15 to 30 mg/kg, the level of α-interferon production rises to 1388.0±84.0 pg/mL at 48 h after administration. At a Cycloferon dose of 4.5 mg/kg, the production level of α-interferon and γ-interferon amounts to 1455.47±84.2 and 1447.0±90.0 pg/mL, respectively. The immunostimulatory properties of Trametin are confirmed by a scientific and economic experiment conducted using immunocompromised calves. In our studies, an immunological test of calf blood performed prior to and following the administration of Trimetin and Cycloferon constitutes criteria for the prophylactic activity of these drugs. The prophylactic efficacy of Trametin is confirmed by an increase in phagocytic activity by 10.5%, phagocytic index by 61.8%, and phagocytic number by 52.8%. After Trametin administration, the bactericidal activity of the serum increases by 60%. Cycloferon exhibits a similar immunostimulatory effect. Nonspecific prophylaxis using Trametin is shown to reduce the incidence of bacterial and viral respiratory diseases in young calves and generally improve their immunity.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89666769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-08DOI: 10.21285/2227-2925-2021-11-4-617-626
A. Kobelev, S. V. Klement’ev, A. Sirotkin
We examine the agglutinating ability of five compounds, namely, A1, A2, A3, A4 and BS1, isolated from activated sludge on selective media typical of a number of dominant microbial cultures that contribute to the formation of microbial aggregates. The morphological properties of the isolates and their lectin activity, as well as the physiological and biochemical properties of individual isolates were studied; microorganisms in their composition were identified. We assessed the capacity of the isolates under study to synthesize an exopolysaccharide matrix, as well as the sedimentation of activated sludge under the action of the native solution and culture liquid of the BS1 isolate. Based on their capacity to agglutinate, the BS1 and A2 isolates were selected for further research as producers of extracellular lectins and objects of agglutination, respectively. The biophysiochemical properties and molecular-genetic identification of the BS1 isolate allowed the degree of identity with r. Bacillus to be defined (96.19%); for the A2 isolate, 92.93% identity with p. Shigella and p. Escherichia was determined. To assess the capacity to synthesize a biofilm matrix, the BS1 and A2 isolates were cultivated on an agar nutrient solution using Congo Red dye. According to the obtained results, the isolates are capable of synthesizing an exopolysaccharide matrix, the main component of bacterial biofilms. The research results on the sedimentation of activated sludge induced by the native solution and culture liquid of BS1 showed the following. The sedimentation rate of activated sludge increased significantly at the beginning of the process upon adding a BS1 cell suspension, while the introduction of the native solution of BS1 intensified the process following 5 minutes of contact. The obtained experimental data suggest that the media containing extracellular bacterial lectins can be effectively used as a coagulant (flocculant) for the sedimentation of activated sludge.
{"title":"Agglutination processes of activated sludge cultures induced by extracellular lectins","authors":"A. Kobelev, S. V. Klement’ev, A. Sirotkin","doi":"10.21285/2227-2925-2021-11-4-617-626","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-617-626","url":null,"abstract":"We examine the agglutinating ability of five compounds, namely, A1, A2, A3, A4 and BS1, isolated from activated sludge on selective media typical of a number of dominant microbial cultures that contribute to the formation of microbial aggregates. The morphological properties of the isolates and their lectin activity, as well as the physiological and biochemical properties of individual isolates were studied; microorganisms in their composition were identified. We assessed the capacity of the isolates under study to synthesize an exopolysaccharide matrix, as well as the sedimentation of activated sludge under the action of the native solution and culture liquid of the BS1 isolate. Based on their capacity to agglutinate, the BS1 and A2 isolates were selected for further research as producers of extracellular lectins and objects of agglutination, respectively. The biophysiochemical properties and molecular-genetic identification of the BS1 isolate allowed the degree of identity with r. Bacillus to be defined (96.19%); for the A2 isolate, 92.93% identity with p. Shigella and p. Escherichia was determined. To assess the capacity to synthesize a biofilm matrix, the BS1 and A2 isolates were cultivated on an agar nutrient solution using Congo Red dye. According to the obtained results, the isolates are capable of synthesizing an exopolysaccharide matrix, the main component of bacterial biofilms. The research results on the sedimentation of activated sludge induced by the native solution and culture liquid of BS1 showed the following. The sedimentation rate of activated sludge increased significantly at the beginning of the process upon adding a BS1 cell suspension, while the introduction of the native solution of BS1 intensified the process following 5 minutes of contact. The obtained experimental data suggest that the media containing extracellular bacterial lectins can be effectively used as a coagulant (flocculant) for the sedimentation of activated sludge.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90711554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-06DOI: 10.21285/2227-2925-2021-11-4-523-530
D. B. Thin, V. Thanh, B. B. Thinh, Ба Тхин, Ву Кует Тхань, Буй Бао Тхинь
Abstract. Recent years have seen the development of bacterial resistance to currently available antibiotics, which necessitates a search for new antimicrobial agents. Amomum muricarpum Elmer is a widely used medicinal plant species in the genus Amomum (family Zingiberaceae) that is commonly found in Laos, the Philippines, China, and Vietnam. The present article describes the chemical composition and antimicrobial activity of essential oils extracted from the leaves and rhizomes of A. muricarpum from North Vietnam. The hydrodistilled essential oil was analyzed using gas chromatography and gas chromatography-mass spectrometry, with the broth microdilution method designed to evaluate its antimicrobial efficacy. The absolute yield of essential oils amounted to 0.11% and 0.13% (v/w) for leaves and rhizomes, respectively, on a dry weight basis. It was found that the leaves and rhizomes of A. muricarpum produce oils abounding in monoterpenes. Of the total identified volatile components in the leaf oil (97.18%), three main constituents include α-pinene (40.45%), linalool (12.34%), and β-pinene (10.31%). In the rhizome oil, the main constituents include α-pinene (48.10%), β-pinene (20.32%), and linalool (7.56%) of the total identified volatile components (98.08%). An antimicrobial activity test indicates that essential oils from the leaves and rhizome of A. muricarpum inhibit the growth of Staphylococcus aureus ATCC 25923, with a minimum inhibitory concentration (MIC) of 200 µg/ml. In addition, the rhizome essential oil also exhibits antimicrobial activity against Bacillus cereus ATCC 14579, with a MIC value of 200 µg/ml. The results indicate the potential of essential oils extracted from A. muricarpum as a source of antimicrobial agents.
{"title":"Chemical composition and antimicrobial activity of essential oils extracted from Amomum muricarpum Elmer from North Vietnam","authors":"D. B. Thin, V. Thanh, B. B. Thinh, Ба Тхин, Ву Кует Тхань, Буй Бао Тхинь","doi":"10.21285/2227-2925-2021-11-4-523-530","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-523-530","url":null,"abstract":"Abstract. Recent years have seen the development of bacterial resistance to currently available antibiotics, which necessitates a search for new antimicrobial agents. Amomum muricarpum Elmer is a widely used medicinal plant species in the genus Amomum (family Zingiberaceae) that is commonly found in Laos, the Philippines, China, and Vietnam. The present article describes the chemical composition and antimicrobial activity of essential oils extracted from the leaves and rhizomes of A. muricarpum from North Vietnam. The hydrodistilled essential oil was analyzed using gas chromatography and gas chromatography-mass spectrometry, with the broth microdilution method designed to evaluate its antimicrobial efficacy. The absolute yield of essential oils amounted to 0.11% and 0.13% (v/w) for leaves and rhizomes, respectively, on a dry weight basis. It was found that the leaves and rhizomes of A. muricarpum produce oils abounding in monoterpenes. Of the total identified volatile components in the leaf oil (97.18%), three main constituents include α-pinene (40.45%), linalool (12.34%), and β-pinene (10.31%). In the rhizome oil, the main constituents include α-pinene (48.10%), β-pinene (20.32%), and linalool (7.56%) of the total identified volatile components (98.08%). An antimicrobial activity test indicates that essential oils from the leaves and rhizome of A. muricarpum inhibit the growth of Staphylococcus aureus ATCC 25923, with a minimum inhibitory concentration (MIC) of 200 µg/ml. In addition, the rhizome essential oil also exhibits antimicrobial activity against Bacillus cereus ATCC 14579, with a MIC value of 200 µg/ml. The results indicate the potential of essential oils extracted from A. muricarpum as a source of antimicrobial agents.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81692845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-06DOI: 10.21285/2227-2925-2021-11-4-531-539
Yulia A. Golubeva, K. S. Smirnova, L. Klyushova, V. Potkin, E. Lider
Oligopyridine based copper(II) complexes are of interest to scientists as possible anticancer agents due to promising cytotoxic and DNA binding/cleaving properties. In this study, copper(II) complex [Cu(phendione)L2]·C2H5OH with 1,10-phenanthroline-5,6-dione (phendione) and 4,5-dichloro-isothiazole-3-carboxylic acid (HL) was synthesized and characterized by elemental analysis, IR-spectroscopy, X-ray powder diffraction and single-crystal X-ray diffraction. According to X-ray diffraction data, obtained compound is mononuclear complex with square pyramidal coordination environment of the central atom which is surrounded by two isothiazolate molecules and one phendione ligand. The X-ray diffraction data are confirmed by IR-spectroscopy data showing the presence of characteristic stretching vibration bands of the carbonyl and carboxyl groups of oligopyridine ligand and isothiazolate ions, respectively. Density functional theory (DFT) calculations for complex were carried out using the ADF software package to perform geometry optimization and frequency calculations that were in a good agreement with experimental IR spectrum. Cytotoxicity of complex and initial reagents was tested in vitro against HepG2 (human hepatocellular carcinoma) and MCF-7 (human breast adenocarcinoma) cell lines. The complex showed high dose-dependent cytotoxic activity with the IC50 values of 0.60±0.03 µM and 0.96±0.13 µM, respectively, which is higher than the activity of cisplatin against these cell lines. The activity of the complex is due to the presence of phendione ligand, which exhibits a similar cytotoxic activity.
{"title":"Synthesis and crystal structure of cytotoxic copper(II) complex with 1,10-phenanthroline-5,6-dione and isothiazole derivative","authors":"Yulia A. Golubeva, K. S. Smirnova, L. Klyushova, V. Potkin, E. Lider","doi":"10.21285/2227-2925-2021-11-4-531-539","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-531-539","url":null,"abstract":"Oligopyridine based copper(II) complexes are of interest to scientists as possible anticancer agents due to promising cytotoxic and DNA binding/cleaving properties. In this study, copper(II) complex [Cu(phendione)L2]·C2H5OH with 1,10-phenanthroline-5,6-dione (phendione) and 4,5-dichloro-isothiazole-3-carboxylic acid (HL) was synthesized and characterized by elemental analysis, IR-spectroscopy, X-ray powder diffraction and single-crystal X-ray diffraction. According to X-ray diffraction data, obtained compound is mononuclear complex with square pyramidal coordination environment of the central atom which is surrounded by two isothiazolate molecules and one phendione ligand. The X-ray diffraction data are confirmed by IR-spectroscopy data showing the presence of characteristic stretching vibration bands of the carbonyl and carboxyl groups of oligopyridine ligand and isothiazolate ions, respectively. Density functional theory (DFT) calculations for complex were carried out using the ADF software package to perform geometry optimization and frequency calculations that were in a good agreement with experimental IR spectrum. Cytotoxicity of complex and initial reagents was tested in vitro against HepG2 (human hepatocellular carcinoma) and MCF-7 (human breast adenocarcinoma) cell lines. The complex showed high dose-dependent cytotoxic activity with the IC50 values of 0.60±0.03 µM and 0.96±0.13 µM, respectively, which is higher than the activity of cisplatin against these cell lines. The activity of the complex is due to the presence of phendione ligand, which exhibits a similar cytotoxic activity.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82541571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-06DOI: 10.21285/2227-2925-2021-11-4-540-548
A. Stolbikov, R. Salyaev, N. Rekoslavskaya
The present study aims to develop and test procedures for detecting the DNA of dangerous human papillomaviruses (HPV) types 6 and 16 in water samples. The conserved segments of HPV 6 L1 and HPV 16 L1 nucleic acid sequences were studied using bioinformatic methods with the help of the NCBI (National Center for Biotechnology Information) database and the BioEdit program. A total of 135 nucleic acid sequences of HPV6 L1 and 945 nucleic acid sequences of HPV16 L1 were examined. Five pairs of specific primers were developed for the identified conserved segments of nucleic acid sequences using specialized programs (PerlPrimer v.1.1.21, FastPCR 6.6, and Primer3Plus). In addition, several procedures for collecting samples from various water bodies located near Listvyanka settlement (Lake Baikal) were tested. The samples were subjected to comprehensive purification from insoluble particles and bacterial contamination to be tested for the presence of HPV DNA via PCR analysis using primers complementary to the nucleic acid sequences of HPV6 L1 and HPV16 L1. The conducted studies revealed HPV 6 and HPV 16 DNA in the water samples. Due to the use of the developed and tested procedures for collecting and examining samples from various water sources in the Baikal Natural Territory followed by a PCR analysis, it was possible to detect the presence of dangerous viruses. The proposed procedure of testing water samples for the presence of HPV can be useful in developing effective monitoring of water bodies and wastewater both in Baikal and other regions.
本研究旨在开发和测试检测水样中危险的人类乳头瘤病毒(HPV) 6型和16型DNA的程序。在NCBI (National Center for Biotechnology Information)数据库和BioEdit程序的帮助下，采用生物信息学方法研究了HPV 6l1和HPV 16l1核酸序列的保守片段。共检测了135个HPV6 L1核酸序列和945个HPV16 L1核酸序列。利用专用程序(PerlPrimer v.1.1.21、FastPCR 6.6和Primer3Plus)对鉴定出的核酸序列保守片段开发5对特异性引物。此外，还测试了从Listvyanka定居点(贝加尔湖)附近的各种水体中收集样本的几种程序。对样品进行不溶性颗粒和细菌污染的全面纯化，利用与HPV6 L1和HPV16 L1核酸序列互补的引物进行PCR分析，检测HPV DNA的存在。所进行的研究显示，水样中含有HPV 6和HPV 16 DNA。由于使用了开发和测试的程序，从贝加尔湖自然领土的各种水源收集和检查样本，然后进行聚合酶链反应分析，因此有可能检测到危险病毒的存在。拟议的检测水样是否存在HPV的程序可用于对贝加尔湖和其他地区的水体和废水进行有效监测。
{"title":"Development and testing of procedures for detecting HPV 6 and HPV 16 DNA in water samples","authors":"A. Stolbikov, R. Salyaev, N. Rekoslavskaya","doi":"10.21285/2227-2925-2021-11-4-540-548","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-540-548","url":null,"abstract":"The present study aims to develop and test procedures for detecting the DNA of dangerous human papillomaviruses (HPV) types 6 and 16 in water samples. The conserved segments of HPV 6 L1 and HPV 16 L1 nucleic acid sequences were studied using bioinformatic methods with the help of the NCBI (National Center for Biotechnology Information) database and the BioEdit program. A total of 135 nucleic acid sequences of HPV6 L1 and 945 nucleic acid sequences of HPV16 L1 were examined. Five pairs of specific primers were developed for the identified conserved segments of nucleic acid sequences using specialized programs (PerlPrimer v.1.1.21, FastPCR 6.6, and Primer3Plus). In addition, several procedures for collecting samples from various water bodies located near Listvyanka settlement (Lake Baikal) were tested. The samples were subjected to comprehensive purification from insoluble particles and bacterial contamination to be tested for the presence of HPV DNA via PCR analysis using primers complementary to the nucleic acid sequences of HPV6 L1 and HPV16 L1. The conducted studies revealed HPV 6 and HPV 16 DNA in the water samples. Due to the use of the developed and tested procedures for collecting and examining samples from various water sources in the Baikal Natural Territory followed by a PCR analysis, it was possible to detect the presence of dangerous viruses. The proposed procedure of testing water samples for the presence of HPV can be useful in developing effective monitoring of water bodies and wastewater both in Baikal and other regions.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"267 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73327550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-06DOI: 10.21285/2227-2925-2021-11-4-508-516
J. Bazarova, A. V. Logvinova, B. Bazarov
A fundamental problem in materials science consists in establishing a relationship between the chemical composition, structure, and properties of materials. This issue can be solved through the study of multicomponent systems and the directed synthesis of promising compounds. Of practical interest here are active dielectrics that are based on complex oxide compounds, specifically molybdates. Among complex molybdates and tungstates, ternary caged molybdates of the following structural types are of greatest importance: nasicon, perovskite, langbeinite, etc. Due to their widely varying elemental and quantitative compositions, such molybdates are convenient models for structural and chemical design, as well as the establishment of “composition–structure– properties” genetic relationships. Bismuth-containing complex molybdate systems exhibit the formation of phases having ferro-piezoelectric, ionic, and other properties. In this work, the Rb2MoO4–Bi2(MoO4)3–Zr(MoO4)2 ter nary salt system was studied for the first time using the method of intersecting sections in the subsolidus region (450–650 ℃). To this end, quasibinary sections were identified; triangulation was performed. Ternary molybdates Rb5BiZr(MoO4)6 and Rb2BiZr2(MoO4)6,5 were formed in the system using a ceramic technology. These compounds are isostructural to the previously obtained REE molybdates (M5LnZr(MoO4)6) but contain trivalent bismuth instead of rare earth elements. The structure of Rb5BiZr(MoO4)6 was adjusted via the Rietveld refinement technique using the TOPAS 4.2 software package. The ternary molybdate crystallizes in a trigonal system, with the following unit cell parameters of the R`3c space group: a = 10.7756(2) and c = 39.0464(7) Å. According to the studies of thermal properties exhibited by M5BiZr(MoO4)6, these ternary molybdates undergo the first-order phase transition in the temperature range of 450–600 ºC. The IR and Raman spectra of M5BiZr(MoO4)6 reveal the crystallization of ternary molybdates in the R`3c space group. The conducted comparative characterization of M2MoO4–Bi2(MoO4)3–Zr(MoO4)2 phase diagrams suggests that the phase equilibria of these systems depend on the nature of molybdates of monovalent elements.
{"title":"Interactions in ternary bismuth-containing molybdate systems M2MoO4-Bi2(MoO4)3-Zr(MoO4)2 in the subsolidus region","authors":"J. Bazarova, A. V. Logvinova, B. Bazarov","doi":"10.21285/2227-2925-2021-11-4-508-516","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-4-508-516","url":null,"abstract":"A fundamental problem in materials science consists in establishing a relationship between the chemical composition, structure, and properties of materials. This issue can be solved through the study of multicomponent systems and the directed synthesis of promising compounds. Of practical interest here are active dielectrics that are based on complex oxide compounds, specifically molybdates. Among complex molybdates and tungstates, ternary caged molybdates of the following structural types are of greatest importance: nasicon, perovskite, langbeinite, etc. Due to their widely varying elemental and quantitative compositions, such molybdates are convenient models for structural and chemical design, as well as the establishment of “composition–structure– properties” genetic relationships. Bismuth-containing complex molybdate systems exhibit the formation of phases having ferro-piezoelectric, ionic, and other properties. In this work, the Rb2MoO4–Bi2(MoO4)3–Zr(MoO4)2 ter nary salt system was studied for the first time using the method of intersecting sections in the subsolidus region (450–650 ℃). To this end, quasibinary sections were identified; triangulation was performed. Ternary molybdates Rb5BiZr(MoO4)6 and Rb2BiZr2(MoO4)6,5 were formed in the system using a ceramic technology. These compounds are isostructural to the previously obtained REE molybdates (M5LnZr(MoO4)6) but contain trivalent bismuth instead of rare earth elements. The structure of Rb5BiZr(MoO4)6 was adjusted via the Rietveld refinement technique using the TOPAS 4.2 software package. The ternary molybdate crystallizes in a trigonal system, with the following unit cell parameters of the R`3c space group: a = 10.7756(2) and c = 39.0464(7) Å. According to the studies of thermal properties exhibited by M5BiZr(MoO4)6, these ternary molybdates undergo the first-order phase transition in the temperature range of 450–600 ºC. The IR and Raman spectra of M5BiZr(MoO4)6 reveal the crystallization of ternary molybdates in the R`3c space group. The conducted comparative characterization of M2MoO4–Bi2(MoO4)3–Zr(MoO4)2 phase diagrams suggests that the phase equilibria of these systems depend on the nature of molybdates of monovalent elements.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82991571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-07DOI: 10.21285/2227-2925-2021-11-3-421-429
Yu. D. Smirnova, G. Rabinovich, N. Fomicheva
Abstract: The recent decades have witnessed a significant development and implementation of nanotechnology, including in various branches of agriculture. There is an active search for ways to obtain preparations for plant growing with nanoparticles that can be more rapidly involved in the metabolic processes of plants. This article aims to obtain a nanosized silica-humic preparation and its approbation on potato plants. As a source of humic substances, a liquid humic preparation BoGum (developed by the All-Russian Research Institute of Reclaimed Lands) was used, as a source of silicon – sodium metasilicate. Ultrasonic dispersion method was used for achieving the nanoscale of the samples. A silicon source was introduced in an amount of 0.1% (of SiO2) into BoGum, followed by the application of ultrasonic action for 5, 10, 15 and 20 minutes. The analysis of the obtained samples using a 90 Plus/MAS particle size analyzer has shown that with increasing dispersion time, the effective particle diameter changed insignificantly. At the same time, a redistribution of particles was noted: when the samples were exposed for 20 minutes, the number of smaller particles increased. After 5 minutes of treatment, the range of particle distribution was 115±13–830±23 nm, after 20 minutes of exposure, the particle diameter fell into two regions: 81±8–120±10 and 280±4–470±18 nm. Ultrasonic action contributed to the retention of the stable state of aggregation of the obtained preparation, larger microbiological activity and larger content of humic acid in comparison with the silica-humic preparation, obtained without the application of an ultrasound. The new nanosized silica-humic preparation has been tested on potato plants. Treatment of tubers before planting, followed by foliar spraying of vegetative plants, has contributed to an increase in potato yield by 18.7%. Changes were noted in the content of mono- and polysilicic acids in the soil, as well as the accumulation of silicon in the tops of potatoes when using silicahumic preparations by 0.96% of absolute dry mass on average.
{"title":"Obtaining a nanosized silica-humic preparation and its initial approbation","authors":"Yu. D. Smirnova, G. Rabinovich, N. Fomicheva","doi":"10.21285/2227-2925-2021-11-3-421-429","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-3-421-429","url":null,"abstract":"Abstract: The recent decades have witnessed a significant development and implementation of nanotechnology, including in various branches of agriculture. There is an active search for ways to obtain preparations for plant growing with nanoparticles that can be more rapidly involved in the metabolic processes of plants. This article aims to obtain a nanosized silica-humic preparation and its approbation on potato plants. As a source of humic substances, a liquid humic preparation BoGum (developed by the All-Russian Research Institute of Reclaimed Lands) was used, as a source of silicon – sodium metasilicate. Ultrasonic dispersion method was used for achieving the nanoscale of the samples. A silicon source was introduced in an amount of 0.1% (of SiO2) into BoGum, followed by the application of ultrasonic action for 5, 10, 15 and 20 minutes. The analysis of the obtained samples using a 90 Plus/MAS particle size analyzer has shown that with increasing dispersion time, the effective particle diameter changed insignificantly. At the same time, a redistribution of particles was noted: when the samples were exposed for 20 minutes, the number of smaller particles increased. After 5 minutes of treatment, the range of particle distribution was 115±13–830±23 nm, after 20 minutes of exposure, the particle diameter fell into two regions: 81±8–120±10 and 280±4–470±18 nm. Ultrasonic action contributed to the retention of the stable state of aggregation of the obtained preparation, larger microbiological activity and larger content of humic acid in comparison with the silica-humic preparation, obtained without the application of an ultrasound. The new nanosized silica-humic preparation has been tested on potato plants. Treatment of tubers before planting, followed by foliar spraying of vegetative plants, has contributed to an increase in potato yield by 18.7%. Changes were noted in the content of mono- and polysilicic acids in the soil, as well as the accumulation of silicon in the tops of potatoes when using silicahumic preparations by 0.96% of absolute dry mass on average.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73620769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-07DOI: 10.21285/2227-2925-2021-11-3-384-392
E. Serba, L. Rimareva, M. Overchenko, N. Ignatova, M. Medrish, A. A. Pavlova, E. Sokolova
Abstract: Development of sustainable biotechnologies for deep processing of grain raw materials requires effective mechanisms of obtaining strong wort for alcohol production. To provide qualitative characteristics of biochemical composition and rheological properties of strong wort, it is necessary to select optimal enzyme systems and conditions for deep conversion of high-molecular weight polymers of the grain. Previous research has proven the efficiency of carbohydrases for processing grain raw materials. However, there is little evidence on the catalytic effect of phytase, including in combination with other hydrolytic enzymes, on the degree of hydrolysis of polymers in grain raw materials when preparing strong wort. This paper demonstrates the effect of proteases and phytases in a multi-enzyme composition, as well as the conditions of enzymatic processing of raw materials, on the rheological and biochemical parameters of strong wort. Wheat, rye and corn were investigated. The synergism of the combined effect of studied hydrolases, including phytolytic and proteolytic enzymes, contributed to an increase in polymer conversion in this grain raw material and the concentration of soluble dry substances of the wort by 1.5 times. Using the proteases and phytases in the multienzyme composition allowed the concentration of the following components in the wort to be increased: glucose – by 1.2–1.3 times; amine nitrogen – by 1.5–2.2 times; phosphorus ions – by 1.4–4.3 times. Additionally, in the wort samples, the content of amino acids in the free form increased by over 4 times. It is shown that the pretreatment of grain raw materials at a temperature of 80–90ºС for 6 hours and saccharification for 1–2 hours using a complete complex of enzymes containing α-amylase, glucoamylase, xylanase, protease and phytase, allows a strong wort with a dry matter content of over 30% to be obtained. Moreover, a significant decrease in viscosity was noted (particularly for rye wort – by 1.3–1.9 times). Our results confirm the essential role of enzymes exhibiting substrate specificity to protein and phytic polymers in grain raw materials.
{"title":"Selecting multi-enzyme composition and preparation conditions for strong wort","authors":"E. Serba, L. Rimareva, M. Overchenko, N. Ignatova, M. Medrish, A. A. Pavlova, E. Sokolova","doi":"10.21285/2227-2925-2021-11-3-384-392","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-3-384-392","url":null,"abstract":"Abstract: Development of sustainable biotechnologies for deep processing of grain raw materials requires effective mechanisms of obtaining strong wort for alcohol production. To provide qualitative characteristics of biochemical composition and rheological properties of strong wort, it is necessary to select optimal enzyme systems and conditions for deep conversion of high-molecular weight polymers of the grain. Previous research has proven the efficiency of carbohydrases for processing grain raw materials. However, there is little evidence on the catalytic effect of phytase, including in combination with other hydrolytic enzymes, on the degree of hydrolysis of polymers in grain raw materials when preparing strong wort. This paper demonstrates the effect of proteases and phytases in a multi-enzyme composition, as well as the conditions of enzymatic processing of raw materials, on the rheological and biochemical parameters of strong wort. Wheat, rye and corn were investigated. The synergism of the combined effect of studied hydrolases, including phytolytic and proteolytic enzymes, contributed to an increase in polymer conversion in this grain raw material and the concentration of soluble dry substances of the wort by 1.5 times. Using the proteases and phytases in the multienzyme composition allowed the concentration of the following components in the wort to be increased: glucose – by 1.2–1.3 times; amine nitrogen – by 1.5–2.2 times; phosphorus ions – by 1.4–4.3 times. Additionally, in the wort samples, the content of amino acids in the free form increased by over 4 times. It is shown that the pretreatment of grain raw materials at a temperature of 80–90ºС for 6 hours and saccharification for 1–2 hours using a complete complex of enzymes containing α-amylase, glucoamylase, xylanase, protease and phytase, allows a strong wort with a dry matter content of over 30% to be obtained. Moreover, a significant decrease in viscosity was noted (particularly for rye wort – by 1.3–1.9 times). Our results confirm the essential role of enzymes exhibiting substrate specificity to protein and phytic polymers in grain raw materials.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83714806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-07DOI: 10.21285/2227-2925-2021-11-3-441-448
A. S. Burlachenko, O. Salishcheva, L. Dyshlyuk
Abstract: The paper examines the biodegradation rate of cocamidopropyl betaine by bacteria of the genus Pseudomonas and activated sludge. The following microorganisms were taken as destructor strains: Pseudomonas fluorescens TR (VKPM B-4881), Pseudomonas putida TP-19 (B-6582), Pseudomonas stutzeri T (B-4904), Pseudomonas putida TSh-18 (B-2950), Pseudomonas putida TO (B-3959), Pseudomonas mendocina 2S (B-4710), Pseudomonas oleovorans TF4-1L (B-8621) and activated sludge obtained at activated sludge reactors of a Kuzbass plant. Biooxidation of surfactant samples was carried out in 250 cm3 glass flasks, placed into an incubator shaker, at a constant temperature of 30ºС for pure cultures and 18ºС for activated sludge. The destructor strain should reduce the surfactant concentration to safe values within a minimum time interval. Pseudomonas stutzeri T (B-4904) and Pseudomonas fluorescens TR (B-4881) strains provided the shortest half-life of the surfactant under study – 2.5 and 2.6 days, respectively. For Pseudomonas putida TO (B-3959), Pseudomonas putida TSh-18 (B-2950) and Pseudomonas oleovorans TF4-1L (B-8621) strains, these values amounted to 3.0, 4.5 and 4.9 days, respectively. The maximum half-life of the surfactant under study was demonstrated by Pseudomonas mendocina 2S (B-4710) and Pseudomonas putida TP-19 (B-6582) microorganisms – 5.5 and 6.0 days, respectively. The maximum biodegradation of the surfactant was observed under its exposure to the biocenosis of microorganisms. Over 14 days, the concentration of cocamidopropyl betaine decreased to 0.27% of its initial concentration. The efficiency of Pseudomonas bacteria as destructors of surfactants was demonstrated. Bacteria of this genus exhibit a shorter generation time and a higher rate of biomass growth when compared to other strains and a shorter period of adaptation to surfactants when compared to activated sludge. Capable of reducing surfactant concentrations to safe values in a minimum time interval, Pseudomonas strains can be used as an effective agent in the development of technologies for wastewater purification from amphoteric surfactants.
{"title":"Study into biodegradation of cocamidopropyl betaine, an amphoteric surfactant, by Pseudomonas bacteria and activated sludge","authors":"A. S. Burlachenko, O. Salishcheva, L. Dyshlyuk","doi":"10.21285/2227-2925-2021-11-3-441-448","DOIUrl":"https://doi.org/10.21285/2227-2925-2021-11-3-441-448","url":null,"abstract":"Abstract: The paper examines the biodegradation rate of cocamidopropyl betaine by bacteria of the genus Pseudomonas and activated sludge. The following microorganisms were taken as destructor strains: Pseudomonas fluorescens TR (VKPM B-4881), Pseudomonas putida TP-19 (B-6582), Pseudomonas stutzeri T (B-4904), Pseudomonas putida TSh-18 (B-2950), Pseudomonas putida TO (B-3959), Pseudomonas mendocina 2S (B-4710), Pseudomonas oleovorans TF4-1L (B-8621) and activated sludge obtained at activated sludge reactors of a Kuzbass plant. Biooxidation of surfactant samples was carried out in 250 cm3 glass flasks, placed into an incubator shaker, at a constant temperature of 30ºС for pure cultures and 18ºС for activated sludge. The destructor strain should reduce the surfactant concentration to safe values within a minimum time interval. Pseudomonas stutzeri T (B-4904) and Pseudomonas fluorescens TR (B-4881) strains provided the shortest half-life of the surfactant under study – 2.5 and 2.6 days, respectively. For Pseudomonas putida TO (B-3959), Pseudomonas putida TSh-18 (B-2950) and Pseudomonas oleovorans TF4-1L (B-8621) strains, these values amounted to 3.0, 4.5 and 4.9 days, respectively. The maximum half-life of the surfactant under study was demonstrated by Pseudomonas mendocina 2S (B-4710) and Pseudomonas putida TP-19 (B-6582) microorganisms – 5.5 and 6.0 days, respectively. The maximum biodegradation of the surfactant was observed under its exposure to the biocenosis of microorganisms. Over 14 days, the concentration of cocamidopropyl betaine decreased to 0.27% of its initial concentration. The efficiency of Pseudomonas bacteria as destructors of surfactants was demonstrated. Bacteria of this genus exhibit a shorter generation time and a higher rate of biomass growth when compared to other strains and a shorter period of adaptation to surfactants when compared to activated sludge. Capable of reducing surfactant concentrations to safe values in a minimum time interval, Pseudomonas strains can be used as an effective agent in the development of technologies for wastewater purification from amphoteric surfactants.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90065597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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