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Dominating taxonomic composition of the early life gut microbiota and influencing factors in infants up to seven months of age in Latvia 主导的早期生命肠道微生物群的分类学组成和影响因素在拉脱维亚婴儿7个月的年龄
Q4 Multidisciplinary Pub Date : 2022-12-01 DOI: 10.2478/prolas-2022-0101
Egija Zelča, D. Gudrā, Ērika Lūse, Jana Peterleviča, M. Ustinova, D. Fridmanis, I. Rumba-Rozenfelde, I. Daugule
Abstract It has been hypothesised that the establishment of stable adult microbiota is programmed in infancy, and therefore early life gut colonisation may lead to a lifelong microbiota pattern with significant effects on health. The aim of the study was to analyse the composition of gut microbiota and influencing factors in infants up to seven months of age in Latvia. A cross-sectional study was performed at primary healthcare centres. The parents of healthy infants filled out a questionnaire and brought the child’s faecal sample. 16 rRNS gene sequencing was performed to identify the bacterial taxonomic units. The composition of gut microbiota was compared between children with different risk factors. The final participant sample group included 55 infants with median age 4.0 months. The infant gut microbiota of the sample group had typical and rather healthy microbiota — the main phyla detected were Firmicutes and Actinobacteria, the main family was Bifidobacteriacea and genus — Bifidobacterium. A significant effect of the type of delivery and feeding type was identified, as well as negative correlation between Lactobacilli and gestational age. Further, it would be important to analyse the changes of microbiota prospectively to identify the association with environmental factors and health status in dynamics.
据推测,稳定的成人微生物群的建立是在婴儿期进行的,因此早期肠道定植可能导致终生的微生物群模式,对健康产生重大影响。该研究的目的是分析拉脱维亚7个月以下婴儿肠道微生物群的组成及其影响因素。在初级保健中心进行了横断面研究。健康婴儿的父母填写了一份调查问卷,并带来了孩子的粪便样本。16个rRNS基因测序鉴定细菌的分类单位。比较不同危险因素患儿肠道菌群组成。最终参与者样本组包括55名中位年龄为4.0个月的婴儿。样本组婴儿肠道菌群具有典型且较为健康的菌群,检出的主要门为厚壁菌门和放线菌门,主要科为双歧杆菌科和双歧杆菌属。分娩方式和喂养方式对乳酸菌数量有显著影响,乳酸菌数量与胎龄呈负相关。此外,对微生物群的变化进行前瞻性分析,以确定其与环境因素和健康状况的动态关系具有重要意义。
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引用次数: 0
Surgical and non-surgical treatment of paediatric appendicitis: can algorithms help us to predict perforation? 小儿阑尾炎的手术和非手术治疗:算法能帮助我们预测穿孔吗?
Q4 Multidisciplinary Pub Date : 2022-12-01 DOI: 10.2478/prolas-2022-0092
A. Eņģelis, M. Kakar, A. Zviedre, P. Laizāns, Timurs Zurmutai, J. Bormotovs, A. Petersons
Abstract The recent interest in and evidence of non-surgical treatment with antibiotic therapy has led to the recurring issue of differentiating acute no-complicated appendicitis (AnA) and acute complicated appendicitis (AcA) when these are presented in an emergency department. To create the initial version of an acute appendicitis (AA) diagnostic and treatment algorithm, we analysed treatment results of 178 children with AnA and AcA treated at the Children’s Clinical University Hospital in Rīga, in the period between 2010 and 2013. Evaluation of the clinical symptoms, laboratory and radiological findings was included in development of the algorithm. The algorithm was created in 2016 and accepted by the hospital administration. We present the algorithm’s updated version of 2020. The introduction of diagnostic scores and algorithms has standardised and improved the diagnosis of paediatric AA. New diagnostic tests with higher sensitivity and specificity may improve the accuracy of diagnostic algorithms. Measuring multiple effective biomarkers simultaneously may improve the accuracy of diagnostic algorithms and predict the severity of paediatric AA. Machine learning algorithms may be able to process a much larger amount of data and provide a faster conclusion, helping the surgeon make the right decision in diagnosing appendicitis in children and prevent unnecessary surgery.
摘要近年来对非手术治疗抗生素治疗的兴趣和证据导致了急性无并发症阑尾炎(AnA)和急性并发症阑尾炎(AcA)在急诊科出现时的反复区分问题。为了创建急性阑尾炎(AA)诊断和治疗算法的初始版本,我们分析了2010年至2013年期间在r ? ga儿童临床大学医院治疗的178名AnA和AcA儿童的治疗结果。该算法的开发包括对临床症状、实验室和放射检查结果的评估。该算法于2016年创建,并被医院管理层接受。我们给出了2020年算法的更新版本。诊断评分和算法的引入规范和提高了儿科AA的诊断。新的诊断测试具有更高的灵敏度和特异性,可以提高诊断算法的准确性。同时测量多种有效的生物标志物可以提高诊断算法的准确性,并预测儿科AA的严重程度。机器学习算法可能能够处理大量数据并提供更快的结论,帮助外科医生在诊断儿童阑尾炎时做出正确的决定,并防止不必要的手术。
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引用次数: 0
Impact of body mass index on parameters of the left atrium: cardiac computed tomography study 身体质量指数对左心房参数的影响:心脏计算机断层扫描研究
Q4 Multidisciplinary Pub Date : 2022-12-01 DOI: 10.2478/prolas-2022-0098
Viktorija Verhovceva, L. Zvaigzne, K. Jubele, O. Kalējs
Abstract Increased Body Mass Index (BMI) is often used as a predictor for cardiovascular diseases, and it is also known to be associated with left atrial enlargement, potentially affecting anatomic structures of the left atrium. The aim of the study was to determine the association between BMI and parameters of the left atrium, including characteristics of the pulmonary veins (PVs), found in cardiac computed tomography (CT) scan. The retrospective study included 140 patients with a mean age of 66.9 years (SD = 7.8). There were statistically significant correlations between BMI and volume changes of the left atrium during the cardiac cycle: Vmax (rs = 0.199, p = 0.023), Vmin (rs = 0.177, p = 0.043), Vmean (rs = 0.190, p = 0.029), which supports previously known data. The study also revealed unique associations regarding the impact of BMI on PV structures. There was a significant correlation between BMI and PV orifice size in the left inferior PV (rs = 0.216, p = 0.032) and the right accessory PV (rs = –0.629, p = 0.012). The right PV angle was positively correlated with BMI (rs = 0.178, p = 0.044), while the angle between left PVs did not show any correlation with BMI (p = 0.436). The results support previously known associations between increased BMI and left atrial enlargement and show a statistically significant effect of increased BMI on the characteristics of pulmonary veins.
身体质量指数(BMI)升高常被用作心血管疾病的预测指标,也被认为与左心房扩大有关,可能影响左心房的解剖结构。该研究的目的是确定BMI与左心房参数之间的关系,包括在心脏计算机断层扫描(CT)中发现的肺静脉(pv)特征。回顾性研究纳入140例患者,平均年龄66.9岁(SD = 7.8)。心周期内BMI与左心房容积变化的相关性有统计学意义:Vmax (rs = 0.199, p = 0.023)、Vmin (rs = 0.177, p = 0.043)、Vmean (rs = 0.190, p = 0.029),支持已有数据。该研究还揭示了BMI对PV结构影响的独特关联。BMI与左侧下PV和右侧副PV孔大小有显著相关性(rs = 0.216, p = 0.032)。右侧PV角度与BMI呈正相关(rs = 0.178, p = 0.044),左侧PV角度与BMI无相关性(p = 0.436)。该结果支持了先前已知的BMI增加与左心房增大之间的关联,并显示BMI增加对肺静脉特征的统计显着影响。
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引用次数: 1
Life Cycle Assessment for Olive Production: A Case Study for the Region of Trás-os-Montes, Portugal 橄榄生产的生命周期评估:葡萄牙Trás-os-Montes地区的案例研究
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0081
T. Brito, A. Fernandes-Silva
Abstract The olive tree (Olea europaea L.), widely cultivated in the Mediterranean countries for olive oil and table olive production, has an important economic role. In the age of climate change, it is crucial to know the importance of environmental impacts associated to the production system. Thus, the aim of this study was to characterise the environmental impacts of a semi-intensive olive orchard drip irrigated with ground water, located in the Northeast Region of Portugal. Life cycle analysis was conducted according to ISO 14040. We used openLCA software, the Agribalyse V. 3 database, and a cradle-to-gate analysis in the impact assessment (Recipe Midpoint (H) V1.13). In the climate change category, the results indicated that for production of 1 kg olives, a total of 0.249 kg CO2-Eq. were emitted to the atmosphere. Regarding the direct impact of production system, a total of 0.177 kg CO2-Eq. (71.5%) were emitted, followed by indirect impacts associated with raw material of the irrigation system (0.032 kg CO2-Eq. total, 12.7%), and electricity needed to pump ground water (0.012 kg CO2-Eq., 4.8%). In relation to inputs for production, the raw material for urea production contributed 0.013 kg CO2-Eq. (5.0%) of total emissions. To the best of our knowledge, this study was the first carried out in this region, and contributes to a better understanding of environmental impacts that will allow their minimisation and ensure olive orchard sustainability.
橄榄树(Olea europaea L.)在地中海国家广泛种植,用于生产橄榄油和食用橄榄,具有重要的经济作用。在气候变化时代,了解与生产系统相关的环境影响的重要性至关重要。因此,本研究的目的是描述位于葡萄牙东北部地区的地下滴灌半集约化橄榄园对环境的影响。生命周期分析按照ISO 14040进行。我们在影响评估中使用了openLCA软件、Agribalyse V. 3数据库和从摇篮到大门的分析(Recipe Midpoint (H) V1.13)。在气候变化范畴,结果表明,生产1公斤橄榄,总共0.249公斤二氧化碳当量。被排放到大气中。对于生产系统的直接影响,共0.177 kg CO2-Eq。(71.5%),其次是与灌溉系统原料相关的间接影响(0.032 kg co2当量)。总共12.7%),以及抽取地下水所需的电力(0.012千克二氧化碳当量)。, 4.8%)。在生产投入方面,尿素生产的原料贡献了0.013千克二氧化碳当量。占总排放量的5.0%。据我们所知,这项研究是第一次在该地区进行,有助于更好地了解环境影响,从而最大限度地减少环境影响,并确保橄榄园的可持续性。
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引用次数: 0
Mechanical Thinning Improves the Consistency of Flowering and Yield in Apple Production 机械间伐提高苹果开花和产量的一致性
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0084
E. Rubauskis, Dāniels Udalovs, I. Borisova
Abstract Most apple cultivars tend to bear biennially, i.e. produce many fruits in one and few fruits in the next year, or do not have a nature of self-thinning. The effectiveness of chemical thinning depends on conditions, particularly temperature, at the time of application. In some other fruit-growing regions, the mechanical thinning of flowers is adapted to some cultivars by changing the speed of moving and rotation of the mechanisms to conduct partial thinning — removing of flowers. The investigation aimed to evaluate the effectiveness of total flower removal mechanically on one side of the tree row, and on the other side the next year, during the full production period (8th to 12th year after planting) to obtain regular yields. Seven cultivars on dwarfing rootstocks M.9 and B.396 were compared. Apple trees on the dwarfing rootstock B.396 were less affected by biennial yielding. Cultivars had a strong relation to yield — ‘Gita’ (Vf), ‘Konfetnoye’ and ‘Kovalenkovskoye’ had less fluctuation yearly, while ‘White Transparent’, ‘Rubin’ (Kazah.), ‘Antei’, and ‘Ligol’ performed opposite. There was a positive influence observed on reduction of periodicity by mechanical flower thinning, however, there is a discussion on cumulative yield reduction.
大多数苹果品种倾向于两年生,即一年果实多,次年果实少,或者不具有自疏的性质。化学稀释的效果取决于使用时的条件,特别是温度。在其他一些果区,通过改变机构的移动速度和旋转速度,对某些品种进行部分疏花,从而适应机械疏花。本研究的目的是评价在种植后第8 ~ 12年的整个生产期内,在树行一侧和次年另一侧机械除花的效果,以获得正常产量。对7个品种在矮化砧木上的表现进行了比较。矮化砧木B.396上的苹果树受二年生产量的影响较小。品种与产量的关系较强,‘Gita’、‘Konfetnoye’和‘kovalkovskoye’的年波动较小,而‘White Transparent’、‘Rubin’(Kazah.)、‘Antei’和‘Ligol’的年波动相反。机械疏花对减少周期性有积极影响,但对累积产量的减少有讨论。
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引用次数: 0
Integrated Assessment of Oregano (Origanum Vulgare L.) Accessions from the ex situ Collection of Genetic Resources 牛至(Origanum Vulgare L.)的综合评价遗传资源迁地馆藏资料
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0070
I. Sivicka, A. Adamovics, O. Sokolova, G. Lācis, Baiba Krivmane
Abstract Oregano (Origanum vulgare L.) is one of the priority medicinal and aromatic plants in Europe because of its broad potential for use and distribution assessments of the species. The involvement of oregano genetic resources in cultivation can solve the problems of depletion of natural habitats and allows receiving high yield in marginal meteorological conditions. Also, ex situ conservation is the most applicable method for conserving local species of medicinal and aromatic plants in Nordic Europe. There is limited information about the characterisation and evaluation of oregano genetic resources in Latvia and Europe. In this study, long-term observations on 44 oregano accessions maintained at the ex situ collection of medicinal and aromatic plants of the Latvia University of Life Sciences and Technologies, were characterised by the Draft Descriptor List under the agro-climatic conditions of Latvia. Integrated assessment of oregano genetic resources was made to recommend the most valuable accessions for growing.
牛至(Origanum vulgare L.)因其广泛的利用潜力和分布评估而成为欧洲优先药用和芳香植物之一。牛至遗传资源参与种植可以解决自然栖息地枯竭的问题,并可以在边际气象条件下获得高产。此外,移地保护是北欧地区药用和芳香植物本地物种最适用的保护方法。关于拉脱维亚和欧洲牛至遗传资源的特征和评价的信息有限。在这项研究中,对拉脱维亚生命科学与技术大学药用和芳香植物移地收集的44种牛至植物进行了长期观察,并根据拉脱维亚农业气候条件下的描述符清单草案进行了描述。对牛至遗传资源进行综合评价,推荐最有价值的种植材料。
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引用次数: 0
Mixing Hydrogel Granules into Peat Substrate Improves Germination and Enhances Growth and Physiological Performance of Tomato Seedlings 在泥炭基质中掺入水凝胶颗粒可以提高番茄幼苗的萌发率,提高幼苗的生长和生理性能
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0083
A. Balliu, Erjald Haxhiu, G. Sallaku
Abstract The effects of hydrogel granules mixed with peat substrate on germination, morphological and physiological parameters of plugged seedlings were tested in tomato seedlings, at two different irrigation regimes. Due to hydrogel presence, the germination speed was increased, whereas mean germination time was significantly reduced. Slight improvement was recorded regarding final germination, but no effect was found regarding the synchronisation index and seedling vigour index. Due to addition of hydrogel granules a significant increase occurred in dry matter of shoots and plants. No effect was found regarding dry matter of roots, but the presence of hydrogel granules significantly reduced the length of tap root. Interestingly, stomata conductance and chlorophyll index were higher than in control plants. No statistically significant interaction was found between hydrogel variants and irrigation frequency, i.e., the effect of different levels of hydrogel variants did not depend on irrigation frequency. Therefore, we recommend mixing hydrogel capsules into the substrate of plug seedlings to improve the morphological and physiological parameters of seedlings and reduce water and fertiliser leaching into the environment.
摘要以番茄为试验材料,研究了水凝胶颗粒与泥炭基质混合对灌水苗萌发、形态和生理参数的影响。由于水凝胶的存在,萌发速度加快,而平均萌发时间显著缩短。最终发芽率略有提高,但对同步指数和幼苗活力指数没有影响。由于水凝胶颗粒的添加,茎和植物的干物质显著增加。对根的干物质没有影响,但水凝胶颗粒的存在显著减少了根的长度。气孔导度和叶绿素指数均高于对照植株。水凝胶变异与灌溉频率之间没有统计学上显著的交互作用,即不同水平的水凝胶变异的影响不依赖于灌溉频率。因此,我们建议将水凝胶胶囊混合到栓苗的基质中,以改善幼苗的形态和生理参数,减少水和肥料淋失到环境中。
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引用次数: 1
Frost Resistance Potential of Hybrid Apple Seedlings of Russian Research Institute of Fruit Crop Breeding after Artificial Thawing 俄罗斯水果作物育种研究所杂交苹果苗人工解冻后的抗冻潜力
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0078
Z. Ozherelieva, E. Sedov
Abstract The goal of our work was to assess the ability of hybrid apple seedlings and their parent forms to maintain frost resistance during the thaw period, by using artificial freezing methods. Apple resistance was studied in the laboratory by modelling the damage factors of the frost resistance component III with the use of a climatic chamber. All of the studied hybrid apple seedlings from all used crossing combinations showed a sufficient level of frost resistance under modelling of a three-day artificial thaw (+2 °C) with subsequent temperature lowering to –25 °C. The damage of bark, cambium and wood in all of the studied hybrid seedlings was reversible and did not exceed a 2.0 point rating on a 5-point scale. The temperature rise up to +2 °C during three days in February did not cause the activation of growth processes in buds and bark and did not dramatically affect hybrid apple seedlings. According to the results of the artificial freezing, hybrid apple seedlings of the 6026 family (‘Imrus’ × ‘Krasa Sverdlovska’) and hybrids of the 6030 family (‘Svezhest’ × ‘Pepin Orlovsky’) showed the largest potential frost resistance after a thaw in winter.
摘要本研究旨在通过人工冷冻的方法,研究苹果杂交苗木及其亲本在解冻期的抗冻能力。通过使用气候室模拟抗冻性成分III的破坏因素,在实验室中研究了苹果的抗冻性。在为期三天的人工解冻(+2°C)和随后的温度降至-25°C的模拟下,所有被研究的杂交苹果幼苗都表现出足够的抗冻性。在所有研究的杂交幼苗中,树皮、形成层和木材的损害都是可逆的,并且在5分制中不超过2.0分。2月3天温度升高至+2℃,未引起芽和树皮生长过程的激活,对杂交苹果幼苗影响不显著。根据人工冷冻的结果,6026家族(“Imrus”דKrasa Sverdlovska”)和6030家族(“Svezhest”דPepin Orlovsky”)杂交的苹果幼苗在冬季解冻后表现出最大的抗冻潜力。
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引用次数: 0
Goji Berry (Lycİum spp.) Cultivation in Turkey 枸杞(Lycİum属)在土耳其的栽培
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0064
İ. Oğuz, H. I. Oğuz, Ayşen Alay Vural, N. Kafkas
Abstract The goji berry (Lycium spp.) belongs to the Solanaceae family. The genus Lycium comprises 87 recognised species and is distributed in arid and semi-arid regions in temperate to subtropical zones in the world. In botanical terms, there are three types of Lycium species known as wolf-berry and commercially grown in the whole world: Lycium barbarum L. (2n = 24), Lycium chinense Miller (n = 12), and Lycium ruthenicum Murray (n = 12). Wolfberry is a herb growing naturally in both the mild and tropical areas of East, Southeast Asia, and Southern Africa. Also known as wolfberry (Lycium spp.), the motherland of wolfberry is China and it can even be grown in the Himalayas, the highest points of Tibet and Mongolia. Recently, in Turkey goji berry became a popular fruit due to its health benefit compounds, especially phenolic compounds (phenolic acids and flavonoids), carotenoids, tocopherol, ascorbic acid and antioxidant properties. The purpose of this study was to conduct a historical assessment of the botanic properties and cultivation of wolfberry plant and to provide information on the types and cultivars grown commonly in Turkey, distribution areas of production, consumption and use as a technologic product, marketing status, benefits for human health, multiplication and production techniques.
枸杞(Lycium spp.)属于茄科。枸杞属共有87种,分布在温带至亚热带的干旱和半干旱地区。在植物学上,有三种类型的枸杞被称为狼莓,在全世界都有商业种植:枸杞(2n = 24),枸杞(n = 12)和枸杞(n = 12)。枸杞是一种天然生长在东亚、东南亚和非洲南部的温带和热带地区的草本植物。枸杞也被称为枸杞(Lycium spp.),它的祖国是中国,它甚至可以生长在喜马拉雅山,西藏和蒙古的最高点。最近,在土耳其,枸杞因其有益健康的化合物而成为一种受欢迎的水果,特别是酚类化合物(酚酸和类黄酮)、类胡萝卜素、生育酚、抗坏血酸和抗氧化特性。本研究的目的是对枸杞植物的植物学特性和栽培进行历史评估,并提供有关土耳其常见种植的类型和品种、生产分布区域、作为技术产品的消费和使用、市场状况、对人类健康的益处、繁殖和生产技术的信息。
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引用次数: 0
Current Achievements of the Latvian Apple Breeding Programme 拉脱维亚苹果育种计划的最新成果
Q4 Multidisciplinary Pub Date : 2022-08-01 DOI: 10.2478/prolas-2022-0066
L. Ikase, I. Drudze, G. Lācis
Abstract The commercial apple growing industry in Latvia has a lack of climate adapted modern cultivars. The Latvian apple breeding programme is targeted at cultivars adapted to the cool climate of Baltic countries and Scandinavia, with fruit quality and storage suitable for commercial production. Since 1989, scab resistance (Rvi6, Rvi5) donors are included in crosses. Several cultivars already show commercial promise — ‘Dace’, ‘Gita’, ’Monta’ (Rvi6) and the polygenic resistant ‘Laila’ and ‘Pure Ametist’. However, in 2020, the Rvi6 resistance was overcome in Latvia. Donors of polygenic scab tolerance may prove a better alternative. The most successful donors have been ‘Iedzēnu’, ‘Eksotika’, ‘Alesya’, and ‘Bohemia’. Marker assisted gene pyramiding was started, including markers linked to the resistance genes Rvi5 and Rvi6. Scab resistant cultivars ‘Edite’, ‘Felicita’, ‘Ligita’, and ‘Paulis’ show low susceptibility to Neonectria ditissima canker. To achieve high fruit quality and good storage, worldwide commercial cultivars are crossed with local adapted cultivars. Such crosses that have been registered include ‘Inta’, ‘Lora’, and ‘Saulesmeita’. Recent crosses for quality include ‘Honeycrisp’, where H-1-07-36 and H-1-07-42 show most promise. Improved biochemical content and flesh structure is one of the main targets. An additional line of breeding is aimed at ornamental and cider apples. The scab resistant Nr.19-97-154 (Cidross) was selected for cider. The red leaf columnar apple ‘Karlens’ and the compact red-leaf crab ‘Auce’ have been registered.
拉脱维亚的商业苹果种植业缺乏适应气候的现代品种。拉脱维亚苹果育种计划的目标是培育适应波罗的海国家和斯堪的纳维亚半岛凉爽气候的品种,其果实质量和储存适合商业生产。自1989年以来,抗痂病(Rvi6, Rvi5)供体被纳入杂交。几个品种已经显示出商业前景——“Dace”、“Gita”、“Monta”(Rvi6)以及多基因抗性的“Laila”和“Pure Ametist”。然而,在2020年,拉脱维亚克服了对Rvi6的抵抗。多基因痂耐受性的供体可能是更好的选择。最成功的捐赠者是“Iedzēnu”、“Eksotika”、“Alesya”和“Bohemia”。开始了标记辅助基因金字塔,包括与抗性基因Rvi5和Rvi6相关的标记。抗痂病品种“Edite”、“Felicita”、“Ligita”和“Paulis”对新ectria ditissima canker的敏感性较低。为了获得高品质的果实和良好的储藏,世界各地的商品品种与当地的适应品种杂交。已登记的这类十字架包括“Inta”、“Lora”和“Saulesmeita”。最近的优质杂交品种包括“蜜脆”,其中H-1-07-36和H-1-07-42最有希望。改善生物化学含量和果肉结构是主要目标之一。另一个育种系是针对观赏苹果和苹果酒。选择抗结痂的Nr.19-97-154 (Cidross)作为苹果酒品种。红叶柱状苹果“Karlens”和紧凑的红叶蟹“Auce”已经注册。
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引用次数: 4
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Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.
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