Pub Date : 2023-04-01DOI: 10.2478/prolas-2023-0020
E. Berga-Švītiņa, V. Pirsko, M. Nakazawa-Miklaševiča, J. Maksimenko, J. Gardovskis, E. Miklaševičs
Abstract Germline pathogenic BRCA1 variants confer increased risk of breast and/or ovarian cancer. The penetrance of BRCA1 pathogenic variants is variable due to the effects of other genetic factors. The interaction between CHEK2 and BRCA1 proteins is crucial in homology directed DNA repair pathway. The aim of this study was to assess the effect of three pathogenic/likely pathogenic variants of the CHEK2 gene on BRCA1 pathogenic allelic variant penetrance. The analysis included 380 DNA samples of women with confirmed positive BRCA1 status for one of founder variants c.4035del and c.5266dup. The c.444+1G>A and c.470T>C variants of CHEK2 gene were identified by Sanger’s sequencing, and the del5395 variant was detected by multiplex PCR. The studied CHEK2 variants were found in 13 double heterozygous cases (c.444+1G>A, n = 1; c.470T>C, n = 11, del5395, n = 1). Although the prevalence of CHEK2 variants in the ovarian cancer group was comparatively high (5.41%), the increase of the ovarian cancer risk was not statistically significant (OR = 1.56; 95% CI: 0.32–9.94; p = 0.73). The association of the age at the onset of cancer with the presence of particular CHEK2 variant was not consistent.
种系致病性BRCA1变异会增加乳腺癌和/或卵巢癌的风险。由于其他遗传因素的影响，BRCA1致病变异的外显率是可变的。CHEK2和BRCA1蛋白之间的相互作用在同源定向DNA修复途径中至关重要。本研究的目的是评估CHEK2基因的三种致病/可能致病变异对BRCA1致病等位基因变异外显率的影响。该分析包括380名女性的DNA样本，这些女性的BRCA1基因被证实为c.4035del和c.5266dup的创始变异之一。Sanger测序法鉴定CHEK2基因的C .444+1G>A和C . 470t >C变异，多重PCR检测CHEK2基因的del5395变异。所研究的CHEK2变异在13例双杂合病例中发现(c.444+1G>A, n = 1;C . 470t >C, n = 11, del5395, n = 1)。虽然CHEK2变异在卵巢癌组中患病率较高(5.41%)，但卵巢癌风险增加无统计学意义(OR = 1.56;95% ci: 0.32-9.94;P = 0.73)。癌症发病年龄与特定CHEK2变异存在的关系并不一致。
{"title":"Penetrance of CHEK2 and BRCA1 Double Heterozygotes in Breast and/or Ovarian Cancer Patients","authors":"E. Berga-Švītiņa, V. Pirsko, M. Nakazawa-Miklaševiča, J. Maksimenko, J. Gardovskis, E. Miklaševičs","doi":"10.2478/prolas-2023-0020","DOIUrl":"https://doi.org/10.2478/prolas-2023-0020","url":null,"abstract":"Abstract Germline pathogenic BRCA1 variants confer increased risk of breast and/or ovarian cancer. The penetrance of BRCA1 pathogenic variants is variable due to the effects of other genetic factors. The interaction between CHEK2 and BRCA1 proteins is crucial in homology directed DNA repair pathway. The aim of this study was to assess the effect of three pathogenic/likely pathogenic variants of the CHEK2 gene on BRCA1 pathogenic allelic variant penetrance. The analysis included 380 DNA samples of women with confirmed positive BRCA1 status for one of founder variants c.4035del and c.5266dup. The c.444+1G>A and c.470T>C variants of CHEK2 gene were identified by Sanger’s sequencing, and the del5395 variant was detected by multiplex PCR. The studied CHEK2 variants were found in 13 double heterozygous cases (c.444+1G>A, n = 1; c.470T>C, n = 11, del5395, n = 1). Although the prevalence of CHEK2 variants in the ovarian cancer group was comparatively high (5.41%), the increase of the ovarian cancer risk was not statistically significant (OR = 1.56; 95% CI: 0.32–9.94; p = 0.73). The association of the age at the onset of cancer with the presence of particular CHEK2 variant was not consistent.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81871041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.2478/prolas-2023-0015
L. Plavina, Silvija Umbraško, Lāsma Asare, Edgars Edelmers
Abstract Specific high physical and psychological load energy expenditure should be covered by balanced diet that is adapted to physical load. Food intake is one of the vital processes that support body activity and maintain physical working capacity in special environment. Various objective and subjective factors have an impact on body energy expenditure and determine a definite amount of food energy. Dietary intake influences the readiness and training performance outcome. The purpose of the study was to evaluate the diet of physically active persons before and after a nutritional education course and determine the impact of nutrition knowledge on nutrition behaviour and diet in two subgroups: respondents with a standard BMI level (BMI < 25, in the interval 18.5–24.9) and overweight respondents with BMI ³ 25, in the interval 25.0–29.9. Participants aged 22–35 years, who had daily physical activity and physical load, were selected for the study group. We divided respondents into two subgroups: respondents with standard BMI level (BMI < 25; BMI in the interval 18.5–24.9) and overweight respondents with BMI ³ 25 (BMI in the interval 25.0–29.9). Nutritional education course included the theoretical part (lectures) and practical part (dietary diary self-assessment) as well as a quiz that allow to evaluate nutrition knowledge level in the selected cohort of respondents with BMI < 25 and BMI ³ 25. We provided intervention before and after a nutritional education course by using the standardised questionnaire “Diet 3-day menu diary” and standardised survey “Physical activity during the current life period”, which allowed to evaluate the balance between the daily intake for energy recovery and daily physical activity as energy expenditure. We determined the value of the main dietary components (protein (%), carbohydrates (%) and fat (%), as well the total amount of energy (kcal) in the diet before the nutritional education course (Diet 1st) and after the nutritional education course (Diet 2nd) in the selected cohort of respondents with BMI < 25 and BMI ³ 25. The study group participants preferred a diet with a higher amount of fat and lower amount of carbohydrates compared with nutritional recommendation for general population. There were no significant differences in Diet 1st components between overweight and standard BMI groups. Analysis of post-course (Diet 2nd) dietary diary showed a statistically exact significance of fat level (%) and carbohydrate level (%), and no statistically approved changes in protein level (%) intake. Nutritional knowledge of the study group participants after the nutritional education course was evaluated by using a standardised test in points (1–10), which showed that about 60% of the respondents received an assessment “good”. The results of the study can be used to develop optimal diet planning during the pre-training period before planning physical exercises with high physical and psycho-emotional load, in order to benef
{"title":"Evaluation of the Impact of Nutrition Knowledge on Nutrition Behaviour and Diet in a Physically Active Person’s Cohort","authors":"L. Plavina, Silvija Umbraško, Lāsma Asare, Edgars Edelmers","doi":"10.2478/prolas-2023-0015","DOIUrl":"https://doi.org/10.2478/prolas-2023-0015","url":null,"abstract":"Abstract Specific high physical and psychological load energy expenditure should be covered by balanced diet that is adapted to physical load. Food intake is one of the vital processes that support body activity and maintain physical working capacity in special environment. Various objective and subjective factors have an impact on body energy expenditure and determine a definite amount of food energy. Dietary intake influences the readiness and training performance outcome. The purpose of the study was to evaluate the diet of physically active persons before and after a nutritional education course and determine the impact of nutrition knowledge on nutrition behaviour and diet in two subgroups: respondents with a standard BMI level (BMI < 25, in the interval 18.5–24.9) and overweight respondents with BMI ³ 25, in the interval 25.0–29.9. Participants aged 22–35 years, who had daily physical activity and physical load, were selected for the study group. We divided respondents into two subgroups: respondents with standard BMI level (BMI < 25; BMI in the interval 18.5–24.9) and overweight respondents with BMI ³ 25 (BMI in the interval 25.0–29.9). Nutritional education course included the theoretical part (lectures) and practical part (dietary diary self-assessment) as well as a quiz that allow to evaluate nutrition knowledge level in the selected cohort of respondents with BMI < 25 and BMI ³ 25. We provided intervention before and after a nutritional education course by using the standardised questionnaire “Diet 3-day menu diary” and standardised survey “Physical activity during the current life period”, which allowed to evaluate the balance between the daily intake for energy recovery and daily physical activity as energy expenditure. We determined the value of the main dietary components (protein (%), carbohydrates (%) and fat (%), as well the total amount of energy (kcal) in the diet before the nutritional education course (Diet 1st) and after the nutritional education course (Diet 2nd) in the selected cohort of respondents with BMI < 25 and BMI ³ 25. The study group participants preferred a diet with a higher amount of fat and lower amount of carbohydrates compared with nutritional recommendation for general population. There were no significant differences in Diet 1st components between overweight and standard BMI groups. Analysis of post-course (Diet 2nd) dietary diary showed a statistically exact significance of fat level (%) and carbohydrate level (%), and no statistically approved changes in protein level (%) intake. Nutritional knowledge of the study group participants after the nutritional education course was evaluated by using a standardised test in points (1–10), which showed that about 60% of the respondents received an assessment “good”. The results of the study can be used to develop optimal diet planning during the pre-training period before planning physical exercises with high physical and psycho-emotional load, in order to benef","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88507051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.2478/prolas-2023-0019
R. Fadhil, D. Nurba, F. Fachruddin
Abstract Post-harvest handling of coffee is one of the main factors in producing a high-quality coffee, including its processing techniques. This study aimed to conduct a sensory evaluation of arabica Gayo coffee flavour in various varieties and processing techniques. There were four selected coffee varieties in this study, namely Bor-bor, Tim Tim Aceh, Bergendal and Ateng Super, which were obtained from the garden of farmers in Gayo Highlands, Province of Aceh, Indonesia. The coffee processing methods used in this study were both dry and wet processing techniques. The sensory assessment of arabica Gayo coffee flavour was carried out by ten panelists by evaluating the acceptance of the product based on the level of preference. The sensory assessment of flavour included fragrance, flavour, after-taste, acidity, balance, body, and overall based on the panelist decision values using the exponential comparison method (ECM/MPE). Results showed that the wet processing technique was the most suitable technique for arabica Gayo coffee based on the preference of panelists compared to the dry processing technique. Arabica Gayo coffee varieties that have the highest acceptance value by panelists in sequence were Bor-bor varieties (1st choice), Tim Tim Aceh (2nd choice), Ateng Super (3rd choice), and Bergendal (4th choice). Therefore, the Bor-bor variety was the most important coffee variety recommended to be developed by the farmers compared to other coffee varieties.
咖啡收获后的处理是生产高品质咖啡的主要因素之一，包括其加工技术。本研究旨在对阿拉比卡加约咖啡在不同品种和加工工艺下的风味进行感官评价。本研究选择了四个咖啡品种，分别是Bor-bor, Tim Aceh, Bergendal和Ateng Super，它们来自印度尼西亚亚齐省Gayo高地的农民花园。本研究中使用的咖啡加工方法有干法和湿法两种。阿拉比卡加约咖啡风味的感官评估是由10名小组成员根据偏好程度评估产品的接受程度进行的。风味的感官评价包括香味、风味、余味、酸度、平衡、体度和总体评价，采用指数比比法(ECM/MPE)。结果表明，与干法加工技术相比，基于小组成员的偏好，湿法加工技术是最适合阿拉比卡加约咖啡的技术。小组成员接受价值最高的阿拉比卡加约咖啡品种依次为Bor-bor品种(第一选择)，Tim Tim Aceh(第二选择)，Ateng Super(第三选择)和Bergendal(第四选择)。因此，与其他咖啡品种相比，Bor-bor品种是农民推荐开发的最重要的咖啡品种。
{"title":"A Sensory Evaluation of Arabica Gayo Coffee Flavour Based on Varieties and Processing Techniques","authors":"R. Fadhil, D. Nurba, F. Fachruddin","doi":"10.2478/prolas-2023-0019","DOIUrl":"https://doi.org/10.2478/prolas-2023-0019","url":null,"abstract":"Abstract Post-harvest handling of coffee is one of the main factors in producing a high-quality coffee, including its processing techniques. This study aimed to conduct a sensory evaluation of arabica Gayo coffee flavour in various varieties and processing techniques. There were four selected coffee varieties in this study, namely Bor-bor, Tim Tim Aceh, Bergendal and Ateng Super, which were obtained from the garden of farmers in Gayo Highlands, Province of Aceh, Indonesia. The coffee processing methods used in this study were both dry and wet processing techniques. The sensory assessment of arabica Gayo coffee flavour was carried out by ten panelists by evaluating the acceptance of the product based on the level of preference. The sensory assessment of flavour included fragrance, flavour, after-taste, acidity, balance, body, and overall based on the panelist decision values using the exponential comparison method (ECM/MPE). Results showed that the wet processing technique was the most suitable technique for arabica Gayo coffee based on the preference of panelists compared to the dry processing technique. Arabica Gayo coffee varieties that have the highest acceptance value by panelists in sequence were Bor-bor varieties (1st choice), Tim Tim Aceh (2nd choice), Ateng Super (3rd choice), and Bergendal (4th choice). Therefore, the Bor-bor variety was the most important coffee variety recommended to be developed by the farmers compared to other coffee varieties.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79462477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.2478/prolas-2023-0012
N. Sydorova
Abstract The article presents data on the most commonly used antiarrhythmic drugs of subclass IC: propafenone, flecainide, and a drug manufactured in Latvia — ethacizine. The features and priority areas of each drug use were identified. The author conducted a critical analysis of the results of Cardiac Arrhythmia Suppression Trials (CAST)-I and CAST-II and suggested ways to further study of subclass IC antiarrhythmic drugs in the context of current treatment strategies. The results of CAST-I and CAST-II should be interpreted in view of the heterogeneity of the subclass IC antiarrhythmic drugs and the fact that among the drugs of this subclass that are currently registered and approved for use in Ukraine, only flecainide was studied in CAST-I, and none of these drugs — in CAST-II. Ethacizine has a special position as a promising drug, especially effective in the treatment of vagus-induced heart rhythm disorders, with a small number of contraindications and drug interactions compared with propafenone and flecainide. Despite many years of use in several countries, this drug remains “invisible” to researchers around the world due to a limited number of international studies. The author draws attention to the need to re-evaluate antiarrhythmic drugs with an outdated evidence, in some cases quite limited, and to plan new experimental studies to clarify the possibility of subclass IC drugs to influence new pharmacological targets in arrhythmology, as well as large-scale well-planned studies of the safety and efficacy of subclass IC antiarrhythmic drugs in real present-day clinical practice.
{"title":"Class IC Antiarrhythmic Drugs: Informed Choice","authors":"N. Sydorova","doi":"10.2478/prolas-2023-0012","DOIUrl":"https://doi.org/10.2478/prolas-2023-0012","url":null,"abstract":"Abstract The article presents data on the most commonly used antiarrhythmic drugs of subclass IC: propafenone, flecainide, and a drug manufactured in Latvia — ethacizine. The features and priority areas of each drug use were identified. The author conducted a critical analysis of the results of Cardiac Arrhythmia Suppression Trials (CAST)-I and CAST-II and suggested ways to further study of subclass IC antiarrhythmic drugs in the context of current treatment strategies. The results of CAST-I and CAST-II should be interpreted in view of the heterogeneity of the subclass IC antiarrhythmic drugs and the fact that among the drugs of this subclass that are currently registered and approved for use in Ukraine, only flecainide was studied in CAST-I, and none of these drugs — in CAST-II. Ethacizine has a special position as a promising drug, especially effective in the treatment of vagus-induced heart rhythm disorders, with a small number of contraindications and drug interactions compared with propafenone and flecainide. Despite many years of use in several countries, this drug remains “invisible” to researchers around the world due to a limited number of international studies. The author draws attention to the need to re-evaluate antiarrhythmic drugs with an outdated evidence, in some cases quite limited, and to plan new experimental studies to clarify the possibility of subclass IC drugs to influence new pharmacological targets in arrhythmology, as well as large-scale well-planned studies of the safety and efficacy of subclass IC antiarrhythmic drugs in real present-day clinical practice.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83065071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.2478/prolas-2023-0005
Niks Ričards Goldiņš, K. Meidrops, Lauma Apine, E. Petrošina, P. Stradins, V. Groma
Abstract Infective endocarditis is a disease that affects the endocardium and often alters heart valves, notably the aortic valve. Bacteraemia and valvular endothelial damage play an essential role in the pathogenesis of infective endocarditis. The pertinent literature suggests that neutrophil extracellular traps are important contributors to the development of the disease. However, features of the valvular damage and contribution of neutrophils to the alteration of cardiac tissue are not explored sufficiently. The purpose of this study was to investigate the occurrence and distribution of neutrophilic leukocytes and neutrophil extracellular traps in native aortic valves affected by infective endocarditis, using histopathology and immunohistochemistry assays. In addition, the presence of vegetations on the heart valve was determined. Infiltration of neutrophils into the valvular leaflet was significantly more severe at the free margin (mean 5.89 ± 3.00, p < 0.001) and the middle portion (mean 4.58 ± 3.64, p = 0.032) when compared to the base portion (2.05 ± 1.90). No significant differences in neutrophilic leukocyte infiltrating inflammatory lesions were found between cusp layers. The presence of myeloperoxidase and citrullinated histone expression characteristic of neutrophil extracellular traps was demonstrated by the use of immunohistochemistry in IE-affected valvular leaflets and vegetations. Collectively, the study results suggest that the free cusp margin and its middle portion of the aortic valve are exposed to enforced blood flow; endothelial damage and vegetation formation are likely to occur along with the presence of infective endocarditis-related bacteraemia.
{"title":"Neutrophilic Leukocytes and Neutrophil Extracellular Traps in Native Aortic Valve Endocarditis","authors":"Niks Ričards Goldiņš, K. Meidrops, Lauma Apine, E. Petrošina, P. Stradins, V. Groma","doi":"10.2478/prolas-2023-0005","DOIUrl":"https://doi.org/10.2478/prolas-2023-0005","url":null,"abstract":"Abstract Infective endocarditis is a disease that affects the endocardium and often alters heart valves, notably the aortic valve. Bacteraemia and valvular endothelial damage play an essential role in the pathogenesis of infective endocarditis. The pertinent literature suggests that neutrophil extracellular traps are important contributors to the development of the disease. However, features of the valvular damage and contribution of neutrophils to the alteration of cardiac tissue are not explored sufficiently. The purpose of this study was to investigate the occurrence and distribution of neutrophilic leukocytes and neutrophil extracellular traps in native aortic valves affected by infective endocarditis, using histopathology and immunohistochemistry assays. In addition, the presence of vegetations on the heart valve was determined. Infiltration of neutrophils into the valvular leaflet was significantly more severe at the free margin (mean 5.89 ± 3.00, p < 0.001) and the middle portion (mean 4.58 ± 3.64, p = 0.032) when compared to the base portion (2.05 ± 1.90). No significant differences in neutrophilic leukocyte infiltrating inflammatory lesions were found between cusp layers. The presence of myeloperoxidase and citrullinated histone expression characteristic of neutrophil extracellular traps was demonstrated by the use of immunohistochemistry in IE-affected valvular leaflets and vegetations. Collectively, the study results suggest that the free cusp margin and its middle portion of the aortic valve are exposed to enforced blood flow; endothelial damage and vegetation formation are likely to occur along with the presence of infective endocarditis-related bacteraemia.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79128034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.2478/prolas-2023-0007
A. Požarskis, Rita Požarska, Ļubova Baranovska
Abstract This study seeks to determine whether patients from a family physician’s practice have an association between 25(OH)D levels and primary arterial hypertension (AH). The study included a total of 1068 patients who were tested for vitamin D status. Data from their outpatient medical records were analysed: sex, age, body mass index, glomerular filtration rate, and the history of AH. Primary arterial hypertension was diagnosed in 63% of the patients. The mean vitamin D level in the study population was 25 ng/ml, and the largest group, or 36%, was found to have vitamin D deficiency (20–29 ng/ml). Odds ratio (OR) for hypertension was not inversely correlated with higher vitamin D levels. No statistically significant increase was observed in OR in a multi-factor analysis. The relevant hypertension ORs were 1.8 (0.4–7.5), 1.1 (0.3–4.5), 1.7 (0.4–7.2) and 0.7 (0.1–4.8) 30 ng/ml to 45 ng/ml, 20 to 29 ng/ml, 10 to 19 ng/ml, and < 10 ng/ml compared to the group of ³ 45 ng/ml. No association was found between 25(OH)D levels and the primary arterial hypertension in study participants.
{"title":"Association Between 25(OH)D Levels and Primary Arterial Hypertension","authors":"A. Požarskis, Rita Požarska, Ļubova Baranovska","doi":"10.2478/prolas-2023-0007","DOIUrl":"https://doi.org/10.2478/prolas-2023-0007","url":null,"abstract":"Abstract This study seeks to determine whether patients from a family physician’s practice have an association between 25(OH)D levels and primary arterial hypertension (AH). The study included a total of 1068 patients who were tested for vitamin D status. Data from their outpatient medical records were analysed: sex, age, body mass index, glomerular filtration rate, and the history of AH. Primary arterial hypertension was diagnosed in 63% of the patients. The mean vitamin D level in the study population was 25 ng/ml, and the largest group, or 36%, was found to have vitamin D deficiency (20–29 ng/ml). Odds ratio (OR) for hypertension was not inversely correlated with higher vitamin D levels. No statistically significant increase was observed in OR in a multi-factor analysis. The relevant hypertension ORs were 1.8 (0.4–7.5), 1.1 (0.3–4.5), 1.7 (0.4–7.2) and 0.7 (0.1–4.8) 30 ng/ml to 45 ng/ml, 20 to 29 ng/ml, 10 to 19 ng/ml, and < 10 ng/ml compared to the group of ³ 45 ng/ml. No association was found between 25(OH)D levels and the primary arterial hypertension in study participants.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91087799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.2478/prolas-2023-0003
J. Zepa, V. Lavrentjevs, Santa Miķēna, J. Arajs, Tamāra Zavgorodnaja, E. Sikora, Sarmīte Ābelīte, Renāte Diura, I. Balčune, K. Ivanova, Pauls Rubīns, I. Trapina, D. Andersone, I. Buliņa
Abstract The aim of this study was to analyse the rheumatic disease profile and treatment aspects of the patients consulted in the outpatient department of Pauls Stradiņš Clinical University Hospital during the first month of the COVID-19 pandemic from 2020 March 13 till April 14. A total of 457 (76.04%) remote and 144 (23.96%) face-to-face consultations were analysed, totalling 601 patients: 434 (72.21%) females and 167 (27.79%) males with mean age 51.40 ± 14.73 years. Rheumatoid arthritis (223 (37.10%)), psoriatic arthritis (93 (15.47%)) and ankylosing spondylitis (80 (13.31%)) were the most frequently consulted conditions. Disease modifying antirheumatic drugs (DMARDs) or immunosuppressants (IS) were taken by 515 (85.69%) patients. These included synthetic DMARD (242 (46.99%)), mainly methotrexate; and biologic DMARD (156 (30.29%)), mainly tumour necrosis factor inhibitor. More than one-half of the cohort (427 (71.05%)) was not taking a glucocorticoid (GC). NSAIDs were used in 391 (65.08%) patients, mainly on demand (354 (90.54%)). Most patients (401 (66.72%)) had no comorbidities (hypertension, diabetes, malignancy and/or chronic respiratory disease). The profile of patients consulted in the outpatient department consisted mainly of middle-age females with autoimmune inflammatory arthritis treated by DMARD. Most of the patients did not use GCs, they did not regularly use NSAIDs and did not have comorbidities. Telemedicine is an acceptable way of care delivery for chronic rheumatic patients with previously known disease and treatment, especially during a pandemic.
{"title":"Outpatient Care Aspects of Rheumatic Patients in Latvia: Real Life Data in the Context of the First Month of the COVID-19 Pandemic","authors":"J. Zepa, V. Lavrentjevs, Santa Miķēna, J. Arajs, Tamāra Zavgorodnaja, E. Sikora, Sarmīte Ābelīte, Renāte Diura, I. Balčune, K. Ivanova, Pauls Rubīns, I. Trapina, D. Andersone, I. Buliņa","doi":"10.2478/prolas-2023-0003","DOIUrl":"https://doi.org/10.2478/prolas-2023-0003","url":null,"abstract":"Abstract The aim of this study was to analyse the rheumatic disease profile and treatment aspects of the patients consulted in the outpatient department of Pauls Stradiņš Clinical University Hospital during the first month of the COVID-19 pandemic from 2020 March 13 till April 14. A total of 457 (76.04%) remote and 144 (23.96%) face-to-face consultations were analysed, totalling 601 patients: 434 (72.21%) females and 167 (27.79%) males with mean age 51.40 ± 14.73 years. Rheumatoid arthritis (223 (37.10%)), psoriatic arthritis (93 (15.47%)) and ankylosing spondylitis (80 (13.31%)) were the most frequently consulted conditions. Disease modifying antirheumatic drugs (DMARDs) or immunosuppressants (IS) were taken by 515 (85.69%) patients. These included synthetic DMARD (242 (46.99%)), mainly methotrexate; and biologic DMARD (156 (30.29%)), mainly tumour necrosis factor inhibitor. More than one-half of the cohort (427 (71.05%)) was not taking a glucocorticoid (GC). NSAIDs were used in 391 (65.08%) patients, mainly on demand (354 (90.54%)). Most patients (401 (66.72%)) had no comorbidities (hypertension, diabetes, malignancy and/or chronic respiratory disease). The profile of patients consulted in the outpatient department consisted mainly of middle-age females with autoimmune inflammatory arthritis treated by DMARD. Most of the patients did not use GCs, they did not regularly use NSAIDs and did not have comorbidities. Telemedicine is an acceptable way of care delivery for chronic rheumatic patients with previously known disease and treatment, especially during a pandemic.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83283597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.2478/prolas-2023-0006
E. Leonova, Kristīne Dišlere, E. Rostoka, L. Baumane, E. Bisenieks, G. Duburs, N. Sjakste
Abstract The goal of the study was to test the ability of a representative of the 1,4-DHP group — glutapyrone to protect DNA against damage. The compound increased the concentration of the hydroxyl radicals produced in the Fenton reaction, it did not scavenge peroxynitrite and did not protect plasmid DNA against damage by products of the Fenton reaction. On the other hand, the compound protected HeLa cells against damage by peroxinitrite and B-lymphocytes against DNA damage in the conditions of oxidative stress induced by transfection of the Tat protein derived from the HIV virus. It is supposed that glutapyrone mobilises DNA repair systems.
{"title":"Study of Impact of a 1,4-DHP Derivative Glutapyrone on DNA Using In Vitro Tests","authors":"E. Leonova, Kristīne Dišlere, E. Rostoka, L. Baumane, E. Bisenieks, G. Duburs, N. Sjakste","doi":"10.2478/prolas-2023-0006","DOIUrl":"https://doi.org/10.2478/prolas-2023-0006","url":null,"abstract":"Abstract The goal of the study was to test the ability of a representative of the 1,4-DHP group — glutapyrone to protect DNA against damage. The compound increased the concentration of the hydroxyl radicals produced in the Fenton reaction, it did not scavenge peroxynitrite and did not protect plasmid DNA against damage by products of the Fenton reaction. On the other hand, the compound protected HeLa cells against damage by peroxinitrite and B-lymphocytes against DNA damage in the conditions of oxidative stress induced by transfection of the Tat protein derived from the HIV virus. It is supposed that glutapyrone mobilises DNA repair systems.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78642089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.2478/prolas-2023-0008
Loreta Briuka, J. Kroiča, Sintija Miluna, I. Skadiņš, Šarlote Agate Vanka, D. Rostoka
Abstract Regarding oral biofilm aspects, there has been strong evidence for a microbiotic component in the aetiology of halitosis. Many oral microbiota have protheolytic and putative activity, but there have been no studies investigating the association of microbiota in oral biofilms with halitosis. The objective of this study was to determine species of oral microbiota in the periodontal area and dorsal part of tongue biofilm, and how their quantitative amounts differ in halitosis patients. The clinical bacterial material from halitosis patients (altogether 98 persons, volatile sulphur compounds (VSC) on average 380 ppb) was taken from periodontal pockets and the dorsal part of the tongue for microbiological diagnostics of anaerobic bacteria, using polymerase chain reaction (PCR) tests for the comparison of bacterial quantity. The study showed the primary aetiology factors of halitosis in Latvia, and offers possible versions of microbiological diagnostics of halitosis. Even though the examination of halitosis patients and determination of VSC using a halimeter is technically simpler and cheaper, the determination of aetiological factors and their combinations using microbiological examination of clinical material with PCR tests are more precise. A characteristic ecological niche of anaerobic bacteria is not only the anaerobic environment of periodontal pockets, but also the microbiota of the dorsal part of the tongue. Additionally, some anaerobic bacteria species (Porohyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia) in larger amounts are found on the microbiome of the tongue. Therefore, it is advisable to begin microbiological diagnostics in halitosis patients with quantitative diagnostics of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola on the biofilm of the tongue coating.
{"title":"Microbiological Examination of Clinical Material in Halitosis Patients","authors":"Loreta Briuka, J. Kroiča, Sintija Miluna, I. Skadiņš, Šarlote Agate Vanka, D. Rostoka","doi":"10.2478/prolas-2023-0008","DOIUrl":"https://doi.org/10.2478/prolas-2023-0008","url":null,"abstract":"Abstract Regarding oral biofilm aspects, there has been strong evidence for a microbiotic component in the aetiology of halitosis. Many oral microbiota have protheolytic and putative activity, but there have been no studies investigating the association of microbiota in oral biofilms with halitosis. The objective of this study was to determine species of oral microbiota in the periodontal area and dorsal part of tongue biofilm, and how their quantitative amounts differ in halitosis patients. The clinical bacterial material from halitosis patients (altogether 98 persons, volatile sulphur compounds (VSC) on average 380 ppb) was taken from periodontal pockets and the dorsal part of the tongue for microbiological diagnostics of anaerobic bacteria, using polymerase chain reaction (PCR) tests for the comparison of bacterial quantity. The study showed the primary aetiology factors of halitosis in Latvia, and offers possible versions of microbiological diagnostics of halitosis. Even though the examination of halitosis patients and determination of VSC using a halimeter is technically simpler and cheaper, the determination of aetiological factors and their combinations using microbiological examination of clinical material with PCR tests are more precise. A characteristic ecological niche of anaerobic bacteria is not only the anaerobic environment of periodontal pockets, but also the microbiota of the dorsal part of the tongue. Additionally, some anaerobic bacteria species (Porohyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia) in larger amounts are found on the microbiome of the tongue. Therefore, it is advisable to begin microbiological diagnostics in halitosis patients with quantitative diagnostics of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola on the biofilm of the tongue coating.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87172477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.2478/prolas-2023-0010
Silvija Umbraško, J. Vētra, L. Plavina, Liene Martinsone-Bērzkalne, I. Duļevska, Edgars Edelmers
Abstract The growth and maturation of a child’s body are going on continuously, but unevenly. Therefore, children of the same age may have different growth and functional abilities. On the initiative of the Latvian government, a pilot project was launched, which aims to evaluate the readiness of children to start school at the age of six as well as compare physical development, separate functional abilities, and posture for six and seven-year-old children of regions of Latvia and Rīga preschool educational institutions. The study involved 918 children, who were divided into two groups — Rīga (R) and regions of Latvia (RL). Respondents in each group were further divided by age — six- and seven-year-olds, and by sex. Anthropometric parameters were determined for each individual’s height, weight, chest circumference, lung vital capacity (PVC), forearm flexor muscle strength, and posture. In our study, the mean values of height for six-year-old girls were: R – 117.6 ± 5.8 cm, RL – 117.1 ± 6.3 cm, for boys R – 118.7 ± 5.0 cm, RL – 118.6 ± 5.1 cm. Seven-year-old children had an average increase in chest circumference of 1 to 2 cm, both by sex and by place of residence. Symmetrical posture was observed only for six-year-old children in 23.1% of cases and 17.1% of seven-year-old children. 59.5% of the children in the study group spent more than one hour a day watching TV, and 66.3% played computer games every day. The results of the study showed that children aged six and seven years grew and functionally developed very differently and individually. These age groups of children did not have accelerated growth ages; there were no large annual increases. A relatively small sex dimorphism was observed. The readiness of six-year-old children to start school should be assessed very individually by the child’s parents in collaboration with the pediatrician.
儿童身体的生长和成熟是连续的，但不是均匀的。因此，相同年龄的儿童可能有不同的生长和功能能力。在拉脱维亚政府的倡议下，启动了一个试点项目，目的是评估儿童在6岁时入学的准备情况，并比较拉脱维亚各地区和塞尔维亚学前教育机构的6岁和7岁儿童的身体发育、不同的功能能力和姿势。这项研究涉及918名儿童，他们被分为两组——里加(R)和拉脱维亚地区(RL)。每组的受访者进一步按年龄(6岁和7岁)和性别划分。确定了每个人的身高、体重、胸围、肺活量(PVC)、前臂屈肌力量和姿势的人体测量参数。在我们的研究中，6岁女孩的平均身高为:R - 117.6±5.8 cm, RL - 117.1±6.3 cm;男孩的平均身高为R - 118.7±5.0 cm, RL - 118.6±5.1 cm。七岁儿童的胸围平均增加1至2厘米，性别和居住地均不同。只有23.1%的6岁儿童和17.1%的7岁儿童有对称的姿势。59.5%的孩子每天看电视超过一个小时，66.3%的孩子每天玩电脑游戏。研究结果表明，六岁和七岁的孩子在成长和功能发展方面有很大的不同，而且各不相同。这些年龄组的儿童没有加速生长年龄;没有大的年度增长。观察到相对较小的性别二态现象。6岁儿童入学的准备情况应该由孩子的父母与儿科医生合作进行非常单独的评估。
{"title":"Physical Development of Six- and Seven-Year-Old Children in Rīga and Latvian Regions","authors":"Silvija Umbraško, J. Vētra, L. Plavina, Liene Martinsone-Bērzkalne, I. Duļevska, Edgars Edelmers","doi":"10.2478/prolas-2023-0010","DOIUrl":"https://doi.org/10.2478/prolas-2023-0010","url":null,"abstract":"Abstract The growth and maturation of a child’s body are going on continuously, but unevenly. Therefore, children of the same age may have different growth and functional abilities. On the initiative of the Latvian government, a pilot project was launched, which aims to evaluate the readiness of children to start school at the age of six as well as compare physical development, separate functional abilities, and posture for six and seven-year-old children of regions of Latvia and Rīga preschool educational institutions. The study involved 918 children, who were divided into two groups — Rīga (R) and regions of Latvia (RL). Respondents in each group were further divided by age — six- and seven-year-olds, and by sex. Anthropometric parameters were determined for each individual’s height, weight, chest circumference, lung vital capacity (PVC), forearm flexor muscle strength, and posture. In our study, the mean values of height for six-year-old girls were: R – 117.6 ± 5.8 cm, RL – 117.1 ± 6.3 cm, for boys R – 118.7 ± 5.0 cm, RL – 118.6 ± 5.1 cm. Seven-year-old children had an average increase in chest circumference of 1 to 2 cm, both by sex and by place of residence. Symmetrical posture was observed only for six-year-old children in 23.1% of cases and 17.1% of seven-year-old children. 59.5% of the children in the study group spent more than one hour a day watching TV, and 66.3% played computer games every day. The results of the study showed that children aged six and seven years grew and functionally developed very differently and individually. These age groups of children did not have accelerated growth ages; there were no large annual increases. A relatively small sex dimorphism was observed. The readiness of six-year-old children to start school should be assessed very individually by the child’s parents in collaboration with the pediatrician.","PeriodicalId":20651,"journal":{"name":"Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75904979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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