首页 > 最新文献

Quality Assurance and Safety of Crops & Foods最新文献

英文 中文
Impact on cardioprotective effect of Psidium guajava leaves extract in streptozotocin-induced Wistar mice with molecular in silico analysis 瓜石榴叶提取物对链脲佐菌素诱导Wistar小鼠心脏保护作用的分子硅分析
Pub Date : 2023-04-27 DOI: 10.15586/qas.v15i2.1261
R. Manikandan, B. Balamuralikrishnan, Arthi Boro, P. Karthika, M. Arun, S. Velayuthaprabhu, Arunkumar Malaisamy, Rengasamy Lakshminarayanan Rengarajan, A. Vijaya Anand
Cardiovascular disease (CVD) and its complications have been regarded as the leading cause of morbidity and mortality. The drugs available in the market are effective to treat CVD, but with many adverse reactions. Nowadays, herbal products are the attention of researchers because of their less adverse effects. In this study, the cardioprotective effects of ethanolic leaves extract of Psidium guajava Linn. (Guava) (P. guajava) were evaluated in streptozotocin (STZ)-treated animal models. Mice acquired for the study were divided into five groups, each consisting of six mice. The toxin-induced mice were treated with the ethanolic leaves extract of P. guajava (300 mg/kg body weight [b.w.]). The results were compared to the standard drug (glibenclamide)-treated mice (3 mg/kg b.w.). The following parameters were considered for further investigations: creatine kinase-muscle brain (CK-MB), creatine kinase (CK), troponin, lysosomal, and mitochondrial enzymes. Then the docking study was accomplished. The levels of cardiac marker enzymes and lysosomal enzymes increased significantly in the toxin-induced mice, while the level of mitochondrial enzyme decreased significantly. During treatment with the ethanolic leaves extract of P. guajava, the levels of all parameters were notably reversed to normal range (P < 0.05). Further, in docking analysis, the interaction of compounds, such as alpha-terpineol, cyclopentanecarboxamide, guaiol (a sesquiterpenoid alcohol), 1H-cyclopropanaphthalene, tetracyclotridecan-9-ol, dormin/abscisic acid, and epiglobulol, with the respective protein molecules, evidenced the cardioprotective effect of P. guajava leaves. Hence, it was concluded that the ethanolic leaves extract of P. guajava leaves have a cardioprotective effect.
心血管疾病及其并发症已被认为是发病率和死亡率的主要原因。目前市场上的药物治疗心血管疾病是有效的,但有许多不良反应。目前,草药产品因其副作用小而受到研究人员的关注。本研究探讨番石榴叶乙醇提取物对心脏的保护作用。(P. guajava)在链脲佐菌素(STZ)处理的动物模型中进行了评价。研究中获得的小鼠被分为五组,每组由六只小鼠组成。用番石榴叶乙醇提取物(300 mg/kg体重[b.w.])治疗中毒小鼠。结果与标准药物(格列苯脲)处理小鼠(3 mg/kg b.w)进行比较。进一步研究考虑以下参数:肌酸激酶-肌脑(CK- mb),肌酸激酶(CK),肌钙蛋白,溶酶体和线粒体酶。对接研究完成。毒素诱导小鼠心肌标志物酶和溶酶体酶水平显著升高,线粒体酶水平显著降低。瓜石榴叶乙醇提取物处理后,各指标水平均显著逆转至正常范围(P < 0.05)。此外,在对接分析中,α -松油醇、环戊anecarboxamide、愈木酚(倍半萜类醇)、1h -环丙炔、四环三烷-9-醇、休眠/脱落酸和表绿酚等化合物与相应蛋白质分子的相互作用证明了瓜石榴叶具有保护心脏的作用。由此可见,番石榴叶乙醇提取物具有一定的心脏保护作用。
{"title":"Impact on cardioprotective effect of Psidium guajava leaves extract in streptozotocin-induced Wistar mice with molecular in silico analysis","authors":"R. Manikandan, B. Balamuralikrishnan, Arthi Boro, P. Karthika, M. Arun, S. Velayuthaprabhu, Arunkumar Malaisamy, Rengasamy Lakshminarayanan Rengarajan, A. Vijaya Anand","doi":"10.15586/qas.v15i2.1261","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1261","url":null,"abstract":"Cardiovascular disease (CVD) and its complications have been regarded as the leading cause of morbidity and mortality. The drugs available in the market are effective to treat CVD, but with many adverse reactions. Nowadays, herbal products are the attention of researchers because of their less adverse effects. In this study, the cardioprotective effects of ethanolic leaves extract of Psidium guajava Linn. (Guava) (P. guajava) were evaluated in streptozotocin (STZ)-treated animal models. Mice acquired for the study were divided into five groups, each consisting of six mice. The toxin-induced mice were treated with the ethanolic leaves extract of P. guajava (300 mg/kg body weight [b.w.]). The results were compared to the standard drug (glibenclamide)-treated mice (3 mg/kg b.w.). The following parameters were considered for further investigations: creatine kinase-muscle brain (CK-MB), creatine kinase (CK), troponin, lysosomal, and mitochondrial enzymes. Then the docking study was accomplished. The levels of cardiac marker enzymes and lysosomal enzymes increased significantly in the toxin-induced mice, while the level of mitochondrial enzyme decreased significantly. During treatment with the ethanolic leaves extract of P. guajava, the levels of all parameters were notably reversed to normal range (P < 0.05). Further, in docking analysis, the interaction of compounds, such as alpha-terpineol, cyclopentanecarboxamide, guaiol (a sesquiterpenoid alcohol), 1H-cyclopropanaphthalene, tetracyclotridecan-9-ol, dormin/abscisic acid, and epiglobulol, with the respective protein molecules, evidenced the cardioprotective effect of P. guajava leaves. Hence, it was concluded that the ethanolic leaves extract of P. guajava leaves have a cardioprotective effect.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73176901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Eriocalyxin B inhibits inflammation induced by CCI-induced microglia activation to relieve neuropathic pain through inhibition of JAK2/STAT3 and NF-κB pathways Eriocalyxin B通过抑制JAK2/STAT3和NF-κB通路,抑制cci诱导的小胶质细胞激活引起的炎症,减轻神经性疼痛
Pub Date : 2023-04-20 DOI: 10.15586/qas.v15i2.1270
Zhifeng Sheng, Xiao-qing Pan
Neuropathic pain is a very troublesome disease that seriously affects human life. Eriocalyxin B (EriB) has been revealed to attenuate various diseases through its anti-inflammatory effects, but its regulatory effects on neuro-pathic pain remains unclear. The paw withdrawal threshold and paw withdrawal thermal latency were detected through mechanical allodynia and thermal hyperalgesia tests. The spinal injury was assessed through hematoxylin and eosin staining. The cell apoptosis was measured through terminal deoxynucleotide transferase-mediated dUTP nick end-labeling assay. The protein expressions were examined through Western blot analysis. The mRNA expression was examined through reverse transcription-quantitative polymerase chain reaction. The ionized calcium-binding adaptor molecule 1 level in the spinal cord was evaluated through immunofluorescence assay. The levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 were measured through enzyme-linked-immunosorbent serologic assay. The chronic constriction injury (CCI) rat model was constructed for the study. Our results demonstrated that EriB relieved CCI-stimulated neuropathic pain and nerve damage. In addition, the enhanced neural apoptosis mediated by CCI induction was reduced after EriB treatment. In addition, EriB inhib-ited CCI-induced microglia activity and inflammation. At last, the Janus kinase 2–signal transducer and activator of transcription 3 (JAK2/STAT3) and nuclear factor kappa B (NF-κB) pathways were activated in CCI rat model, which were attenuated following EriB treatment. Importantly, EriB (10 mg/kg) had a strong effect that was similar to the positive control (1-μg/kg dexmedetomidine), suggesting that EriB may be an effective drug for neuropathic pain. This study demonstrated that EriB inhibited inflammation caused by CCI-induced microglia activation to relieve neuropathic pain through inhibition of JAK2/STAT3 and NF-κB pathways. This study may highlight the regulatory functions of EriB in the treatment of neuropathic pain.
神经性疼痛是一种非常麻烦的疾病,严重影响人类的生活。Eriocalyxin B (EriB)已被发现通过其抗炎作用减轻多种疾病,但其对神经性疼痛的调节作用尚不清楚。通过机械异常性痛和热痛觉过敏试验检测足部戒断阈值和足部戒断热潜伏期。苏木精和伊红染色评估脊髓损伤。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测细胞凋亡。Western blot检测蛋白表达。逆转录-定量聚合酶链反应检测mRNA表达。采用免疫荧光法测定脊髓内钙结合受体分子1的水平。采用酶联免疫吸附血清学法检测肿瘤坏死因子-α、白细胞介素(IL)-1β、IL-6水平。建立慢性缩窄性损伤大鼠模型。我们的研究结果表明,EriB减轻了cci刺激的神经性疼痛和神经损伤。此外,经EriB处理后,CCI诱导的神经细胞凋亡增强有所减弱。此外,EriB抑制cci诱导的小胶质细胞活性和炎症。最后,在CCI大鼠模型中,Janus kinase 2-signal transducer and activator of transcription 3 (JAK2/STAT3)和nuclear factor kappa B (NF-κB)通路被激活,EriB处理后这些通路被减弱。重要的是,EriB (10 mg/kg)具有与阳性对照(1-μg/kg右美托咪定)相似的强效,表明EriB可能是一种治疗神经性疼痛的有效药物。本研究表明,EriB通过抑制JAK2/STAT3和NF-κB通路,抑制cci诱导的小胶质细胞激活引起的炎症,从而缓解神经性疼痛。本研究可能进一步揭示EriB在神经性疼痛治疗中的调控作用。
{"title":"Eriocalyxin B inhibits inflammation induced by CCI-induced microglia activation to relieve neuropathic pain through inhibition of JAK2/STAT3 and NF-κB pathways","authors":"Zhifeng Sheng, Xiao-qing Pan","doi":"10.15586/qas.v15i2.1270","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1270","url":null,"abstract":"Neuropathic pain is a very troublesome disease that seriously affects human life. Eriocalyxin B (EriB) has been revealed to attenuate various diseases through its anti-inflammatory effects, but its regulatory effects on neuro-pathic pain remains unclear. The paw withdrawal threshold and paw withdrawal thermal latency were detected through mechanical allodynia and thermal hyperalgesia tests. The spinal injury was assessed through hematoxylin and eosin staining. The cell apoptosis was measured through terminal deoxynucleotide transferase-mediated dUTP nick end-labeling assay. The protein expressions were examined through Western blot analysis. The mRNA expression was examined through reverse transcription-quantitative polymerase chain reaction. The ionized calcium-binding adaptor molecule 1 level in the spinal cord was evaluated through immunofluorescence assay. The levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 were measured through enzyme-linked-immunosorbent serologic assay. The chronic constriction injury (CCI) rat model was constructed for the study. Our results demonstrated that EriB relieved CCI-stimulated neuropathic pain and nerve damage. In addition, the enhanced neural apoptosis mediated by CCI induction was reduced after EriB treatment. In addition, EriB inhib-ited CCI-induced microglia activity and inflammation. At last, the Janus kinase 2–signal transducer and activator of transcription 3 (JAK2/STAT3) and nuclear factor kappa B (NF-κB) pathways were activated in CCI rat model, which were attenuated following EriB treatment. Importantly, EriB (10 mg/kg) had a strong effect that was similar to the positive control (1-μg/kg dexmedetomidine), suggesting that EriB may be an effective drug for neuropathic pain. This study demonstrated that EriB inhibited inflammation caused by CCI-induced microglia activation to relieve neuropathic pain through inhibition of JAK2/STAT3 and NF-κB pathways. This study may highlight the regulatory functions of EriB in the treatment of neuropathic pain.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86850487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physicochemical evaluation of Prinsepia utilis seed oil (PUSO) and its utilization as a base in pharmaceutical soap formulation 红豆籽油的理化性质评价及其在药用皂制剂中的应用
Pub Date : 2023-04-19 DOI: 10.15586/qas.v15i2.1176
J. Pandey, S. Acharya, Rakshya Bagale, Akriti Gupta, P. Chaudhary, Bikash Rokaya, Manju K.C., P. Aryal, Hari Prasad Devkota
This study aims to evaluate the standard physicochemical parameters, such as iodine value, acid value, saponification value, ester value, refractive index, peroxide value, and viscosity, of Prinsepia utilis (P. utilis) seed oil (PUSO) obtained as hexane extract from seeds, and to formulate ketoconazole soap (2% w/w) by using extracted oil as a base. The quality control standards of the final ketoconazole soap complied with the standards specified in Indian Pharmacopeia. Pharmaceutical soap was obtained by treating PUSO with potassium hydroxide (KOH), undergoing basic saponification. All physicochemical parameters, such as acid value (21.78 mg KOH/g), saponi-fication value (194.13 mg KOH/g), iodine value (99.7 g I2/100 g), ester value (172.35 mg KOH/g), refractive index (1.464), and viscosity (192 centipoises [cps]), conformed to industrial standards, except the peroxide value (19.23 milliequivalent KOH/g). Besides, evaluation of quality control parameters of pharmaceutical soap suggested that its various parameters, such as pH (7.3), foam-forming ability (14.5 cm), foam retention time (15 min), total fatty matter (69.31%), moisture content (10.35%), and drug content (99.37%), were within the acceptable limit. Overall, our study showed that P. utilis base was physicochemically stable and suitable for manufacturing cosmetic products, soaps, and shampoo in an economical manner, rather than using expensive chemical additives, in the pharmaceutical and cosmeceutical industry. Further, this study suggested that therapeutically and commercially successful ketoconazole soap, with all the required quality control parameters, could be manufactured by using naturally available oil at a low cost.
本研究的目的是评价从植物种子中提取的正己烷提取物红草籽油(PUSO)的碘值、酸值、皂化值、酯值、折射率、过氧化值、粘度等标准理化参数,并以提取油为基料制备酮康唑皂(2% w/w)。最终生产的酮康唑皂质量控制标准符合印度药典标准。用氢氧化钾(KOH)对PUSO进行碱性皂化处理,制得药用皂。酸值(21.78 mg KOH/g)、皂化值(194.13 mg KOH/g)、碘值(99.7 g I2/100 g)、酯值(172.35 mg KOH/g)、折射率(1.464)、粘度(192 centipoises [cps])等理化参数除过氧化值(19.23毫当量KOH/g)外,均符合工业标准。对药用皂的质量控制参数进行评价,其pH值(7.3)、泡沫形成能力(14.5 cm)、泡沫保持时间(15 min)、总脂肪含量(69.31%)、水分含量(10.35%)、药物含量(99.37%)等各项参数均在可接受范围内。总的来说,我们的研究表明,在制药和药妆工业中,竹属植物碱具有物理化学稳定性,适合以经济的方式生产化妆品、肥皂和洗发水,而不是使用昂贵的化学添加剂。此外,本研究表明,在治疗和商业上成功的酮康唑皂,具有所有所需的质量控制参数,可以用自然可用的油以低成本制造。
{"title":"Physicochemical evaluation of Prinsepia utilis seed oil (PUSO) and its utilization as a base in pharmaceutical soap formulation","authors":"J. Pandey, S. Acharya, Rakshya Bagale, Akriti Gupta, P. Chaudhary, Bikash Rokaya, Manju K.C., P. Aryal, Hari Prasad Devkota","doi":"10.15586/qas.v15i2.1176","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1176","url":null,"abstract":"This study aims to evaluate the standard physicochemical parameters, such as iodine value, acid value, saponification value, ester value, refractive index, peroxide value, and viscosity, of Prinsepia utilis (P. utilis) seed oil (PUSO) obtained as hexane extract from seeds, and to formulate ketoconazole soap (2% w/w) by using extracted oil as a base. The quality control standards of the final ketoconazole soap complied with the standards specified in Indian Pharmacopeia. Pharmaceutical soap was obtained by treating PUSO with potassium hydroxide (KOH), undergoing basic saponification. All physicochemical parameters, such as acid value (21.78 mg KOH/g), saponi-fication value (194.13 mg KOH/g), iodine value (99.7 g I2/100 g), ester value (172.35 mg KOH/g), refractive index (1.464), and viscosity (192 centipoises [cps]), conformed to industrial standards, except the peroxide value (19.23 milliequivalent KOH/g). Besides, evaluation of quality control parameters of pharmaceutical soap suggested that its various parameters, such as pH (7.3), foam-forming ability (14.5 cm), foam retention time (15 min), total fatty matter (69.31%), moisture content (10.35%), and drug content (99.37%), were within the acceptable limit. Overall, our study showed that P. utilis base was physicochemically stable and suitable for manufacturing cosmetic products, soaps, and shampoo in an economical manner, rather than using expensive chemical additives, in the pharmaceutical and cosmeceutical industry. Further, this study suggested that therapeutically and commercially successful ketoconazole soap, with all the required quality control parameters, could be manufactured by using naturally available oil at a low cost.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86606898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Single-to-combined effects of gelatin and aloe vera incorporated with Shirazi thyme essential oil nanoemulsion on shelf-life quality of button mushroom 明胶、芦荟与白子百里香精油纳米乳对冬菇保质期的影响
Pub Date : 2023-04-18 DOI: 10.15586/qas.v15i2.1241
M. Moosavi‐Nasab, Bahram Behroozi, Hadi Hashemi Gahruie, Samad Tavakoli
Button mushrooms are considered one of the most sensitive agricultural products due to the mechanical damages that can significantly decrease their quality and color. Our study aimed to evaluate the effect of aloe vera and gelatin edible coatings containing Shirazi thyme essential oil nanoemulsion on the shelf life, enzymatic browning, physicochemical, microbiological, textural, and sensorial properties of a button mushroom. Seven samples, including the control (C) and those coated with 1% gelatin (G1), 3% gelatin (G3), 1% aloe vera (A1), 3% aloe vera (A3), 1% gelatin + 2% aloe vera (G1A2), and 2% gelatin + 1% aloe vera (G2A1), were produced. Parameters such as weight loss, pH, firmness, color, microbiological, and sensory attributes were analyzed to evaluate these features. Our results showed that aloe vera and edible gelatin coatings are capable of efficiently maintaining the physico-chemical, microbiological, and sensory properties of button mushrooms. The lower microbial counts, including mesophilic bacteria, yeasts, and molds in treated samples during storage, could be due to the coating containing Shirazi thyme essential oil nanoemulsion. Regarding the efficient role of aloe vera coating (3%) in preventing the growth of microorganisms and maintaining the sensory properties of samples, this treatment could be a promis-ing technique for the preservation of button mushrooms and other vegetables and fruits.
钮扣菇被认为是最敏感的农产品之一,因为机械损伤会显著降低其质量和颜色。本研究旨在评价含白子百里香精油纳米乳的芦荟和明胶可食性涂层对香菇的保质期、酶促褐变、理化、微生物学、质地和感官特性的影响。共制备7个样品,分别为对照(C)、1%明胶(G1)、3%明胶(G3)、1%芦荟(A1)、3%芦荟(A3)、1%明胶+ 2%芦荟(G1A2)、2%明胶+ 1%芦荟(G2A1)。分析了失重、pH值、硬度、颜色、微生物和感官属性等参数来评估这些特征。结果表明,芦荟和可食用明胶涂层能够有效地保持蘑菇的理化、微生物学和感官特性。在储存过程中,处理过的样品中微生物数量较低,包括嗜中温细菌、酵母和霉菌,可能是由于含有白拉子百里香精油纳米乳液的涂层。考虑到芦荟包被(3%)在防止微生物生长和保持样品感官特性方面的有效作用,这种处理可能是一种有前途的技术,用于保存蘑菇和其他蔬菜和水果。
{"title":"Single-to-combined effects of gelatin and aloe vera incorporated with Shirazi thyme essential oil nanoemulsion on shelf-life quality of button mushroom","authors":"M. Moosavi‐Nasab, Bahram Behroozi, Hadi Hashemi Gahruie, Samad Tavakoli","doi":"10.15586/qas.v15i2.1241","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1241","url":null,"abstract":"Button mushrooms are considered one of the most sensitive agricultural products due to the mechanical damages that can significantly decrease their quality and color. Our study aimed to evaluate the effect of aloe vera and gelatin edible coatings containing Shirazi thyme essential oil nanoemulsion on the shelf life, enzymatic browning, physicochemical, microbiological, textural, and sensorial properties of a button mushroom. Seven samples, including the control (C) and those coated with 1% gelatin (G1), 3% gelatin (G3), 1% aloe vera (A1), 3% aloe vera (A3), 1% gelatin + 2% aloe vera (G1A2), and 2% gelatin + 1% aloe vera (G2A1), were produced. Parameters such as weight loss, pH, firmness, color, microbiological, and sensory attributes were analyzed to evaluate these features. Our results showed that aloe vera and edible gelatin coatings are capable of efficiently maintaining the physico-chemical, microbiological, and sensory properties of button mushrooms. The lower microbial counts, including mesophilic bacteria, yeasts, and molds in treated samples during storage, could be due to the coating containing Shirazi thyme essential oil nanoemulsion. Regarding the efficient role of aloe vera coating (3%) in preventing the growth of microorganisms and maintaining the sensory properties of samples, this treatment could be a promis-ing technique for the preservation of button mushrooms and other vegetables and fruits.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80865643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Effects of wine processed Polygonatum polysaccharides on immunomodulatory effects and intestinal microecology in mice 酒制黄精多糖对小鼠免疫调节作用及肠道微生态的影响
Pub Date : 2023-04-11 DOI: 10.15586/qas.v15i2.1227
Tu-lin Lu, Zhi-jun Guo, Xiaoman Li, Peng Chen, Y. Li, Jiu-ba Zhang, Chunqin Mao, Di Ji, Lian-lin Su, Bo Gao, Tu-lin Lu
Polygonatum sibiricum is a traditional Chinese medicinal and food homologous substance, usually used after processing with yellow wine. However, its main active ingredient, polysaccharide, has been less studied after wine processing. This study aimed to investigate the effect of Polygonatum polysaccharides after wine processing on the function of immunosuppressed mice and its related mechanisms. The yellow wine processed Polygonatum (YWPP) polysaccharides were extracted by the water extraction alcohol precipitation method. BALB/c mice were used to establish the immunosuppressive animal model with cyclophosphamide (CTX). The immunomodulatory effect of the YWPP polysaccharides Interleukin 2, interferon γ, immunoglobulin A, Immunoglobulin M, T lymphocyte subsets, and other indexes were detected by enzyme-linked immunosorbent assay, flow cytometry, and other technologies. To study the mechanism of immunomodulation of polysaccharides, 16s rDNA sequencing and Gas Chromatography/Mass Spectrometry (GC/MS) were used to analyze the changes of intestinal microbiota composition, diversity, and the content of short-chain fatty acids (SCFAs). The Spearman method was used to analyze the correlation between gut microbiota and various immune indexes and SCFAs. The results showed that the YWPP polysaccharides could significantly restore the body weight of immunosuppressed mice, improve the spleen and thymus, and regulate peripheral blood cell values. The YWPP polysaccharides can also effectively promote Interleukin 2, interferon γ, immunoglobulin A, and immunoglobulin M in immunosuppressed mice and regulate the expression of immune-related indexes. The YWPP polysaccharides promoted the production of SCFAs, significantly regulated the relative abundance of norank_f__norank_o__RF39, Rikenellaceae_RC9_gut_group, norank_f__ UCG-010, and norank_f__norank_o__Clostridia_UCG-014; reversed CTX-induced metabolic abnormalities of mouse gut microbiota; and normalized them. Spearman correlation analysis showed that the relative abundance of gut microbiota was significantly correlated with various immune indices and SCFAs. In conclusion, the YWPP polysaccharides can improve the immune function of CTX-induced immunocompromised mice, promote cytokine upregulation and the relative abundance of immune-related beneficial bacteria in mice, and regulate the gut microbiota, thereby regulating host immunity. The results of this study will provide a theoretical basis for the practical application of the YWPP polysaccharides, health product development, and diversified product development.
黄精是一种传统的中药材和食品同源物质,通常与黄酒加工后使用。然而,其主要活性成分多糖在葡萄酒加工后的研究较少。本研究旨在探讨酒后黄精多糖对免疫抑制小鼠功能的影响及其机制。采用水提醇沉法提取黄酒黄精多糖。采用环磷酰胺(CTX)建立BALB/c小鼠免疫抑制动物模型。采用酶联免疫吸附法、流式细胞术等技术检测YWPP多糖白细胞介素2、干扰素γ、免疫球蛋白A、免疫球蛋白M、T淋巴细胞亚群等指标的免疫调节作用。为了研究多糖的免疫调节机制,采用16s rDNA测序和气相色谱/质谱(GC/MS)分析了肠道菌群组成、多样性和短链脂肪酸(SCFAs)含量的变化。采用Spearman法分析肠道菌群与各免疫指标及scfa的相关性。结果表明,YWPP多糖能显著恢复免疫抑制小鼠的体重,改善脾脏和胸腺功能,调节外周血细胞值。YWPP多糖还能有效促进免疫抑制小鼠的白细胞介素2、干扰素γ、免疫球蛋白A和免疫球蛋白M,调节免疫相关指标的表达。YWPP多糖促进了scfa的产生,显著调节了norank_f_ norank_o_ rf39、Rikenellaceae_RC9_gut_group、norank_f_ UCG-010和norank_f_ norank_o_ clostridia_ucg -014的相对丰度;逆转ctx诱导的小鼠肠道微生物群代谢异常;把它们标准化。Spearman相关分析显示,肠道菌群相对丰度与各免疫指标和scfa呈显著相关。综上所述,YWPP多糖可以改善ctx诱导的免疫功能低下小鼠的免疫功能,促进小鼠细胞因子上调和免疫相关有益菌的相对丰度,调节肠道菌群,从而调节宿主免疫。本研究结果将为YWPP多糖的实际应用、保健品开发及多元化产品开发提供理论依据。
{"title":"Effects of wine processed Polygonatum polysaccharides on immunomodulatory effects and intestinal microecology in mice","authors":"Tu-lin Lu, Zhi-jun Guo, Xiaoman Li, Peng Chen, Y. Li, Jiu-ba Zhang, Chunqin Mao, Di Ji, Lian-lin Su, Bo Gao, Tu-lin Lu","doi":"10.15586/qas.v15i2.1227","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1227","url":null,"abstract":"Polygonatum sibiricum is a traditional Chinese medicinal and food homologous substance, usually used after processing with yellow wine. However, its main active ingredient, polysaccharide, has been less studied after wine processing. This study aimed to investigate the effect of Polygonatum polysaccharides after wine processing on the function of immunosuppressed mice and its related mechanisms. The yellow wine processed Polygonatum (YWPP) polysaccharides were extracted by the water extraction alcohol precipitation method. BALB/c mice were used to establish the immunosuppressive animal model with cyclophosphamide (CTX). The immunomodulatory effect of the YWPP polysaccharides Interleukin 2, interferon γ, immunoglobulin A, Immunoglobulin M, T lymphocyte subsets, and other indexes were detected by enzyme-linked immunosorbent assay, flow cytometry, and other technologies. To study the mechanism of immunomodulation of polysaccharides, 16s rDNA sequencing and Gas Chromatography/Mass Spectrometry (GC/MS) were used to analyze the changes of intestinal microbiota composition, diversity, and the content of short-chain fatty acids (SCFAs). The Spearman method was used to analyze the correlation between gut microbiota and various immune indexes and SCFAs. The results showed that the YWPP polysaccharides could significantly restore the body weight of immunosuppressed mice, improve the spleen and thymus, and regulate peripheral blood cell values. The YWPP polysaccharides can also effectively promote Interleukin 2, interferon γ, immunoglobulin A, and immunoglobulin M in immunosuppressed mice and regulate the expression of immune-related indexes. The YWPP polysaccharides promoted the production of SCFAs, significantly regulated the relative abundance of norank_f__norank_o__RF39, Rikenellaceae_RC9_gut_group, norank_f__ UCG-010, and norank_f__norank_o__Clostridia_UCG-014; reversed CTX-induced metabolic abnormalities of mouse gut microbiota; and normalized them. Spearman correlation analysis showed that the relative abundance of gut microbiota was significantly correlated with various immune indices and SCFAs. In conclusion, the YWPP polysaccharides can improve the immune function of CTX-induced immunocompromised mice, promote cytokine upregulation and the relative abundance of immune-related beneficial bacteria in mice, and regulate the gut microbiota, thereby regulating host immunity. The results of this study will provide a theoretical basis for the practical application of the YWPP polysaccharides, health product development, and diversified product development.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"442 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82883113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Causes, negative effects, and preventive methods of water pollution in Ethiopia 埃塞俄比亚水污染的原因、负面影响和预防方法
Pub Date : 2023-04-01 DOI: 10.15586/qas.v15i2.1271
Alemu Talema
Water is a finite resource necessary for life and sustainable development. Ethiopia has considerable potential for freshwater resources in East Africa. All living beings depend on water for growth and upkeep, and it is essential for supporting environmental balance and biodiversity. Ethiopia does not able to offer clean water to half of its population, and it does not make good use of its water resources. Many Ethiopians who live in urban areas strug-gle to have access to a consistent and adequate quantity of drinking water. Only a small number of city dwellers have access to enough of clean water. Both human activity and natural events may contaminate water. In develop-ing nations, a major concern for public health and water security is the degradation of quality of water brought on by human activities and weak enforcement. The effectiveness of policies and institutional frameworks to address pollution, as well as their effects on the ecological status and biota of rivers, are little understood in spite of growing pollution levels in many tropical African nations. This article focuses on giving an outline of the reasons for Ethiopia’s water pollution and ways to stop it.
水是生命和可持续发展所必需的有限资源。埃塞俄比亚在东非有相当大的淡水资源潜力。所有生物的生长和维持都依赖于水,水对维持环境平衡和生物多样性至关重要。埃塞俄比亚无法为其一半的人口提供干净的水,也没有很好地利用其水资源。许多生活在城市地区的埃塞俄比亚人很难获得持续和充足的饮用水。只有少数城市居民能获得足够的清洁用水。人类活动和自然事件都可能污染水。在发展中国家,对公共卫生和水安全的一个主要关切是人类活动和执法不力造成的水质退化。尽管许多热带非洲国家的污染程度日益严重,但人们很少了解解决污染问题的政策和体制框架的有效性及其对河流生态状况和生物群的影响。这篇文章的重点是给出了埃塞俄比亚的水污染的原因和方法来阻止它的大纲。
{"title":"Causes, negative effects, and preventive methods of water pollution in Ethiopia","authors":"Alemu Talema","doi":"10.15586/qas.v15i2.1271","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1271","url":null,"abstract":"Water is a finite resource necessary for life and sustainable development. Ethiopia has considerable potential for freshwater resources in East Africa. All living beings depend on water for growth and upkeep, and it is essential for supporting environmental balance and biodiversity. Ethiopia does not able to offer clean water to half of its population, and it does not make good use of its water resources. Many Ethiopians who live in urban areas strug-gle to have access to a consistent and adequate quantity of drinking water. Only a small number of city dwellers have access to enough of clean water. Both human activity and natural events may contaminate water. In develop-ing nations, a major concern for public health and water security is the degradation of quality of water brought on by human activities and weak enforcement. The effectiveness of policies and institutional frameworks to address pollution, as well as their effects on the ecological status and biota of rivers, are little understood in spite of growing pollution levels in many tropical African nations. This article focuses on giving an outline of the reasons for Ethiopia’s water pollution and ways to stop it.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80306553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Physicochemical and microbiological analysis of goose meat 鹅肉的理化和微生物分析
Pub Date : 2023-04-01 DOI: 10.15586/qas.v15i2.1200
Temperature plays a crucial role in the storage of meat. Low temperature effectively slows down the growth rate of microorganisms and enzymatic activity that causes meat spoilage. Frozen storage of meat allows consumers to choose meat’s date of consumption during storage without losing its sensory quality. This work is committed to the effects of freezing and refrigeration on quality and safety of food. The study aims to determine whether different frozen storage temperatures and duration affect the quality of raw goose meat and its processing characteristics after thawing. This information could be a valuable contribution to the scientific literature concerning storage of meat. The focus was on goose meat and sausages. The meat was frozen in three independent runs for 3 and 6 months at -20°C and -70°C. The thawed meat was subjected to physicochemical and microbiological analyses and then turned into raw sausages. Frozen meat sausages showed significantly higher thiobarbituric acid (TBA) values after 2 and 4 weeks of the experiment, compared to the control group. Frozen storage also reduced the growth of Pseudomonas spp. and Enterobacteriaceae for all storage temperatures. Findings of the present study could be used to preserve quality and taste of products during meat processing. 
温度在肉类的储存中起着至关重要的作用。低温有效地减缓了微生物的生长速度和导致肉类变质的酶活性。肉类的冷冻储存可以让消费者在储存期间选择肉类的食用日期,而不会失去其感官品质。本工作致力于研究冷冻和冷藏对食品质量和安全的影响。本研究旨在确定不同的冷冻保存温度和时间是否会影响生鹅肉的品质及其解冻后的加工特性。这一信息可能是对有关肉类储存的科学文献的宝贵贡献。重点是鹅肉和香肠。这些肉分别在-20°C和-70°C的温度下分别冷冻3个月和6个月。解冻后的肉经过理化和微生物分析,然后制成生香肠。冻肉香肠在实验2周和4周后的硫代巴比妥酸(TBA)值明显高于对照组。在所有的储存温度下,冷冻储存也减少了假单胞菌和肠杆菌科的生长。本研究结果可用于在肉类加工过程中保持产品的质量和味道。
{"title":"Physicochemical and microbiological analysis of goose meat","authors":"","doi":"10.15586/qas.v15i2.1200","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1200","url":null,"abstract":"Temperature plays a crucial role in the storage of meat. Low temperature effectively slows down the growth rate of microorganisms and enzymatic activity that causes meat spoilage. Frozen storage of meat allows consumers to choose meat’s date of consumption during storage without losing its sensory quality. This work is committed to the effects of freezing and refrigeration on quality and safety of food. The study aims to determine whether different frozen storage temperatures and duration affect the quality of raw goose meat and its processing characteristics after thawing. This information could be a valuable contribution to the scientific literature concerning storage of meat. The focus was on goose meat and sausages. The meat was frozen in three independent runs for 3 and 6 months at -20°C and -70°C. The thawed meat was subjected to physicochemical and microbiological analyses and then turned into raw sausages. Frozen meat sausages showed significantly higher thiobarbituric acid (TBA) values after 2 and 4 weeks of the experiment, compared to the control group. Frozen storage also reduced the growth of Pseudomonas spp. and Enterobacteriaceae for all storage temperatures. Findings of the present study could be used to preserve quality and taste of products during meat processing.\u0000 ","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90214694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Tubeimoside I ameliorates cerebral ischemia/reperfusion injury through activating SIRT3 Tubeimoside I通过激活SIRT3改善脑缺血/再灌注损伤
Pub Date : 2023-04-01 DOI: 10.15586/qas.v15i2.1263
Shaoyue Huang, Zhen Hong, Kuo Li
Cerebral ischemia-reperfusion (CIR) seriously affects human health and life as it is accompanied by inflammation and apoptosis in brain tissues. Tubeimoside I (TBMS-1) can inhibit neuroinflammation and has neuroprotective effects; however, its effects on ischemia-reperfusion (IR) injury of the brain requires clarity. A mouse cerebral artery occlusion/reperfusion model was used to simulate CIR injury. The neurological function and the area of cerebral infarction were assessed by 2,3,5-triphenyltetrazolium chloride staining. Tumor necrosis factor-α, Interleukin (IL)-1β, IL-6, and IL-10 levels were measured by enzyme-linked-immunosorbent serologic assay kits. Protein blot analysis was performed to assess the expression of apoptosis-related factors. In addition, PC12 (pheo-chromocytoma) cells were treated with oxygen–glucose deprivation/reoxygenation (OGD/R) to establish an in vitro model of CIR injury. The cell viability was measured by cell counting kit-8 assay, and apoptosis levels were detected by flow cytometry. In vivo results indicated that Tubeimoside I reduced cerebral infarct size, decreased inflammatory factor content, inhibited the expression of apoptosis-related factors, including Bax and cleaved-caspase-3 (Asp175), and promoted the expression of survival factor, such as B-cell lymphoma protein 2. In vitro, Tubeimoside I was able to increase cell viability and inhibit apoptosis. Mechanistically, Tubeimoside I was able to enhance both in vivo and in vitro expressions of NAD-dependent deacetylase sirtuin-3 (SIRT3). SIRT3 inhibitor abolished the protective effect of Tubeimoside I on OGD/R-treated cells. Tubeimoside I lessened CIR injury by activating SIRT3. Hence, it could be a potential drug candidate for treating IR injury of the brain.
脑缺血再灌注(Cerebral ischemic -reperfusion, CIR)是一种伴有脑组织炎症和细胞凋亡的疾病,严重影响人类健康和生命。Tubeimoside I (TBMS-1)能抑制神经炎症,具有神经保护作用;然而,其对脑缺血再灌注(IR)损伤的影响尚不清楚。采用小鼠脑动脉闭塞/再灌注模型模拟CIR损伤。采用2,3,5-三苯四唑氯染色法观察大鼠神经功能及脑梗死面积。采用酶联免疫吸附血清学检测试剂盒检测肿瘤坏死因子-α、白细胞介素(IL)-1β、IL-6和IL-10水平。蛋白印迹法检测细胞凋亡相关因子的表达。此外,采用氧-葡萄糖剥夺/再氧化(OGD/R)处理PC12(嗜铬细胞瘤)细胞,建立体外CIR损伤模型。采用细胞计数试剂盒-8检测细胞活力,流式细胞术检测细胞凋亡水平。体内实验结果显示,Tubeimoside I可减小脑梗死面积,降低炎症因子含量,抑制凋亡相关因子Bax、裂解caspase-3 (Asp175)的表达,促进b细胞淋巴瘤蛋白2等存活因子的表达。在体外,Tubeimoside I能够提高细胞活力,抑制细胞凋亡。从机制上讲,Tubeimoside I能够增强nad依赖性去乙酰化酶sirtuin-3 (SIRT3)在体内和体外的表达。SIRT3抑制剂可消除Tubeimoside I对OGD/ r处理细胞的保护作用。Tubeimoside I通过激活SIRT3减轻CIR损伤。因此,它可能是治疗脑IR损伤的潜在候选药物。
{"title":"Tubeimoside I ameliorates cerebral ischemia/reperfusion injury through activating SIRT3","authors":"Shaoyue Huang, Zhen Hong, Kuo Li","doi":"10.15586/qas.v15i2.1263","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1263","url":null,"abstract":"Cerebral ischemia-reperfusion (CIR) seriously affects human health and life as it is accompanied by inflammation and apoptosis in brain tissues. Tubeimoside I (TBMS-1) can inhibit neuroinflammation and has neuroprotective effects; however, its effects on ischemia-reperfusion (IR) injury of the brain requires clarity. A mouse cerebral artery occlusion/reperfusion model was used to simulate CIR injury. The neurological function and the area of cerebral infarction were assessed by 2,3,5-triphenyltetrazolium chloride staining. Tumor necrosis factor-α, Interleukin (IL)-1β, IL-6, and IL-10 levels were measured by enzyme-linked-immunosorbent serologic assay kits. Protein blot analysis was performed to assess the expression of apoptosis-related factors. In addition, PC12 (pheo-chromocytoma) cells were treated with oxygen–glucose deprivation/reoxygenation (OGD/R) to establish an in vitro model of CIR injury. The cell viability was measured by cell counting kit-8 assay, and apoptosis levels were detected by flow cytometry. In vivo results indicated that Tubeimoside I reduced cerebral infarct size, decreased inflammatory factor content, inhibited the expression of apoptosis-related factors, including Bax and cleaved-caspase-3 (Asp175), and promoted the expression of survival factor, such as B-cell lymphoma protein 2. In vitro, Tubeimoside I was able to increase cell viability and inhibit apoptosis. Mechanistically, Tubeimoside I was able to enhance both in vivo and in vitro expressions of NAD-dependent deacetylase sirtuin-3 (SIRT3). SIRT3 inhibitor abolished the protective effect of Tubeimoside I on OGD/R-treated cells. Tubeimoside I lessened CIR injury by activating SIRT3. Hence, it could be a potential drug candidate for treating IR injury of the brain.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90408805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural ingredients and probiotics for lowering cholesterol and saturated fat in dairy products 天然成分和益生菌,降低乳制品中的胆固醇和饱和脂肪
Pub Date : 2023-04-01 DOI: 10.15586/qas.v15i2.1269
Auengploy Chailangka, N. Leksawasdi, Warintorn Ruksiriwanich, K. Jantanasakulwong, P. Rachtanapun, S. Sommano, A. Khaneghah, J. Castagnini, F. Barba, Anbarasu Kumar, Y. Phimolsiripol
Dairy products play a crucial role in ensuring healthy lives and promoting the well-being of people. However, they normally contain high levels of saturated fat and cholesterol which are related to the risk of noncommunicable diseases and other health issues. Our review focuses on the effectiveness of added natural ingredients and probi-otics in dairy products for replacing or lowering cholesterol and saturated fat. This narrative review was concep-tualized to describe: (i) natural ingredients for cholesterol and saturated fat substitution, and (ii) probiotics for lowering both cholesterol and saturated fat. Promising techniques for cholesterol and saturated fat replacement by healthy plant oils, carbohydrate, and protein co-products and their effect on product qualities are discussed. In addition, various probiotics inoculated in dairy products exhibiting effect on saturated fat and cholesterol are also addressed.
乳制品在确保健康生活和促进人民福祉方面发挥着至关重要的作用。然而,它们通常含有高水平的饱和脂肪和胆固醇,这与非传染性疾病和其他健康问题的风险有关。本文综述了乳制品中添加天然成分和益生菌替代或降低胆固醇和饱和脂肪的有效性。这篇叙述性综述的概念是描述:(i)胆固醇和饱和脂肪替代的天然成分,以及(ii)降低胆固醇和饱和脂肪的益生菌。讨论了健康植物油、碳水化合物和蛋白质副产物替代胆固醇和饱和脂肪的有前途的技术及其对产品质量的影响。此外,还讨论了乳制品中接种的各种益生菌对饱和脂肪和胆固醇的影响。
{"title":"Natural ingredients and probiotics for lowering cholesterol and saturated fat in dairy products","authors":"Auengploy Chailangka, N. Leksawasdi, Warintorn Ruksiriwanich, K. Jantanasakulwong, P. Rachtanapun, S. Sommano, A. Khaneghah, J. Castagnini, F. Barba, Anbarasu Kumar, Y. Phimolsiripol","doi":"10.15586/qas.v15i2.1269","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1269","url":null,"abstract":"Dairy products play a crucial role in ensuring healthy lives and promoting the well-being of people. However, they normally contain high levels of saturated fat and cholesterol which are related to the risk of noncommunicable diseases and other health issues. Our review focuses on the effectiveness of added natural ingredients and probi-otics in dairy products for replacing or lowering cholesterol and saturated fat. This narrative review was concep-tualized to describe: (i) natural ingredients for cholesterol and saturated fat substitution, and (ii) probiotics for lowering both cholesterol and saturated fat. Promising techniques for cholesterol and saturated fat replacement by healthy plant oils, carbohydrate, and protein co-products and their effect on product qualities are discussed. In addition, various probiotics inoculated in dairy products exhibiting effect on saturated fat and cholesterol are also addressed.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83916111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Quality characteristics, amino acid composition, and bioactive potential of wheat cookies protein-enriched with unconventional legume protein isolates 非常规豆科分离蛋白富集小麦饼干蛋白的品质特性、氨基酸组成及生物活性潜力
Pub Date : 2023-04-01 DOI: 10.15586/qas.v15i2.1160
P. Sahni, Savita Sharma
The present investigation was intended to utilize protein isolates from forage legumes as unconventional protein ingredients for the development of protein-enriched wheat-based cookies. Alfalfa and dhaincha protein isolates (API and DPI) were supplemented at levels of 2.5, 5, 7.5, and 10%, and the effect of supplementation was eva-lauted on the quality attributes, nutritional composition, amino acid profile, and bioactive potential of cookies. The baking loss, water activity, and spreading (except for 10% API and 5% DPI) decreased, whereas the hardness increased with the increase in supplementation level and the effect was more pronounced with the supplementa-tion of DPI. The non-enzymatic browning index showed that it was not affected by the supplementation. DPI-supplemented cookies showed a color change, whereas no change in the color was observed in API-supplemented cookies. Cookies maintained good sensorial characteristics up to the supplementation of 10% API and 5% DPI and higher supplementation of DPI comprised all the sensorial attributes. Supplementation with protein isolates also enhanced the protein and essential amino acid content, total phenols, flavonoids, and antioxidant activity of the cookies.
本研究旨在利用饲料豆科植物分离蛋白作为非常规蛋白质原料,开发富含蛋白质的小麦饼干。分别在饼干中添加2.5%、5%、7.5%和10%的苜蓿和茶茶分离蛋白(API和DPI),并对饼干的品质属性、营养成分、氨基酸分布和生物活性潜力进行评价。随着添加量的增加,烘焙损失、水分活度和铺展度(除10% API和5% DPI外)均有所降低,而硬度则有所提高,且添加DPI效果更明显。非酶促褐变指数不受添加量的影响。添加dpi的饼干有颜色变化,而添加api的饼干没有颜色变化。添加10%原料药和5% DPI时,甜饼仍保持良好的感觉特性,添加更高的DPI时,甜饼的所有感觉特性都得到了满足。添加分离蛋白还可以提高饼干的蛋白质和必需氨基酸含量、总酚含量、类黄酮含量和抗氧化活性。
{"title":"Quality characteristics, amino acid composition, and bioactive potential of wheat cookies protein-enriched with unconventional legume protein isolates","authors":"P. Sahni, Savita Sharma","doi":"10.15586/qas.v15i2.1160","DOIUrl":"https://doi.org/10.15586/qas.v15i2.1160","url":null,"abstract":"The present investigation was intended to utilize protein isolates from forage legumes as unconventional protein ingredients for the development of protein-enriched wheat-based cookies. Alfalfa and dhaincha protein isolates (API and DPI) were supplemented at levels of 2.5, 5, 7.5, and 10%, and the effect of supplementation was eva-lauted on the quality attributes, nutritional composition, amino acid profile, and bioactive potential of cookies. The baking loss, water activity, and spreading (except for 10% API and 5% DPI) decreased, whereas the hardness increased with the increase in supplementation level and the effect was more pronounced with the supplementa-tion of DPI. The non-enzymatic browning index showed that it was not affected by the supplementation. DPI-supplemented cookies showed a color change, whereas no change in the color was observed in API-supplemented cookies. Cookies maintained good sensorial characteristics up to the supplementation of 10% API and 5% DPI and higher supplementation of DPI comprised all the sensorial attributes. Supplementation with protein isolates also enhanced the protein and essential amino acid content, total phenols, flavonoids, and antioxidant activity of the cookies.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops &amp; Foods","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73399584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
期刊
Quality Assurance and Safety of Crops &amp; Foods
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1