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Moutan Cortex extract inhibited the proliferation and migration of endometrial stromal cells by inhibiting OPN-induced, MAPK-mediated MMP9 activation 牡丹皮提取物通过抑制opn诱导的、mapk介导的MMP9激活来抑制子宫内膜基质细胞的增殖和迁移
Pub Date : 2023-01-10 DOI: 10.15586/qas.v15i1.1235
Jin Liu, Songping Luo, Yue Hu, Xiaopeng Peng
Endometriosis is frequent in women of childbearing age with a morbidity rate of approximately 10%. Its clinical features mainly manifest as dysmenorrhea, chronic pelvic pain, and infertility. However, effective treatments are still lacking; hence, it is necessary to identify an effective and safe treatment strategy on endometriosis. Moutan Cortex extract (MCE) was prepared by decoction. Then, cell proliferation and apoptosis in human endometrial stromal cells (HESCs) were detected by cell counting kit-8, EdU cell proliferation assay, and flow cytometry. Wound-healing assay and transwell migration assay were performed to explore cell migration or invasion. The expression of indicators of downstream signaling pathways was determined by Western blot analysis or enzyme-linked-immunosorbent serologic assay. MCE treatment inhibited cell viability and proliferation in HESCs while promoting cell apoptosis. MCE reduced migration and invasion of HESCs. Furthermore, MCE inhibited osteopontin-induced, mitogen-activated, protein kinase (MAPK)-mediated matrix metallopeptidase 9 (MMP9) activation, and upregulation of OPN reversed the effect of MCE on HESCs. In this study, MCE inhibited the proliferation and migration of endometrial stromal cells by inhibiting OPN induced, MAPK-mediated MMP9 activation. MCE might be a novel treatment strategy on endometriosis.
子宫内膜异位症常见于育龄妇女,发病率约为10%。其临床特征主要表现为痛经、慢性盆腔疼痛、不孕症。然而,仍然缺乏有效的治疗方法;因此,有必要确定一个有效和安全的治疗策略子宫内膜异位症。采用煎法制备牡丹皮提取物(MCE)。采用细胞计数试剂盒-8、EdU细胞增殖试验和流式细胞术检测人子宫内膜基质细胞(HESCs)的增殖和凋亡情况。创面愈合试验和跨井迁移试验探讨细胞迁移或侵袭。通过Western blot分析或酶联免疫吸附血清学检测下游信号通路指标的表达。MCE处理抑制HESCs细胞活力和增殖,促进细胞凋亡。MCE减少HESCs的迁移和侵袭。此外,MCE抑制骨桥蛋白诱导的、丝裂原激活的、蛋白激酶(MAPK)介导的基质金属肽酶9 (MMP9)的激活,上调OPN可逆转MCE对HESCs的影响。在本研究中,MCE通过抑制OPN诱导的、mapk介导的MMP9激活来抑制子宫内膜基质细胞的增殖和迁移。MCE可能是子宫内膜异位症的一种新的治疗策略。
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引用次数: 0
Potential health risk assessment of metals in the muscle of seven wild fish species from the Wujiangdu reservoir, China 乌江都水库7种野生鱼类肌肉中金属的潜在健康风险评价
Pub Date : 2023-01-10 DOI: 10.15586/qas.v15i1.1121
Shenwen Cai, Boping Zeng, Chuntao Li
Concentrations of Cu, Zn, Fe, Mn, Pb, Cd, As, and Hg were analyzed in the surface water and muscle of seven fish species (Carassius auratus, Cyprinus carpio, Hemiculter leucisculus, Pelteobagrus fulvidraco, Silurus meriaionalis, Ctenopharyngodon idellus, and Parabramis pekinensis) from the Wujiangdu reservoir, China. All metal concen-trations in water were lower than grade one water quality values. Mean metal concentrations in the fish muscle decreased in the order: Zn (10.765 mg kg−1) > Fe (8.908 mg kg−1) > Mn (0.373 mg kg−1) > Cu (0.369 mg kg−1) > Pb (0.158 mg kg−1) > As (0.102 mg kg−1) > Hg (0.042 mg kg−1) > Cd (0.024 mg kg−1). Metal concentrations were higher in omnivorous and carnivorous fish than those in herbivorous fish. The bioconcentration factor (BCF) of Hg was much higher than that of other metals in all fish species. The values of target hazard quotient (THQ) and hazard index (HI) were lower than 1 for fishermen and the general population, indicating that there was no considerable noncarcinogenic risk. However, target cancer risk (TR) values were greater than 1.0 × 10−6, indicating that the car-cinogenic risk caused by fish consumption could not be ignored. Several kinds of fish species were not completely safe for human consumption according to the standard of the European Commission. The potential health risks in fishermen were much higher than that in the general population.
分析了乌江都水库7种鱼类(鲫鱼、鲤鱼、银鱼、黄颡鱼、沙颡鱼、沙颡鱼、沙颡鱼和北京对虾)地表水和肌肉中Cu、Zn、Fe、Mn、Pb、Cd、As和Hg的含量。水中金属浓度均低于一级水质值。鱼类肌肉中平均金属浓度降低的顺序为:Zn (10.765 mg kg - 1) > Fe (8.908 mg kg - 1) > Mn (0.373 mg kg - 1) > Cu (0.369 mg kg - 1) > Pb (0.158 mg kg - 1) > As (0.102 mg kg - 1) > Hg (0.042 mg kg - 1) > Cd (0.024 mg kg - 1)。杂食性和肉食性鱼类的金属含量高于草食性鱼类。汞的生物富集系数(BCF)在所有鱼类中均远高于其他金属。渔民和一般人群的目标危害商(THQ)和危害指数(HI)均小于1,表明不存在相当大的非致癌风险。然而,靶癌风险(TR)值大于1.0 × 10−6,表明食用鱼类引起的致癌风险不可忽视。根据欧洲委员会的标准,有几种鱼类对人类食用并不完全安全。渔民的潜在健康风险远高于一般人群。
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引用次数: 26
Heat and mass transfer law during microwave vacuum drying of rice 微波真空干燥过程中的传热传质规律
Pub Date : 2023-01-01 DOI: 10.15586/qas.v15i1.1153
T. Sun, Zhen Yang, H. He
In order to obtain the microwave vacuum drying characteristics of rice and the change in law of temperature and humidity, the mechanism of water diffusion and migration in the drying process was analyzed based on the multiphase flow in porous media, the change model of moisture content was established, and the microwave heating process coupled with electromagnetic field and mass heat field was simulated. The accuracy of the multiphase porous medium model was verified by measuring the moisture changes during rice drying under different vacuum degrees. The results show that the temperature distribution of rice during heating is high in the center and low around, and the vacuum degree hardly affects the change in rice temperature. The capillary pressure in rice gradually increases, and the equilibrium vapor pressure gradually decreases during drying. The estimated moisture content reduced from 0.25 to 0.189, 0.177, and 0.169, and the experimental value decreased from 0.25 to 0.186, 0.177, and 0.167 after drying the rice for 60 min at vacuum of 0.02 MPa, 0.04 MPa, and 0.06 MPa, respectively. The experimental value was in agreement with the calculated value. The higher the vacuum degree, the faster the drying speed, and this finding provided a new idea for improving the drying efficiency.
为获得大米微波真空干燥特性及温湿度变化规律,基于多孔介质中多相流动,分析了干燥过程中水分扩散迁移机理,建立了水分含量变化模型,模拟了微波加热过程中电磁场和质量热场的耦合作用。通过测量不同真空度下大米干燥过程中的水分变化,验证了多相多孔介质模型的准确性。结果表明:稻谷在加热过程中的温度分布是中心高,周围低,真空度对稻谷温度变化影响不大;在干燥过程中,大米的毛细压力逐渐增大,平衡蒸汽压逐渐降低。在0.02 MPa、0.04 MPa和0.06 MPa的真空条件下干燥60 min后,大米的估算含水率从0.25降至0.189、0.177和0.169,实验值从0.25降至0.186、0.177和0.167。实验值与计算值吻合较好。真空度越高,干燥速度越快,这一发现为提高干燥效率提供了新的思路。
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引用次数: 2
Wogonoside improves high-fat diet-induced hyperlipidemia and aortic injury in ApoE -/- mice by upregulating SIRT1 expression 枸杞皂苷通过上调SIRT1表达改善ApoE -/-小鼠高脂肪饮食诱导的高脂血症和主动脉损伤
Pub Date : 2023-01-01 DOI: 10.15586/qas.v15i1.1234
Shao-yi Lin, Yunyun Zhu, Ruoyu Chen, Tingting Hu, Kaihang Wang
This study aimed to evaluate the effect of Wogonoside (Wog), a flavonoid monomer, on hyperlipidemia and explore its possible mechanisms. APOE -/- mice were used to establish the animal model of hyperlipidemia by feeding the high-fat diet (HFD). The serum level of triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA), oil red O staining, and real-time PCR assay. The oxidative stress was measured by ELISA assay. Immunoblot assay and ELISA assay were used to detect the mechanism of Wogonoside on hyperlipidemia. We found that Wogonoside ameliorated lipid metabolism disorders in ApoE -/- mice induced by HFD (P<0.01). Wogonoside also ameliorated HFD-induced inflammation in ApoE -/- mice (P<0.01). Wogonoside ameliorated oxidative stress in HFD-induced ApoE -/- mice (P<0.01). Further study showed that Wogonoside improved HFD-induced hyperlipidemia and inflammation by upregulating SIRT1 expression (P<0.01). These results suggested that Wogonoside has the potential to be used as a promising approach for the intervention of hyperlipidemia.
本研究旨在评价黄酮类单体枸杞皂苷(Wog)对高脂血症的作用,并探讨其可能的机制。采用高脂饲料喂养APOE -/-小鼠,建立高脂血症动物模型。采用酶联免疫吸附试验(ELISA)、油红O染色和实时荧光定量PCR检测血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)和炎症因子水平。采用ELISA法检测氧化应激。采用免疫印迹法和ELISA法检测枸杞皂苷对高脂血症的作用机制。我们发现,枸杞子皂苷能改善HFD诱导的ApoE -/-小鼠脂质代谢紊乱(P<0.01)。枸杞子皂苷还能改善ApoE -/-小鼠hfd诱导的炎症(P<0.01)。枸杞皂苷可改善食油诱导的ApoE -/-小鼠氧化应激(P<0.01)。进一步研究表明,五味子皂苷通过上调SIRT1的表达改善hfd诱导的高脂血症和炎症(P<0.01)。这些结果表明,吴贡皂苷有潜力作为一种有前途的方法来干预高脂血症。
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引用次数: 0
Astragaloside IV inhibits the proliferation and migration of lens epithelial cells induced by TGF-β through the Jagged-1/Notch pathway 黄芪甲苷通过Jagged-1/Notch通路抑制TGF-β诱导的晶状体上皮细胞的增殖和迁移
Pub Date : 2023-01-01 DOI: 10.15586/qas.v15i1.1224
Ling Zhou, Lina Zhong, Yahui Lv
Aberrant proliferation and migration of residual lens epithelial cells (LECs) is the main cause of posterior capsule opacification (PCO) after cataract surgery. The purpose of this study was to confirm the protective effects of Astragaloside IV (AST) in transforming growth factor beta (TGF-β)-induced proliferation and migration of LECs. Human LECs, SRA01/04, were used in this study. MTT assay and EdU assay were used to perform cell viability and proliferation, respectively. The cell cycle was determined using flow cytometry. Cell invasion and migration were determined using transwell assay and wound healing assay, respectively. Protein expression was examined using western blotting. Type I and IV collagen (Col I and IV) and fibronectin (FN) in cells were also detected using immunofluorescence (IF). Cell viability, proliferation, migration and invasion were suppressed by AST in a concentration-dependent manner in TGF-β-treated LECs. AST stopped the cell cycle at G2 phase in a concentration-dependent manner. Expression of Cyclin B1 and CDK1 was suppressed by AST in TGF-β-treated LECs. Protein expression of FN, Col I, Col IV, α-SMA (α-smooth muscle actin), Snail and Slug was downregulated by AST in a concentration-dependent manner. AST inhibited the protein expression of Jagged-1, Notch1, Notch2, Notch3, Hes-1 and Hey-1. Taken together, data from this study demonstrated that AST inhibited LEC viability, proliferation, migration and invasion, as well as fibroblastic differentiation and epithelial-mesenchymal transition process through inhibition of Jagged-1/Notch pathway, providing a potential supplemental treatment for PCO.
残留晶状体上皮细胞(LECs)的异常增殖和迁移是白内障术后后囊膜混浊(PCO)的主要原因。本研究旨在证实黄芪甲苷(Astragaloside IV, AST)对转化生长因子β (TGF-β)诱导的LECs增殖和迁移的保护作用。本研究使用的是人LECs, SRA01/04。MTT法和EdU法分别测定细胞活力和增殖。流式细胞术测定细胞周期。采用transwell法和创面愈合法分别测定细胞的侵袭和迁移。western blotting检测蛋白表达。免疫荧光法(IF)检测细胞内I型和IV型胶原蛋白(Col I和IV)和纤维连接蛋白(FN)。TGF-β处理的细胞活力、增殖、迁移和侵袭均受到AST的浓度依赖性抑制。AST以浓度依赖的方式将细胞周期终止于G2期。TGF-β处理的LECs中,AST可抑制Cyclin B1和CDK1的表达。谷丙转氨酶能以浓度依赖性下调FN、Col I、Col IV、α-SMA (α-平滑肌肌动蛋白)、Snail和Slug蛋白表达。AST抑制jagded -1、Notch1、Notch2、Notch3、Hes-1和Hey-1蛋白的表达。综上所述,本研究的数据表明,AST通过抑制Jagged-1/Notch通路,抑制LEC的活力、增殖、迁移和侵袭,以及成纤维细胞分化和上皮-间质转化过程,为PCO提供了潜在的补充治疗方法。
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引用次数: 0
Screening and identification of antioxidant active ingredients from red wine via spectrum-effect relationship analysis 利用光谱效应关系分析筛选和鉴定红酒中抗氧化活性成分
Pub Date : 2023-01-01 DOI: 10.15586/qas.v15i1.1071
Yuye Gao, Xie-an Yu, Bing Wang, Yibao Jin, Guo Yin, Jue Wang, Yi Lu, Tiejie Wang, Kaishun Bi
Red wine was a prominent constituent of diets across the globe and modern pharmacological studies showed that red wine had the main function of antioxidant. However, the reported screening methods for active ingredients were inefficient and insensitive. In this research, 35 batches of red wine were used to establish the chromatography fingerprint, which were further utilized to screen and identify the antioxidant active ingredients through spectrum-effect relationship analysis. Indeed, the antioxidant capacity of red wine was evaluated by total antioxidant capacity assay kit (ABTS method). After evaluating the antioxidant capacity of samples, correlations were analyzed by Pearson correlation analysis, Spearman correlation analysis, and Gray Relational Analysis (GRA). Moreover, spectrum-effect relationships combined with ultrafast liquid chromatography/quadrupole-time-of-flight mass spectrometry (UFLC-Q-TOF/MS) revealed that the succinic acid, gallic acid, protocatechuic acid, catechin, syringic acid, salicylic acid, homogentisic acid, ellagic acid, isorhamnetin-3-O-glucoside, and trans- resveratrol show antioxidative efficacy. Collectively, the present study established the spectrum-effect relationship strategy of red wine and identified active antioxidant ingredients, which would give reasonable index components for the health function of red wine and provide reference for quick screening of bioactive compounds for food.
红葡萄酒是全球饮食的重要组成部分,现代药理学研究表明,红葡萄酒具有抗氧化的主要功能。然而,现有的有效成分筛选方法效率低且不敏感。本研究以35批红酒为研究对象,建立色谱指纹图谱,并通过谱效关系分析对其抗氧化活性成分进行筛选鉴定。用总抗氧化能力测定试剂盒(ABTS法)评价了红酒的抗氧化能力。评价样品抗氧化能力后,采用Pearson相关分析、Spearman相关分析和灰色关联分析(GRA)进行相关性分析。此外,结合超快液相色谱/四极杆飞行时间质谱(UFLC-Q-TOF/MS)的谱效关系表明,琥珀酸、没食子酸、原儿茶酸、儿茶素、丁香酸、水杨酸、均质酸、鞣花酸、异鼠李苷-3- o -葡萄糖苷和反式白藜芦醇具有抗氧化作用。综上所述,本研究建立了红酒的光谱效应关系策略,并鉴定了红酒中的有效抗氧化成分,为红酒保健功能提供合理的指标成分,为食品生物活性化合物的快速筛选提供参考。
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引用次数: 0
Analysis of hydrochemical characteristics and genesis of water-deficient rivers in China: a case study of the Ciyao River Basin in Shanxi Province 中国缺水河流水化学特征及成因分析——以山西省慈姚河流域为例
Pub Date : 2023-01-01 DOI: 10.15586/qas.v15i1.1213
Hui Xiao-Mei, Yuan Jin, Li Chao, Fan Xiao-jun, Z. Yuan
In order to explore the chemical water characteristics of water-scarce rivers in China, the Ciyao River basin in Shanxi Province was taken as a case study. Water samples of the mainstream and its tributaries were collected in the wet, normal, and dry seasons of 2021. The composition and spatial variation of the main ions in the water body were analyzed using the ion chromatography (IC) instrument. In addition, Pearson correlation analyses were used to evaluate relevant correlation between ion concentrations. The results showed that the overall surface water in the study area was weakly alkaline, and the content of total dissolved solids (TDS) varied greatly, ranging from 702 to 5091 mg/L, with an average of 2897 mg/L. The TDS showed a middle stream > downstream > upstream trend, and the hydrochemical type was Cl • SO4 • HCO3 – Ca • Na. The contents of most ions were significantly changed based on differences in sampling sites and seasons. Natural and human factors influence the chemical characteristics of the river. According to the Gibbs diagram and Piper diagram (Figure 3), the ionic composition of the water body in the basin is mainly affected by the joint action of rock weathering and evaporative crystallization. Carbonate rocks constitute the most significant rock weathering, followed by evaporative and silicate rocks. Wastewater from industrial enterprises, agricultural wastewater, and activities of people’s daily living also have some influence on rock weathering. Cation exchange is also important in forming chemical water components in the Ciyao River. The research results can provide technical references and a basis for regional water environment protection, water resources development and utilization, and watershed eco-hydrology research.
为探讨中国缺水河流的水化学特征,以山西省慈姚河流域为例进行了研究。在2021年湿季、正常季和旱季分别采集干流和支流水样。采用离子色谱法(IC)分析了水体中主要离子的组成及空间变异。此外,采用Pearson相关分析评价离子浓度之间的相关关系。结果表明:研究区地表水总体呈弱碱性,总溶解固形物(TDS)含量变化较大,范围为702 ~ 5091 mg/L,平均为2897 mg/L;TDS呈中游>下游>上游趋势,水化学类型为Cl•SO4•HCO3 - Ca•Na。由于采样地点和季节的不同,大多数离子的含量发生了显著变化。自然和人为因素影响河流的化学特征。由Gibbs图和Piper图(图3)可知,盆地水体的离子组成主要受岩石风化和蒸发结晶共同作用的影响。碳酸盐岩是风化作用最显著的岩石,其次是蒸发岩和硅酸盐岩。工业企业废水、农业废水以及人们日常生活活动对岩石风化也有一定的影响。阳离子交换在慈姚河水化学成分的形成中也起着重要作用。研究成果可为区域水环境保护、水资源开发利用和流域生态水文研究提供技术参考和依据。
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引用次数: 18
Impact of watershed habitat quality based on land use: a case study of taking Ciyao River Basin 基于土地利用的流域生境质量影响——以慈姚河流域为例
Pub Date : 2023-01-01 DOI: 10.15586/qas.v15i1.1212
Hui Xiao-Mei, Yuan Jin, L. Chao, Fan Xiao-jun, Zhou Yuan
Land use pattern is one of the most important factors affecting the quality of habitats, which is taken as the research objective in this study. Based on the land use data of Ciyao river basin from 1985 to 2020, transfer matrix analysis, superposition analysis, and zoning statistics were carried out by ArcGIS. The impact of main threat sources (cultivated land, urban land, and rural residential land) on water quality was calculated using the investment model. The habitat changes and the impact of the habitat quality of the basin on the hydrological and water quality were analyzed. The findings demonstrated that the land-type conversion in Ciyao River Basin from 1985 to 2020 is mainly manifested in the conversion from cultivated land to construction land (94.49 km2), and from grassland to cultivated land (21.59 km2) and forest land (27.15 km2). The habitat quality of the Ciyao River Basin decreased gradually from North to South. The medium and high habitat quality areas are mainly concentrated in the headwater area of the river. Due to the change in land type, the habitat quality in the headwater area of the river significantly improved from 2005 to 2020, while the habitat quality in the confluence area of the Wuliang section significantly declined from 1995 to 2015. Due to the improvements of habitat quality in the source area of the river and water conservation capacity, the total amount of water resources and annual runoff depth of Ciyao River significantly increased from 2005 to 2019 without significant changes in precipitation. The water quality of the Ciyao River has improved due to development of environmental pollution control and the improvement of watershed habitat in 2016–2020.
土地利用方式是影响生境质量的重要因素之一,是本研究的研究目标。基于1985—2020年慈姚河流域土地利用数据,利用ArcGIS软件进行转移矩阵分析、叠加分析和分区统计。利用投资模型计算了主要威胁源(耕地、城市用地和农村居民点用地)对水质的影响。分析了流域生境变化及生境质量对水文水质的影响。结果表明:1985—2020年慈姚河流域土地类型转换主要表现为耕地向建设用地转化(94.49 km2),草地向耕地转化(21.59 km2),林地向林地转化(27.15 km2);慈姚河流域生境质量自北向南逐渐下降。中高生境质量区主要集中在河源区。由于土地类型的变化,2005 - 2020年河源区生境质量显著改善,1995 - 2015年武梁段汇流区生境质量显著下降。2005 - 2019年,由于河源区生境质量和保水能力的改善,慈窑河水资源总量和年径流深度显著增加,降水变化不显著。2016-2020年,由于环境污染治理的发展和流域栖息地的改善,慈腰河水质有所改善。
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引用次数: 12
Complete mitochondrial genome of Pleurocordyceps sinensis (Hypocreales, Ascomycota), a species with uncertain family-level taxonomic assignment 腹子囊菌科下creales中华冬虫夏草(Pleurocordyceps sinensis)的全线粒体基因组
Pub Date : 2022-11-23 DOI: 10.15586/qas.v14i4.1134
Yi Li, Jie Lin, Yong‐Hui Wang, Ke Wang, Ren Wang, Xinyue Zhao, Yi‐Jian Yao
The complete mitochondrial (mt) genome of the ex-type strain of Pleurocordyceps sinensis, a fungus originally isolated from Ophiocordyceps sinensis, was sequenced, and assembled as a single circular DNA of 31,841 bp. The mt genome encoded 15 conserved proteins (rps3, cox1, cox2, cox3, cob, atp6, atp8, atp9, nad1, nad2, nad3, nad4, nad4L, nad5, and nad6), 2 rRNA (rnl and rns), and 25 tRNA, as well as 10 additional non-conserved open reading frames (ncORFs). Comparative analyses showed that mt genomes within the order Hypocreales encoded the same number and synteny of conserved protein coding genes despite an obvious size variation among this group of fungi. Phylogenetic analyses using 14 conserved protein sequences revealed that this fungus may not belong to the current designated family Ophiocordycipitaceae but is more closely related to the species of Clavicipitaceae. The mt genome presented herein would give valuable information on reconstructing the evolutionary history of clavicipitaceous fungi and also aid in resolving the family-level taxonomic assignment of Polycephalomyces s. l. species.
对从冬虫夏草(Ophiocordyceps sinensis)中分离出来的一种真菌——冬虫夏草(Pleurocordyceps sinensis)前型菌株的线粒体全基因组进行了测序,并将其组装为一个长31841 bp的环状DNA。mt基因组编码15个保守蛋白(rps3、cox1、cox2、cox3、cob、atp6、atp8、atp9、nad1、nad2、nad3、nad4、nad4L、nad5和nad6)、2个rRNA (rnl和rns)、25个tRNA以及10个非保守开放阅读框(ncorf)。比较分析表明,尽管在这组真菌中存在明显的大小差异,但在下creales目的mt基因组中编码的保守蛋白编码基因的数量和合成是相同的。对14个保守蛋白序列的系统发育分析表明,该真菌可能不属于目前指定的Ophiocordycipitaceae,而与Clavicipitaceae的物种关系更近。本文提出的mt基因组将为重建锁骨真菌的进化史提供有价值的信息,也有助于解决多头菌属的科级分类分配问题。
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引用次数: 0
Optimization of ultrasound processing parameters for preservation of matured coconut water using a central composite design 采用中心复合设计优化成熟椰子汁超声保鲜工艺参数
Pub Date : 2022-11-16 DOI: 10.15586/qas.v14isp1.1145
Anjitha Jacob, I.P. Sudagar, R. Pandiselvam, P. Rajkumar, M. Rajavel
Mature coconut water (MCW) is a natural beverage and a main by-product of various coconut processing industries such as virgin coconut oil, coconut chips, coconut milk, etc. In spite of huge benefits, MCW’s short shelf life limits its market potential. In this context, the present study investigates the effect of ultrasonic processing parameters, such as amplitude (50%, 60% and 70%) and time (5 min, 10 min and 15 min), on microbial population and quality profile (pH, total soluble solids, total sugars, reducing sugars and non-reducing sugars) of MCW. Central composite design was used to create a multiple linear regression model for each response and to optimize ultrasound processing parameters. The optimal treatment parameters to ensure microbial safety and preserve the nutritional quality of MCW were 60% ultrasonic amplitude and treatment time of 10 min. Total sugars, reducing sugars, non-reducing sugars and microbial load of MCW determined at optimized conditions were 4.92%, 2.804%, 2.13% and 4.79 log cfu/mL, respectively. Ultrasonic treatment was found to be effective in inhibiting microbial growth and maintaining non-reducing sugars of MCW.
成熟椰子水(MCW)是一种天然饮料,是各种椰子加工工业的主要副产品,如初榨椰子油、椰子片、椰奶等。尽管好处巨大,但MCW的保质期短限制了它的市场潜力。在此背景下,本研究探讨了超声处理参数,如振幅(50%,60%和70%)和时间(5分钟,10分钟和15分钟)对MCW微生物种群和质量特征(pH,总可溶性固形物,总糖,还原糖和非还原糖)的影响。采用中心组合设计建立各响应的多元线性回归模型,并对超声处理参数进行优化。在保证微生物安全性和保持营养品质的条件下,超声振幅为60%、处理时间为10 min为最佳处理参数。优化条件下MCW的总糖、还原糖、非还原糖和微生物负荷分别为4.92%、2.804%、2.13%和4.79 log cfu/mL。超声处理能有效抑制微生物生长,维持MCW的非还原糖。
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引用次数: 1
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