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Evaluation of Anxiety and Stress Factors before Surgery From the Perspective of Patients Undergoing Spinal Surgery in the Operating Room of Peymaniyeh Hospital in Jahrom City 2021 术前焦虑和应激因素评估——以贾罗姆市佩曼尼亚医院手术室脊柱手术患者为例
Pub Date : 2021-12-01 DOI: 10.32598/qums.15.9.2469.2
Ebrahim Nasiri Formi, Reza Khorammakan, Zohreh Khodadadi Jahromi
Background and Objectives: Surgery is always a great experience for the patient because surgery of any kind is a threat to the integrity of the body and life. This study was to evaluate the factors of anxiety and stress before surgery in patients who are candidates for spine surgery. Methods: The present study is a descriptive study that was performed on 120 patients who were candidates for spine surgery in of Peymaniyeh Hospital in Jahrom City 2021. Data collection tools were theoretical questionnaire and colleagues. Data were analyzed using SPSS v.22 and descriptive statistics such as frequency, percentage and Pearson correlation coefficient. Results: The results showed that the highest level of anxiety was related to the area of preoperative anxiety (85.1)and in this area, the components of fear of surgery and not knowing the time of surgery (93.3%)and in the area of stressors in relation to Operating room staff for preoperative care also included the components of the patient being left alone until the start of surgery and the observation and encounter of strangers such as anesthesiologists and operating room nurses, causing the most anxiety (88.3%)in patients. Conclusion: Based on the findings of this study, by identifying the factors affecting preoperative anxiety and eliminating them, the amount of preoperative anxiety can be minimized. Therefore, it is suggested to use various methods to educate patients to eliminate the factors affecting anxiety.
背景和目的:手术对病人来说总是一种伟大的经历,因为任何类型的手术都是对身体和生命完整性的威胁。本研究旨在评估脊柱手术候选者术前的焦虑和压力因素。方法:本研究是一项描述性研究,对2021年在Jahrom市Peymaniyeh医院进行脊柱手术的120例患者进行研究。数据收集工具为理论问卷和同事问卷。数据分析采用SPSS v.22及频率、百分比、Pearson相关系数等描述性统计。结果:结果显示,患者焦虑程度最高的是术前焦虑(85.1),其中,害怕手术和不知道手术时间的焦虑(93.3%)和术前护理手术室工作人员的压力源还包括患者独自待到手术开始以及麻醉师和手术室护士等陌生人的观察和遇到的压力源。引起患者焦虑最多(88.3%)。结论:根据本研究结果,识别影响术前焦虑的因素并加以消除,可以最大限度地减少术前焦虑。因此,建议采用多种方法对患者进行教育,消除影响焦虑的因素。
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引用次数: 0
Investigating the Correlation between Health Literacy and Mental Health and Quality of Life Indicators of Inactive Elderly to Lahijan City in 2021 2021年拉海市非活动老年人健康素养与心理健康及生活质量指标的相关性研究
Pub Date : 2021-12-01 DOI: 10.32598/qums.15.9.2411
S. Khodaparast, M. Farajpour Pirbasti, V. Bakhshalipour
Background and Objectives: Health literacy can affect the quality of life of the elderly due to its role in promoting appropriate decisions in the field of mental health. this study was conducted to investigate the correlation between health literacy and mental health and quality of life indicators of inactive elderly to Lahijan City in 2021. Methods: In this descriptive-correlational study, 200 elderly people were selected from Lahijan city. Health Literacy Questionnaire (HELIA), Goldberg Mental Health Questionnaire (GQH-28) and Quality of Life Questionnaire (SF-12) were used to collect data. The data were analyzed by SPSS v.23 using Kolmogorov–Smirnov test (test K–S), T-Test, Analysis of variance (ANOVA) statistical tests and pearson correlation coefficient were analyzed. Results: The results showed that the mean age of the elderly in the study was 64.9 years. The mean score of health literacy was 58.2 in terms of borderline health literacy, mental health 25.11 and quality of life 40.7 which were at the desired level and there was a statistically significant relationship between health literacy and mental health and quality of life indicators (P˂0.001). Conclusion: Considering the relationship between health literacy and mental health and quality of life, it seems necessary to pay more attention to promoting health literacy in the elderly and planning to improve the effective factors in quality of life improvement programs.
背景和目的:健康素养可以影响老年人的生活质量,因为它在促进心理健康领域的适当决定方面发挥作用。本研究旨在探讨2021年拉希詹市非活动老年人健康素养与心理健康和生活质量指标的相关性。方法:采用描述性相关研究的方法,选取拉海建市200名老年人为研究对象。采用健康素养问卷(HELIA)、Goldberg心理健康问卷(GQH-28)和生活质量问卷(SF-12)进行数据收集。数据采用SPSS v.23进行分析,采用K-S检验、t检验、方差分析(ANOVA)统计检验和pearson相关系数分析。结果:研究中老年人平均年龄为64.9岁。健康素养的平均得分为58.2分,心理健康得分为25.11分,生活质量得分为40.7分,处于理想水平,健康素养与心理健康和生活质量指标之间的关系具有统计学意义(P小于0.001)。结论:考虑到健康素养与心理健康和生活质量的关系,有必要更加重视促进老年人健康素养的提高,并计划改善生活质量改善方案中的有效因素。
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引用次数: 1
Nurses’ Knowledge and Attitude Towards Pain Management: A Descriptive- analytical Study in Jahrom City 嘉伦市护士疼痛管理知识与态度的描述性分析研究
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.2069.5
N. Kalani, Ghahreman Bemana, Nafiseh Esmaealpour, Saead Razavi, N. Hatami, M. Radmehr
Background and Objectives: Nurses spend more time with patients than other medical personnel and this requires proper decision making by nurses regarding proper and effective pain control in patients. Therefore, this study was conducted to determine the knowledge and attitude of nurses in Jahrom University of Medical Sciences towards pain management. Methods: This descriptive-analytical study was performed on 142 nurses working in hospitals of Jahrom University of Medical Sciences in 2021. Data collection tools in this study are demographic information questionnaire and nurseschr('39') knowledge and attitude questionnaire about pain. Data analysis was performed using SPSS software version 21 and descriptive statistical tests and inferential tests. Results: The Mean score of nurses chr('39')knowledge about pain management was 10.8±3.3 and the mean score of nurseschr('39') attitude towards pain management was 63.1±7.9. The level of knowledge of the majority of nurses was 130 (91.5%) and the level of attitude of the majority of nurses towards pain management was 112 (78.9%). Only 24 (16.9%) nurses had a positive attitude towards pain management. Pearson correlation coefficient showed a positive and significant relationship between knowledge score and nurseschr('39')attitude score (r=0.248, P=0.003). Conclusion: The results of the present study showed that most nurses do not have sufficient knowledge and attitude towards pain management. Therefore, training and holding retraining classes are recommended for all nurses in modern jobs.
背景和目的:护士比其他医务人员花更多的时间与患者在一起,这就要求护士在适当和有效地控制患者疼痛方面做出正确的决策。因此,本研究旨在了解Jahrom医科大学护士对疼痛管理的知识和态度。方法:采用描述性分析方法,对2021年在贾伦医科大学各医院工作的142名护士进行调查。本研究的数据收集工具为人口统计信息问卷和nurseschr('39')疼痛知识态度问卷。采用SPSS软件第21版进行数据分析,采用描述性统计检验和推理检验。结果:护士对疼痛管理知识的平均得分为10.8±3.3分;护士对疼痛管理态度的平均得分为63.1±7.9分。大多数护士对疼痛管理的知识水平为130(91.5%),大多数护士对疼痛管理的态度水平为112(78.9%)。仅有24名护士(16.9%)对疼痛管理持积极态度。Pearson相关系数显示,知识得分与护士态度得分呈正相关(r=0.248, P=0.003)。结论:本研究结果显示,大多数护士对疼痛管理的知识和态度不足。因此,建议对所有从事现代工作的护士进行培训和再培训。
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引用次数: 0
Evaluation of Sleep Disorders in Patients With Multiple Sclerosis: A Cross-sectional Study in Qom Province, Iran 评估多发性硬化症患者的睡眠障碍:伊朗库姆省的一项横断面研究
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.2308.1
Sepideh Paybast, Mohammad Aghaali, T. Sabokbar, Mohammad Hosien Asi, Sara Rahmati Aram, Freshete Shahab, S. A. Hejazi
Background and Objectives: Multiple sclerosis is an autoimmune disease characterized by inflammation and demyelination of the central nervous system. About half of patients with MS complaint of sleep disorder which could be an indicator of the quality of life. The present study was conducted to evaluate the prevalence of sleep disturbances in patients with multiple sclerosis in Qom province. Methods: We included 120 patients from the MS community list and assessed the participants based on the demographic, Pittsburgh sleep quality index (PSQI) and Global Sleep Assessment (GSA) questionnaire. Data were analyzed using SPSS v. 23 software. Results: We showed that the majority of our patients (74.2) were women with a Mean±SD age of 33.95±8.01 years old. 90(75%) of our patients suffered from at least one sleep disturbance in which difficulty initiating or maintaining sleep were the main complaints. Age was estimated to be the main independent factor significantly associated with sleep disturbance. Except the gender, other studied variables had a considerable impact on certain types of sleep-related disorders. Conclusion: As sleep disorders is frequent and mainly undiagnosed in patients with MS, it is necessary to routine evaluate the patients for sleep disorders.
背景和目的:多发性硬化症是一种以中枢神经系统炎症和脱髓鞘为特征的自身免疫性疾病。大约一半的多发性硬化症患者抱怨睡眠障碍,这可能是生活质量的一个指标。本研究旨在评估库姆省多发性硬化症患者睡眠障碍的患病率。方法:从MS社区名单中选取120例患者,采用人口学、匹兹堡睡眠质量指数(PSQI)和全球睡眠评估(GSA)问卷对参与者进行评估。数据分析采用SPSS v. 23软件。结果:74.2例患者为女性,平均±SD年龄为33.95±8.01岁。90例(75%)患者至少有一种睡眠障碍,其中难以开始或维持睡眠是主要主诉。估计年龄是与睡眠障碍显著相关的主要独立因素。除了性别,其他研究变量对某些类型的睡眠相关障碍也有相当大的影响。结论:睡眠障碍在多发性硬化症患者中较为常见且以未确诊为主,有必要对患者的睡眠障碍进行常规评估。
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引用次数: 0
The Effect of Media on Nursing Image in Societies: A Systematic Review 媒体对社会护理形象的影响:系统回顾
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.2219.2
Sara Amaniyan, Monir Nobahar, Nayyereh Raiesdana
Background and Objectives: The image of nursing has a considerable impact on identity and professional self-esteem in nurses. In this regard, the media can be an effective important factor in drawing a positive or negative image of the nursing profession. Thus, the aim of this study was to explore the effect of media on nursing image in societies. Methods In this systematic review, national and international electronic databases including SID, Magiran, IranMedex, Web of Science, Scopus, and PubMed were systematically searched in October 2020 to retrieve articles published up to 2020, using the keywords nursing profession, nursing image, nursing public image and Social/Mass media. According to the aim of study and inclusion criteria, 15 articles among all obtained articles were assessed to appraise their quality. Finally, the content of final selected articles was reported as extraction tables. Results The search yielded 958 articles, but consideration of inclusion criteria led to final selection of 7 articles. The most important effects of media on the image of nursing included the effect of media on the academic and clinical image of the profession, nursing professional roles, as well as gender and media bias towards the nursing profession. Conclusion More attention to media productions should be considered in order to improve the image of nursing. Additionally, with greater presence, nurses can find in media programs to introduce the reality of their profession the greater help this will be for the voices of nurses to be heard.
背景与目的:护理形象对护士的身份认同和职业自尊有相当大的影响。在这方面,媒体可以是一个有效的重要因素,在绘制积极或消极的形象护理专业。因此,本研究的目的是探讨媒体对社会护理形象的影响。方法系统检索2020年10月SID、Magiran、IranMedex、Web of Science、Scopus、PubMed等国内外电子数据库,检索截至2020年发表的论文,检索关键词为护理专业、护理形象、护理公众形象、社会/大众媒体。根据研究目的和纳入标准,对获得的文献中的15篇进行质量评价。最后将最终选取的文章内容以提取表的形式进行报告。结果共检索到958篇文献,考虑纳入标准,最终筛选出7篇。媒体对护理形象最重要的影响包括媒体对护理专业的学术和临床形象、护理专业角色以及性别和媒体对护理专业的偏见的影响。结论应重视媒体制作,提高护理形象。此外,随着护士的出现,他们可以在媒体节目中找到更多的机会来介绍他们的职业现实,这将更有助于护士的声音被听到。
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引用次数: 1
Assessing the Correlation Between the Level of Depressive Disorder and Quality of life in Employees Working in Covid-19 Reference Hospital in 2020 2020年新冠肺炎参考医院员工抑郁水平与生活质量相关性评估
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.2433
A. Yusefi, P. Nikmanesh, S. Daneshi, M. Bayati, E. Rezabeigi Davarani
Background and Objectives: The widespread prevalence of new coronavirus (COVID-19) worldwide can lead to many mental disorders. COVID-19 Care Frontline Staff, are one of the groups most strongly affected by the mental disorders caused by this disease. This study aimed to assess the correlation between the level of depressive disorder and quality of life in employees working in Covid-19 reference hospital in 2020. Methods: This was descriptive-correlation census study performed on 496 employees of Hazrat Ali Asghar (AS) Hospital in Shiraz as the reference hospital for treatment of COVID-19 in southern Iran. Data collection tools were standard Beck Depression Inventory (BDI) and WHOQOL-BREF quality of life questionnaires. Data were analyzed using descriptive and inferential statistical methods, Pearson correlation coefficient and t-test and ANOVA by SPSS v. 23 software at a significance level of α=0.05. Results: The Mean±SD score of depression and quality of life of the studied employees were 35.8±9.2 (out of 63), 60.7±14.8 (out of 120) respectively. 49.6% and 87.5% of the employees had moderate levels of depression and quality of life, respectively. There was a significant and inverse correlation between depression and employees' quality of life (r=-0.612, P<0.001). Also between depression with education level (P=0.04), marital status (P=0.01), and type of occupation (P=0.03), and between quality of life and marital status (P=0.03) were observed a statistically significant relation. Conclusion: Depression and quality of life of the employees were moderate level. Planning is recommended to improve the mental health of employees, use psychotherapy techniques such as cognitive behavioral therapy and mindfulness, develop and implement stress and anxiety management programs, and provide counseling services.
背景与目的:新型冠状病毒(COVID-19)在世界范围内的广泛流行可导致许多精神障碍。COVID-19护理一线工作人员是受该疾病引起的精神障碍影响最严重的群体之一。本研究旨在评估2020年在Covid-19参考医院工作的员工抑郁水平与生活质量之间的相关性。方法:对设拉子Hazrat Ali Asghar (AS)医院的496名员工进行描述性相关普查研究,该医院是伊朗南部治疗COVID-19的参考医院。数据收集工具为标准贝克抑郁量表(BDI)和WHOQOL-BREF生活质量问卷。数据分析采用描述性和推断性统计方法,Pearson相关系数、t检验和方差分析采用SPSS v. 23软件,显著性水平为α=0.05。结果:受访员工抑郁和生活质量的平均±SD评分分别为35.8±9.2分(63分)和60.7±14.8分(120分)。49.6%的员工抑郁程度中等,87.5%的员工生活质量中等。抑郁与员工生活质量呈显著负相关(r=-0.612, P<0.001)。抑郁与受教育程度(P=0.04)、婚姻状况(P=0.01)、职业类型(P=0.03)、生活质量与婚姻状况(P=0.03)之间均有统计学意义。结论:员工的抑郁和生活质量处于中等水平。建议制定计划,改善员工的心理健康,使用心理治疗技术,如认知行为疗法和正念,制定和实施压力和焦虑管理计划,并提供咨询服务。
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引用次数: 1
Explaining People's Experience of Life in the Context of Covid-19 Epidemic in Qom 解读库姆新冠肺炎疫情背景下人们的生活体验
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.152.3
Z. Khalajinia, Zahra Abedini, Hoda Ahmari Tehran, A. Khoramirad
Background and Objectives: Covid-19 disease is the most important issue in the world today that affects all aspects of life in a society. Therefore, the aim of this study was to explain people's experience of living in conditions of Covid-19 disease. Methods: The present study was performed with a qualitative approach using content analysis by the conventional method in Qom city health centers. The data were collected through in depth semi structured interviews with 20 participants through a purposeful sampling method and analyzed simultaneously. Results: From data analysis, 2 main categories including "lifestyle change" with two subcategories, "change in activities and habits", "change in interests" and other main categories of psychological effects with two subcategories "anxiety and fear" And "feeling lonely" is extracted. Conclusion: According to the findings of this study, the Covid-19 epidemic of Covid-19 disease has been associated with effects on lifestyle and mental health, changes in activities, habits, interests, and problems such as anxiety, sleep problems, stress, tension and loneliness. It is suggested that psychologists and specialists plan and implement effective interventions to reduce the psychological effects of this pandemic and increase people's adaptation to existing conditions. Also encourage people to have an active lifestyle.
背景和目的:2019冠状病毒病是当今世界最重要的问题,影响到社会生活的方方面面。因此,本研究的目的是解释人们在Covid-19疾病条件下的生活经历。方法:本研究采用定性方法,采用常规方法对库姆市卫生院进行内容分析。采用有目的抽样法,对20名参与者进行深度半结构化访谈,并对数据进行同步分析。结果:从数据分析中提取出“生活方式改变”2大类含2个子类,“活动与习惯改变”、“兴趣改变”等心理效应2大类含“焦虑与恐惧”和“感觉孤独”2个子类。结论:根据本研究的发现,Covid-19疾病的流行与生活方式和心理健康的影响、活动、习惯、兴趣的改变以及焦虑、睡眠问题、压力、紧张和孤独等问题有关。建议心理学家和专家规划和实施有效的干预措施,以减少这一流行病的心理影响,并提高人们对现有条件的适应能力。也鼓励人们有一个积极的生活方式。
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引用次数: 2
The Effects of Ethanol and Methanol Extracts of Ziziphus Spina-christi, Peganum Harmala, Salvia Officinalis, and Querqus Brantii on the Growth and Biofilm Formation by Staphylococcus Aureus in Vitro 酸枣、苦参、鼠尾草和槲皮乙醇和甲醇提取物对金黄色葡萄球菌体外生长和生物膜形成的影响
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.2224.1
Maryam Monadi, H. Motamedi, Nazanin Sanei
Background and Objectives: Medicinal plants are one of the most important natural resources in each country. The aim of this study was to evaluate the anti-biofilm effect of ethanol and methanol extracts of Ziziphus spina-christi leaves, Peganum harmala, Salvia officinalis, and Querqus brantii on the growth and formation of biofilm by Staphylococcus aureus. Methods: Following evaluation of the susceptibility of the studied Staphylococcus aureus strain to various antibiotics, the growth inhibitory effect of extracts on the bacterium was investigated by disc diffusion method and then the inhibitory effect of the extracts on biofilm formation was evaluated in 96 well micro-titer plates. The means were compared with ANOVA test. Results: The results from antibacterial effect of the extracts showed that the methanol extract of Peganum harmala and Querqus brantii seeds at 350 and 450 mg/ml concentrations had a greater inhibitory effect on the growth of Staphylococcus aureus than other extracts as well as synthetic antibiotics. The results from anti-biofilm formation effect of the extracts showed that methanol extract of Salvia officinalis leaves and Querqus brantii seeds at 350 mg/ml concentration, and ethanol extract of Salvia officinalis leaves at 350 mg/ml concentration were the lowest concentrations that showed the greatest inhibitory effect on biofilm formation by Staphylococcus aureus. Other extracts inhibited biofilm formation by this bacterium in higher concentrations. The lowest anti-biofilm effect on Staphylococcus aureus was observed by ethanolic and methanolic extracts of Ziziphus spina-christi leaf. Conclusion: The studied extracts in this study, especially the methanol extracts of Salvia officinalis leaves and Peganum harmala seeds, and ethanol extract of Salvia officinalis leaves are good solutions for S. aureus infection and biofilms formation control and are suggested that be evaluated in In vivo experiments and clinical applications.
背景与目的:药用植物是每个国家最重要的自然资源之一。摘要本研究旨在探讨酸枣叶、苦参叶、鼠尾草叶和槲皮叶乙醇和甲醇提取物对金黄色葡萄球菌生长和形成生物膜的抑制作用。方法:在评价所研究的金黄色葡萄球菌菌株对各种抗生素的敏感性的基础上,采用圆盘扩散法研究提取物对金黄色葡萄球菌的生长抑制作用,然后在96孔微滴板上评价提取物对生物膜形成的抑制作用。均数比较采用方差分析。结果:对提取物的抑菌作用结果表明,在350和450 mg/ml浓度下,槟榔和槲皮种子甲醇提取物对金黄色葡萄球菌生长的抑制作用大于其他提取物和合成抗生素。从提取物的抗生物膜效果看,丹参叶甲醇提取物和槲皮种子的浓度为350 mg/ml,丹参叶乙醇提取物的浓度为350 mg/ml,对金黄色葡萄球菌的生物膜形成抑制作用最大。其他提取物在较高浓度下抑制这种细菌的生物膜形成。酸枣叶乙醇和甲醇提取物对金黄色葡萄球菌的抗生物膜作用最低。结论:本研究所研究的提取物,尤其是鼠尾草叶和槟榔籽甲醇提取物和鼠尾草叶乙醇提取物对金黄色葡萄球菌的感染和生物膜的形成具有较好的抑制作用,值得在体内实验和临床应用中进行评价。
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引用次数: 1
Investigating the Correlation Between Academic Procrastination With Emotional Intelligence and Self-efficacy in Operating Room Students of Shiraz University of Medical Sciences in 2019 2019年设拉子医科大学手术室学生学业拖延与情商、自我效能感的相关性研究
Pub Date : 2021-10-01 DOI: 10.32598/qums.15.7.2428.1
Farnoush Azizi, Azadeh Amiri, Mehrdad Rohaninasab, Maryam Paran
Background and Objectives: Academic procrastination can have an adverse effect on students' educational, communicational, health, and career prospects. Therefore, recognizing the factors affecting academic procrastination among students is essential. The aim of this study was to determine the correlation between academic procrastination with emotional intelligence and self-efficacy among operating room students of Shiraz University of Medical Sciences. Methods: This descriptive cross-sectional correlational study was performed on 95 operating room students of Shiraz University of Medical Sciences. Data collection tools included demographic information questionnaires, academic procrastination questionnaire, Scherer general self-efficacy scale and schutte self-report emotional intelligence scale. Data were analyzed using one-way analysis of variance, Pearson correlation coefficient and simultaneous multiple regression by SPSS software version 21. Results: The Mean±‌SD scores of academic procrastination, self-efficacy and emotional intelligence were 20.6±‌8.7, 63.1±‌9.3 and 119.2±‌17.6, respectively. There was a significant and inverse correlation between academic procrastination with self-efficacy (P‌=‌ 0.001, r‌=‌ -0.339) and emotional intelligence (P‌=‌0.007, r‌=‌ -0.276). The results of regression analysis showed that the model of predicting academic procrastination based on self-efficacy is significant (P‌=‌0.035). Academic procrastination based on emotional intelligence was not significant (P‌=‌0.474). Conclusion: The results of this study show that academic procrastination is less among students with higher self-efficacy and emotional intelligence.
背景与目的:学业拖延会对学生的教育、交流、健康和职业前景产生不利影响。因此,认识影响学生学习拖延症的因素是必要的。摘要本研究旨在探讨设拉子医科大学手术室学生学业拖延与情绪智力和自我效能的关系。方法:对设拉子医科大学95名手术室学生进行描述性横断面相关研究。数据收集工具包括人口统计信息问卷、学业拖延问卷、Scherer一般自我效能感量表和schutte自我报告情商量表。采用SPSS软件21版对数据进行单因素方差分析、Pearson相关系数分析和同时多元回归分析。结果:学业拖延、自我效能感和情绪智力的平均±·SD评分分别为20.6±··8.7、63.1±··9.3和119.2±···17.6。学业拖延与自我效能(P→= 0.001,r→= -0.339)、情绪智力(P→= - 0.007,r→= -0.276)呈显著负相关。回归分析结果显示,基于自我效能感的学业拖延预测模型显著(P = 0.035)。学业拖延与情绪智力的关系不显著(P = 0.474)。结论:本研究结果显示,高自我效能感和高情商的学生学业拖延现象较少。
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引用次数: 0
The Effect of Long-term Exposure to Aspartame on Histomorphometric, Histochemical and Expression of P53, Bcl-2 and Caspase-3 Genes in the Ovaries of Mice 长期接触阿斯巴甜对小鼠卵巢组织形态计量学、组织化学及P53、Bcl-2和Caspase-3基因表达的影响
Pub Date : 2021-09-01 DOI: 10.32598/qums.15.6.2350.1
Mohsan Hosseini, H. Morovvati, H. Anbara
Background and Objectives: Aspartame is a non-nutritive and artificial sweetener which is widely used in diet and low calorie products and also in a variety of foods, drugs and hygiene products. The present study has been conducted in order to evaluation of the effects of aspartame on ovaries in female mice. Methods: In this experimental study, 36 adult female mice were randomly divided into four groups of nine each. For three groups aspartame was administered orally with the doses of 40, 80 and 160 mg/kg.BW respectively, and one group as the control group received normal saline for 91 days by gavage. Also a control group was considered. 24 hours after the last treatment, ovarian tissue and blood samples were collected and used for biochemical, histomorphological, histomorphometric, histochemical and gene expression studies. The obtained data were analyzed using One-Way Analysis of Variance (ANOVA) and Tukey’s test at the significance level of P<0.05. Results: In ovarian histomorphometric studies, aspartame at a dose of 160 mg/kg significantly reduced growing follicles and significantly increased atresia follicles. Also in biochemical studies caused a significant decrease in TAC and a significant increase in MDA compared to control group. In connection with the studied genes, aspartame at a dose of 160 mg/kg caused a significant decrease in the expression of Bcl-2 gene and also a significant increase in the expression of P53 and Caspase-3 genes compared to the control group. Conclusion: It seems that high dose aspartame can cause adverse effects on histomorphology, histomorphometry and expression of P53, Bcl-2 and Caspase-3 genes in rat ovaries
背景与目的:阿斯巴甜是一种非营养性的人工甜味剂,广泛应用于饮食和低热量产品以及各种食品、药品和卫生用品中。本研究旨在评价阿斯巴甜对雌性小鼠卵巢的影响。方法:将36只成年雌性小鼠随机分为4组,每组9只。三组小鼠口服阿斯巴甜,剂量分别为40mg /kg、80mg /kg和160mg /kg。1组为对照组,灌胃生理盐水91 d。还考虑了一个对照组。末次治疗后24 h,采集卵巢组织和血液样本,进行生化、组织形态学、组织形态学、组织化学和基因表达研究。所得数据采用单因素方差分析(ANOVA)和Tukey检验,显著性水平P<0.05。结果:在卵巢组织形态学研究中,160mg /kg剂量的阿斯巴甜显著减少生长卵泡,显著增加闭锁卵泡。在生化研究中,与对照组相比,TAC显著降低,MDA显著升高。与所研究的基因有关,160mg /kg剂量的阿斯巴甜使Bcl-2基因的表达显著降低,P53和Caspase-3基因的表达显著增加。结论:大剂量阿斯巴甜可能对大鼠卵巢组织形态学、组织形态学及P53、Bcl-2和Caspase-3基因的表达产生不良影响
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Qom Univ Med Sci J
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