Process Biochemistry最新文献_第8页

Bioconversion of snakeskin shed into bioactive hydrolysate possessing antioxidant and cell proliferative potential 蛇皮蜕皮转化为具有抗氧化和细胞增殖潜能的生物活性水解产物

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-27 DOI: 10.1016/j.procbio.2024.11.031

Vijan Lal Vikash , Deena Praveena Kumar , Parthasarathy Baskaran Sujiritha , Ganesan Ponesakki , Suresh Kumar Anandasadagopan , Numbi Ramudu Kamini

The underutilization of keratin-rich snakeskin shed (SS) to produce bioactive hydrolysate has not been explored to prevent oxidative stress, limiting its potential in biomedical and nutritional applications. This study aims to generate SS hydrolysate (SSH) through SS degradation using a keratinase-producing Bacillus altitudinis VK-1120. The contents of protein and amino acid of SSH were 13.93 ± 0.48 and 5.57 ± 0.06 mg/mL, respectively, after 96 h. Morphological and chemical changes before and after bacterial degradation were analysed. The SSH contained 16 amino acids, including eight essential amino acids, and demonstrated significant antioxidant activity, with a maximum efficacy of 4.16 ± 0.03 μg/mL for 2,2-diphenyl-1-picrylhydrazyl and 1319.53 ± 8.32 μg/mL for hydroxyl radical scavenging activity, equivalent to L-ascorbic acid. Furthermore, Fe²⁺- chelation and ferric-reducing antioxidant power were found to be 581.33 ± 3.33 μg/mL of EDTA-Na₂ and 193.4 ± 5.83 μM of ferrous (II) sulphate equivalent_, respectively. Cell viability and migration analysis showcased the proliferation of fibroblast cells with 50, 200, and 500 ng/mL of SSH at 24 h and a reduction in wound area with increasing SSH concentration. Therefore, SS could be utilized as a bioresource for producing both keratinase and antioxidant-rich hydrolysate for identifying biologically active molecules.

富含角蛋白的蛇皮皮（SS）在生产生物活性水解物以防止氧化应激方面的利用不足，限制了其在生物医学和营养方面的应用潜力。本研究旨在利用产角化酶的高山芽孢杆菌VK-1120降解SS，生成SS水解物（SSH）。96 h后，SSH蛋白和氨基酸含量分别为13.93±0.48和5.57±0.06 mg/mL。分析了细菌降解前后的形态和化学变化。SSH含有16种氨基酸，其中8种必需氨基酸，具有显著的抗氧化活性，对2,2-二苯基-1-苦酰肼的清除活性最高为4.16±0.03 μg/mL，对羟基自由基的清除活性最高为1319.53±8.32 μg/mL，相当于l-抗坏血酸。EDTA-Na2的螯合能力和还原铁的抗氧化能力分别为581.33±3.33 μg/mL和193.4±5.83 μM的硫酸亚铁当量。细胞活力和迁移分析显示，在24 h时，50、200和500 ng/mL的SSH可使成纤维细胞增殖，伤口面积随着SSH浓度的增加而减少。因此，SS可以作为一种生物资源，用于生产角化酶和富含抗氧化剂的水解产物，以鉴定生物活性分子。

{"title":"Bioconversion of snakeskin shed into bioactive hydrolysate possessing antioxidant and cell proliferative potential","authors":"Vijan Lal Vikash , Deena Praveena Kumar , Parthasarathy Baskaran Sujiritha , Ganesan Ponesakki , Suresh Kumar Anandasadagopan , Numbi Ramudu Kamini","doi":"10.1016/j.procbio.2024.11.031","DOIUrl":"10.1016/j.procbio.2024.11.031","url":null,"abstract":"<div><div>The underutilization of keratin-rich snakeskin shed (SS) to produce bioactive hydrolysate has not been explored to prevent oxidative stress, limiting its potential in biomedical and nutritional applications. This study aims to generate SS hydrolysate (SSH) through SS degradation using a keratinase-producing <em>Bacillus altitudinis</em> VK-1120. The contents of protein and amino acid of SSH were 13.93 ± 0.48 and 5.57 ± 0.06 mg/mL, respectively, after 96 h. Morphological and chemical changes before and after bacterial degradation were analysed. The SSH contained 16 amino acids, including eight essential amino acids, and demonstrated significant antioxidant activity, with a maximum efficacy of 4.16 ± 0.03 μg/mL for 2,2-diphenyl-1-picrylhydrazyl and 1319.53 ± 8.32 μg/mL for hydroxyl radical scavenging activity, equivalent to L-ascorbic acid. Furthermore, Fe<sup>2+</sup>- chelation and ferric-reducing antioxidant power were found to be 581.33 ± 3.33 μg/mL of EDTA-Na<sub>2</sub> and 193.4 ± 5.83 μM of ferrous (II) sulphate equivalent<sub>,</sub> respectively. Cell viability and migration analysis showcased the proliferation of fibroblast cells with 50, 200, and 500 ng/mL of SSH at 24 h and a reduction in wound area with increasing SSH concentration. Therefore, SS could be utilized as a bioresource for producing both keratinase and antioxidant-rich hydrolysate for identifying biologically active molecules.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 10-21"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon dots derived from dopamine for potent photodynamic bactericidal and antibiofilm application with biocompatibility and low bacterial resistance 由多巴胺衍生的碳点具有生物相容性和低细菌耐药性，可用于有效的光动力杀菌和抗生物膜应用

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-26 DOI: 10.1016/j.procbio.2024.11.029

Peili Li , Chen Cheng , Boxu Tang , Gan Zhao , Junhua Chen , Yunhe Xu , Da Zhang , Meizhe Yu , Xiang Ke , Zirong Li

Given the ongoing proliferation of bacterial resistance, particularly the rise of “superbugs”, it is imperative to create novel, effective, non-toxic, and non-resistant antibacterial materials and technologies for use in medical settings. Carbon dots derived from dopamine hydrochloride and citric acid (DA-CDs) were effectively produced through a two-step method, displaying photodynamic therapy (PDT) characteristics with low-drug resistance. Only a concentration of 0.125 μg mL⁻¹ of DA-CDs can effectively break down established S. aureus biofilms when exposed to blue light (450–500 nm), resulting in a biofilm destruction rate of 96.2 %. And after 28 passages, the sensitivity of S. aureus and E. coli to DA-CDs when irradiated with blue light stays the same. The ultra-high negative zeta potential of DA-CDs (-35 mV) and excess intracellular reactive oxygen species (ROS) induced by DA-CDs accounted for their antibacterial and antibiofilm effects. Furthermore, DA-CDs exhibit outstanding biocompatibility without causing harm to red blood cells (RBCs), 3T3 Cells, bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). The DA-CDs from this study can effectively kill harmful bacteria and destroy established biofilms using PDT, providing a new alternative to antibiotics and a valuable tool for exploring new treatments for biofilm-related infections.

鉴于细菌耐药性的持续扩散，特别是“超级细菌”的兴起，必须创造用于医疗环境的新型、有效、无毒和无耐药性的抗菌材料和技术。以盐酸多巴胺和柠檬酸（DA-CDs）为原料，采用两步法制备碳点，具有光动力治疗（PDT）特性，耐药低。当暴露于蓝光（450-500 nm）时，浓度为0.125 μg mL−1的DA-CDs可以有效地破坏已建立的金黄色葡萄球菌生物膜，生物膜破坏率为96.2 %。经过28次传代，金黄色葡萄球菌和大肠杆菌在蓝光照射下对DA-CDs的敏感性保持不变。DA-CDs的超高zeta负电位（-35 mV）和过量的细胞内活性氧（ROS）是其抗菌和抗生物膜作用的主要原因。此外，DA-CDs具有出色的生物相容性，不会对红细胞（rbc）、3T3细胞、骨髓间充质干细胞（BMSCs）和人脐静脉内皮细胞（HUVECs）造成伤害。本研究的DA-CDs可以有效地杀死有害细菌并利用PDT破坏已建立的生物膜，为抗生素提供了新的替代方案，并为探索生物膜相关感染的新治疗方法提供了有价值的工具。

{"title":"Carbon dots derived from dopamine for potent photodynamic bactericidal and antibiofilm application with biocompatibility and low bacterial resistance","authors":"Peili Li , Chen Cheng , Boxu Tang , Gan Zhao , Junhua Chen , Yunhe Xu , Da Zhang , Meizhe Yu , Xiang Ke , Zirong Li","doi":"10.1016/j.procbio.2024.11.029","DOIUrl":"10.1016/j.procbio.2024.11.029","url":null,"abstract":"<div><div>Given the ongoing proliferation of bacterial resistance, particularly the rise of “superbugs”, it is imperative to create novel, effective, non-toxic, and non-resistant antibacterial materials and technologies for use in medical settings. Carbon dots derived from dopamine hydrochloride and citric acid (DA-CDs) were effectively produced through a two-step method, displaying photodynamic therapy (PDT) characteristics with low-drug resistance. Only a concentration of 0.125 μg mL<sup>−1</sup> of DA-CDs can effectively break down established <em>S. aureus</em> biofilms when exposed to blue light (450–500 nm), resulting in a biofilm destruction rate of 96.2 %. And after 28 passages, the sensitivity of <em>S. aureus</em> and <em>E. coli</em> to DA-CDs when irradiated with blue light stays the same. The ultra-high negative zeta potential of DA-CDs (-35 mV) and excess intracellular reactive oxygen species (ROS) induced by DA-CDs accounted for their antibacterial and antibiofilm effects. Furthermore, DA-CDs exhibit outstanding biocompatibility without causing harm to red blood cells (<em>RBCs</em>), <em>3T3</em> Cells, bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). The DA-CDs from this study can effectively kill harmful bacteria and destroy established biofilms using PDT, providing a new alternative to antibiotics and a valuable tool for exploring new treatments for biofilm-related infections.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 222-232"},"PeriodicalIF":3.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antihypertensive peptides from hydrolyzed proteins of Pleurotus spp.: Production, in vitro digestion and identification 侧耳菇水解蛋白抗高血压肽的制备、体外消化和鉴定

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-23 DOI: 10.1016/j.procbio.2024.11.027

Daniela Fernanda Lima de Carvalho Cavenaghi , Wander Miguel de Barros , Ruann Janser Soares de Castro

This study aimed to investigate in vitro antihypertensive property of protein hydrolysates from Pleurotus spp. mushrooms obtained through enzymatic hydrolysis. Flavourzyme™ 500 L, Alcalase™ 2.4 L and Neutrase™ 0.8 L were used alone and in combination using an experimental design of mixtures. Antihypertensive activity was determined by angiotensin-converting enzyme (ACE) inhibition and the simulated in vitro digestion was performed according to the INFOGEST protocol. Results showed that most of the protein hydrolysates obtained displayed higher ACE inhibitory activity than non-hydrolyzed protein, ranging from 15.76 % and 50.87 % inhibition. The highest ACE inhibition and the lowest TCA soluble protein content (52.09 %) were detected for the protein hydrolysates obtained by using the binary mixture of Flavourzyme™ 500 L and Alcalase™ 2.4 L in equal proportions. Hydrolysis kinetics showed no significant difference in ACE inhibitory activity between 20 and 120 minutes of enzymatic reaction. The fraction with molecular weight between 3 and 5 kDa obtained after ultrafiltration showed the most contribution for ACE inhibitory activity (47 % inhibition). After simulated digestion, the hydrolysates maintained a significant ACE inhibition capacity, indicating the resistance of the peptides to the action of gastrointestinal enzymes. Through proteomic analysis, 26 peptides were identified and the sequences LPILP, IPLLP, PLLPQ and VIQYDPPQ were considered potentially antihypertensive.

本研究旨在研究酶解平菇蛋白水解产物的体外降压特性。Flavourzyme™500 L、Alcalase™2.4 L和Neutrase™0.8 L单独或联合使用，采用混合实验设计。通过抑制血管紧张素转换酶（ACE）测定抗高血压活性，并根据INFOGEST方案进行模拟体外消化。结果表明，大多数蛋白水解产物的ACE抑制活性高于非水解蛋白，抑制率为15.76 %和50.87 %。Flavourzyme™500 L和Alcalase™2.4 L以等比例混合得到的蛋白水解产物，ACE抑制率最高，TCA可溶性蛋白含量最低（52.09 %）。水解动力学表明，酶促反应20和120 min时ACE抑制活性无显著差异。超滤后得到的分子量在3 ~ 5 kDa之间的部分对ACE抑制活性贡献最大（47 %）。模拟消化后，水解产物保持了显著的ACE抑制能力，表明肽对胃肠道酶的作用具有抗性。通过蛋白质组学分析，鉴定出26个多肽序列，认为LPILP、IPLLP、PLLPQ和VIQYDPPQ具有潜在的抗高血压作用。

{"title":"Antihypertensive peptides from hydrolyzed proteins of Pleurotus spp.: Production, in vitro digestion and identification","authors":"Daniela Fernanda Lima de Carvalho Cavenaghi , Wander Miguel de Barros , Ruann Janser Soares de Castro","doi":"10.1016/j.procbio.2024.11.027","DOIUrl":"10.1016/j.procbio.2024.11.027","url":null,"abstract":"<div><div>This study aimed to investigate <em>in vitro</em> antihypertensive property of protein hydrolysates from <em>Pleurotus</em> spp. mushrooms obtained through enzymatic hydrolysis. Flavourzyme™ 500 L, Alcalase™ 2.4 L and Neutrase™ 0.8 L were used alone and in combination using an experimental design of mixtures. Antihypertensive activity was determined by angiotensin-converting enzyme (ACE) inhibition and the simulated <em>in vitro</em> digestion was performed according to the INFOGEST protocol. Results showed that most of the protein hydrolysates obtained displayed higher ACE inhibitory activity than non-hydrolyzed protein, ranging from 15.76 % and 50.87 % inhibition. The highest ACE inhibition and the lowest TCA soluble protein content (52.09 %) were detected for the protein hydrolysates obtained by using the binary mixture of Flavourzyme™ 500 L and Alcalase™ 2.4 L in equal proportions. Hydrolysis kinetics showed no significant difference in ACE inhibitory activity between 20 and 120 minutes of enzymatic reaction. The fraction with molecular weight between 3 and 5 kDa obtained after ultrafiltration showed the most contribution for ACE inhibitory activity (47 % inhibition). After simulated digestion, the hydrolysates maintained a significant ACE inhibition capacity, indicating the resistance of the peptides to the action of gastrointestinal enzymes. Through proteomic analysis, 26 peptides were identified and the sequences LPILP, IPLLP, PLLPQ and VIQYDPPQ were considered potentially antihypertensive.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"149 ","pages":"Pages 1-9"},"PeriodicalIF":3.7,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in biomarkers detection of various diseases by biosensors derived from optical chromogenic polymeric transducers: A review 利用生物传感器检测各种疾病的生物标记物的最新进展：综述

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-21 DOI: 10.1016/j.procbio.2024.11.023

Vikrant Sharma, Disha Kapil, Baljit Singh

The present review article discusses the various sensors for biomarker detection available for multiple diseases with the scope of helping and encouraging researchers to design devices for the effective and early detection of diseases. In general, these sensors are classified into various types based on bio-recognition by detection systems or transducers. However, based on optical transducers, these are further divided into chromogenic, luminogenic, chemiluminescence, and optical fiber. Here in this article, attempts have been made for the analysis of various analytes or biomarkers of various diseases using different polymeric systems under the classification of optical chromogenic transducers. Optical detection of biomarkers in the sample solution is obtained via a biochemical reaction that causes changes in optical properties. Herein, results can be easily analyzed by a color change produced via ligand-analyte interaction, which can be measured to help in the diagnosis of various diseases. There are various types of renal biomarkers, which can be used as an indication of the functioning of the kidney like glucose (GL), uric acid (UA), urea, creatinine (CRN), and albumin (AL). GL is also an important biomarker for diabetes mellitus. For urinary tract infections (UTIs), nitrite is an important biomarker in urine samples. Bilirubin is used as a biomarker for liver damage. Increased chloride concentration in the sweat samples can be used as a biomarker for the disease cystic fibrosis. An elevated cholesterol level inside the body acts as a biomarker for cardiac arrest. Lactate appears to be a powerful predictor of sepsis. This review mainly deals with biochemical reactions for the detection of various biomarkers of diseases. Chemical aspects of the reactions are briefly discussed for their colorimetric evaluation to determine the biomarkers. This article also discusses various polymeric supports available for developing point-of-care testing (POCT) strips.

本综述文章讨论了可用于多种疾病生物标志物检测的各种传感器，旨在帮助和鼓励研究人员设计有效和早期检测疾病的设备。一般来说，这些传感器可根据检测系统或传感器的生物识别能力分为不同类型。然而，根据光学传感器，这些传感器又可进一步分为显色型、发光型、化学发光型和光纤型。在本文中，我们尝试使用不同的聚合物系统分析各种分析物或各种疾病的生物标记物，并将其归类为光学显色传感器。样品溶液中生物标记物的光学检测是通过引起光学特性变化的生化反应实现的。通过配体与分析物相互作用产生的颜色变化，可以轻松地分析结果，测量结果有助于诊断各种疾病。肾脏生物标记物种类繁多，可用于指示肾脏功能，如葡萄糖（GL）、尿酸（UA）、尿素、肌酐（CRN）和白蛋白（AL）。葡萄糖（GL）也是糖尿病的重要生物标志物。对于尿路感染（UTI），亚硝酸盐是尿样中的重要生物标志物。胆红素是肝损伤的生物标志物。汗液样本中氯化物浓度的增加可作为囊性纤维化疾病的生物标志物。体内胆固醇水平升高可作为心脏骤停的生物标志物。乳酸盐似乎是败血症的有力预测指标。这篇综述主要涉及用于检测各种疾病生物标志物的生化反应。文章简要讨论了这些反应的化学方面，以便对其进行比色评估，从而确定生物标记物。本文还讨论了可用于开发护理点检测（POCT）试纸条的各种聚合物支持物。

{"title":"Recent advances in biomarkers detection of various diseases by biosensors derived from optical chromogenic polymeric transducers: A review","authors":"Vikrant Sharma, Disha Kapil, Baljit Singh","doi":"10.1016/j.procbio.2024.11.023","DOIUrl":"10.1016/j.procbio.2024.11.023","url":null,"abstract":"<div><div>The present review article discusses the various sensors for biomarker detection available for multiple diseases with the scope of helping and encouraging researchers to design devices for the effective and early detection of diseases. In general, these sensors are classified into various types based on bio-recognition by detection systems or transducers. However, based on optical transducers, these are further divided into chromogenic, luminogenic, chemiluminescence, and optical fiber. Here in this article, attempts have been made for the analysis of various analytes or biomarkers of various diseases using different polymeric systems under the classification of optical chromogenic transducers. Optical detection of biomarkers in the sample solution is obtained via a biochemical reaction that causes changes in optical properties. Herein, results can be easily analyzed by a color change produced via ligand-analyte interaction, which can be measured to help in the diagnosis of various diseases. There are various types of renal biomarkers, which can be used as an indication of the functioning of the kidney like glucose (GL), uric acid (UA), urea, creatinine (CRN), and albumin (AL). GL is also an important biomarker for diabetes mellitus. For urinary tract infections (UTIs), nitrite is an important biomarker in urine samples. Bilirubin is used as a biomarker for liver damage. Increased chloride concentration in the sweat samples can be used as a biomarker for the disease cystic fibrosis. An elevated cholesterol level inside the body acts as a biomarker for cardiac arrest. Lactate appears to be a powerful predictor of sepsis. This review mainly deals with biochemical reactions for the detection of various biomarkers of diseases. Chemical aspects of the reactions are briefly discussed for their colorimetric evaluation to determine the biomarkers. This article also discusses various polymeric supports available for developing point-of-care testing (POCT) strips.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 191-221"},"PeriodicalIF":3.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of puffed feather to produce functional biosurfactants by Chitinophaga eiseniae 4 H 嗜壳甲壳虫降解膨化羽毛以产生功能性生物表面活性剂 4 H

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.024

Chen Yuan , Jiaxing Wang , Jin Chuan Wu , Qingxin Li

This study demonstrated that feathers, primarily composed of keratin, can be converted into eco-friendly products using the newly isolated strain Chitinophaga eiseniae 4 H (C.eiseniae 4 H), which degrades puffed feather powder by over 90 % in 36 hours. The PFP degradation solution's surface tension dropped to 32.35 ± 0.096 mN/m after 60 hours, with inhibition zones of 12 mm and 14 mm observed for the 48-hour and 72-hour hydrolysates. Additionally, the concentrations of total amino acids and essential amino acids increased significantly after 24 hours of hydrolysis. This approach provides a novel strategy to utilize feather-containing waste for producing value-added biosurfactants with antibacterial and antioxidant properties.

这项研究表明，主要由角蛋白组成的羽毛可以利用新分离的菌株Chitinophaga eiseniae 4 H（C.eiseniae 4 H）转化为环保产品，该菌株可在36小时内降解90%以上的膨化羽毛粉。60 小时后，全氟辛烷磺酸降解溶液的表面张力降至 32.35 ± 0.096 mN/m，48 小时和 72 小时水解物的抑制区分别为 12 mm 和 14 mm。此外，水解 24 小时后，总氨基酸和必需氨基酸的浓度显著增加。这种方法为利用含羽毛废物生产具有抗菌和抗氧化特性的高附加值生物表面活性剂提供了一种新策略。

引用次数: 0

Integration of 2,3-butanediol production and extraction of active components from Chinese herbs by Bacillus licheniformis and evaluation of fermentation products in vitro 地衣芽孢杆菌生产 2,3-丁二醇与提取中草药活性成分的整合及发酵产物的体外评价

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.022

Yanting Xu, Jing Zhuang, Shuo Wang, Jianying Dai, Zhi-Long Xiu

The aim of this work was to develop an integrated process of 2,3-butanediol production and extraction of active components from Chinese herbs via fermentation, and evaluate their potential as cosmetic ingredients in vitro. A GRAS strain DL2–2 of Bacillus licheniformis was isolated and the fermentation medium was optimized using the ingredients that could be used in cosmetics. Four types of Chinese herbs were added solely or in combination into the fermentation medium to perform 2,3-butanediol production and active components extraction simultaneously. The release of polysaccharides and flavonoids was promoted, and the antioxidant, whitening and antibacterial abilities were improved. The highest antioxidant and antibacterial activity was obtained from the ferment filtrate of Dendrobium devonianum and Gastrodia elata f. glauca, resulting from the highest contents of flavonoids (0.44 g/L) and polyphenols (0.31 g/L), and 42.2 g/L 2,3-butanediol was also produced. The highest polysaccharide concentration was obtained from the ferment filtrate containing Polygonatum kingianum, which exhibited the highest inhibitory effect on tyrosinase (65 %), similar to that of 1 g/L arbutin. Therefore, the filtrate of 2,3-butanediol-Chinese herbal fermentation broth is promising as an ingredient in the production of natural high-value cosmetics.

这项工作的目的是开发一种通过发酵生产 2,3-丁二醇和提取中草药活性成分的综合工艺，并在体外评估其作为化妆品成分的潜力。研究人员分离了地衣芽孢杆菌的 GRAS 菌株 DL2-2，并利用可用于化妆品的成分对发酵培养基进行了优化。将四种中草药单独或混合加入发酵培养基中，同时进行 2,3-丁二醇的生产和活性成分的提取。结果表明，2,3-丁二醇能促进多糖和黄酮类化合物的释放，提高抗氧化、美白和抗菌能力。铁皮石斛和天麻的发酵滤液中黄酮类化合物（0.44 克/升）和多酚类化合物（0.31 克/升）含量最高，抗氧化和抗菌活性也最高，同时还产生了 42.2 克/升的 2,3-丁二醇。含有何首乌的发酵滤液中多糖浓度最高，对酪氨酸酶的抑制作用最强（65%），与 1 g/L 熊果苷的抑制作用相似。因此，2,3-丁二醇-中草药发酵液滤液有望成为生产天然高价值化妆品的原料。

{"title":"Integration of 2,3-butanediol production and extraction of active components from Chinese herbs by Bacillus licheniformis and evaluation of fermentation products in vitro","authors":"Yanting Xu, Jing Zhuang, Shuo Wang, Jianying Dai, Zhi-Long Xiu","doi":"10.1016/j.procbio.2024.11.022","DOIUrl":"10.1016/j.procbio.2024.11.022","url":null,"abstract":"<div><div>The aim of this work was to develop an integrated process of 2,3-butanediol production and extraction of active components from Chinese herbs via fermentation, and evaluate their potential as cosmetic ingredients in vitro. A GRAS strain DL2–2 of <em>Bacillus licheniformis</em> was isolated and the fermentation medium was optimized using the ingredients that could be used in cosmetics. Four types of Chinese herbs were added solely or in combination into the fermentation medium to perform 2,3-butanediol production and active components extraction simultaneously. The release of polysaccharides and flavonoids was promoted, and the antioxidant, whitening and antibacterial abilities were improved. The highest antioxidant and antibacterial activity was obtained from the ferment filtrate of <em>Dendrobium devonianum</em> and <em>Gastrodia elata f. glauca</em>, resulting from the highest contents of flavonoids (0.44 g/L) and polyphenols (0.31 g/L), and 42.2 g/L 2,3-butanediol was also produced. The highest polysaccharide concentration was obtained from the ferment filtrate containing <em>Polygonatum kingianum</em>, which exhibited the highest inhibitory effect on tyrosinase (65 %), similar to that of 1 g/L arbutin. Therefore, the filtrate of 2,3-butanediol-Chinese herbal fermentation broth is promising as an ingredient in the production of natural high-value cosmetics.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 150-156"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laccase-mediated synthesis of phloridzin oligomers: An insight into the influence of reaction conditions and evaluation of oligomers’ biological activity 拉克酶介导的氯唑嗪低聚物合成：深入了解反应条件的影响并评估低聚物的生物活性

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.021

Ana Vukoičić , Ana Milivojević , Ljiljana Tolić Stojadinović , Svetlana Grujić , Ljubodrag Vujisić , Vele Tešević , Dejan Bezbradica

The effect of key experimental factors on phloridzin oligomerization catalyzed by Trametes versicolor laccase was investigated, and biological activity of synthesized oligomers was evaluated. Mass spectrometry analysis of the obtained reaction mixture showed the presence of oligomers with the degrees of polymerization two and three with dimer as the major product. The detected masses of the formed oligomers indicated the repetition of phloridzin units in their structure and the loss of two hydrogen atoms during the linkage formation, while NMR analysis of the major dimer confirmed the presence of two phloridzin molecules linked via a C5-C5 bond. The effect of temperature, substrate, and enzyme concentration on phloridzin conversion and products’ yield was examined, and for achieving the highest products yield (4.1 mg/ml), optimal factors were 40 °C temperature, 5 mg/ml phloridzin concentration and 0.5 mg/ml laccase concentration. Synthesized oligomers showed good antioxidant activity compared to phloridzin and very good potential to be used as skin prebiotics since they exhibited inhibitory effect on opportunistic pathogen Staphylococcus aureus and stimulating effect on commensal bacteria Staphylococcus epidermidis in a broad range of tested concentrations. The highest prebiotic capacity was demonstrated at a concentration of 0.0195 mg/ml in S. epidermidis and S. aureus co-culture.

研究了关键实验因素对Trametes versicolor漆酶催化氯啶嗪低聚作用的影响，并对合成的低聚物的生物活性进行了评估。对所得反应混合物的质谱分析表明，存在聚合度为 2 和 3 的低聚物，二聚物是主要产物。所形成的低聚物的质量检测结果表明，它们的结构中存在重复的氯啶单元，并且在连接形成过程中失去了两个氢原子，而主要二聚物的核磁共振分析则证实存在两个通过 C5-C5 键连接的氯啶分子。研究了温度、底物和酶的浓度对杀螟丹转化率和产品收率的影响，要获得最高的产品收率（4.1 毫克/毫升），最佳温度为 40 °C，杀螟丹浓度为 5 毫克/毫升，漆酶浓度为 0.5 毫克/毫升。合成的低聚物显示出了比氟啶嗪更强的抗氧化活性，而且在广泛的测试浓度范围内对机会致病菌金黄色葡萄球菌有抑制作用，对共生菌表皮葡萄球菌有刺激作用，因此具有很好的用作皮肤益生元的潜力。在表皮葡萄球菌和金黄色葡萄球菌的联合培养中，0.0195 毫克/毫升的浓度表现出最高的益生能力。

{"title":"Laccase-mediated synthesis of phloridzin oligomers: An insight into the influence of reaction conditions and evaluation of oligomers’ biological activity","authors":"Ana Vukoičić , Ana Milivojević , Ljiljana Tolić Stojadinović , Svetlana Grujić , Ljubodrag Vujisić , Vele Tešević , Dejan Bezbradica","doi":"10.1016/j.procbio.2024.11.021","DOIUrl":"10.1016/j.procbio.2024.11.021","url":null,"abstract":"<div><div>The effect of key experimental factors on phloridzin oligomerization catalyzed by <em>Trametes versicolor</em> laccase was investigated, and biological activity of synthesized oligomers was evaluated. Mass spectrometry analysis of the obtained reaction mixture showed the presence of oligomers with the degrees of polymerization two and three with dimer as the major product. The detected masses of the formed oligomers indicated the repetition of phloridzin units in their structure and the loss of two hydrogen atoms during the linkage formation, while NMR analysis of the major dimer confirmed the presence of two phloridzin molecules linked via a C5-C5 bond. The effect of temperature, substrate, and enzyme concentration on phloridzin conversion and products’ yield was examined, and for achieving the highest products yield (4.1 mg/ml), optimal factors were 40 °C temperature, 5 mg/ml phloridzin concentration and 0.5 mg/ml laccase concentration. Synthesized oligomers showed good antioxidant activity compared to phloridzin and very good potential to be used as skin prebiotics since they exhibited inhibitory effect on opportunistic pathogen <em>Staphylococcus aureus</em> and stimulating effect on commensal bacteria <em>Staphylococcus epidermidis</em> in a broad range of tested concentrations. The highest prebiotic capacity was demonstrated at a concentration of 0.0195 mg/ml in <em>S. epidermidis</em> and <em>S. aureus</em> co-culture.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 104-113"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous saccharification and fermentation of municipal cardboard waste for bioethanol production using immobilized cellulases and xylanases onto Cu-MOF 利用固定在 Cu-MOF 上的纤维素酶和木聚糖酶同时对城市纸板废料进行糖化和发酵以生产生物乙醇

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.025

Tanya Kuthiala , Sudarshan Sahu , Gursharan Singh , Madhu Khatri , Shailendra Kumar Arya

This study aims to establish a cost-effective and environmentally friendly method for bioethanol production by optimizing enzymatic hydrolysis of cardboard waste, a significant component of municipal solid waste, in combination with fermentation by Saccharomyces cerevisiae. The use of a cellulases and xylanases enzyme blend, sourced from Thermobifida fusca and Bacillus pumilus, respectively, enhances saccharification efficiency. Pre-treatment with 2 % NaOH results in a substantial 66 % saccharification efficiency at a 5 % solid/substrate loading. Further improvement in hydrolysis efficiency is achieved through enzyme immobilization on Cu-BTC-MOFs, which were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Field Emission Scanning Electron Microscopy (FESEM). The immobilized enzyme blend increases saccharification efficiency to 78 %, compared to 66 % for free enzyme systems. Reusability studies demonstrate a gradual reduction in saccharification efficiency across successive cycles, reaching 71.29 % by the twelfth cycle. When applied in a simultaneous saccharification and fermentation (SSF) setup under optimized conditions, the system yields a notable bioethanol production with a yield of 0.55 g/g and a productivity of 0.20 g/L·h. This study offers potential applications in sustainable bioethanol production and waste valorization by enhancing enzymatic hydrolysis and fermentation of municipal cardboard waste. The approach demonstrates promise for scaling bioenergy production and contributes to addressing waste management challenges, supporting green energy initiatives.

本研究旨在通过优化城市固体废弃物的重要组成部分--纸板废弃物的酶水解，结合酿酒酵母的发酵，建立一种具有成本效益且环保的生物乙醇生产方法。使用纤维素酶和木聚糖酶混合酶（分别来自热纤维菌和枯草芽孢杆菌）可提高糖化效率。用 2% 的 NaOH 进行预处理后，在固体/基质负荷为 5% 的情况下，糖化效率大幅提高了 66%。通过傅立叶变换红外光谱（FTIR）、X 射线衍射（XRD）和场发射扫描电子显微镜（FESEM）对铜-BTC-MOFs 进行表征，进一步提高了水解效率。与游离酶系统的 66% 相比，固定化混合酶将糖化效率提高到 78%。可重复使用性研究表明，糖化效率在连续循环中逐渐降低，到第十二个循环时达到 71.29%。在优化条件下，将该系统应用于同步糖化和发酵（SSF）装置时，生物乙醇产量显著提高，达到 0.55 克/克，生产率为 0.20 克/升-小时。这项研究通过加强城市纸板废物的酶水解和发酵，为可持续生物乙醇生产和废物价值化提供了潜在的应用前景。该方法展示了扩大生物能源生产规模的前景，有助于应对废物管理挑战，支持绿色能源倡议。

{"title":"Simultaneous saccharification and fermentation of municipal cardboard waste for bioethanol production using immobilized cellulases and xylanases onto Cu-MOF","authors":"Tanya Kuthiala , Sudarshan Sahu , Gursharan Singh , Madhu Khatri , Shailendra Kumar Arya","doi":"10.1016/j.procbio.2024.11.025","DOIUrl":"10.1016/j.procbio.2024.11.025","url":null,"abstract":"<div><div>This study aims to establish a cost-effective and environmentally friendly method for bioethanol production by optimizing enzymatic hydrolysis of cardboard waste, a significant component of municipal solid waste, in combination with fermentation by <em>Saccharomyces cerevisiae</em>. The use of a cellulases and xylanases enzyme blend, sourced from <em>Thermobifida fusca</em> and <em>Bacillus pumilus</em>, respectively, enhances saccharification efficiency. Pre-treatment with 2 % NaOH results in a substantial 66 % saccharification efficiency at a 5 % solid/substrate loading. Further improvement in hydrolysis efficiency is achieved through enzyme immobilization on Cu-BTC-MOFs, which were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Field Emission Scanning Electron Microscopy (FESEM). The immobilized enzyme blend increases saccharification efficiency to 78 %, compared to 66 % for free enzyme systems. Reusability studies demonstrate a gradual reduction in saccharification efficiency across successive cycles, reaching 71.29 % by the twelfth cycle. When applied in a simultaneous saccharification and fermentation (SSF) setup under optimized conditions, the system yields a notable bioethanol production with a yield of 0.55 g/g and a productivity of 0.20 g/L·h. This study offers potential applications in sustainable bioethanol production and waste valorization by enhancing enzymatic hydrolysis and fermentation of municipal cardboard waste. The approach demonstrates promise for scaling bioenergy production and contributes to addressing waste management challenges, supporting green energy initiatives.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 114-123"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linguistics of microbiota – Quorum sensing in anaerobic digestion, biochemistry, characteristics, and perspectives of future research – A mini-review 微生物群的语言学 - 厌氧消化中的法定人数感应、生物化学、特点和未来研究展望 - 小综述

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.procbio.2024.11.026

Michał Kozłowski , Krzysztof Marycz , Andrzej Białowiec

This manuscript discusses the mechanism of quorum sensing, provides a brief description of the phenomenon, biochemistry properties, and quorum quenching mechanism, and provides a perspective for future research with some implications regarding its use in industry. Particular emphasis was placed on using these signal molecules in anaerobic digestion. A proper understanding of the processes occurring during methane fermentation could be crucial to in the prospects of increasing biogas production. The types of signaling molecules vary between microorganisms, and the way they are synthesized or secreted (active or passive) differs. This type of communication could induce biofilm formation, bioluminescence, metabolite synthesis, motility, and conjugation. Appropriate knowledge and understanding of the operation of signaling molecules might be essential to increasing the efficiency of anaerobic digestion processes. The phenomenon of quorum sensing itself can be successfully used in medicine, renewable energy (biogas plants), or the chemical industry.

本手稿讨论了法定人数感应的机制，简要介绍了这一现象、生物化学特性和法定人数淬灭机制，并对未来的研究提出了展望，同时对其在工业中的应用提出了一些建议。特别强调了在厌氧消化中使用这些信号分子。正确理解甲烷发酵过程对提高沼气产量至关重要。不同微生物的信号分子类型不同，合成或分泌（主动或被动）的方式也不同。这类交流可诱导生物膜形成、生物发光、代谢物合成、运动和共轭。适当了解和掌握信号分子的运作对提高厌氧消化过程的效率至关重要。法定人数感应现象本身可成功应用于医学、可再生能源（沼气厂）或化学工业。

{"title":"Linguistics of microbiota – Quorum sensing in anaerobic digestion, biochemistry, characteristics, and perspectives of future research – A mini-review","authors":"Michał Kozłowski , Krzysztof Marycz , Andrzej Białowiec","doi":"10.1016/j.procbio.2024.11.026","DOIUrl":"10.1016/j.procbio.2024.11.026","url":null,"abstract":"<div><div>This manuscript discusses the mechanism of quorum sensing, provides a brief description of the phenomenon, biochemistry properties, and quorum quenching mechanism, and provides a perspective for future research with some implications regarding its use in industry. Particular emphasis was placed on using these signal molecules in anaerobic digestion. A proper understanding of the processes occurring during methane fermentation could be crucial to in the prospects of increasing biogas production. The types of signaling molecules vary between microorganisms, and the way they are synthesized or secreted (active or passive) differs. This type of communication could induce biofilm formation, bioluminescence, metabolite synthesis, motility, and conjugation. Appropriate knowledge and understanding of the operation of signaling molecules might be essential to increasing the efficiency of anaerobic digestion processes. The phenomenon of quorum sensing itself can be successfully used in medicine, renewable energy (biogas plants), or the chemical industry.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 183-190"},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing riboflavin production by modulating the expression of the rib operon in Bacillus subtilis 通过调节枯草芽孢杆菌核糖操作子的表达提高核黄素产量

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-11-17 DOI: 10.1016/j.procbio.2024.11.020

Mengyu Yao , Miaomiao Xia , Jinlong Li , Huina Dong , Liping Du , Dawei Zhang

Riboflavin is an essential vitamin for humans and animals, with its annual demand experiencing a consistent upward trajectory. In recent years, newly constructed riboflavin producing strains are difficult to reach the level of industrial strains. Therefore, developing competitive strains is crucial for advancing the riboflavin industry. The expression level of the rib operon is strongly correlated with riboflavin biosynthesis. The strain S12, which overexpressed the rib operon of B. subtilis using plasmids with approximately 8 copies, achieved a 53.9 % increase in riboflavin biosynthesis during shake-flask fermentation. However, the strain cannot grow normally in a 7.5-L bioreactor. The expression of rib operon may have competed with cell growth for GTP or produced toxic intermediate ArP. Therefore, the strain S16 replaced ribA in rib operon with a monofunctional DHBP synthase, increased 20 % of riboflavicn production in a 7.5-L bioreactor. Furthermore, the strain S24, which overexpressed the rib operon using the phase-dependent promoter P_yqgZ, attained a riboflavin production of 29 g/L in a 7.5-L bioreactor, achieving the highest reported riboflavin production to date. This study reports for the first time using the phase-dependent promoter for overexpression of rib operon, effectively regulated the relationship between cell growth and riboflavin production.

核黄素是人类和动物必需的维生素，每年的需求量呈持续上升趋势。近年来，新建的核黄素生产菌株很难达到工业化菌株的水平。因此，开发具有竞争力的菌株对于推动核黄素产业的发展至关重要。核糖操作子的表达水平与核黄素的生物合成密切相关。利用约 8 个拷贝的质粒过量表达枯草芽孢杆菌核糖操作子的菌株 S12，在摇瓶发酵过程中核黄素生物合成量增加了 53.9%。然而，该菌株无法在 7.5 升生物反应器中正常生长。核糖操作子的表达可能与细胞生长竞争 GTP 或产生有毒的中间产物 ArP。因此，菌株 S16 用单功能 DHBP 合成酶取代了核糖操作子中的核糖 A，使 7.5 升生物反应器中核黄素的产量提高了 20%。此外，利用相依赖启动子 PyqgZ 过表达核糖操作子的菌株 S24 在 7.5 升生物反应器中的核黄素产量达到了 29 克/升，实现了迄今为止报道的最高核黄素产量。该研究首次报道了利用相位依赖性启动子过量表达核糖操作子，有效调节了细胞生长与核黄素产量之间的关系。

{"title":"Enhancing riboflavin production by modulating the expression of the rib operon in Bacillus subtilis","authors":"Mengyu Yao , Miaomiao Xia , Jinlong Li , Huina Dong , Liping Du , Dawei Zhang","doi":"10.1016/j.procbio.2024.11.020","DOIUrl":"10.1016/j.procbio.2024.11.020","url":null,"abstract":"<div><div>Riboflavin is an essential vitamin for humans and animals, with its annual demand experiencing a consistent upward trajectory. In recent years, newly constructed riboflavin producing strains are difficult to reach the level of industrial strains. Therefore, developing competitive strains is crucial for advancing the riboflavin industry. The expression level of the <em>rib</em> operon is strongly correlated with riboflavin biosynthesis. The strain S12, which overexpressed the <em>rib</em> operon of <em>B. subtilis</em> using plasmids with approximately 8 copies, achieved a 53.9 % increase in riboflavin biosynthesis during shake-flask fermentation. However, the strain cannot grow normally in a 7.5-L bioreactor. The expression of <em>rib</em> operon may have competed with cell growth for GTP or produced toxic intermediate ArP. Therefore, the strain S16 replaced <em>ribA</em> in <em>rib</em> operon with a monofunctional DHBP synthase, increased 20 % of riboflavicn production in a 7.5-L bioreactor. Furthermore, the strain S24, which overexpressed the <em>rib</em> operon using the phase-dependent promoter P<sub><em>yqgZ</em></sub>, attained a riboflavin production of 29 g/L in a 7.5-L bioreactor, achieving the highest reported riboflavin production to date. This study reports for the first time using the phase-dependent promoter for overexpression of <em>rib</em> operon, effectively regulated the relationship between cell growth and riboflavin production.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 139-149"},"PeriodicalIF":3.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0