Reproductive biology最新文献_第2页

Annatto concentrate supplementation improves systemic redox balance and modulates placental FGF-2 expression in ewes 母羊补充红木精料可改善全身氧化还原平衡，调节胎盘FGF-2表达

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-11-13 DOI: 10.1016/j.repbio.2025.101099

Leonardo Lima Salata , Calie Castilho , Isabella Guartieri da Silva , Leticia Jalloul Guimarães , Isabella Pereira Azoia , Ines Cristina Giometti , Dionísio Pedro Lopes Kuhn Pinheiro , Alexandre Martins Olivete , Marilice Zundt

Annatto (Bixa orellana) carotenoids have antioxidant potential, but effects on placental biology and antioxidant parameters in ewes are unclear. We tested whether dietary annatto concentrate (ANC) in late gestation modulates immediate-postpartum blood oxidative markers and placental gene abundance. Thirty ewes were stratified by expected litter size (single/twin) and randomized within stratum to 0 %, 0.5 %, or 1.0 % ANC (dry-matter basis) from gestational day 100. ANC was a powdered concentrate, premixed into feed (1.5 % BW) and offered individually to ensure full intake. At lambing, we recorded maternal/neonatal descriptors (ewe weights, gestational length, time to placental expulsion, and lamb birth weight interpreted with litter size and sex), collected blood for FRAP, TBARS–MDA, H₂O₂, SOD, GPx, CAT, and –SH, and sampled placenta (cotyledon and intercotyledonary tissue) aseptically and froze it for RT-qPCR of VEGF-A, FGF-2, PAG1, GPX1, GSR, CAT, and SOD2. ANC produced marker-specific blood adjustments: lower H₂O₂ at 1.0 %, higher GPx in supplemented ewes, and lower –SH at 0.5 %, whereas FRAP, SOD, MDA, and CAT were unchanged. In placenta, cotyledonary FGF-2 showed higher relative abundance at 0.5 %, with no differences for VEGF-A, PAG1, GPX1, GSR, CAT, or SOD2 in either tissue. Ewe weights, gestational length, and time to placental expulsion were similar among groups; lamb birth weight showed no main effect of ANC. These results indicate that, in late gestation, ANC selectively increased the relative abundance of cotyledonary FGF-2 at 0.5 % and modulated peroxide handling in blood, supporting its use as a nutritional tool for antioxidant support near parturition.

红木（Bixa orellana）类胡萝卜素具有抗氧化潜力，但对母羊胎盘生物学和抗氧化参数的影响尚不清楚。我们测试了妊娠后期饮食中的红木浓缩物（ANC）是否会调节产后立即血液氧化标志物和胎盘基因丰度。30只母羊按预期产仔数（单/双）分层，从妊娠第100天开始按0 %、0.5 %和1.0 %的ANC（干物质基础）分层。ANC是一种粉末状浓缩物，预混在饲料中（1.5 %体重），单独提供以确保全采食量。在羔羊期，我们记录了母羊/新生儿描述因子（母羊体重、妊娠期长度、胎盘排出时间和羔羊出生体重与产仔数和性别解释），采集血液中FRAP、TBARS-MDA、H₂O₂、SOD、GPx、CAT和-SH的含量，并无菌取样胎盘（子叶和子叶间组织），冷冻后进行VEGF-A、FGF-2、PAG1、GPX1、GSR、CAT和SOD2的RT-qPCR检测。ANC产生了标记特异性的血液调节：补充的母羊的h2o2降低到1.0 %，GPx升高，-SH降低到0.5 %，而FRAP、SOD、MDA和CAT不变。在胎盘中，子叶中FGF-2的相对丰度较高，为0.5 %，而VEGF-A、PAG1、GPX1、GSR、CAT或SOD2在两种组织中均无差异。母羊体重、妊娠期长度和胎盘排出时间各组间相似；ANC对羔羊出生重无主要影响。这些结果表明，在妊娠后期，ANC选择性地增加子叶FGF-2的相对丰度（0.5 %），并调节血液中过氧化物的处理，支持其作为分娩前抗氧化支持的营养工具的作用。

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引用次数: 0

Non-surgical sterilization of male animals using sclerosing agents: A systematic review of intratesticular and intraepididymal injection protocols 雄性动物使用硬化剂的非手术绝育：睾丸内和附睾内注射方案的系统回顾。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-11-11 DOI: 10.1016/j.repbio.2025.101101

Iara Magalhães Ribeiro , Renner Philipe Rodrigues Carvalho , Arabela Guedes de Azevedo Viana , Camilo Jose Ramirez-Lopez , Tayná Bolsam da Silva , Mariana Machado-Neves

The overpopulation of free-roaming animals, particularly dogs and cats, poses a significant global public health concern, contributing to socioeconomic, ecological, political, and ethical issues. These animals reproduce freely, increasing the risk of zoonotic disease transmission, wildlife predation, and traffic accidents. Although surgical castration is the most widely used method for controlling the male animal population, it is costly, time-consuming, and requires specialized professionals and post-operative care. Therefore, there is an urgent need for practical, low-cost, large-scale, and welfare-friendly non-surgical sterilization methods. This systematic review aimed to evaluate the efficacy of intratesticular or intraepididymal injections of sclerosing agents for inducing permanent chemical sterilization. A total of 38 peer-reviewed articles were retrieved from Medline/PubMed, Web of Science, Embase, and Scopus. Among sixteen substances, calcium chloride was the most frequently studied, followed by zinc gluconate, hypertonic saline, chlorhexidine gluconate, mannitol, and glycerol. A single bilateral intratesticular and intraepididymal injection damaged testicular and epididymal tissues, thereby disrupting spermatogenesis, steroidogenesis, and sperm transport. Despite differences in the methodology and outcomes among studies, most tested agents showed promising results in disrupting reproductive function. None of the compounds fully met the two key criteria for their practical use, including the absence of side effects and consistent induction of irreversible azoospermia or infertility. Nevertheless, calcium chloride and hypertonic saline emerged as the most promising candidates, due to their robust mechanistic profiles, broad evidence base, and favorable cost-effectiveness. Therefore, researchers may prioritize these two agents in future studies to develop standardized, safe, and field-applicable protocols for large-scale population control.

自由漫游的动物，特别是狗和猫的数量过剩，引起了重大的全球公共卫生问题，并导致了社会经济、生态、政治和伦理问题。这些动物自由繁殖，增加了人畜共患疾病传播、野生动物捕食和交通事故的风险。尽管手术阉割是控制雄性动物数量最广泛使用的方法，但它成本高，耗时长，需要专业的专业人员和术后护理。因此，迫切需要一种实用、低成本、规模化、福利友好的非手术绝育方法。本系统综述旨在评价睾丸内或附睾内注射硬化剂诱导永久性化学灭菌的疗效。共从Medline/PubMed、Web of Science、Embase和Scopus检索了38篇同行评议文章。在16种物质中，氯化钙是最常被研究的，其次是葡萄糖酸锌、高渗盐水、葡萄糖酸氯己定、甘露醇和甘油。单次双侧睾丸内和附睾内注射损伤睾丸和附睾组织，从而破坏精子发生、甾体发生和精子运输。尽管研究的方法和结果不同，但大多数测试的药物在破坏生殖功能方面显示出有希望的结果。没有一种化合物完全符合其实际使用的两个关键标准，包括没有副作用和持续诱导不可逆的无精子症或不孕症。然而，氯化钙和高渗盐水因其强大的机制特征、广泛的证据基础和良好的成本效益而成为最有希望的候选者。因此，研究人员可以在未来的研究中优先考虑这两种药物，以制定标准化、安全、现场适用的大规模种群控制方案。

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引用次数: 0

Inhibitory role of activin A during luteinization of follicular and lutein granulosa cells from small antral and preovulatory human follicles 激活素A在卵泡黄体化和卵泡叶黄素颗粒细胞中的抑制作用。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-11-29 DOI: 10.1016/j.repbio.2025.101169

Lea Bejstrup Jensen , Laura Victoria Højer , Lisette Schönhage , Cristina Subiran Adrados , Jane Alrø Bøtkjær , Tanni Borgbo , Jesús Cadenas , Kirsten Tryde Macklon , Stine Ringholm , Anette Tønnes Pedersen , Stine Gry Kristensen

Luteinization is a vital process in female reproduction, where granulosa cells differentiate into progesterone-secreting luteal cells. While luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are established promoters of luteinization, the regulatory role of activin A remains incompletely understood. This study aimed to investigate whether activin A can prevent or reverse luteinization in granulosa cells from small antral and preovulatory follicles using an in vitro model. Granulosa cells were collected from women undergoing either IVF treatment or ovarian tissue cryopreservation. Cells were cultured for 48 h with FSH, hCG, or activin A. Hormone secretion (progesterone and estradiol) and content of steroidogenic markers (CYP19A1, STAR, 17βHSD1, 3βHSD2, FSHR, and LHCGR) were analyzed via ELISA, RT-qPCR, and Western blot.

Results

demonstrated that activin A suppressed luteinization markers and progesterone production while promoting estrogen synthesis and maintaining granulosa cell features. Conversely, hCG increased luteinization marker expression and progesterone secretion, consistent with its known role in luteal transformation. Notably, granulosa cells from small antral follicles showed a distinct, stage-specific response to activin A compared to those from preovulatory follicles, indicating a temporal regulation of luteinization competence.

These findings support a regulatory role for activin A in maintaining granulosa cell identity and delaying luteinization. Understanding the differential responsiveness of granulosa cells across follicular development stages may inform new therapeutic approaches for luteal phase deficiencies and fertility preservation strategies.

黄体生成是女性生殖过程中一个重要的过程，其中颗粒细胞分化为分泌黄体激素的黄体细胞。虽然黄体生成素（LH）和人绒毛膜促性腺激素（hCG）是已知的黄体生成素的促进因子，但活化素A的调节作用仍不完全清楚。本研究旨在通过体外模型研究激活素A是否能阻止或逆转小窦卵泡和排卵前卵泡颗粒细胞的黄体生成化。颗粒细胞从接受体外受精治疗或卵巢组织冷冻保存的妇女中收集。用FSH、hCG或activin a培养细胞48 h，通过ELISA、RT-qPCR和Western blot分析激素分泌（孕酮和雌二醇）和激素生成标志物（CYP19A1、STAR、17βHSD1、3βHSD2、FSHR和LHCGR）的含量。结果：激活素A抑制黄体生成素标记物和黄体酮的产生，同时促进雌激素合成，维持颗粒细胞特征。相反，hCG增加了黄体生成素标记物的表达和黄体酮的分泌，这与它在黄体转化中的已知作用一致。值得注意的是，与来自排卵前卵泡的颗粒细胞相比，来自小腔卵泡的颗粒细胞对激活素a表现出明显的阶段特异性反应，表明黄体生成素能力的时间调节。这些发现支持激活素a在维持颗粒细胞身份和延缓黄体生成中的调节作用。了解颗粒细胞在卵泡发育阶段的不同反应性可能为黄体期缺陷和生育保护策略提供新的治疗方法。

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引用次数: 0

Endometrial immune profile: A predictor of pregnancy success 子宫内膜免疫特征：妊娠成功的预测因子

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-12-31 DOI: 10.1016/j.repbio.2025.101174

Sina Baharaghdam , Shima Karimi , Mohammad Esfini Farahani , Leili Aghebati-Maleki , Mehdi Yousefi

The likelihood of a successful pregnancy is influenced by a set of variables that influence endometrial receptivity, including hormonal, genetic, metabolic, age, lifestyle, and immunological factors. Among these, the endometrial immune profile has received particular attention as a critical actor of implantation and embryo tolerance. Dynamic fluctuations in immune cell populations—such as macrophages, dendritic cells, uterine natural killer cells, and regulatory T cells—across the menstrual cycle might significantly affect the endometrium's capacity to support successful implantation. Recent evidence highlights that disruptions in the quantity, phenotype, or function of these immune cells contribute to impaired endometrial receptivity in infertility-related disorders, including recurrent implantation failure, recurrent pregnancy loss, endometriosis, and polycystic ovary syndrome. This review provides a comprehensive assessment of immune cell composition and function in the healthy endometrium compared to pathological conditions, emphasizing how immune dysregulation may impair pregnancy. Furthermore, we evaluate both current and emerging diagnostic modalities—from immunohistochemistry and flow cytometry to high-resolution single-cell transcriptomics—and endometrial microbiome impact on immune profiling that enables more precise characterization of immune profile dysregulation, alongside established and investigational therapies, with particular attention to their efficacy and mechanistic rationale. By integrating these insights, a clinically oriented framework can be provided to guide the development of personalized diagnostic algorithms and targeted immune-based therapies that, ultimately, could lead to improved implantation rates and live birth outcomes in individuals facing infertility disorders with immunological causes.

成功怀孕的可能性受到一系列影响子宫内膜容受性的变量的影响，包括激素、遗传、代谢、年龄、生活方式和免疫因素。其中，子宫内膜免疫谱作为植入和胚胎耐受的关键因素受到了特别的关注。免疫细胞群（如巨噬细胞、树突状细胞、子宫自然杀伤细胞和调节性T细胞）在月经周期中的动态波动可能会显著影响子宫内膜支持成功植入的能力。最近的证据强调，这些免疫细胞的数量、表型或功能的破坏有助于不孕相关疾病的子宫内膜容受性受损，包括反复植入失败、反复妊娠丢失、子宫内膜异位症和多囊卵巢综合征。这篇综述提供了健康子宫内膜中免疫细胞组成和功能的综合评估，并与病理状况进行了比较，强调了免疫失调如何损害妊娠。此外，我们评估了当前和新兴的诊断模式-从免疫组织化学和流式细胞术到高分辨率单细胞转录组学-子宫内膜微生物组对免疫谱的影响，能够更精确地表征免疫谱失调，以及已建立和正在研究的治疗方法，特别关注其疗效和机制原理。通过整合这些见解，可以提供一个临床导向的框架来指导个性化诊断算法和靶向免疫治疗的发展，最终可以提高因免疫原因导致的不孕症患者的着床率和活产结果。

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引用次数: 0

Reproductive biology characterization of violinha Loricariichthys anus 喉喉小提琴的生殖生物学特征

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-12-20 DOI: 10.1016/j.repbio.2025.101171

Douglas C. Selle , Raquel S. dos Santos , Thaiza R. de Freitas , Jhony L. Benato , Thales de S. França , Renata V. Dantas , Marco A. Rotta , Marcelle F.O. Barbosa , Vanessa Coimbra , Diógenes H. Siqueira-Silva , Danilo P. Streit Jr.

This study aimed to characterize the reproductive biology of Loricariichthys anus (violinha) from the Guaíba River (Brazil) to support conservation efforts and development of captive reproduction protocols. Monthly collections (November 2021-October 2022) of 144 specimens were analyzed for sexual dimorphism, gonadosomatic index (GSI), gonadal histology, and sperm parameters (motility, concentration, morphology). Sexual dimorphism was evidenced by elongated lower lip in males, predominantly during the reproductive period (November-March). GSI values peaked during November-March for both sexes (P < 0.05). Histological analysis revealed females reached maturity from November to February with evidence of multiple spawning, while males produced sperm year-round, albeit in low volumes. Sperm motility parameters differed significantly between individuals (P < 0.05) with mean concentration of 6.88 × 106 ± 8.18 × 105 spermatozoa/mL. L. anus reproductive period occurs from November to February, with males presenting low seminal volume and viable sperm concentration compared to other Loricariidae species, which has implications for both conservation and captive reproduction efforts.

本研究旨在研究巴西Guaíba河Loricariichthys anus （violinha）的生殖生物学特征，以支持圈养繁殖方案的制定和保护工作。对每月采集的144例标本（2021年11月至2022年10月）进行两性二态性、性腺指数（GSI）、性腺组织学和精子参数（活力、浓度、形态）的分析。雌雄二态性表现为雄性下唇拉长，主要发生在生殖期（11 - 3月）。GSI值在11月至3月期间达到峰值（P <； 0.05）。组织学分析显示，雌性从11月到2月达到成熟，有证据表明多次产卵，而雄性全年产生精子，尽管数量较少。精子活力指标个体间差异显著（P <； 0.05），平均浓度为6.88 × 106 ± 8.18 × 105个精子/mL。在11月至次年2月的生殖期，与其他蠓科物种相比，雄性蠓的精液量和存活精子浓度较低，这对保护和圈养繁殖具有重要意义。

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引用次数: 0

Prediction of euploidy and probability of obtaining target number of euploid embryos through preimplantation genetic testing for aneuploidy 通过着床前非整倍体基因检测预测整倍体及获得目标数量整倍体胚胎的概率

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-11-20 DOI: 10.1016/j.repbio.2025.101104

Kazuma Onishi , Daichi Inoue , Yuta Kida , Masae Kojima , Chiharu Ishida , Takahiro Suzuki , Shuhei Kamada , Noritaka Fukunaga , Yoshimasa Asada

We aimed to develop prediction models estimating (1) the probability of obtaining a target number of euploid embryos per individual and (2) the probability of euploidy at the embryo level using data from preimplantation genetic testing for aneuploidy (PGT-A). This retrospective observational study included 664 individuals who underwent PGT-A between March 2020 and October 2024, totaling 5539 biopsied embryos (23.2 % euploid). Among them, 153 had no euploid embryos and 511 had at least one. Four models were developed: Models 1-A, 1-B, and 1-C predicted the probability of obtaining ≥ 1, ≥ 2, and ≥ 3 euploid embryos, respectively. Model 2 predicted the probability of euploidy per embryo. Multivariate logistic regression was used for individual-level models (1-A to 1-C), and a generalized estimating equation was applied for Model 2. Predictors included maternal age at retrieval, number of biopsied embryos, and embryo morphology. Area under the receiver operating characteristic curve (AUC) was used to examine the discriminative ability of models. Internal validation was performed using 10-fold cross-validation. Decision curve analysis assessed clinical utility. Models 1-A, 1-B, and 1-C demonstrated good discrimination (AUCs: 0.85–0.87), while Model 2 showed fair discrimination (AUC: 0.77). Decision curve analysis supported the clinical utility of all models at wide range of thresholds probabilties. The individual-level prediction models demonstrated good discriminative ability, while the embryo-level model showed fair discriminative ability. All models demonstrated potential clinical utility in supporting embryo selection.

我们的目的是建立预测模型来估计(1)每个个体获得目标数量的整倍体胚胎的概率和(2)胚胎水平上整倍体的概率，利用着床前非整倍体基因检测（PGT-A）的数据。这项回顾性观察性研究包括664名在2020年3月至2024年10月期间接受PGT-A的个体，共5539个活检胚胎（23.2% %整倍体）。其中，153只没有整倍体胚胎，511只至少有一个整倍体胚胎。建立了四种模型：模型1- a、1- b和1- c分别预测获得≥ 1、≥ 2和≥ 3个整倍体胚胎的概率。模型2预测了每个胚胎整倍性的概率。个体水平模型（1-A至1-C）采用多元逻辑回归，模型2采用广义估计方程。预测因子包括母亲取卵时的年龄、活检胚胎的数量和胚胎形态。采用受试者工作特征曲线下面积（AUC）来检验模型的判别能力。采用10倍交叉验证进行内部验证。决策曲线分析评估临床效用。模型1-A、1-B和1-C具有良好的判别性（AUC: 0.85 ~ 0.87），而模型2具有一般的判别性（AUC: 0.77）。决策曲线分析支持所有模型在大范围阈值概率下的临床应用。个体水平预测模型具有较好的判别能力，胚胎水平预测模型具有一般的判别能力。所有模型在支持胚胎选择方面都显示出潜在的临床效用。

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引用次数: 0

Moringa oleifera leaves aqueous extract mitigates nicotine-induced reproductive toxicity in male rats 辣木叶水提物减轻尼古丁诱导的雄性大鼠生殖毒性

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-10-15 DOI: 10.1016/j.repbio.2025.101093

Ahmed Mohamed Ibrahim, Ahmed Emam Dakrory, Amel Ramadan Omar , Aya A. Mahmoud

Nicotine, a chemical component included in tobacco, has been demonstrated to influence various facets of spermatogenesis and sperm functionality. Moringa oleifera Lam. (Moringaceae) is used in traditional medicine to treat various ailments, including reproductive toxicity. The current study aims to investigate the ameliorative effect of Moringa oleifera leaves aqueous extract (MOE) on nicotine (NT)-induced reproductive toxicity in male rats. Forty rats were assigned into four groups (n = 10): control (distilled water), MOE (400 mg/kg), NT (1 mg/kg), and NT + MOE groups, and orally treated for 56 days. At the end of the experiment, body and organ weights, epididymal sperm analysis, oxidative stress, hormone levels, and histopathological abnormalities were assessed. Nicotine administration results in a significant increase in malondialdehyde (MDA) levels, accompanied by a decrease in glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD). Consequently, NT decreases sperm quality and serum reproductive hormone levels, including testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH), and distorts testicular tissue architecture. The oral administration of MOE caused a significant decrease in MDA concentration and an increase in the levels of antioxidant molecules (GSH, CAT, SOD) and serum reproductive hormones levels. Additionally, it improved sperm characteristics, including sperm count, motility, viability, and morphology and partial improvement to the histological architecture of the testis. Our findings imply that 400 mg/kg of Moringa oleifera leaves may be utilized to enhance reproductive parameters against NT-induced reproductive toxicity in male rats, with the primary mechanism attributed to its antioxidant properties.

尼古丁是烟草中含有的一种化学成分，已被证明可以影响精子发生和精子功能的各个方面。辣木。（辣木科）在传统医学中用于治疗各种疾病，包括生殖毒性。本研究旨在探讨辣木叶水提物（MOE）对尼古丁（NT）诱导的雄性大鼠生殖毒性的改善作用。将40只大鼠分为4组（n = 10）：对照组（蒸馏水）、MOE组（400 mg/kg）、NT组（1 mg/kg）、NT + MOE组，口服56 d。实验结束时，对大鼠的体重、脏器重量、附睾精子分析、氧化应激、激素水平和组织病理学异常进行评估。服用尼古丁会导致丙二醛（MDA）水平显著增加，同时伴有谷胱甘肽（GSH）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的减少。因此，NT降低精子质量和血清生殖激素水平，包括睾酮、促卵泡激素（FSH）和黄体生成素（LH），并扭曲睾丸组织结构。口服MOE可显著降低MDA浓度，升高抗氧化分子（GSH、CAT、SOD）水平和血清生殖激素水平。此外，它还改善了精子特征，包括精子数量、活力、活力和形态，并部分改善了睾丸的组织学结构。我们的研究结果表明，400 mg/kg辣木叶可用于增强雄性大鼠的生殖参数，以对抗nt诱导的生殖毒性，其主要机制归因于其抗氧化特性。

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引用次数: 0

USP13 exacerbates the malignant progression of cervical cancer by inhibiting ECT2 ubiquitination and degradation USP13通过抑制ECT2泛素化和降解而加剧宫颈癌的恶性进展。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-11-11 DOI: 10.1016/j.repbio.2025.101095

Tingting Li , Xiulan Xiong , Tingting Xie , Wei Liu , Xuqin Feng , Silin Chen , Xin Hu , Rengui Li , Kaiwen Fu

Cervical cancer (CC) is one of the most common malignant tumors affecting the female reproductive system. Epithelial Cell Transforming Sequence 2 (ECT2), a guanine nucleotide exchange factor, is crucial in regulating cellular functions. This study focuses on elucidating the role of ECT2 in CC and the involved underlying mechanisms. Western blot verified protein expression in tissues and cells. Cell proliferation, migration, and invasion were assessed via clone formation, wound healing, and transwell assays. Glycolytic indicators (glucose uptake, lactate release, ATP levels, lactate dehydrogenase (LDH) activity, and pyruvate dehydrogenase (PDH) activity) were detected using specific kits. UbiBrowser was used to predict ubiquitin-specific protease 13 (USP13)-mediated ECT2 deubiquitination. Co-immunoprecipitation (Co-IP) was performed to validate USP13’s deubiquitination on ECT2 and their interaction. In vivo validation employed a mouse xenograft model, with immunohistochemistry (IHC) assessing gene expression therein. This study demonstrated that ECT2 was upregulated in CC tissues and cells, and its downregulation effectively inhibited the proliferation, migration, invasion, and glycolysis of CC cells. USP13 exhibited high expression levels in CC and stabilized ECT2 expression through its deubiquitinating activity. Knockdown of USP13 significantly suppressed the malignant phenotypes of CC cells; however, this suppressive effect was markedly reversed upon ECT2 overexpression. In vivo experiments revealed that USP13 knockdown suppressed CC tumor growth by modulating ECT2 expression. Together, USP13 exacerbates the malignant progression of CC by inhibiting ECT2 ubiquitination, suggesting that targeting the USP13-ECT2 axis might be a potential therapeutic strategy for CC with notable clinical significance.

宫颈癌（CC）是影响女性生殖系统最常见的恶性肿瘤之一。上皮细胞转化序列2 （ECT2）是一种鸟嘌呤核苷酸交换因子，在调节细胞功能中起着至关重要的作用。本研究的重点是阐明ECT2在CC中的作用及其潜在机制。Western blot证实蛋白在组织和细胞中的表达。通过克隆形成、伤口愈合和transwell试验评估细胞增殖、迁移和侵袭。使用特异性试剂盒检测糖酵解指标（葡萄糖摄取、乳酸释放、ATP水平、乳酸脱氢酶（LDH）活性和丙酮酸脱氢酶（PDH）活性）。UbiBrowser用于预测泛素特异性蛋白酶13 （USP13）介导的ECT2去泛素化。采用免疫共沉淀法（Co-IP）验证USP13对ECT2的去泛素化作用及其相互作用。体内验证采用小鼠异种移植模型，免疫组织化学（IHC）评估其中的基因表达。本研究表明，ECT2在CC组织和细胞中表达上调，其下调可有效抑制CC细胞的增殖、迁移、侵袭和糖酵解。USP13在CC中表现出高表达水平，并通过其去泛素化活性稳定了ECT2的表达。敲低USP13可显著抑制CC细胞的恶性表型；然而，这种抑制作用在ECT2过表达时明显逆转。体内实验表明，USP13敲低通过调节ECT2的表达抑制CC肿瘤的生长。综上所述，USP13通过抑制ECT2泛素化而加剧CC的恶性进展，提示靶向USP13-ECT2轴可能是一种具有显著临床意义的CC潜在治疗策略。

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引用次数: 0

Emerging role and interplay of programmed cell death in endometritis: Insights into mechanisms and therapeutic targets 程序性细胞死亡在子宫内膜炎中的新作用和相互作用：机制和治疗靶点的见解。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-11-12 DOI: 10.1016/j.repbio.2025.101098

Islam Ahmed Abdelmawgood , Dina M.M. Kotb , Ahmed Ezzat Mostafa , Bahaa Sharaf , Sohaib Ali Ramadan , Seif Isamil , Ali Moataz , Mariam Abdelghany , Donia Mohamed Hussien , Michael Ibrahim Boushra

Endometritis is the inflammation of the endometrial lining, frequently linked to pelvic pain. Endometritis is significantly associated with postpartum problems, including infertility. Programmed cell death (PCD) is a genetically regulated mechanism of autonomic and regulated cell death that preserves homeostasis and promotes development. PCD, which includes apoptosis, pyroptosis, autophagy, ferroptosis, and necroptosis, is essential in the pathogenesis of reproductive diseases, including endometritis. Despite the limited study on PCD in endometritis, it is essential to identify important molecules and comprehend their regulatory functions for effective disease prevention and management. This review delineates the different types of PCD and their interactions in endometritis. It also evaluates the progress in PCD research pertaining to endometritis. The objective is to create a basis for subsequent investigations into the function and interaction of PCD in endometritis. We anticipate that this study will facilitate the exploration of novel PCD targets for endometritis treatment, hence encouraging comprehensive research.

子宫内膜炎是子宫内膜的炎症，通常与盆腔疼痛有关。子宫内膜炎与包括不孕症在内的产后问题密切相关。程序性细胞死亡（PCD）是一种基因调控的细胞自主和受调控的死亡机制，可保持体内平衡并促进发育。PCD包括凋亡、焦亡、自噬、铁亡和坏死亡，在包括子宫内膜炎在内的生殖疾病的发病机制中是必不可少的。尽管PCD在子宫内膜炎中的研究有限，但识别重要分子并了解其调控功能对于有效预防和管理疾病至关重要。本文综述了不同类型的PCD及其在子宫内膜炎中的相互作用。它还评估了与子宫内膜炎有关的PCD研究进展。目的是为后续研究PCD在子宫内膜炎中的功能和相互作用奠定基础。我们期望这项研究将有助于探索新的PCD治疗子宫内膜炎的靶点，从而促进全面的研究。

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引用次数: 0

Exosomal ncRNAs in seminal fluid: Unraveling their regulatory roles in male infertility 精液外泌体ncRNAs：揭示其在男性不育中的调节作用

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2026-03-01 Epub Date: 2025-12-31 DOI: 10.1016/j.repbio.2025.101175

Marwah Suliman Maashi

By transmitting bioactive substances, including proteins, lipids, and a varied spectrum of non-coding RNAs, seminal exosomes—including epididymosomes and prostasomes—are shown to be crucial in sperm maturation, motility, and protection. The paper emphasizes how miRNAs, lncRNAs, and circRNAs within these exosomes affect gene expression during spermatogenesis and are starting to be interesting non-invasive biomarkers for several infertility abnormalities, including azoospermia and asthenozoospermia. It also addresses the therapeutic possibilities of exosome-based approaches in reducing inflammation, oxidative stress, and cellular damage in the male reproductive system. The paper identifies essential information gaps and suggests future research paths to clarify the molecular processes behind male infertility by combining knowledge from proteomic, lipidomic, and transcriptomic investigations. These results help to close the knowledge gap on seminal exosomes and open the door for creative therapeutic and diagnostic tools in reproductive medicine.

通过传递生物活性物质，包括蛋白质、脂质和各种非编码rna，精子外泌体（包括附睾和前列腺体）在精子成熟、运动和保护中起着至关重要的作用。本文强调了这些外泌体中的miRNAs、lncRNAs和circRNAs如何影响精子发生过程中的基因表达，并开始成为几种不孕症异常（包括无精子症和弱精子症）的有趣的非侵入性生物标志物。它还解决了基于外泌体的治疗方法在减少炎症、氧化应激和男性生殖系统细胞损伤方面的可能性。本文确定了必要的信息缺口，并提出了未来的研究路径，以阐明男性不育背后的分子过程，通过结合蛋白质组学，脂质组学和转录组学研究的知识。这些结果有助于缩小对精液外泌体的知识差距，并为生殖医学的创造性治疗和诊断工具打开大门。

引用次数: 0