Reproductive biology最新文献_第2页

Correlation between Polycomb repressive complex proteins and epithelial–mesenchymal transition-associated genes in endometriotic tissues 子宫内膜异位症组织中Polycomb抑制复合体蛋白与上皮-间充质过渡相关基因的相关性

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-19 DOI: 10.1016/j.repbio.2025.101173

Anushree Nandan , Abhishek Mangeshikar , Vaijayanti Kale , Anuradha Vaidya , Prasad Pethe

Endometriosis is a condition in which functional endometrial glands and stroma are found to grow outside the uterine cavity that can lead to symptoms like dysmenorrhea, dyspareunia, adhesions, and infertility. Current treatment strategies can provide only symptomatic relief as its etiology remains unclear. Several studies have linked the cellular process of epithelial–mesenchymal transition (EMT) to endometriosis development; however, what triggers EMT states in endometriosis is unknown. Polycomb group (PcG) proteins are histone modifiers that control gene expression by catalyzing repressive histone modifications such as H3K27me3/2 and H2AK119ub1. The misexpression of PcGs and/or genes controlled by them reportedly causes several types of cancers, such as breast, colon, pancreatic, and liver. We investigated whether dysregulation of PcG proteins such as RING1B, BMI1, and EZH2 in ectopic endometriotic tissue as compared to eutopic correlates with genes required for EMT in endometriotic tissue from the same patient with laparoscopically confirmed endometriosis. We quantified the expression of Polycomb repressive complex 1 (PRC1) genes (RING1B and BMI1), EMT-associated genes (TWIST, SNAI1, SNAI2, ZEB1, CDH1, CDH2, and VIM) in paired eutopic and ectopic (endometriotic) tissue samples obtained from 12 women who underwent laparoscopy for severe endometriosis identified as per #ENZIAN classification. Our results showed that the endometriotic lesions had higher gene expression of PRC1 proteins – RING1B and BMI1 as well as higher expression of EMT associated genes than the endometrial tissue. Thus, our results suggest that PRC1 proteins may have a role in the pathogenesis of endometriosis.

子宫内膜异位症是一种功能正常的子宫内膜腺体和间质生长在子宫腔外的情况，可导致痛经、性交困难、粘连和不孕等症状。目前的治疗策略只能提供症状缓解，因为其病因尚不清楚。一些研究将上皮-间质转化（EMT）的细胞过程与子宫内膜异位症的发展联系起来；然而，是什么触发了子宫内膜异位症的EMT状态尚不清楚。Polycomb group （PcG）蛋白是组蛋白修饰因子，通过催化抑制组蛋白修饰如H3K27me3/2和H2AK119ub1来控制基因表达。据报道，PcGs和/或由PcGs控制的基因的错误表达会导致几种类型的癌症，如乳腺癌、结肠癌、胰腺癌和肝癌。我们研究了与异位子宫内膜异位症患者相比，异位子宫内膜异位症组织中PcG蛋白如RING1B、BMI1和EZH2的失调是否与子宫内膜异位症组织中EMT所需的基因相关。我们量化了从12名因严重子宫内膜异位症接受腹腔镜检查的妇女中获得的配对异位和异位（子宫内膜异位症）组织样本中Polycomb抑制复合体1 （PRC1）基因（RING1B和BMI1）、emt相关基因（TWIST、SNAI1、SNAI2、ZEB1、CDH1、CDH2和VIM）的表达。我们的研究结果表明，与子宫内膜组织相比，子宫内膜异位症病变中PRC1蛋白- RING1B和BMI1基因表达较高，EMT相关基因表达较高。因此，我们的研究结果表明PRC1蛋白可能在子宫内膜异位症的发病机制中起作用。

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引用次数: 0

Origin, viability, and cell cycle status of seminal somatic cells for potential application in cattle cloning via nuclear transfer 牛核移植克隆中潜在应用的精体细胞的起源、活力和细胞周期状态。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-17 DOI: 10.1016/j.repbio.2025.101170

Higor da Silva Ferreira , Gabriella Borba de Oliveira , Eduardo de Oliveira Sanguinet , Ana Laura da Silva Feijó , César Augusto Pinzón-Osorio , Verônica Rafaela Benvenutti , Louise Fontoura Köhler , Marianna Bertolini , Fernando Almeida-Souza , Felipe de Jesus Moraes Junior , David Driemeier , Ana Lúcia Abreu-Silva , José Luiz Rigo Rodrigues , Marcelo Bertolini

Seminal somatic cells (SSCs) represent promising donor nuclei for cloning by somatic cell nuclear transfer (SCNT), particularly for genetic conservation. This study aimed to identify and characterize SSCs present in bovine semen, determine their possible tissue origins, and evaluate their suitability for cloning. SSCs were isolated using sucrose or mini-Percoll® density gradients and analyzed for morphology, viability, and cell cycle status, as well as their potential use in SCNT cloning. Cytological and histological assessments of the bovine genitourinary tract were performed side-by-side to identify the anatomical origins of each SSC type, and epithelial marker expression was examined by RT-qPCR in SSCs, urethral, and bladder tissues. The mini-Percoll® gradient efficiently separated SSCs from sperm cells, concentrating viable somatic cells in the 20 % fraction. Three SSC types were identified (polygonal, round, and elongated), with polygonal cells being the most abundant and highly viable (>90 %), although they failed to adhere or proliferate in culture. Flow cytometry revealed that most polygonal SSCs (89 %) were arrested at the G0/G1 phase, indicating cell-cycle compatibility for cloning, although with low membrane fusion capacity in SCNT assays. Cytological, histological, and molecular findings collectively indicate that polygonal SSCs most likely originate from the non-keratinized stratified epithelium of the urethral navicular fossa, whereas round and elongated SSCs may derive from the proximal urethra and the ampullary-vesicular-ductal complex, respectively. These results provide a comprehensive anatomical and molecular characterization of SSC subtypes in bovine semen, offering a foundation for improving SCNT cloning standardization and applications in genetic preservation.

在体细胞核移植（SCNT）技术中，生殖体细胞（ssc）是一种很有前途的供核，尤其在遗传保护方面。本研究旨在鉴定牛精液中存在的ssc，确定其可能的组织来源，并评估其克隆的适用性。使用蔗糖或mini-Percoll®密度梯度分离ssc，分析其形态、活力和细胞周期状态，以及它们在SCNT克隆中的潜在应用。同时对牛生殖泌尿道进行细胞学和组织学评估，以确定每种SSC类型的解剖学起源，并通过RT-qPCR检测SSC、尿道和膀胱组织中上皮标志物的表达。mini-Percoll®梯度有效地从精子细胞中分离ssc，浓缩20% %的活体细胞。鉴定出三种SSC类型（多边形、圆形和细长型），其中多边形细胞最丰富，存活率最高（bbb90 %），尽管它们在培养中不能粘附或增殖。流式细胞术显示，大多数多边形SSCs（89 %）停留在G0/G1期，表明细胞周期兼容性克隆，尽管在SCNT检测中膜融合能力较低。细胞学、组织学和分子检查结果共同表明，多边形SSCs最有可能起源于尿道舟窝的非角化层状上皮，而圆形和细长的SSCs可能分别来自尿道近端和壶腹-膀胱-导管复合体。这些结果为牛精液中SSC亚型的解剖和分子特征提供了全面的分析，为提高SCNT的克隆标准化和遗传保存应用奠定了基础。

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引用次数: 0

Mechanisms underlying Sorghum bicolor extract protection against monosodium glutamate-induced reproductive toxicity in female rats 高粱双色提取物对谷氨酸钠诱导的雌性大鼠生殖毒性的保护机制。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-12-03 DOI: 10.1016/j.repbio.2025.101168

Dumebi Anita Konwea , Jerome Ndudi Asiwe , Julian Enwerim Nwangwa , Blessing Zeinab Odili-Ovili , Saviour God’swealth Usin , Eze Kingsley Nwangwa

Monosodium glutamate (MSG) has been implicated in female reproductive toxicity through endocrine disruption, oxidative stress, inflammation, and apoptosis. This study investigated the protective role of Sorghum bicolor extract (SBE), a polyphenol-rich nutraceutical, against MSG-induced reproductive dysfunction in female Wistar rats, integrating in vivo outcomes with in silico docking analysis. Rats were exposed to MSG (4 g/kg) with or without SBE supplementation (100 mg/kg) orally for 28 days. Estrous cycle, sexual behaviors, hormonal profiles, oxidative and nitrosative stress markers, inflammatory mediators, and apoptotic regulators were assessed. Molecular docking evaluated the interactions of SBE phytochemicals with LHB and LHCGR. MSG disrupted the pituitary–gonadal axis, shortened estrous phases, suppressed reproductive behaviors, increased lipid peroxidation, activated NF-κB, and triggered mitochondrial apoptosis. SBE restored gonadotropins and steroid hormones, normalized estrous cycle, enhanced antioxidant defenses, suppressed inflammation, and prevented apoptosis. Docking analysis revealed strong ligand–protein interactions, supporting endocrine and cellular protection. Conclusively, SBE confers robust gonadoprotective effects against MSG toxicity through endocrine restoration, antioxidant, anti-inflammatory, and anti-apoptotic mechanisms validated in vivo and in silico.

味精（MSG）通过内分泌干扰、氧化应激、炎症和细胞凋亡与女性生殖毒性有关。本研究结合体内结果和硅对接分析，研究了富含多酚的营养制剂高粱双色提取物（SBE）对味精诱导的雌性Wistar大鼠生殖功能障碍的保护作用。大鼠口服味精（4 g/kg），并添加或不添加SBE(100 mg/kg)，持续28天。评估发情周期、性行为、激素谱、氧化和亚硝化应激标志物、炎症介质和凋亡调节因子。分子对接研究了SBE植物化学物质与LHB和LHCGR的相互作用。味精破坏垂体-性腺轴，缩短发情期，抑制生殖行为，增加脂质过氧化，激活NF-κB，引发线粒体凋亡。SBE恢复促性腺激素和类固醇激素，使发情周期正常化，增强抗氧化防御，抑制炎症，防止细胞凋亡。对接分析显示配体-蛋白相互作用强，支持内分泌和细胞保护。综上所述，SBE通过内分泌恢复、抗氧化、抗炎和抗凋亡机制对味精毒性具有强大的促性腺保护作用。

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引用次数: 0

Inhibitory role of activin A during luteinization of follicular and lutein granulosa cells from small antral and preovulatory human follicles 激活素A在卵泡黄体化和卵泡叶黄素颗粒细胞中的抑制作用。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-29 DOI: 10.1016/j.repbio.2025.101169

Lea Bejstrup Jensen , Laura Victoria Højer , Lisette Schönhage , Cristina Subiran Adrados , Jane Alrø Bøtkjær , Tanni Borgbo , Jesús Cadenas , Kirsten Tryde Macklon , Stine Ringholm , Anette Tønnes Pedersen , Stine Gry Kristensen

Luteinization is a vital process in female reproduction, where granulosa cells differentiate into progesterone-secreting luteal cells. While luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are established promoters of luteinization, the regulatory role of activin A remains incompletely understood. This study aimed to investigate whether activin A can prevent or reverse luteinization in granulosa cells from small antral and preovulatory follicles using an in vitro model. Granulosa cells were collected from women undergoing either IVF treatment or ovarian tissue cryopreservation. Cells were cultured for 48 h with FSH, hCG, or activin A. Hormone secretion (progesterone and estradiol) and content of steroidogenic markers (CYP19A1, STAR, 17βHSD1, 3βHSD2, FSHR, and LHCGR) were analyzed via ELISA, RT-qPCR, and Western blot.

Results

demonstrated that activin A suppressed luteinization markers and progesterone production while promoting estrogen synthesis and maintaining granulosa cell features. Conversely, hCG increased luteinization marker expression and progesterone secretion, consistent with its known role in luteal transformation. Notably, granulosa cells from small antral follicles showed a distinct, stage-specific response to activin A compared to those from preovulatory follicles, indicating a temporal regulation of luteinization competence.

These findings support a regulatory role for activin A in maintaining granulosa cell identity and delaying luteinization. Understanding the differential responsiveness of granulosa cells across follicular development stages may inform new therapeutic approaches for luteal phase deficiencies and fertility preservation strategies.

黄体生成是女性生殖过程中一个重要的过程，其中颗粒细胞分化为分泌黄体激素的黄体细胞。虽然黄体生成素（LH）和人绒毛膜促性腺激素（hCG）是已知的黄体生成素的促进因子，但活化素A的调节作用仍不完全清楚。本研究旨在通过体外模型研究激活素A是否能阻止或逆转小窦卵泡和排卵前卵泡颗粒细胞的黄体生成化。颗粒细胞从接受体外受精治疗或卵巢组织冷冻保存的妇女中收集。用FSH、hCG或activin a培养细胞48 h，通过ELISA、RT-qPCR和Western blot分析激素分泌（孕酮和雌二醇）和激素生成标志物（CYP19A1、STAR、17βHSD1、3βHSD2、FSHR和LHCGR）的含量。结果：激活素A抑制黄体生成素标记物和黄体酮的产生，同时促进雌激素合成，维持颗粒细胞特征。相反，hCG增加了黄体生成素标记物的表达和黄体酮的分泌，这与它在黄体转化中的已知作用一致。值得注意的是，与来自排卵前卵泡的颗粒细胞相比，来自小腔卵泡的颗粒细胞对激活素a表现出明显的阶段特异性反应，表明黄体生成素能力的时间调节。这些发现支持激活素a在维持颗粒细胞身份和延缓黄体生成中的调节作用。了解颗粒细胞在卵泡发育阶段的不同反应性可能为黄体期缺陷和生育保护策略提供新的治疗方法。

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引用次数: 0

Downregulation of intracellular ATP levels by PANX1 inhibits ovarian granulosa cell proliferation and mediates follicular dysplasia in elderly women PANX1下调细胞内ATP水平可抑制老年女性卵巢颗粒细胞增殖并介导卵泡发育不良。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-29 DOI: 10.1016/j.repbio.2025.101105

Zhiqin Zhang , Xuechen Sun , Xin Li , Peipei Liu , Liyun Cao , Shenggen Long , Jun Tan

This study examined pannexin 1 (PANX1) expression in ovarian granulosa cells of women with advanced maternal age and its role in cell proliferation and apoptosis, aiming to clarify mechanisms of age-related follicular dysplasia. Ninety women undergoing assisted reproductive technology were divided into reproductive-age (<35 years), advanced-age (35–41 years), and very advanced-age (≥42 years) groups. Clinical data and granulosa cell function were analyzed. PANX1 expression was detected in primary cells, while KGN cells were transfected with PANX1 plasmid or siRNA. Cell proliferation, apoptosis, and extracellular ATP levels were evaluated. With increasing age, oocyte yield, blastocyst formation, and pregnancy rates declined, granulosa cell proliferation decreased, apoptosis increased, and PANX1 expression was elevated. PANX1 overexpression inhibited proliferation and increased extracellular ATP, whereas knockdown enhanced proliferation without affecting apoptosis. PANX1 upregulation in aging granulosa cells mediates ATP efflux, depletes intracellular ATP, and suppresses proliferation, contributing to abnormal follicular development and reduced fertility.

本研究通过检测pannexin 1 （PANX1）在高龄产妇卵巢颗粒细胞中的表达及其在细胞增殖和凋亡中的作用，旨在阐明年龄相关性卵泡发育不良的机制。90名接受辅助生殖技术的妇女被分为育龄(

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引用次数: 0

Genotypic and metabolic impact of the SOD1 I>D polymorphism in polycystic ovary syndrome 多囊卵巢综合征SOD1 I>D多态性的基因型和代谢影响

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-26 DOI: 10.1016/j.repbio.2025.101166

Mehmet Can Nacar , Recai Aci̇ , Şengül Tural , Serbülent Yi̇ği̇t

This study aimed to investigate the association between the SOD1 gene I>D polymorphism, a key component of the antioxidant defense system, and polycystic ovary syndrome (PCOS), as well as to evaluate the potential effects of this genetic variation on genotypic distribution and metabolic parameters. A total of 100 PCOS patients and 135 healthy controls were included, and genomic DNA extracted from peripheral blood was analyzed for the SOD1 I>D polymorphism using conventional PCR, while clinical and biochemical parameters such as body mass index (BMI), insulin levels, HOMA-IR, FSH, LH, and other hormone levels were assessed. The ID genotype was found to be less frequent in the PCOS group compared to controls, with borderline statistical significance (p = 0.050; OR = 0.57), and was significantly less common in PCOS patients with a positive family history (p = 0.013), suggesting a potential protective effect, while correlations were observed between the ID genotype and markers of insulin resistance, though no significant differences were detected in allele frequencies. These findings indicate that the SOD1 gene I>D polymorphism may influence both susceptibility and metabolic characteristics of PCOS, supporting the role of oxidative stress in its pathogenesis and emphasizing the need for further functional and large-scale studies to confirm these associations.

本研究旨在探讨抗氧化防御系统关键成分SOD1基因I>；D多态性与多囊卵巢综合征（PCOS）的关系，并评估该基因变异对基因型分布和代谢参数的潜在影响。纳入100例PCOS患者和135例健康对照者，采用常规PCR方法提取外周血基因组DNA进行SOD1 I>；D多态性分析，同时评估临床生化指标如体重指数（BMI）、胰岛素水平、HOMA-IR、FSH、LH等激素水平。与对照组相比，PCOS组ID基因型较少出现，差异有统计学意义（p = 0.050;OR = 0.57），有阳性家族史的PCOS患者ID基因型较少出现（p = 0.013），提示有潜在的保护作用，而ID基因型与胰岛素抵抗标志物之间存在相关性，但等位基因频率无显著差异。这些发现表明SOD1基因I>；D多态性可能影响PCOS的易感性和代谢特征，支持氧化应激在其发病机制中的作用，并强调需要进一步的功能和大规模研究来证实这些关联。

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引用次数: 0

Mechanism of PP2A affecting ubiquitination pathway in spermatogenesis PP2A影响精子发生中泛素化途径的机制

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-25 DOI: 10.1016/j.repbio.2025.101167

Huamei Ju , Ziliang Geng , Binyan Chen , Yuwei Shang , Xia Chen , Danni Wang , Wenbin Wang , Huiting Sun , Yichao Shi , Jiajun Yu

The heterotrimeric complex serine-threonine protein phosphatase 2 A (PP2A) is integral to the regulation of essential cellular processes. It is particularly crucial in spermatogenesis, where it is indispensable for meiosis, mitosis, sperm capacitation, and apoptosis. Previous research has concentrated on the knockdown of the catalytic subunit of PP2A, PPP2CA, in germ cells using Ddx4-Cre, resulting in male mouse sterility, disrupted meiotic recombination, and meiotic arrest of spermatocytes. To further elucidate the role of PP2A in spermatogenesis, we performed transcriptomic and proteomic sequencing analyses on the testes of knockout and control mice. A nine-quadrant map was developed to depict the differential expression of genes and proteins. Our analyses identified 1732 differentially expressed genes, which exhibited a strong positive correlation with the trends in differential protein expression. Gene Ontology (GO) enrichment analysis indicated a significant downregulation of genes involved in spermatogenesis, sperm cell development, and sperm cell differentiation. Furthermore, KEGG enrichment analysis revealed a notable enrichment of differentially expressed genes within the ubiquitin-mediated proteolysis pathway. In knockout mouse testicular tissue, testicular expression of the ubiquitin-related gene, UBE2K, was markedly downregulated, which was associated with the accumulation of histone H3, upregulation of the methyltransferase SETDB1, and increased levels of H3K9me3. Similarly, knockdown of Ppp2ca in GC2 cells resulted in decreased UBE2K expression, histone H3 accumulation, SETDB1 upregulation, and elevated H3K9me3 levels, consistent with mirroring the phenotype observed in the knockout mice. Notably, the ubiquitin-related gene UBE2K was identified as a significant outlier in the nine-quadrant map, and real-time quantitative PCR confirmed that UBE2K transcript levels were significantly reduced in knockout mice compared to wild-type controls. These findings suggest that PP2A may regulate histon.

异三聚体复合体丝氨酸-苏氨酸蛋白磷酸酶2 A （PP2A）是必不可少的细胞过程的调节。它在精子发生中尤其重要，在那里它对减数分裂、有丝分裂、精子获能和细胞凋亡是必不可少的。先前的研究主要集中在使用Ddx4-Cre敲除生殖细胞中PP2A的催化亚基PPP2CA，导致雄性小鼠不育、减数分裂重组中断和精母细胞减数分裂停滞。为了进一步阐明PP2A在精子发生中的作用，我们对敲除小鼠和对照小鼠的睾丸进行了转录组学和蛋白质组学测序分析。一个九象限的图谱被用来描绘基因和蛋白质的差异表达。我们的分析确定了1732个差异表达基因，这些基因与差异蛋白表达趋势表现出很强的正相关。基因本体（GO）富集分析表明，参与精子发生、精子细胞发育和精子细胞分化的基因显著下调。此外，KEGG富集分析显示，在泛素介导的蛋白水解途径中，差异表达基因显著富集。在敲除小鼠睾丸组织中，睾丸中泛素相关基因UBE2K的表达明显下调，这与组蛋白H3的积累、甲基转移酶SETDB1的上调以及H3K9me3水平的升高有关。同样，在GC2细胞中敲低Ppp2ca导致UBE2K表达降低，组蛋白H3积累，SETDB1上调，H3K9me3水平升高，与敲除小鼠的表型一致。值得注意的是，泛素相关基因UBE2K在九象限图谱中被鉴定为显著异常值，实时定量PCR证实，与野生型对照相比，敲除小鼠的UBE2K转录水平显著降低。这些发现表明PP2A可能调节组蛋白。

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引用次数: 0

Effect of exogenous melatonin on the testes of Wistar rats undergoing early weaning 外源性褪黑素对早期断奶Wistar大鼠睾丸的影响

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-21 DOI: 10.1016/j.repbio.2025.101103

José Anderson da Silva Gomes , Renan Gabriel da Silva Ferreira , Jennyfer Martins de Carvalho , Maria Luísa Figueira de Oliveira , Rubem Carlos Araújo Guedes , Elba Verônica Matoso Maciel de Carvalho , Leucio Duarte Vieira Filho , Bruno Mendes Tenorio , Fernanda das Chagas Angelo Mendes Tenorio

The aim of the present study was to investigate the activity of exogenous melatonin as a potential agent in reversing testicular structural damage induced by early weaning. For this purpose, Wistar rats were used and divided into four experimental groups: control; early weaning; early weaning treated with melatonin in a daily dose 10 mg/kg of body weight; and early weaning treated with a vehicle composed of ethanol and saline solution. Except for the control group, all animals were weaned on the 16th day after birth. Body weight was measured weekly, and euthanasia was performed on day 51. The testes were weighed and collected for histopathological, morphometric, immunohistochemical, and oxidative stress analyses, while serum was collected for biochemical and hormonal analyses. At the end of the experiment, the early weaning group exhibited an increase in body mass, as well as structural alterations, including a reduction in the diameter and height of the seminiferous tubule epithelium, along with atrophy, vacuolization, and degeneration of germ cells. This group also showed lower production of pachytene spermatocytes, Sertoli cells, and Leydig cells, increased oxidative stress, decreased testosterone concentration, an adverse lipid profile, and reduced PCNA labeling. In contrast, the group treated with melatonin demonstrated improvement in these parameters when compared to early weaning group. These findings suggest that melatonin exerts a restorative role in testicular tissue.

本研究的目的是研究外源性褪黑激素作为逆转早期断奶引起的睾丸结构损伤的潜在药物的活性。为此，采用Wistar大鼠，将其分为4个实验组：对照组；早期断奶;早期断奶用褪黑素治疗，每日剂量为10 mg/kg体重；早期脱机用乙醇和生理盐水溶液组成的载体处理。除对照组外，其余动物均于出生后第16天断奶。每周测量体重，第51天实施安乐死。称重后收集睾丸进行组织病理学、形态计量学、免疫组织化学和氧化应激分析，同时收集血清进行生化和激素分析。在实验结束时，早期断奶组表现出体重增加，结构改变，包括精小管上皮直径和高度减少，生殖细胞萎缩，空泡化和变性。该组还显示粗线精母细胞、支持细胞和间质细胞的产生减少，氧化应激增加，睾酮浓度降低，不利的脂质谱和PCNA标记减少。相比之下，与早期断奶组相比，接受褪黑激素治疗的组在这些参数上表现出改善。这些发现表明，褪黑激素在睾丸组织中发挥了恢复作用。

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引用次数: 0

Prediction of euploidy and probability of obtaining target number of euploid embryos through preimplantation genetic testing for aneuploidy 通过着床前非整倍体基因检测预测整倍体及获得目标数量整倍体胚胎的概率

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-20 DOI: 10.1016/j.repbio.2025.101104

Kazuma Onishi , Daichi Inoue , Yuta Kida , Masae Kojima , Chiharu Ishida , Takahiro Suzuki , Shuhei Kamada , Noritaka Fukunaga , Yoshimasa Asada

We aimed to develop prediction models estimating (1) the probability of obtaining a target number of euploid embryos per individual and (2) the probability of euploidy at the embryo level using data from preimplantation genetic testing for aneuploidy (PGT-A). This retrospective observational study included 664 individuals who underwent PGT-A between March 2020 and October 2024, totaling 5539 biopsied embryos (23.2 % euploid). Among them, 153 had no euploid embryos and 511 had at least one. Four models were developed: Models 1-A, 1-B, and 1-C predicted the probability of obtaining ≥ 1, ≥ 2, and ≥ 3 euploid embryos, respectively. Model 2 predicted the probability of euploidy per embryo. Multivariate logistic regression was used for individual-level models (1-A to 1-C), and a generalized estimating equation was applied for Model 2. Predictors included maternal age at retrieval, number of biopsied embryos, and embryo morphology. Area under the receiver operating characteristic curve (AUC) was used to examine the discriminative ability of models. Internal validation was performed using 10-fold cross-validation. Decision curve analysis assessed clinical utility. Models 1-A, 1-B, and 1-C demonstrated good discrimination (AUCs: 0.85–0.87), while Model 2 showed fair discrimination (AUC: 0.77). Decision curve analysis supported the clinical utility of all models at wide range of thresholds probabilties. The individual-level prediction models demonstrated good discriminative ability, while the embryo-level model showed fair discriminative ability. All models demonstrated potential clinical utility in supporting embryo selection.

我们的目的是建立预测模型来估计(1)每个个体获得目标数量的整倍体胚胎的概率和(2)胚胎水平上整倍体的概率，利用着床前非整倍体基因检测（PGT-A）的数据。这项回顾性观察性研究包括664名在2020年3月至2024年10月期间接受PGT-A的个体，共5539个活检胚胎（23.2% %整倍体）。其中，153只没有整倍体胚胎，511只至少有一个整倍体胚胎。建立了四种模型：模型1- a、1- b和1- c分别预测获得≥ 1、≥ 2和≥ 3个整倍体胚胎的概率。模型2预测了每个胚胎整倍性的概率。个体水平模型（1-A至1-C）采用多元逻辑回归，模型2采用广义估计方程。预测因子包括母亲取卵时的年龄、活检胚胎的数量和胚胎形态。采用受试者工作特征曲线下面积（AUC）来检验模型的判别能力。采用10倍交叉验证进行内部验证。决策曲线分析评估临床效用。模型1-A、1-B和1-C具有良好的判别性（AUC: 0.85 ~ 0.87），而模型2具有一般的判别性（AUC: 0.77）。决策曲线分析支持所有模型在大范围阈值概率下的临床应用。个体水平预测模型具有较好的判别能力，胚胎水平预测模型具有一般的判别能力。所有模型在支持胚胎选择方面都显示出潜在的临床效用。

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引用次数: 0

The mouse model of induced sperm DNA damage caused by polystyrene microplastics exhibited distinct transcriptomic and proteomic features 聚苯乙烯微塑料诱导精子DNA损伤小鼠模型表现出明显的转录组学和蛋白质组学特征

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology

Pub Date : 2025-11-20 DOI: 10.1016/j.repbio.2025.101096

Chenming Zhang , Xiaofei Han , Yifei Wang , Ruimin Ma , Sicheng Ma , Wenbang Liu , Zhe Chang , Zixue Sun

Polystyrene microplastics (PS-MPs) are extensively utilized in plastic goods worldwide. The ingestion of PS-MPs has resulted in a high rate of DNA fragmentation index (DFI), which can potentially result in infertility and recurrent spontaneous abortion. This study established and characterized a mouse model of polystyrene microplastic (PS-MP)-induced sperm DNA damage (DnaD), and concurrently analyzed the associated transcriptomic and proteomic profiles. Over a period of 60 days, male mice assigned to the PS group were given PS-MPs at a dose of 1 mg/kg/d while the control group was administered an equivalent volume of normal saline. Sperm DNA Fragmentation Index (DFI) was then assessed using the Sperm Chromatin Structure Assay (SCSA).The testis was examined using RNA-seq and data-independent acquisition (DIA) to detect the patterns of mRNA and protein expression. The PS group exhibited an significant increase in the sperm DFI. Compared with the control group, 874 differentially expressed genes (DEGs) and 164 differentially expressed proteins (DEPs) were identified in the PS group. These included Agt, Gstt1, Fetub, Akr1c12, Eln, Gaa, Ppic and Ltbp2. The PI3K/Akt and metabolic pathways exhibited significant enrichment of these genes. After a 60-day period of intragastric injection, our findings indicated that the administration of PS-MPs at a 1 mg/kg/d dosage can lead to DnaD in the sperm of male mice. The metabolic and PI3K/Akt signaling pathways could be associated with the reproductive toxicity of PS-MPs.

Summary sentence

The intake of PS-MPs mainly reduces DFI in mice via the metabolic and PI3K/Akt signaling pathways.

聚苯乙烯微塑料（PS-MPs）在世界范围内广泛应用于塑料制品。摄入PS-MPs导致DNA片段化指数（DFI）高，这可能导致不孕和复发性自然流产。本研究建立了聚苯乙烯微塑料（PS-MP）诱导的小鼠精子DNA损伤模型，并对其进行了转录组学和蛋白质组学分析。在60天的时间里，PS组雄性小鼠以1 mg/kg/d的剂量给予PS- mps，对照组给予等量生理盐水。然后使用精子染色质结构测定法（SCSA）评估精子DNA碎片指数（DFI）。使用RNA-seq和数据独立采集（DIA）检测睾丸mRNA和蛋白质的表达模式。PS组精子DFI显著升高。与对照组相比，PS组共鉴定出874个差异表达基因（DEGs）和164个差异表达蛋白（DEPs）。其中包括Agt、Gstt1、Fetub、Akr1c12、Eln、Gaa、Ppic和Ltbp2。这些基因在PI3K/Akt和代谢通路中显著富集。经60天灌胃后，我们的研究结果表明，以1 mg/kg/d的剂量给药PS-MPs可导致雄性小鼠精子中的dna ad。代谢和PI3K/Akt信号通路可能与PS-MPs的生殖毒性有关。PS-MPs的摄入主要通过代谢和PI3K/Akt信号通路降低小鼠DFI。

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