Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1638458
Vyshali Veerareddy, umaraswamy Gandla
Development and validation of RP-HPLC method for the simultaneous determination of Glecaprevir & Pibrentasvir in pure form and their combined tablet dosage forms. The current separation was achieved on a Chromasil ODS Column (4.6 x 250mm, 5.0 μ) using the composition of the mobile phase of (0.1 M KH 2 PO 4 buffer: methanol) in the ratio of (60:40) at pH 4.5. The pump flow rate was adjusted after several one milliliter per minute and both the samples were separated at 230nm. The retention times are 1.7 min and 2.3 min, for Glecaprevir and Pibrentasvir respectively with linear ranges 20 µg/ml-60 µg/ml and 50 µg/ml-150 µg/ml. The method was statistically validated for linearity, accuracy, precision and selectivity as per ICH guidelines. The drugs were subjected to stress conditions of hydrolysis, oxidation, photolysis of the RP-HPLC method. Both medications were tested under various stress forced conditions, including alkali, acid, oxidative, photodegradation, & hydrolysis degradation. The accuracy limit is the percentage recovery should be in the range of 98.0% - 101.0%. The total recovery was found to be 99.69% and 99.39% for Glecaprevir and Pibrentasvir. The validation of developed method shows that the accuracy is well within the limit, which shows that the method is capable of showing good accuracy and reproducibility. The robustness limit for mobile phase variation and flow rate variation are well within the limit, which shows that the method is having good system suitability and precision under given set of conditions.
{"title":"A NOVEL STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PIBRENTASVIR AND GLECAPREVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS","authors":"Vyshali Veerareddy, umaraswamy Gandla","doi":"10.31788/rjc.2023.1638458","DOIUrl":"https://doi.org/10.31788/rjc.2023.1638458","url":null,"abstract":"Development and validation of RP-HPLC method for the simultaneous determination of Glecaprevir & Pibrentasvir in pure form and their combined tablet dosage forms. The current separation was achieved on a Chromasil ODS Column (4.6 x 250mm, 5.0 μ) using the composition of the mobile phase of (0.1 M KH 2 PO 4 buffer: methanol) in the ratio of (60:40) at pH 4.5. The pump flow rate was adjusted after several one milliliter per minute and both the samples were separated at 230nm. The retention times are 1.7 min and 2.3 min, for Glecaprevir and Pibrentasvir respectively with linear ranges 20 µg/ml-60 µg/ml and 50 µg/ml-150 µg/ml. The method was statistically validated for linearity, accuracy, precision and selectivity as per ICH guidelines. The drugs were subjected to stress conditions of hydrolysis, oxidation, photolysis of the RP-HPLC method. Both medications were tested under various stress forced conditions, including alkali, acid, oxidative, photodegradation, & hydrolysis degradation. The accuracy limit is the percentage recovery should be in the range of 98.0% - 101.0%. The total recovery was found to be 99.69% and 99.39% for Glecaprevir and Pibrentasvir. The validation of developed method shows that the accuracy is well within the limit, which shows that the method is capable of showing good accuracy and reproducibility. The robustness limit for mobile phase variation and flow rate variation are well within the limit, which shows that the method is having good system suitability and precision under given set of conditions.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136259734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1638264
None H. Hidayat, M.M. Musawwa, F.I. Fajarwati
Moringa seeds are a class of edible oils that have activities such as antioxidants, antiaging, emollients, hair care, and skin lightening. This study aims to identify the potential of chemical compounds from moringa seed oil as an antibacterial against Streptococcus pyogenes bacteria. Analysis by GC-MS showed that Moringa seed oil contains 40% oleic acid which is the compound with the highest peak. The antibacterial activity of Moringa seed oil was categorized as strong with clear zones of 11 mm and 15 mm respectively by Moringa seed oil which was extracted using soxhletation and MAE methods. Moreover, the characterization using GC-MS identified the presence of high oleic acid in Moringa seed oil with an area of 40%. The extraction results identified their functional groups by Fourier Transform Infrared (FTIR). Phytochemical test results showed that the oil from Moringa seeds was proven to contain alkaloids, flavonoids, and polyphenols. FTIR analysis showed several functional groups consisting of C=O, CH 3 , CH 2 groups, C-O esters, and O-H acids .
{"title":"BIOACTIVITY OF CHEMICAL COMPOUNDS FROM MORINGA SEEDS (Moringa oleifera) AND ANTIBACTERIAL ACTIVITY TEST AGAINST Streptococcus pyogenes BACTERIA","authors":"None H. Hidayat, M.M. Musawwa, F.I. Fajarwati","doi":"10.31788/rjc.2023.1638264","DOIUrl":"https://doi.org/10.31788/rjc.2023.1638264","url":null,"abstract":"Moringa seeds are a class of edible oils that have activities such as antioxidants, antiaging, emollients, hair care, and skin lightening. This study aims to identify the potential of chemical compounds from moringa seed oil as an antibacterial against Streptococcus pyogenes bacteria. Analysis by GC-MS showed that Moringa seed oil contains 40% oleic acid which is the compound with the highest peak. The antibacterial activity of Moringa seed oil was categorized as strong with clear zones of 11 mm and 15 mm respectively by Moringa seed oil which was extracted using soxhletation and MAE methods. Moreover, the characterization using GC-MS identified the presence of high oleic acid in Moringa seed oil with an area of 40%. The extraction results identified their functional groups by Fourier Transform Infrared (FTIR). Phytochemical test results showed that the oil from Moringa seeds was proven to contain alkaloids, flavonoids, and polyphenols. FTIR analysis showed several functional groups consisting of C=O, CH 3 , CH 2 groups, C-O esters, and O-H acids .","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136259740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2021.1618165
N. Rani, P. Pavani, Chebolu Naga Sesha Sai Pavan Kumar, Satwinder Singh Marok, S. Tulasi
Water pollution has recently become a serious global problem and hence researchers were focusing to develop innovative methods for the purification of water and then getting access to purified water by reducing pollution. As a result, the current study concentrated on synthesizing cobalt-doped zinc oxide nanoparticles by accumulating cobalt ions on Pistia (Pistiastratiotes L.) plant tissue and combining them with zinc acetate precursor. Further, the synthesized particles were analyzed for their efficiency in photo-desulphurization of organo sulphur compounds such as Dibenzothiophene and 2,5-dimethyl thiophene. Various characterization studies proved that the nanoparticles were oval in shape and had particle sizes of 8-34 nm and the particles were distributed with less aggregation. The particles have 47 % of zinc element and 21% cobalt with hexagonal (wurtzite) crystalline structure. The synthesized Co-doped ZnO nanoparticles were utilized for the desulfurization of Sulphur containing pollutants such as dibenzothiophene and 2,5-dimethyl thiophene. The results proved that rapid desulfurization was observed during the initial time of the study. The % desulfurization of 14.36±0.136 and 19.63±0.201% was observed within 5 min of the study for dibenzothiophene and 2,5-dimethyl thiophene. Whereas the high % desulfurization was noticed within less time of 25 min wherein the % desulfurization was observed to be 95.61±0.279 and 93.66±0.137 for dibenzothiophene and 2,5-dimethyl thiophene respectively. The removal of harmful heavy metals utilizing the Pistia plant and recycling in the wastewater treatment process may therefore be inferred as being highly valued. Additionally, the Co-doped ZnO photocatalysts might improve photocatalytic activity owing to the catalysts' porosity and the hole recombination and electron suppression.
{"title":"GREEN SYNTHESIS AND CHARACTERIZATION OF CODOPED ZnO NANOPARTICLES VIA THE ACCUMULATION OF COBALT ION ONTO PISTIA (Pistia stratiotes L.,) PLANT TISSUE AND ITS PHOTOCATALYTIC ACTIVITY TOWARD ORGANOSULFUR POLLUTANTS","authors":"N. Rani, P. Pavani, Chebolu Naga Sesha Sai Pavan Kumar, Satwinder Singh Marok, S. Tulasi","doi":"10.31788/rjc.2021.1618165","DOIUrl":"https://doi.org/10.31788/rjc.2021.1618165","url":null,"abstract":"Water pollution has recently become a serious global problem and hence researchers were focusing to develop innovative methods for the purification of water and then getting access to purified water by reducing pollution. As a result, the current study concentrated on synthesizing cobalt-doped zinc oxide nanoparticles by accumulating cobalt ions on Pistia (Pistiastratiotes L.) plant tissue and combining them with zinc acetate precursor. Further, the synthesized particles were analyzed for their efficiency in photo-desulphurization of organo sulphur compounds such as Dibenzothiophene and 2,5-dimethyl thiophene. Various characterization studies proved that the nanoparticles were oval in shape and had particle sizes of 8-34 nm and the particles were distributed with less aggregation. The particles have 47 % of zinc element and 21% cobalt with hexagonal (wurtzite) crystalline structure. The synthesized Co-doped ZnO nanoparticles were utilized for the desulfurization of Sulphur containing pollutants such as dibenzothiophene and 2,5-dimethyl thiophene. The results proved that rapid desulfurization was observed during the initial time of the study. The % desulfurization of 14.36±0.136 and 19.63±0.201% was observed within 5 min of the study for dibenzothiophene and 2,5-dimethyl thiophene. Whereas the high % desulfurization was noticed within less time of 25 min wherein the % desulfurization was observed to be 95.61±0.279 and 93.66±0.137 for dibenzothiophene and 2,5-dimethyl thiophene respectively. The removal of harmful heavy metals utilizing the Pistia plant and recycling in the wastewater treatment process may therefore be inferred as being highly valued. Additionally, the Co-doped ZnO photocatalysts might improve photocatalytic activity owing to the catalysts' porosity and the hole recombination and electron suppression.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69405206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1616916
A. Gupta, P. Singour, S. K. Jain
This work reported a reverse phase-high performance liquid chromatography method for quantitative estimation of Azelastine Hydrochloride, Fluticasone Propionate, and Phenylethyl Alcohol in Nasal Spray formulation. The assay involved an isocratic elution of these components by using Intersil Octadecylsilane (C18) column (25 cm x 4.6 mm x 5 μm) using mobile phase composition of Buffer: Solvent Mixture (40:60 %v/v) and pH adjusted to 6.5 with dilute orthophosphoric acid. The flow rate was 1.4 mLmin-1 and the analytes are monitored at 254 nm. Separation was completed within 20 minutes. Calibration curves were linear with correlation coefficient more than 0.99 over a concentration range of 50%, 75%, 100%, 125%, and 150% for Azelastine Hydrochloride, Fluticasone Propionate and Phenylethyl Alcohol. The method was proven to be accurate between 50 to 150% with 98 to 102 % recovery of the actives from a spiked placebo. The method was shown to be precise yielding acceptable results for the system reproducibility and method repeatability. All the validation parameters were within the acceptance range according to International Conference on Harmonization norms. The developed method is simple and rapid which could be applied for routine estimation of the formulation.
本工作报道了反相高效液相色谱法定量测定鼻喷雾剂制剂中盐酸氮杂松碱、丙酸氟替卡松和苯乙醇的含量。实验采用Intersil十八烷基硅烷(C18)色谱柱(25 cm × 4.6 mm × 5 μm)，流动相为缓冲液:溶剂混合物(40:60 %v/v)，用稀正磷酸调节pH至6.5，等密度洗脱这些成分。流速为1.4 mLmin-1，检测波长为254 nm。分离在20分钟内完成。盐酸氮扎elastine、丙酸氟替卡松和苯乙醇在50%、75%、100%、125%和150%的浓度范围内均呈线性，相关系数大于0.99。该方法的准确度在50 ~ 150%之间，加标安慰剂的活性回收率为98 ~ 102%。结果表明，该方法精度高，系统重现性和方法重现性均可接受。所有验证参数均在国际协调会议规范的可接受范围内。该方法简便、快速，可用于配方的常规估算。
{"title":"SIMULTANEOUS REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC) ESTIMATION OF AZELASTINE HYDROCHLORIDE, FLUTICASONE PROPIONATE, AND PHENYL ETHYL ALCOHOL IN DYMISTA (MEDA) NASAL SPRAY","authors":"A. Gupta, P. Singour, S. K. Jain","doi":"10.31788/rjc.2023.1616916","DOIUrl":"https://doi.org/10.31788/rjc.2023.1616916","url":null,"abstract":"This work reported a reverse phase-high performance liquid chromatography method for quantitative estimation of Azelastine Hydrochloride, Fluticasone Propionate, and Phenylethyl Alcohol in Nasal Spray formulation. The assay involved an isocratic elution of these components by using Intersil Octadecylsilane (C18) column (25 cm x 4.6 mm x 5 μm) using mobile phase composition of Buffer: Solvent Mixture (40:60 %v/v) and pH adjusted to 6.5 with dilute orthophosphoric acid. The flow rate was 1.4 mLmin-1 and the analytes are monitored at 254 nm. Separation was completed within 20 minutes. Calibration curves were linear with correlation coefficient more than 0.99 over a concentration range of 50%, 75%, 100%, 125%, and 150% for Azelastine Hydrochloride, Fluticasone Propionate and Phenylethyl Alcohol. The method was proven to be accurate between 50 to 150% with 98 to 102 % recovery of the actives from a spiked placebo. The method was shown to be precise yielding acceptable results for the system reproducibility and method repeatability. All the validation parameters were within the acceptance range according to International Conference on Harmonization norms. The developed method is simple and rapid which could be applied for routine estimation of the formulation.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69409717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1616951
Ruslan, J. Hardi, M. Mirzan, Muliana, M. Ganti
Meti shell contains calcium carbonate which can be calcined into CaO and used as a heterogeneous catalyst in biodiesel synthesis. This study evaluated the ability of CaO catalyst from Meti shell with concentrations of 1, 2, 3, 4, and 5% for the synthesis of biodiesel from Moringa seed oil at various transesterification times (1, 2, 3, and 4 hours). The presence of CaO in the Meti shell was indicated by diffraction at 2θ angles of 32.47o , 37.74o , 54.22o , 64.65o , and 67.67oC. Utilizing CaO 3% and a transesterification reaction time of 3 hours resulted in an Rf value corresponding to the standard biodiesel Rf ranges, i.e. 0.69 and 0.66, respectively. The synthesized biodiesel has the highest methyl oleate content of 59.16% with characteristics that have met Indonesian National Standard (SNI 04-7182-2006), including water content of 0.004%, an acid number of 0.06 mg KOH/g, saponification number 273.6 mgKOH/g, iodine number 78.678 g iodine/100 g, the cloud point is 10oC, the pour point is 21oC, however, the cetane number is still below the minimum number of 51.
{"title":"APPLICATION OF HETEROGENEOUS CATALYST OF Meti SHELLS (Batissa violecea L. von Lamark 1818) IN THE PRODUCTION OF BIODIESEL BASED ON MORINGA SEED OIL","authors":"Ruslan, J. Hardi, M. Mirzan, Muliana, M. Ganti","doi":"10.31788/rjc.2023.1616951","DOIUrl":"https://doi.org/10.31788/rjc.2023.1616951","url":null,"abstract":"Meti shell contains calcium carbonate which can be calcined into CaO and used as a heterogeneous catalyst in biodiesel synthesis. This study evaluated the ability of CaO catalyst from Meti shell with concentrations of 1, 2, 3, 4, and 5% for the synthesis of biodiesel from Moringa seed oil at various transesterification times (1, 2, 3, and 4 hours). The presence of CaO in the Meti shell was indicated by diffraction at 2θ angles of 32.47o , 37.74o , 54.22o , 64.65o , and 67.67oC. Utilizing CaO 3% and a transesterification reaction time of 3 hours resulted in an Rf value corresponding to the standard biodiesel Rf ranges, i.e. 0.69 and 0.66, respectively. The synthesized biodiesel has the highest methyl oleate content of 59.16% with characteristics that have met Indonesian National Standard (SNI 04-7182-2006), including water content of 0.004%, an acid number of 0.06 mg KOH/g, saponification number 273.6 mgKOH/g, iodine number 78.678 g iodine/100 g, the cloud point is 10oC, the pour point is 21oC, however, the cetane number is still below the minimum number of 51.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69409725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1617074
M. F. Lubis, P. Hasibuan, V. E. Kaban, R. Astyka
The use of chemotherapy such as cisplatin in treating cancer alone can cause many problems, including side effects and resistance. Therefore, currently, efforts are being made to combine cisplatin with natural ingredients such as ethanol extract from duku leaves. Then extracted using ethanol as a solvent. The secondary metabolites of the extract were identified using LC-MS/MS with mobile phase A (0.1 percent formic acid in water) and phase B (0.1 percent formic acid in acetonitrile). The extract and cisplatin were determined by their IC50 values as the basis for carrying out the combination test using the MTT method. All data obtained were processed with Microsoft Excel and analyzed with SPSS 23. The ethanol extract of duku leaves contains about 15 compounds that cannot be clearly identified because they are not listed in the LC-MS/MS library used. Interestingly, there were 5 compounds that gave a sufficiently large absorption each at a retention time of 1.08; 5.18; 9.45; 9.57; 10.70; and 10.82. Cisplatin has stronger cytotoxic properties than the extract with IC50 values of 3.02±0.05 µg/mL and 28.61±0.13 µg/mL, respectively. The combination of these two substances is synergistic with a combination index <1. the combination of extract and cisplatin at doses of 1.75:0.75 and 1.75:0.375 had very strong synergy with a combination index of 0.01 (p>0.05). However, the mechanism of action of this combination is not yet known clearly, so further research is needed on it.
{"title":"PHYTOCHEMICALS ANALYSIS AND CYTOTOXIC ACTIVITY OF Lansium domesticum Corr EXTRACT-CISPLATIN COMBINATION AGAINST PANC-1 CELL LINE","authors":"M. F. Lubis, P. Hasibuan, V. E. Kaban, R. Astyka","doi":"10.31788/rjc.2023.1617074","DOIUrl":"https://doi.org/10.31788/rjc.2023.1617074","url":null,"abstract":"The use of chemotherapy such as cisplatin in treating cancer alone can cause many problems, including side effects and resistance. Therefore, currently, efforts are being made to combine cisplatin with natural ingredients such as ethanol extract from duku leaves. Then extracted using ethanol as a solvent. The secondary metabolites of the extract were identified using LC-MS/MS with mobile phase A (0.1 percent formic acid in water) and phase B (0.1 percent formic acid in acetonitrile). The extract and cisplatin were determined by their IC50 values as the basis for carrying out the combination test using the MTT method. All data obtained were processed with Microsoft Excel and analyzed with SPSS 23. The ethanol extract of duku leaves contains about 15 compounds that cannot be clearly identified because they are not listed in the LC-MS/MS library used. Interestingly, there were 5 compounds that gave a sufficiently large absorption each at a retention time of 1.08; 5.18; 9.45; 9.57; 10.70; and 10.82. Cisplatin has stronger cytotoxic properties than the extract with IC50 values of 3.02±0.05 µg/mL and 28.61±0.13 µg/mL, respectively. The combination of these two substances is synergistic with a combination index <1. the combination of extract and cisplatin at doses of 1.75:0.75 and 1.75:0.375 had very strong synergy with a combination index of 0.01 (p>0.05). However, the mechanism of action of this combination is not yet known clearly, so further research is needed on it.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69409795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1618129
V. Ragunathan, Meenambiga Setti Sudharsan, Roshini S.R., Gomathy Sundar, Chithra K.
This research scintillates the synthesis of silver nanoparticles from chitosan (C-AgNps) from squid pens as a reducing and stabilizing agent and assesses its antioxidant, antibacterial, and anticancer potentials. The nanoparticles were 240 to 290 nm with excellent stability of +98.2 mV. The C-AgNps possessed an excellent 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity with an IC50 of 6.65 µg/mL. The C-AgNps showed exceptional antibacterial properties against S. aureus, B. subtilis, P. aeruginosa, and E. coli using a good diffusion assay. The C-AgNps also showed outstanding anticancer properties against A375 human skin malignant melanoma. Thus, the C-AgNps could be used as antimicrobial and anticancer agents for novel therapeutics after lead optimization and in-vivo clinical trials.
{"title":"SQUID PENS MEDIATED SILVER NANOPARTICLES SYNTHESIS, ITS CHARACTERISATION, AND BIOLOGICAL ACTIVITIES","authors":"V. Ragunathan, Meenambiga Setti Sudharsan, Roshini S.R., Gomathy Sundar, Chithra K.","doi":"10.31788/rjc.2023.1618129","DOIUrl":"https://doi.org/10.31788/rjc.2023.1618129","url":null,"abstract":"This research scintillates the synthesis of silver nanoparticles from chitosan (C-AgNps) from squid pens as a reducing and stabilizing agent and assesses its antioxidant, antibacterial, and anticancer potentials. The nanoparticles were 240 to 290 nm with excellent stability of +98.2 mV. The C-AgNps possessed an excellent 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity with an IC50 of 6.65 µg/mL. The C-AgNps showed exceptional antibacterial properties against S. aureus, B. subtilis, P. aeruginosa, and E. coli using a good diffusion assay. The C-AgNps also showed outstanding anticancer properties against A375 human skin malignant melanoma. Thus, the C-AgNps could be used as antimicrobial and anticancer agents for novel therapeutics after lead optimization and in-vivo clinical trials.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69410573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1626922
R. Futeri, S. Samah, G. R. Putri
Carboxymethyl Cellulose, one of the additives commonly used in the industrial sector, is carboxymethyl cellulose. The state of the art of this research is because no research makes a reactor to manufacture carboxymethyl cellulose. This study tested the quality of making carboxymethyl cellulose using a semi-batch reactor using samples of potential organic cellulose waste materials: durian seeds, taro rods, and bagasse. This sample has been used to make carboxymethyl cellulose in previous studies using a laboratory scale. Then compared, the manufacture of carboxymethyl cellulose using a reactor laboratory scale. From the result obtained, carboxymethyl cellulose from durian seeds, taro rod, and bagasse made on a laboratory scale and using a reactor has become carboxymethyl cellulose in quality number 1 and 2, which are commonly called carboxymethyl cellulose industrial grade and food chemical codex quality which met the minimum quality requirements by SNI 8762; 2019. Carboxymethyl cellulose from durian seeds, taro rod, and bagasse obtained from research that has been tested with FTIR, the spectra of the best results of carboxymethyl cellulose from durian seeds, taro rod, and bagasse can be seen in the picture there are similar peaks that appear indicates carboxymethyl cellulose durian seeds, carboxymethyl cellulose taro rod, and carboxymethyl cellulose bagasse produced in this study have the same chemical molecule as industrial grade and food chemical codex.1.2
{"title":"QUALITY TEST OF CMC (CARBOXYMETHYL CELLULOSE) USING SEMI-BATCH REAKTOR","authors":"R. Futeri, S. Samah, G. R. Putri","doi":"10.31788/rjc.2023.1626922","DOIUrl":"https://doi.org/10.31788/rjc.2023.1626922","url":null,"abstract":"Carboxymethyl Cellulose, one of the additives commonly used in the industrial sector, is carboxymethyl cellulose. The state of the art of this research is because no research makes a reactor to manufacture carboxymethyl cellulose. This study tested the quality of making carboxymethyl cellulose using a semi-batch reactor using samples of potential organic cellulose waste materials: durian seeds, taro rods, and bagasse. This sample has been used to make carboxymethyl cellulose in previous studies using a laboratory scale. Then compared, the manufacture of carboxymethyl cellulose using a reactor laboratory scale. From the result obtained, carboxymethyl cellulose from durian seeds, taro rod, and bagasse made on a laboratory scale and using a reactor has become carboxymethyl cellulose in quality number 1 and 2, which are commonly called carboxymethyl cellulose industrial grade and food chemical codex quality which met the minimum quality requirements by SNI 8762; 2019. Carboxymethyl cellulose from durian seeds, taro rod, and bagasse obtained from research that has been tested with FTIR, the spectra of the best results of carboxymethyl cellulose from durian seeds, taro rod, and bagasse can be seen in the picture there are similar peaks that appear indicates carboxymethyl cellulose durian seeds, carboxymethyl cellulose taro rod, and carboxymethyl cellulose bagasse produced in this study have the same chemical molecule as industrial grade and food chemical codex.1.2","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69410795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1628147a
K. Laavanya, B. Manju, D. A. Shirmila, M. Priya, J. Hemalatha, G. Usha
The recent global COVID-19 pandemic has drawn awareness among the people towards Kabasura Kudineer (KSK) and its healthcare benefits in Tamil Nadu, India. It has been traditionally used for effective management of respiratory ailments such as flu and colds. Nine Phytochemicals were identified through the Gas ChromatographyMass Spectrometry (GCMS) study, from the KSK extract with ethanol. Fourier transforms infrared (FT-IR), FTRAMAN, and UV studies were carried out for spectral investigations on the same KSK extract. The in vitro evaluation reveals the antimicrobial, cytotoxicity, and anticancer activity against human carcinoma (HT29) cells. One of the compounds was utilized for AutoDocking to check the anticancer efficacy of the KSK extract.
{"title":"GC-MS AND OPTICAL CHARACTERIZATION, BIOLOGICAL PROPERTY STUDIES OF ETHANOLIC EXTRACT OF KABASURAKUDINEER AND MOLECULAR DOCKING INVESTIGATION OF (E)-3-PHENYL-1-(2, 2, 4- TRIMETHYLQUINOLIN-1-YL) PROP-2-EN-1-ONE","authors":"K. Laavanya, B. Manju, D. A. Shirmila, M. Priya, J. Hemalatha, G. Usha","doi":"10.31788/rjc.2023.1628147a","DOIUrl":"https://doi.org/10.31788/rjc.2023.1628147a","url":null,"abstract":"The recent global COVID-19 pandemic has drawn awareness among the people towards Kabasura Kudineer (KSK) and its healthcare benefits in Tamil Nadu, India. It has been traditionally used for effective management of respiratory ailments such as flu and colds. Nine Phytochemicals were identified through the Gas ChromatographyMass Spectrometry (GCMS) study, from the KSK extract with ethanol. Fourier transforms infrared (FT-IR), FTRAMAN, and UV studies were carried out for spectral investigations on the same KSK extract. The in vitro evaluation reveals the antimicrobial, cytotoxicity, and anticancer activity against human carcinoma (HT29) cells. One of the compounds was utilized for AutoDocking to check the anticancer efficacy of the KSK extract.","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69411025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.31788/rjc.2023.1628164
D. Purwaningsih, H. Sutrisno, K. Sugiyarto, C. Kusumawardani, N. A. Kusumaningrum
The aim of this study was to synthesize LiFe1-xNixPO4/C with x = 0; 0.05; 0.07; 0.09 using the reflux method assisted by microwave irradiation. The goal was to increase the value of the electronic conductivity and reaction rate of Li+ compounds. The results showed that the method successfully synthesized LiFePO4/C and LiFe1-xNixPO4/C with an orthorhombic crystal structure in the Pnma space group. The optimum reflux-microwave irradiation time was 10 minutes, and increasing moles of Ni doping lead to a decrease in the lattice parameters cell volume. The crystalline size ranged from 21.42–22.62 nm, and the surface of the compound particles was irregular. The compound LiFe0.93Ni0.07PO4/C had the highest conductivity (5.45 × 10-7 S/cm).
{"title":"SYNTHESIS OF LiFe1-xNixPO4/C USING THE REFLUX WITH MICROWAVE IRRADIATION METHOD","authors":"D. Purwaningsih, H. Sutrisno, K. Sugiyarto, C. Kusumawardani, N. A. Kusumaningrum","doi":"10.31788/rjc.2023.1628164","DOIUrl":"https://doi.org/10.31788/rjc.2023.1628164","url":null,"abstract":"The aim of this study was to synthesize LiFe1-xNixPO4/C with x = 0; 0.05; 0.07; 0.09 using the reflux method assisted by microwave irradiation. The goal was to increase the value of the electronic conductivity and reaction rate of Li+ compounds. The results showed that the method successfully synthesized LiFePO4/C and LiFe1-xNixPO4/C with an orthorhombic crystal structure in the Pnma space group. The optimum reflux-microwave irradiation time was 10 minutes, and increasing moles of Ni doping lead to a decrease in the lattice parameters cell volume. The crystalline size ranged from 21.42–22.62 nm, and the surface of the compound particles was irregular. The compound LiFe0.93Ni0.07PO4/C had the highest conductivity (5.45 × 10-7 S/cm).","PeriodicalId":21063,"journal":{"name":"Rasayan Journal of Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69411105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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