Endotoxin Lipopolysaccharide (LPS) induces an innate immune response and activates sickness behavior. During neuroinflammation, the activated microglial cells change its morphology and release various chemicals that could be harmful such as nitric oxide (NO), reactive oxygen species (ROS) and enzymes for proteolysis. Through the buildup of free radicals and ROS, these neuroinflammations can also result in oxidative stress. This ROS reacts with cellular macromolecules, triggering a cascade that results in membrane porosity caused by membrane lipid peroxidation (LPO), which is measured by the quantity of malondialdehyde (MDA). On LPS challenge, the complex behaviour that decreased common activities and explorations, is noticed and with rising anxiety, cognitive impairment and decreased social activities. It also decreased closed arm time, although closed arm entries were higher which may be related to the increased locomotion. It raised open arm time and open arm entries, suggesting of reduced anxiety and also increased exploratory behaviour. AChE and nNOS were increased in the forebrain part. Reduction in brainstem and MDA activity decreased on both forebrain and brain stem. MDA in the liver increased by LPS challenge indicating upregulated hepatic tissue damage and oxidative stress. As a result of LPS-induced lipid peroxidation and oxidative stress, brain Glutathione (GSH), a substrate for cytosolic Glutathione peroxidase, increased in the forebrain and decreased in the brainstem. Decreased scavenging of ROS was indicated by the LPS-induced decrease in liver GSH levels.
{"title":"Endotoxin lipopolysaccharide (LPS)-induced peripheral inflammation and its impact on brain and liver function","authors":"Hariprasad Shetty, Reshma A. Shetty","doi":"10.25303/1809rjbt037043","DOIUrl":"https://doi.org/10.25303/1809rjbt037043","url":null,"abstract":"Endotoxin Lipopolysaccharide (LPS) induces an innate immune response and activates sickness behavior. During neuroinflammation, the activated microglial cells change its morphology and release various chemicals that could be harmful such as nitric oxide (NO), reactive oxygen species (ROS) and enzymes for proteolysis. Through the buildup of free radicals and ROS, these neuroinflammations can also result in oxidative stress. This ROS reacts with cellular macromolecules, triggering a cascade that results in membrane porosity caused by membrane lipid peroxidation (LPO), which is measured by the quantity of malondialdehyde (MDA). On LPS challenge, the complex behaviour that decreased common activities and explorations, is noticed and with rising anxiety, cognitive impairment and decreased social activities. It also decreased closed arm time, although closed arm entries were higher which may be related to the increased locomotion. It raised open arm time and open arm entries, suggesting of reduced anxiety and also increased exploratory behaviour. AChE and nNOS were increased in the forebrain part. Reduction in brainstem and MDA activity decreased on both forebrain and brain stem. MDA in the liver increased by LPS challenge indicating upregulated hepatic tissue damage and oxidative stress. As a result of LPS-induced lipid peroxidation and oxidative stress, brain Glutathione (GSH), a substrate for cytosolic Glutathione peroxidase, increased in the forebrain and decreased in the brainstem. Decreased scavenging of ROS was indicated by the LPS-induced decrease in liver GSH levels.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rheum is an important medicinal plant belonging to family Polygonaceae and is found to have anti-microbial, anti-purgative and anti-cancer properties. The objective of the present study is to standardize an effective and low-cost method for the extraction of DNA from the young juvenile leaves of Rheum plant obtained from the Uttarakhand region. The protocol involved the extraction of DNA using a modified CTAB method without the use of any liquid nitrogen. The concentration of NaCl used in extraction buffer was slightly higher (1.4M) to remove polysaccharides and varying ratios of Beta-mercaptoethanol used to remove the secondary metabolites and keep them away from interfering the extraction process. The absorbance ratio of the extracted DNA at A260/280 nm was within the permissible range of 1.7-2.0, indicating the purity of DNA and showing that the DNA is contamination free. Its concentration was approximately 1485 ng/μl and it was found suitable for molecular-based study.
{"title":"An effective and low-cost method for DNA extraction from Rheum species (A medicinal herb) without using liquid nitrogen for RAPD/ISSR Study","authors":"Anjali Uniyal, Akhilesh Kumar, Vijay Kumar, Sanjay Gupta, Vivek Kumar","doi":"10.25303/1809rjbt1700174","DOIUrl":"https://doi.org/10.25303/1809rjbt1700174","url":null,"abstract":"Rheum is an important medicinal plant belonging to family Polygonaceae and is found to have anti-microbial, anti-purgative and anti-cancer properties. The objective of the present study is to standardize an effective and low-cost method for the extraction of DNA from the young juvenile leaves of Rheum plant obtained from the Uttarakhand region. The protocol involved the extraction of DNA using a modified CTAB method without the use of any liquid nitrogen. The concentration of NaCl used in extraction buffer was slightly higher (1.4M) to remove polysaccharides and varying ratios of Beta-mercaptoethanol used to remove the secondary metabolites and keep them away from interfering the extraction process. The absorbance ratio of the extracted DNA at A260/280 nm was within the permissible range of 1.7-2.0, indicating the purity of DNA and showing that the DNA is contamination free. Its concentration was approximately 1485 ng/μl and it was found suitable for molecular-based study.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study aimed to bio-synthesize cerium oxide nanoparticles from the root extract of Withania somnifera (Ashwagandha) and to evaluate their antioxidant potential. In Indian Ayurvedic medicine, Withania somnifera has been used as a memory booster and to treat cancer and neurological disorders. Several characterization techniques such as XRD, FTIR, UV-Vis, Raman spectroscopy, SEM-EDX and HR-TEM, were used to confirm the formation of CeO2NPs. The characterization results indicate that synthesized NPs were spherical with crystalline nature and size <10nm. An OH radical scavenging assay was done to monitor the antioxidant property of synthesized CeO2NPs. The maximum absorbance at 532 nm was measured by discoloration from light pinkish to reddish-orange. CeO2NPs exhibited significant OH radical scavenging activity than vitamin C. Therefore, our results suggest that biogenic CeO2NPs have shown good antioxidant potential and may be used to treat oxidative stress-related diseases.
{"title":"Biogenic synthesis of Cerium oxide nanoparticles and their antioxidant activity","authors":"Gopinath Mamatha Madhugiri, Sultana Sumreen, Manohar Punekar Shital, M.B. Nivedika, Tekupalli Ravikiran","doi":"10.25303/1809rjbt023028","DOIUrl":"https://doi.org/10.25303/1809rjbt023028","url":null,"abstract":"The present study aimed to bio-synthesize cerium oxide nanoparticles from the root extract of Withania somnifera (Ashwagandha) and to evaluate their antioxidant potential. In Indian Ayurvedic medicine, Withania somnifera has been used as a memory booster and to treat cancer and neurological disorders. Several characterization techniques such as XRD, FTIR, UV-Vis, Raman spectroscopy, SEM-EDX and HR-TEM, were used to confirm the formation of CeO2NPs. The characterization results indicate that synthesized NPs were spherical with crystalline nature and size <10nm. An OH radical scavenging assay was done to monitor the antioxidant property of synthesized CeO2NPs. The maximum absorbance at 532 nm was measured by discoloration from light pinkish to reddish-orange. CeO2NPs exhibited significant OH radical scavenging activity than vitamin C. Therefore, our results suggest that biogenic CeO2NPs have shown good antioxidant potential and may be used to treat oxidative stress-related diseases.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.25303/1809rjbt1910197
Pooja Singh Gaur, Surya Kant, Rachna Chaturvedi
Genetic risk assessment of tuberculosis development and genotypic variation influence the gene and protein expression of leukotriene A4 hydrolase (LTA4H) at various sites of tuberculosis. The relationship between the genotype and phenotype of LATH4A was investigated in relation to the genetic risk assessment of extrapulmonary tuberculosis (EPTB). This study included 137 patients with EPTB and age-sex-matched 137 normal controls of the same ethnicity. LTA4H genotyping was performed using restriction fragment polymorphisms (RFLP) and protein level was analyzed using enzyme-linked immunosorbent assay (ELISA). The LTAH4 (rs.197833A/G) AG (codominant) and GG (recessive) genotypes showed 1.86 and 1.88-fold higher risks (p=0.0004 and p<0.0001 respectively) of developing EPTB. LTA4H (rs.2660898 T/G) TG (codominant) and GG (recessive) genotypes significantly increased the risk (OR:1.69, p=0.0001 and OR;1.77 p=0.008 respectively) of EPTB. Similarly, LTA4H (rs. 2540474) AG (codominant) and GG (recessive) genotypes (OR; 1.58 95% CI:1.22- 2.04, p<0.0001; OR, 1.86; 95%CI 1.37-2.52, p<0.0001) increased the risk of developing ETTB. The LTB4 protein levels were significantly higher in EPTB cases (mean ± SD; 3096.10±1287.6 pg/mL) than in controls (2304.50±644.15 pg/mL) (p<0.0001). LTA4H recessive variants influence protein levels (functional genetics), resulting in risk of EPTB.
{"title":"Association of Leukotriene A4 Hydrolase polymorphisms and risk of extrapulmonary tuberculosis","authors":"Pooja Singh Gaur, Surya Kant, Rachna Chaturvedi","doi":"10.25303/1809rjbt1910197","DOIUrl":"https://doi.org/10.25303/1809rjbt1910197","url":null,"abstract":"Genetic risk assessment of tuberculosis development and genotypic variation influence the gene and protein expression of leukotriene A4 hydrolase (LTA4H) at various sites of tuberculosis. The relationship between the genotype and phenotype of LATH4A was investigated in relation to the genetic risk assessment of extrapulmonary tuberculosis (EPTB). This study included 137 patients with EPTB and age-sex-matched 137 normal controls of the same ethnicity. LTA4H genotyping was performed using restriction fragment polymorphisms (RFLP) and protein level was analyzed using enzyme-linked immunosorbent assay (ELISA). The LTAH4 (rs.197833A/G) AG (codominant) and GG (recessive) genotypes showed 1.86 and 1.88-fold higher risks (p=0.0004 and p<0.0001 respectively) of developing EPTB. LTA4H (rs.2660898 T/G) TG (codominant) and GG (recessive) genotypes significantly increased the risk (OR:1.69, p=0.0001 and OR;1.77 p=0.008 respectively) of EPTB. Similarly, LTA4H (rs. 2540474) AG (codominant) and GG (recessive) genotypes (OR; 1.58 95% CI:1.22- 2.04, p<0.0001; OR, 1.86; 95%CI 1.37-2.52, p<0.0001) increased the risk of developing ETTB. The LTB4 protein levels were significantly higher in EPTB cases (mean ± SD; 3096.10±1287.6 pg/mL) than in controls (2304.50±644.15 pg/mL) (p<0.0001). LTA4H recessive variants influence protein levels (functional genetics), resulting in risk of EPTB.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.25303/1809rjbt1820190
D. Rajput, I. Gogoi, S. Saikia, L.R. Saikia, P. Chetia
In the realm of floriculture, orchids have attracted a lot of interest for their alluring and distinctive morphological looks. In the present study, the musky smelling orchid Dendrobium moschatum was screened for its phytochemical and antioxidant property analysis followed by characterization and isolation of bioactive compounds. The plant contained high amount of total phenolic content (TPC) and total flavonoid content (TFC) of 21.86 ± 0.44 and 12.52 ± 0.23 respectively. The acetone extract of leaf of D. moschatum was evaluated using GC-MS, TLC and NMR spectroscopy and based on these, 2H-1-benzopyran-2-one also termed as coumarin was isolated as the main bioactive compound. The crude extract of the species showed satisfactory results by inhibiting the growth of Gram-positive Bacillus subtilis at MIC 0.78 mg/mL, but not so effective against Gram-negative Escherichia coli and Klebsiella pneumoniae. This signifies that the plant is valuable source of essential bioactive compounds with potential antibacterial activity which is not only advantageous for its conservation in natural habitat but also for the development of therapeutic alternates against common bacterial infections.
{"title":"Phytochemical and antioxidant analysis of musky smelling Dendrobium moschatum and screening of its antibacterial potential","authors":"D. Rajput, I. Gogoi, S. Saikia, L.R. Saikia, P. Chetia","doi":"10.25303/1809rjbt1820190","DOIUrl":"https://doi.org/10.25303/1809rjbt1820190","url":null,"abstract":"In the realm of floriculture, orchids have attracted a lot of interest for their alluring and distinctive morphological looks. In the present study, the musky smelling orchid Dendrobium moschatum was screened for its phytochemical and antioxidant property analysis followed by characterization and isolation of bioactive compounds. The plant contained high amount of total phenolic content (TPC) and total flavonoid content (TFC) of 21.86 ± 0.44 and 12.52 ± 0.23 respectively. The acetone extract of leaf of D. moschatum was evaluated using GC-MS, TLC and NMR spectroscopy and based on these, 2H-1-benzopyran-2-one also termed as coumarin was isolated as the main bioactive compound. The crude extract of the species showed satisfactory results by inhibiting the growth of Gram-positive Bacillus subtilis at MIC 0.78 mg/mL, but not so effective against Gram-negative Escherichia coli and Klebsiella pneumoniae. This signifies that the plant is valuable source of essential bioactive compounds with potential antibacterial activity which is not only advantageous for its conservation in natural habitat but also for the development of therapeutic alternates against common bacterial infections.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.25303/1809rjbt1110119
Sufiara Yousuf, Rahul Singh
Exploration of gut microbiota is a resource for researchers to study the range and role of micro-organisms. Exploring gut microbiota of fishes that survive in harsh environmental conditions, catches eye that opportunity of finding new strain may be more. The present study was undertaken to explore the gastrointestinal tract of adult Channa striatus collected from Doaba region of Punjab. A total of 34 isolates were isolated and screened for antimicrobial activity against fish pathogens: Aeromonas hydrophila and Pseudomonas aeruginosa. Four of these isolates were Gram positive. Of these four isolates, only one isolate PMS1 showed good antimicrobial activity against the Aeromonas hydrophila. The isolate exhibited high tolerance to low pH and strong adhesion capability. The isolate was found susceptible to all tested antibiotics. Based on in vitro assessment, biochemical and 16srRNA analysis the strain was identified as Lactococcus garvieae. Furthermore, extracellular metabolites were isolated and analyzed through Gas Chromatography-Mass Spectrometry technique. The results revealed the presence of two compounds (1, 3, 5, 7, 9, 11- Hexaethylcyclohexasiloxane and 2, 6 Lutidine 3, 5 dichloro-4- dodecylthio-). This study concludes that the isolate showed positive in vitro results and the metabolites do not confirm the pathogenicity of the strain. The findings suggest that further studies are required to evaluate the effect of Lactococcus garvieae PMS1 in fish.
{"title":"Analysis of the ability of Channa striatus gut-derived Lactococcus garvieae bacteria to reach and persist in the gut environment along with extracellular metabolites and its behavior against common fish pathogens","authors":"Sufiara Yousuf, Rahul Singh","doi":"10.25303/1809rjbt1110119","DOIUrl":"https://doi.org/10.25303/1809rjbt1110119","url":null,"abstract":"Exploration of gut microbiota is a resource for researchers to study the range and role of micro-organisms. Exploring gut microbiota of fishes that survive in harsh environmental conditions, catches eye that opportunity of finding new strain may be more. The present study was undertaken to explore the gastrointestinal tract of adult Channa striatus collected from Doaba region of Punjab. A total of 34 isolates were isolated and screened for antimicrobial activity against fish pathogens: Aeromonas hydrophila and Pseudomonas aeruginosa. Four of these isolates were Gram positive. Of these four isolates, only one isolate PMS1 showed good antimicrobial activity against the Aeromonas hydrophila. The isolate exhibited high tolerance to low pH and strong adhesion capability. The isolate was found susceptible to all tested antibiotics. Based on in vitro assessment, biochemical and 16srRNA analysis the strain was identified as Lactococcus garvieae. Furthermore, extracellular metabolites were isolated and analyzed through Gas Chromatography-Mass Spectrometry technique. The results revealed the presence of two compounds (1, 3, 5, 7, 9, 11- Hexaethylcyclohexasiloxane and 2, 6 Lutidine 3, 5 dichloro-4- dodecylthio-). This study concludes that the isolate showed positive in vitro results and the metabolites do not confirm the pathogenicity of the strain. The findings suggest that further studies are required to evaluate the effect of Lactococcus garvieae PMS1 in fish.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Quorum sensing system majorly contributes in the virulence of many microorganisms. Attenuation in the quorum sensing system by interaction with the signalling molecules can be an effective approach to reduce the use of anti-microbial agents. The aim of the study is to investigate quorum sensing inhibitory activity of microbes from the marine source. A total of 60 epibiotic bacteria were isolated: 4 isolates were anti-QS producer and one of them with potent anti-QS activity was identified as Pseudomonas stutzeri SJ4. The ethyl acetate extract of P. stutzeri SJ4 inhibited violacein pigment of Chromobacterium violaceum by 72.83%. To characterise the active compound, TLC and GC-MS were performed. GC-MS showed major 5 peaks whose compounds were docked with the Quorum sensing receptor CviR of C.violaceum. Molecular docking results indicated that 2,6,10,14- tetramethyl hexadecane showed highest binding energy -8.0 kcal/mol which is quite higher than control compound N-Decanoyl-DL-homoserine lactone, exhibiting -7.5 kcal/mol.
{"title":"Inhibition of quorum sensing by partially purified anti-quorum sensing compound from the marine epibiotic bacteria in Chromobacterium violaceum","authors":"Siddhi Shah, Harsh Patel, Bhakti Bajpai","doi":"10.25303/1809rjbt011017","DOIUrl":"https://doi.org/10.25303/1809rjbt011017","url":null,"abstract":"Quorum sensing system majorly contributes in the virulence of many microorganisms. Attenuation in the quorum sensing system by interaction with the signalling molecules can be an effective approach to reduce the use of anti-microbial agents. The aim of the study is to investigate quorum sensing inhibitory activity of microbes from the marine source. A total of 60 epibiotic bacteria were isolated: 4 isolates were anti-QS producer and one of them with potent anti-QS activity was identified as Pseudomonas stutzeri SJ4. The ethyl acetate extract of P. stutzeri SJ4 inhibited violacein pigment of Chromobacterium violaceum by 72.83%. To characterise the active compound, TLC and GC-MS were performed. GC-MS showed major 5 peaks whose compounds were docked with the Quorum sensing receptor CviR of C.violaceum. Molecular docking results indicated that 2,6,10,14- tetramethyl hexadecane showed highest binding energy -8.0 kcal/mol which is quite higher than control compound N-Decanoyl-DL-homoserine lactone, exhibiting -7.5 kcal/mol.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.25303/1809rjbt2310246
Nithiyanandam Sangeetha, Katturajan Ramkumar, Evan Prince Sabina
Drug-instigated toxicity is a leading source of organ injury due to apoptosis and inflammation. It is still a serious issue that demands the development of successful drugs to treat and prevent such organ damage. Herbal remedies are effective in the treatment of organ toxicity. Thus, the objective of this current study emphasizes scrutinizing interactions between phytoactive compounds of Caesalpinia bonducella seeds and targeted proteins of the apoptosis and inflammatory pathway. The SwitssADME online server was used to screen the pharmacokinetics and molecular properties of phytoactive compounds from Caesalpinia bonducella seeds. The PASS program was employed to assess phytoactive compounds' anti-apoptosis and anti-inflammatory activity. Using AutoDock Vina software, the ligands were docked onto targeted apoptotic and inflammatory proteins. The protein-ligand interaction was analyzed and visualized using Discovery studio software. The two most efficient regulators were glyceryl monostearate and 2-Palmitoylglycerol which interacted strongly with the binding sites of all the targeted proteins of apoptosis and inflammatory pathways. Glyceryl monostearate and 2-Palmitoylglycerol could be potential regulators for mitigating the inflammation and apoptosis instigated by therapeutical drug toxicity. They have the potency to be used as anti-apoptotic and anti-inflammatory agents and are intriguing research candidates for further research.
{"title":"Scrutinizing Caesalpinia bonducella phytoactive compounds as potent regulators targeting proteins of drug instigated apoptotic and inflammatory pathways: An in-silico approach","authors":"Nithiyanandam Sangeetha, Katturajan Ramkumar, Evan Prince Sabina","doi":"10.25303/1809rjbt2310246","DOIUrl":"https://doi.org/10.25303/1809rjbt2310246","url":null,"abstract":"Drug-instigated toxicity is a leading source of organ injury due to apoptosis and inflammation. It is still a serious issue that demands the development of successful drugs to treat and prevent such organ damage. Herbal remedies are effective in the treatment of organ toxicity. Thus, the objective of this current study emphasizes scrutinizing interactions between phytoactive compounds of Caesalpinia bonducella seeds and targeted proteins of the apoptosis and inflammatory pathway. The SwitssADME online server was used to screen the pharmacokinetics and molecular properties of phytoactive compounds from Caesalpinia bonducella seeds. The PASS program was employed to assess phytoactive compounds' anti-apoptosis and anti-inflammatory activity. Using AutoDock Vina software, the ligands were docked onto targeted apoptotic and inflammatory proteins. The protein-ligand interaction was analyzed and visualized using Discovery studio software. The two most efficient regulators were glyceryl monostearate and 2-Palmitoylglycerol which interacted strongly with the binding sites of all the targeted proteins of apoptosis and inflammatory pathways. Glyceryl monostearate and 2-Palmitoylglycerol could be potential regulators for mitigating the inflammation and apoptosis instigated by therapeutical drug toxicity. They have the potency to be used as anti-apoptotic and anti-inflammatory agents and are intriguing research candidates for further research.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An in vitro regeneration system was standarized on a traditional medicinal plant of Kaempferia parviflora. The rhizome sprouted buds upon transfer to MS basal medium with kinetin (3mg/l) and naphthalene acetic acid (0.5mg/l) were found optimum exhibiting maximum percent of shoot initiation as 90.6 ± 0.2. Maximum number of shoots as 8.1 ± 0.3 and maximum number of roots as 22.0 ± 0.3 were found in benzyladenine (3mg/l), naphthalene acetic acid (1mg/l) and thidiazuron (0.5mg/l) combination. Then the micropropagated plants were transferred to field for successful establishment as 90%. Furthermore, the morphological characteristic analysis was done between the conventional and micropropagated plants which showed very less significant variation. Thus the present protocol reports the propagation of K. parviflora plantlets which could be used for commercial exploitation in future.
{"title":"Influence of Thidiazuron on in vitro regeneration potential of Kaempferia parviflora Wall. Ex Baker from Eastern India","authors":"Rajashree Panigrahi, Reena Parida","doi":"10.25303/1809rjbt018022","DOIUrl":"https://doi.org/10.25303/1809rjbt018022","url":null,"abstract":"An in vitro regeneration system was standarized on a traditional medicinal plant of Kaempferia parviflora. The rhizome sprouted buds upon transfer to MS basal medium with kinetin (3mg/l) and naphthalene acetic acid (0.5mg/l) were found optimum exhibiting maximum percent of shoot initiation as 90.6 ± 0.2. Maximum number of shoots as 8.1 ± 0.3 and maximum number of roots as 22.0 ± 0.3 were found in benzyladenine (3mg/l), naphthalene acetic acid (1mg/l) and thidiazuron (0.5mg/l) combination. Then the micropropagated plants were transferred to field for successful establishment as 90%. Furthermore, the morphological characteristic analysis was done between the conventional and micropropagated plants which showed very less significant variation. Thus the present protocol reports the propagation of K. parviflora plantlets which could be used for commercial exploitation in future.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-15DOI: 10.25303/1809rjbt1470154
Mukesh Verma, Baljeet Singh Saharan, Anil Kumar
The present study was carried out to evaluate the hypoglycemic ability and antioxidant activities of lactic acid bacterial strain L. acidophilus NCDC 291 and to investigate the probiotic characteristics of the strain as well as its suitability as an alternative antimutagenic agent. The antidiabetic activity was examined by evaluating the α-glucosidase and α-amylase inhibitory activities. Strain L. acidophilus NCDC 291 exhibited hypoglycemic ability by α-glucosidase (41.67 %) and α-amylase (61.79 %). Strain L. acidophilus NCDC 291 showed antioxidant activities by DPPH (53.58 % and IC50 67.87 μL) and ABTS (50.61 % and IC50 45.17 μL). Further the bacterial strain was resistant to simulated gastrointestinal juice and showed potential for health promotion based on adhesion, antibiotic susceptibility and antimicrobial activities. L. acidophilus NCDC 291 appears as potential probiotic with beneficial health effects.
{"title":"Contributions of probiotic L. acidophilus 291 as biotechnological tool in therapeutic applications","authors":"Mukesh Verma, Baljeet Singh Saharan, Anil Kumar","doi":"10.25303/1809rjbt1470154","DOIUrl":"https://doi.org/10.25303/1809rjbt1470154","url":null,"abstract":"The present study was carried out to evaluate the hypoglycemic ability and antioxidant activities of lactic acid bacterial strain L. acidophilus NCDC 291 and to investigate the probiotic characteristics of the strain as well as its suitability as an alternative antimutagenic agent. The antidiabetic activity was examined by evaluating the α-glucosidase and α-amylase inhibitory activities. Strain L. acidophilus NCDC 291 exhibited hypoglycemic ability by α-glucosidase (41.67 %) and α-amylase (61.79 %). Strain L. acidophilus NCDC 291 showed antioxidant activities by DPPH (53.58 % and IC50 67.87 μL) and ABTS (50.61 % and IC50 45.17 μL). Further the bacterial strain was resistant to simulated gastrointestinal juice and showed potential for health promotion based on adhesion, antibiotic susceptibility and antimicrobial activities. L. acidophilus NCDC 291 appears as potential probiotic with beneficial health effects.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135162891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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