Pub Date : 2025-08-18DOI: 10.1016/j.ram.2025.07.002
Jesús Rafael Trinidad-Cruz, Gabriel Rincón-Enríquez, Zahaed Evangelista-Martínez, Evangelina Esmeralda Quiñones-Aguilar
Species of the genus Streptomyces are a promising strategy for bacterial disease management in agriculture crops. The present study aimed to isolate and identify Streptomyces-like actinobacteria from rhizospheric soil using physical pretreatments and to evaluate their antimicrobial activity against Xanthomonas sp. A rhizospheric soil from a bean plantation was pretreated using dry heat or microwave radiation for isolating actinobacteria. Antimicrobial activity was evaluated using the double agar layer method; 73 isolates exhibiting Streptomyces colony characteristics were obtained from the soil after dry heat pre-treatment series (50 BVBZ) and microwaves (23 BVBZMW); 34 BVBZ (68%) isolates inhibited Xanthomonas sp. growth with 11.2-35.8mm halos in diameter. Fifteen (15) of 23 BVBZMW isolates (65%) recorded inhibition zones ranging from 15.7 to 73.6mm in diameter. The 16S rRNA gene sequence analysis confirmed three isolates with greater antimicrobial activity belonging to the genus Streptomyces. Streptomyces sp. BVBZ 35, BVBZ 47, and BVBZMW 18 shared greater 16S rRNA gene sequence identity with S. monomycini NRRL B-2409T (100%), S. nogalater JCM 4799T (99.38%) and S. leeuwenhoekii C34T (99.45%), respectively. Streptomyces sp. BVBZMW 18, which exhibited the highest antimicrobial activity, could only be cultured after microwave irradiation of the soil. Streptomyces species isolated from rhizospheric soil using physical pretreatments are a potentially novel antimicrobial source for Xanthomonas disease control.
{"title":"Antimicrobial activity of Streptomyces spp. isolated from bean rhizospheric soil against Xanthomonas sp.","authors":"Jesús Rafael Trinidad-Cruz, Gabriel Rincón-Enríquez, Zahaed Evangelista-Martínez, Evangelina Esmeralda Quiñones-Aguilar","doi":"10.1016/j.ram.2025.07.002","DOIUrl":"10.1016/j.ram.2025.07.002","url":null,"abstract":"<p><p>Species of the genus Streptomyces are a promising strategy for bacterial disease management in agriculture crops. The present study aimed to isolate and identify Streptomyces-like actinobacteria from rhizospheric soil using physical pretreatments and to evaluate their antimicrobial activity against Xanthomonas sp. A rhizospheric soil from a bean plantation was pretreated using dry heat or microwave radiation for isolating actinobacteria. Antimicrobial activity was evaluated using the double agar layer method; 73 isolates exhibiting Streptomyces colony characteristics were obtained from the soil after dry heat pre-treatment series (50 BVBZ) and microwaves (23 BVBZMW); 34 BVBZ (68%) isolates inhibited Xanthomonas sp. growth with 11.2-35.8mm halos in diameter. Fifteen (15) of 23 BVBZMW isolates (65%) recorded inhibition zones ranging from 15.7 to 73.6mm in diameter. The 16S rRNA gene sequence analysis confirmed three isolates with greater antimicrobial activity belonging to the genus Streptomyces. Streptomyces sp. BVBZ 35, BVBZ 47, and BVBZMW 18 shared greater 16S rRNA gene sequence identity with S. monomycini NRRL B-2409<sup>T</sup> (100%), S. nogalater JCM 4799<sup>T</sup> (99.38%) and S. leeuwenhoekii C34<sup>T</sup> (99.45%), respectively. Streptomyces sp. BVBZMW 18, which exhibited the highest antimicrobial activity, could only be cultured after microwave irradiation of the soil. Streptomyces species isolated from rhizospheric soil using physical pretreatments are a potentially novel antimicrobial source for Xanthomonas disease control.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-24DOI: 10.1016/j.ram.2025.06.003
Lucía Rago, Gabriela Rubinstein, Carlos Federico Spinelli, María Sol Haim, Tomás Poklépovich Caride, Sabrina Di Gregorio, Marta Mollerach
In this study, we describe two closely related cases of multifocal MSSA infections within the same household. The dissemination of infection was severe in both cases, but none of the patients presented endocarditis. All isolates shared resistance to erythromycin and clindamycin (inducible macrolide resistance phenotype, iMLSb). The genomic analysis of one of the isolates indicated that it belongs to the human-associated CC398-MSSA clonal complex, an emerging lineage in our region. Whole genome sequencing enabled the characterization and differentiation of this clone from the livestock-associated CC398-MSSA lineage.
{"title":"Severe disseminated nfections in cohabiting people: Genotypic description of methicillin-susceptible Staphylococcus aureus (MSSA) isolate belonging to CC398 clonal complex.","authors":"Lucía Rago, Gabriela Rubinstein, Carlos Federico Spinelli, María Sol Haim, Tomás Poklépovich Caride, Sabrina Di Gregorio, Marta Mollerach","doi":"10.1016/j.ram.2025.06.003","DOIUrl":"https://doi.org/10.1016/j.ram.2025.06.003","url":null,"abstract":"<p><p>In this study, we describe two closely related cases of multifocal MSSA infections within the same household. The dissemination of infection was severe in both cases, but none of the patients presented endocarditis. All isolates shared resistance to erythromycin and clindamycin (inducible macrolide resistance phenotype, iMLSb). The genomic analysis of one of the isolates indicated that it belongs to the human-associated CC398-MSSA clonal complex, an emerging lineage in our region. Whole genome sequencing enabled the characterization and differentiation of this clone from the livestock-associated CC398-MSSA lineage.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This report presented the case of a 55-year-old patient who was admitted to the intensive care unit following a ruptured bronchial arteriovenous malformation (AVM). The patient, with no significant medical history, experienced massive hemoptysis leading to cardiac arrest. After successful resuscitation and embolization of the AVM, the patient developed fever and acute respiratory distress syndrome, requiring deep sedation, muscle paralysis, and prone positioning. Blood culture sampled on the day of admission tested positive for Lancefieldella parvula on day 4. The patient received targeted antimicrobial therapy, resulting in a favorable hemodynamic and respiratory outcome. No source of the bacteremia was found. We reported the general management of this rare cause of bacteremia and provided a review of the existing literature.
{"title":"Clinical management of Lancefieldella parvula bacteremia: A case study and review of the literature.","authors":"Cynthia Wenglinski, Delphine Martiny, Michael Watchi, Fabio Silvio Taccone, Charles-Hervé Vacheron","doi":"10.1016/j.ram.2025.06.004","DOIUrl":"https://doi.org/10.1016/j.ram.2025.06.004","url":null,"abstract":"<p><p>This report presented the case of a 55-year-old patient who was admitted to the intensive care unit following a ruptured bronchial arteriovenous malformation (AVM). The patient, with no significant medical history, experienced massive hemoptysis leading to cardiac arrest. After successful resuscitation and embolization of the AVM, the patient developed fever and acute respiratory distress syndrome, requiring deep sedation, muscle paralysis, and prone positioning. Blood culture sampled on the day of admission tested positive for Lancefieldella parvula on day 4. The patient received targeted antimicrobial therapy, resulting in a favorable hemodynamic and respiratory outcome. No source of the bacteremia was found. We reported the general management of this rare cause of bacteremia and provided a review of the existing literature.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-10DOI: 10.1016/j.ram.2025.04.005
Adriana Efron, Luciana Moreira, Federico Lorenzo, Denise De Belder, María Moscoloni, Alejandra Corso, Mauricio Santos, Tomás Poklepovich, Josefina Campos, Paula Gagetti, María Sol Haim
Invasive meningococcal disease is associated with high fatality rates and disabling sequelae. The aims of this study were to describe the phenotypic and genotypic characteristics of invasive Neisseria meningitidis isolates in Argentina, determine the genomic population structure and evaluate the potential coverage of vaccines targeting N. meningitidis serogroup B (MenB). During the period 2015-2022, a total of 444 isolates of N. meningitidis causing invasive disease were assessed through laboratory surveillance; 344 isolates were available for whole genome sequencing characterization. The presence of antimicrobial resistance genes, and the distribution of clonal complexes and vaccine antigens were analyzed. MenB was the most frequent serogroup (53.2%), followed by MenW (32.0%). Most isolates were susceptible to the antimicrobial agents tested; however, 56.7% exhibited intermediate resistance to penicillin. MenB showed wide genetic diversity and was mainly associated with ST-865 cc, ST-35 cc, ST-41/44 cc, and ST-32 cc. Unlike other countries, in Argentina, ST-865 cc was one of the major clonal complexes associated with MenB, MenW was only associated with ST-11 cc. To study the potential coverage of vaccines targeting MenB, we used the MenDeVAR (Meningococcal Deduced Vaccine Antigen Reactivity) index, which provides information on the presence and possible immunological cross-reactivity of the different vaccine antigenic variants. MenDeVar showed 19.3% vaccine reactivity for 4CMenB and 40.0% for the bivalent vaccine. Laboratory surveillance is essential for generating evidence-based decisions for updating vaccination strategies.
{"title":"Phenotypic and genomic surveillance of invasive Neisseria meningitidis isolates in Argentina (2015-2022): Clonal structure and vaccine implications.","authors":"Adriana Efron, Luciana Moreira, Federico Lorenzo, Denise De Belder, María Moscoloni, Alejandra Corso, Mauricio Santos, Tomás Poklepovich, Josefina Campos, Paula Gagetti, María Sol Haim","doi":"10.1016/j.ram.2025.04.005","DOIUrl":"https://doi.org/10.1016/j.ram.2025.04.005","url":null,"abstract":"<p><p>Invasive meningococcal disease is associated with high fatality rates and disabling sequelae. The aims of this study were to describe the phenotypic and genotypic characteristics of invasive Neisseria meningitidis isolates in Argentina, determine the genomic population structure and evaluate the potential coverage of vaccines targeting N. meningitidis serogroup B (MenB). During the period 2015-2022, a total of 444 isolates of N. meningitidis causing invasive disease were assessed through laboratory surveillance; 344 isolates were available for whole genome sequencing characterization. The presence of antimicrobial resistance genes, and the distribution of clonal complexes and vaccine antigens were analyzed. MenB was the most frequent serogroup (53.2%), followed by MenW (32.0%). Most isolates were susceptible to the antimicrobial agents tested; however, 56.7% exhibited intermediate resistance to penicillin. MenB showed wide genetic diversity and was mainly associated with ST-865 cc, ST-35 cc, ST-41/44 cc, and ST-32 cc. Unlike other countries, in Argentina, ST-865 cc was one of the major clonal complexes associated with MenB, MenW was only associated with ST-11 cc. To study the potential coverage of vaccines targeting MenB, we used the MenDeVAR (Meningococcal Deduced Vaccine Antigen Reactivity) index, which provides information on the presence and possible immunological cross-reactivity of the different vaccine antigenic variants. MenDeVar showed 19.3% vaccine reactivity for 4CMenB and 40.0% for the bivalent vaccine. Laboratory surveillance is essential for generating evidence-based decisions for updating vaccination strategies.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.1016/j.ram.2025.07.001
Andrea Pontoriero , Maria Laura Minassian
{"title":"The re-emergence of vaccine-preventable diseases: The case of measles","authors":"Andrea Pontoriero , Maria Laura Minassian","doi":"10.1016/j.ram.2025.07.001","DOIUrl":"10.1016/j.ram.2025.07.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 205-207"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.1016/j.ram.2025.02.001
Wei Lu, Zong-long Li, De-yong Xu, Guo-ping Yu
The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of Bacteroidetes to that of Firmicutes (the B/F index) in the anorexia group was higher than in the control group. The abundance of Bacteroidetes in the anorexia group was higher than that in the control group, and the abundance of Actinomycetes in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were Bacteroidetes and Actinobacteria in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were Bacteroides, Faecalibacterium, and Subdoligranulum, and Bifidobacterium, Blautia, Streptococcus, Lachnoclostridium, and Erysipelatoclostridium in the control group. We conclude that the increase in Bacteroides abundance or in the B/F index and the reduction in Bifidobacterium abundance were related to the pathogenesis of anorexia.
{"title":"Analysis of intestinal microbiota diversity in children with non-organic anorexia","authors":"Wei Lu, Zong-long Li, De-yong Xu, Guo-ping Yu","doi":"10.1016/j.ram.2025.02.001","DOIUrl":"10.1016/j.ram.2025.02.001","url":null,"abstract":"<div><div>The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of <em>Bacteroidetes</em> to that of <em>Firmicutes</em> (the B/F index) in the anorexia group was higher than in the control group. The abundance of <em>Bacteroidetes</em> in the anorexia group was higher than that in the control group, and the abundance of <em>Actinomycetes</em> in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were <em>Bacteroidetes</em> and <em>Actinobacteria</em> in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were <em>Bacteroides</em>, <em>Faecalibacterium</em>, and <em>Subdoligranulum</em>, and <em>Bifidobacterium</em>, <em>Blautia</em>, <em>Streptococcus</em>, <em>Lachnoclostridium</em>, and <em>Erysipelatoclostridium</em> in the control group. We conclude that the increase in <em>Bacteroides</em> abundance or in the B/F index and the reduction in <em>Bifidobacterium</em> abundance were related to the pathogenesis of anorexia.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 221-230"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.1016/j.ram.2025.05.002
Natalia Andrea Di Clemente , Marina Peluffo , Ana Carolina Agnello , Deborah Colman , Norma Buceta , María Teresa Del Panno , Mario Carlos Nazareno Saparrat
Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.
{"title":"Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota","authors":"Natalia Andrea Di Clemente , Marina Peluffo , Ana Carolina Agnello , Deborah Colman , Norma Buceta , María Teresa Del Panno , Mario Carlos Nazareno Saparrat","doi":"10.1016/j.ram.2025.05.002","DOIUrl":"10.1016/j.ram.2025.05.002","url":null,"abstract":"<div><div>Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 288-297"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.1016/j.ram.2025.06.001
Carlos A. Rossetti , Ursula A. Rossi
{"title":"Genetic resistance to infectious diseases: A complementary tool to antimicrobial drugs in livestock?","authors":"Carlos A. Rossetti , Ursula A. Rossi","doi":"10.1016/j.ram.2025.06.001","DOIUrl":"10.1016/j.ram.2025.06.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 203-204"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.1016/j.ram.2024.12.001
Rodrigo Pereyra , Fernando Martino , Mariángeles Castillo , Juan Manuel Sala , Lucas José Barone , Claudio Paolazzi , Alejandra Victoria Capozzo
Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (p < 0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.
{"title":"Reporte de una experiencia de control del virus de la diarrea viral bovina en 2 tambos de Argentina aplicando herramientas de manejo, diagnóstico y vacunación","authors":"Rodrigo Pereyra , Fernando Martino , Mariángeles Castillo , Juan Manuel Sala , Lucas José Barone , Claudio Paolazzi , Alejandra Victoria Capozzo","doi":"10.1016/j.ram.2024.12.001","DOIUrl":"10.1016/j.ram.2024.12.001","url":null,"abstract":"<div><div>Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (<em>p</em> <!--><<!--> <!-->0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 241-250"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.1016/j.ram.2025.01.002
Mario Alberto Dueñas-Espinosa , Julio César López-Valdés , Daniel Alejandro Vega-Moreno , María Fernanda Guzmán-del Río , Rafael Sánchez-Mata , María Elena Córdoba-Mosqueda , Laura Mestre-Orozco , Erick Alberto Castañeda-Ramírez , Alejandro Jacob Madrid-Sánchez , Ulises García-González
The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's t-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0 ± 11.5 years (range: 38–83), and the average body mass index (BMI) was 28 ± 4.05 kg/m2. Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were Staphylococcus aureus (28 cases) and Escherichia coli (21 cases). In 16 cases, intracellular pathogens such as Mycobacterium tuberculosis and Brucella spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.
{"title":"Clinical and demographic characteristics of spondylodiscitis in a Mexican population: A retrospective study","authors":"Mario Alberto Dueñas-Espinosa , Julio César López-Valdés , Daniel Alejandro Vega-Moreno , María Fernanda Guzmán-del Río , Rafael Sánchez-Mata , María Elena Córdoba-Mosqueda , Laura Mestre-Orozco , Erick Alberto Castañeda-Ramírez , Alejandro Jacob Madrid-Sánchez , Ulises García-González","doi":"10.1016/j.ram.2025.01.002","DOIUrl":"10.1016/j.ram.2025.01.002","url":null,"abstract":"<div><div>The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's <em>t</em>-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0<!--> <!-->±<!--> <!-->11.5 years (range: 38–83), and the average body mass index (BMI) was 28<!--> <!-->±<!--> <!-->4.05<!--> <!-->kg/m<sup>2</sup>. Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were <em>Staphylococcus aureus</em> (28 cases) and <em>Escherichia coli</em> (21 cases). In 16 cases, intracellular pathogens such as <em>Mycobacterium tuberculosis</em> and <em>Brucella</em> spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 256-264"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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