Revista Argentina de microbiologia最新文献

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Detection of Campylobacter spp. and Yersina spp. in chicken burgers: Critical evaluation of CIN medium and characterization of psychrotolerant microbiota in commercial samples from Reus (Tarragona, Spain) 鸡肉汉堡中弯曲杆菌和耶尔西那氏杆菌的检测：来自Reus （Tarragona, Spain）的商业样品中CIN培养基的关键评估和耐寒微生物群的表征。

IF 2.1 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-10-01 Epub Date: 2025-07-22 DOI: 10.1016/j.ram.2025.06.002

Marta Sanchis , Laura Camuña-Pardo , Laia Martínez Quijada , Altea Pérez Escobar , Frederic Gómez-Bertomeu , Alberto Miguel Stchigel Glikman

Two of the three most prevalent bacterial genera associated with human diseases caused by meat consumption are Campylobacter (mainly in poultry meat) and Yersinia (in pork meat). As its detection and quantification is not regulated by the European legislation (except for the quantification of Campylobacter in poultry channels), several samples of chicken burgers from different establishments including supermarkets and retail trade outlets in Reus (Spain) were processed to ensure microbiological safety. Microbiological criteria and procedures have been those included in the European standards for Campylobacter (Regulation No. 2017/1495) and Yersinia (ISO 10273:2917). Results showed the absence of Campylobacter spp. in all samples, but high counts (10⁴ to 5 × 10⁵ CFU/g) of typical colonies (“red bull's eye” morphology) on CIN medium compatible with Yersinia spp. The biochemical profile of the strains from the typical colonies, and their subsequent molecular identification using MALDI-TOF MS enabled us to identify Yersinia intermedia in just one sample, and Pseudomonas and Serratia liquefaciens in the remaining ones. These findings call into question the usefulness of CIN medium for detecting Yersinia spp. in food. Moreover, the presence of high counts of psychrotolerant bacteria from the genera Pseudomonas and Serratia highlight the need to improve hygienic conditions in the procedures used to produce meat derivatives.

与肉类消费引起的人类疾病相关的三种最普遍的细菌属中有两种是弯曲杆菌（主要存在于禽肉中）和耶尔森氏菌（存在于猪肉中）。由于其检测和定量不受欧洲立法的管制（家禽渠道中的弯曲杆菌的定量除外），因此对来自Reus（西班牙）不同场所（包括超市和零售商店）的几个鸡肉汉堡样本进行了处理，以确保微生物安全。微生物标准和程序已纳入欧洲弯曲杆菌（法规2017/1495）和耶尔森氏菌（ISO 10273:2917）标准。结果显示，所有样品中都没有弯曲杆菌，但在与耶尔森氏菌相容的CIN培养基上，典型菌落（“红牛眼”形态）的计数很高（104至5×105CFU/g）。典型菌落的生物化学特征以及随后使用MALDI-TOF MS进行的分子鉴定使我们能够在一个样品中鉴定出耶尔森氏菌，在其余样品中鉴定出假单胞菌和液化沙利菌。这些发现对CIN培养基检测食物中耶尔森氏菌的有效性提出了质疑。此外，来自假单胞菌属和沙雷氏菌属的高耐寒细菌的存在突出表明需要改善用于生产肉类衍生物的程序的卫生条件。

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引用次数: 0

Variación de la microbiota oral facultativa durante períodos de estrés académico [学业压力期间兼性口腔微生物群的变化]。

IF 2.1 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-10-01 Epub Date: 2025-10-16 DOI: 10.1016/j.ram.2025.09.005

Victor Ludeña-Meléndez , Jussara Malo-Castillo , Paula Ishikawa-Arias , Harold Jimenez-Alvarez , Criss Ocampo , Cristhian Guevara-Coronel , Leonardo Ledesma Chavarria , Meilyn Lujan-Benites , Cristhian Terrones , Christian Eduardo Vilca Avalos , Maria Soledad Ayala Ravelo

Academic stress is a factor that impacts the health of medical students by altering their body's internal environment and its various constituent systems, including the microbiota of the digestive system, particularly that of the oral cavity. Therefore, the objective of this study was to evaluate the variation in the facultative oral microbiota of medical students during periods of perceived academic stress during evaluation periods. This was an observational, analytical study that used mouthwash samples and microbiological colony-forming units counts per ml (CFU/ml) of oral bacterial and fungal microorganisms. Forty-seven participants were included: 59.57% were male, 55.32% had good oral hygiene habits, and 93.62% belonged to a middle or low socioeconomic status. The academic stress score was higher in response to the stressful event (UAE = 62.5 ± 12.3) and was significantly associated with a higher CFU/ml count (p <0.05). Yeast colonies corresponding to Candida albicans, C. tropicalis, C. glabrata, and Candida spp. were identified on CHROMagar^TM. It is concluded that there is an association between perceived academic stress and socioeconomic status with the variation in the bacterial and fungal oral microbiota, respectively.

学业压力是影响医学生健康的一个因素，它改变了医学生身体的内部环境和各种组成系统，包括消化系统的微生物群，尤其是口腔的微生物群。因此，本研究的目的是评估医学生在评估期间感受到学业压力时兼性口腔微生物群的变化。这是一项观察性分析性研究，使用漱口水样本和口腔细菌和真菌微生物每毫升微生物菌落形成单位计数（CFU/ml）。其中男性占59.57%，口腔卫生习惯良好者占55.32%，社会经济地位中低者占93.62%。应激事件后学业压力得分较高（UAE=62.5±12.3），且与CFU/ml计数显著相关（p TM）。由此可见，学业压力和社会经济地位分别与口腔细菌菌群和真菌菌群的变化有关。

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引用次数: 0

Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae) 苏云金芽孢杆菌菌株对小粉虫甲（鞘翅目：拟粉虫科）的毒力评价。

IF 2.1 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-10-01 Epub Date: 2025-05-22 DOI: 10.1016/j.ram.2025.03.003

Melisa P. Pérez , Graciela B. Benintende , Diego H. Sauka

This study addresses the pervasive challenge of lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) infestations in poultry farming. Our aim was to select toxic Bacillus thuringiensis strains against A. diaperinus larvae and determine the fraction(s) within the bacterial cultures harboring the active metabolites responsible for this insecticidal activity. Among the 41 strains evaluated, the Argentine strain INTA Mo4-4 showed the highest toxicity. Bioassays revealed that the main virulence factors reside within the spore-crystal pellet associated with the Cry protein. These results set the basis for the development of a targeted bioinsecticide. Further research is needed to elucidate the underlying mechanisms and validate these strategies in field trials. By harnessing B. thuringiensis-based bioinsecticides, we offer a sustainable solution to mitigate A. diaperinus infestations.

本研究解决了家禽养殖中普遍存在的小粉虫（鞘翅目：拟粉虫科）侵染问题。我们的目的是选择有毒的苏云金芽孢杆菌菌株，以对抗甲鼠幼虫，并确定细菌培养物中含有活性代谢物的比例，这些代谢物负责这种杀虫活性。41株毒株中，阿根廷菌株INTA Mo4-4毒性最高。生物分析表明，主要的毒力因子存在于与Cry蛋白相关的孢子晶体颗粒中。这些结果为开发靶向生物杀虫剂奠定了基础。需要进一步的研究来阐明潜在的机制并在田间试验中验证这些策略。通过利用苏云金芽孢杆菌为基础的生物杀虫剂，我们提供了一种可持续的解决方案，以减轻尿螨的侵扰。

引用次数: 0

Mosquito-borne pathogens … Now Rickettsias? 蚊子传播的病原体…现在是立克次体？

IF 2.1 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-10-01 Epub Date: 2025-05-26 DOI: 10.1016/j.ram.2025.03.002

Silvina Goenaga , Estefania Raquel Boaglio , Lucia Victoria Martinez , Juliana Patricia Sanchez

In the last few decades, the emergence of several infectious diseases has increased. Mosquitoes are the most important vectors due to their role in transmitting several pathogens, such as dengue, chikungunya and Plasmodium spp. Recently, mosquitoes have been suspected of transmitting Rickettsias, another emerging pathogen. However, their role in the transmission of this agent remains unclear. In this study, we report the detection of a Rickettsia spp. in Coquillettidia venezuelensis mosquitoes collected from Corrientes Province, Argentina. The phylogenetic analyses show that this Rickettsia is related to Rickettsia felis, and the ompB sequence shows high homology with Rickettsia tillamookensis. The evidence of the detection of Rickettsia in mosquitoes shows that several infectious diseases could be transmitted by mosquitoes, and the interaction between them is important to investigate. More studies should be done in order to analyze the impact on public health.

在过去的几十年里，几种传染病的出现有所增加。蚊子是传播登革热、基孔肯雅热和疟原虫等多种病原体的最重要媒介，最近，蚊子被怀疑传播另一种新兴病原体立克次体。然而，它们在这种媒介的传播中所起的作用仍不清楚。本研究报告在阿根廷科连特斯省采集的委内瑞拉Coquillettidia venezuela蚊中检出1株立克次体。系统发育分析表明，该立克次体与猫立克次体有亲缘关系，且ompB序列与蒂拉莫肯氏立克次体具有较高的同源性。在蚊子中检测立克次体的证据表明，几种传染病可以通过蚊子传播，它们之间的相互作用是研究的重要内容。为了分析对公众健康的影响，应该进行更多的研究。

引用次数: 0

The transmission of viral diseases in wild boar using Rutherford's atomic model 利用卢瑟福原子模型研究野猪病毒性疾病的传播

IF 2.1 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-10-01 Epub Date: 2025-11-27 DOI: 10.1016/j.ram.2025.11.001

Germán Ernesto Metz, María Gabriela Echeverría

引用次数: 0

Antimicrobial activity of Streptomyces spp. isolated from bean rhizospheric soil against Xanthomonas sp. 大豆根际土壤链霉菌对黄单胞菌的抑菌活性研究。

IF 2.1 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-08-18 DOI: 10.1016/j.ram.2025.07.002

Jesús Rafael Trinidad-Cruz, Gabriel Rincón-Enríquez, Zahaed Evangelista-Martínez, Evangelina Esmeralda Quiñones-Aguilar

Species of the genus Streptomyces are a promising strategy for bacterial disease management in agriculture crops. The present study aimed to isolate and identify Streptomyces-like actinobacteria from rhizospheric soil using physical pretreatments and to evaluate their antimicrobial activity against Xanthomonas sp. A rhizospheric soil from a bean plantation was pretreated using dry heat or microwave radiation for isolating actinobacteria. Antimicrobial activity was evaluated using the double agar layer method; 73 isolates exhibiting Streptomyces colony characteristics were obtained from the soil after dry heat pre-treatment series (50 BVBZ) and microwaves (23 BVBZMW); 34 BVBZ (68%) isolates inhibited Xanthomonas sp. growth with 11.2-35.8mm halos in diameter. Fifteen (15) of 23 BVBZMW isolates (65%) recorded inhibition zones ranging from 15.7 to 73.6mm in diameter. The 16S rRNA gene sequence analysis confirmed three isolates with greater antimicrobial activity belonging to the genus Streptomyces. Streptomyces sp. BVBZ 35, BVBZ 47, and BVBZMW 18 shared greater 16S rRNA gene sequence identity with S. monomycini NRRL B-2409^T (100%), S. nogalater JCM 4799^T (99.38%) and S. leeuwenhoekii C34^T (99.45%), respectively. Streptomyces sp. BVBZMW 18, which exhibited the highest antimicrobial activity, could only be cultured after microwave irradiation of the soil. Streptomyces species isolated from rhizospheric soil using physical pretreatments are a potentially novel antimicrobial source for Xanthomonas disease control.

链霉菌属是一种很有前途的农业作物细菌性病害管理策略。本研究旨在利用物理预处理方法从根际土壤中分离和鉴定链霉菌样放线菌，并评价其对黄单胞菌的抑菌活性。采用干热或微波预处理方法分离豆园根际土壤放线菌。采用双琼脂层法测定其抑菌活性；经干热预处理系列（50 BVBZ）和微波预处理系列（23 BVBZMW）从土壤中分离得到73株链霉菌菌落特征；34株BVBZ（68%）抑制黄单胞菌生长，光晕直径为11.2 ~ 35.8mm。23株BVBZMW菌株中15株（65%）的抑菌区直径在15.7 ~ 73.6mm之间。16S rRNA基因序列分析证实了3株具有较强抗菌活性的菌株属于链霉菌属。链霉菌sp. bvbz35、bvbz47和BVBZMW 18分别与S. monomycini NRRL B-2409T（100%）、S. nogalater JCM 4799T（99.38%）和S. leeuwenhoekii C34T（99.45%）具有较高的16S rRNA基因序列同源性。抗菌活性最高的Streptomyces sp. BVBZMW 18只能在土壤微波照射下培养。利用物理预处理从根际土壤中分离出的链霉菌是控制黄单胞菌病的一种潜在的新型抗菌来源。

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引用次数: 0

Severe disseminated nfections in cohabiting people: Genotypic description of methicillin-susceptible Staphylococcus aureus (MSSA) isolate belonging to CC398 clonal complex. 同居人群的严重播散性感染：属于CC398克隆复合体的甲氧西林敏感金黄色葡萄球菌（MSSA）分离物的基因型描述

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-24 DOI: 10.1016/j.ram.2025.06.003

Lucía Rago, Gabriela Rubinstein, Carlos Federico Spinelli, María Sol Haim, Tomás Poklépovich Caride, Sabrina Di Gregorio, Marta Mollerach

In this study, we describe two closely related cases of multifocal MSSA infections within the same household. The dissemination of infection was severe in both cases, but none of the patients presented endocarditis. All isolates shared resistance to erythromycin and clindamycin (inducible macrolide resistance phenotype, iMLSb). The genomic analysis of one of the isolates indicated that it belongs to the human-associated CC398-MSSA clonal complex, an emerging lineage in our region. Whole genome sequencing enabled the characterization and differentiation of this clone from the livestock-associated CC398-MSSA lineage.

在这项研究中，我们描述了两个密切相关的病例多灶MSSA感染在同一家庭。两例患者感染传播严重，但均未出现心内膜炎。所有分离株均对红霉素和克林霉素耐药（诱导型大环内酯耐药表型，iMLSb）。其中一个分离株的基因组分析表明，它属于人类相关的CC398-MSSA克隆复合体，这是我们地区一个新兴的谱系。全基因组测序使该克隆与家畜相关的CC398-MSSA谱系进行了表征和分化。

引用次数: 0

The re-emergence of vaccine-preventable diseases: The case of measles 疫苗可预防疾病的再次出现：麻疹病例

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-07-18 DOI: 10.1016/j.ram.2025.07.001

Andrea Pontoriero , Maria Laura Minassian

引用次数: 0

Analysis of intestinal microbiota diversity in children with non-organic anorexia 非器质性厌食症患儿肠道菌群多样性分析。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-04-09 DOI: 10.1016/j.ram.2025.02.001

Wei Lu, Zong-long Li, De-yong Xu, Guo-ping Yu

The pathogenesis of non-organic anorexia in children is not clear. This study intends to analyze intestinal bacteria to provide a relevant theoretical basis for the clinical rational selection of microecological agents. In the present study, children with non-organic anorexia were included in the anorexia group and normal healthy children in the control group. Stool samples were collected for the bioinformatics analysis after PCR and high-throughput sequencing. The results showed that the Ace, Chao, and Shannon indexes in the anorexia group were higher than those in the control group, while the Simpson index in the control group was lower than in the anorexia group. There were 14 taxa in the anorexia group and 11 taxa in the healthy control group at the phylum level, and 193 taxa in the anorexia group and 180 in the control group at the genus level. The dominant bacteria at the phylum level of the two groups were the same, while there were 16 dominant bacteria taxa in the anorexia group and 17 in the control group at the genus level. The ratio of percentage abundance of Bacteroidetes to that of Firmicutes (the B/F index) in the anorexia group was higher than in the control group. The abundance of Bacteroidetes in the anorexia group was higher than that in the control group, and the abundance of Actinomycetes in the control group was higher than that in the anorexia group. There were significant differences in 14 dominant genera between the two groups at the genus classification level. The LEfSe multilevel species difference analysis showed that at the phylum level, the significant influential bacterial taxa in the anorexia group were Bacteroidetes and Actinobacteria in the control group. At the genus level, the significant influential bacterial taxa in the anorexia group were Bacteroides, Faecalibacterium, and Subdoligranulum, and Bifidobacterium, Blautia, Streptococcus, Lachnoclostridium, and Erysipelatoclostridium in the control group. We conclude that the increase in Bacteroides abundance or in the B/F index and the reduction in Bifidobacterium abundance were related to the pathogenesis of anorexia.

儿童非器质性厌食症的发病机制尚不清楚。本研究拟对肠道菌群进行分析，为临床合理选择微生态制剂提供相关理论依据。本研究以非器质性厌食症患儿为厌食症组，正常健康患儿为对照组。收集粪便标本，经PCR和高通量测序进行生物信息学分析。结果显示，厌食症组的Ace、Chao、Shannon指数均高于对照组，对照组的Simpson指数低于厌食症组。在门水平上厌食症组有14个类群，健康对照组有11个类群；在属水平上厌食症组有193个类群，健康对照组有180个类群。两组在门水平的优势菌群相同，而厌食症组在属水平上有16个优势菌群，对照组有17个优势菌群。厌食症组拟杆菌门（Bacteroidetes）与厚壁菌门（Firmicutes）的百分比丰度比（B/F指数）高于对照组。厌食症组拟杆菌门（Bacteroidetes）丰度高于对照组，放线菌门（actinomytes）丰度高于厌食症组。在属分类水平上，两组间有14个优势属存在显著差异。LEfSe多水平物种差异分析显示，在门水平上，厌食症组影响显著的细菌类群是对照组的拟杆菌门（Bacteroidetes）和放线菌门（Actinobacteria）。在属水平上，厌食症组影响显著的细菌类群为拟杆菌（Bacteroides）、粪杆菌（Faecalibacterium）和亚doligranulum，对照组影响显著的细菌类群为双歧杆菌（Bifidobacterium）、蓝杆菌（Blautia）、链球菌（Streptococcus）、Lachnoclostridium和丹毒杆菌（Erysipelatoclostridium）。我们认为，拟杆菌丰度或B/F指数的增加和双歧杆菌丰度的减少与厌食症的发病机制有关。

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引用次数: 0

Genetic resistance to infectious diseases: A complementary tool to antimicrobial drugs in livestock? 对传染病的遗传抗性：家畜抗微生物药物的补充工具？

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-07-07 DOI: 10.1016/j.ram.2025.06.001

Carlos A. Rossetti , Ursula A. Rossi

引用次数: 0

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