Revista Argentina de microbiologia最新文献

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Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification 从牛乳腺炎中分离出的链球菌：抗菌药敏感性研究以及常规表型诊断与分子鉴定之间的分歧评估。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.07.006

Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes

Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. Streptococcus bacteria, particularly Streptococcus uberis, Streptococcus agalactiae, and Streptococcus dysgalactiae, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 Streptococcus strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated S. dysgalactiae as the most prevalent (44.35%), followed by S. uberis (24.34%) and S. agalactiae (6.09%). However, the genotypic identification revealed S. uberis as the most prevalent, followed by S. dysgalactiae, while S. agalactiae remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by Streptococcus in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for Streptococcus species responsible for bovine mastitis.

牛乳腺炎对全球乳制品生产构成重大威胁，导致牛奶产量的大量损失。链球菌，尤其是尤伯杯链球菌、无乳链球菌和半乳链球菌通常与这种疾病有关。准确的诊断对于实施有效治疗和尽量减少对生产的影响至关重要。本研究利用聚合酶链式反应（PCR）技术对从乌拉圭牛乳腺炎病例中分离出的 115 株链球菌进行了菌种鉴定。此外，研究还评估了其中 81 株细菌对四环素、红霉素和青霉素的耐药性。在所有三个物种中，表型检测和基因型检测之间存在明显差异，只有 31% 的菌株表型鉴定结果与 PCR 结果一致。表型流行率显示，痢疾杆菌最流行（44.35%），其次是尤伯杯痢疾杆菌（24.34%）和赤乳痢疾杆菌（6.09%）。然而，基因型鉴定结果显示，最常见的是 S. uberis，其次是 S. dysgalactiae，而 S. agalactiae 的流行率最低。聚合酶链式反应的高灵敏度和快速性表明，任何实验室都有可能将其作为诊断链球菌引起的牛乳腺炎的常规方法。虽然青霉素耐药性几乎不存在，但四环素和红霉素在所研究的三个物种中都表现出较高的耐药性。总之，该研究强调了早期诊断的重要性，突出了细菌流行率的变化，并建议将 PCR 作为诊断牛乳腺炎链球菌的重要工具。

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引用次数: 0

Norovirus outbreaks in a nursery school in Buenos Aires, Argentina 阿根廷布宜诺斯艾利斯一家幼儿园爆发诺如病毒。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.07.001

Karina A. Gomes, Juan I. Degiuseppe, Juan A. Stupka

Norovirus (NoV) is the leading cause of outbreaks of acute gastroenteritis worldwide. These are non-enveloped viruses that are classified into 10 genogroups, of which genogroup I (GI), II (GII), IV (GIV), VIII (GVIII), and IX (GIX) are the ones that infect humans. Two outbreaks (A and B) of acute gastroenteritis that occurred in a nursery school are described. The first outbreak (A) occurred in November 2018, and the second (B) in February 2020. The detection of viral and bacterial pathogens was performed to study both outbreaks. Additionally, an epidemiological investigation of the outbreaks was conducted. In the analyzed fecal and vomit samples from both children and adults in the nursery school, NoV GII.4 [P16] Sydney 2012 and NoV GI.3 [P13] were detected in outbreaks A and B, respectively. Since the study of acute gastroenteritis outbreaks is underestimated in Argentina, it is necessary to design prevention, study, and control protocols, as well as to improve the outbreak notification system in our country.

诺如病毒（NoV）是全球爆发急性肠胃炎的主要原因。诺如病毒是一种非显性病毒，分为 10 个基因组，其中基因组 I (GI)、II (GII)、IV (GIV)、VIII (GVIII) 和 IX (GIX) 是感染人类的病毒。本文描述了在一所幼儿园爆发的两起急性肠胃炎（A 和 B）。第一次爆发（A）发生在 2018 年 11 月，第二次爆发（B）发生在 2020 年 2 月。为研究这两起疫情，对病毒和细菌病原体进行了检测。此外，还对疫情进行了流行病学调查。在分析的幼儿园儿童和成人的粪便和呕吐物样本中，分别检测到 A 次和 B 次疫情中的 NoV GII.4 [P16] Sydney 2012 和 NoV GI.3 [P13]。由于阿根廷对急性肠胃炎疫情的研究估计不足，因此有必要制定预防、研究和控制方案，并改进我国的疫情通报系统。

引用次数: 0

Cutaneous infection in an immunocompromised domestic cat caused by Setosphaeria turcica (Luttr.) Leonard & Suggs 由Setosphaeria turcica (Luttr.) Leonard & Suggs引起的一只免疫力低下家猫的皮肤感染。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.08.003

Fábio Antônio Antonelo , Mariana Soares Rodrigues , Thiago Jean Kraus , Mayara Souza da Silva , Paula Fernandes Montanher

This is the first report of a cutaneous infection in an immunocompromised domestic cat caused by Setosphaeria turcica. The investigation encompasses an assessment of its virulence factors and susceptibility to antifungal drugs. The isolated strain originated from a domestic cat displaying cutaneous lesions that tested positive for feline leukemia virus (FeLV) infection. Identification procedures employed both microscopic and molecular techniques, with molecular identification relying on ITS DNA sequencing. Enzymatic assays targeting lipase, phospholipase, protease, and keratinase yielded negative results, suggesting the prevalence of alternative virulence mechanisms. Successful treatment of the infection was achieved with itraconazole, and susceptibility testing confirmed its sensitivity to azoles and polyene antifungal drugs.

这是首次报道免疫力低下的家猫受到土耳其节肢动物真菌（Setosphaeria turcica）的皮肤感染。调查包括对其致病因素和对抗真菌药物敏感性的评估。分离出的菌株来源于一只家猫，它的皮肤病变对猫白血病病毒（FeLV）感染呈阳性反应。鉴定程序采用了显微镜和分子技术，其中分子鉴定依赖于 ITS DNA 测序。针对脂肪酶、磷脂酶、蛋白酶和角蛋白酶的酶学检测结果均为阴性，表明存在其他致病机制。伊曲康唑成功治疗了该感染，药敏试验证实了它对唑类和多烯类抗真菌药物的敏感性。

引用次数: 0

Chagas disease and immunosuppressive therapy: When therapeutic interventions can harm 恰加斯病和免疫抑制疗法：当治疗干预可能有害。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.11.004

Catalina D. Alba Soto , Silvia A. Repetto

引用次数: 0

Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes 农业土壤中的厌氧消化物：系统回顾其对抗生素抗性基因的影响。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.07.005

Marco Allegrini , María Celina Zabaloy

Tackling the dissemination of antibiotic resistance is one of the main global challenges. Manures from animal production are a recognized source of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) requiring appropriate treatment methods. One of the main approaches for manure treatment is anaerobic digestion (AD). Meta-analyses have demonstrated that AD can significantly reduce the load of ARGs. However, antibiotics, ARGs and MGEs still remain in the final product (digestate). A sustainable agricultural use of digestates under the One Health framework requires wide assessments of their effects in the soil resistome. The objective of this review was to present the state of the art of digestate effects on ARGs of agricultural soils, focusing exclusively on digestates from animal manures. A systematic review was conducted. The examination of the resulting literature indicated that although temporal decays are observed for a variety of ARGs in single-application and repeated-applications experiments, for certain ARGs the pre-treatment or control levels are not restored. However, the low number of studies and the heterogeneous experimental conditions preclude a clear understanding of the fate of ARGs in soil and their risk for agroecosystems. The inclusion of multiple MGEs and the assessment of the long-term influence of digestates on soil properties and microbial communities could be keystones for a better understanding of the risks associated with digestate-induced changes in the soil resistome.

应对抗生素耐药性的传播是全球面临的主要挑战之一。动物生产产生的粪便是公认的抗生素耐药性基因（ARGs）和移动遗传因子（MGEs）的来源，需要采用适当的处理方法。厌氧消化（AD）是粪便处理的主要方法之一。元分析表明，厌氧消化可显著减少 ARGs 的负荷。然而，抗生素、ARGs 和 MGEs 仍会残留在最终产品（沼渣）中。要在 "同一健康 "框架下实现沼渣的可持续农业利用，就必须对其在土壤抗性组中的影响进行广泛评估。本综述的目的是介绍沼渣对农业土壤ARGs影响的最新进展，重点关注来自动物粪便的沼渣。我们进行了系统性的回顾。研究结果表明，虽然在单次施用和重复施用实验中观察到了各种 ARGs 的时间衰减，但某些 ARGs 无法恢复到处理前或对照组的水平。然而，由于研究数量少，实验条件不尽相同，因此无法清楚地了解 ARGs 在土壤中的归宿及其对农业生态系统的风险。纳入多个多边环境协定以及评估沼渣对土壤特性和微生物群落的长期影响，是更好地了解沼渣引起的土壤抗性组变化的相关风险的关键。

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引用次数: 0

Investigación del perfil de resistencia a betalactámicos en cepas clínicas de Pseudomonas aeruginosa en Mexicali, 2019-2021 [调查墨西卡利铜绿假单胞菌菌株对 Beta-内酰胺类药物的耐药性概况：2019-2021 年]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.10.002

Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa

Pseudomonas aeruginosa is a Gram-negative bacillus capable of developing in humid environments and animal tissue. The interest in this bacterium lies in its ability to cause opportunistic diseases in patients with cystic fibrosis and healthcare-associated infections (HAIs). The objective of our study was to characterize the resistance profile of strains causing HAIs isolated in hospitals within our community, from January 2019 to December 2021. This descriptive, prospective, and cross-sectional study involved the isolation of strains from January 2019 to December 2021 at the Autonomous University of Baja California (UABC). The identification of the strains was carried out using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry, and the detection of beta-lactam resistance was performed according to the criteria of the Clinical and Laboratory Standards Institute as stipulated in the CLSI M100-S27 document. A total of 649 samples were obtained from January 2019 to December 2021, including sputum (335 samples), urine (119 samples), and wounds (91 samples). Resistance to carbapenems was 38.94% for meropenem and 21.97% for imipenem. For cephalosporins, there was a 21.05% resistance rate for cefepime, 22.9% for ceftazidime, and 24.78% for ceftolozane-tazobactam. The prevalence of antimicrobial resistance has increased over time, which is attributable to both selective pressure and the evolution of the microorganisms themselves.

铜绿假单胞菌是一种革兰氏阴性杆菌，能够在潮湿环境和动物组织中生长。人们对这种细菌的兴趣在于它能引起囊性纤维化患者的机会性疾病和医疗相关感染（HAIs）。我们的研究目的是分析 2019 年 1 月至 2021 年 12 月期间在社区内的医院中分离到的导致 HAIs 的菌株的耐药性特征。这项描述性、前瞻性和横断面研究涉及从 2019 年 1 月至 2021 年 12 月在下加利福尼亚自治大学（UABC）分离的菌株。采用基质辅助激光解吸/电离-飞行时间（MALDI-TOF）质谱法对菌株进行鉴定，并根据 CLSI M100-S27 文件中规定的临床和实验室标准研究所的标准对β-内酰胺耐药性进行检测。从2019年1月至2021年12月，共采集了649份样本，包括痰（335份样本）、尿（119份样本）和伤口（91份样本）。美罗培南对碳青霉烯类的耐药率为 38.94%，亚胺培南为 21.97%。头孢菌素类药物中，头孢吡肟的耐药率为 21.05%，头孢他啶为 22.9%，头孢唑烷-他唑巴坦为 24.78%。随着时间的推移，抗菌素耐药性的发生率也在增加，这既是选择性压力造成的，也是微生物自身进化的结果。

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引用次数: 0

Incidencia de microorganismos deteriorantes de la calidad de cerveza en microcervecerías de Buenos Aires [布宜诺斯艾利斯微型啤酒厂啤酒腐败微生物的发生率]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-10-01 DOI: 10.1016/j.ram.2024.09.006

Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar

Microbial contaminations pose a significant concern within the brewing industry, exerting negative effects on the organoleptic quality of the product and leading to substantial economic losses. The exponential proliferation of craft breweries in Argentina in recent years has heightened the demand for constant improvements in processes to ensure excellence in beer production. However, the stringency of microbiological quality controls remains a vulnerable area. This study assesses the prevalence of beer contaminants in samples from 10 breweries located in Buenos Aires City (CABA) and Greater Buenos Aires area (GBA). The results revealed the presence of microorganisms in 70% of the analyzed samples. Fifteen bacteria and 19 yeasts were successfully isolated, with bacteria belonging to the genera Acetobacter and Staphylococcus, and yeasts to the genera Saccharomyces, Lodderomyces, Candida, and Pichia. Accurately identifying these microorganisms provides producers with the necessary information for formulating action plans to improve cleaning and sanitization protocols in their facilities. This proactive approach not only has the potential to mitigate economic losses associated with microbial contamination but also contributes to maintaining and elevating quality standards in regional craft beer production.

微生物污染是酿造业的一个重大问题，会对产品的感官质量造成负面影响，并导致巨大的经济损失。近年来，阿根廷手工酿造啤酒的数量激增，这就要求不断改进工艺，以确保啤酒生产的卓越品质。然而，微生物质量控制的严格程度仍然是一个薄弱环节。本研究评估了布宜诺斯艾利斯市（CABA）和大布宜诺斯艾利斯地区（GBA）10 家啤酒厂样品中啤酒污染物的流行情况。结果显示，70% 的分析样品中都含有微生物。成功分离出 15 种细菌和 19 种酵母菌，其中细菌属于醋酸杆菌属和葡萄球菌属，酵母菌属于酵母菌属、菟丝子属、念珠菌属和毕赤菌属。准确识别这些微生物可为生产商提供必要的信息，帮助他们制定行动计划，改进设备的清洁和消毒规程。这种积极主动的方法不仅有可能减轻微生物污染带来的经济损失，还有助于保持和提高地区精酿啤酒生产的质量标准。

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引用次数: 0

Prokaryotes versus Eukaryotes: Which is the host and which is the guest? 原核生物与真核生物：哪个是主人，哪个是客人？

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.09.001

Guillermo Tellez-Isaias , Dante Javier Bueno

引用次数: 0

Caracterización de una comunidad microbiana aislada de colonias de abejas melíferas [从蜜蜂群中分离出来的微生物群落的特征]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.01.001

The microbial communities within honey bee colonies contribute to the defense against pathogens. The goal of this study was to isolate, identify, and lyophilize lactic acid bacteria and bifidobacteria from the gut of nurse bees and bee bread in Apis mellifera colonies. Bacterial cultures from the intestinal content were conducted, and subsequently identified, sequenced, and lyophilized. Cross-antagonism among them was also assessed. Studies based on 16 S rRNA gene Sanger sequencing revealed that the MC3 strain had 100% identity with Bifidobacterium choladohabitans, the PP2B strain showed 99.16% similarity with Enterococcus faecium, while the PP1 strain exhibited 99.49% similarity with Lacticaseibacillus sp. and the PP1B strain showed 99.32% similarity with Lacticaseibacillus sp. There was no evidence of cross-antagonism among the strains, and the lyophilization process showed good stability and conservation. This is the first report of the isolation of B. choladohabitans from honey bee gut in Argentina, and also associates the presence of E. faecium with bee bread.

蜜蜂群落中的微生物群落有助于抵御病原体。本研究的目的是从蜜蜂群中的哺育蜂和蜜蜂面包的肠道中分离、鉴定和冻干乳酸菌和双歧杆菌。从肠道内容物中进行了细菌培养，随后进行了鉴定、测序和冻干。还对它们之间的交叉拮抗作用进行了评估。基于 16 S rRNA 基因 Sanger 测序的研究表明，MC3 菌株与胆酸双歧杆菌的相似度为 100%，PP2B 菌株与粪肠球菌的相似度为 99.16%，而 PP1 菌株与乳酸杆菌的相似度为 99.49%。菌株之间没有交叉拮抗的迹象，冻干过程显示出良好的稳定性和保存性。这是阿根廷首次从蜜蜂肠道中分离出B. Choladohabitans的报告，同时也将粪肠球菌与蜜蜂面包联系起来。

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引用次数: 0

Los fructanos de agave promueven la formación de biopelícula in vitro en el consorcio probiótico Lactobacillus delbrueckii ssp. lactis, L. delbrueckii ssp. bulgaricus y Streptococcus thermophilus [龙舌兰果糖促进益生菌联合体--德尔布鲁贝克乳杆菌、保加利亚乳杆菌和嗜热链球菌--体外生物膜的形成]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2024-07-01 DOI: 10.1016/j.ram.2024.02.002

In recent years the relationship between the intestinal microbiota, the host and chronic non-communicable diseases has brought interest into the study of its formation and maintenance in the host. Lactic acid bacteria (BAL) are Gram-positive bacteria with probiotic activity, which have been associated with many health benefits, such as decreased body fat mass and lower risk of type II diabetes mellitus. One of the main colonization mechanisms and bacteria survival strategies is the production of biofilms and the use of prebiotics as substrates to achieve a balance within intestinal microbiota. However, there is not enough evidence to demonstrate the biofilm formation in the presence of agave fructans (AF). This study aimed to evaluate in vitro the biofilm formation in a consortium of lactic acid bacteria: Lactobacillus delbrueckii ssp. lactis, Lactobacillus delbrueckii ssp. bulgaricus y Streptococcus thermophilus in the presence of AF at different concentrations: 0%, 0,1%, 4%, 8% y 16%. The addition of 0,1% of AF correlates with the best capacity for biofilm formation. The findings imply the possibility of modulating the biofilm formation of lactic acid bacteria with AF. These results can contribute positively to the host, by generating intestinal homeostasis, colonization resistance, stability to food digestion and chemical modifications of drugs and carry out beneficial functions to the health.

近年来，肠道微生物群、宿主和慢性非传染性疾病之间的关系引起了人们对其在宿主体内形成和维持的研究兴趣。乳酸菌（BAL）是具有益生菌活性的革兰氏阳性细菌，与许多健康益处有关，如减少体脂量和降低 II 型糖尿病风险。主要的定植机制和细菌生存策略之一是产生生物膜和使用益生元作为底物，以实现肠道微生物群的平衡。然而，目前还没有足够的证据证明龙舌兰果聚糖（AF）会形成生物膜。本研究旨在体外评估乳酸菌群的生物膜形成情况：乳酸杆菌、保加利亚乳杆菌和嗜热链球菌在不同浓度的龙舌兰果糖存在下的生物膜形成情况：0%, 0,1%, 4%, 8% y 16%.添加 0.1% AF 的生物膜形成能力最强。研究结果表明，添加 AF 有可能调节乳酸菌生物膜的形成。这些结果可通过产生肠道平衡、抗定植性、食物消化稳定性和药物化学修饰，对宿主做出积极贡献，并对健康产生有益的作用。

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引用次数: 0

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