Pub Date : 2024-10-01Epub Date: 2024-10-02DOI: 10.1016/j.ram.2024.08.001
Ana C. Gomez-Yanes , Karina D. Garcia-Orozco , Veronica Vazquez-Villarce , Elena N. Moreno-Cordova , Veronica Mata-Haro , Marcia Leyva-Gastelum , Adan Valenzuela-Castillo , Alonso A. Lopez-Zavala , Ana M. Calderon de la Barca , Jesus G. Valenzuela , Rogerio R. Sotelo-Mundo
The brown dog tick (Rhipicephalus sanguineus) is the vector of Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever (RMSF) in Northern Mexico and Southwestern United States. The immune response to a tick protein in the sera of humans or animals may reveal the zones with a high propensity to acquire RMSF, and vector control strategies may be focused on these zones. Arginine kinase (AK) is a highly antigenic invertebrate protein that may serve as a marker for tick exposure. We used R. sanguineus recombinant AK in an indirect ELISA assay with RMSF-positive patient sera. The response to AK was significantly higher against the sera of RMSF patients than the control sera from healthy participants without contact with dogs. To validate the antigenicity of tick AK, we mutated one predicted conformational epitope to alanine residues, which reduced the recognition by RMSF patients’ immunoglobulins. This preliminary result opens a perspective towards the development of a complimentary technique based on RsAK as an antigen biomarker for vector serological surveillance for Rickettsia RMSF prevention.
棕狗蜱（Rhipicephalus sanguineus）是落基山斑疹热（RMSF）病原体立克次体在墨西哥北部和美国西南部的传播媒介。人或动物血清中对蜱蛋白的免疫反应可能会揭示洛矶山斑疹热的高发区，病媒控制策略可能会侧重于这些区域。精氨酸激酶（AK）是一种高抗原性的无脊椎动物蛋白，可作为蜱接触的标记物。我们使用 R. sanguineus 重组 AK 与 RMSF 阳性患者血清进行间接 ELISA 检测。RMSF 患者血清对 AK 的反应明显高于未接触过狗的健康参与者的对照血清。为了验证蜱虫 AK 的抗原性，我们将一个预测的构象表位突变为丙氨酸残基，从而降低了 RMSF 患者免疫球蛋白的识别率。这一初步结果为开发基于RsAK的辅助技术开辟了前景，RsAK是一种抗原生物标记物，可用于预防立克次体RMSF的病媒血清学监测。
{"title":"The potential use of arginine kinase from the brown tick Rhipicephalus sanguineus as a biomarker for vector exposure in the surveillance of Rocky Mountain spotted fever","authors":"Ana C. Gomez-Yanes , Karina D. Garcia-Orozco , Veronica Vazquez-Villarce , Elena N. Moreno-Cordova , Veronica Mata-Haro , Marcia Leyva-Gastelum , Adan Valenzuela-Castillo , Alonso A. Lopez-Zavala , Ana M. Calderon de la Barca , Jesus G. Valenzuela , Rogerio R. Sotelo-Mundo","doi":"10.1016/j.ram.2024.08.001","DOIUrl":"10.1016/j.ram.2024.08.001","url":null,"abstract":"<div><div>The brown dog tick (<em>Rhipicephalus sanguineus</em>) is the vector of <em>Rickettsia rickettsii</em>, the causative agent of Rocky Mountain spotted fever (RMSF) in Northern Mexico and Southwestern United States. The immune response to a tick protein in the sera of humans or animals may reveal the zones with a high propensity to acquire RMSF, and vector control strategies may be focused on these zones. Arginine kinase (AK) is a highly antigenic invertebrate protein that may serve as a marker for tick exposure. We used <em>R. sanguineus</em> recombinant AK in an indirect ELISA assay with RMSF-positive patient sera. The response to AK was significantly higher against the sera of RMSF patients than the control sera from healthy participants without contact with dogs. To validate the antigenicity of tick AK, we mutated one predicted conformational epitope to alanine residues, which reduced the recognition by RMSF patients’ immunoglobulins. This preliminary result opens a perspective towards the development of a complimentary technique based on <em>Rs</em>AK as an antigen biomarker for vector serological surveillance for <em>Rickettsia</em> RMSF prevention.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 364-367"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-06-27DOI: 10.1016/j.ram.2023.12.007
Nohemí Catana-Botello , Pola Becerril-Montes , Jorge Castro-Garza , Francisco González-Salazar , Horacio Almanza-Reyes , María De Los Ángeles Del Bosque-Moncayo , Alejandro Morales-Vargas , Víctor Manuel Velázquez-Moreno
Tuberculosis remains a serious threat to human health as an infectious disease in Mexico. Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in the State of Nuevo Leon, Mexico are scarce. We aimed to determine the genotypes of circulating MTB belonging to the Beijing lineage recovered from patients in the State of Nuevo Leon, Mexico. A total of 406 MTB isolates from this state were genotyped using the spoligotyping method and 18-locus MIRU-VNTR. Lineage classification and MTB transmission analysis were performed. Based on the spoligotyping analysis, we found 24 strains belonging to the Beijing genotype that were characterized phylogenetically. The MIRUs showed greater discriminatory power than the standard RFLP-IS6110 method; therefore, the greatest allelic diversity among the Beijing strains was observed with MIRU10, MIRU31, MIRU39, MRU40, and MIRU 26. MVLA analysis showed a profile variation between Beijing and non-Beijing strains. The minimum spanning tree (MST) showed that 79% (19) of the strains are related. All Beijing strains exhibited the deletion of region TbD1, which is a characteristic of modern strains. The application of spoligotyping and MIRU-VNTR-18 methods together proved to be more sensitive, discriminatory, and rapid than the standard method for the epidemiological analysis of Mycobacterium Beijing isolates. This study is one of the first to describe the genomic diversity of M. Beijing in the State of Nuevo Leon, Mexico.
{"title":"Mycobacterium tuberculosis Beijing in the State of Nuevo Leon, Mexico","authors":"Nohemí Catana-Botello , Pola Becerril-Montes , Jorge Castro-Garza , Francisco González-Salazar , Horacio Almanza-Reyes , María De Los Ángeles Del Bosque-Moncayo , Alejandro Morales-Vargas , Víctor Manuel Velázquez-Moreno","doi":"10.1016/j.ram.2023.12.007","DOIUrl":"10.1016/j.ram.2023.12.007","url":null,"abstract":"<div><div>Tuberculosis remains a serious threat to human health as an infectious disease in Mexico. Data about the genotypes of circulating <em>Mycobacterium tuberculosis</em> isolates (MTB) in the State of Nuevo Leon, Mexico are scarce. We aimed to determine the genotypes of circulating MTB belonging to the Beijing lineage recovered from patients in the State of Nuevo Leon, Mexico. A total of 406 MTB isolates from this state were genotyped using the spoligotyping method and 18-locus MIRU-VNTR. Lineage classification and MTB transmission analysis were performed. Based on the spoligotyping analysis, we found 24 strains belonging to the Beijing genotype that were characterized phylogenetically. The MIRUs showed greater discriminatory power than the standard RFLP-IS6110 method; therefore, the greatest allelic diversity among the Beijing strains was observed with MIRU10, MIRU31, MIRU39, MRU40, and MIRU 26. MVLA analysis showed a profile variation between Beijing and non-Beijing strains. The minimum spanning tree (MST) showed that 79% (19) of the strains are related. All Beijing strains exhibited the deletion of region TbD1, which is a characteristic of modern strains. The application of spoligotyping and MIRU-VNTR-18 methods together proved to be more sensitive, discriminatory, and rapid than the standard method for the epidemiological analysis of <em>Mycobacterium Beijing</em> isolates. This study is one of the first to describe the genomic diversity of <em>M. Beijing</em> in the State of Nuevo Leon, Mexico.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 380-389"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-09-02DOI: 10.1016/j.ram.2024.07.001
Karina A. Gomes, Juan I. Degiuseppe, Juan A. Stupka
Norovirus (NoV) is the leading cause of outbreaks of acute gastroenteritis worldwide. These are non-enveloped viruses that are classified into 10 genogroups, of which genogroup I (GI), II (GII), IV (GIV), VIII (GVIII), and IX (GIX) are the ones that infect humans. Two outbreaks (A and B) of acute gastroenteritis that occurred in a nursery school are described. The first outbreak (A) occurred in November 2018, and the second (B) in February 2020. The detection of viral and bacterial pathogens was performed to study both outbreaks. Additionally, an epidemiological investigation of the outbreaks was conducted. In the analyzed fecal and vomit samples from both children and adults in the nursery school, NoV GII.4 [P16] Sydney 2012 and NoV GI.3 [P13] were detected in outbreaks A and B, respectively. Since the study of acute gastroenteritis outbreaks is underestimated in Argentina, it is necessary to design prevention, study, and control protocols, as well as to improve the outbreak notification system in our country.
诺如病毒（NoV）是全球爆发急性肠胃炎的主要原因。诺如病毒是一种非显性病毒，分为 10 个基因组，其中基因组 I (GI)、II (GII)、IV (GIV)、VIII (GVIII) 和 IX (GIX) 是感染人类的病毒。本文描述了在一所幼儿园爆发的两起急性肠胃炎（A 和 B）。第一次爆发（A）发生在 2018 年 11 月，第二次爆发（B）发生在 2020 年 2 月。为研究这两起疫情，对病毒和细菌病原体进行了检测。此外，还对疫情进行了流行病学调查。在分析的幼儿园儿童和成人的粪便和呕吐物样本中，分别检测到 A 次和 B 次疫情中的 NoV GII.4 [P16] Sydney 2012 和 NoV GI.3 [P13]。由于阿根廷对急性肠胃炎疫情的研究估计不足，因此有必要制定预防、研究和控制方案，并改进我国的疫情通报系统。
{"title":"Norovirus outbreaks in a nursery school in Buenos Aires, Argentina","authors":"Karina A. Gomes, Juan I. Degiuseppe, Juan A. Stupka","doi":"10.1016/j.ram.2024.07.001","DOIUrl":"10.1016/j.ram.2024.07.001","url":null,"abstract":"<div><div>Norovirus (NoV) is the leading cause of outbreaks of acute gastroenteritis worldwide. These are non-enveloped viruses that are classified into 10 genogroups, of which genogroup I (GI), II (GII), IV (GIV), VIII (GVIII), and IX (GIX) are the ones that infect humans. Two outbreaks (A and B) of acute gastroenteritis that occurred in a nursery school are described. The first outbreak (A) occurred in November 2018, and the second (B) in February 2020. The detection of viral and bacterial pathogens was performed to study both outbreaks. Additionally, an epidemiological investigation of the outbreaks was conducted. In the analyzed fecal and vomit samples from both children and adults in the nursery school, NoV GII.4 [P16] Sydney 2012 and NoV GI.3 [P13] were detected in outbreaks A and B, respectively. Since the study of acute gastroenteritis outbreaks is underestimated in Argentina, it is necessary to design prevention, study, and control protocols, as well as to improve the outbreak notification system in our country.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 373-379"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-09-19DOI: 10.1016/j.ram.2024.07.006
Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes
Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. Streptococcus bacteria, particularly Streptococcus uberis, Streptococcus agalactiae, and Streptococcus dysgalactiae, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 Streptococcus strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated S. dysgalactiae as the most prevalent (44.35%), followed by S. uberis (24.34%) and S. agalactiae (6.09%). However, the genotypic identification revealed S. uberis as the most prevalent, followed by S. dysgalactiae, while S. agalactiae remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by Streptococcus in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for Streptococcus species responsible for bovine mastitis.
牛乳腺炎对全球乳制品生产构成重大威胁，导致牛奶产量的大量损失。链球菌，尤其是尤伯杯链球菌、无乳链球菌和半乳链球菌通常与这种疾病有关。准确的诊断对于实施有效治疗和尽量减少对生产的影响至关重要。本研究利用聚合酶链式反应（PCR）技术对从乌拉圭牛乳腺炎病例中分离出的 115 株链球菌进行了菌种鉴定。此外，研究还评估了其中 81 株细菌对四环素、红霉素和青霉素的耐药性。在所有三个物种中，表型检测和基因型检测之间存在明显差异，只有 31% 的菌株表型鉴定结果与 PCR 结果一致。表型流行率显示，痢疾杆菌最流行（44.35%），其次是尤伯杯痢疾杆菌（24.34%）和赤乳痢疾杆菌（6.09%）。然而，基因型鉴定结果显示，最常见的是 S. uberis，其次是 S. dysgalactiae，而 S. agalactiae 的流行率最低。聚合酶链式反应的高灵敏度和快速性表明，任何实验室都有可能将其作为诊断链球菌引起的牛乳腺炎的常规方法。虽然青霉素耐药性几乎不存在，但四环素和红霉素在所研究的三个物种中都表现出较高的耐药性。总之，该研究强调了早期诊断的重要性，突出了细菌流行率的变化，并建议将 PCR 作为诊断牛乳腺炎链球菌的重要工具。
{"title":"Streptococcus spp. isolated from bovine mastitis: Antimicrobial sensitivity studies and disagreement evaluation between routine phenotypic diagnosis and molecular identification","authors":"Leticia Diana , Lucas Mastroianni , Virginia Diana , Rodrigo Puentes","doi":"10.1016/j.ram.2024.07.006","DOIUrl":"10.1016/j.ram.2024.07.006","url":null,"abstract":"<div><div>Bovine mastitis poses a significant threat to global dairy production, resulting in substantial losses in milk production. <em>Streptococcus</em> bacteria, particularly <em>Streptococcus uberis</em>, <em>Streptococcus agalactiae</em>, and <em>Streptococcus dysgalactiae</em>, are commonly implicated in this condition. An accurate diagnosis is crucial for implementing effective treatment and minimizing its impact on production. This study examined 115 <em>Streptococcus</em> strains isolated from bovine mastitis cases in Uruguay using PCR for species identification. Additionally, the resistance to tetracycline, erythromycin, and penicillin was assessed in 81 of the bacterial strains under study. Significant disparities between phenotypic and genotypic detection were evident across all three species, with only 31% of strains identified phenotypically aligning with PCR results. Phenotypic prevalence indicated <em>S. dysgalactiae</em> as the most prevalent (44.35%), followed by <em>S. uberis</em> (24.34%) and <em>S. agalactiae</em> (6.09%). However, the genotypic identification revealed <em>S. uberis</em> as the most prevalent, followed by <em>S. dysgalactiae</em>, while <em>S. agalactiae</em> remained the least prevalent. The high sensitivity and speed of PCR suggest its potential routine implementation for diagnosing bovine mastitis caused by <em>Streptococcus</em> in any laboratory. Although, penicillin resistance was practically nonexistent, tetracycline and erythromycin exhibit higher resistance levels across all three species studied. In conclusion, the study underlines the importance of early diagnosis, highlights variations in bacterial prevalence, and proposes PCR as a valuable diagnostic tool for <em>Streptococcus</em> species responsible for bovine mastitis.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 351-358"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-11-12DOI: 10.1016/j.ram.2024.08.003
Fábio Antônio Antonelo , Mariana Soares Rodrigues , Thiago Jean Kraus , Mayara Souza da Silva , Paula Fernandes Montanher
This is the first report of a cutaneous infection in an immunocompromised domestic cat caused by Setosphaeria turcica. The investigation encompasses an assessment of its virulence factors and susceptibility to antifungal drugs. The isolated strain originated from a domestic cat displaying cutaneous lesions that tested positive for feline leukemia virus (FeLV) infection. Identification procedures employed both microscopic and molecular techniques, with molecular identification relying on ITS DNA sequencing. Enzymatic assays targeting lipase, phospholipase, protease, and keratinase yielded negative results, suggesting the prevalence of alternative virulence mechanisms. Successful treatment of the infection was achieved with itraconazole, and susceptibility testing confirmed its sensitivity to azoles and polyene antifungal drugs.
这是首次报道免疫力低下的家猫受到土耳其节肢动物真菌（Setosphaeria turcica）的皮肤感染。调查包括对其致病因素和对抗真菌药物敏感性的评估。分离出的菌株来源于一只家猫，它的皮肤病变对猫白血病病毒（FeLV）感染呈阳性反应。鉴定程序采用了显微镜和分子技术，其中分子鉴定依赖于 ITS DNA 测序。针对脂肪酶、磷脂酶、蛋白酶和角蛋白酶的酶学检测结果均为阴性，表明存在其他致病机制。伊曲康唑成功治疗了该感染，药敏试验证实了它对唑类和多烯类抗真菌药物的敏感性。
{"title":"Cutaneous infection in an immunocompromised domestic cat caused by Setosphaeria turcica (Luttr.) Leonard & Suggs","authors":"Fábio Antônio Antonelo , Mariana Soares Rodrigues , Thiago Jean Kraus , Mayara Souza da Silva , Paula Fernandes Montanher","doi":"10.1016/j.ram.2024.08.003","DOIUrl":"10.1016/j.ram.2024.08.003","url":null,"abstract":"<div><div>This is the first report of a cutaneous infection in an immunocompromised domestic cat caused by <em>Setosphaeria turcica</em>. The investigation encompasses an assessment of its virulence factors and susceptibility to antifungal drugs. The isolated strain originated from a domestic cat displaying cutaneous lesions that tested positive for feline leukemia virus (FeLV) infection. Identification procedures employed both microscopic and molecular techniques, with molecular identification relying on ITS DNA sequencing. Enzymatic assays targeting lipase, phospholipase, protease, and keratinase yielded negative results, suggesting the prevalence of alternative virulence mechanisms. Successful treatment of the infection was achieved with itraconazole, and susceptibility testing confirmed its sensitivity to azoles and polyene antifungal drugs.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 390-393"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-12-05DOI: 10.1016/j.ram.2024.11.004
Catalina D. Alba Soto , Silvia A. Repetto
{"title":"Chagas disease and immunosuppressive therapy: When therapeutic interventions can harm","authors":"Catalina D. Alba Soto , Silvia A. Repetto","doi":"10.1016/j.ram.2024.11.004","DOIUrl":"10.1016/j.ram.2024.11.004","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 349-350"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-09-19DOI: 10.1016/j.ram.2024.07.005
Marco Allegrini , María Celina Zabaloy
Tackling the dissemination of antibiotic resistance is one of the main global challenges. Manures from animal production are a recognized source of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) requiring appropriate treatment methods. One of the main approaches for manure treatment is anaerobic digestion (AD). Meta-analyses have demonstrated that AD can significantly reduce the load of ARGs. However, antibiotics, ARGs and MGEs still remain in the final product (digestate). A sustainable agricultural use of digestates under the One Health framework requires wide assessments of their effects in the soil resistome. The objective of this review was to present the state of the art of digestate effects on ARGs of agricultural soils, focusing exclusively on digestates from animal manures. A systematic review was conducted. The examination of the resulting literature indicated that although temporal decays are observed for a variety of ARGs in single-application and repeated-applications experiments, for certain ARGs the pre-treatment or control levels are not restored. However, the low number of studies and the heterogeneous experimental conditions preclude a clear understanding of the fate of ARGs in soil and their risk for agroecosystems. The inclusion of multiple MGEs and the assessment of the long-term influence of digestates on soil properties and microbial communities could be keystones for a better understanding of the risks associated with digestate-induced changes in the soil resistome.
{"title":"Anaerobic digestates in agricultural soils: A systematic review of their effects on antibiotic resistance genes","authors":"Marco Allegrini , María Celina Zabaloy","doi":"10.1016/j.ram.2024.07.005","DOIUrl":"10.1016/j.ram.2024.07.005","url":null,"abstract":"<div><div>Tackling the dissemination of antibiotic resistance is one of the main global challenges. Manures from animal production are a recognized source of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) requiring appropriate treatment methods. One of the main approaches for manure treatment is anaerobic digestion (AD). Meta-analyses have demonstrated that AD can significantly reduce the load of ARGs. However, antibiotics, ARGs and MGEs still remain in the final product (digestate). A sustainable agricultural use of digestates under the One Health framework requires wide assessments of their effects in the soil resistome. The objective of this review was to present the state of the art of digestate effects on ARGs of agricultural soils, focusing exclusively on digestates from animal manures. A systematic review was conducted. The examination of the resulting literature indicated that although temporal decays are observed for a variety of ARGs in single-application and repeated-applications experiments, for certain ARGs the pre-treatment or control levels are not restored. However, the low number of studies and the heterogeneous experimental conditions preclude a clear understanding of the fate of ARGs in soil and their risk for agroecosystems. The inclusion of multiple MGEs and the assessment of the long-term influence of digestates on soil properties and microbial communities could be keystones for a better understanding of the risks associated with digestate-induced changes in the soil resistome.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 394-401"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-11-20DOI: 10.1016/j.ram.2024.10.002
Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa
Pseudomonas aeruginosa is a Gram-negative bacillus capable of developing in humid environments and animal tissue. The interest in this bacterium lies in its ability to cause opportunistic diseases in patients with cystic fibrosis and healthcare-associated infections (HAIs). The objective of our study was to characterize the resistance profile of strains causing HAIs isolated in hospitals within our community, from January 2019 to December 2021. This descriptive, prospective, and cross-sectional study involved the isolation of strains from January 2019 to December 2021 at the Autonomous University of Baja California (UABC). The identification of the strains was carried out using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry, and the detection of beta-lactam resistance was performed according to the criteria of the Clinical and Laboratory Standards Institute as stipulated in the CLSI M100-S27 document. A total of 649 samples were obtained from January 2019 to December 2021, including sputum (335 samples), urine (119 samples), and wounds (91 samples). Resistance to carbapenems was 38.94% for meropenem and 21.97% for imipenem. For cephalosporins, there was a 21.05% resistance rate for cefepime, 22.9% for ceftazidime, and 24.78% for ceftolozane-tazobactam. The prevalence of antimicrobial resistance has increased over time, which is attributable to both selective pressure and the evolution of the microorganisms themselves.
{"title":"Investigación del perfil de resistencia a betalactámicos en cepas clínicas de Pseudomonas aeruginosa en Mexicali, 2019-2021","authors":"Ricardo Delgadillo-Valles , Dolores A. Marquez-Salazar , Donato A. Rechy-Iruretagoyena , Gerson N. Hernandez-Acevedo , Jonathan I. Arauz-Cabrera , Edwin Barrios-Villa","doi":"10.1016/j.ram.2024.10.002","DOIUrl":"10.1016/j.ram.2024.10.002","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a Gram-negative bacillus capable of developing in humid environments and animal tissue. The interest in this bacterium lies in its ability to cause opportunistic diseases in patients with cystic fibrosis and healthcare-associated infections (HAIs). The objective of our study was to characterize the resistance profile of strains causing HAIs isolated in hospitals within our community, from January 2019 to December 2021. This descriptive, prospective, and cross-sectional study involved the isolation of strains from January 2019 to December 2021 at the Autonomous University of Baja California (UABC). The identification of the strains was carried out using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry, and the detection of beta-lactam resistance was performed according to the criteria of the Clinical and Laboratory Standards Institute as stipulated in the CLSI M100-S27 document. A total of 649 samples were obtained from January 2019 to December 2021, including sputum (335 samples), urine (119 samples), and wounds (91 samples). Resistance to carbapenems was 38.94% for meropenem and 21.97% for imipenem. For cephalosporins, there was a 21.05% resistance rate for cefepime, 22.9% for ceftazidime, and 24.78% for ceftolozane-tazobactam. The prevalence of antimicrobial resistance has increased over time, which is attributable to both selective pressure and the evolution of the microorganisms themselves.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 368-372"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01Epub Date: 2024-11-12DOI: 10.1016/j.ram.2024.09.006
Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar
Microbial contaminations pose a significant concern within the brewing industry, exerting negative effects on the organoleptic quality of the product and leading to substantial economic losses. The exponential proliferation of craft breweries in Argentina in recent years has heightened the demand for constant improvements in processes to ensure excellence in beer production. However, the stringency of microbiological quality controls remains a vulnerable area. This study assesses the prevalence of beer contaminants in samples from 10 breweries located in Buenos Aires City (CABA) and Greater Buenos Aires area (GBA). The results revealed the presence of microorganisms in 70% of the analyzed samples. Fifteen bacteria and 19 yeasts were successfully isolated, with bacteria belonging to the genera Acetobacter and Staphylococcus, and yeasts to the genera Saccharomyces, Lodderomyces, Candida, and Pichia. Accurately identifying these microorganisms provides producers with the necessary information for formulating action plans to improve cleaning and sanitization protocols in their facilities. This proactive approach not only has the potential to mitigate economic losses associated with microbial contamination but also contributes to maintaining and elevating quality standards in regional craft beer production.
{"title":"Incidencia de microorganismos deteriorantes de la calidad de cerveza en microcervecerías de Buenos Aires","authors":"Juan I. Eizaguirre , Clara Bruzone , Ignacio Duhourq , Diego Libkind , Pablo S. Aguilar","doi":"10.1016/j.ram.2024.09.006","DOIUrl":"10.1016/j.ram.2024.09.006","url":null,"abstract":"<div><div>Microbial contaminations pose a significant concern within the brewing industry, exerting negative effects on the organoleptic quality of the product and leading to substantial economic losses. The exponential proliferation of craft breweries in Argentina in recent years has heightened the demand for constant improvements in processes to ensure excellence in beer production. However, the stringency of microbiological quality controls remains a vulnerable area. This study assesses the prevalence of beer contaminants in samples from 10 breweries located in Buenos Aires City (CABA) and Greater Buenos Aires area (GBA). The results revealed the presence of microorganisms in 70% of the analyzed samples. Fifteen bacteria and 19 yeasts were successfully isolated, with bacteria belonging to the genera <em>Acetobacter</em> and <em>Staphylococcus</em>, and yeasts to the genera <em>Saccharomyces</em>, <em>Lodderomyces</em>, <em>Candida</em>, and <em>Pichia</em>. Accurately identifying these microorganisms provides producers with the necessary information for formulating action plans to improve cleaning and sanitization protocols in their facilities. This proactive approach not only has the potential to mitigate economic losses associated with microbial contamination but also contributes to maintaining and elevating quality standards in regional craft beer production.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 4","pages":"Pages 402-412"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01Epub Date: 2024-09-16DOI: 10.1016/j.ram.2024.09.001
Guillermo Tellez-Isaias , Dante Javier Bueno
{"title":"Prokaryotes versus Eukaryotes: Which is the host and which is the guest?","authors":"Guillermo Tellez-Isaias , Dante Javier Bueno","doi":"10.1016/j.ram.2024.09.001","DOIUrl":"10.1016/j.ram.2024.09.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"56 3","pages":"Pages 201-202"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S032575412400107X/pdfft?md5=be3fafe56368886eb45684ed3b307a5b&pid=1-s2.0-S032575412400107X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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