Revista Argentina de microbiologia最新文献_第6页

Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus 突尼斯系统性红斑狼疮患者16S宏基因组学微生物多样性研究。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2025.04.002

Amira Messadi , Sameh Sayhi , Kais Ghedira , Chadia Zaouaoui , Bilel Arfaoui , Soumaya Khouikhi , Maha Rebai , Nour El Houda Guediche , Nadia Abdelhafidh , Bassem Louzir , Ajili Faida

Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case–control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus Lactobacillus (0.43% vs 0.74%), as well as a higher abundance of the genera Gammaproteobacteria (2.37% vs 0.77%) and Escherichia–Shigella (2.04% vs 0.51%) in SLE samples compared to the controls (p < 0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus Faecalibacterium (11.34% vs 3.35%) and renal manifestations (p < 0.05). The phylum Actinobacteria (0.21% vs 3.8%, p = 0.036) and the genus Bifidobacterium levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case–control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.

系统性红斑狼疮（SLE）是一种多系统自身免疫性疾病，具有显著的发病率和死亡率。它的特点是丧失自身免疫耐受性和自身抗体的产生，导致多器官损伤。新出现的研究已经证实了肠道菌群失调在SLE患者中的作用，尽管其潜在机制迄今尚不清楚。在这项研究中，我们旨在调查SLE的细菌谱，包括门/类/属的相对丰度和多样性，并将其与健康对照进行比较，并研究不同模式的相对丰度与临床/生物学参数的相关性。在本病例对照研究中，采用16S宏基因组聚类方法对7例SLE患者和7例健康对照者的细菌谱进行了研究。本研究报告，与对照组相比，SLE样本中芽胞杆菌类（0.58%，对照组1.26%）、乳酸杆菌属（0.43%，对照组0.74%）丰度较低，而γ变形菌属（2.37%，对照组0.77%）和志贺氏杆菌属（2.04%，对照组0.51%）丰度较高

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引用次数: 0

Análisis de muestras de kéfir de agua para el desarrollo de un marco regulatorio en el Código Alimentario Argentino [对含糖开菲尔酒样品的分析，为阿根廷食品法典制定监管框架]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2025.02.002

Manuel Teijeiro , Analía Graciela Abraham , Josefina Cabrera , Galia Ana Suchowlanski , Ana Losada , Silvana Ruarte , Mónica López , Gabriel Vinderola

Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100 ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1 × 10⁷ CFU/ml and a yeast count of 1 × 10⁶ CFU/ml, with enterobacteria counts lower than 10 CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.

水开菲尔是一种发酵饮料，它的生产、商业化和消费越来越受欢迎。然而，《阿根廷食品法典》（CAA，西班牙语首字母缩写）并没有考虑到这一点。这项工作的目的是确定阿根廷企业家生产的水开菲尔样品的一些微生物和物理化学特性，为国家食品委员会（CONAL，西班牙语首字母缩略词）提供必要的信息，以便编写一篇将水开菲尔纳入CAA的文章。为此，邀请了来自该国不同城市的31家水开菲尔生产商将其产品样品送到国家食品研究所（INAL，西班牙语首字母缩写）的国家参考实验室（阿根廷布宜诺斯艾利斯）进行微生物和物理化学分析。在2021年12月至2022年2月期间，共收到13个样品，以及相应的生产过程信息。分析的样品显示pH值在2.9和3.7之间，可滴定酸度在6和16.6之间（ml NaOH 1N/100ml样品），酒精含量在0.58和2.55之间（%v/v），乳酸菌计数为1×107CFU/ml，酵母计数为1×106CFU/ml，肠杆菌计数低于10CFU/ml)。结果提供了当地数据，最终于2024年8月12日公布了卫生质量秘书处和生物经济秘书处的第7/2024号联合决议，其中第1084条tris被纳入CAA关于发酵饮料的第13章，涉及水开菲尔。

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引用次数: 0

Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil 巴西Caatinga生物群系野外条件下山羊钩端螺旋体感染的前瞻性评价。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2025.03.001

Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo

The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.

卡廷加生物群系只存在于巴西；然而，对该地区在田间条件下饲养的山羊，没有使用直接和间接诊断试验对钩端螺旋体病进行调查。我们进行了一项前瞻性调查，采用配对抽样来评估载体山羊在疾病维持中的重要性。在抽样计算的基础上，随机选择60只山羊（30只圈养，30只放养），在雨季监测3个月，每月配对采集生物样本。收集动物尿液和阴道液用于微生物学和分子诊断，并收集血清样本用于显微镜凝集试验（MAT）的血清学诊断。总体而言，45只（75%）动物在至少一种诊断方法中呈阳性。43只（71.7%）动物在3个采样时刻均检测到钩端螺旋体抗体，抗体滴度在25 ~ 800之间，以第1次和第2次采集的Australis（分别为66.7%和40.7%）和第3次采集的Cynopteri（分别为52.6%）血清群最多。没有动物在微生物诊断或阴道液PCR检测中呈阳性；然而，在第一次采集中，只有密闭组的5只（16.7%）动物尿液PCR阳性。两份尿样DNA测序显示与钩端螺旋体有99%的相似性。建议在Caatinga生物群系野外条件下饲养的山羊中采用不同的钩端螺旋体病诊断技术，并建议在较长时间内进行每月采集的进一步研究，以更好地了解钩端螺旋体的流行情况及其随时间的变化。

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引用次数: 0

Development of an enzyme-linked immunosorbent assay for detection of antibodies against infectious bursal disease virus based on a version of the recombinant viral protein 2 基于重组病毒蛋白2的酶联免疫吸附法检测传染性法氏囊病病毒抗体的建立

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.09.003

Leticia Keller, Carina Romanutti, Flavia Adriana Zanetti

Infectious bursal disease virus (IBDV) is the etiological agent of a highly contagious and immunosuppressive disease in chickens. In poultry farms, the level of anti-IBDV antibodies of numerous serum samples must be monitored using fast and simple methodologies. Therefore, the aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA), in an indirect format, using a version of mature viral protein 2 (VP2) of IBDV as coating agent. This recombinant fusion protein (His-VP2) was expressed at high levels in Escherichia coli. Bacterial inclusion bodies containing His-VP2 were successfully recovered using a simple, inexpensive and efficient method, a further purification of recombinant protein by affinity chromatography using immobilized metal chelates being unnecessary. After the VP2-ELISA was optimized, its performance was evaluated using preanalyzed sera from uninfected specific pathogen-free chickens and broilers vaccinated against IBDV in poultry farms, using a commercial ELISA kit. Based on these results, the developed assay proved to be sensitive, specific and in high agreement with the kit available on the market. In addition, the in-house ELISA demonstrated to be reproducible by intra-assay and inter-assay variability studies. In conclusion, VP2-ELISA could be an efficient and low-cost alternative diagnostic method to detect antibodies to IBDV in the poultry industry.

传染性法氏囊病病毒（IBDV）是鸡传染性和免疫抑制性疾病的病原。在家禽养殖场，必须使用快速和简单的方法监测大量血清样本的抗ibdv抗体水平。因此，本研究的目的是建立一种间接形式的酶联免疫吸附试验（ELISA），使用IBDV的成熟病毒蛋白2 （VP2）作为包衣剂。该重组融合蛋白（His-VP2）在大肠杆菌中高水平表达。利用一种简单、廉价、高效的方法成功地回收了含有His-VP2的细菌包膜体，无需再使用固定化金属螯合物亲和层析纯化重组蛋白。优化后的vp2酶联免疫吸附试验，采用预分析的未感染特异性无病原体鸡和家禽养殖场接种IBDV疫苗的肉鸡血清，使用商用酶联免疫吸附测定试剂盒对其性能进行评价。基于这些结果，开发的检测方法被证明是敏感的，特异性的，并且与市场上可用的试剂盒高度一致。此外，内部ELISA通过测定内和测定间的变异性研究证明是可重复的。由此可见，VP2-ELISA可作为一种高效、低成本的IBDV抗体检测方法。

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引用次数: 0

Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen 利用液氮优化金黄色葡萄球菌DNA提取工艺。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.12.006

Mariana B. Galeano , Stefania A. Robaldi , Tania B. Gordillo , Martiniano M. Ricardi , Pablo M. Cassanelli , Rosana O. Pereda , Maria Mercedes Palomino , Paula Maria Tribelli

DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing Staphylococcus aureus cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from S. aureus USA300 and related clinical isolates were purified using phenol–chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.

DNA提取对于进行全基因组测序等程序至关重要，这需要可重复且具有成本效益的方法。由于金黄色葡萄球菌细胞的肽聚糖层对普通治疗具有抗性，因此裂解金黄色葡萄球菌细胞特别具有挑战性。传统的方法包括使用溶葡萄球菌素进行昂贵的酶解。在这里，我们开发了一种利用液氮和机械破坏砂浆的新方法。采用苯酚-氯仿萃取-沉淀法对金黄色葡萄球菌USA300及相关临床分离株进行DNA纯化。证实了DNA的完整性和纯度，获得了适合于各种分子生物学技术的浓度和纯度。利用PCR扩增不同基因片段，验证所采用DNA的质量。这种方法绕过溶葡萄球菌，产生的DNA适用于其他技术。

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引用次数: 0

Composition in phyla from breast milk: Effect of the mode of delivery 母乳中细菌门的组成：分娩方式的影响。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2025.04.001

Shiva Aslani , Seyedeh Neda Mousavi , Atefeh Kazemi Robati , Siamak Heidarzadeh , Somaye Abdollahi Sabet

Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case–control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (p = 0.03, p = 0.02 and p = 0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (p = 0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (p = 0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.

母乳被认为是一个有生命的生态系统。母体、环境和新生儿因素影响牛奶细菌组成。本研究的目的是评估与剖宫产相比，阴道分娩的母亲母乳中的门。在这项单中心病例对照研究中，60名妇女参与。其中一半是阴道分娩，另一半是剖宫产。分娩后3个月采集母乳标本进行DNA提取，采用定量实时链聚合酶反应测定厚壁菌门、拟杆菌门、放线菌门和变形菌门。两组的每日卡路里、蛋白质、脂肪、碳水化合物和纤维摄入量没有显著差异。剖宫产乳中拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）的比例显著高于阴道分娩（p=0.03、p=0.02和p=0.042）。同样，厚壁菌门与拟杆菌门之比显著增加（p=0.02）。经阴道分娩的男婴母亲的乳汁中放线菌数量显著高于女婴（p=0.015）。与阴道分娩相比，剖宫产母亲的母乳显示出主要细菌门群的变化。此外，我们的研究结果表明，婴儿性别是影响某些门数量的有效因素。

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引用次数: 0

Bacteriemia por Salmonella enterica no Typhi atípica, lactosa positiva, lisina decarboxilasa negativa [非典型非Typhi肠炎沙门氏菌菌血症，乳糖阳性，赖氨酸脱羧酶阴性]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.09.007

Susana D. García , María I. Caffer , Marisa N. Almuzara , Ángela M.R. Famiglietti , Cristina Myburg , María R. Viñas , Jorge Risso , Carlos A. Vay

We describe here the first isolation in Argentina of a lactose-fermenting and lysine decarboxylase-negative non-Typhi Salmonella enterica from a blood culture of a patient with systemic lupus erythematosus. The microorganism was identified as S. enterica (score: 2.148) by mass spectrometry, as “Salmonella group” (discrimination: 86%) by the VITEK® 2 system and as Citrobacter freundii complex by biochemical tests. The serological test using the OMA antiserum for the somatic antigen was positive. The identification was confirmed as Salmonella subsp. I 4,[5],12:i:- atypical at the National Reference Center ANLIS Dr. Carlos G. Malbrán. Mass spectrometry is especially useful in microbial identification in the presence of atypia, while biochemical tests can lead to misidentification in these cases. This study warns about the circulation of this strain in Argentina.

我们在此描述了阿根廷首次从一名系统性红斑狼疮患者的血液培养物中分离出一种乳糖发酵和赖氨酸脱羧酶阴性的非泰菲氏肠炎沙门氏菌。该微生物通过质谱鉴定为肠炎沙门氏菌（得分：2.148），通过 VITEK® 2 系统鉴定为 "沙门氏菌群"（辨别率：86%），通过生化检验鉴定为弗氏柠檬酸杆菌复合物。使用体细胞抗原 OMA 抗血清进行的血清学检测呈阳性。卡洛斯-马尔布兰博士（Carlos G. Malbrán）在国家参考资料中心（ANLIS Dr. Carlos G. Malbrán）确认其为 I 4[5],12:i:-非典型沙门氏菌亚种。质谱法在非典型性微生物鉴定中特别有用，而生化检验在这些情况下可能会导致错误鉴定。这项研究对这种菌株在阿根廷的传播提出了警告。

引用次数: 0

In vitro activity of ceftobiprole against Staphylococcus aureus: Current experience 头孢替比罗对金黄色葡萄球菌的体外活性：目前的经验。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 DOI: 10.1016/j.ram.2024.12.011

Manuel Paz-Infanzon , Adrian Camacho-Ortiz , Eduardo Perez-Alba , Laura Nuzzolo-Shihadeh , Rayo Morfin-Otero , Paola Bocanegra-Ibarias

引用次数: 0

Risk factors associated with neonatal sepsis due to Serratia marcescens in a public hospital in Mexico: A case-control study. 墨西哥公立医院粘质沙雷菌所致新生儿败血症的相关危险因素：一项病例对照研究

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-06-11 DOI: 10.1016/j.ram.2025.04.006

Juan Carlos Lona-Reyes, Lucia Miramontes-Buiza, Luis Fernando Frías-Flores, Jose Angel Regla-Nava, Jesús González-Carmona, Juan Antonio Gallegos-Marín, Mariana Chávez-Rodríguez

This study aimed to identify factors associated with neonatal sepsis due to Serratia marcescens in a Neonatal Intensive Care Unit (NICU) in Mexico. A case-control study was conducted, matched by gestational age and birth weight. The analysis was performed using logistic regression. Seventy newborns with S. marcescens sepsis were identified; the median gestational age was 34.4 weeks, and the median birth weight was 1680g. Microbial isolates were identified in the bloodstream in 98.6% of cases, and 88.6% of these were diagnosed after 72h of life. Independent variables associated with infection were the presence of a central venous catheter (OR 5.17; 95% CI 1.57-17.1), mechanical ventilation (OR 3.23; 95% CI 1.18-8.87), and prior use of meropenem (OR 3.38; 95% CI 1.01-11.3). The case fatality rate of the infection was 32.9%.

本研究旨在确定与墨西哥新生儿重症监护病房（NICU）中粘质沙雷菌引起的新生儿败血症相关的因素。进行了一项病例对照研究，与胎龄和出生体重相匹配。采用逻辑回归进行分析。70例新生儿肉质葡萄球菌脓毒症；中位胎龄为34.4周，中位出生体重为1680g。98.6%的病例在血液中发现了分离的微生物，其中88.6%在生命72小时后被诊断出来。与感染相关的独立变量是中心静脉导管的存在(OR 5.17；95% CI 1.57-17.1)，机械通气(OR 3.23；95% CI 1.18-8.87)，既往使用美罗培南(OR 3.38；95% ci 1.01-11.3)。感染病死率为32.9%。

引用次数: 0

Effect of probiotic bacteria, phycocyanin and tarragon essential oil on the physiochemical, microbiological, and sensory properties of a mouth freshener tablet. 益生菌、藻蓝蛋白和龙蒿精油对口腔清新片理化、微生物学和感官特性的影响。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-05-28 DOI: 10.1016/j.ram.2025.04.004

Fatemeh Habibi, Bahareh Nowruzi, Maryam Ghane

Microbial pigments are extensively used in industry as food coloring, dyes, and medicinal substances. This study aimed to develop a probiotic mouth freshener tablet containing PC, tarragon essential oil, and microencapsulated Lactobacillus plantarum and investigate its antimicrobial properties against Streptococcus mutans. First, cyanobacterium Spirulina sp. was cultured and PC extracted. Then, microbial suspension and microencapsulation of probiotic bacteria, tarragon essential oil extraction, as well as mouth freshener tablets were prepared. Four treatments (T) under Co: control sample, T₁, T₂, T₃, and T₄ contained 1% PC, 1% microcoating probiotic, 0.8% tarragon essential oil, and 1% PC+1% microcoating probiotic+0.8% tarragon essential oil, were prepared respectively. The results of the inhibition zone diameter of samples also indicated that the highest and lowest sensitivity were observed in T₄ and T₁, respectively. Based on the results, the addition of PC and tarragon essential oil significantly increased the antioxidant activity of the samples (p<0.05). The results indicated that the release rate of probiotic bacteria under gastric conditions in T₄ was lower compared to that in treatment T₂ (p<0.05). Sensory evaluation results showed that the highest values were observed in T₃ and T₄ (p<0.05). CONCLUSION: Overall, it can be concluded that the prepared mouth freshener tablets (T₄) can be effective agents for oral disinfection and the promotion of public health.

微生物色素在工业上广泛用作食用色素、染料和药用物质。本研究旨在研制一种含有PC、龙蒿精油和植物乳杆菌微胶囊的益生菌口腔清新剂片剂，并研究其对变形链球菌的抗菌性能。首先，培养蓝藻螺旋藻，提取PC；然后，制备了益生菌微生物悬浮液和微胶囊、龙蒿精油提取液以及口腔清新片。Co下4个处理(T)分别为对照样品T1、T2、T3和T4，分别含有1% PC、1%微包衣益生菌、0.8%龙蒿精油和1% PC+1%微包衣益生菌+0.8%龙蒿精油。样品的抑制带直径测定结果也显示，在T4和T1时，其敏感性最高，最低。综上所述，添加PC和龙蒿精油可显著提高样品的抗氧化活性（p4较T2处理低）（p3和T4 (p4)），可作为有效的口腔消毒剂，促进公众健康。

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引用次数: 0