Revista Argentina de microbiologia最新文献_第6页

Reporte de una experiencia de control del virus de la diarrea viral bovina en 2 tambos de Argentina aplicando herramientas de manejo, diagnóstico y vacunación [阿根廷2个奶牛场应用管理、诊断和疫苗接种工具对牛病毒性腹泻病毒的现场控制经验报告]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-01-27 DOI: 10.1016/j.ram.2024.12.001

Rodrigo Pereyra , Fernando Martino , Mariángeles Castillo , Juan Manuel Sala , Lucas José Barone , Claudio Paolazzi , Alejandra Victoria Capozzo

Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (p < 0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.

牛病毒性腹泻病毒（BVDV）在国际畜牧业和阿根廷造成重大经济损失，该病毒在阿根廷的流行率很高。在高流行情况下，一旦发现并隔离持续感染的动物，就可以通过接种疫苗来控制BVDV感染。本研究对圣达菲省2个奶牛场采用诊断、管理和疫苗接种相结合的方法，在田间条件下控制BVDV传播的可行性进行了评价。采用商业elisa检测NS3 （P80）蛋白或针对该蛋白的抗体，rt -巢式PCR检测病毒基因组，并进行病毒血清中和以评估疫苗效力。养殖场平均血清阳性率为58.4%，持续感染动物率为2.4%。分离出持续感染的动物，并给它们接种含有灭活BVDV的商业联合疫苗后，流产率显著降低(p

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引用次数: 0

Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota 麦秸改性过硫酸铵预处理含油污泥及其对本地真菌群的影响。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-06-18 DOI: 10.1016/j.ram.2025.05.002

Natalia Andrea Di Clemente , Marina Peluffo , Ana Carolina Agnello , Deborah Colman , Norma Buceta , María Teresa Del Panno , Mario Carlos Nazareno Saparrat

Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.

许多研究已经解决了细菌群落在响应修复策略时的动态变化，而真菌群落尽管具有作为生物修复剂的潜力，但研究相对较少。本研究的目的是评价小麦秸秆改良剂（WSA）对未经处理和过硫酸铵处理的含油污泥60天后对本地真菌群的影响。18S rRNA扩增子测序、微生物酶活性和化学参数分析。预处理60天后，WSA对预氧化含油污泥的总石油烃（TPH）去除率较高，漆酶活性最高。真菌多样性和公平性也得到了恢复，直到达到与未处理微生物相似的指数值。然而，真菌分类类群发生了变化，在处理结束时，Eurotiales（93.4%）被Microascales（57.9%）和Sordariales（32.8%）所取代。我们的研究结果表明，含油污泥中易于同化的有机物的输入可能加速真菌分类群的变化和替换，也影响微生物的定植，从而影响污染物的去除。这些发现强调了将真菌动力学纳入生物修复策略作为含油污泥处理的补充方法的相关性。

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引用次数: 0

Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus 突尼斯系统性红斑狼疮患者16S宏基因组学微生物多样性研究。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-06-03 DOI: 10.1016/j.ram.2025.04.002

Amira Messadi , Sameh Sayhi , Kais Ghedira , Chadia Zaouaoui , Bilel Arfaoui , Soumaya Khouikhi , Maha Rebai , Nour El Houda Guediche , Nadia Abdelhafidh , Bassem Louzir , Ajili Faida

Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case–control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus Lactobacillus (0.43% vs 0.74%), as well as a higher abundance of the genera Gammaproteobacteria (2.37% vs 0.77%) and Escherichia–Shigella (2.04% vs 0.51%) in SLE samples compared to the controls (p < 0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus Faecalibacterium (11.34% vs 3.35%) and renal manifestations (p < 0.05). The phylum Actinobacteria (0.21% vs 3.8%, p = 0.036) and the genus Bifidobacterium levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case–control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.

系统性红斑狼疮（SLE）是一种多系统自身免疫性疾病，具有显著的发病率和死亡率。它的特点是丧失自身免疫耐受性和自身抗体的产生，导致多器官损伤。新出现的研究已经证实了肠道菌群失调在SLE患者中的作用，尽管其潜在机制迄今尚不清楚。在这项研究中，我们旨在调查SLE的细菌谱，包括门/类/属的相对丰度和多样性，并将其与健康对照进行比较，并研究不同模式的相对丰度与临床/生物学参数的相关性。在本病例对照研究中，采用16S宏基因组聚类方法对7例SLE患者和7例健康对照者的细菌谱进行了研究。本研究报告，与对照组相比，SLE样本中芽胞杆菌类（0.58%，对照组1.26%）、乳酸杆菌属（0.43%，对照组0.74%）丰度较低，而γ变形菌属（2.37%，对照组0.77%）和志贺氏杆菌属（2.04%，对照组0.51%）丰度较高

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引用次数: 0

Clinical and demographic characteristics of spondylodiscitis in a Mexican population: A retrospective study 墨西哥人群脊柱炎的临床和人口学特征：一项回顾性研究。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-02-25 DOI: 10.1016/j.ram.2025.01.002

Mario Alberto Dueñas-Espinosa , Julio César López-Valdés , Daniel Alejandro Vega-Moreno , María Fernanda Guzmán-del Río , Rafael Sánchez-Mata , María Elena Córdoba-Mosqueda , Laura Mestre-Orozco , Erick Alberto Castañeda-Ramírez , Alejandro Jacob Madrid-Sánchez , Ulises García-González

The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's t-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0 ± 11.5 years (range: 38–83), and the average body mass index (BMI) was 28 ± 4.05 kg/m². Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were Staphylococcus aureus (28 cases) and Escherichia coli (21 cases). In 16 cases, intracellular pathogens such as Mycobacterium tuberculosis and Brucella spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.

本研究的目的是通过微生物培养确定确诊为脊椎椎间盘炎的患者的临床和人口学特征。进行了一项描述性、观察性和回顾性研究。纳入患者的依据是椎体感染、未明确的椎间盘炎和/或与椎间盘炎一致的微生物培养阳性的临床和影像学证据。对于男女之间的比较，采用了学生t检验和优势比。卡方检验用于检查受影响的脊柱水平、分离的微生物和相关合并症之间的相关性。共发现86例椎间盘炎，65%为男性患者。平均年龄59.0±11.5岁（范围38 ~ 83岁），平均体重指数（BMI） 28±4.05kg/m2。68%的病例以原发性椎间盘炎为主，主要发生在胸部。17例患者表现为与慢性退行性疾病无关的脊椎椎间盘炎。最常见的分离微生物是金黄色葡萄球菌（28例）和大肠杆菌（21例）。在16例中，发现了细胞内病原体，如结核分枝杆菌和布鲁氏菌，导致平均住院30天。脊柱炎是一种严重的并发症，这项研究强调了与以前发表的数据的差异，特别是在涉及的微生物和人口统计资料方面。

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引用次数: 0

Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil 巴西Caatinga生物群系野外条件下山羊钩端螺旋体感染的前瞻性评价。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-04-07 DOI: 10.1016/j.ram.2025.03.001

Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo

The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.

卡廷加生物群系只存在于巴西；然而，对该地区在田间条件下饲养的山羊，没有使用直接和间接诊断试验对钩端螺旋体病进行调查。我们进行了一项前瞻性调查，采用配对抽样来评估载体山羊在疾病维持中的重要性。在抽样计算的基础上，随机选择60只山羊（30只圈养，30只放养），在雨季监测3个月，每月配对采集生物样本。收集动物尿液和阴道液用于微生物学和分子诊断，并收集血清样本用于显微镜凝集试验（MAT）的血清学诊断。总体而言，45只（75%）动物在至少一种诊断方法中呈阳性。43只（71.7%）动物在3个采样时刻均检测到钩端螺旋体抗体，抗体滴度在25 ~ 800之间，以第1次和第2次采集的Australis（分别为66.7%和40.7%）和第3次采集的Cynopteri（分别为52.6%）血清群最多。没有动物在微生物诊断或阴道液PCR检测中呈阳性；然而，在第一次采集中，只有密闭组的5只（16.7%）动物尿液PCR阳性。两份尿样DNA测序显示与钩端螺旋体有99%的相似性。建议在Caatinga生物群系野外条件下饲养的山羊中采用不同的钩端螺旋体病诊断技术，并建议在较长时间内进行每月采集的进一步研究，以更好地了解钩端螺旋体的流行情况及其随时间的变化。

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引用次数: 0

Análisis de muestras de kéfir de agua para el desarrollo de un marco regulatorio en el Código Alimentario Argentino [对含糖开菲尔酒样品的分析，为阿根廷食品法典制定监管框架]。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-03-24 DOI: 10.1016/j.ram.2025.02.002

Manuel Teijeiro , Analía Graciela Abraham , Josefina Cabrera , Galia Ana Suchowlanski , Ana Losada , Silvana Ruarte , Mónica López , Gabriel Vinderola

Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100 ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1 × 10⁷ CFU/ml and a yeast count of 1 × 10⁶ CFU/ml, with enterobacteria counts lower than 10 CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.

水开菲尔是一种发酵饮料，它的生产、商业化和消费越来越受欢迎。然而，《阿根廷食品法典》（CAA，西班牙语首字母缩写）并没有考虑到这一点。这项工作的目的是确定阿根廷企业家生产的水开菲尔样品的一些微生物和物理化学特性，为国家食品委员会（CONAL，西班牙语首字母缩略词）提供必要的信息，以便编写一篇将水开菲尔纳入CAA的文章。为此，邀请了来自该国不同城市的31家水开菲尔生产商将其产品样品送到国家食品研究所（INAL，西班牙语首字母缩写）的国家参考实验室（阿根廷布宜诺斯艾利斯）进行微生物和物理化学分析。在2021年12月至2022年2月期间，共收到13个样品，以及相应的生产过程信息。分析的样品显示pH值在2.9和3.7之间，可滴定酸度在6和16.6之间（ml NaOH 1N/100ml样品），酒精含量在0.58和2.55之间（%v/v），乳酸菌计数为1×107CFU/ml，酵母计数为1×106CFU/ml，肠杆菌计数低于10CFU/ml)。结果提供了当地数据，最终于2024年8月12日公布了卫生质量秘书处和生物经济秘书处的第7/2024号联合决议，其中第1084条tris被纳入CAA关于发酵饮料的第13章，涉及水开菲尔。

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引用次数: 0

Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen 利用液氮优化金黄色葡萄球菌DNA提取工艺。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-01-27 DOI: 10.1016/j.ram.2024.12.006

Mariana B. Galeano , Stefania A. Robaldi , Tania B. Gordillo , Martiniano M. Ricardi , Pablo M. Cassanelli , Rosana O. Pereda , Maria Mercedes Palomino , Paula Maria Tribelli

DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing Staphylococcus aureus cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from S. aureus USA300 and related clinical isolates were purified using phenol–chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.

DNA提取对于进行全基因组测序等程序至关重要，这需要可重复且具有成本效益的方法。由于金黄色葡萄球菌细胞的肽聚糖层对普通治疗具有抗性，因此裂解金黄色葡萄球菌细胞特别具有挑战性。传统的方法包括使用溶葡萄球菌素进行昂贵的酶解。在这里，我们开发了一种利用液氮和机械破坏砂浆的新方法。采用苯酚-氯仿萃取-沉淀法对金黄色葡萄球菌USA300及相关临床分离株进行DNA纯化。证实了DNA的完整性和纯度，获得了适合于各种分子生物学技术的浓度和纯度。利用PCR扩增不同基因片段，验证所采用DNA的质量。这种方法绕过溶葡萄球菌，产生的DNA适用于其他技术。

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引用次数: 0

Development of an enzyme-linked immunosorbent assay for detection of antibodies against infectious bursal disease virus based on a version of the recombinant viral protein 2 基于重组病毒蛋白2的酶联免疫吸附法检测传染性法氏囊病病毒抗体的建立

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-01-22 DOI: 10.1016/j.ram.2024.09.003

Leticia Keller, Carina Romanutti, Flavia Adriana Zanetti

Infectious bursal disease virus (IBDV) is the etiological agent of a highly contagious and immunosuppressive disease in chickens. In poultry farms, the level of anti-IBDV antibodies of numerous serum samples must be monitored using fast and simple methodologies. Therefore, the aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA), in an indirect format, using a version of mature viral protein 2 (VP2) of IBDV as coating agent. This recombinant fusion protein (His-VP2) was expressed at high levels in Escherichia coli. Bacterial inclusion bodies containing His-VP2 were successfully recovered using a simple, inexpensive and efficient method, a further purification of recombinant protein by affinity chromatography using immobilized metal chelates being unnecessary. After the VP2-ELISA was optimized, its performance was evaluated using preanalyzed sera from uninfected specific pathogen-free chickens and broilers vaccinated against IBDV in poultry farms, using a commercial ELISA kit. Based on these results, the developed assay proved to be sensitive, specific and in high agreement with the kit available on the market. In addition, the in-house ELISA demonstrated to be reproducible by intra-assay and inter-assay variability studies. In conclusion, VP2-ELISA could be an efficient and low-cost alternative diagnostic method to detect antibodies to IBDV in the poultry industry.

传染性法氏囊病病毒（IBDV）是鸡传染性和免疫抑制性疾病的病原。在家禽养殖场，必须使用快速和简单的方法监测大量血清样本的抗ibdv抗体水平。因此，本研究的目的是建立一种间接形式的酶联免疫吸附试验（ELISA），使用IBDV的成熟病毒蛋白2 （VP2）作为包衣剂。该重组融合蛋白（His-VP2）在大肠杆菌中高水平表达。利用一种简单、廉价、高效的方法成功地回收了含有His-VP2的细菌包膜体，无需再使用固定化金属螯合物亲和层析纯化重组蛋白。优化后的vp2酶联免疫吸附试验，采用预分析的未感染特异性无病原体鸡和家禽养殖场接种IBDV疫苗的肉鸡血清，使用商用酶联免疫吸附测定试剂盒对其性能进行评价。基于这些结果，开发的检测方法被证明是敏感的，特异性的，并且与市场上可用的试剂盒高度一致。此外，内部ELISA通过测定内和测定间的变异性研究证明是可重复的。由此可见，VP2-ELISA可作为一种高效、低成本的IBDV抗体检测方法。

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引用次数: 0

Composition in phyla from breast milk: Effect of the mode of delivery 母乳中细菌门的组成：分娩方式的影响。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-05-22 DOI: 10.1016/j.ram.2025.04.001

Shiva Aslani , Seyedeh Neda Mousavi , Atefeh Kazemi Robati , Siamak Heidarzadeh , Somaye Abdollahi Sabet

Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case–control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (p = 0.03, p = 0.02 and p = 0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (p = 0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (p = 0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.

母乳被认为是一个有生命的生态系统。母体、环境和新生儿因素影响牛奶细菌组成。本研究的目的是评估与剖宫产相比，阴道分娩的母亲母乳中的门。在这项单中心病例对照研究中，60名妇女参与。其中一半是阴道分娩，另一半是剖宫产。分娩后3个月采集母乳标本进行DNA提取，采用定量实时链聚合酶反应测定厚壁菌门、拟杆菌门、放线菌门和变形菌门。两组的每日卡路里、蛋白质、脂肪、碳水化合物和纤维摄入量没有显著差异。剖宫产乳中拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）的比例显著高于阴道分娩（p=0.03、p=0.02和p=0.042）。同样，厚壁菌门与拟杆菌门之比显著增加（p=0.02）。经阴道分娩的男婴母亲的乳汁中放线菌数量显著高于女婴（p=0.015）。与阴道分娩相比，剖宫产母亲的母乳显示出主要细菌门群的变化。此外，我们的研究结果表明，婴儿性别是影响某些门数量的有效因素。

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引用次数: 0

In vitro activity of ceftobiprole against Staphylococcus aureus: Current experience 头孢替比罗对金黄色葡萄球菌的体外活性：目前的经验。

IF 1.8 4区生物学 Q4 MICROBIOLOGY

Revista Argentina de microbiologia

Pub Date : 2025-07-01 Epub Date: 2025-03-04 DOI: 10.1016/j.ram.2024.12.011

Manuel Paz-Infanzon , Adrian Camacho-Ortiz , Eduardo Perez-Alba , Laura Nuzzolo-Shihadeh , Rayo Morfin-Otero , Paola Bocanegra-Ibarias

引用次数: 0