Pub Date : 2025-07-01Epub Date: 2025-06-18DOI: 10.1016/j.ram.2025.05.002
Natalia Andrea Di Clemente , Marina Peluffo , Ana Carolina Agnello , Deborah Colman , Norma Buceta , María Teresa Del Panno , Mario Carlos Nazareno Saparrat
Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.
{"title":"Wheat straw amendment to an oily sludge pretreated with ammonium persulfate and its impact on the indigenous mycobiota","authors":"Natalia Andrea Di Clemente , Marina Peluffo , Ana Carolina Agnello , Deborah Colman , Norma Buceta , María Teresa Del Panno , Mario Carlos Nazareno Saparrat","doi":"10.1016/j.ram.2025.05.002","DOIUrl":"10.1016/j.ram.2025.05.002","url":null,"abstract":"<div><div>Numerous studies have addressed the dynamics of bacterial communities in response to remediation strategies, while fungal communities, despite their potential as bioremediation agents, remain comparatively understudied. The aim of this work was to evaluate the impact of wheat straw amendment (WSA) on an oily sludge untreated and treated with ammonium persulfate on indigenous mycobiota after 60 days. Culture-independent 18S rRNA amplicon sequencing, microbial enzymatic activity and chemical parameters were analyzed. WSA on a preoxidized oily sludge promoted high total petroleum hydrocarbon (TPH) removal after 60 days, exhibiting the highest laccase activity. Fungal diversity and equitability were also recovered until reaching similar index values to those of the untreated microcosms. However, changes in fungal taxonomical groups were detected, with Eurotiales (93.4%) being replaced by Microascales (57.9%) and Sordariales (32.8%) at the end of the treatment. Our results suggest that inputs of easily assimilable organic matter in oily sludge might accelerate changes and replacement of fungal taxa, also affecting microbial colonization and, consequently, pollutant removal. These findings highlight the relevance of incorporating fungal dynamics into bioremediation strategies as a complementary approach to oily sludge treatment.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 288-297"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-01-27DOI: 10.1016/j.ram.2024.12.001
Rodrigo Pereyra , Fernando Martino , Mariángeles Castillo , Juan Manuel Sala , Lucas José Barone , Claudio Paolazzi , Alejandra Victoria Capozzo
Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (p < 0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.
{"title":"Reporte de una experiencia de control del virus de la diarrea viral bovina en 2 tambos de Argentina aplicando herramientas de manejo, diagnóstico y vacunación","authors":"Rodrigo Pereyra , Fernando Martino , Mariángeles Castillo , Juan Manuel Sala , Lucas José Barone , Claudio Paolazzi , Alejandra Victoria Capozzo","doi":"10.1016/j.ram.2024.12.001","DOIUrl":"10.1016/j.ram.2024.12.001","url":null,"abstract":"<div><div>Bovine viral diarrhea virus (BVDV) causes significant economic losses in the international livestock industry and in Argentina, where it circulates at high prevalence. Under high prevalence conditions, BVDV infections are controlled through vaccination once persistently infected animals are identified and segregated. This study evaluated the feasibility of controlling BVDV circulation under field conditions by combining diagnosis, management measures, and vaccination in 2 dairy farms in the province of Santa Fe. Commercial ELISAs were used for the detection of the NS3 (P80) protein or antibodies against this protein as well as an RT-nested PCR for the detection of the viral genome, and viral seroneutralization to assess vaccine efficacy. The average seroprevalence of the farms was 58.4%, with a persistently infected animal rate of 2.4%. After segregating the persistently infected animals and vaccinating them with a commercial combined vaccine containing inactivated BVDV, abortion rates significantly decreased (<em>p</em> <!--><<!--> <!-->0.05) in farm 1 (from 20.5 to 11.6%) and in farm 2 (from 34 to 23.4%) during the second year of the control strategy. Conception rates increased from 29 to 33% in farm 1 during the first year, while in farm 2, the increase was 7 points during the second year. This methodology achieved conditions in which BVDV ceased to circulate, constituting the first controlled report on BVDV management in dairy farms using tools available to producers in Argentina.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 241-250"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case–control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus Lactobacillus (0.43% vs 0.74%), as well as a higher abundance of the genera Gammaproteobacteria (2.37% vs 0.77%) and Escherichia–Shigella (2.04% vs 0.51%) in SLE samples compared to the controls (p < 0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus Faecalibacterium (11.34% vs 3.35%) and renal manifestations (p < 0.05). The phylum Actinobacteria (0.21% vs 3.8%, p = 0.036) and the genus Bifidobacterium levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case–control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.
{"title":"Microbial diversity investigation using 16S metagenomics in Tunisian patients with systemic lupus erythematosus","authors":"Amira Messadi , Sameh Sayhi , Kais Ghedira , Chadia Zaouaoui , Bilel Arfaoui , Soumaya Khouikhi , Maha Rebai , Nour El Houda Guediche , Nadia Abdelhafidh , Bassem Louzir , Ajili Faida","doi":"10.1016/j.ram.2025.04.002","DOIUrl":"10.1016/j.ram.2025.04.002","url":null,"abstract":"<div><div>Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease associated with significant morbidity and mortality. It is characterized by a loss of self-immune tolerance and autoantibody production, leading to multiple organ damage. Emerging investigations have confirmed the role of gut microbiota dysbiosis in patients with SLE, although the underlying mechanisms remain unclear to date. In this study, we aim to investigate the bacterial profile of SLE including phylum/class/genus relative abundance and diversity, to compare them with healthy controls and to study the correlation of relative abundance of different patterns with clinical/biological parameters. In this case–control study, the bacterial profile was investigated in 7 SLE patients and 7 healthy controls using 16S metagenomics clustering. The present study reported a low abundance of the class Bacilli (0.58% in SLE vs 1.26% in the controls), the genus <em>Lactobacillus</em> (0.43% vs 0.74%), as well as a higher abundance of the genera <em>Gammaproteobacteria</em> (2.37% vs 0.77%) and <em>Escherichia</em>–<em>Shigella</em> (2.04% vs 0.51%) in SLE samples compared to the controls (<em>p</em> <!--><<!--> <!-->0.05). We also found an association between the class Betaproteobacteria (4.42% vs 1.57%) and the genus <em>Faecalibacterium</em> (11.34% vs 3.35%) and renal manifestations (<em>p</em> <!--><<!--> <!-->0.05). The phylum Actinobacteria (0.21% vs 3.8%, <em>p</em> <!-->=<!--> <!-->0.036) and the genus <em>Bifidobacterium</em> levels were lower in active SLE compared to the healthy controls. This study is the first report on the gut microbiota of SLE and the first case–control study in Tunisia and North Africa. We obtained a particular profile of bacterial gut microbiota for the SLE group. We found a specific clustering when compared to the healthy controls.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 275-287"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-02-25DOI: 10.1016/j.ram.2025.01.002
Mario Alberto Dueñas-Espinosa , Julio César López-Valdés , Daniel Alejandro Vega-Moreno , María Fernanda Guzmán-del Río , Rafael Sánchez-Mata , María Elena Córdoba-Mosqueda , Laura Mestre-Orozco , Erick Alberto Castañeda-Ramírez , Alejandro Jacob Madrid-Sánchez , Ulises García-González
The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's t-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0 ± 11.5 years (range: 38–83), and the average body mass index (BMI) was 28 ± 4.05 kg/m2. Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were Staphylococcus aureus (28 cases) and Escherichia coli (21 cases). In 16 cases, intracellular pathogens such as Mycobacterium tuberculosis and Brucella spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.
{"title":"Clinical and demographic characteristics of spondylodiscitis in a Mexican population: A retrospective study","authors":"Mario Alberto Dueñas-Espinosa , Julio César López-Valdés , Daniel Alejandro Vega-Moreno , María Fernanda Guzmán-del Río , Rafael Sánchez-Mata , María Elena Córdoba-Mosqueda , Laura Mestre-Orozco , Erick Alberto Castañeda-Ramírez , Alejandro Jacob Madrid-Sánchez , Ulises García-González","doi":"10.1016/j.ram.2025.01.002","DOIUrl":"10.1016/j.ram.2025.01.002","url":null,"abstract":"<div><div>The aim of this study was to identify the clinical and demographic characteristics of patients with a confirmed diagnosis of spondylodiscitis through microbiological cultures. A descriptive, observational, and retrospective study was conducted. Patients were included based on clinical and radiological evidence of vertebral infection, unspecified discitis, and/or positive microbiological cultures consistent with spondylodiscitis. For the comparison between men and women, the Student's <em>t</em>-test and odds ratio were employed. The Chi-square test was used to examine correlations between affected spinal levels, isolated microorganisms, and associated comorbidities. A total of 86 cases of discitis were identified, 65% of which involved male patients. The mean age was 59.0<!--> <!-->±<!--> <!-->11.5 years (range: 38–83), and the average body mass index (BMI) was 28<!--> <!-->±<!--> <!-->4.05<!--> <!-->kg/m<sup>2</sup>. Primary discitis predominated in 68% of cases, mainly at the thoracic level. Seventeen patients presented with spondylodiscitis not associated with chronic degenerative diseases. The most frequently isolated microorganisms were <em>Staphylococcus aureus</em> (28 cases) and <em>Escherichia coli</em> (21 cases). In 16 cases, intracellular pathogens such as <em>Mycobacterium tuberculosis</em> and <em>Brucella</em> spp. were identified, leading to an average hospital stay of 30 days. Spondylodiscitis is a serious complication, and this study highlights differences from previously published data, particularly in terms of the microorganisms involved and the demographic profile of the population.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 256-264"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-04-07DOI: 10.1016/j.ram.2025.03.001
Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo
The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.
{"title":"Prospective evaluation of Leptospira spp. infection in goats maintained in field conditions in the Caatinga biome, Brazil","authors":"Mayla de Lisbôa Padilha, Lídio Ricardo Bezerra de Melo, Clécio Henrique Limeira, Nathália Maria de Andrade Magalhães, Maria Luana Cristiny Rodrigues Silva, Rafael Rodrigues Soares, Clebert José Alves, Severino Silvano dos Santos Higino, Carolina de Sousa Américo Batista Santos, Sérgio Santos de Azevedo","doi":"10.1016/j.ram.2025.03.001","DOIUrl":"10.1016/j.ram.2025.03.001","url":null,"abstract":"<div><div>The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-<em>Leptospira</em> spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were <em>Australis</em> in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and <em>Cynopteri</em> in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with <em>Leptospira interrogans</em>. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of <em>Leptospira</em> spp. and its variation over time.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 265-274"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-03-24DOI: 10.1016/j.ram.2025.02.002
Manuel Teijeiro , Analía Graciela Abraham , Josefina Cabrera , Galia Ana Suchowlanski , Ana Losada , Silvana Ruarte , Mónica López , Gabriel Vinderola
Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100 ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1 × 107 CFU/ml and a yeast count of 1 × 106 CFU/ml, with enterobacteria counts lower than 10 CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.
{"title":"Análisis de muestras de kéfir de agua para el desarrollo de un marco regulatorio en el Código Alimentario Argentino","authors":"Manuel Teijeiro , Analía Graciela Abraham , Josefina Cabrera , Galia Ana Suchowlanski , Ana Losada , Silvana Ruarte , Mónica López , Gabriel Vinderola","doi":"10.1016/j.ram.2025.02.002","DOIUrl":"10.1016/j.ram.2025.02.002","url":null,"abstract":"<div><div>Water kefir is a fermented beverage that has experienced a growing popular interest in its production, commercialization and consumption. However, it was not contemplated as such in the Argentine Food Code (CAA, Spanish acronym). The aim of this work was to determine some microbiological and physicochemical characteristics of water kefir samples produced by Argentine entrepreneurs, to have the necessary information for the National Food Commission (CONAL, Spanish acronym) to generate an article incorporating water kefir in the CAA. For this purpose, 31 water kefir producers from different cities of the country were invited to send samples of their products for microbiological and physicochemical analysis to the National Reference Laboratory of the National Food Institute (INAL, Spanish acronym) (Buenos Aires, Argentina). In the period December 2021-February 2022, a total of 13 samples were received, together with the corresponding information on their production process. The samples analyzed showed a pH between 2.9 and 3.7, a titratable acidity between 6 and 16.6 (ml of NaOH 1N/100<!--> <!-->ml of sample), an alcohol content between 0.58 and 2.55 (%v/v), a lactic acid bacteria count of 1<!--> <!-->×<!--> <!-->10<sup>7</sup> <!-->CFU/ml and a yeast count of 1<!--> <!-->×<!--> <!-->10<sup>6</sup> <!-->CFU/ml, with enterobacteria counts lower than 10<!--> <!-->CFU/ml). The results provided local data that culminated in the publication, on August 12, 2024, of the joint resolution 7/2024 of the Secretariat of Quality in Health and Secretariat of Bioeconomy by which article 1084 tris was incorporated to the CAA in the chapter XIII of fermented beverages, referring to water kefir.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 298-303"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01Epub Date: 2025-01-27DOI: 10.1016/j.ram.2024.12.006
Mariana B. Galeano , Stefania A. Robaldi , Tania B. Gordillo , Martiniano M. Ricardi , Pablo M. Cassanelli , Rosana O. Pereda , Maria Mercedes Palomino , Paula Maria Tribelli
DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing Staphylococcus aureus cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from S. aureus USA300 and related clinical isolates were purified using phenol–chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.
{"title":"Optimized DNA extraction protocol for Staphylococcus aureus strains utilizing liquid nitrogen","authors":"Mariana B. Galeano , Stefania A. Robaldi , Tania B. Gordillo , Martiniano M. Ricardi , Pablo M. Cassanelli , Rosana O. Pereda , Maria Mercedes Palomino , Paula Maria Tribelli","doi":"10.1016/j.ram.2024.12.006","DOIUrl":"10.1016/j.ram.2024.12.006","url":null,"abstract":"<div><div>DNA extraction is crucial for conducting procedures, such as whole-genome sequencing, which demand methods that are reproducible and cost-effective. Lysing <em>Staphylococcus aureus</em> cells is particularly challenging due to their peptidoglycan layer that is resistant to common treatments. Traditional methods involve costly enzymatic lysis using lysostaphin. Here, we developed a novel approach for lysis utilizing liquid nitrogen and mechanical disruption in a mortar. DNA from <em>S. aureus</em> USA300 and related clinical isolates were purified using phenol–chloroform extraction followed by precipitation. The integrity and purity of DNA were confirmed, obtaining suitable concentration and purity for various molecular biology techniques. The quality of the employed DNA was validated by amplifying fragments of different genes using PCR. This method circumvents lysostaphin, yielding DNA that is suitable for use in other techniques.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 217-220"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infectious bursal disease virus (IBDV) is the etiological agent of a highly contagious and immunosuppressive disease in chickens. In poultry farms, the level of anti-IBDV antibodies of numerous serum samples must be monitored using fast and simple methodologies. Therefore, the aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA), in an indirect format, using a version of mature viral protein 2 (VP2) of IBDV as coating agent. This recombinant fusion protein (His-VP2) was expressed at high levels in Escherichia coli. Bacterial inclusion bodies containing His-VP2 were successfully recovered using a simple, inexpensive and efficient method, a further purification of recombinant protein by affinity chromatography using immobilized metal chelates being unnecessary. After the VP2-ELISA was optimized, its performance was evaluated using preanalyzed sera from uninfected specific pathogen-free chickens and broilers vaccinated against IBDV in poultry farms, using a commercial ELISA kit. Based on these results, the developed assay proved to be sensitive, specific and in high agreement with the kit available on the market. In addition, the in-house ELISA demonstrated to be reproducible by intra-assay and inter-assay variability studies. In conclusion, VP2-ELISA could be an efficient and low-cost alternative diagnostic method to detect antibodies to IBDV in the poultry industry.
{"title":"Development of an enzyme-linked immunosorbent assay for detection of antibodies against infectious bursal disease virus based on a version of the recombinant viral protein 2","authors":"Leticia Keller, Carina Romanutti, Flavia Adriana Zanetti","doi":"10.1016/j.ram.2024.09.003","DOIUrl":"10.1016/j.ram.2024.09.003","url":null,"abstract":"<div><div>Infectious bursal disease virus (IBDV) is the etiological agent of a highly contagious and immunosuppressive disease in chickens. In poultry farms, the level of anti-IBDV antibodies of numerous serum samples must be monitored using fast and simple methodologies. Therefore, the aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA), in an indirect format, using a version of mature viral protein 2 (VP2) of IBDV as coating agent. This recombinant fusion protein (His-VP2) was expressed at high levels in <em>Escherichia coli</em>. Bacterial inclusion bodies containing His-VP2 were successfully recovered using a simple, inexpensive and efficient method, a further purification of recombinant protein by affinity chromatography using immobilized metal chelates being unnecessary. After the VP2-ELISA was optimized, its performance was evaluated using preanalyzed sera from uninfected specific pathogen-free chickens and broilers vaccinated against IBDV in poultry farms, using a commercial ELISA kit. Based on these results, the developed assay proved to be sensitive, specific and in high agreement with the kit available on the market. In addition, the in-house ELISA demonstrated to be reproducible by intra-assay and inter-assay variability studies. In conclusion, VP2-ELISA could be an efficient and low-cost alternative diagnostic method to detect antibodies to IBDV in the poultry industry.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 208-216"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case–control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (p = 0.03, p = 0.02 and p = 0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (p = 0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (p = 0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.
{"title":"Composition in phyla from breast milk: Effect of the mode of delivery","authors":"Shiva Aslani , Seyedeh Neda Mousavi , Atefeh Kazemi Robati , Siamak Heidarzadeh , Somaye Abdollahi Sabet","doi":"10.1016/j.ram.2025.04.001","DOIUrl":"10.1016/j.ram.2025.04.001","url":null,"abstract":"<div><div>Breast milk is considered as a living ecosystem. Maternal, environmental and neonatal factors affect milk bacterial composition. The aim of present study was to assess the phyla from breast milk of mothers with vaginal delivery compared to the cesarean section. In this single-center case–control study, sixty women were participated. Half of them had vaginal delivery and others experienced cesarean section. The breast milk samples were collected three months after delivery for the DNA extraction to measure Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria using quantitative real time chain polymerase reaction. Daily intake of calories, protein, fat, carbohydrate and fiber did not differ significantly between the two groups. The proportion of Bacteroidetes, Firmicutes and Proteobacteria were significantly higher in milk of mothers with a cesarean section than the vaginal delivery (<em>p</em> <!-->=<!--> <!-->0.03, <em>p</em> <!-->=<!--> <!-->0.02 and <em>p</em> <!-->=<!--> <!-->0.042). Similarly, the Firmicutes to Bacteroides ratio was significantly increased (<em>p</em> <!-->=<!--> <!-->0.02). The Actinobacteria population was significantly higher in milk of vaginally-delivered mothers who had male infant than females (<em>p</em> <!-->=<!--> <!-->0.015). Breast milk of mothers with cesarean section showed alterations in the main bacterial phyla population compared to the vaginal delivery. Moreover, our results suggest that the sex of infant is an effective factor on some phyla quantity.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 3","pages":"Pages 231-238"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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