Pub Date : 2025-10-01DOI: 10.1016/j.ram.2025.11.002
Jos Houbraken
{"title":"Future challenges in food mycology – food spoilage, safety and security","authors":"Jos Houbraken","doi":"10.1016/j.ram.2025.11.002","DOIUrl":"10.1016/j.ram.2025.11.002","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 305-306"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145610447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.ram.2025.09.005
Victor Ludeña-Meléndez , Jussara Malo-Castillo , Paula Ishikawa-Arias , Harold Jimenez-Alvarez , Criss Ocampo , Cristhian Guevara-Coronel , Leonardo Ledesma Chavarria , Meilyn Lujan-Benites , Cristhian Terrones , Christian Eduardo Vilca Avalos , Maria Soledad Ayala Ravelo
Academic stress is a factor that impacts the health of medical students by altering their body's internal environment and its various constituent systems, including the microbiota of the digestive system, particularly that of the oral cavity. Therefore, the objective of this study was to evaluate the variation in the facultative oral microbiota of medical students during periods of perceived academic stress during evaluation periods. This was an observational, analytical study that used mouthwash samples and microbiological colony-forming units counts per ml (CFU/ml) of oral bacterial and fungal microorganisms. Forty-seven participants were included: 59.57% were male, 55.32% had good oral hygiene habits, and 93.62% belonged to a middle or low socioeconomic status. The academic stress score was higher in response to the stressful event (UAE = 62.5 ± 12.3) and was significantly associated with a higher CFU/ml count (p <0.05). Yeast colonies corresponding to Candida albicans, C. tropicalis, C. glabrata, and Candida spp. were identified on CHROMagarTM. It is concluded that there is an association between perceived academic stress and socioeconomic status with the variation in the bacterial and fungal oral microbiota, respectively.
{"title":"Variación de la microbiota oral facultativa durante períodos de estrés académico","authors":"Victor Ludeña-Meléndez , Jussara Malo-Castillo , Paula Ishikawa-Arias , Harold Jimenez-Alvarez , Criss Ocampo , Cristhian Guevara-Coronel , Leonardo Ledesma Chavarria , Meilyn Lujan-Benites , Cristhian Terrones , Christian Eduardo Vilca Avalos , Maria Soledad Ayala Ravelo","doi":"10.1016/j.ram.2025.09.005","DOIUrl":"10.1016/j.ram.2025.09.005","url":null,"abstract":"<div><div>Academic stress is a factor that impacts the health of medical students by altering their body's internal environment and its various constituent systems, including the microbiota of the digestive system, particularly that of the oral cavity. Therefore, the objective of this study was to evaluate the variation in the facultative oral microbiota of medical students during periods of perceived academic stress during evaluation periods. This was an observational, analytical study that used mouthwash samples and microbiological colony-forming units counts per ml (CFU/ml) of oral bacterial and fungal microorganisms. Forty-seven participants were included: 59.57% were male, 55.32% had good oral hygiene habits, and 93.62% belonged to a middle or low socioeconomic status. The academic stress score was higher in response to the stressful event (UAE<!--> <!-->=<!--> <!-->62.5<!--> <!-->±<!--> <!-->12.3) and was significantly associated with a higher CFU/ml count <em>(p</em> <0.05). Yeast colonies corresponding to <em>Candida albicans</em>, <em>C. tropicalis, C. glabrata</em>, and <em>Candida</em> spp. were identified on CHROMagar<sup>TM</sup>. It is concluded that there is an association between perceived academic stress and socioeconomic status with the variation in the bacterial and fungal oral microbiota, respectively.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 318-326"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.ram.2025.06.002
Marta Sanchis , Laura Camuña-Pardo , Laia Martínez Quijada , Altea Pérez Escobar , Frederic Gómez-Bertomeu , Alberto Miguel Stchigel Glikman
Two of the three most prevalent bacterial genera associated with human diseases caused by meat consumption are Campylobacter (mainly in poultry meat) and Yersinia (in pork meat). As its detection and quantification is not regulated by the European legislation (except for the quantification of Campylobacter in poultry channels), several samples of chicken burgers from different establishments including supermarkets and retail trade outlets in Reus (Spain) were processed to ensure microbiological safety. Microbiological criteria and procedures have been those included in the European standards for Campylobacter (Regulation No. 2017/1495) and Yersinia (ISO 10273:2917). Results showed the absence of Campylobacter spp. in all samples, but high counts (104 to 5 × 105 CFU/g) of typical colonies (“red bull's eye” morphology) on CIN medium compatible with Yersinia spp. The biochemical profile of the strains from the typical colonies, and their subsequent molecular identification using MALDI-TOF MS enabled us to identify Yersinia intermedia in just one sample, and Pseudomonas and Serratia liquefaciens in the remaining ones. These findings call into question the usefulness of CIN medium for detecting Yersinia spp. in food. Moreover, the presence of high counts of psychrotolerant bacteria from the genera Pseudomonas and Serratia highlight the need to improve hygienic conditions in the procedures used to produce meat derivatives.
{"title":"Detection of Campylobacter spp. and Yersina spp. in chicken burgers: Critical evaluation of CIN medium and characterization of psychrotolerant microbiota in commercial samples from Reus (Tarragona, Spain)","authors":"Marta Sanchis , Laura Camuña-Pardo , Laia Martínez Quijada , Altea Pérez Escobar , Frederic Gómez-Bertomeu , Alberto Miguel Stchigel Glikman","doi":"10.1016/j.ram.2025.06.002","DOIUrl":"10.1016/j.ram.2025.06.002","url":null,"abstract":"<div><div>Two of the three most prevalent bacterial genera associated with human diseases caused by meat consumption are <em>Campylobacter</em> (mainly in poultry meat) and <em>Yersinia</em> (in pork meat). As its detection and quantification is not regulated by the European legislation (except for the quantification of <em>Campylobacter</em> in poultry channels), several samples of chicken burgers from different establishments including supermarkets and retail trade outlets in Reus (Spain) were processed to ensure microbiological safety. Microbiological criteria and procedures have been those included in the European standards for <em>Campylobacter</em> (Regulation No. 2017/1495) and <em>Yersinia</em> (ISO 10273:2917). Results showed the absence of <em>Campylobacter</em> spp. in all samples, but high counts (10<sup>4</sup> to 5<!--> <!-->×<!--> <!-->10<sup>5</sup> <!-->CFU/g) of typical colonies (“red bull's eye” morphology) on CIN medium compatible with <em>Yersinia</em> spp. The biochemical profile of the strains from the typical colonies, and their subsequent molecular identification using MALDI-TOF MS enabled us to identify <em>Yersinia intermedia</em> in just one sample, and <em>Pseudomonas</em> and <em>Serratia liquefaciens</em> in the remaining ones. These findings call into question the usefulness of CIN medium for detecting <em>Yersinia</em> spp. in food. Moreover, the presence of high counts of psychrotolerant bacteria from the genera <em>Pseudomonas</em> and <em>Serratia</em> highlight the need to improve hygienic conditions in the procedures used to produce meat derivatives.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 391-398"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.ram.2024.12.004
María Cecilia Mestre , Mariana E. Langenheim , María Elena Severino , Sonia Fontenla
Plant growth promoting microorganisms play a substantial role in current plant production practices. Yeasts have shown a promising role in enhancing plant production. We studied the production of indole 3-acetic acid (IAA)-like compounds in three psychrotolerant Patagonian native yeast strains. The yeast Tausonia pullulans CRUB 1772, Candida saitoana CRUB 1770 and Saccharomyces eubayanus CRUB 2014 were evaluated using the Salkowski's colorimetric technique and high-performance liquid chromatography (HPLC). T. pullulans CRUB 1772 was the highest producer of IAA-like compounds using the colorimetric technique. Nevertheless, none of the tested yeasts reached the production level of the bacterial strain A. argentinense Az39 used as positive control. The HPLC analysis revealed that only C. saitoana CRUB 1770 and S. eubayanus CRUB 2014 were able to truly produce IAA. Other peaks were also observed, which might correspond to intermediate compounds within the IAA biosynthetic pathway.
{"title":"Do psychrotolerant Patagonian soil yeasts produce 3-indole acetic acid?","authors":"María Cecilia Mestre , Mariana E. Langenheim , María Elena Severino , Sonia Fontenla","doi":"10.1016/j.ram.2024.12.004","DOIUrl":"10.1016/j.ram.2024.12.004","url":null,"abstract":"<div><div>Plant growth promoting microorganisms play a substantial role in current plant production practices. Yeasts have shown a promising role in enhancing plant production. We studied the production of indole 3-acetic acid (IAA)-like compounds in three psychrotolerant Patagonian native yeast strains. The yeast <em>Tausonia pullulans</em> CRUB 1772, <em>Candida saitoana</em> CRUB 1770 and <em>Saccharomyces eubayanus</em> CRUB 2014 were evaluated using the Salkowski's colorimetric technique and high-performance liquid chromatography (HPLC). <em>T. pullulans</em> CRUB 1772 was the highest producer of IAA-like compounds using the colorimetric technique. Nevertheless, none of the tested yeasts reached the production level of the bacterial strain <em>A. argentinense</em> Az39 used as positive control. The HPLC analysis revealed that only <em>C. saitoana</em> CRUB 1770 and <em>S. eubayanus</em> CRUB 2014 were able to truly produce IAA. Other peaks were also observed, which might correspond to intermediate compounds within the IAA biosynthetic pathway.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 371-374"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.ram.2025.03.003
Melisa P. Pérez , Graciela B. Benintende , Diego H. Sauka
This study addresses the pervasive challenge of lesser mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) infestations in poultry farming. Our aim was to select toxic Bacillus thuringiensis strains against A. diaperinus larvae and determine the fraction(s) within the bacterial cultures harboring the active metabolites responsible for this insecticidal activity. Among the 41 strains evaluated, the Argentine strain INTA Mo4-4 showed the highest toxicity. Bioassays revealed that the main virulence factors reside within the spore-crystal pellet associated with the Cry protein. These results set the basis for the development of a targeted bioinsecticide. Further research is needed to elucidate the underlying mechanisms and validate these strategies in field trials. By harnessing B. thuringiensis-based bioinsecticides, we offer a sustainable solution to mitigate A. diaperinus infestations.
{"title":"Toxicity assessment of Bacillus thuringiensis strains for the control of the lesser mealworm beetle Alphitobius diaperinus (Coleoptera: Tenebrionidae)","authors":"Melisa P. Pérez , Graciela B. Benintende , Diego H. Sauka","doi":"10.1016/j.ram.2025.03.003","DOIUrl":"10.1016/j.ram.2025.03.003","url":null,"abstract":"<div><div>This study addresses the pervasive challenge of lesser mealworm <em>Alphitobius diaperinus</em> (Coleoptera: Tenebrionidae) infestations in poultry farming. Our aim was to select toxic <em>Bacillus thuringiensis</em> strains against <em>A. diaperinus</em> larvae and determine the fraction(s) within the bacterial cultures harboring the active metabolites responsible for this insecticidal activity. Among the 41 strains evaluated, the Argentine strain INTA Mo4-4 showed the highest toxicity. Bioassays revealed that the main virulence factors reside within the spore-crystal pellet associated with the Cry protein. These results set the basis for the development of a targeted bioinsecticide. Further research is needed to elucidate the underlying mechanisms and validate these strategies in field trials. By harnessing <em>B. thuringiensis</em>-based bioinsecticides, we offer a sustainable solution to mitigate <em>A. diaperinus</em> infestations.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 375-379"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the last few decades, the emergence of several infectious diseases has increased. Mosquitoes are the most important vectors due to their role in transmitting several pathogens, such as dengue, chikungunya and Plasmodium spp. Recently, mosquitoes have been suspected of transmitting Rickettsias, another emerging pathogen. However, their role in the transmission of this agent remains unclear. In this study, we report the detection of a Rickettsia spp. in Coquillettidia venezuelensis mosquitoes collected from Corrientes Province, Argentina. The phylogenetic analyses show that this Rickettsia is related to Rickettsia felis, and the ompB sequence shows high homology with Rickettsia tillamookensis. The evidence of the detection of Rickettsia in mosquitoes shows that several infectious diseases could be transmitted by mosquitoes, and the interaction between them is important to investigate. More studies should be done in order to analyze the impact on public health.
{"title":"Mosquito-borne pathogens … Now Rickettsias?","authors":"Silvina Goenaga , Estefania Raquel Boaglio , Lucia Victoria Martinez , Juliana Patricia Sanchez","doi":"10.1016/j.ram.2025.03.002","DOIUrl":"10.1016/j.ram.2025.03.002","url":null,"abstract":"<div><div>In the last few decades, the emergence of several infectious diseases has increased. Mosquitoes are the most important vectors due to their role in transmitting several pathogens, such as <em>dengue, chikungunya</em> and <em>Plasmodium</em> spp. Recently, mosquitoes have been suspected of transmitting Rickettsias, another emerging pathogen. However, their role in the transmission of this agent remains unclear. In this study, we report the detection of a <em>Rickettsia</em> spp. in <em>Coquillettidia venezuelensis</em> mosquitoes collected from Corrientes Province, Argentina. The phylogenetic analyses show that this <em>Rickettsia</em> is related to <em>Rickettsia felis</em>, and the <em>ompB</em> sequence shows high homology with <em>Rickettsia tillamookensis.</em> The evidence of the detection of <em>Rickettsia</em> in mosquitoes shows that several infectious diseases could be transmitted by mosquitoes, and the interaction between them is important to investigate. More studies should be done in order to analyze the impact on public health.</div></div>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 364-370"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.ram.2025.11.001
Germán Ernesto Metz, María Gabriela Echeverría
{"title":"The transmission of viral diseases in wild boar using Rutherford's atomic model","authors":"Germán Ernesto Metz, María Gabriela Echeverría","doi":"10.1016/j.ram.2025.11.001","DOIUrl":"10.1016/j.ram.2025.11.001","url":null,"abstract":"","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":"57 4","pages":"Pages 307-308"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145610448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-09DOI: 10.1016/j.ram.2025.07.006
Alejandro N Etchecopaz, Lucia S Di Ciaccio, Katherine Hermida Alava, Reneé H Fortunato, Adriana Salvat, Alejandro D Nusblat, María L Cuestas
Infections caused by the genus Candida have acquired considerable significance in recent years due to the enhanced susceptibility of immunocompromised hosts. There have been increasing reports of multidrug resistance (MDR) in several Candida species, posing a major hurdle to antifungal therapy. Accordingly, exploring and developing novel anti-Candida agents has become a priority. In this study, we assessed the antifungal activity of seven methanolic extracts from the Argentinian native plants Peltophorum dubium, Schinus areira, Parastrephia quadrangularis and Lantana balansae against clinical isolates of different wild-type and MDR isolates of Candida. Synergism with fluconazole was also evaluated. All plant extracts showed antifungal activities against different Candida species, including MDR isolates such as C. haemulonii. Highly active extracts from these native plants provide promising sources of compounds for potentiating the antifungal effect of fluconazole. Further investigation of the chemical constituents of the extracts and their cytotoxicity is needed to develop plant-derived anti-Candida agents.
{"title":"Extracts from Argentinian native plants exhibit antifungal activity against multidrug-resistant Candida species.","authors":"Alejandro N Etchecopaz, Lucia S Di Ciaccio, Katherine Hermida Alava, Reneé H Fortunato, Adriana Salvat, Alejandro D Nusblat, María L Cuestas","doi":"10.1016/j.ram.2025.07.006","DOIUrl":"https://doi.org/10.1016/j.ram.2025.07.006","url":null,"abstract":"<p><p>Infections caused by the genus Candida have acquired considerable significance in recent years due to the enhanced susceptibility of immunocompromised hosts. There have been increasing reports of multidrug resistance (MDR) in several Candida species, posing a major hurdle to antifungal therapy. Accordingly, exploring and developing novel anti-Candida agents has become a priority. In this study, we assessed the antifungal activity of seven methanolic extracts from the Argentinian native plants Peltophorum dubium, Schinus areira, Parastrephia quadrangularis and Lantana balansae against clinical isolates of different wild-type and MDR isolates of Candida. Synergism with fluconazole was also evaluated. All plant extracts showed antifungal activities against different Candida species, including MDR isolates such as C. haemulonii. Highly active extracts from these native plants provide promising sources of compounds for potentiating the antifungal effect of fluconazole. Further investigation of the chemical constituents of the extracts and their cytotoxicity is needed to develop plant-derived anti-Candida agents.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thirty consecutive multidrug-resistant non-carbapenemase-producing Pseudomonas aeruginosa clinical isolates from patients admitted at a university hospital were studied. Resistance rates to ceftazidime/avibactam, aztreonam/avibactam, ceftolozane/tazobactam and imipenem/relebactam were 40%, 88%, 3%, and 20%, respectively. Ceftazidime/avibactam reverted ceftazidime resistance in 25% of the isolates, whereas imipenem/relebactam did so in 77% of the imipenem-resistant isolates. The analysis of the resistance patterns observed suggests a high contribution of non-enzymatic mechanisms such as efflux pumps and porin alterations.
{"title":"Antimicrobial resistance profiles in multidrug-resistant Pseudomonas aeruginosa isolates in a university hospital in Argentina. Activity of new antibiotic combinations.","authors":"Carla Alvarez, Juliana Bello, Carlos Vay, Ángela Famiglietti, Marcela Nastro, Hernán Rodríguez","doi":"10.1016/j.ram.2025.07.003","DOIUrl":"https://doi.org/10.1016/j.ram.2025.07.003","url":null,"abstract":"<p><p>Thirty consecutive multidrug-resistant non-carbapenemase-producing Pseudomonas aeruginosa clinical isolates from patients admitted at a university hospital were studied. Resistance rates to ceftazidime/avibactam, aztreonam/avibactam, ceftolozane/tazobactam and imipenem/relebactam were 40%, 88%, 3%, and 20%, respectively. Ceftazidime/avibactam reverted ceftazidime resistance in 25% of the isolates, whereas imipenem/relebactam did so in 77% of the imipenem-resistant isolates. The analysis of the resistance patterns observed suggests a high contribution of non-enzymatic mechanisms such as efflux pumps and porin alterations.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-04DOI: 10.1016/j.ram.2025.07.005
Rony Cotaquispe Nalvarte, Miriam Legua Barrios, Patricia Escajadillo Luján, Edgard De la Cruz Vásquez, Jennifer Toskano Hurtado
The porcine reproductive and respiratory syndrome (PRRS) is an endemic disease in pork-producing regions of the world, and its control remains poor. Rapid identification of PRRSV-1 and PRRSV-2 species is of great importance for molecular epidemiological surveillance of the virus. The objective of this study was the molecular characterization of the ORF5 gene that synthesizes glycosylated protein 5 (GP5) from PRRS virus detected in pig farms in Lima, Perú. One hundred serum samples were used, obtaining 24 PRRSV-2-positive strains confirmed by two molecular techniques. Phylogenetic inference demonstrated the parental relationship with the NADC34 variant, and we achieved a better phylogenetic relationship of the study strains with Peruvian reference isolates in lineage 1 and sublineage 1.5 and pathogenic strains from various parts of the world. Homology analysis showed that strains 25 to 35, 40 to 41, and 43 to 48 obtained high nucleotide homology values of 95-98% and amino acid homology of 92-98%; strains 36, 37, 38, and 39 with high genetic variability with low nucleotide homology values of 83% and amino acid homology of 79%, and strain 42 with homology of 89 and 84% in relation to the NADC34 variant. In conclusion, we demonstrated the effectiveness of the validated in-house primers for identification compared to a commercial kit, in addition to the evolution and dispersion of lineage 1, sublineage 1.5 and the phylogenetic relationship with the NADC34 variant of the PRRSV-2 species in Lima, Perú.
{"title":"[Molecular characterization of the ORF5 (GP5) gene from porcine reproductive and respiratory syndrome virus (PRRSV) detected in pig farms in Lima, Peru].","authors":"Rony Cotaquispe Nalvarte, Miriam Legua Barrios, Patricia Escajadillo Luján, Edgard De la Cruz Vásquez, Jennifer Toskano Hurtado","doi":"10.1016/j.ram.2025.07.005","DOIUrl":"https://doi.org/10.1016/j.ram.2025.07.005","url":null,"abstract":"<p><p>The porcine reproductive and respiratory syndrome (PRRS) is an endemic disease in pork-producing regions of the world, and its control remains poor. Rapid identification of PRRSV-1 and PRRSV-2 species is of great importance for molecular epidemiological surveillance of the virus. The objective of this study was the molecular characterization of the ORF5 gene that synthesizes glycosylated protein 5 (GP5) from PRRS virus detected in pig farms in Lima, Perú. One hundred serum samples were used, obtaining 24 PRRSV-2-positive strains confirmed by two molecular techniques. Phylogenetic inference demonstrated the parental relationship with the NADC34 variant, and we achieved a better phylogenetic relationship of the study strains with Peruvian reference isolates in lineage 1 and sublineage 1.5 and pathogenic strains from various parts of the world. Homology analysis showed that strains 25 to 35, 40 to 41, and 43 to 48 obtained high nucleotide homology values of 95-98% and amino acid homology of 92-98%; strains 36, 37, 38, and 39 with high genetic variability with low nucleotide homology values of 83% and amino acid homology of 79%, and strain 42 with homology of 89 and 84% in relation to the NADC34 variant. In conclusion, we demonstrated the effectiveness of the validated in-house primers for identification compared to a commercial kit, in addition to the evolution and dispersion of lineage 1, sublineage 1.5 and the phylogenetic relationship with the NADC34 variant of the PRRSV-2 species in Lima, Perú.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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