首页 > 最新文献

Revista Argentina de microbiologia最新文献

英文 中文
In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber. 蓝藻新芽胞外产物A1387对黄瓜枯萎病的体外和体内抑菌活性研究
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-12-19 DOI: 10.1016/j.ram.2024.10.011
Bahareh Nowruzi, Hassan Beiranvand

Fusarium wilt of cucumber, caused by the fungus Fusarium oxysporum, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of Neowestiellopsis persica A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of N. persica A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against F. oxysporum under in vitro and in vivo conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of N. persica A1387 cyanobacteria significantly increased the diameter of the F. oxysporum growth inhibition zone under in vitro conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in F. oxysporum-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of N. persica A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber Fusarium wilt disease.

黄瓜枯萎病是由尖孢镰刀菌(Fusarium oxysporum)引起的黄瓜枯萎病,是造成重大经济损失的主要植物病害。化学杀菌剂的广泛使用造成了环境和健康风险。由于人们越来越关注化学杀菌剂的有害影响,寻找安全有效的生物基替代品来控制植物病害是非常重要的。本研究探讨了新镰刀菌A1387蓝藻代谢物作为化学杀菌剂防治新镰刀菌病的潜力。在体外和体内条件下,研究了核桃A1387蓝藻胞外多糖(EPS)提取物对尖孢镰刀菌的抑菌活性。用蓝细菌EPS提取物处理感染真菌的黄瓜植株,然后评估病害严重程度、抗氧化酶活性和生长参数。在体外条件下,核桃A1387蓝藻生物量和EPS提取物均显著增加了尖孢镰刀菌生长抑制带的直径。用蓝细菌EPS提取物处理导致茎和根的干重和鲜重增加,并显著降低了尖孢镰刀菌感染植物的疾病严重程度和百分比。蓝藻EPS提取物处理真菌感染植株的过氧化物酶、超氧化物歧化酶(SOD)和过氧化氢酶活性在感染第42天显著低于未处理和感染对照植株。研究结果表明,蓝细菌提取物可作为化学杀菌剂的天然安全替代品,有效地防治黄瓜枯萎病。
{"title":"In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber.","authors":"Bahareh Nowruzi, Hassan Beiranvand","doi":"10.1016/j.ram.2024.10.011","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.011","url":null,"abstract":"<p><p>Fusarium wilt of cucumber, caused by the fungus Fusarium oxysporum, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of Neowestiellopsis persica A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of N. persica A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against F. oxysporum under in vitro and in vivo conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of N. persica A1387 cyanobacteria significantly increased the diameter of the F. oxysporum growth inhibition zone under in vitro conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in F. oxysporum-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of N. persica A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber Fusarium wilt disease.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Broaden properties of ambroxol hydrochloride as an antibiofilm compound. 盐酸氨溴索抗生物膜化合物的拓宽性质。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-12-17 DOI: 10.1016/j.ram.2024.10.010
María J González, Michela Lain, Victoria Iribarnegaray, Luciana Robino, Paola Scavone

Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major P. mirabilis efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect P. mirabilis efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.

生物膜相关微生物可引起许多感染,并且是对几种抗菌素产生耐药性的重要原因。抗生素危机导致迫切需要新的治疗工具。氨溴索常用于黏液增多的呼吸道疾病的解黏液剂。此外,还描述了广泛的特性,包括对生物膜的影响。在这项工作中,我们评估了氨溴索对四种具有临床意义的菌株的抗生物膜作用:奇异变形杆菌、大肠杆菌、金黄色葡萄球菌和鲍曼不动杆菌。在体外,用结晶紫定量技术在微孔板和玻璃盖盖上评估生物膜的形成。初步考察了氨溴索对生物膜形成的抑制作用。用预成型生物膜对盐酸氨溴索进行评价,并对活菌/死菌进行定量。分析了氨溴索在溴化乙啶外排试验中的作用,并分析了5个主要奇异假单胞菌外排泵家族基因的相对表达。氨溴索抑制所有细菌的生物膜形成。此外,氨溴索显著降低生物膜生物量和活菌。氨溴索能够根据使用的浓度影响P. mirabilis外排泵,并诱导几个外排泵基因的过表达。总之,氨溴索杀死浮游细胞,减少生物膜生物量,因为它增加细胞死亡,并影响外排泵的表达。此外,它为单独或与抗生素治疗联合治疗生物膜感染提供了可行的替代方案。
{"title":"Broaden properties of ambroxol hydrochloride as an antibiofilm compound.","authors":"María J González, Michela Lain, Victoria Iribarnegaray, Luciana Robino, Paola Scavone","doi":"10.1016/j.ram.2024.10.010","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.010","url":null,"abstract":"<p><p>Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major P. mirabilis efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect P. mirabilis efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential use of Apis mellifera L. honey in the management of the cucurbit powdery mildew caused by Podosphaera xanthii (Castagne) under greenhouse conditions. 蜜蜂蜂蜜在温室条件下治理瓜白粉病的潜在应用
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-12-17 DOI: 10.1016/j.ram.2024.10.007
Juan Antonio Castro-Diego, Carlos Alfonso López-Orona, Verónica Delgado-Pacheco, Miguel Armando López-Beltrán, Nancy Ley-López, Walter Arturo Rubio-Aragón, Jorge Alberto Edeza-Urías

Powdery mildew by Podosphaera xanthii (Castagne) is a major disease of greenhouse cucurbitaceous crops worldwide. Honey by honeybees has been reported as an antimicrobial for diseases in humans, animals, and plants. The aim of this study was to assess Apis mellifera honey against P. xanthii in cucumber plants. During nine consecutive weeks, four different honey concentrations (2.0%, 2.5%, 3.0% and 3.5%), a chemical control (Azoxystrobin) and an untreated check (water) were evaluated. Except for honey at 2%, every concentration was significantly different from the untreated check. Honey concentrations at 3% and 3.5% were found to be the most effective, and their area under disease progress curve (AUDP) was statistically comparable to that of Azoxystrobin with 1048.3, 642.3 and 575.8 AUDP, representing 72.4%, 83.1% and 84.8% of efficiency compared to the untreated check, respectively. These results provide preliminary information on the potential use of honey in managing strategies of the disease under greenhouse conditions.

白粉病(Podosphaera xanthii, Castagne)是世界范围内温室葫芦类作物的主要病害。据报道,蜜蜂的蜂蜜对人类、动物和植物的疾病具有抗菌作用。本研究的目的是评价蜜蜂蜂蜜对黄瓜植株上黄氏单抗的抗性。连续9周,对4种不同蜂蜜浓度(2.0%、2.5%、3.0%和3.5%)、化学对照(偶氮嘧菌酯)和未处理对照(水)进行评价。除了蜂蜜浓度为2%外,每个浓度都与未处理的检查有显著差异。蜂蜜浓度为3%和3.5%时最有效,其疾病进展曲线下面积(AUDP)与偶氮菌酯(1048.3、642.3和575.8 AUDP)具有统计学意义相当,分别比未处理的效率提高72.4%、83.1%和84.8%。这些结果为在温室条件下蜂蜜在病害管理策略中的潜在应用提供了初步信息。
{"title":"Potential use of Apis mellifera L. honey in the management of the cucurbit powdery mildew caused by Podosphaera xanthii (Castagne) under greenhouse conditions.","authors":"Juan Antonio Castro-Diego, Carlos Alfonso López-Orona, Verónica Delgado-Pacheco, Miguel Armando López-Beltrán, Nancy Ley-López, Walter Arturo Rubio-Aragón, Jorge Alberto Edeza-Urías","doi":"10.1016/j.ram.2024.10.007","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.007","url":null,"abstract":"<p><p>Powdery mildew by Podosphaera xanthii (Castagne) is a major disease of greenhouse cucurbitaceous crops worldwide. Honey by honeybees has been reported as an antimicrobial for diseases in humans, animals, and plants. The aim of this study was to assess Apis mellifera honey against P. xanthii in cucumber plants. During nine consecutive weeks, four different honey concentrations (2.0%, 2.5%, 3.0% and 3.5%), a chemical control (Azoxystrobin) and an untreated check (water) were evaluated. Except for honey at 2%, every concentration was significantly different from the untreated check. Honey concentrations at 3% and 3.5% were found to be the most effective, and their area under disease progress curve (AUDP) was statistically comparable to that of Azoxystrobin with 1048.3, 642.3 and 575.8 AUDP, representing 72.4%, 83.1% and 84.8% of efficiency compared to the untreated check, respectively. These results provide preliminary information on the potential use of honey in managing strategies of the disease under greenhouse conditions.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of gestational diabetes mellitus in gut and human breast milk microbiome in Colombian women and their infants. 妊娠期糖尿病对哥伦比亚妇女及其婴儿肠道和母乳微生物组的影响。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-12-17 DOI: 10.1016/j.ram.2024.10.006
Sandra Y Valencia-Castillo, Mayte J Hernández-Beza, Irisbeth Powell-Cerda, Erika Acosta-Cruz, Guadalupe C Rodríguez-Castillejos, Fernando Siller-López, Humberto Martínez-Montoya

Human breast milk (HBM) is a vital source of macronutrients and micronutrients that are crucial for an infant's development. Recent studies have shown that HBM contains diverse microorganisms, including bacteria, viruses, protozoa, and anaerobic fungi. Additionally, novel research has revealed that individuals with metabolic disorders, such as diabetes mellitus, are prone to dysbiosis in their gut microbiome. Our study aimed to investigate the impact of gestational diabetes mellitus (GDM) on HBM and the pair mother-infant gut microbiota. We conducted a comprehensive analysis of two groups from Pereira, Colombia: a GDM group and a non-GDM group. Each group consisted of five infants and their mothers. HBM and stool samples were collected from GDM and non-GDM mother-infant pairs. DNA was purified, and the 16S V3-V4 region was amplified and sequenced. Reads obtained were quality filtered and classified by homology according to the Ribosomal Small Subunit SILVA database. We found significant differences in the relative abundances of gut bacteria between GDM and non-GDM groups. Notably, Bifidobacterium, Serratia and Sutterella were negatively associated in women's gut with GDM. In HBM, Sutterella, Serratia and Lactococcus were found in low RA in the GDM group. Moreover, in the infants, Bifidobacterium, Lactobacillus, Sutterella, Serratia, Streptococcus, and Veillonella had a low presence in GDM. Our findings indicate that there are variations in gut bacteriome profiles between healthy women and those with GDM. These variations may impact the bacterial diversity in HBM, potentially leading to gut bacterial dysbiosis in their infants.

母乳是对婴儿发育至关重要的大量营养素和微量营养素的重要来源。最近的研究表明,HBM含有多种微生物,包括细菌、病毒、原生动物和厌氧真菌。此外,新的研究表明,患有代谢紊乱(如糖尿病)的个体,其肠道微生物群容易发生生态失调。本研究旨在探讨妊娠期糖尿病(GDM)对HBM和母婴肠道菌群的影响。我们对来自哥伦比亚Pereira的两组患者进行了综合分析:GDM组和非GDM组。每组由5名婴儿和他们的母亲组成。收集GDM和非GDM母婴对的HBM和粪便样本。纯化DNA,扩增16S V3-V4区并测序。根据核糖体小亚单位SILVA数据库对获得的Reads进行质量过滤和同源性分类。我们发现GDM组和非GDM组之间肠道细菌的相对丰度存在显著差异。值得注意的是,双歧杆菌、沙雷氏菌和萨特菌在女性肠道中与GDM呈负相关。在HBM中,GDM组低RA中发现沙氏菌、沙雷菌和乳球菌。此外,在婴儿中,双歧杆菌、乳酸菌、沙氏菌、沙雷菌、链球菌和细孔菌在GDM中的存在率较低。我们的研究结果表明,健康女性和GDM患者之间的肠道细菌群存在差异。这些变化可能影响HBM中的细菌多样性,可能导致婴儿肠道细菌生态失调。
{"title":"Impact of gestational diabetes mellitus in gut and human breast milk microbiome in Colombian women and their infants.","authors":"Sandra Y Valencia-Castillo, Mayte J Hernández-Beza, Irisbeth Powell-Cerda, Erika Acosta-Cruz, Guadalupe C Rodríguez-Castillejos, Fernando Siller-López, Humberto Martínez-Montoya","doi":"10.1016/j.ram.2024.10.006","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.006","url":null,"abstract":"<p><p>Human breast milk (HBM) is a vital source of macronutrients and micronutrients that are crucial for an infant's development. Recent studies have shown that HBM contains diverse microorganisms, including bacteria, viruses, protozoa, and anaerobic fungi. Additionally, novel research has revealed that individuals with metabolic disorders, such as diabetes mellitus, are prone to dysbiosis in their gut microbiome. Our study aimed to investigate the impact of gestational diabetes mellitus (GDM) on HBM and the pair mother-infant gut microbiota. We conducted a comprehensive analysis of two groups from Pereira, Colombia: a GDM group and a non-GDM group. Each group consisted of five infants and their mothers. HBM and stool samples were collected from GDM and non-GDM mother-infant pairs. DNA was purified, and the 16S V3-V4 region was amplified and sequenced. Reads obtained were quality filtered and classified by homology according to the Ribosomal Small Subunit SILVA database. We found significant differences in the relative abundances of gut bacteria between GDM and non-GDM groups. Notably, Bifidobacterium, Serratia and Sutterella were negatively associated in women's gut with GDM. In HBM, Sutterella, Serratia and Lactococcus were found in low RA in the GDM group. Moreover, in the infants, Bifidobacterium, Lactobacillus, Sutterella, Serratia, Streptococcus, and Veillonella had a low presence in GDM. Our findings indicate that there are variations in gut bacteriome profiles between healthy women and those with GDM. These variations may impact the bacterial diversity in HBM, potentially leading to gut bacterial dysbiosis in their infants.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula. 尤卡坦半岛北部海岸水和沉积物中的细菌丰富度评估。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-12-05 DOI: 10.1016/j.ram.2024.10.009
Mariela Beatriz Reyes-Sosa, Raúl Valle-Gough, María Del Carmen Ponce-Caballero, María Leticia Arena-Ortiz

Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3-8% of the total sequence reads belonged to opportunistic genera such as: Vibrio in the sea samples and Capnocytophaga in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.

考虑到沿海环境的重要性及其提供的多种生态服务,探索和理解发生在沿海环境中的相互作用是很重要的。微生物组是了解这些脆弱部位动态的关键因素。为了评估尤卡坦半岛北部沿海地区细菌多样性,开展了一项基于16S核糖体基因谱分析的宏基因组研究。结果表明,水体和沉积物样品中所发现的细菌属有一定的相似性,只是数量部分不同。通过主坐标分析,可以观察到沉积物和水样之间的明显差异。湿地和泻湖沉积物样品的相对多样性最高。结果表明,3-8%的总序列读数属于机会性属,如:海洋样品中的弧菌和其他环境中的碳噬菌。盐度和pH值是造成海岸带不同环境下群落差异的主要因素。在研究的海岸带内,不同环境的沉积物具有重要的相似性。本文提供的数据有助于为尤卡坦半岛沿海地区的研究设定基线。
{"title":"Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula.","authors":"Mariela Beatriz Reyes-Sosa, Raúl Valle-Gough, María Del Carmen Ponce-Caballero, María Leticia Arena-Ortiz","doi":"10.1016/j.ram.2024.10.009","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.009","url":null,"abstract":"<p><p>Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3-8% of the total sequence reads belonged to opportunistic genera such as: Vibrio in the sea samples and Capnocytophaga in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center. 粪便分子板在住院腹泻患者中的应用。有三级医疗中心工作经验。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-12-05 DOI: 10.1016/j.ram.2024.10.005
Nicolás Cimmino, Maria Josefina Etchevers, Astrid Smud, Patricia Yolleth Andrade Pino, Marisa Del Lujan Sanchez, Maria Laura Gonzalez, Maria Victoria Acosta, Gissel Zuñiga, Karina Alejandra Perez, Juana Freggiaro, Valeria Rosa Alexander, Maria Florencia Veiga, Mariangeles Visus, Mariano Martin Marcolongo

Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n=41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n=46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.

感染性腹泻是一种常见的健康问题,每年都会影响很多人。它会导致严重的发病率和死亡率,极大地影响医疗保健系统的成本。快速发现病原体和及时治疗可以改变病情的处理和结果。粪便中的分子板可以快速方便地分析多种病原体。本研究采用横断面队列分析,对腹泻住院的成人患者进行回顾性分析,常规粪便细菌学检查阴性。分析了从使用粪便分子板获得的数据和内窥镜在诊断途径中的作用。79份样本的阳性率为52% (n=41)。该试验改变了58% (n=46)患者的治疗方法。在分子检查结果为阴性的患者中,39.5%的患者接受了结肠镜检查和活检的进一步评估,诊断率为87%。基于这些结果,我们可以得出结论,分子技术有助于腹泻住院患者的诊断和治疗方法的改变。
{"title":"Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center.","authors":"Nicolás Cimmino, Maria Josefina Etchevers, Astrid Smud, Patricia Yolleth Andrade Pino, Marisa Del Lujan Sanchez, Maria Laura Gonzalez, Maria Victoria Acosta, Gissel Zuñiga, Karina Alejandra Perez, Juana Freggiaro, Valeria Rosa Alexander, Maria Florencia Veiga, Mariangeles Visus, Mariano Martin Marcolongo","doi":"10.1016/j.ram.2024.10.005","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.005","url":null,"abstract":"<p><p>Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n=41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n=46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Risk factors associated with the presence of brucellosis in mixed establishments (caprine and bovine) in the province of Formosa, Argentina]. [阿根廷福莫萨省混合饲养场(山羊和牛)出现布鲁氏菌病的相关风险因素]。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-11-20 DOI: 10.1016/j.ram.2024.10.003
Iris Carolina Elías, María Nair Viola, Ana Maria Russo, Marcelo Signorini Porchietto

Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (odds ratio, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.

布鲁氏菌病是一种分布于世界各地的人畜共患疾病,会造成严重的生产损失,并对公众健康产生负面影响。这项工作旨在确定与福莫萨省中西部地区混合养殖场(牛/羊)中出现布鲁氏菌病相关的风险因素。在 2022/2023 年期间,对 67 个混合农场的 7855 头牲畜(2943 头牛和 4912 只山羊)进行了随机抽样。对样本进行血清学分析(BPA + FPA),以确定哪些动物呈阳性。同样,还对每个农场进行了保密调查,以评估潜在的相关变量。统计分析采用广义线性混合模型(GLMM),二项分布,对数连接函数,农场为随机变量。混合养殖场的布鲁氏菌病流行率估计为 9%,而每头动物(牛和山羊)的联合流行率为 2.75%。牛和山羊布鲁氏菌病流行的原因包括:动物出现生殖症状(几率比,OR=40)、妊娠末期发生流产和产下弱小后代(OR=5.3)、流产处理不当(OR=8)、从其他农场引进动物(OR=5.9)和没有阴性布鲁氏菌病证明(OR=9.6)。每个猪场的管理措施,如预防措施、圈舍卫生措施和工人预防措施,对控制布鲁氏菌病非常重要。
{"title":"[Risk factors associated with the presence of brucellosis in mixed establishments (caprine and bovine) in the province of Formosa, Argentina].","authors":"Iris Carolina Elías, María Nair Viola, Ana Maria Russo, Marcelo Signorini Porchietto","doi":"10.1016/j.ram.2024.10.003","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.003","url":null,"abstract":"<p><p>Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (odds ratio, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Bartholinitis due to Neisseria meningitidis: Clinical case]. [脑膜炎奈瑟菌引起的巴氏腺炎:临床病例]。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-11-19 DOI: 10.1016/j.ram.2024.10.004
Elena Berardinelli, Marcela Sokol, Laura Dufranc, Vanina Diaz, Viviana Eiras, Ariel Giancieni, Patricia Galarza, Paula Gagetti, Federico Lorenzo, Adriana Efron

Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by Neisseria meningitidis, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of N. meningitidis as the pathogen responsible for bartholinitis.

巴氏腺炎是指巴氏腺导管内粘液积聚导致的炎症和感染,最常见的致病微生物是厌氧菌、需氧菌和性传播感染的病原体。由不属于生殖器微生物群的病原体引起的病例较少。同样,大多数情况下也是通过临床诊断。在此,我们描述了一名 42 岁异性恋女性的病例,她的巴氏腺脓肿是由脑膜炎奈瑟菌引起的,通过培养分离并经 VITEK®2 鉴定。在分子特征和亚型鉴定方面,采用多位点序列分型技术确定了序列类型和克隆复合体。通过对 PorA、fHbp、NHBA 和 NadA 的编码基因进行扩增和测序,研究了抗原谱,并通过 MIC 评估了药敏谱。通过分子诊断,确认脑膜炎双球菌是引起巴氏脑炎的病原体。
{"title":"[Bartholinitis due to Neisseria meningitidis: Clinical case].","authors":"Elena Berardinelli, Marcela Sokol, Laura Dufranc, Vanina Diaz, Viviana Eiras, Ariel Giancieni, Patricia Galarza, Paula Gagetti, Federico Lorenzo, Adriana Efron","doi":"10.1016/j.ram.2024.10.004","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.004","url":null,"abstract":"<p><p>Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by Neisseria meningitidis, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of N. meningitidis as the pathogen responsible for bartholinitis.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Atypical non-Typhi Salmonella enterica bacteremia, lactose-positive, lysine decarboxylase-negative]. [非典型非Typhi肠炎沙门氏菌菌血症,乳糖阳性,赖氨酸脱羧酶阴性]。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-11-16 DOI: 10.1016/j.ram.2024.09.007
Susana D García, María I Caffer, Marisa N Almuzara, Ángela M R Famiglietti, Cristina Myburg, María R Viñas, Jorge Risso, Carlos A Vay

We describe here the first isolation in Argentina of a lactose-fermenting and lysine decarboxylase-negative non-Typhi Salmonella enterica from a blood culture of a patient with systemic lupus erythematosus. The microorganism was identified as S. enterica (score: 2.148) by mass spectrometry, as "Salmonella group" (discrimination: 86%) by the VITEK® 2 system and as Citrobacter freundii complex by biochemical tests. The serological test using the OMA antiserum for the somatic antigen was positive. The identification was confirmed as Salmonella subsp. I 4,[5],12:i:- atypical at the National Reference Center ANLIS Dr. Carlos G. Malbrán. Mass spectrometry is especially useful in microbial identification in the presence of atypia, while biochemical tests can lead to misidentification in these cases. This study warns about the circulation of this strain in Argentina.

我们在此描述了阿根廷首次从一名系统性红斑狼疮患者的血液培养物中分离出一种乳糖发酵和赖氨酸脱羧酶阴性的非泰菲氏肠炎沙门氏菌。该微生物通过质谱鉴定为肠炎沙门氏菌(得分:2.148),通过 VITEK® 2 系统鉴定为 "沙门氏菌群"(辨别率:86%),通过生化检验鉴定为弗氏柠檬酸杆菌复合物。使用体细胞抗原 OMA 抗血清进行的血清学检测呈阳性。卡洛斯-马尔布兰博士(Carlos G. Malbrán)在国家参考资料中心(ANLIS Dr. Carlos G. Malbrán)确认其为 I 4[5],12:i:-非典型沙门氏菌亚种。质谱法在非典型性微生物鉴定中特别有用,而生化检验在这些情况下可能会导致错误鉴定。这项研究对这种菌株在阿根廷的传播提出了警告。
{"title":"[Atypical non-Typhi Salmonella enterica bacteremia, lactose-positive, lysine decarboxylase-negative].","authors":"Susana D García, María I Caffer, Marisa N Almuzara, Ángela M R Famiglietti, Cristina Myburg, María R Viñas, Jorge Risso, Carlos A Vay","doi":"10.1016/j.ram.2024.09.007","DOIUrl":"https://doi.org/10.1016/j.ram.2024.09.007","url":null,"abstract":"<p><p>We describe here the first isolation in Argentina of a lactose-fermenting and lysine decarboxylase-negative non-Typhi Salmonella enterica from a blood culture of a patient with systemic lupus erythematosus. The microorganism was identified as S. enterica (score: 2.148) by mass spectrometry, as \"Salmonella group\" (discrimination: 86%) by the VITEK® 2 system and as Citrobacter freundii complex by biochemical tests. The serological test using the OMA antiserum for the somatic antigen was positive. The identification was confirmed as Salmonella subsp. I 4,[5],12:i:- atypical at the National Reference Center ANLIS Dr. Carlos G. Malbrán. Mass spectrometry is especially useful in microbial identification in the presence of atypia, while biochemical tests can lead to misidentification in these cases. This study warns about the circulation of this strain in Argentina.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection and characterization of highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b virus circulating in Argentina in 2023. 2023 年在阿根廷流行的高致病性甲型禽流感(H5N1)2.3.4.4b 支系病毒的检测和特征描述。
IF 1.8 4区 生物学 Q4 MICROBIOLOGY Pub Date : 2024-11-11 DOI: 10.1016/j.ram.2024.08.002
María Carolina Artuso, Vanina Daniela Marchione, Estefanía Benedetti, Paula Bonastre, Ana María Alvarez, Luana Piccini, Angeles Ponde, Evelyn Barrios Benito, Marcos Fabeiro, Karen Waisman, Luciano Coppola, Tomás Poklepovich, Ariana Chamorro, Martín Avaro, Diego Ariel Riva, Andrea Pontoriero, María Eugenia Ferrer, Andrea Marcos, Lorena Dassa, Daniel Caria, Ximena Melon, Rodrigo Emmanuel Balzano Parodi, Ana María Nicola

In 2021, avian influenza A (H5N1) clade 2.3.4.4b virus spread to North America and then to Central and South America in October 2022, extending from Colombia to Chile in three months. During 2023, several countries, mostly in the Americas, reported outbreaks in poultry, wild birds and mammals, as well as the emergence of two cases in humans (one in Ecuador in January and one in Chile in March). As of September 20th, 2023, 17 countries in the Americas Region have recorded cases of A (H5N1) in birds and mammals. On February 14th, 2023, Argentina confirmed the first case of avian influenza in wild birds, which was later detected in backyard and commercial poultry, and in the South-American sea lion (Otaria flavescens) in Tierra del Fuego, in the south of the country. So far, 21 suspected cases have been recorded in humans; however, all of them tested negative for Influenza A virus. Hemagglutinin sequence data of animal viruses analyzed in this report showed that Argentinian viruses clustered together with those isolated in other countries of the region. Epidemiological data suggested the possibility of multiple simultaneous entries of the avian virus, highlighting the role of migratory avian populations in the introduction and dissemination of the disease in Argentina. Continued comprehensive surveillance of these viruses in animals and people worldwide, along with ongoing preparedness efforts, are critical to determine the public health risk.

2021 年,甲型禽流感(H5N1)2.3.4.4b 支系病毒扩散到北美洲,然后于 2022 年 10 月扩散到中美洲和南美洲,在三个月内从哥伦比亚扩展到智利。2023 年期间,多个国家(主要是美洲国家)报告在家禽、野鸟和哺乳动物中爆发疫情,并出现两例人类病例(一例于 1 月在厄瓜多尔,一例于 3 月在智利)。截至 2023 年 9 月 20 日,美洲地区已有 17 个国家记录到禽类和哺乳动物感染甲型 (H5N1) 流感病例。2023 年 2 月 14 日,阿根廷确诊首例野鸟感染禽流感病例,随后在散养家禽和商业家禽以及该国南部火地岛的南美海狮(Otaria flavescens)中发现该病例。迄今为止,已记录了 21 例人类疑似病例,但所有病例的甲型流感病毒检测结果均为阴性。本报告分析的动物病毒血凝素序列数据显示,阿根廷的病毒与该地区其他国家分离出的病毒聚集在一起。流行病学数据表明,禽流感病毒有可能同时进入多个国家,这凸显了候鸟种群在阿根廷引入和传播该疾病中的作用。继续在全球范围内对动物和人体内的这些病毒进行全面监测,同时持续开展防备工作,对于确定公共卫生风险至关重要。
{"title":"Detection and characterization of highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b virus circulating in Argentina in 2023.","authors":"María Carolina Artuso, Vanina Daniela Marchione, Estefanía Benedetti, Paula Bonastre, Ana María Alvarez, Luana Piccini, Angeles Ponde, Evelyn Barrios Benito, Marcos Fabeiro, Karen Waisman, Luciano Coppola, Tomás Poklepovich, Ariana Chamorro, Martín Avaro, Diego Ariel Riva, Andrea Pontoriero, María Eugenia Ferrer, Andrea Marcos, Lorena Dassa, Daniel Caria, Ximena Melon, Rodrigo Emmanuel Balzano Parodi, Ana María Nicola","doi":"10.1016/j.ram.2024.08.002","DOIUrl":"https://doi.org/10.1016/j.ram.2024.08.002","url":null,"abstract":"<p><p>In 2021, avian influenza A (H5N1) clade 2.3.4.4b virus spread to North America and then to Central and South America in October 2022, extending from Colombia to Chile in three months. During 2023, several countries, mostly in the Americas, reported outbreaks in poultry, wild birds and mammals, as well as the emergence of two cases in humans (one in Ecuador in January and one in Chile in March). As of September 20th, 2023, 17 countries in the Americas Region have recorded cases of A (H5N1) in birds and mammals. On February 14th, 2023, Argentina confirmed the first case of avian influenza in wild birds, which was later detected in backyard and commercial poultry, and in the South-American sea lion (Otaria flavescens) in Tierra del Fuego, in the south of the country. So far, 21 suspected cases have been recorded in humans; however, all of them tested negative for Influenza A virus. Hemagglutinin sequence data of animal viruses analyzed in this report showed that Argentinian viruses clustered together with those isolated in other countries of the region. Epidemiological data suggested the possibility of multiple simultaneous entries of the avian virus, highlighting the role of migratory avian populations in the introduction and dissemination of the disease in Argentina. Continued comprehensive surveillance of these viruses in animals and people worldwide, along with ongoing preparedness efforts, are critical to determine the public health risk.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Revista Argentina de microbiologia
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1