Pub Date : 2024-12-19DOI: 10.1016/j.ram.2024.10.011
Bahareh Nowruzi, Hassan Beiranvand
Fusarium wilt of cucumber, caused by the fungus Fusarium oxysporum, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of Neowestiellopsis persica A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of N. persica A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against F. oxysporum under in vitro and in vivo conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of N. persica A1387 cyanobacteria significantly increased the diameter of the F. oxysporum growth inhibition zone under in vitro conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in F. oxysporum-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of N. persica A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber Fusarium wilt disease.
{"title":"In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber.","authors":"Bahareh Nowruzi, Hassan Beiranvand","doi":"10.1016/j.ram.2024.10.011","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.011","url":null,"abstract":"<p><p>Fusarium wilt of cucumber, caused by the fungus Fusarium oxysporum, is a major plant disease that causes significant economic losses. The extensive use of chemical fungicides for its control poses environmental and health risks. Due to growing concerns about the detrimental effects of chemical fungicides, finding safe and effective bio-based alternatives for plant disease control is of high importance. In this study, the potential of Neowestiellopsis persica A1387 cyanobacterial metabolites as a promising substitute for chemical fungicides in controlling this disease was investigated. The antifungal activity of N. persica A1387 cyanobacterial exopolysaccharide (EPS) extract was evaluated against F. oxysporum under in vitro and in vivo conditions. Cucumber plants infected with the fungus were treated with cyanobacterial EPS extract and then assessed for disease severity, antioxidant enzyme activity, and growth parameters. Both biomass and EPS extracts of N. persica A1387 cyanobacteria significantly increased the diameter of the F. oxysporum growth inhibition zone under in vitro conditions. Treatment with cyanobacterial EPS extract resulted in increased dry and fresh weight of stem and roots, and a significant reduction in disease severity and percentage in F. oxysporum-infected plants. Peroxidase, superoxide dismutase (SOD), and catalase enzyme activities in fungus-infected plants treated with cyanobacterial EPS extract were significantly lower on day 42 of infection compared to untreated and infected control plants. These findings demonstrate the potential of N. persica A1387 cyanobacterial extracts as natural and safe alternatives to chemical fungicides for controlling cucumber Fusarium wilt disease.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-17DOI: 10.1016/j.ram.2024.10.010
María J González, Michela Lain, Victoria Iribarnegaray, Luciana Robino, Paola Scavone
Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major P. mirabilis efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect P. mirabilis efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.
{"title":"Broaden properties of ambroxol hydrochloride as an antibiofilm compound.","authors":"María J González, Michela Lain, Victoria Iribarnegaray, Luciana Robino, Paola Scavone","doi":"10.1016/j.ram.2024.10.010","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.010","url":null,"abstract":"<p><p>Biofilm-associated microorganisms can cause many infections and are an important cause of resistance to several antimicrobials. The antibiotic crisis has led to a pressing need for new therapeutic tools. Ambroxol is frequently used as a mucolytic agent in respiratory diseases with increased mucus production. In addition, a wide range of properties has been described, including the effect on biofilms. In this work, we evaluate the anti-biofilm effect of ambroxol on four strains with clinical relevance: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. In vitro, biofilm formation was assessed using the crystal violet quantification technique in microplate and glass coverslip. The inhibition of biofilm formation was evaluated by adding ambroxol at the initial time. Ambroxol hydrochloride was evaluated over the preformed biofilm and live/dead bacteria were quantified. The effect of ambroxol in the ethidium bromide efflux assay and the relative expression of the five major P. mirabilis efflux pump family genes were analyzed. Ambroxol inhibited biofilm formation in all the bacteria tested. Moreover, ambroxol significantly reduces both biofilm biomass and viable bacteria. Ambroxol was able to affect P. mirabilis efflux pumps depending on the concentration used and induced the overexpression of several efflux pump genes. In summary, ambroxol kills planktonic cells, reduce biofilm biomass as it increases cell death, and affect the expression of efflux pumps. Furthermore, it presents a viable alternative for the treatment of biofilm infection alone or in combination with antibiotic therapy.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-17DOI: 10.1016/j.ram.2024.10.007
Juan Antonio Castro-Diego, Carlos Alfonso López-Orona, Verónica Delgado-Pacheco, Miguel Armando López-Beltrán, Nancy Ley-López, Walter Arturo Rubio-Aragón, Jorge Alberto Edeza-Urías
Powdery mildew by Podosphaera xanthii (Castagne) is a major disease of greenhouse cucurbitaceous crops worldwide. Honey by honeybees has been reported as an antimicrobial for diseases in humans, animals, and plants. The aim of this study was to assess Apis mellifera honey against P. xanthii in cucumber plants. During nine consecutive weeks, four different honey concentrations (2.0%, 2.5%, 3.0% and 3.5%), a chemical control (Azoxystrobin) and an untreated check (water) were evaluated. Except for honey at 2%, every concentration was significantly different from the untreated check. Honey concentrations at 3% and 3.5% were found to be the most effective, and their area under disease progress curve (AUDP) was statistically comparable to that of Azoxystrobin with 1048.3, 642.3 and 575.8 AUDP, representing 72.4%, 83.1% and 84.8% of efficiency compared to the untreated check, respectively. These results provide preliminary information on the potential use of honey in managing strategies of the disease under greenhouse conditions.
{"title":"Potential use of Apis mellifera L. honey in the management of the cucurbit powdery mildew caused by Podosphaera xanthii (Castagne) under greenhouse conditions.","authors":"Juan Antonio Castro-Diego, Carlos Alfonso López-Orona, Verónica Delgado-Pacheco, Miguel Armando López-Beltrán, Nancy Ley-López, Walter Arturo Rubio-Aragón, Jorge Alberto Edeza-Urías","doi":"10.1016/j.ram.2024.10.007","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.007","url":null,"abstract":"<p><p>Powdery mildew by Podosphaera xanthii (Castagne) is a major disease of greenhouse cucurbitaceous crops worldwide. Honey by honeybees has been reported as an antimicrobial for diseases in humans, animals, and plants. The aim of this study was to assess Apis mellifera honey against P. xanthii in cucumber plants. During nine consecutive weeks, four different honey concentrations (2.0%, 2.5%, 3.0% and 3.5%), a chemical control (Azoxystrobin) and an untreated check (water) were evaluated. Except for honey at 2%, every concentration was significantly different from the untreated check. Honey concentrations at 3% and 3.5% were found to be the most effective, and their area under disease progress curve (AUDP) was statistically comparable to that of Azoxystrobin with 1048.3, 642.3 and 575.8 AUDP, representing 72.4%, 83.1% and 84.8% of efficiency compared to the untreated check, respectively. These results provide preliminary information on the potential use of honey in managing strategies of the disease under greenhouse conditions.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-17DOI: 10.1016/j.ram.2024.10.006
Sandra Y Valencia-Castillo, Mayte J Hernández-Beza, Irisbeth Powell-Cerda, Erika Acosta-Cruz, Guadalupe C Rodríguez-Castillejos, Fernando Siller-López, Humberto Martínez-Montoya
Human breast milk (HBM) is a vital source of macronutrients and micronutrients that are crucial for an infant's development. Recent studies have shown that HBM contains diverse microorganisms, including bacteria, viruses, protozoa, and anaerobic fungi. Additionally, novel research has revealed that individuals with metabolic disorders, such as diabetes mellitus, are prone to dysbiosis in their gut microbiome. Our study aimed to investigate the impact of gestational diabetes mellitus (GDM) on HBM and the pair mother-infant gut microbiota. We conducted a comprehensive analysis of two groups from Pereira, Colombia: a GDM group and a non-GDM group. Each group consisted of five infants and their mothers. HBM and stool samples were collected from GDM and non-GDM mother-infant pairs. DNA was purified, and the 16S V3-V4 region was amplified and sequenced. Reads obtained were quality filtered and classified by homology according to the Ribosomal Small Subunit SILVA database. We found significant differences in the relative abundances of gut bacteria between GDM and non-GDM groups. Notably, Bifidobacterium, Serratia and Sutterella were negatively associated in women's gut with GDM. In HBM, Sutterella, Serratia and Lactococcus were found in low RA in the GDM group. Moreover, in the infants, Bifidobacterium, Lactobacillus, Sutterella, Serratia, Streptococcus, and Veillonella had a low presence in GDM. Our findings indicate that there are variations in gut bacteriome profiles between healthy women and those with GDM. These variations may impact the bacterial diversity in HBM, potentially leading to gut bacterial dysbiosis in their infants.
{"title":"Impact of gestational diabetes mellitus in gut and human breast milk microbiome in Colombian women and their infants.","authors":"Sandra Y Valencia-Castillo, Mayte J Hernández-Beza, Irisbeth Powell-Cerda, Erika Acosta-Cruz, Guadalupe C Rodríguez-Castillejos, Fernando Siller-López, Humberto Martínez-Montoya","doi":"10.1016/j.ram.2024.10.006","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.006","url":null,"abstract":"<p><p>Human breast milk (HBM) is a vital source of macronutrients and micronutrients that are crucial for an infant's development. Recent studies have shown that HBM contains diverse microorganisms, including bacteria, viruses, protozoa, and anaerobic fungi. Additionally, novel research has revealed that individuals with metabolic disorders, such as diabetes mellitus, are prone to dysbiosis in their gut microbiome. Our study aimed to investigate the impact of gestational diabetes mellitus (GDM) on HBM and the pair mother-infant gut microbiota. We conducted a comprehensive analysis of two groups from Pereira, Colombia: a GDM group and a non-GDM group. Each group consisted of five infants and their mothers. HBM and stool samples were collected from GDM and non-GDM mother-infant pairs. DNA was purified, and the 16S V3-V4 region was amplified and sequenced. Reads obtained were quality filtered and classified by homology according to the Ribosomal Small Subunit SILVA database. We found significant differences in the relative abundances of gut bacteria between GDM and non-GDM groups. Notably, Bifidobacterium, Serratia and Sutterella were negatively associated in women's gut with GDM. In HBM, Sutterella, Serratia and Lactococcus were found in low RA in the GDM group. Moreover, in the infants, Bifidobacterium, Lactobacillus, Sutterella, Serratia, Streptococcus, and Veillonella had a low presence in GDM. Our findings indicate that there are variations in gut bacteriome profiles between healthy women and those with GDM. These variations may impact the bacterial diversity in HBM, potentially leading to gut bacterial dysbiosis in their infants.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05DOI: 10.1016/j.ram.2024.10.009
Mariela Beatriz Reyes-Sosa, Raúl Valle-Gough, María Del Carmen Ponce-Caballero, María Leticia Arena-Ortiz
Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3-8% of the total sequence reads belonged to opportunistic genera such as: Vibrio in the sea samples and Capnocytophaga in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.
{"title":"Bacterial richness assessment in water and sediments in the northern coast of the Yucatan Peninsula.","authors":"Mariela Beatriz Reyes-Sosa, Raúl Valle-Gough, María Del Carmen Ponce-Caballero, María Leticia Arena-Ortiz","doi":"10.1016/j.ram.2024.10.009","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.009","url":null,"abstract":"<p><p>Given the importance of the coastal environments and the multiple ecological services that they provide, it is important to explore and understand the interactions that occur within them. The microbiome is a key factor for the understanding of the dynamics of these fragile sites. A metagenomic study based on the profiling of the 16S ribosomal gene was carried out in order to assess the bacterial diversity present in the northern coastal zone of the Yucatan Peninsula. The results showed that water and sediment samples share some similarities regarding the bacterial genera found, only differing in the quantitative part. Through a PCO (principal coordinates) analysis clear differences between sediment and water samples could be observed. The highest relative diversity was found in wetland and lagoon sediment samples, respectively. It was observed that 3-8% of the total sequence reads belonged to opportunistic genera such as: Vibrio in the sea samples and Capnocytophaga in the other environments. Salinity and pH were the factors which contributed the most to the differences among the communities in the various environments in the coastal zone. There is an important similarity in the sediments across the different environments within the studied coastal zone. The data presented herein contribute to setting a baseline for research in the coastal region of the Yucatan Peninsula.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05DOI: 10.1016/j.ram.2024.10.005
Nicolás Cimmino, Maria Josefina Etchevers, Astrid Smud, Patricia Yolleth Andrade Pino, Marisa Del Lujan Sanchez, Maria Laura Gonzalez, Maria Victoria Acosta, Gissel Zuñiga, Karina Alejandra Perez, Juana Freggiaro, Valeria Rosa Alexander, Maria Florencia Veiga, Mariangeles Visus, Mariano Martin Marcolongo
Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n=41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n=46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.
{"title":"Use of stool molecular panel in hospitalized patients with diarrhea. Experience in a tertiary care center.","authors":"Nicolás Cimmino, Maria Josefina Etchevers, Astrid Smud, Patricia Yolleth Andrade Pino, Marisa Del Lujan Sanchez, Maria Laura Gonzalez, Maria Victoria Acosta, Gissel Zuñiga, Karina Alejandra Perez, Juana Freggiaro, Valeria Rosa Alexander, Maria Florencia Veiga, Mariangeles Visus, Mariano Martin Marcolongo","doi":"10.1016/j.ram.2024.10.005","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.005","url":null,"abstract":"<p><p>Infectious diarrhea is a common health issue that affects a large number of individuals each year. It causes significant morbidity and mortality, greatly impacting healthcare system costs. Rapid detection of the causative organism and timely treatment alters the management and outcome of the condition. Molecular panels in stool allow to analyze a wide range of pathogens quickly and easily. For this study, a cross-sectional cohort analysis with a retrospective analysis of adult patients hospitalized with diarrhea and negative conventional stool bacteriological studies was conducted. Data obtained from the use of molecular panels in stool and the role of endoscopy in the diagnostic pathway were analyzed. A positivity rate of 52% (n=41) out of a total of 79 samples was reported. The test contributed to a change in therapeutic approach in 58% (n=46) of the patients. Among the patients with a negative molecular panel, 39.5% underwent further evaluation with colonoscopy involving biopsies, resulting in a diagnostic yield of 87%. Based on these results, we can conclude that molecular techniques contribute to the diagnosis and change in therapeutic approach in hospitalized patients with diarrhea.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-20DOI: 10.1016/j.ram.2024.10.003
Iris Carolina Elías, María Nair Viola, Ana Maria Russo, Marcelo Signorini Porchietto
Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (odds ratio, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.
{"title":"[Risk factors associated with the presence of brucellosis in mixed establishments (caprine and bovine) in the province of Formosa, Argentina].","authors":"Iris Carolina Elías, María Nair Viola, Ana Maria Russo, Marcelo Signorini Porchietto","doi":"10.1016/j.ram.2024.10.003","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.003","url":null,"abstract":"<p><p>Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (odds ratio, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-19DOI: 10.1016/j.ram.2024.10.004
Elena Berardinelli, Marcela Sokol, Laura Dufranc, Vanina Diaz, Viviana Eiras, Ariel Giancieni, Patricia Galarza, Paula Gagetti, Federico Lorenzo, Adriana Efron
Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by Neisseria meningitidis, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of N. meningitidis as the pathogen responsible for bartholinitis.
{"title":"[Bartholinitis due to Neisseria meningitidis: Clinical case].","authors":"Elena Berardinelli, Marcela Sokol, Laura Dufranc, Vanina Diaz, Viviana Eiras, Ariel Giancieni, Patricia Galarza, Paula Gagetti, Federico Lorenzo, Adriana Efron","doi":"10.1016/j.ram.2024.10.004","DOIUrl":"https://doi.org/10.1016/j.ram.2024.10.004","url":null,"abstract":"<p><p>Bartholinitis is the inflammation and infection of the Bartholin's glands that results from the accumulation of mucus in their ducts, the most frequent causal microorganisms being anaerobic and aerobic bacteria and those responsible for sexually transmitted infections. Those caused by agents not belonging to the genital microbiota are less frequent. Likewise, in most cases the diagnosis is clinical. Here, we describe the case of a 42-year-old heterosexual woman with a Bartholin's gland abscess caused by Neisseria meningitidis, isolated through culture and identified by VITEK®2. For molecular characterization and subtyping, the sequence type and clonal complex were determined using the multiple locus sequence typing technique. The antigenic profile was studied by amplification and sequencing of the genes coding for PorA, fHbp, NHBA and NadA and the susceptibility profile was assessed by MIC. Molecular diagnosis led to the confirmation of N. meningitidis as the pathogen responsible for bartholinitis.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-16DOI: 10.1016/j.ram.2024.09.007
Susana D García, María I Caffer, Marisa N Almuzara, Ángela M R Famiglietti, Cristina Myburg, María R Viñas, Jorge Risso, Carlos A Vay
We describe here the first isolation in Argentina of a lactose-fermenting and lysine decarboxylase-negative non-Typhi Salmonella enterica from a blood culture of a patient with systemic lupus erythematosus. The microorganism was identified as S. enterica (score: 2.148) by mass spectrometry, as "Salmonella group" (discrimination: 86%) by the VITEK® 2 system and as Citrobacter freundii complex by biochemical tests. The serological test using the OMA antiserum for the somatic antigen was positive. The identification was confirmed as Salmonella subsp. I 4,[5],12:i:- atypical at the National Reference Center ANLIS Dr. Carlos G. Malbrán. Mass spectrometry is especially useful in microbial identification in the presence of atypia, while biochemical tests can lead to misidentification in these cases. This study warns about the circulation of this strain in Argentina.
我们在此描述了阿根廷首次从一名系统性红斑狼疮患者的血液培养物中分离出一种乳糖发酵和赖氨酸脱羧酶阴性的非泰菲氏肠炎沙门氏菌。该微生物通过质谱鉴定为肠炎沙门氏菌(得分:2.148),通过 VITEK® 2 系统鉴定为 "沙门氏菌群"(辨别率:86%),通过生化检验鉴定为弗氏柠檬酸杆菌复合物。使用体细胞抗原 OMA 抗血清进行的血清学检测呈阳性。卡洛斯-马尔布兰博士(Carlos G. Malbrán)在国家参考资料中心(ANLIS Dr. Carlos G. Malbrán)确认其为 I 4[5],12:i:-非典型沙门氏菌亚种。质谱法在非典型性微生物鉴定中特别有用,而生化检验在这些情况下可能会导致错误鉴定。这项研究对这种菌株在阿根廷的传播提出了警告。
{"title":"[Atypical non-Typhi Salmonella enterica bacteremia, lactose-positive, lysine decarboxylase-negative].","authors":"Susana D García, María I Caffer, Marisa N Almuzara, Ángela M R Famiglietti, Cristina Myburg, María R Viñas, Jorge Risso, Carlos A Vay","doi":"10.1016/j.ram.2024.09.007","DOIUrl":"https://doi.org/10.1016/j.ram.2024.09.007","url":null,"abstract":"<p><p>We describe here the first isolation in Argentina of a lactose-fermenting and lysine decarboxylase-negative non-Typhi Salmonella enterica from a blood culture of a patient with systemic lupus erythematosus. The microorganism was identified as S. enterica (score: 2.148) by mass spectrometry, as \"Salmonella group\" (discrimination: 86%) by the VITEK® 2 system and as Citrobacter freundii complex by biochemical tests. The serological test using the OMA antiserum for the somatic antigen was positive. The identification was confirmed as Salmonella subsp. I 4,[5],12:i:- atypical at the National Reference Center ANLIS Dr. Carlos G. Malbrán. Mass spectrometry is especially useful in microbial identification in the presence of atypia, while biochemical tests can lead to misidentification in these cases. This study warns about the circulation of this strain in Argentina.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-11DOI: 10.1016/j.ram.2024.08.002
María Carolina Artuso, Vanina Daniela Marchione, Estefanía Benedetti, Paula Bonastre, Ana María Alvarez, Luana Piccini, Angeles Ponde, Evelyn Barrios Benito, Marcos Fabeiro, Karen Waisman, Luciano Coppola, Tomás Poklepovich, Ariana Chamorro, Martín Avaro, Diego Ariel Riva, Andrea Pontoriero, María Eugenia Ferrer, Andrea Marcos, Lorena Dassa, Daniel Caria, Ximena Melon, Rodrigo Emmanuel Balzano Parodi, Ana María Nicola
In 2021, avian influenza A (H5N1) clade 2.3.4.4b virus spread to North America and then to Central and South America in October 2022, extending from Colombia to Chile in three months. During 2023, several countries, mostly in the Americas, reported outbreaks in poultry, wild birds and mammals, as well as the emergence of two cases in humans (one in Ecuador in January and one in Chile in March). As of September 20th, 2023, 17 countries in the Americas Region have recorded cases of A (H5N1) in birds and mammals. On February 14th, 2023, Argentina confirmed the first case of avian influenza in wild birds, which was later detected in backyard and commercial poultry, and in the South-American sea lion (Otaria flavescens) in Tierra del Fuego, in the south of the country. So far, 21 suspected cases have been recorded in humans; however, all of them tested negative for Influenza A virus. Hemagglutinin sequence data of animal viruses analyzed in this report showed that Argentinian viruses clustered together with those isolated in other countries of the region. Epidemiological data suggested the possibility of multiple simultaneous entries of the avian virus, highlighting the role of migratory avian populations in the introduction and dissemination of the disease in Argentina. Continued comprehensive surveillance of these viruses in animals and people worldwide, along with ongoing preparedness efforts, are critical to determine the public health risk.
{"title":"Detection and characterization of highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b virus circulating in Argentina in 2023.","authors":"María Carolina Artuso, Vanina Daniela Marchione, Estefanía Benedetti, Paula Bonastre, Ana María Alvarez, Luana Piccini, Angeles Ponde, Evelyn Barrios Benito, Marcos Fabeiro, Karen Waisman, Luciano Coppola, Tomás Poklepovich, Ariana Chamorro, Martín Avaro, Diego Ariel Riva, Andrea Pontoriero, María Eugenia Ferrer, Andrea Marcos, Lorena Dassa, Daniel Caria, Ximena Melon, Rodrigo Emmanuel Balzano Parodi, Ana María Nicola","doi":"10.1016/j.ram.2024.08.002","DOIUrl":"https://doi.org/10.1016/j.ram.2024.08.002","url":null,"abstract":"<p><p>In 2021, avian influenza A (H5N1) clade 2.3.4.4b virus spread to North America and then to Central and South America in October 2022, extending from Colombia to Chile in three months. During 2023, several countries, mostly in the Americas, reported outbreaks in poultry, wild birds and mammals, as well as the emergence of two cases in humans (one in Ecuador in January and one in Chile in March). As of September 20th, 2023, 17 countries in the Americas Region have recorded cases of A (H5N1) in birds and mammals. On February 14th, 2023, Argentina confirmed the first case of avian influenza in wild birds, which was later detected in backyard and commercial poultry, and in the South-American sea lion (Otaria flavescens) in Tierra del Fuego, in the south of the country. So far, 21 suspected cases have been recorded in humans; however, all of them tested negative for Influenza A virus. Hemagglutinin sequence data of animal viruses analyzed in this report showed that Argentinian viruses clustered together with those isolated in other countries of the region. Epidemiological data suggested the possibility of multiple simultaneous entries of the avian virus, highlighting the role of migratory avian populations in the introduction and dissemination of the disease in Argentina. Continued comprehensive surveillance of these viruses in animals and people worldwide, along with ongoing preparedness efforts, are critical to determine the public health risk.</p>","PeriodicalId":21163,"journal":{"name":"Revista Argentina de microbiologia","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}