Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400010
M. J. Correia, J. R. P. Júnior, J. C. Santos, M. Cavalcanti
A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).
{"title":"Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes","authors":"M. J. Correia, J. R. P. Júnior, J. C. Santos, M. Cavalcanti","doi":"10.1590/S0001-37141998000400010","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400010","url":null,"abstract":"A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"131 1","pages":"286-288"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85604375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400001
J. Trevors
Molecular evolution in bacteria is examined with an emphasis on the self-assembly of cells capable of primitive division and growth during early molecular evolution. Also, the possibility that some type of encapsulation structure preceeded biochemical pathways and the assembly of genetic material is examined. These aspects will be considered from an evolutionary perspective.
{"title":"Molecular evolution in bacteria: cell division","authors":"J. Trevors","doi":"10.1590/S0001-37141998000400001","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400001","url":null,"abstract":"Molecular evolution in bacteria is examined with an emphasis on the self-assembly of cells capable of primitive division and growth during early molecular evolution. Also, the possibility that some type of encapsulation structure preceeded biochemical pathways and the assembly of genetic material is examined. These aspects will be considered from an evolutionary perspective.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"8 1","pages":"237-245"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85635484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400016
C. Dias, Ivonyr Abdel Kader, P. d’Azevedo, S. Superti, D. Alves, G. S. Olm
The performance of agar diffusion tests using disks of cefuroxime (30µg) for predicting ceftriaxone susceptibility in 33 isolates of Streptococcus pneumoniae was studied. All 7 resistant isolates to ceftriaxone (MIC ³1.0 µg/ml) exhibited zones of inhibition <28mm. The procedure can be easily adapted to clinical laboratories.
{"title":"Agar diffusion tests with cefuroxime disks for predicting ceftriaxone susceptibility among isolates of Streptococcus pneumoniae","authors":"C. Dias, Ivonyr Abdel Kader, P. d’Azevedo, S. Superti, D. Alves, G. S. Olm","doi":"10.1590/S0001-37141998000400016","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400016","url":null,"abstract":"The performance of agar diffusion tests using disks of cefuroxime (30µg) for predicting ceftriaxone susceptibility in 33 isolates of Streptococcus pneumoniae was studied. All 7 resistant isolates to ceftriaxone (MIC ³1.0 µg/ml) exhibited zones of inhibition <28mm. The procedure can be easily adapted to clinical laboratories.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"33 1","pages":"314-316"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84599741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400006
E. Tondo, C. W. S. Andretta, C. F. V. Souza, A. L. Monteiro, J. Henriques, M. Ayub
Isolou-se uma bacteria gram positiva, esporulada a partir de efluente de fabrica de polpa de celulose. Esse microrganismo, identificado como Bacillus sp. e nomeado IS13, foi capaz de degradar rapidamente o composto orgânico clorado 4,5,6-tricloroguaiacol (4,5,6-TCG) presente em meio de cultura a uma concentracao de 50mg/L. Essa concentracao equivale a 3x104 vezes mais 4,5,6-TCG que a concentracao encontrada no efluente original. A biodegradacao desse composto foi analisada por espectrofotometria de varredura e cromatografia gasosa. A falta de sub-produtos de degradacao sugeriu a verificacao da possibilidade de adsorcao e absorcao celular do 4,5,6-TCG ao inves de biodegradacao propriamente dita. Nao foram encontrados tracos de 4,5,6-TCG apos lise celular com lisozima e SDS e nao houve desprendimento desse composto apos agitacao vigorosa. Logo, o desaparecimento do 4,5,6-TCG do meio de cultura analisado foi interpretado como biodegradacao devido ao metabolismo do Bacillus sp. IS13. A partir desse microrganismo, buscou-se isolar plasmideos utilizando diferentes protocolos. Os melhores resultados foram obtidos atraves do metodo do CTAB, porem nao encontraram-se plasmideos no isolado IS13. Os resultados sugerem que a alta taxa de degradacao do 4,5,6-TCG e mediada por genes presentes no cromossomo bacteriano. A importância desse trabalho encontra-se na possibilidade de utilizacao do Bacillus sp. IS13 como inoculo em plantas de efluentes industriais, a fim de biodegradar compostos orgânicos clorados presentes nesses locais.
{"title":"High biodegradation levels of 4,5,6-trichloroguaiacol by Bacillus sp. isolated from cellulose pulp mill effluent","authors":"E. Tondo, C. W. S. Andretta, C. F. V. Souza, A. L. Monteiro, J. Henriques, M. Ayub","doi":"10.1590/S0001-37141998000400006","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400006","url":null,"abstract":"Isolou-se uma bacteria gram positiva, esporulada a partir de efluente de fabrica de polpa de celulose. Esse microrganismo, identificado como Bacillus sp. e nomeado IS13, foi capaz de degradar rapidamente o composto orgânico clorado 4,5,6-tricloroguaiacol (4,5,6-TCG) presente em meio de cultura a uma concentracao de 50mg/L. Essa concentracao equivale a 3x104 vezes mais 4,5,6-TCG que a concentracao encontrada no efluente original. A biodegradacao desse composto foi analisada por espectrofotometria de varredura e cromatografia gasosa. A falta de sub-produtos de degradacao sugeriu a verificacao da possibilidade de adsorcao e absorcao celular do 4,5,6-TCG ao inves de biodegradacao propriamente dita. Nao foram encontrados tracos de 4,5,6-TCG apos lise celular com lisozima e SDS e nao houve desprendimento desse composto apos agitacao vigorosa. Logo, o desaparecimento do 4,5,6-TCG do meio de cultura analisado foi interpretado como biodegradacao devido ao metabolismo do Bacillus sp. IS13. A partir desse microrganismo, buscou-se isolar plasmideos utilizando diferentes protocolos. Os melhores resultados foram obtidos atraves do metodo do CTAB, porem nao encontraram-se plasmideos no isolado IS13. Os resultados sugerem que a alta taxa de degradacao do 4,5,6-TCG e mediada por genes presentes no cromossomo bacteriano. A importância desse trabalho encontra-se na possibilidade de utilizacao do Bacillus sp. IS13 como inoculo em plantas de efluentes industriais, a fim de biodegradar compostos orgânicos clorados presentes nesses locais.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"83 1","pages":"265-270"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83810019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400017
Elza F. A. Smânia, Artur Smânia Júnior, C. Loguercio-Leite
Among three strains of Pycnoporus sanguineus, MIP 89007 produced more cinnabarin than MIP 95001 and MIP 95002. The antimicrobial activity of cinnabarin was tested against 11 species of bacteria isolated from food. Bacillus cereus and Leuconostoc plantarum were the most sensitive to cinnabarin, being inhibited by 0.0625 mg/ml. Klebsiella pneumoniae was the least sensitive (>4.0 mg/ml).
{"title":"Cinnabarin synthesis by Pycnoporus sanguineus strains and antimicrobial activity against bacteria from food products","authors":"Elza F. A. Smânia, Artur Smânia Júnior, C. Loguercio-Leite","doi":"10.1590/S0001-37141998000400017","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400017","url":null,"abstract":"Among three strains of Pycnoporus sanguineus, MIP 89007 produced more cinnabarin than MIP 95001 and MIP 95002. The antimicrobial activity of cinnabarin was tested against 11 species of bacteria isolated from food. Bacillus cereus and Leuconostoc plantarum were the most sensitive to cinnabarin, being inhibited by 0.0625 mg/ml. Klebsiella pneumoniae was the least sensitive (>4.0 mg/ml).","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"3 1","pages":"317-320"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87096786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400009
A. Ferreira
Intra and extracellular nuclease production by strains of Aspergillus niger and Aspergillus nidulans was estimated using a modified DNAse test agar and cell-free extract assays. Differences in the production of nucleases by A. niger and A. nidulans were observed. These observations suggest that the DNAse test agar can be helpful for a quick screening for some types of nucleases in filamentous fungi. The assays using cell-free extracts can also be useful for initial characterization of other types of nucleases.
{"title":"Intra and extracellular nuclease production by Aspergillus niger and Aspergillus nidulans","authors":"A. Ferreira","doi":"10.1590/S0001-37141998000400009","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400009","url":null,"abstract":"Intra and extracellular nuclease production by strains of Aspergillus niger and Aspergillus nidulans was estimated using a modified DNAse test agar and cell-free extract assays. Differences in the production of nucleases by A. niger and A. nidulans were observed. These observations suggest that the DNAse test agar can be helpful for a quick screening for some types of nucleases in filamentous fungi. The assays using cell-free extracts can also be useful for initial characterization of other types of nucleases.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"39 1","pages":"282-285"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87327577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400012
P. Pinto, R. Raposeiras, A. Macedo, L. Seldin, E. Paíva, N. Sá
High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45oC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici strains. Certain R. leguminosarum bv. phaseoli strains had significant alterations in plant dry weight and DNA patterns obtained by AP-PCR method. R. tropici strains (with the exception of FJ2.21) were more stable than R. leguminosarum bv. phaseoli strains because no significant phenotypic alterations were observed following thermal treatments and they maintained their original genotypic pattern after inoculation in plants.
{"title":"Effects of high temperature on survival, symbiotic performance and genomic modifications of bean nodulating Rhizobium strains","authors":"P. Pinto, R. Raposeiras, A. Macedo, L. Seldin, E. Paíva, N. Sá","doi":"10.1590/S0001-37141998000400012","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400012","url":null,"abstract":"High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45oC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici strains. Certain R. leguminosarum bv. phaseoli strains had significant alterations in plant dry weight and DNA patterns obtained by AP-PCR method. R. tropici strains (with the exception of FJ2.21) were more stable than R. leguminosarum bv. phaseoli strains because no significant phenotypic alterations were observed following thermal treatments and they maintained their original genotypic pattern after inoculation in plants.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"49 1","pages":"295-300"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82678655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400004
A. Ururahy, M. D. M. Marins, R. L. Vital, I. Gabardo, N. Pereira
Large amounts of oily sludge are generated as residues by the oil industry, representing a real problem for refineries. This work studied the technical viability of treating oily sludge biologically, through stimulation of native microorganisms, at bench scale. Such microorganisms were able to grow in a medium containing oily sludge as the only carbon and energy sources. Two oily sludge concentrations were studied, 5% (v/v) and 10% (v/v), with a C:N ratio of 100:1. Higher microbial populations were observed in the first case. Substrate inhibition and/or toxic effect took place in the second case. The importance of aeration on the microbial activity and on the biodegradation of the residue was ascertained. In terms of n-paraffins, pristane and phytane consumption, maximum global efficiency of 76.9% (w/w) was achieved, in a medium containing 5% (v/v) of oily sludge. Bacteria of the genus Pseudomonas predominated. Two yeast species were also identified and two filamentous fungi were isolated.
{"title":"Effect of aeration on biodegradation of petroleum waste","authors":"A. Ururahy, M. D. M. Marins, R. L. Vital, I. Gabardo, N. Pereira","doi":"10.1590/S0001-37141998000400004","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400004","url":null,"abstract":"Large amounts of oily sludge are generated as residues by the oil industry, representing a real problem for refineries. This work studied the technical viability of treating oily sludge biologically, through stimulation of native microorganisms, at bench scale. Such microorganisms were able to grow in a medium containing oily sludge as the only carbon and energy sources. Two oily sludge concentrations were studied, 5% (v/v) and 10% (v/v), with a C:N ratio of 100:1. Higher microbial populations were observed in the first case. Substrate inhibition and/or toxic effect took place in the second case. The importance of aeration on the microbial activity and on the biodegradation of the residue was ascertained. In terms of n-paraffins, pristane and phytane consumption, maximum global efficiency of 76.9% (w/w) was achieved, in a medium containing 5% (v/v) of oily sludge. Bacteria of the genus Pseudomonas predominated. Two yeast species were also identified and two filamentous fungi were isolated.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"11 1","pages":"254-258"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81749113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400007
Maria R R Silva, C. R. Paula, S. Silva, T. R. Costa, M. R. Costa
Entre oitenta e seis amostras da mucosa oral de pacientes com AIDS, 59 (68,60%) foram positivas para leveduras do genero Candida. A identificacao, feita pela producao de tubo germinativo e clamidosporos e atraves de assimilacao e fermentacao de hidratos de carbono, revelou 52 cepas (88,13%) de C.albicans, 4 (6,77%) de C. tropicalis e 3 (5,08%) de C.krusei. Avaliacao destas leveduras para susceptibilidade in vitro frente a anfotericina B, flucitosina, itraconazol, fluconazol e cetoconazol, foi realizada pelo metodo de diluicao em agar. Comparando-se os valores de concentracao inibitoria minima encontrados com os niveis sericos alcancados por estes antifungicos verificou-se que apenas 8,47% e 5,08% das 59 leveduras foram resistentes a anfotericina B e flucitosina, respectivamente. Foi registrada uma percentagem de cepas resistentes aos derivados azolicos, sendo 25,42% ao itraconazol, 45,76% ao cetoconazol e 66,10% ao fluconazol.
{"title":"Drug resistance of yeasts isolated from oropharyngeal candidiasis in aids patients","authors":"Maria R R Silva, C. R. Paula, S. Silva, T. R. Costa, M. R. Costa","doi":"10.1590/S0001-37141998000400007","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400007","url":null,"abstract":"Entre oitenta e seis amostras da mucosa oral de pacientes com AIDS, 59 (68,60%) foram positivas para leveduras do genero Candida. A identificacao, feita pela producao de tubo germinativo e clamidosporos e atraves de assimilacao e fermentacao de hidratos de carbono, revelou 52 cepas (88,13%) de C.albicans, 4 (6,77%) de C. tropicalis e 3 (5,08%) de C.krusei. Avaliacao destas leveduras para susceptibilidade in vitro frente a anfotericina B, flucitosina, itraconazol, fluconazol e cetoconazol, foi realizada pelo metodo de diluicao em agar. Comparando-se os valores de concentracao inibitoria minima encontrados com os niveis sericos alcancados por estes antifungicos verificou-se que apenas 8,47% e 5,08% das 59 leveduras foram resistentes a anfotericina B e flucitosina, respectivamente. Foi registrada uma percentagem de cepas resistentes aos derivados azolicos, sendo 25,42% ao itraconazol, 45,76% ao cetoconazol e 66,10% ao fluconazol.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"108 1","pages":"271-275"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80736653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-10-01DOI: 10.1590/S0001-37141998000400002
R. C. Minussi, Juliana R. L. Soares-Ramos, J. Coelho, D. O. Silva
The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low concentrations, sugar-cane juice was capable of inducing pectin lyase and polygalacturonase with no cellulase activity in P. griseoroseum.
{"title":"Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum","authors":"R. C. Minussi, Juliana R. L. Soares-Ramos, J. Coelho, D. O. Silva","doi":"10.1590/S0001-37141998000400002","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400002","url":null,"abstract":"The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low concentrations, sugar-cane juice was capable of inducing pectin lyase and polygalacturonase with no cellulase activity in P. griseoroseum.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"15 1","pages":"246-250"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81832173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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