The utilization of pre‐treatment has been a widely adopted method for enhancing the drying characteristics and overall quality of dried products. In this study, ultra‐high‐pressure pre‐treatment technology was applied to examine the impact of varying ultra‐high‐pressure durations (10, 20, 30 min) and ultra‐high‐pressure powers (150, 250, 350 MPa) on the drying attributes, impregnation efficiency, quality, and microstructure of sliced Cistanche during radio frequency vacuum drying. The finding indicated that the ultra‐high‐pressure pre‐treatment was conducive to shortening the drying time; increasing the drying rate of Cistanche ; and effectively retained the content of echinacoside (3.09 ± 0.21 mg/g), Jinshi sericin (0.68 ± 0.05 mg/g), and verbascoside (5.56 ± 0.09 mg/g). The impregnation plots after treatment showed that the cell permeability of the Cistanche pre‐treated with ultra‐high‐pressure was enhanced, and the value of the impregnation rate was increased (1.38–2.04). Compared with the untreated samples, the tissue of the treated Cistanche showed a loose and irregular structure due to the cellular rupture and loss of expansion pressure. These findings not only offer valuable theoretical insights for optimizing processing technology but also hold potential for facilitating the industrialization of sliced Cistanche in future studies.
{"title":"Effect of Ultra‐High‐Pressure Pre‐Treatment on the Physicochemical Properties of Radio Frequency Vacuum Dried Cistanche Slices","authors":"Ao Chen, Fangxin Wan, Guojun Ma, Yanrui Xu, Zepeng Zang, Bowen Wu, Junmin Ma, Xiaopeng Huang","doi":"10.1002/mnfr.70287","DOIUrl":"https://doi.org/10.1002/mnfr.70287","url":null,"abstract":"The utilization of pre‐treatment has been a widely adopted method for enhancing the drying characteristics and overall quality of dried products. In this study, ultra‐high‐pressure pre‐treatment technology was applied to examine the impact of varying ultra‐high‐pressure durations (10, 20, 30 min) and ultra‐high‐pressure powers (150, 250, 350 MPa) on the drying attributes, impregnation efficiency, quality, and microstructure of sliced <jats:italic>Cistanche</jats:italic> during radio frequency vacuum drying. The finding indicated that the ultra‐high‐pressure pre‐treatment was conducive to shortening the drying time; increasing the drying rate of <jats:italic>Cistanche</jats:italic> ; and effectively retained the content of echinacoside (3.09 ± 0.21 mg/g), Jinshi sericin (0.68 ± 0.05 mg/g), and verbascoside (5.56 ± 0.09 mg/g). The impregnation plots after treatment showed that the cell permeability of the <jats:italic>Cistanche</jats:italic> pre‐treated with ultra‐high‐pressure was enhanced, and the value of the impregnation rate was increased (1.38–2.04). Compared with the untreated samples, the tissue of the treated <jats:italic>Cistanche</jats:italic> showed a loose and irregular structure due to the cellular rupture and loss of expansion pressure. These findings not only offer valuable theoretical insights for optimizing processing technology but also hold potential for facilitating the industrialization of sliced <jats:italic>Cistanche</jats:italic> in future studies.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"3 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145611170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wen Xiao, Yan Lu, Liping Wu, Lianwen Zhang, Yufan Hui, Hongmin Luo, Jing Li, Jiangtao Yang
This study aimed to investigate changes in organic acid metabolites in morning urine induced by dietary lemon intake and to explore the relationship between these metabolites and associated metabolic pathways. Fourteen healthy volunteers were prospectively recruited and divided into two sequential phases: a 10‐day baseline phase and a 10‐day lemon intake phase. First‐morning urine samples were collected. Gas chromatography‐mass spectrometry (GC‐MS) was used to analyze organic acid levels. Differential metabolites were identified through multivariate analysis and paired t tests, and functional enrichment analysis was performed to map metabolic pathways. Ten organic acid metabolites exhibited significant changes ( p < 0.05) after lemon intake. Differential metabolites, including citric acid, lactic acid‐2, 2‐hydroxymethyl‐butyric acid‐2, phenylacetic acid‐1, and uracil‐2, were primarily enriched in pathways such as the citrate cycle (TCA cycle), glyoxylate and dicarboxylate metabolism, pyrimidine metabolism, tyrosine metabolism, and fatty acid biosynthesis. Dietary lemon intake increased the concentrations of energy metabolites (citric acid) and saturated fatty acids (palmitic acid‐1), while decreasing the concentrations of organic bases (uracil‐2) and catecholamine metabolites (vanillylmandelic acid‐3 [VMA]). These findings suggest that lemon consumption may influence metabolic pathways linked to renal, oncological, and neurological diseases, providing insights for dietary interventions in disease prevention.
{"title":"The Effect of Dietary Lemon Intake on Organic Acids in Morning Urine","authors":"Wen Xiao, Yan Lu, Liping Wu, Lianwen Zhang, Yufan Hui, Hongmin Luo, Jing Li, Jiangtao Yang","doi":"10.1002/mnfr.70321","DOIUrl":"https://doi.org/10.1002/mnfr.70321","url":null,"abstract":"This study aimed to investigate changes in organic acid metabolites in morning urine induced by dietary lemon intake and to explore the relationship between these metabolites and associated metabolic pathways. Fourteen healthy volunteers were prospectively recruited and divided into two sequential phases: a 10‐day baseline phase and a 10‐day lemon intake phase. First‐morning urine samples were collected. Gas chromatography‐mass spectrometry (GC‐MS) was used to analyze organic acid levels. Differential metabolites were identified through multivariate analysis and paired <jats:italic>t</jats:italic> tests, and functional enrichment analysis was performed to map metabolic pathways. Ten organic acid metabolites exhibited significant changes ( <jats:italic>p</jats:italic> < 0.05) after lemon intake. Differential metabolites, including citric acid, lactic acid‐2, 2‐hydroxymethyl‐butyric acid‐2, phenylacetic acid‐1, and uracil‐2, were primarily enriched in pathways such as the citrate cycle (TCA cycle), glyoxylate and dicarboxylate metabolism, pyrimidine metabolism, tyrosine metabolism, and fatty acid biosynthesis. Dietary lemon intake increased the concentrations of energy metabolites (citric acid) and saturated fatty acids (palmitic acid‐1), while decreasing the concentrations of organic bases (uracil‐2) and catecholamine metabolites (vanillylmandelic acid‐3 [VMA]). These findings suggest that lemon consumption may influence metabolic pathways linked to renal, oncological, and neurological diseases, providing insights for dietary interventions in disease prevention.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"104 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145611056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yunfeng Zhang, Xing Wang, Jun Li, Ruizhen Huang, Penglin Zhang, Zhenfa Tan, Yu Wan, Honglin Hu
Overactive bladder (OAB) is a prevalent urinary dysfunction with a substantial burden. Diet, gut microbiota, and psychological factors such as depression are increasingly implicated in disease pathways, yet evidence from large‐scale studies remains limited. This study investigates the association between the dietary index of gut microbiota (DI‐GM) and OAB using weighted regression and examines whether depressive symptoms serve as a potential mediator through mediation analysis. Data from 30 014 adults in the 2007–2020 National Health and Nutrition Examination Survey (NHANES) were analyzed. DI‐GM scores were derived from dietary recalls; OAB was defined by standardized symptom criteria; depressive symptoms were measured using the Patient Health Questionnaire‐9 (PHQ‐9). Logistic regression, sensitivity, and mediation effect analysis were conducted. Among participants, 6320 had OAB (weighted prevalence 21.0%). Higher DI‐GM scores were associated with lower odds of OAB (fully adjusted odds ratio [OR] = 0.956, p < 0.001), mainly driven by beneficial dietary components. Depressive symptoms mediated 25.8% of the association, rising to 35.4% for beneficial components. A gut microbiota‐supportive dietary pattern is inversely associated with OAB prevalence, underscoring the importance of diet quality in bladder health. Depressive symptoms potentially mediated this link, suggesting dietary interventions may be complemented by psychological support.
{"title":"The Potential Mediating Role of Depressive Symptoms in the Inverse Association Between Dietary Index of Gut Microbiota and Overactive Bladder: A Cross‐Sectional Study","authors":"Yunfeng Zhang, Xing Wang, Jun Li, Ruizhen Huang, Penglin Zhang, Zhenfa Tan, Yu Wan, Honglin Hu","doi":"10.1002/mnfr.70349","DOIUrl":"https://doi.org/10.1002/mnfr.70349","url":null,"abstract":"Overactive bladder (OAB) is a prevalent urinary dysfunction with a substantial burden. Diet, gut microbiota, and psychological factors such as depression are increasingly implicated in disease pathways, yet evidence from large‐scale studies remains limited. This study investigates the association between the dietary index of gut microbiota (DI‐GM) and OAB using weighted regression and examines whether depressive symptoms serve as a potential mediator through mediation analysis. Data from 30 014 adults in the 2007–2020 National Health and Nutrition Examination Survey (NHANES) were analyzed. DI‐GM scores were derived from dietary recalls; OAB was defined by standardized symptom criteria; depressive symptoms were measured using the Patient Health Questionnaire‐9 (PHQ‐9). Logistic regression, sensitivity, and mediation effect analysis were conducted. Among participants, 6320 had OAB (weighted prevalence 21.0%). Higher DI‐GM scores were associated with lower odds of OAB (fully adjusted odds ratio [OR] = 0.956, <jats:italic>p</jats:italic> < 0.001), mainly driven by beneficial dietary components. Depressive symptoms mediated 25.8% of the association, rising to 35.4% for beneficial components. A gut microbiota‐supportive dietary pattern is inversely associated with OAB prevalence, underscoring the importance of diet quality in bladder health. Depressive symptoms potentially mediated this link, suggesting dietary interventions may be complemented by psychological support.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"1 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145611057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deborah Emanuelle de Albuquerque Lemos, Evandro Leite de Souza, Micaelle Oliveira de Luna Freire, Jaielison Yandro Pereira da Silva, Paulo César Trindade da Costa, Josiane de Campos Cruz, Alisson Macário de Oliveira, João Felipe Mota, José Luiz de Brito Alves
This study investigated the effects of a nutraceutical combining jabuticaba peel ( Myrciaria jaboticaba (Vell.) Berg) and Limosilactobacillus fermentum strains on gut microbiota, glycaemic and lipid profiles, and inflammatory markers in rats with gestational diabetes mellitus (GDM), as well as its effects on cardiac autonomic function in the offspring. Pregnant Wistar rats were assigned to control (CTL), GDM, and GDM receiving the nutraceutical (GDM+Nut). The nutraceutical (2 g/kg) or vehicle (PBS) was administered twice daily. After weaning, the offspring were fed laboratory chow until 80 days of age. Nutraceutical administration improved glucose tolerance and reduced serum inflammatory cytokines (TNF‐α and IL‐6). Beta diversity was increased without altering alpha diversity indices in the GDM+Nut. Nutraceuticals increased the abundance of short‐chain fatty acid‐producing bacteria. Maternal supplementation protected against autonomic dysfunction in the offspring. Nutraceutical supplementation shows potential as an alternative therapy for the treatment of GDM, while mitigating autonomic dysfunction in offspring.
{"title":"Synbiotic Nutraceutical Mitigates Gestational Diabetes Effects and Cardiovascular Dysfunction in Rat Offspring","authors":"Deborah Emanuelle de Albuquerque Lemos, Evandro Leite de Souza, Micaelle Oliveira de Luna Freire, Jaielison Yandro Pereira da Silva, Paulo César Trindade da Costa, Josiane de Campos Cruz, Alisson Macário de Oliveira, João Felipe Mota, José Luiz de Brito Alves","doi":"10.1002/mnfr.70340","DOIUrl":"https://doi.org/10.1002/mnfr.70340","url":null,"abstract":"This study investigated the effects of a nutraceutical combining jabuticaba peel ( <jats:italic>Myrciaria jaboticaba</jats:italic> (Vell.) Berg) and <jats:italic>Limosilactobacillus fermentum</jats:italic> strains on gut microbiota, glycaemic and lipid profiles, and inflammatory markers in rats with gestational diabetes mellitus (GDM), as well as its effects on cardiac autonomic function in the offspring. Pregnant Wistar rats were assigned to control (CTL), GDM, and GDM receiving the nutraceutical (GDM+Nut). The nutraceutical (2 g/kg) or vehicle (PBS) was administered twice daily. After weaning, the offspring were fed laboratory chow until 80 days of age. Nutraceutical administration improved glucose tolerance and reduced serum inflammatory cytokines (TNF‐α and IL‐6). Beta diversity was increased without altering alpha diversity indices in the GDM+Nut. Nutraceuticals increased the abundance of short‐chain fatty acid‐producing bacteria. Maternal supplementation protected against autonomic dysfunction in the offspring. Nutraceutical supplementation shows potential as an alternative therapy for the treatment of GDM, while mitigating autonomic dysfunction in offspring.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"8 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145611058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loredana d'Ovidio,Débora Preceliano de Oliveira,Iskra Vitanova Ivanova,Manuela Vaz-Velho,Bernadette Dora Gombossy de Melo Franco,Svetoslav Dimitrov Todorov
Listeria monocytogenes is a highly virulent foodborne pathogen responsible for listeriosis, a severe infection threatening vulnerable populations such as pregnant women, newborns, the elderly, and immunocompromised individuals. Its resilience, surviving low pH, reduced water activity, and refrigeration, makes it a formidable contaminant in food systems. Traditional control methods include heat treatment, high-pressure processing, irradiation, and acidification, all aimed at reducing bacterial load in ready-to-eat foods. WHO and FAO guidelines emphasize minimizing contamination and growth. Emerging strategies target gene expression to curb virulence and survival. External signals like chitin can suppress pathogenic genes, while nucleomodulins alter host chromatin to disrupt infection. Regulatory proteins such as MogR and GmaR modulate motility-related genes, and selective pressures from antimicrobials or bacteriophages can reshape bacterial behavior. Genetic tools like CRISPR-Cas9 offer precision editing of key genes. Additional interventions include environmental adjustments (temperature, pH, salinity), bacteriophage applications (e.g., PhageGuard Listex, ListShield), and competitive exclusion via beneficial microbes. Natural antimicrobials like bacteriocins (nisin, pediocin, enterocin, plantaricins, and lactocin S) disrupt cell walls and membranes. Phenolic compounds such as allicin, eugenol, and curcumin also exhibit inhibitory effects. Combining these approaches is vital for effective control and enhanced food safety.
{"title":"Listeria monocytogenes-Can We Reduce or Eliminate It From Food Commodities?","authors":"Loredana d'Ovidio,Débora Preceliano de Oliveira,Iskra Vitanova Ivanova,Manuela Vaz-Velho,Bernadette Dora Gombossy de Melo Franco,Svetoslav Dimitrov Todorov","doi":"10.1002/mnfr.70329","DOIUrl":"https://doi.org/10.1002/mnfr.70329","url":null,"abstract":"Listeria monocytogenes is a highly virulent foodborne pathogen responsible for listeriosis, a severe infection threatening vulnerable populations such as pregnant women, newborns, the elderly, and immunocompromised individuals. Its resilience, surviving low pH, reduced water activity, and refrigeration, makes it a formidable contaminant in food systems. Traditional control methods include heat treatment, high-pressure processing, irradiation, and acidification, all aimed at reducing bacterial load in ready-to-eat foods. WHO and FAO guidelines emphasize minimizing contamination and growth. Emerging strategies target gene expression to curb virulence and survival. External signals like chitin can suppress pathogenic genes, while nucleomodulins alter host chromatin to disrupt infection. Regulatory proteins such as MogR and GmaR modulate motility-related genes, and selective pressures from antimicrobials or bacteriophages can reshape bacterial behavior. Genetic tools like CRISPR-Cas9 offer precision editing of key genes. Additional interventions include environmental adjustments (temperature, pH, salinity), bacteriophage applications (e.g., PhageGuard Listex, ListShield), and competitive exclusion via beneficial microbes. Natural antimicrobials like bacteriocins (nisin, pediocin, enterocin, plantaricins, and lactocin S) disrupt cell walls and membranes. Phenolic compounds such as allicin, eugenol, and curcumin also exhibit inhibitory effects. Combining these approaches is vital for effective control and enhanced food safety.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"37 1","pages":"e70329"},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aysu Shahin,Alastair B Ross,Robin M Turner,Jim I Mann,Andrew N Reynolds
Specific metabolites detected in human biofluid after the ingestion of food have been proposed as objective markers of dietary intake, yet their evaluation across diverse dietary settings remains limited. This study sought to build a panel of biomarkers of food intake (BFI) representing major food groups and compared their plasma concentrations with self-reported dietary intakes during a randomized three-arm trial. Interventions were of delivered groceries either high in fiber or unsaturated fats, or no groceries for 12 weeks. Plasma samples from 245 adults (mean age: 63.4 ± 9.5 years) were analyzed pre- and post-dietary intervention. Gas chromatography-mass spectrometry detected 104 known food-related metabolites. Pre-intervention, modest Spearman's rank correlation coefficients were observed for red meat (1-methylhistidine ρ = 0.13; urea ρ = 0.19), fish (anserine ρ = 0.24; 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid and docosahexaenoic acid ρ = 0.13), fruit (ascorbic acid, hippuric acid, 4-coumaric acid, linoleic acid ρ = 0.14-0.17), and legumes (pipecolic acid ρ = 0.16-0.19) using 4-day food dairy data. Post-intervention, additional modest correlations were observed between biomarkers and self-reported dietary intakes. We identified over 40 plasma metabolites associated with specific foods or food groups, supporting their use in a multimarker panel for objective dietary assessment. No biomarkers were identified for vegetables, dairy, and chocolate, highlighting key gaps for future research to understand and validate BFI for their broader application.
{"title":"Assessing a Panel of Biomarkers in Plasma Reflecting Dietary Intakes: Data From a Large Randomized Controlled Trial.","authors":"Aysu Shahin,Alastair B Ross,Robin M Turner,Jim I Mann,Andrew N Reynolds","doi":"10.1002/mnfr.70334","DOIUrl":"https://doi.org/10.1002/mnfr.70334","url":null,"abstract":"Specific metabolites detected in human biofluid after the ingestion of food have been proposed as objective markers of dietary intake, yet their evaluation across diverse dietary settings remains limited. This study sought to build a panel of biomarkers of food intake (BFI) representing major food groups and compared their plasma concentrations with self-reported dietary intakes during a randomized three-arm trial. Interventions were of delivered groceries either high in fiber or unsaturated fats, or no groceries for 12 weeks. Plasma samples from 245 adults (mean age: 63.4 ± 9.5 years) were analyzed pre- and post-dietary intervention. Gas chromatography-mass spectrometry detected 104 known food-related metabolites. Pre-intervention, modest Spearman's rank correlation coefficients were observed for red meat (1-methylhistidine ρ = 0.13; urea ρ = 0.19), fish (anserine ρ = 0.24; 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid and docosahexaenoic acid ρ = 0.13), fruit (ascorbic acid, hippuric acid, 4-coumaric acid, linoleic acid ρ = 0.14-0.17), and legumes (pipecolic acid ρ = 0.16-0.19) using 4-day food dairy data. Post-intervention, additional modest correlations were observed between biomarkers and self-reported dietary intakes. We identified over 40 plasma metabolites associated with specific foods or food groups, supporting their use in a multimarker panel for objective dietary assessment. No biomarkers were identified for vegetables, dairy, and chocolate, highlighting key gaps for future research to understand and validate BFI for their broader application.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"243 1","pages":"e70334"},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Moringa oleifera (MO), also known as drumstick, has gained importance as a medicinal plant. In this experimental study, the prebiotic activity and glucose-balancing potential of Moringa oleifera have been investigated. Twenty-four male albino rats weighing 200-250 g were kept in the animal house. Streptozotocin-induced diabetic rats were randomly divided into four groups (n = 6 in each group). Control group (G0) rats were given Metformin 100 mg/kg/day, treatment groups G1, G2, and G3 rats were provided with MO leaves extract 100, 200, and 300 mg/kg/day, respectively, with a standard basal diet, and were monitored twice a week for 21 days. The results of stool analysis showed the growth of Lactobacillus, which is evident by increased count of 104 CFU Lactobacillus in groups G2 and G3 compared to the control group 103 CFU Lactobacillus, while higher 105 CFU Lactobacillus were observed in group G1 treated with 100 mg/kg/day of MO leaves extract. Anti-hyperglycemic results are more evident on a dose of 100 mg/kg/day from 152.50 ± 7.7 to 119 ± 7.07 (p value = 0.010) compared to other doses (p > 0.05). This study's results conclude that Moringa oleifera has promising prebiotic and anti-hyperglycemic properties and need further experimentation for a longer duration to establish the effective dose of Moringa oleifera.
{"title":"Gut Microbial Modulation as Precursor for Blood Glucose Regulation Through Moringa Oleifera Leaves Aqueous Extract-An Experimental Study.","authors":"Aamina Sabir,Shaista Jabeen,Hafsa Kamran,Sana Farooq,Fareeha Bashir","doi":"10.1002/mnfr.70335","DOIUrl":"https://doi.org/10.1002/mnfr.70335","url":null,"abstract":"Moringa oleifera (MO), also known as drumstick, has gained importance as a medicinal plant. In this experimental study, the prebiotic activity and glucose-balancing potential of Moringa oleifera have been investigated. Twenty-four male albino rats weighing 200-250 g were kept in the animal house. Streptozotocin-induced diabetic rats were randomly divided into four groups (n = 6 in each group). Control group (G0) rats were given Metformin 100 mg/kg/day, treatment groups G1, G2, and G3 rats were provided with MO leaves extract 100, 200, and 300 mg/kg/day, respectively, with a standard basal diet, and were monitored twice a week for 21 days. The results of stool analysis showed the growth of Lactobacillus, which is evident by increased count of 104 CFU Lactobacillus in groups G2 and G3 compared to the control group 103 CFU Lactobacillus, while higher 105 CFU Lactobacillus were observed in group G1 treated with 100 mg/kg/day of MO leaves extract. Anti-hyperglycemic results are more evident on a dose of 100 mg/kg/day from 152.50 ± 7.7 to 119 ± 7.07 (p value = 0.010) compared to other doses (p > 0.05). This study's results conclude that Moringa oleifera has promising prebiotic and anti-hyperglycemic properties and need further experimentation for a longer duration to establish the effective dose of Moringa oleifera.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"70 1","pages":"e70335"},"PeriodicalIF":5.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dietary functional oligosaccharides exhibit anti‐inflammatory activity and regulatory impact on glucose metabolism. However, the protective effect of neoagarotetraose (NAT) against diabetes progression and the underlying molecular mechanisms remain unclear. This study investigated the hypoglycemic effect of NAT in diabetic mice. NAT was supplemented for 12 weeks in mice fed with a high‐fat diet (HFD). In HFD‐fed mice, NAT intervention (600 mg/kg) reduced the fasting glucose and insulin concentrations by 35.9% and 30.1% ( p < 0.01), and improved systemic inflammation and dyslipidemia. Moreover, diabetic mice induced by a high‐fat and high‐sugar diet plus streptozotocin were administered NAT for 7 weeks. NAT treatment lowered the area under the oral glucose and insulin tolerance test curves by 31.0% and 48.9% ( p < 0.01). NAT decreased the phosphorylation of nuclear factor kappa‐B to alleviate intestinal inflammation caused by diabetes mellitus. NAT reversed the derangements of hepatic glucose metabolism as evidenced by the activated AMPK/PGC‐1α signaling pathway and decreased mRNA expression of inflammatory factors. Furthermore, the imbalanced gut microbiota of diabetic mice was reshaped by NAT treatment. NAT attenuates hyperglycemia through inflammation amelioration and glucose metabolism improvement, thus mitigating the development of diabetes mellitus in mice.
{"title":"Neoagarotetraose Attenuates Hyperglycemia Through Inflammation Amelioration and Glucose Metabolism Improvement","authors":"Jiaobo Cheng, Qiaojuan Yan, Dan Liu, Chenxuan Wu, Yanxiao Li, Zhengqiang Jiang","doi":"10.1002/mnfr.70323","DOIUrl":"https://doi.org/10.1002/mnfr.70323","url":null,"abstract":"Dietary functional oligosaccharides exhibit anti‐inflammatory activity and regulatory impact on glucose metabolism. However, the protective effect of neoagarotetraose (NAT) against diabetes progression and the underlying molecular mechanisms remain unclear. This study investigated the hypoglycemic effect of NAT in diabetic mice. NAT was supplemented for 12 weeks in mice fed with a high‐fat diet (HFD). In HFD‐fed mice, NAT intervention (600 mg/kg) reduced the fasting glucose and insulin concentrations by 35.9% and 30.1% ( <jats:italic>p </jats:italic> < 0.01), and improved systemic inflammation and dyslipidemia. Moreover, diabetic mice induced by a high‐fat and high‐sugar diet plus streptozotocin were administered NAT for 7 weeks. NAT treatment lowered the area under the oral glucose and insulin tolerance test curves by 31.0% and 48.9% ( <jats:italic>p</jats:italic> < 0.01). NAT decreased the phosphorylation of nuclear factor kappa‐B to alleviate intestinal inflammation caused by diabetes mellitus. NAT reversed the derangements of hepatic glucose metabolism as evidenced by the activated AMPK/PGC‐1α signaling pathway and decreased mRNA expression of inflammatory factors. Furthermore, the imbalanced gut microbiota of diabetic mice was reshaped by NAT treatment. NAT attenuates hyperglycemia through inflammation amelioration and glucose metabolism improvement, thus mitigating the development of diabetes mellitus in mice.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"137 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145583340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ioanna Ploumaki, Samya El Sayed, Awurakua Asamoah-Mensah, Valeria I. Macri, James H. Segars, Md Soriful Islam
The cover image is based on the article The Potential of Vitamin D and Epigallocatechin Gallate (EGCG) for the Treatment of Uterine Fibroids: Evidence From In Vitro to Clinical Studies by Md Soriful Islam et al., https://doi.org/10.1002/mnfr.70231
{"title":"Front Cover: The Potential of Vitamin D and Epigallocatechin Gallate (EGCG) for the Treatment of Uterine Fibroids: Evidence From In Vitro to Clinical Studies","authors":"Ioanna Ploumaki, Samya El Sayed, Awurakua Asamoah-Mensah, Valeria I. Macri, James H. Segars, Md Soriful Islam","doi":"10.1002/mnfr.70314","DOIUrl":"10.1002/mnfr.70314","url":null,"abstract":"<p>The cover image is based on the article The Potential of Vitamin D and Epigallocatechin Gallate (EGCG) for the Treatment of Uterine Fibroids: Evidence From In Vitro to Clinical Studies by Md Soriful Islam et al., https://doi.org/10.1002/mnfr.70231\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"69 22","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mnfr.70314","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145583593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：