Glucosinolates (GSLs), the characteristic sulfur-rich components of Brassicaceae plants and their therapeutically active hydrolysis products, including aliphatic isothiocyanates (sulforaphane, allyl isothiocyanate); aromatic isothiocyanate (benzyl isothiocyanate); and indole-3-carbinol metabolites, predominantly contribute to their anti-diabetic efficacy. They possess the propensity to reduce insulin resistance, enhance antioxidant defense mechanisms via Nrf2 activation, impede inflammation via NF-κB inhibition, prevent exaggerated hepatic glucose production, restore β-cell function via activation of Nrf2 and inhibition of NF-κB, regulate lipid and glucose metabolism via PGC-1α and Nrf2-dependent mitochondrial regulation, regulation of AMPK, downregulation of SREBP1, and activate transient receptor potential (TRP vanilloid type 1 and TRP ankyrin 1). The current review summarizes the underlying mechanistic pathways and research outcomes that follow the anti-diabetic intervention of GSLs and their derivatives. Although consumption of GSLs has been linked to the prevention of diabetic conditions, most of the studies investigating underlying mechanisms and other evaluations are based on in vitro and in vivo studies. Moreover, the currently available clinical studies have certain limitations due to heterogeneity in the source, dosage, formulation, study designs, lack of proper standardization, inadequate sample sizes, and clearly defined target populations. Thus, there is a pressing need for more clinical studies using standardized glucosinolate supplements.
{"title":"Glucosinolates Hydrolysis Products: Promising Bioactives for the Prevention of Diabetes.","authors":"Rohini Bhat,Sugandh Saxena,Manu Khajuria,Dhiraj Vyas","doi":"10.1002/mnfr.70302","DOIUrl":"https://doi.org/10.1002/mnfr.70302","url":null,"abstract":"Glucosinolates (GSLs), the characteristic sulfur-rich components of Brassicaceae plants and their therapeutically active hydrolysis products, including aliphatic isothiocyanates (sulforaphane, allyl isothiocyanate); aromatic isothiocyanate (benzyl isothiocyanate); and indole-3-carbinol metabolites, predominantly contribute to their anti-diabetic efficacy. They possess the propensity to reduce insulin resistance, enhance antioxidant defense mechanisms via Nrf2 activation, impede inflammation via NF-κB inhibition, prevent exaggerated hepatic glucose production, restore β-cell function via activation of Nrf2 and inhibition of NF-κB, regulate lipid and glucose metabolism via PGC-1α and Nrf2-dependent mitochondrial regulation, regulation of AMPK, downregulation of SREBP1, and activate transient receptor potential (TRP vanilloid type 1 and TRP ankyrin 1). The current review summarizes the underlying mechanistic pathways and research outcomes that follow the anti-diabetic intervention of GSLs and their derivatives. Although consumption of GSLs has been linked to the prevention of diabetic conditions, most of the studies investigating underlying mechanisms and other evaluations are based on in vitro and in vivo studies. Moreover, the currently available clinical studies have certain limitations due to heterogeneity in the source, dosage, formulation, study designs, lack of proper standardization, inadequate sample sizes, and clearly defined target populations. Thus, there is a pressing need for more clinical studies using standardized glucosinolate supplements.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"85 1","pages":"e70302"},"PeriodicalIF":5.2,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145440617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sebastian Stricker, Jan De Laffolie, Klaus-Peter Zimmer, Silvia Rudloff
The cover image is based on the article Microbial transglutaminase increases uptake and translocation of gliadin peptides in the human intestinal epithelium by Sebastian Stricker et al., https://doi.org/10.1002/mnfr.70197
{"title":"Front Cover: Microbial Transglutaminase Increases Uptake and Translocation of Gliadin Peptides in the Human Intestinal Epithelium","authors":"Sebastian Stricker, Jan De Laffolie, Klaus-Peter Zimmer, Silvia Rudloff","doi":"10.1002/mnfr.70300","DOIUrl":"https://doi.org/10.1002/mnfr.70300","url":null,"abstract":"<p>The cover image is based on the article Microbial transglutaminase increases uptake and translocation of gliadin peptides in the human intestinal epithelium by Sebastian Stricker et al., https://doi.org/10.1002/mnfr.70197\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"69 21","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mnfr.70300","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145429292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Celin Mundt, Büşra Yusufoğlu, Daniel Kudenko, Kerem Mertoğlu, Tuba Esatbeyoglu
Personalized nutrition (PN) aims to prevent and manage chronic diseases by providing individualized dietary guidance based on genetic, metabolic, and lifestyle data. Artificial intelligence (AI) has become a key enabler in PN by analyzing large‐scale, multiomics datasets in obesity, diabetes, cardiovascular, and gastrointestinal disorders, where digital twins and health knowledge graphs support personalized interventions. Current findings demonstrate that AI models can guide microbiome‐based dietary interventions, and support obesity management, thereby extending the scope of conventional nutritional strategies as supported by deepened bibliometric analyses. This study highlights the global increase in AI‐based PN studies, accelerated by digital health demands and the COVID‐19 pandemic, and the expansion of traditional nutrition strategies through machine learning approaches with the integration of microbiome‐based models and omics. However, challenges such as algorithmic bias, limited generalizability, and data privacy remain. To overcome these issues, diverse datasets, explainable AI approaches, and standardized multicenter validation protocols are proposed. These steps are critical for transforming AI‐supported PN from a conceptual potential into a fair, reliable, and clinically applicable structure. The growing consensus in the literature highlights that AI can support individual and societal health goals by transforming nutrition science through predictive, adaptive, and ethically based approaches.
{"title":"AI‐Driven Personalized Nutrition: Integrating Omics, Ethics, and Digital Health","authors":"Celin Mundt, Büşra Yusufoğlu, Daniel Kudenko, Kerem Mertoğlu, Tuba Esatbeyoglu","doi":"10.1002/mnfr.70293","DOIUrl":"https://doi.org/10.1002/mnfr.70293","url":null,"abstract":"Personalized nutrition (PN) aims to prevent and manage chronic diseases by providing individualized dietary guidance based on genetic, metabolic, and lifestyle data. Artificial intelligence (AI) has become a key enabler in PN by analyzing large‐scale, multiomics datasets in obesity, diabetes, cardiovascular, and gastrointestinal disorders, where digital twins and health knowledge graphs support personalized interventions. Current findings demonstrate that AI models can guide microbiome‐based dietary interventions, and support obesity management, thereby extending the scope of conventional nutritional strategies as supported by deepened bibliometric analyses. This study highlights the global increase in AI‐based PN studies, accelerated by digital health demands and the COVID‐19 pandemic, and the expansion of traditional nutrition strategies through machine learning approaches with the integration of microbiome‐based models and omics. However, challenges such as algorithmic bias, limited generalizability, and data privacy remain. To overcome these issues, diverse datasets, explainable AI approaches, and standardized multicenter validation protocols are proposed. These steps are critical for transforming AI‐supported PN from a conceptual potential into a fair, reliable, and clinically applicable structure. The growing consensus in the literature highlights that AI can support individual and societal health goals by transforming nutrition science through predictive, adaptive, and ethically based approaches.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"44 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Molly Jenee Buccola,Adhini Kuppuswamy Satheesh Babu,Henry A Paz,Nizhoni Dawn Porter,Harini Srinivasan,Rachel Lyn Ricks,Keaton Rosquist,Jose Luis Torres,Ying Zhong,Thunder Jalili,Umesh D Wankhade,Pon Velayutham Anandh Babu
Proton pump inhibitors (PPIs) are commonly used to treat heartburn and acid-related disorders. However, their misuse and prolonged use contribute to gut dysbiosis. This study investigated whether well-known prebiotic dietary sources, blueberries or strawberries, can reverse PPI (omeprazole) induced dysbiosis and gut inflammation by modulating gut microbes. Male C57BL/6J mice (7 weeks old) were fed a diet with or without omeprazole (40 mg/kg diet), blueberry (3.7% in the diet; ∼1.5 human servings) or strawberry (2.35% in the diet; ∼2 human servings) for 12 weeks. Metabolic parameters, gut microbes (in the cecum and colon), and inflammatory markers were assessed. In this study, no changes were observed in metabolic parameters in mice fed a diet supplemented with omeprazole or berries. Second, blueberry or strawberry supplementation at nutritional dosages improved alterations in gut microbial ecology induced by omeprazole, with effects varying between the cecum and colon. Third, strawberry supplementation reduced omeprazole-induced gut inflammation. Fourth, selected genera were either positively or negatively associated with markers of gut inflammation, suggesting that dietary berries can ameliorate inflammatory signaling through modifications in the gut microbiome. Dietary berries represent a potential nutritional strategy for improving PPI-induced gut dysbiosis and inflammation.
{"title":"Dietary Prebiotics Modulate Omeprazole-Induced Alterations in the Gut Microbial Signature.","authors":"Molly Jenee Buccola,Adhini Kuppuswamy Satheesh Babu,Henry A Paz,Nizhoni Dawn Porter,Harini Srinivasan,Rachel Lyn Ricks,Keaton Rosquist,Jose Luis Torres,Ying Zhong,Thunder Jalili,Umesh D Wankhade,Pon Velayutham Anandh Babu","doi":"10.1002/mnfr.70307","DOIUrl":"https://doi.org/10.1002/mnfr.70307","url":null,"abstract":"Proton pump inhibitors (PPIs) are commonly used to treat heartburn and acid-related disorders. However, their misuse and prolonged use contribute to gut dysbiosis. This study investigated whether well-known prebiotic dietary sources, blueberries or strawberries, can reverse PPI (omeprazole) induced dysbiosis and gut inflammation by modulating gut microbes. Male C57BL/6J mice (7 weeks old) were fed a diet with or without omeprazole (40 mg/kg diet), blueberry (3.7% in the diet; ∼1.5 human servings) or strawberry (2.35% in the diet; ∼2 human servings) for 12 weeks. Metabolic parameters, gut microbes (in the cecum and colon), and inflammatory markers were assessed. In this study, no changes were observed in metabolic parameters in mice fed a diet supplemented with omeprazole or berries. Second, blueberry or strawberry supplementation at nutritional dosages improved alterations in gut microbial ecology induced by omeprazole, with effects varying between the cecum and colon. Third, strawberry supplementation reduced omeprazole-induced gut inflammation. Fourth, selected genera were either positively or negatively associated with markers of gut inflammation, suggesting that dietary berries can ameliorate inflammatory signaling through modifications in the gut microbiome. Dietary berries represent a potential nutritional strategy for improving PPI-induced gut dysbiosis and inflammation.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"12 1","pages":"e70307"},"PeriodicalIF":5.2,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Layanne Nascimento Fraga,Dragan Milenkovic,Isabella de Araújo Esteves Duarte,Saivageethi Nuthikattu,Camille Perella Coutinho,Franco Maria Lajolo,Neuza Mariko Aymoto Hassimotto
Consumption of orange juice (OJ) has been linked to a lower incidence of cardiovascular diseases. However, molecular mechanisms underlying these health benefits remain unclear. This study aimed to investigate the molecular pathways involved in the health benefits of chronic OJ consumption through global transcriptomic approach in peripheral blood mononuclear cells (PBMCs). Healthy volunteers consumed 500 mL of OJ daily for 60 days. PBMCs were isolated from the blood, and the gene expression profiling was performed using Clariom microarrays. Genomic analysis revealed 1705 differentially expressed genes, including IL6, IL1β involved in inflammation, GSK3B, RIPK1 related to lipid metabolism, and NAMPT, NLRP3 involved in blood pressure regulation. Moreover, the expression of 66 miRNAs, 19 long-non-coding RNAs (lncRNAs), and 67 small nucleolar RNAs (snoRNAs) was modulated. Moreover, subgroup analysis revealed different transcriptomic responses between normal-weight (NW) and overweight (OW) individuals. Genes related to lipid metabolism and adipogenesis were differentially expressed only in OW, including GSK3B, GRK6, miR-548i, and miR-1292-3p. In contrast, genes related to the inflammation process, like STAT3, MAPK, and miRNAs (miR-1185-2-5p), were observed only in NW. These findings suggest that body weight may influence molecular response to bioactive compounds in OJ and provide information for personalized recommendations on the consumption of flavonoid-rich foods.
{"title":"A Global Transcriptomic Analysis Reveals Body Weight-Specific Molecular Responses to Chronic Orange Juice Consumption in Healthy Individuals.","authors":"Layanne Nascimento Fraga,Dragan Milenkovic,Isabella de Araújo Esteves Duarte,Saivageethi Nuthikattu,Camille Perella Coutinho,Franco Maria Lajolo,Neuza Mariko Aymoto Hassimotto","doi":"10.1002/mnfr.70299","DOIUrl":"https://doi.org/10.1002/mnfr.70299","url":null,"abstract":"Consumption of orange juice (OJ) has been linked to a lower incidence of cardiovascular diseases. However, molecular mechanisms underlying these health benefits remain unclear. This study aimed to investigate the molecular pathways involved in the health benefits of chronic OJ consumption through global transcriptomic approach in peripheral blood mononuclear cells (PBMCs). Healthy volunteers consumed 500 mL of OJ daily for 60 days. PBMCs were isolated from the blood, and the gene expression profiling was performed using Clariom microarrays. Genomic analysis revealed 1705 differentially expressed genes, including IL6, IL1β involved in inflammation, GSK3B, RIPK1 related to lipid metabolism, and NAMPT, NLRP3 involved in blood pressure regulation. Moreover, the expression of 66 miRNAs, 19 long-non-coding RNAs (lncRNAs), and 67 small nucleolar RNAs (snoRNAs) was modulated. Moreover, subgroup analysis revealed different transcriptomic responses between normal-weight (NW) and overweight (OW) individuals. Genes related to lipid metabolism and adipogenesis were differentially expressed only in OW, including GSK3B, GRK6, miR-548i, and miR-1292-3p. In contrast, genes related to the inflammation process, like STAT3, MAPK, and miRNAs (miR-1185-2-5p), were observed only in NW. These findings suggest that body weight may influence molecular response to bioactive compounds in OJ and provide information for personalized recommendations on the consumption of flavonoid-rich foods.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"19 1","pages":"e70299"},"PeriodicalIF":5.2,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Whether conjugated linoleic acid (CLA) improves body composition by altering de novo lipogenesis (DNL) and related pathways in human remains unclear. The study aimed to investigate CLA-induced changes in DNL-derived fatty acids, and related metabolic pathways via plasma metabolome. Erythrocyte fatty acids of 65 participants with elevated body fat percentage before and after a 12-week CLA intervention were analyzed by gas chromatography. Compared with placebo, CLA supplementation decreased erythrocyte C16:1n7 and C18:1n9 while increased C18:3n3 levels (all p < 0.05). The C16:1n7 change was positively correlated with the triglycerides (TG) change, while the C18:3n3 change was negatively correlated with the low-density lipoprotein cholesterol (LDL-C) change. The optimal metabolite panels and metabolite scores predictive of fatty acid changes were identified and generated. TG and visceral adiposity index changes were positively correlated with the metabolite scores of the C16:1n7 change and C18:1n9 change, while metabolite score of the C18:3n3 change was negatively correlated with LDL-C change. Furthermore, DNL fatty acid changes were related to KEGG pathways, including the citrate cycle and butanoate metabolism. In adults with high body fat, 12-week CLA supplementation suppressed DNL activity, associated with changes in plasma and liver lipids, involving energy metabolism pathways. Trial Registration: ClinicalTrials.gov identifier: NCT03915808.
{"title":"Conjugated Linoleic Acid Supplementation Suppresses the De Novo Lipogenesis in Adults With High Body Fat: A Double-Blind, Randomized, Placebo-Controlled Trial.","authors":"Kaizhen Jia,Yongbo She,Yafang He,Juan He,Yifei He,Kun Xu,Guoqing Ma,Wei Li,Chen He,Xinran Feng,Menghan Wang,Amei Tang,Xiaomin Sun,Liyun Kong,Xin Liu","doi":"10.1002/mnfr.70306","DOIUrl":"https://doi.org/10.1002/mnfr.70306","url":null,"abstract":"Whether conjugated linoleic acid (CLA) improves body composition by altering de novo lipogenesis (DNL) and related pathways in human remains unclear. The study aimed to investigate CLA-induced changes in DNL-derived fatty acids, and related metabolic pathways via plasma metabolome. Erythrocyte fatty acids of 65 participants with elevated body fat percentage before and after a 12-week CLA intervention were analyzed by gas chromatography. Compared with placebo, CLA supplementation decreased erythrocyte C16:1n7 and C18:1n9 while increased C18:3n3 levels (all p < 0.05). The C16:1n7 change was positively correlated with the triglycerides (TG) change, while the C18:3n3 change was negatively correlated with the low-density lipoprotein cholesterol (LDL-C) change. The optimal metabolite panels and metabolite scores predictive of fatty acid changes were identified and generated. TG and visceral adiposity index changes were positively correlated with the metabolite scores of the C16:1n7 change and C18:1n9 change, while metabolite score of the C18:3n3 change was negatively correlated with LDL-C change. Furthermore, DNL fatty acid changes were related to KEGG pathways, including the citrate cycle and butanoate metabolism. In adults with high body fat, 12-week CLA supplementation suppressed DNL activity, associated with changes in plasma and liver lipids, involving energy metabolism pathways. Trial Registration: ClinicalTrials.gov identifier: NCT03915808.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"48 1","pages":"e70306"},"PeriodicalIF":5.2,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhuoshuai Liang,Xiaoyue Sun,Huizhen Jin,Wenhui Gao,Xinmeng Hu,Yi Cheng,Jikang Shi,Yawen Liu
Steatotic liver disease is a major global health burden driven by diet, lifestyle, and alcohol use. Coffee and tea-across their diverse subtypes-contain numerous bioactive compounds with antioxidant, anti-inflammatory, and metabolic properties, yet their differential effects on liver health remain unclear. We analyzed data from 26 729 UK Biobank participants who underwent liver MRI between 2016 and 2020. Hepatic fibroinflammation (iron-corrected T1 [cT1]), liver iron, and hepatic fat (proton density fat fraction [PDFF]) were measured by MRI. Coffee and tea intake were self-reported. Generalized linear models assessed their associations with hepatic imaging markers. Compared with non-tea drinkers, those consuming 0.5-1, 2-3, 4-5, or ≥ 6 cups of tea per day had lower odds of elevated cT1 (≥800 ms), liver iron (≥1.8 mg/g), and hepatic fat (PDFF ≥ 5%) (OR range: 0.57-0.94). Coffee consumption was inversely related to liver iron and PDFF (OR range: 0.47-0.94). All tea types were associated with lower fibroinflammation, whereas instant, ground, and decaffeinated coffee were associated with lower PDFF and iron levels. Tea, regardless of type, is associated with lower hepatic fat, iron deposition, and fibroinflammation, while coffee shows similar associations for hepatic fat and iron.
{"title":"Coffee and Tea Intake Is Inversely Associated With Hepatic Fat Deposition, Iron Deposition, and Fibroinflammation in the General Population.","authors":"Zhuoshuai Liang,Xiaoyue Sun,Huizhen Jin,Wenhui Gao,Xinmeng Hu,Yi Cheng,Jikang Shi,Yawen Liu","doi":"10.1002/mnfr.70310","DOIUrl":"https://doi.org/10.1002/mnfr.70310","url":null,"abstract":"Steatotic liver disease is a major global health burden driven by diet, lifestyle, and alcohol use. Coffee and tea-across their diverse subtypes-contain numerous bioactive compounds with antioxidant, anti-inflammatory, and metabolic properties, yet their differential effects on liver health remain unclear. We analyzed data from 26 729 UK Biobank participants who underwent liver MRI between 2016 and 2020. Hepatic fibroinflammation (iron-corrected T1 [cT1]), liver iron, and hepatic fat (proton density fat fraction [PDFF]) were measured by MRI. Coffee and tea intake were self-reported. Generalized linear models assessed their associations with hepatic imaging markers. Compared with non-tea drinkers, those consuming 0.5-1, 2-3, 4-5, or ≥ 6 cups of tea per day had lower odds of elevated cT1 (≥800 ms), liver iron (≥1.8 mg/g), and hepatic fat (PDFF ≥ 5%) (OR range: 0.57-0.94). Coffee consumption was inversely related to liver iron and PDFF (OR range: 0.47-0.94). All tea types were associated with lower fibroinflammation, whereas instant, ground, and decaffeinated coffee were associated with lower PDFF and iron levels. Tea, regardless of type, is associated with lower hepatic fat, iron deposition, and fibroinflammation, while coffee shows similar associations for hepatic fat and iron.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"25 1","pages":"e70310"},"PeriodicalIF":5.2,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sidra Tul‐Muntaha, Allah Rakha, Imran Pasha, Bilal Aslam
This research aims to elucidate the therapeutic potential of polyphenol–protein particle‐enriched beverages in the rat model of acetic acid‐induced colitis. The 50 male Wistar rats were divided into five groups: G 0 as the negative control, G 1 as the positive control, G 2 as the standard drug group (mesalamine 500 mg/kg), G 3 as the group receiving the control beverage (without particle enrichment), and G 4 as the group receiving the enriched beverage (0.15 mL/day/rat). The study duration was 21 days. Polyphenol–protein particle‐enriched beverage ameliorated the disease activity index (DAI), weight loss, and colon weight‐to‐length ratio. Furthermore, it witnessed a reduction in ESR and CRP levels and improved hematological parameters. The following serum inflammatory markers were also decreased: TNF‐α (49.52%), IL‐1β (46.13%), and IL‐6 (55.72%). The oxidative stress was alleviated by reducing the levels of TOS (64.10%), MDA (40.71%), and MPO (83.32%) while enhancing the TAC (191.55%), GSH (47.89%), SOD (87.89%), and CAT (63.63%) levels. The polyphenol–protein‐enriched beverage was also safe in terms of its effect on liver and kidney function markers. Also, polyphenol–protein particle‐enriched beverage exerted regeneration in the histological damage in the colon. Bioaccessibility studies revealed higher total phenolic content and antioxidant activity in the digested fraction of the enriched beverage.
{"title":"Anti‐Inflammatory and Antioxidant Potential of Polyphenol–Protein Particle‐Enriched Beverage via Attenuating Biochemical Alterations and Colon Tissue Damage in Acetic Acid‐Induced Colitis in Rats","authors":"Sidra Tul‐Muntaha, Allah Rakha, Imran Pasha, Bilal Aslam","doi":"10.1002/mnfr.70295","DOIUrl":"https://doi.org/10.1002/mnfr.70295","url":null,"abstract":"This research aims to elucidate the therapeutic potential of polyphenol–protein particle‐enriched beverages in the rat model of acetic acid‐induced colitis. The 50 male Wistar rats were divided into five groups: G <jats:sub>0</jats:sub> as the negative control, G <jats:sub>1</jats:sub> as the positive control, G <jats:sub>2</jats:sub> as the standard drug group (mesalamine 500 mg/kg), G <jats:sub>3</jats:sub> as the group receiving the control beverage (without particle enrichment), and G <jats:sub>4</jats:sub> as the group receiving the enriched beverage (0.15 mL/day/rat). The study duration was 21 days. Polyphenol–protein particle‐enriched beverage ameliorated the disease activity index (DAI), weight loss, and colon weight‐to‐length ratio. Furthermore, it witnessed a reduction in ESR and CRP levels and improved hematological parameters. The following serum inflammatory markers were also decreased: TNF‐α (49.52%), IL‐1β (46.13%), and IL‐6 (55.72%). The oxidative stress was alleviated by reducing the levels of TOS (64.10%), MDA (40.71%), and MPO (83.32%) while enhancing the TAC (191.55%), GSH (47.89%), SOD (87.89%), and CAT (63.63%) levels. The polyphenol–protein‐enriched beverage was also safe in terms of its effect on liver and kidney function markers. Also, polyphenol–protein particle‐enriched beverage exerted regeneration in the histological damage in the colon. Bioaccessibility studies revealed higher total phenolic content and antioxidant activity in the digested fraction of the enriched beverage.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"79 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145396681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stem cells are the fundamental origin of fetal life, and their proliferation and differentiation are pivotal for development, health, and disease susceptibility. Emerging evidence indicates that a maternal high-fat diet (MHFD) during pregnancy and lactation disrupts the intrauterine milieu, thereby impairing stem cell development. This review synthesizes current findings on maternal HFD effects on hematopoietic stem/progenitor cells (HSPCs), neural stem cells (NSCs), adipose-derived stem cells (ASCs), osteo-progenitors, and intestinal stem cells (ISCs) in offspring. Particular emphasis is placed on molecular pathways and metabolic pathways through which maternal HFD regulates stem cell fate. Perturbations in these mechanisms, mediated by epigenetic modifications and gut microbiota dysbiosis, elevate the risk of metabolic disorders, neurodevelopmental deficits, and inflammatory diseases in progeny. Furthermore, this review highlights potential therapeutic strategies, including dietary interventions, epigenetic modulators, and microbiota-targeted therapies, that may mitigate the adverse impact of maternal HFD on stem cell programming and long-term health.
{"title":"The Impact of Maternal High-Fat Diet on Stem Cell Programming and Disease Susceptibility in Offspring","authors":"Jiahui Gao, Qinglu Tian, Siying Li, Liwei Zheng, Yachuan Zhou","doi":"10.1002/mnfr.70303","DOIUrl":"10.1002/mnfr.70303","url":null,"abstract":"<div>\u0000 \u0000 <p>Stem cells are the fundamental origin of fetal life, and their proliferation and differentiation are pivotal for development, health, and disease susceptibility. Emerging evidence indicates that a maternal high-fat diet (MHFD) during pregnancy and lactation disrupts the intrauterine milieu, thereby impairing stem cell development. This review synthesizes current findings on maternal HFD effects on hematopoietic stem/progenitor cells (HSPCs), neural stem cells (NSCs), adipose-derived stem cells (ASCs), osteo-progenitors, and intestinal stem cells (ISCs) in offspring. Particular emphasis is placed on molecular pathways and metabolic pathways through which maternal HFD regulates stem cell fate. Perturbations in these mechanisms, mediated by epigenetic modifications and gut microbiota dysbiosis, elevate the risk of metabolic disorders, neurodevelopmental deficits, and inflammatory diseases in progeny. Furthermore, this review highlights potential therapeutic strategies, including dietary interventions, epigenetic modulators, and microbiota-targeted therapies, that may mitigate the adverse impact of maternal HFD on stem cell programming and long-term health.</p>\u0000 </div>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"69 24","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145381003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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