Molecular Nutrition & Food Research最新文献_第7页

Microbial Transformation of Dietary Glycerol Contributes to Intestinal Acrolein Formation and Urinary Excretion. 膳食甘油的微生物转化有助于肠道丙烯醛的形成和尿排泄。

IF 5.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-29 DOI: 10.1002/mnfr.70289

Clarissa Schwab,Hanna Lang,Simone Stegmüller,Jiri Hosek,Angeliki Marietou,Lucia Huertas-Díaz,Qing Li,Asta Petrine Smedgaard Krings,Andrea Zander,Ulrik Kræmer Sundekilde,Elke Richling

The aldehyde acrolein has been associated with diabetes, cardiovascular, respiratory, and neurodegenerative diseases, and gut microbiota possesses the potential for acrolein release via the key enzyme glycerol/diol dehydratase (PduCDE). This study aimed at estimating the contribution of gut microbiota to endogenous acrolein production. To minimize confounding sources, we investigated the intestinal acrolein-producing potential of 20 volunteers housed under defined conditions. Glycerol was present in every meal and was detected in feces, suggesting availability to intestinal microbiota. Based on fecal metagenomics and pduC analysis, all volunteers showed potential for intestinal glycerol transformation to acrolein; the genus Anaerobutyricum was the major contributor across donors and time. Levels of urine biomarkers N-acetyl-S-(3-hydroxypropyl)-L-cysteine (3-HPMA) and N-acetyl-S-(carboxyethyl)-L-cysteine (CEMA) were higher after the consumption of meals with high glycerol levels, suggesting immediate microbial transformation to acrolein. Only a small proportion of acrolein metabolites was recovered in urine, possibly due to high compound reactivity. Donors could be separated into 3-HPMA or CEMA phenotypes based on the predominance of urine biomarkers, and phenotypes related to overall fecal microbiota and fermentation metabolite profiles. Our data show that oral fat/glycerol intake together with intestinal microbiota activity might temporarily increase endogenous acrolein formation and that urinary biomarkers link to the intestinal microbiome.

醛丙烯醛与糖尿病、心血管、呼吸和神经退行性疾病有关，肠道微生物群具有通过关键酶甘油/二醇脱水酶（PduCDE）释放丙烯醛的潜力。本研究旨在估计肠道菌群对内源性丙烯醛生产的贡献。为了尽量减少混杂源，我们调查了20名志愿者在规定条件下的肠道丙烯醛生成潜力。每顿饭中都有甘油，粪便中也检测到甘油，表明肠道微生物群可获得甘油。基于粪便宏基因组学和pduC分析，所有志愿者都显示出肠道甘油转化为丙烯醛的潜力；厌氧丁酸菌属是供体和时间上的主要供体。食用甘油含量高的食物后，尿液生物标志物n -乙酰基- s -（3-羟丙基）- l-半胱氨酸（3-HPMA）和n -乙酰基- s -（羧乙基）- l-半胱氨酸（CEMA）水平更高，表明微生物立即转化为丙烯醛。只有一小部分丙烯醛代谢物在尿液中被回收，可能是由于高的化合物反应性。根据尿液生物标志物的优势，供体可以分为3-HPMA或CEMA表型，以及与总体粪便微生物群和发酵代谢物谱相关的表型。我们的数据表明，口服脂肪/甘油摄入量与肠道微生物群活性可能会暂时增加内源性丙烯醛的形成，并且尿液生物标志物与肠道微生物群有关。

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引用次数: 0

Streptocyclin BTW is a novel member of a diverse family of circular bacteriocins in the genus Streptococcus. 链霉素BTW是一个不同的家族的环状细菌素在链球菌属的新成员。

IF 5.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-29 DOI: 10.1002/mnfr.70290

Felipe Miceli de Farias,David Hourigan,Paula M O'Connor,Colin Hill,Reynolds Paul Ross

Members of the genus Streptococcus are gram-positive bacteria, commonly found in humans and animals. This group of microorganisms has been associated with severe infections and the development of resistance to antibiotics and production of bacteriocins. Many class I bacteriocins, especially lanthipeptides, have been described for streptococci; however, only one circular bacteriocin has been described for the group (uberolysin). The objective of this study was to characterize streptocyclin BTW, a 64 amino acid circular bacteriocin produced by Streptococcus devriesei DSM 19639, as well as to assess the diversity of circular bacteriocins within the genus. Streptocyclin BTW displayed a broad inhibitory spectrum with pronounced activity against strains of streptococci. A colony mass spectrum of the strain revealed a 6118.75 Da mass, which matches the predicted mass from sequencing. The gene cluster is composed of five genes: a core peptide (stpA), a membrane protein (stpB), a DUF95 family protein (stpC), an ATP-binding protein (stpD), and a putative immunity protein (stpE). Sequence Similarity Network (SSN) analysis revealed that streptocyclin BTW-like circular bacteriocins were found in Streptococcus orisasini, Streptococcus equi, Streptococcus pneumoniae, Streptococcus pseudopneumoniae, Streptococcus mitis, and Streptococcus bouchesdurhonensis species. This is the first report of a bacteriocin produced by S. devriesei.

链球菌属的成员是革兰氏阳性细菌，常见于人类和动物。这类微生物与严重感染、对抗生素产生耐药性和产生细菌素有关。许多I类细菌素，特别是硫肽，已经被描述为链球菌；然而，只有一种圆形细菌素被描述为该群（uberolysin）。本研究的目的是表征由devriesei链球菌DSM 19639产生的64个氨基酸的环状细菌素链霉素BTW，并评估该属内环状细菌素的多样性。链霉素BTW显示出广泛的抑制谱，对链球菌菌株具有明显的活性。菌株的菌落质谱显示其质量为6118.75 Da，与测序预测的质量相符。该基因簇由5个基因组成：核心肽（stpA）、膜蛋白（stpB）、DUF95家族蛋白（stpC）、atp结合蛋白（stpD）和推定免疫蛋白（stpE）。序列相似性网络（SSN）分析结果显示，在奥里氏链球菌、马链球菌、肺炎链球菌、假肺炎链球菌、炎链球菌和布切斯杜洪氏链球菌中均发现了btw样环状细菌素。这是首次报道由S. devriesei产生的细菌素。

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引用次数: 0

Modulation of Intestinal Microbiota Through Kefir-Based Beverages in a Murine Model of Acute Pancreatitis. 开菲尔饮料对小鼠急性胰腺炎模型中肠道微生物群的调节作用。

IF 5.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-29 DOI: 10.1002/mnfr.70304

Diego Aguirre-Ramírez,María Garrido,Lourdes Sánchez,Laura Grasa

Acute pancreatitis (AP) severely disrupts gut microbiota, leading to dysbiosis. This study investigated whether kefir derived from pasteurized whole milk (PMK) or whey (WK) could mitigate AP-induced gut microbiota alterations and promote gut health in a murine model. Male BALB/cJRj mice were assigned to CONTROL, AP-induced (PAN), PMK, WK, PMKPAN, or WKPAN groups. Fecal bacterial DNA was analyzed via 16S rRNA sequencing. AP induction caused characteristic dysbiosis, increasing Marinifilaceae, Tannerellaceae, Odoribacter, and Parabacteroides. In AP mice, PMK treatment significantly reduced Tannerellaceae and Parabacteroides, while increasing potentially anti-inflammatory Marinifilaceae and butyrate-producing Odoribacter. In healthy mice, PMK reduced pro inflammatory Desulfovibrionaceae and Bilophila, enriching short-chain fatty acid (SCFA) producers like Acetatifactor and Roseburia, with pathway analysis showing increased acetate kinase activity. WK treatment in healthy animals also promoted SCFA-producers (Acetatifactor, Odoribacter) and Muribaculaceae. In the AP model, WK upregulated bacteria involved in fatty acid biosynthesis pathways. Dairy-based kefir, particularly PMK, demonstrates a significant capacity to counteract AP-induced gut microbiota imbalances. These findings highlight kefir's nutritional relevance as a functional food with potential preventive and therapeutic applications for supporting gut health in the context of AP.

急性胰腺炎（AP）严重破坏肠道微生物群，导致生态失调。本研究在小鼠模型中研究了从巴氏全脂牛奶（PMK）或乳清（WK）中提取的开菲尔是否可以减轻ap诱导的肠道微生物群改变并促进肠道健康。雄性BALB/cJRj小鼠分为CONTROL组、ap诱导（PAN）组、PMK组、WK组、PMKPAN组和WKPAN组。通过16S rRNA测序分析粪便细菌DNA。AP诱导引起特征性生态失调，增加了Marinifilaceae、Tannerellaceae、Odoribacter和Parabacteroides。在AP小鼠中，PMK处理显著减少了鞣酸科和拟副杆菌，同时增加了潜在的抗炎Marinifilaceae和产生丁酸盐的臭杆菌。在健康小鼠中，PMK降低了促炎的Desulfovibrionaceae和Bilophila，丰富了短链脂肪酸（SCFA）生产者，如Acetatifactor和Roseburia，通路分析显示醋酸激酶活性增加。健康动物的WK治疗也促进了scfa产生者（乙酰化因子，气味杆菌）和Muribaculaceae。在AP模型中，WK上调了参与脂肪酸生物合成途径的细菌。以乳制品为基础的开菲尔，特别是PMK，显示出对抗ap诱导的肠道微生物群失衡的显着能力。这些发现强调了开菲尔作为一种功能性食品的营养相关性，在AP背景下具有潜在的预防和治疗应用，可支持肠道健康。

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引用次数: 0

Benefits of Nutrients and Phytonutrients in Nuts and Legumes on Platelet Aggregation Through DNA Methylation. 坚果和豆类中的营养素和植物营养素通过DNA甲基化对血小板聚集的益处。

IF 5.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-27 DOI: 10.1002/mnfr.70297

Siwaphorn Chaimati,Jintana Sirivarasai,Nareerat Sutjarit

Platelets play a central role in hemostasis and thrombosis, but their hyperactivity is closely associated with an increased risk of cardiovascular diseases (CVDs). Appearing evidence emphasizes the importance of plant-based diets, especially those rich in nuts and legumes, which are associated with modulating platelet function and reducing CVD risks. Nuts and legumes are abundant sources of nutrients, including mono/polyunsaturated fatty acids, fiber, vitamins, minerals, and bioactive compounds, such as polyphenols, flavonoids, and phenolic acids. These components, both nutrients and bioactive compounds, were reported to have antiplatelet aggregation properties. Furthermore, epigenetic mechanisms, particularly DNA methylation, regulate gene expression, including genes involved in platelet activation pathways, such as platelet endothelial aggregation receptor 1 (PEAR1), which are related to nutrients such as folate and methionine, contribute to one-carbon metabolism, and bioactive compounds promote methylation patterns related to DNA methylation enzymes and reduced platelet hyperactivity. So, this review summarizes current findings and explores the potential association between nuts and legumes consumption and platelet aggregation (PA) through DNA methylation, emphasizing their role in cardiovascular health and the potential for targeted dietary interventions.

血小板在止血和血栓形成中起着核心作用，但其过度活跃与心血管疾病（cvd）的风险增加密切相关。新出现的证据强调了植物性饮食的重要性，特别是那些富含坚果和豆类的饮食，它们与调节血小板功能和降低心血管疾病风险有关。坚果和豆类是丰富的营养来源，包括单/多不饱和脂肪酸、纤维、维生素、矿物质和生物活性化合物，如多酚、类黄酮和酚酸。据报道，这些成分，包括营养成分和生物活性化合物，具有抗血小板聚集特性。此外，表观遗传机制，特别是DNA甲基化，调节基因表达，包括参与血小板活化途径的基因，如血小板内皮聚集受体1 (PEAR1)，它与叶酸和蛋氨酸等营养物质有关，有助于单碳代谢，生物活性化合物促进与DNA甲基化酶相关的甲基化模式和减少血小板过度活跃。因此，本文总结了目前的研究结果，并通过DNA甲基化探讨了坚果和豆类消费与血小板聚集（PA）之间的潜在关联，强调了它们在心血管健康中的作用以及有针对性的饮食干预的潜力。

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引用次数: 0

Dietary Gallic Acid Alleviates Hypercholesterolemia in High-Fat-Diet-Fed Mice by Modulating Cholesterol and Bile Acid Metabolism. 膳食没食子酸通过调节胆固醇和胆汁酸代谢减轻高脂肪饮食喂养小鼠的高胆固醇血症。

IF 5.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-23 DOI: 10.1002/mnfr.70291

Yu Wang,Juan Sun,Lamei Xue,Yujie Sun,Kuiliang Zhang,Mingcong Fan,Haifeng Qian,Yan Li,Li Wang

Hypercholesterolemia is a major risk factor for cardiovascular diseases. While gallic acid, a natural phenolic compound, shows hypolipidemic effects, its mechanisms remain elusive. This study investigated gallic acid's modulation of cholesterol and bile acid metabolism in high-fat diet (HFD)-induced hypercholesterolemia. Mice on an HFD were supplemented with gallic acid for 12 weeks. This study systematically examined gallic acid's modulation of cholesterol metabolism (synthesis, uptake, efflux) and enterohepatic bile acid circulation. The results indicated that gallic acid protected mice against diet-induced hypercholesterolemia and hepatic steatosis. Notably, gallic acid intervention favorably altered the dyslipidemic profile by increasing HDL-C while decreasing atherogenic lipids (TG, TC, and LDL-C) in HFD-fed mice. Mechanistically, gallic acid reduced cholesterol accumulation by modulating FXR-mediated liver-gut crosstalk of bile acids, significantly suppressing both ileal and hepatic FXR expression in HFD-fed mice. This inhibition downregulated key FXR target genes (ileal FGF15/SHP/I-BABP/ASBT and hepatic SHP/MAFG), reducing bile acid reabsorption while enhancing hepatic synthesis. Moreover, gallic acid activated Nrf2/HO-1 signaling and promoted antioxidant capacity in HFD-fed mice. Dietary gallic acid alleviates diet-induced hypercholesterolemia and hepatic steatosis, highlighting its potential as a safe and effective dietary supplement for managing hyperlipidemia and preventing metabolic liver disease.

高胆固醇血症是心血管疾病的主要危险因素。没食子酸是一种天然的酚类化合物，具有降血脂作用，但其作用机制尚不明确。本研究探讨了没食子酸在高脂肪饮食（HFD）诱导的高胆固醇血症中对胆固醇和胆汁酸代谢的调节作用。小鼠在HFD中补充没食子酸12周。本研究系统地考察了没食子酸对胆固醇代谢（合成、摄取、排出）和肝肠胆汁酸循环的调节。结果表明没食子酸对小鼠抗饮食性高胆固醇血症和肝脂肪变性具有保护作用。值得注意的是，没食子酸干预通过增加hfd喂养小鼠的HDL-C，同时降低致动脉粥样硬化脂质（TG， TC和LDL-C），有利于改变血脂异常状况。在机制上，没食子酸通过调节FXR介导的胆汁酸肝肠串扰来降低胆固醇积累，显著抑制hfd喂养小鼠回肠和肝脏FXR的表达。这种抑制作用下调了FXR关键靶基因（回肠FGF15/SHP/I-BABP/ASBT和肝脏SHP/MAFG），减少了胆汁酸的重吸收，同时增强了肝脏合成。此外，没食子酸激活Nrf2/HO-1信号，提高hfd喂养小鼠的抗氧化能力。膳食没食子酸可减轻饮食引起的高胆固醇血症和肝脂肪变性，突出其作为一种安全有效的膳食补充剂的潜力，用于管理高脂血症和预防代谢性肝病。

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引用次数: 0

Front Cover: Folic Acid Reduces Insulin Resistance in Mice With Diet-Induced Obesity by Altering One-Carbon Metabolism and DNA Methylation Patterns of Hypothalamic and Hepatic Insulin Receptor Gene 封面：叶酸通过改变下丘脑和肝脏胰岛素受体基因的单碳代谢和DNA甲基化模式，降低饮食诱导肥胖小鼠的胰岛素抵抗

IF 4.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-21 DOI: 10.1002/mnfr.70294

Eva Kranenburg, Ruslan Kubant, Clara E. Cho, Zeyu Yang, Mahaylia Datars, Jianzhang Dong, G. Harvey Anderson

The cover image is based on the article Folic Acid Reduces Insulin Resistance in Mice With Diet-Induced Obesity by Altering One-Carbon Metabolism and DNA Methylation Patterns of Hypothalamic and Hepatic Insulin Receptor Gene by Harvey Anderson et al., https://doi.org/10.1002/mnfr.70181

封面图片基于Harvey Anderson等人的文章《叶酸通过改变下丘脑和肝脏胰岛素受体基因的单碳代谢和DNA甲基化模式，降低饮食诱导肥胖小鼠的胰岛素抵抗》，https://doi.org/10.1002/mnfr.70181

引用次数: 0

Issue Information: Mol. Nutr. Food Res. 20'25 发布信息：Mol. Nutr。Food Res. 25

IF 4.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-21 DOI: 10.1002/mnfr.70292

引用次数: 0

Bacteriocinogenic Pediococcus acidilactici ST41VF Isolated From Bulgaria Feta Cheese: Probiotic Potential, Biopreservative Properties, and Safety Evaluation 保加利亚菲达奶酪中产菌性酸碱小球菌ST41VF的分离：益生菌潜力、生物保存特性及安全性评价

IF 4.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-20 DOI: 10.1002/mnfr.70268

Vitor Luis Fagundes, Marcos Vinício Alves, Svetoslav Dimitrov Todorov, Julia Arantes Galvão

Dairy products are traditional sources of potentially bactriocinogenic and probiotic microorganisms, especially lactic acid bacteria (LAB). This study aimed to isolate bacteriocin-producing LAB from Bulgarian feta cheese and assess their biopreservative, probiotic potential, and safety. Pediococcus acidilactici ST41VF was isolated and shown to inhibit multiple Listeria monocytogenes strains through the production of bacteriocin. This bacteriocin remained stable across a range of pH levels, temperatures, and chemical exposures relevant to pharmaceutical contexts. The highest bacteriocin concentration (6400 AU/mL) occurred during the stationary growth phase. Safety assessments revealed that ST41VF does not produce biogenic amines or gelatinase, lacks proteolytic and mucin-degrading activity, and exhibits γ-hemolytic behavior. It tolerated gastrointestinal simulations, thriving in various pH levels, salt concentrations, and ox-bile. The strain produced 4.998 g/L of d-lactic acid and 7.110 g/L of l-lactic acid, a relevant information regarding potential negative effects of d-lacid acid. Considering its robust antimicrobial activity, resilience, and safety profile, P. acidilactici ST41VF demonstrates strong potential as a biopreservative in food systems and a probiotic candidate for therapeutic applications.

乳制品是潜在的致病菌和益生菌微生物的传统来源，尤其是乳酸菌（LAB）。本研究旨在从保加利亚羊奶酪中分离产生细菌素的乳酸菌，并评估其生物保存性、益生菌潜力和安全性。分离出嗜酸性Pediococcus aciliacactii ST41VF，并通过产生细菌素抑制多种单核增生李斯特菌菌株。这种细菌素在一系列pH值、温度和与药物环境相关的化学暴露中保持稳定。细菌素的最高浓度为6400 AU/mL。安全性评估显示，ST41VF不产生生物胺或明胶酶，缺乏蛋白水解和黏液降解活性，并表现出γ-溶血行为。它能耐受胃肠道模拟，在各种pH值、盐浓度和胆汁中都能茁壮成长。菌株产生的d-乳酸为4.998 g/L， L -乳酸为7.110 g/L，这是d-乳酸潜在负面影响的相关信息。考虑到其强大的抗菌活性、弹性和安全性，P. acidilactici ST41VF作为食品系统中的生物防腐剂和治疗应用的候选益生菌具有强大的潜力。

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引用次数: 0

Microbiome and Metabolome Alterations in Nrf2 Knockout Mice With Induced Gut Inflammation and Fed With Phenethyl Isothiocyanate and Cranberry Enriched Diets 异硫氰酸苯乙酯和富含蔓越莓的饲粮对Nrf2敲除小鼠肠道炎症的影响

IF 4.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-12 DOI: 10.1002/mnfr.70283

Ran Yin, Davit Sargsyan, Renyi Wu, Rasika Hudlikar, Shanyi Li, Hsiao-Chen Kuo, Md Shahid Sarwar, Yuyin Zhou, Zhan Gao, Amy Howell, Chi Chen, Martin J. Blaser, Ah-Ng Kong

Cranberries contain phytochemicals with potent antioxidant properties. Phenethyl isothiocyanate (PEITC) is abundant in crucifers and possesses anti-cancer and anti-inflammatory properties. These food additives can alter gut microbiota and improve the host's health. Microbiome and microbial metabolome interactions with the host's cells help maintain gastrointestinal (GI) tract homeostasis. Cranberry and PEITC enriched diets were fed to wild-type (WT) and Nrf2 knockout (KO) mice, including those challenged with dextran sulfate sodium (DSS), and their gut microbiomes and metabolomes were examined. Relative abundances of Deferribacteres, Epsilonbacteraeota, and Proteobacteria decreased, while Firmicutes and Verrucomicrobia increased in the DSS-challenged mice samples. These trends were reversed by PEITC and cranberry enriched diets. The diets also preserved the Firmicutes-to-Bacteroidetes ratio, an endpoint associated with gut inflammation and obesity. DSS challenge altered production of several metabolites. Nrf2 KO mice samples had lower concentrations of short-chain fatty acids (SCFA) and amino acids, and higher concentrations of secondary bile acids.Nrf2 KO mice microbiomes exhibited higher richness and diversity. PEITC and cranberry enriched diets positively affected hosts’ microbiomes and boosted several microbial metabolites. Phenotypic expression of Nrf2 impacted the microbiota and metabolic reprogramming induced by DSS-mediated inflammation and dietary supplements of cranberry and PEITC.

蔓越莓含有具有有效抗氧化特性的植物化学物质。异硫氰酸苯乙酯（PEITC）富含十字花科植物，具有抗癌和抗炎作用。这些食品添加剂可以改变肠道菌群，改善宿主的健康。微生物组和微生物代谢组与宿主细胞的相互作用有助于维持胃肠道稳态。将蔓越莓和PEITC富集饲料饲喂野生型（WT）和Nrf2敲除（KO）小鼠，包括dextran sulfate sodium （DSS）小鼠，并检测其肠道微生物组和代谢组。在dss挑战小鼠样本中，脱铁菌、Epsilonbacteraeota和变形菌门的相对丰度下降，而厚壁菌门和Verrucomicrobia的相对丰度增加。这些趋势被PEITC和富含蔓越莓的饮食所逆转。饮食也保持了厚壁菌与拟杆菌的比例，这是与肠道炎症和肥胖相关的一个终点。DSS挑战改变了几种代谢物的产生。Nrf2 KO小鼠样品的短链脂肪酸（SCFA）和氨基酸浓度较低，次级胆汁酸浓度较高。Nrf2 KO小鼠微生物组具有较高的丰富度和多样性。富含PEITC和蔓越莓的饮食对宿主的微生物群产生积极影响，并提高了几种微生物代谢物。Nrf2的表型表达影响dss介导的炎症和膳食补充蔓越莓和PEITC诱导的微生物群和代谢重编程。

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引用次数: 0

Peptides From Adzuki Bean and Soybean Improved Insulin-AKT Signaling-Related Pathways in Healthy and Insulin-Resistant States in Human Liver Cells. 小豆和大豆肽改善人肝细胞健康和胰岛素抵抗状态下胰岛素- akt信号相关通路

IF 5.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research

Pub Date : 2025-10-09 DOI: 10.1002/mnfr.70285

Shu Hang Kwan,Elvira Gonzalez de Mejia

The objectives were to determine the effect and mechanism of functional peptides identified from digested adzuki bean β-vignin and soybean β-conglycinin on insulin-AKT signaling and hepatic glucose uptake markers and to investigate the roles of these peptides in modulating the insulin-AKT signaling pathway in human liver cells in healthy and insulin-resistant states. Methods and Results: Adzuki bean β-vignin and soybean β-conglycinin proteins were isolated and digested using simulated gastrointestinal digestion. Peptide sequences (VP, PM, FNE, LLS, and IPA), in silico analysis and in vitro (cell-free and HepG2 cell-based) systems confirmed their safety and dipeptidyl peptidase IV (DPP IV) inhibitory capacity. IPA and VP showed high intestinal absorption and low toxicity, inhibited DPP IV (IPA IC50, 7.86 µM; VP IC50, 9.58 µM). Microarray results showed that VP (9.58 µM) stimulated the insulin signaling pathway in the healthy state. In healthy and insulin-resistant states, VP (9.58 µM) and IPA (7.86 µM) significantly increased (p < 0.05) protein expression of IRS-1, Akt-1, and Glut 2, suggesting their potential in modulating insulin signaling and hepatic glucose uptake. Peptides exhibited antidiabetic properties by stimulating insulin signaling. These in vitro findings support further investigation into their application in functional food ingredients targeting glucose metabolism.

目的是确定从消化小豆中鉴定的β-vignin和大豆β-conglycin功能肽对胰岛素- akt信号通路和肝脏葡萄糖摄取标志物的作用和机制，并研究这些肽在调节健康和胰岛素抵抗状态下人类肝细胞胰岛素- akt信号通路中的作用。方法与结果：分离小豆β-vignin蛋白和大豆β-conglycin蛋白，采用模拟胃肠消化法进行消化。肽序列（VP， PM， FNE， LLS和IPA），硅分析和体外（无细胞和基于HepG2细胞）系统证实了它们的安全性和二肽基肽酶IV （DPP IV）抑制能力。IPA和VP表现出高肠道吸收、低毒性，对DPP IV具有抑制作用（IPA IC50, 7.86µM; VP IC50, 9.58µM）。微阵列结果显示，在健康状态下，VP（9.58µM）可刺激胰岛素信号通路。在健康和胰岛素抵抗状态下，VP（9.58µM）和IPA（7.86µM）显著增加IRS-1、Akt-1和Glut - 2蛋白的表达（p < 0.05），提示它们可能调节胰岛素信号和肝脏葡萄糖摄取。多肽通过刺激胰岛素信号传导表现出抗糖尿病的特性。这些体外研究结果支持进一步研究其在针对葡萄糖代谢的功能性食品成分中的应用。

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引用次数: 0