Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.34
S. Nazeer
Brackish water used for irrigation in shortage of appropriate soil-water-crop practices often constitutes salinity in the soil profile. Canal irrigation water is scarce to aid agriculture; thus, a supplementary water supply requires accessibility from drainage water. In Pakistan, groundwater is brackish because of elevated levels of electrical conductivity (EC), residual sodium carbonates (RSC), and sodium adsorption ratio (SAR). But these waters can benefit well for irrigation during the primary phase of saline-sodic soil’s reclamation, if employing appropriate management practices, such as, chemical and organic amendments. A pot trial procedure ran under environmental conditions at the research area of the Department of Soil and Environmental Sciences, College of Agriculture, University of Sargodha, to assess the effect of sodic water with various amendments on sorghum and berseem fodder crops. The pot experiment comprised seven treatments, including T1 = Control having canal water with SAR 0.1 and EC 0.2 dS m-1 ; T2 = Sodic water with SAR 15; T3 = Sodic water (SAR 15) + Gypsum; T4 = Sodic water (SAR 15) + H2SO4; T5 = Sodic water (SAR 15) + compost; T6 = Sodic water (SAR 15) + FYM; T7 = Sodic water (SAR 15) + poultry manure, with three replicates under complete randomized design (CRD) by sowing sorghum “JS-88” and berseem “Hisar Berseem 1” cultivars taken from the Fodder Research Institute (FRI), Sargodha. The agronomic and fodder quality attributes were maximum in T3 treatment in sorghum compared with other concentrations and berseem. In both crops, mineral nutrients were variable, and nitrogen, phosphorus, and potassium were maximum in T5, compared with others.
{"title":"EXTENUATING THE DETRIMENTAL EFFECTS OF SODIC WATER ON AGRONOMIC, IONIC, AND QUALITY ATTRIBUTES OF FODDERS","authors":"S. Nazeer","doi":"10.54910/sabrao2023.55.4.34","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.34","url":null,"abstract":"Brackish water used for irrigation in shortage of appropriate soil-water-crop practices often constitutes salinity in the soil profile. Canal irrigation water is scarce to aid agriculture; thus, a supplementary water supply requires accessibility from drainage water. In Pakistan, groundwater is brackish because of elevated levels of electrical conductivity (EC), residual sodium carbonates (RSC), and sodium adsorption ratio (SAR). But these waters can benefit well for irrigation during the primary phase of saline-sodic soil’s reclamation, if employing appropriate management practices, such as, chemical and organic amendments. A pot trial procedure ran under environmental conditions at the research area of the Department of Soil and Environmental Sciences, College of Agriculture, University of Sargodha, to assess the effect of sodic water with various amendments on sorghum and berseem fodder crops. The pot experiment comprised seven treatments, including T1 = Control having canal water with SAR 0.1 and EC 0.2 dS m-1 ; T2 = Sodic water with SAR 15; T3 = Sodic water (SAR 15) + Gypsum; T4 = Sodic water (SAR 15) + H2SO4; T5 = Sodic water (SAR 15) + compost; T6 = Sodic water (SAR 15) + FYM; T7 = Sodic water (SAR 15) + poultry manure, with three replicates under complete randomized design (CRD) by sowing sorghum “JS-88” and berseem “Hisar Berseem 1” cultivars taken from the Fodder Research Institute (FRI), Sargodha. The agronomic and fodder quality attributes were maximum in T3 treatment in sorghum compared with other concentrations and berseem. In both crops, mineral nutrients were variable, and nitrogen, phosphorus, and potassium were maximum in T5, compared with others.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44746264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.35
E. Rashid, Z. Hussain, M. A. Nawaz, S. Rashid, Prof. Naqib Ullah, Khan
Pea (Pisum sativum L.), a highly nutritious vegetable, is extremely sensitive to salt stress conditions. A pot study evaluated four pea genotypes (Samrina Zard, Climax, Ambassador, and Green Arrow) by exposing them to control, 2.5, 5.0, and 7.0 dS m-1 by applying NaCl, Na2SO4, MgSO4, and CaCl2 salts. The pots under a completely randomized design (CRD) layout had four replications. Immense genetic variations occurred among the pea genotypes under salt stress. Samrina Zard showed better physiological (transpiration and photosynthesis rates, stomatal conductance, water use efficiency, and chlorophyll) and morphological traits (shoot/root length, shoot/root dry weight, number of leaves per plant, and leaf area). Genotype Samrina Zard significantly maintained the highest percentage of shoot length (14.54%), root length (28.28%), shoot dry weight (19.58%), root dry weight (36.36%), number of leaves (27.24%), and leaf area (21.59%) at a higher level of salinity 7 dS m-1 compared with the control and all other treatments. In contrast, the Ambassador genotype was categorically salt-sensitive based on the least percentage increase in shoot length (22.42%), dry weight of shoot (67.57%), dry weight of root (59.59%), number of leaves (47.69%) and leaf area (23.72%). However, salinity reduced the physiological attributes in both genotypes. Regardless of salt treatments, Samrina Zard performed better than Ambassador regarding photosynthesis (48.07%), transpiration rate (18.76%), stomatal conductance (45.42%), water use efficiency (55.88%), and chlorophyll contents (29.44%). According to study findings, Samrina Zard performed best against salinity stress.
{"title":"EVALUATION OF PEA GENOTYPES FOR SALT STRESS TOLERANCE","authors":"E. Rashid, Z. Hussain, M. A. Nawaz, S. Rashid, Prof. Naqib Ullah, Khan","doi":"10.54910/sabrao2023.55.4.35","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.35","url":null,"abstract":"Pea (Pisum sativum L.), a highly nutritious vegetable, is extremely sensitive to salt stress conditions. A pot study evaluated four pea genotypes (Samrina Zard, Climax, Ambassador, and Green Arrow) by exposing them to control, 2.5, 5.0, and 7.0 dS m-1 by applying NaCl, Na2SO4, MgSO4, and CaCl2 salts. The pots under a completely randomized design (CRD) layout had four replications. Immense genetic variations occurred among the pea genotypes under salt stress. Samrina Zard showed better physiological (transpiration and photosynthesis rates, stomatal conductance, water use efficiency, and chlorophyll) and morphological traits (shoot/root length, shoot/root dry weight, number of leaves per plant, and leaf area). Genotype Samrina Zard significantly maintained the highest percentage of shoot length (14.54%), root length (28.28%), shoot dry weight (19.58%), root dry weight (36.36%), number of leaves (27.24%), and leaf area (21.59%) at a higher level of salinity 7 dS m-1 compared with the control and all other treatments. In contrast, the Ambassador genotype was categorically salt-sensitive based on the least percentage increase in shoot length (22.42%), dry weight of shoot (67.57%), dry weight of root (59.59%), number of leaves (47.69%) and leaf area (23.72%). However, salinity reduced the physiological attributes in both genotypes. Regardless of salt treatments, Samrina Zard performed better than Ambassador regarding photosynthesis (48.07%), transpiration rate (18.76%), stomatal conductance (45.42%), water use efficiency (55.88%), and chlorophyll contents (29.44%). According to study findings, Samrina Zard performed best against salinity stress.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49633441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.24
S. S. Alaarage, A. Alamery, Dr. Osama Osman Atallah
A field experiment on sorghum (Sorghum bicolor L.) with diverse levels of fertilization, carried out in the spring of 2022 in a randomized complete block design (RCBD) with factorial arrangement, had three replications and two factors at the field of Ibn-Al-Bitar Vocational Preparatory School, District AlHussainiya, Kerbala, Iraq. The first factor comprised three sorghum cultivars (V1 = Al-Khair, V2 = Rabeh, and V3 = Bohuth-70), while the second was combinations of nitrogen and bio-fertilizers (F0 = recommended dose of N 320 kg ha-1 , F1 = 3/4 of the recommended amount of N with Azotobacter, F2 = 1/2 of the recommended dosage of N + Aotobacter, F3 = 1/4 of the recommended dose of N + Azotobacter, and F4 = Azotobacter + No nitrogen fertilizer). The results showed that the sorghum cultivar Bohuth-70 was significantly superior for enhanced plant height (204.63 cm), number of leaves plant-1 (9.807), 1000-grain weight (35.27 g), and the grain yield (6.192 Mg.ha-1 ). However, cultivar Al-Khair produced the highest mean leaf area (4,929 cm2 ) and grains per head (4,428 grains.head-1 ). Cultivar Rabeh excelled in performance, with the highest mean value for stem diameter (24.67 mm) and head length (31.29 cm). The F0 treatment recommended dose of N 320 kg ha-1 was considerably superior in the number of leaves (9.867 leaves plant-1 ), stem diameter (24.8 mm), and grain yield (6.145 Mg.ha-1 ). The F1 treatment of 3/4 of the recommended dose of N with Aotobacter excelled in plant height (166.57 cm), leaf area (4,936 cm2 ), and number of grains (3,916 grain head-1 ). However, the F2 treatment 1/2 of the recommended dose of N + Aotobacter was notably superior in head length (30.18 cm) and 1000-grain weight (29.93 grains). As for the interaction, the sorghum cultivar Bohuth70 with F0 treatment excelled in the number of leaves plant-1 (10.33 leaves) and the grain yield (7.157 Mg.ha-1 ), while the cultivar Al-Khair with F1 treatment excelled in stem diameter (26.70 mm) and leaf area (5,164 cm2 ). A field experiment on sorghum (Sorghum bicolor L.) with diverse levels of fertilization, carried out in the spring of 2022 in a randomized complete block design (RCBD) with factorial arrangement, had three replications and two factors at the field of Ibn-Al-Bitar Vocational Preparatory School, District AlHussainiya, Kerbala, Iraq. The first factor comprised three sorghum cultivars (V1 = Al-Khair, V2 = Rabeh, and V3 = Bohuth-70), while the second was combinations of nitrogen and bio-fertilizers (F0 = recommended dose of N 320 kg ha-1 , F1 = 3/4 of the recommended amount of N with Azotobacter, F2 = 1/2 of the recommended dosage of N + Aotobacter, F3 = 1/4 of the recommended dose of N + Azotobacter, and F4 = Azotobacter + No nitrogen fertilizer). The results showed that the sorghum cultivar Bohuth-70 was significantly superior for enhanced plant height (204.63 cm), number of leaves plant-1 (9.807), 1000-grain weight (35.27 g), and the grain yield (6.192 Mg.ha-1 ). However,
2022年春季，在伊拉克克尔巴拉Al-Hussainiya区Ibn Al-Bitar职业预备学校的田地里，采用因子排列的随机完全区组设计（RCBD）进行了一项不同施肥水平的高粱田间试验，共有三个重复和两个因子。第一个因素包括三个高粱品种（V1=Al-Khair，V2=Rabeh和V3=Bohuth-70），第二种是氮和生物肥料的组合（F0=320 kg ha-1的推荐剂量，F1=3/4的氮与固氮菌推荐量，F2=1/2的N+固氮菌推荐剂量，F3=1/4的N+硝化菌推荐剂量和F4=固氮菌+无氮肥）。结果表明，高粱品种博胡特70的株高（204.63cm）、单株叶数-1（9.807）、千粒重（35.27g）和籽粒产量（6.192Mg.ha-1）均显著提高。然而，栽培品种Al Khair的平均叶面积（4929平方厘米）和人均粒数（4428粒/头-1）最高。品种Rabeh表现优异，茎粗（24.67mm）和头长（31.29cm）的平均值最高。F0处理推荐剂量N 320 kg ha-1在叶数（9.867叶-株-1）、茎径（24.8 mm）和籽粒产量（6.145 Mg.ha-1）方面显著优于F0处理。在株高（166.57cm）、叶面积（4936cm2）和粒数（3916粒头-1）方面，用Aotobacter处理推荐剂量的3/4的N的F1处理表现优异。然而，推荐剂量N+Aotobacter的1/2的F2处理在头长（30.18cm）和1000粒重（29.93粒）方面显著优越。在交互作用方面，F0处理的高粱品种Bohuth70的单株叶数（10.33片）和籽粒产量（7.157 Mg.ha-1）表现优异，而F1处理的品种Al Khair的茎径（26.70 mm）和叶面积（5164 cm2）表现优异。2022年春季，在伊拉克克尔巴拉Al-Hussainiya区Ibn Al-Bitar职业预备学校的田地里，采用因子排列的随机完全区组设计（RCBD）进行了一项不同施肥水平的高粱田间试验，共有三个重复和两个因子。第一个因素包括三个高粱品种（V1=Al-Khair，V2=Rabeh和V3=Bohuth-70），第二种是氮和生物肥料的组合（F0=320 kg ha-1的推荐剂量，F1=3/4的氮与固氮菌推荐量，F2=1/2的N+固氮菌推荐剂量，F3=1/4的N+硝化菌推荐剂量和F4=固氮菌+无氮肥）。结果表明，高粱品种博胡特70的株高（204.63cm）、单株叶数-1（9.807）、千粒重（35.27g）和籽粒产量（6.192Mg.ha-1）均显著提高。然而，栽培品种Al Khair的平均叶面积（4929平方厘米）和人均粒数（4428粒/头-1）最高。品种Rabeh表现优异，茎粗（24.67mm）和头长（31.29cm）的平均值最高。F0处理推荐剂量N 320 kg ha-1在叶数（9.867叶-株-1）、茎径（24.8 mm）和籽粒产量（6.145 Mg.ha-1）方面显著优于F0处理。在株高（166.57cm）、叶面积（4936cm2）和粒数（3916粒头-1）方面，用Aotobacter处理推荐剂量的3/4的N的F1处理表现优异。然而，推荐剂量N+Aotobacter的1/2的F2处理在头长（30.18cm）和1000粒重（29.93粒）方面显著优越。在交互作用方面，F0处理的高粱品种Bohuth70的单株叶数（10.33片）和籽粒产量（7.157 Mg.ha-1）表现优异，而F1处理的品种Al Khair的茎径（26.70 mm）和叶面积（5164 cm2）表现优异。
{"title":"ROLE OF BIO-AZOTOBACTER AND NITROGEN FERTILIZERS ON GROWTH AND YIELD TRAITS OF SORGHUM (SORGHUM BICOLOR L.)","authors":"S. S. Alaarage, A. Alamery, Dr. Osama Osman Atallah","doi":"10.54910/sabrao2023.55.4.24","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.24","url":null,"abstract":"A field experiment on sorghum (Sorghum bicolor L.) with diverse levels of fertilization, carried out in the spring of 2022 in a randomized complete block design (RCBD) with factorial arrangement, had three replications and two factors at the field of Ibn-Al-Bitar Vocational Preparatory School, District AlHussainiya, Kerbala, Iraq. The first factor comprised three sorghum cultivars (V1 = Al-Khair, V2 = Rabeh, and V3 = Bohuth-70), while the second was combinations of nitrogen and bio-fertilizers (F0 = recommended dose of N 320 kg ha-1 , F1 = 3/4 of the recommended amount of N with Azotobacter, F2 = 1/2 of the recommended dosage of N + Aotobacter, F3 = 1/4 of the recommended dose of N + Azotobacter, and F4 = Azotobacter + No nitrogen fertilizer). The results showed that the sorghum cultivar Bohuth-70 was significantly superior for enhanced plant height (204.63 cm), number of leaves plant-1 (9.807), 1000-grain weight (35.27 g), and the grain yield (6.192 Mg.ha-1 ). However, cultivar Al-Khair produced the highest mean leaf area (4,929 cm2 ) and grains per head (4,428 grains.head-1 ). Cultivar Rabeh excelled in performance, with the highest mean value for stem diameter (24.67 mm) and head length (31.29 cm). The F0 treatment recommended dose of N 320 kg ha-1 was considerably superior in the number of leaves (9.867 leaves plant-1 ), stem diameter (24.8 mm), and grain yield (6.145 Mg.ha-1 ). The F1 treatment of 3/4 of the recommended dose of N with Aotobacter excelled in plant height (166.57 cm), leaf area (4,936 cm2 ), and number of grains (3,916 grain head-1 ). However, the F2 treatment 1/2 of the recommended dose of N + Aotobacter was notably superior in head length (30.18 cm) and 1000-grain weight (29.93 grains). As for the interaction, the sorghum cultivar Bohuth70 with F0 treatment excelled in the number of leaves plant-1 (10.33 leaves) and the grain yield (7.157 Mg.ha-1 ), while the cultivar Al-Khair with F1 treatment excelled in stem diameter (26.70 mm) and leaf area (5,164 cm2 ). A field experiment on sorghum (Sorghum bicolor L.) with diverse levels of fertilization, carried out in the spring of 2022 in a randomized complete block design (RCBD) with factorial arrangement, had three replications and two factors at the field of Ibn-Al-Bitar Vocational Preparatory School, District AlHussainiya, Kerbala, Iraq. The first factor comprised three sorghum cultivars (V1 = Al-Khair, V2 = Rabeh, and V3 = Bohuth-70), while the second was combinations of nitrogen and bio-fertilizers (F0 = recommended dose of N 320 kg ha-1 , F1 = 3/4 of the recommended amount of N with Azotobacter, F2 = 1/2 of the recommended dosage of N + Aotobacter, F3 = 1/4 of the recommended dose of N + Azotobacter, and F4 = Azotobacter + No nitrogen fertilizer). The results showed that the sorghum cultivar Bohuth-70 was significantly superior for enhanced plant height (204.63 cm), number of leaves plant-1 (9.807), 1000-grain weight (35.27 g), and the grain yield (6.192 Mg.ha-1 ). However, ","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46693516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.13
A. N. Laily, B. S. Daryono, A. Purwantoro, Prof. Zahoor Ahmed, Soomro
Highland papaya (Vasconcellea pubescens A.DC.) is a monoecious and dioecious plant with female and male organs. In Indonesia, three sexes of highland papaya exist with different telomere lengths, which protect chromosomes and deoxyribonucleic acid (DNA) from damage. Therefore, the purposeful study aimed to determine the telomere length, elemental composition, and phytochemical content of highland papaya leaves belonging to their different sexes. The telomere length observed and studied in said leaves (female, male, and monoecious) used the polymerase chain reaction (PCR) procedure. A scanning electron microscopy (SEM) analysis provided a more detailed material of leaves, while an energy dispersive X-Ray (EDX) helped observe elemental composition. Phytochemical content analysis ran by histochemical analysis. The results showed that the telomere lengths of young female, male, and monoecious leaves differed at 381, 391, and 396.66, respectively. According to the SEM analysis, nonsignificant differences occurred in the leaf surface of the three sexes of highland papaya leaves. Observation with EDX showed that sodium (1.98%) surfaced in female plant leaves; however, sodium and chlorine did not show in male plants. Monoecious leaves had sodium and chlorine at 0.88% and 0.28%, respectively. The histochemical analysis provided an overview of the distribution of flavonoids and tannins in young leaves of highland papaya. A discovery also noted the existence of both compounds in the adaxial and abaxial epidermis, mesophyll, xylem, phloem, sheath parenchyma, secretory cavities, and trichomes. The study concluded that sex affects telomere length, elemental composition, and the phytochemical content of highland papaya leaves. The study suggests that knowing the highland papaya’s sex is vital in plant breeding and genetics and could help improve plant health and productivity.
{"title":"SEX EFFECT ON THE TELOMER LENGTH, ELEMENTAL COMPOSITION, AND PHYTOCHEMICAL CONTENT OF HIGHLAND PAPAYA (VASCONCELLEA PUBESCENS A.DC.) LEAVES","authors":"A. N. Laily, B. S. Daryono, A. Purwantoro, Prof. Zahoor Ahmed, Soomro","doi":"10.54910/sabrao2023.55.4.13","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.13","url":null,"abstract":"Highland papaya (Vasconcellea pubescens A.DC.) is a monoecious and dioecious plant with female and male organs. In Indonesia, three sexes of highland papaya exist with different telomere lengths, which protect chromosomes and deoxyribonucleic acid (DNA) from damage. Therefore, the purposeful study aimed to determine the telomere length, elemental composition, and phytochemical content of highland papaya leaves belonging to their different sexes. The telomere length observed and studied in said leaves (female, male, and monoecious) used the polymerase chain reaction (PCR) procedure. A scanning electron microscopy (SEM) analysis provided a more detailed material of leaves, while an energy dispersive X-Ray (EDX) helped observe elemental composition. Phytochemical content analysis ran by histochemical analysis. The results showed that the telomere lengths of young female, male, and monoecious leaves differed at 381, 391, and 396.66, respectively. According to the SEM analysis, nonsignificant differences occurred in the leaf surface of the three sexes of highland papaya leaves. Observation with EDX showed that sodium (1.98%) surfaced in female plant leaves; however, sodium and chlorine did not show in male plants. Monoecious leaves had sodium and chlorine at 0.88% and 0.28%, respectively. The histochemical analysis provided an overview of the distribution of flavonoids and tannins in young leaves of highland papaya. A discovery also noted the existence of both compounds in the adaxial and abaxial epidermis, mesophyll, xylem, phloem, sheath parenchyma, secretory cavities, and trichomes. The study concluded that sex affects telomere length, elemental composition, and the phytochemical content of highland papaya leaves. The study suggests that knowing the highland papaya’s sex is vital in plant breeding and genetics and could help improve plant health and productivity.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44848019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.11
M. A. Al-Shakarchi
Rhizobia are important bacteria, playing a vital role in atmospheric nitrogen fixation. In addition, it increases soil fertility, enhancing crop production and preserving the soil from pollution resulting from inorganic fertilizers’ use. From 81 bacteria samples, nine isolates segregated from nine types of leguminous crops are economically valuable for cultivation within nine different locations in Nineveh Governorate, Iraq. The isolates underwent initial diagnosis by phenotypical and cultural characteristics and host specialization tests. Molecular characterization also ran on the selected bacterial isolates. Performing the 16S rRNA region amplification applied forward primer 27F and a reverse primer 1541R using the polymerase chain reaction (PCR) technique. The results of restriction fragment length polymorphism (RFLP-PCR) employing three endonuclease enzymes showed that EcoRI had similar sequences among the isolates under study but significantly different for MspI and HaeIII. Based on the analysis of results using the statistical program MVSP version 3.22, the degree of similarity divided the isolates into three groups. Results revealed that there were genetic differences between the isolates and the suppressing enzymes, providing more accurate results in recognizing the similarity among the bacterial isolates as a result of the difference in the location and environmental conditions from which the bacteria were isolated, in addition to the different agricultural processes used for those regions.
{"title":"GENETIC DIVERSITY AND PHYLOGENY OF RHIZOBIA ISOLATED FROM NODULES USING RFLP-PCR TECHNIQUE IN NINEVEH PROVINCE, IRAQ","authors":"M. A. Al-Shakarchi","doi":"10.54910/sabrao2023.55.4.11","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.11","url":null,"abstract":"Rhizobia are important bacteria, playing a vital role in atmospheric nitrogen fixation. In addition, it increases soil fertility, enhancing crop production and preserving the soil from pollution resulting from inorganic fertilizers’ use. From 81 bacteria samples, nine isolates segregated from nine types of leguminous crops are economically valuable for cultivation within nine different locations in Nineveh Governorate, Iraq. The isolates underwent initial diagnosis by phenotypical and cultural characteristics and host specialization tests. Molecular characterization also ran on the selected bacterial isolates. Performing the 16S rRNA region amplification applied forward primer 27F and a reverse primer 1541R using the polymerase chain reaction (PCR) technique. The results of restriction fragment length polymorphism (RFLP-PCR) employing three endonuclease enzymes showed that EcoRI had similar sequences among the isolates under study but significantly different for MspI and HaeIII. Based on the analysis of results using the statistical program MVSP version 3.22, the degree of similarity divided the isolates into three groups. Results revealed that there were genetic differences between the isolates and the suppressing enzymes, providing more accurate results in recognizing the similarity among the bacterial isolates as a result of the difference in the location and environmental conditions from which the bacteria were isolated, in addition to the different agricultural processes used for those regions.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42715396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.36
A. H. Hamad, A. H. Abdulmajeed, Z. M. Abdulrazzaq, Prof. Naqib Ullah, Khan
The presented research carried out in the growing season of 2020 at the Department of Horticulture and Landscape, College of Agriculture, University of Anbar, Iraq, investigated the impact of mineral (chemical) and organic (birds) fertilization and foliar application of the humic acid on the active substances of the moringa (Moringa oleifera) plant leaves. The treatments included an organic fertilizer (300 g plant-1 ), mineral fertilizer DAP (225 g plant-1 ), and humic acid (3000 ppm). The experiment ran in a randomized complete block design (RCBD) with three replications. Results revealed that adding organic and mineral fertilizers and foliar application of humic acid affected and increased flavonoids significantly in moringa plant leaves, such as, six flavonoid components, i.e., catechin, quercetin, luteolin, rutin, apigenin, and kaempferol, amounting to 73.83, 21.51, 3.344, 20.55, 7.53, and 2.09 µg L -1 , respectively, compared with the control treatment (2.56, 3.22, 1.304, 2.25, 1.88, and 0.29 ppm, respectively).
本研究于2020年生长季节在伊拉克安巴尔大学农学院园艺与景观系进行，调查了矿物(化学)和有机(鸟类)施肥以及叶面施用腐植酸对辣木(辣木)植物叶片活性物质的影响。处理包括有机肥(300 g plant-1)、矿肥DAP (225 g plant-1)和腐植酸(3000 ppm)。实验采用随机完全区组设计(RCBD)，重复3次。结果表明，与对照处理(分别为2.56、3.22、1.304、2.25、1.88和0.29 ppm)相比，添加有机肥和矿物肥以及叶面施用腐植酸对辣木叶片中黄酮类成分儿茶素、槲皮素、木犀草素、芦丁、芹菜素和山樟酚6种黄酮类成分的含量分别为73.83、21.51、3.344、20.55、7.53和2.09µg L -1，并显著提高了辣木叶片中黄酮类成分的含量。
{"title":"EFFECT OF ORGANIC AND MINERAL FERTILIZATION AND HUMIC ACID APPLICATION ON INCREASING THE FLAVONOID CONTENT IN MORINGA OLEIFERA LEAVES","authors":"A. H. Hamad, A. H. Abdulmajeed, Z. M. Abdulrazzaq, Prof. Naqib Ullah, Khan","doi":"10.54910/sabrao2023.55.4.36","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.36","url":null,"abstract":"The presented research carried out in the growing season of 2020 at the Department of Horticulture and Landscape, College of Agriculture, University of Anbar, Iraq, investigated the impact of mineral (chemical) and organic (birds) fertilization and foliar application of the humic acid on the active substances of the moringa (Moringa oleifera) plant leaves. The treatments included an organic fertilizer (300 g plant-1 ), mineral fertilizer DAP (225 g plant-1 ), and humic acid (3000 ppm). The experiment ran in a randomized complete block design (RCBD) with three replications. Results revealed that adding organic and mineral fertilizers and foliar application of humic acid affected and increased flavonoids significantly in moringa plant leaves, such as, six flavonoid components, i.e., catechin, quercetin, luteolin, rutin, apigenin, and kaempferol, amounting to 73.83, 21.51, 3.344, 20.55, 7.53, and 2.09 µg L -1 , respectively, compared with the control treatment (2.56, 3.22, 1.304, 2.25, 1.88, and 0.29 ppm, respectively).","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47402593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.17
T. Abbas
Faisalabad industrial units discharge effluents and associated toxic chemicals into the environment, deteriorating ecological conditions and ecosystem health. Morphoanatomical changes in some medicinally important native species (Calotropis procera, Eclipta alba, Phyla nodiflora, and Ranunculus sceleratus) exposed to heavy industrial pollution gained evaluation. These species of choice were due to their widespread distribution in the area. Ten sites selected in the River Chenab, Chiniot, had three near point source pollution of Faisalabad industries within the 500-m radius (polluted) at three drains and two sites inside the river after each drain point source with the control site at 14 km after from the first industrial drain point source. The general response of all plants to effluents was growth retardation. Plant height increased significantly in all species from river sites. An increase in tallness was more prominent in species like C. procera and P. nodiflora. An increase in stem sclerification in C. procera and E. alba from polluted sites occurred, which indicated a better ability to tolerate industrial pollution. Remarkable increases in stem and leaf epidermis, intensive stem sclerenchyma, and closely packed stem vascular bundles in C. procera appeared, which could increase resistance to industrial pollution. Most of the morphoanatomical parameters notably attained a decrease in E. alba, the most vulnerable species. Plant survival depends on particular structural changes in dermal, mechanical, parenchymatous, and vascular tissues. Overall, industrial pollution adversely impacts plant morphological and micromorphological features, although the reaction of specific species to industrial contamination varies. The study determined that stem and leaf anatomical features, such as, epidermis size and storage tissue thickness, are suitable morphoanatomical markers for industrial pollution biomonitoring. Internal modifications of plants vegetating different industrial contaminated sites played a significant role in high tolerance levels.
{"title":"MICROMORPHOLOGICAL AND ANATOMICAL RESPONSES OF NATIVE DICOTS TO INDUSTRIAL EFFLUENTS RELEASED FROM CONTAMINATED REGION OF THE CHENAB RIVER IN PAKISTAN","authors":"T. Abbas","doi":"10.54910/sabrao2023.55.4.17","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.17","url":null,"abstract":"Faisalabad industrial units discharge effluents and associated toxic chemicals into the environment, deteriorating ecological conditions and ecosystem health. Morphoanatomical changes in some medicinally important native species (Calotropis procera, Eclipta alba, Phyla nodiflora, and Ranunculus sceleratus) exposed to heavy industrial pollution gained evaluation. These species of choice were due to their widespread distribution in the area. Ten sites selected in the River Chenab, Chiniot, had three near point source pollution of Faisalabad industries within the 500-m radius (polluted) at three drains and two sites inside the river after each drain point source with the control site at 14 km after from the first industrial drain point source. The general response of all plants to effluents was growth retardation. Plant height increased significantly in all species from river sites. An increase in tallness was more prominent in species like C. procera and P. nodiflora. An increase in stem sclerification in C. procera and E. alba from polluted sites occurred, which indicated a better ability to tolerate industrial pollution. Remarkable increases in stem and leaf epidermis, intensive stem sclerenchyma, and closely packed stem vascular bundles in C. procera appeared, which could increase resistance to industrial pollution. Most of the morphoanatomical parameters notably attained a decrease in E. alba, the most vulnerable species. Plant survival depends on particular structural changes in dermal, mechanical, parenchymatous, and vascular tissues. Overall, industrial pollution adversely impacts plant morphological and micromorphological features, although the reaction of specific species to industrial contamination varies. The study determined that stem and leaf anatomical features, such as, epidermis size and storage tissue thickness, are suitable morphoanatomical markers for industrial pollution biomonitoring. Internal modifications of plants vegetating different industrial contaminated sites played a significant role in high tolerance levels.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45149314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.32
S. S. Alaarage, A. Alamery, Dr. Osama Osman Atallah
A field experiment done in the spring of 2022 on Sorghum bicolor L. cultivars with different nitrogen and biofertilizer fertilizer regimes proceeded at the Ibn-Al-Bitar Vocational Preparatory School, District Al-Hussainiya, Holy Governorate of Kerbala. Sowing in a randomized complete block design (RCBD) with a factorial experiment and three replications took place in the third week of March 2022, and harvesting in the third week of July 2022. The trial included two factors; the first had three sorghum cultivars (V1 = Al-Khair, V2 = Rabeh, and V3 = Bohuth 70). The second factor was combinations of nitrogen and biofertilizers, i.e., F0 = the complete dose of nitrogen fertilizer (320 kg N ha-1 ); F1 = 3/4 nitrogen fertilizer + Azotobacter; F2 = 1/2 nitrogen fertilizer + Azotobacter; F3 = 1/4 nitrogen fertilizer + Azotobacter, and F4 = Azotobacter with no nitrogen fertilizer. The results showed the superiority of cultivar Bohuth 70 for the traits yield per plant (116.2 g plant-1 ) and nitrogen, phosphorus, and protein percentages in grains (2.212%, 0.3212%, and 13.82%, respectively). Cultivar Al-Khair excelled in total chlorophyll, ash, and potassium percentages in grains (1.057 mg g-1 , 2.165%, and 2.085%, respectively). As for the interaction, the combined treatments V3F0 excelled in the traits of yield per plant (134.4 g plant-1 ), nitrogen (2.610%), and protein percentages (16.31%), while the interaction of V1F3 performed better for the variables ash and potassium percentages (2.600% and 2.160%, respectively). However, the reaction of V3F1 surpassed phosphorus and potassium percentages (0.3615% and 2.160%). The research happened to know the extent to reducing the quantities of recommended chemical nitrogen fertilizers and their effect on the qualitative.
2022年春天，在克尔巴拉圣省Al Hussainiya区的Ibn Al Bitar职业预备学校，对具有不同氮和生物肥料制度的高粱双色品种进行了田间试验。在2022年3月的第三周，采用随机完全区组设计（RCBD）进行了析因实验和三次重复播种，并在2022年7月的第四周收获。试验包括两个因素；第一个有三个高粱品种（V1=Al Khair，V2=Rabeh，V3=Bohuth 70）。第二个因素是氮和生物肥料的组合，即F0=氮肥的完整剂量（320 kg N ha-1）；F1=3/4氮肥+固氮菌；F2=1/2氮肥+固氮菌；F3=1/4氮肥+固氮菌，F4=无氮肥的固氮菌。结果表明，品种Bohuth 70在单株产量（116.2g株-1）和籽粒氮、磷、蛋白质含量（分别为2.212%、0.3212%和13.82%）方面具有优势。品种Al Khair在籽粒中的总叶绿素、灰分和钾百分比方面表现优异（分别为1.057 mg g-1、2.165%和2.085%）。就交互作用而言，组合处理V3F0在单株产量（134.4g plant-1）、氮（2.610%）和蛋白质百分比（16.31%）方面表现优异，而V1F3在灰分和钾百分比方面表现更好（分别为2.600%和2.160%）。但V3F1的反应超过了磷和钾的比例（0.3615%和2.160%）。
{"title":"NITROGEN AND BIOFERTILIZATION EFFECTS ON BIOCHEMICAL, QUALITATIVE, AND PHYSIOLOGICAL TRAITS OF SORGHUM (SORGHUM BICOLOR L.)","authors":"S. S. Alaarage, A. Alamery, Dr. Osama Osman Atallah","doi":"10.54910/sabrao2023.55.4.32","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.32","url":null,"abstract":"A field experiment done in the spring of 2022 on Sorghum bicolor L. cultivars with different nitrogen and biofertilizer fertilizer regimes proceeded at the Ibn-Al-Bitar Vocational Preparatory School, District Al-Hussainiya, Holy Governorate of Kerbala. Sowing in a randomized complete block design (RCBD) with a factorial experiment and three replications took place in the third week of March 2022, and harvesting in the third week of July 2022. The trial included two factors; the first had three sorghum cultivars (V1 = Al-Khair, V2 = Rabeh, and V3 = Bohuth 70). The second factor was combinations of nitrogen and biofertilizers, i.e., F0 = the complete dose of nitrogen fertilizer (320 kg N ha-1 ); F1 = 3/4 nitrogen fertilizer + Azotobacter; F2 = 1/2 nitrogen fertilizer + Azotobacter; F3 = 1/4 nitrogen fertilizer + Azotobacter, and F4 = Azotobacter with no nitrogen fertilizer. The results showed the superiority of cultivar Bohuth 70 for the traits yield per plant (116.2 g plant-1 ) and nitrogen, phosphorus, and protein percentages in grains (2.212%, 0.3212%, and 13.82%, respectively). Cultivar Al-Khair excelled in total chlorophyll, ash, and potassium percentages in grains (1.057 mg g-1 , 2.165%, and 2.085%, respectively). As for the interaction, the combined treatments V3F0 excelled in the traits of yield per plant (134.4 g plant-1 ), nitrogen (2.610%), and protein percentages (16.31%), while the interaction of V1F3 performed better for the variables ash and potassium percentages (2.600% and 2.160%, respectively). However, the reaction of V3F1 surpassed phosphorus and potassium percentages (0.3615% and 2.160%). The research happened to know the extent to reducing the quantities of recommended chemical nitrogen fertilizers and their effect on the qualitative.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42143613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.15
D. Juraev, O. Amanov, Sh. Dilmurodov, N. Boysunov, S. Turaeva, N. Mamadjanova, D. Raimova
The presented study aims to select the early-maturing and high-yielding winter wheat genotypes via the assessment of various eco-geographical groups and to illustrate their scientific significance for the diverse environmental conditions of the Kashkadarya Region of the Republic of Uzbekistan. Twentyfour advanced wheat lines underwent selection and evaluation for comparison with six regional and promising cultivars to further improve and use for crossbreeding. Overall, eight cultivars showed promising in the desert zone of the Kashkadarya Region, 24 in the middle area, and 13 in the region’s foothills, while selecting five for the desert zone, six in the middle, and 10 in the foothills of Uzbekistan. Seventeen cultivars and advanced lines attained selection for the desert area of District Kasbi with higher protein content (14%), 21 in the middle zone of District Karshi, and 22 in the foothills of District Shahrizabz. Cultivars and advanced lines selected for the desert area of District Kasbi, in the middle zone of District Karshi, and in the foothills of District Shahrizabz numbered 14, 14, and three, respectively, where the gluten content was more than 30%. Weather conditions also affect the wheat genotype yields in the irrigated areas, and due to less rainfall in March-May of 2011, in the desert and foothills, the average yield declined by 0.2–0.23 and 0.15–0.25 t/ha in the deserts and foothills, respectively, compared with other years. The precipitation in March-May strongly correlated (r = 0.47) with the grain yield in winter bread wheat.
{"title":"WINTER WHEAT ASSESSMENT FOR GROWTH, GRAIN YIELD, AND QUALITY PARAMETERS UNDER DIVERSE SOIL AND CLIMATIC CONDITIONS","authors":"D. Juraev, O. Amanov, Sh. Dilmurodov, N. Boysunov, S. Turaeva, N. Mamadjanova, D. Raimova","doi":"10.54910/sabrao2023.55.4.15","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.15","url":null,"abstract":"The presented study aims to select the early-maturing and high-yielding winter wheat genotypes via the assessment of various eco-geographical groups and to illustrate their scientific significance for the diverse environmental conditions of the Kashkadarya Region of the Republic of Uzbekistan. Twentyfour advanced wheat lines underwent selection and evaluation for comparison with six regional and promising cultivars to further improve and use for crossbreeding. Overall, eight cultivars showed promising in the desert zone of the Kashkadarya Region, 24 in the middle area, and 13 in the region’s foothills, while selecting five for the desert zone, six in the middle, and 10 in the foothills of Uzbekistan. Seventeen cultivars and advanced lines attained selection for the desert area of District Kasbi with higher protein content (14%), 21 in the middle zone of District Karshi, and 22 in the foothills of District Shahrizabz. Cultivars and advanced lines selected for the desert area of District Kasbi, in the middle zone of District Karshi, and in the foothills of District Shahrizabz numbered 14, 14, and three, respectively, where the gluten content was more than 30%. Weather conditions also affect the wheat genotype yields in the irrigated areas, and due to less rainfall in March-May of 2011, in the desert and foothills, the average yield declined by 0.2–0.23 and 0.15–0.25 t/ha in the deserts and foothills, respectively, compared with other years. The precipitation in March-May strongly correlated (r = 0.47) with the grain yield in winter bread wheat.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41775835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.54910/sabrao2023.55.4.1
S. Alsharari, A. Ibrahim, S. Okasha
Creating a half-diallel cross succeeded among seven diverse canola genotypes. The obtained 21 F1 hybrids with their seven parents underwent three salinity stress levels exposure—3.91 dsm1 (Normal), 6.24 dsm-1 (S1), and 7.81 dsm-1 (S2) —during the 2020/2021 growing seasons. Salinity treatments significantly reduced days to 50% flowering, plant height, number of primary branches, pods/plant, 1000-seed weight, seed yield/plant, seed oil content, relative water content, calcium, potassium, and the ratio between K+ and Na+ compared with a normal condition. Proline content, osmotic pressure, and Na+ were considerably higher under salinity stress conditions. Highly significant differences showed among the parents and hybrids for all traits across the tested environments. General (GCA) and specific (SCA) combining ability effects were highly significant for all attributes. The parental genotypes Serw4 and Pactol resulted as good general combiners for increased seed oil content (SOC), seed yield/plant (SYPP), and some of its components in research environments. The hybrid combinations H2/S × Serw4 and Serw4 × Serw6 were good specific combiners for days to first flower (DTF), number of primary branches (NPB), number of pods per plant (NP), a thousand seed weight (TSW), seed yield per plant (SYPP), seed oil content (SOC), proline content (ProC), Ca++, and K+/Na+. The SDS-PAGE analysis of seed proteins indicated high levels of genetic variability and revealed some vital biochemical markers for salt tolerance.
{"title":"COMBINING ABILITY FOR YIELD, OIL CONTENT, AND PHYSIO-BIOCHEMICAL CHARACTERS OF CANOLA (BRASSICA NAPUS L.) UNDER SALT STRESS CONDITIONS","authors":"S. Alsharari, A. Ibrahim, S. Okasha","doi":"10.54910/sabrao2023.55.4.1","DOIUrl":"https://doi.org/10.54910/sabrao2023.55.4.1","url":null,"abstract":"Creating a half-diallel cross succeeded among seven diverse canola genotypes. The obtained 21 F1 hybrids with their seven parents underwent three salinity stress levels exposure—3.91 dsm1 (Normal), 6.24 dsm-1 (S1), and 7.81 dsm-1 (S2) —during the 2020/2021 growing seasons. Salinity treatments significantly reduced days to 50% flowering, plant height, number of primary branches, pods/plant, 1000-seed weight, seed yield/plant, seed oil content, relative water content, calcium, potassium, and the ratio between K+ and Na+ compared with a normal condition. Proline content, osmotic pressure, and Na+ were considerably higher under salinity stress conditions. Highly significant differences showed among the parents and hybrids for all traits across the tested environments. General (GCA) and specific (SCA) combining ability effects were highly significant for all attributes. The parental genotypes Serw4 and Pactol resulted as good general combiners for increased seed oil content (SOC), seed yield/plant (SYPP), and some of its components in research environments. The hybrid combinations H2/S × Serw4 and Serw4 × Serw6 were good specific combiners for days to first flower (DTF), number of primary branches (NPB), number of pods per plant (NP), a thousand seed weight (TSW), seed yield per plant (SYPP), seed oil content (SOC), proline content (ProC), Ca++, and K+/Na+. The SDS-PAGE analysis of seed proteins indicated high levels of genetic variability and revealed some vital biochemical markers for salt tolerance.","PeriodicalId":21328,"journal":{"name":"Sabrao Journal of Breeding and Genetics","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46516696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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