Pub Date : 2022-02-28DOI: 10.17268/sci.agropecu.2022.006
Gabriela Saravia-Castillo, Lourdes Tapia y Figueroa, Ricardo Borjas-Ventura
Orchids have a long period of production and need to be under the right conditions. Therefore, in vitro propagation is an alternative to reduce production time. This study aimed to determine the optimal in vitro propagation conditions in two species of orchids, Cattleya maxima Lindl. and Phalaenopsis amabilis (L.) Blume. For this goal, two experiments were carried out: multiplication and rooting, for both species. In the multiplication experiment, the treatments were differentiated according to the complement of MS (Murashige and Skoog medium), banana flour, kinetin or 6-Benzylaminopurine (BAP). Meanwhile, in the rooting experiment, the complements were banana flour, Indol-3-butiric acid (IBA), Naphthaleneacetic acid (NAA) or 2,4-Dichlorophenoxyacetic acid (2,4-D). According to the results, banana flour (50 g.L-1) increased the plantlet height in C. maxima (multiplication) and P. amabilis (rooting). Kinetin (0.003 g.L-1) and BAP (0.005 g.L-1) increased the number of shoots and leaves in C. maxima, in multiplication experiment. In rooting experiment, NAA (0.003 g.L-1) increased significantly the number of leaves in C. maxima. The root formation was notably stimulated by banana flour in both species. Likewise, 2,4-D (0.003 g.L-1) can cause phytotoxic effects and inhibit root formation. In conclusion, C. maxima was more robust than P. amabilis since in all studied variables the former presented the highest values. Furthermore, C. maxima responds adequately to application of kinetin and BAP to increase the aerial part of the plant, however, they inhibit the root formation. In contrast, kinetin and BAP do not limit root growth in P. amabilis.
兰花的生产周期很长,需要在合适的条件下进行。因此,体外繁殖是减少生产时间的一种替代方法。本研究旨在确定两种兰花Cattleya maxima Lindl的最佳体外繁殖条件。和Phalaenopsis amabilis(L.)Blume。为此,进行了两个实验:两个物种的繁殖和生根。在增殖实验中,根据MS(Murashige和Skoog培养基)、香蕉粉、激动素或6-苄基氨基嘌呤(BAP)的互补性来分化处理。同时,在生根试验中,补充物为香蕉粉、吲哚-3-丁酸(IBA)、萘乙酸(NAA)或2,4-二氯苯氧乙酸(2,4-D)。结果表明,香蕉粉(50g.L-1)可提高大花(繁殖)和紫茉莉(生根)的植株高度。在增殖实验中,Kinetin(0.003 g.L-1)和BAP(0.005g.L-1)增加了C.maxima的芽和叶的数量。在生根试验中,NAA(0.003g.L-1)显著增加了C.maxima的叶片数量。香蕉粉对两种植物根系的形成都有显著的刺激作用。同样,2,4-D(0.003 g.L-1)可引起植物毒性作用并抑制根的形成。总之,C.maximas比P.amabilis更稳健,因为在所有研究的变量中,前者的值最高。此外,C.maxima对激动素和BAP的施用有充分的反应,以增加植物的地上部分,然而,它们抑制根的形成。相反,激动素和BAP不限制紫茉莉的根系生长。
{"title":"Auxins and Cytokinins elicit a differentiated response in the formation of shoots and roots in Cattleya maxima Lindl and Phalaenopsis amabilis (L) Blume","authors":"Gabriela Saravia-Castillo, Lourdes Tapia y Figueroa, Ricardo Borjas-Ventura","doi":"10.17268/sci.agropecu.2022.006","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.006","url":null,"abstract":"Orchids have a long period of production and need to be under the right conditions. Therefore, in vitro propagation is an alternative to reduce production time. This study aimed to determine the optimal in vitro propagation conditions in two species of orchids, Cattleya maxima Lindl. and Phalaenopsis amabilis (L.) Blume. For this goal, two experiments were carried out: multiplication and rooting, for both species. In the multiplication experiment, the treatments were differentiated according to the complement of MS (Murashige and Skoog medium), banana flour, kinetin or 6-Benzylaminopurine (BAP). Meanwhile, in the rooting experiment, the complements were banana flour, Indol-3-butiric acid (IBA), Naphthaleneacetic acid (NAA) or 2,4-Dichlorophenoxyacetic acid (2,4-D). According to the results, banana flour (50 g.L-1) increased the plantlet height in C. maxima (multiplication) and P. amabilis (rooting). Kinetin (0.003 g.L-1) and BAP (0.005 g.L-1) increased the number of shoots and leaves in C. maxima, in multiplication experiment. In rooting experiment, NAA (0.003 g.L-1) increased significantly the number of leaves in C. maxima. The root formation was notably stimulated by banana flour in both species. Likewise, 2,4-D (0.003 g.L-1) can cause phytotoxic effects and inhibit root formation. In conclusion, C. maxima was more robust than P. amabilis since in all studied variables the former presented the highest values. Furthermore, C. maxima responds adequately to application of kinetin and BAP to increase the aerial part of the plant, however, they inhibit the root formation. In contrast, kinetin and BAP do not limit root growth in P. amabilis.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44012907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-12DOI: 10.17268/sci.agropecu.2022.001
P. Şanlıbaba, S. Buzrul
Biocontrol applications such as using phages against the contamination of Listeria monocytogenes are promising trends in terms of reducing the use of chemical additives in food industry. The aim of this study was to determine the effectiveness of Listex P100 phage (phage P100) on different Listeria monocytogenes strains (PL2, PL3, PL9 and PL10) in pasteurized milk and broth. Survival data of L. monocytogenes were successfully described by Weibull model. Time parameter of the Weibull model was used to evaluate the phage-resistances of L. monocytogenes strains. The reduction of L. monocytogenes was greater in broth than in milk regardless of the temperature level and it was significantly higher at 30 °C than at 4 °C in both media. The reductions of L. monocytogenes strains by the phage treatment were between 2.7 to 3.4 log10 units at 30 °C and 1.4 to 2.1 log10 units at 4 °C after 4 days of incubation in broth whereas 1.9 to 2.9 log10 units and 1.0 to1.6 log10 units were observed after 4 days of incubation in milk at 30 °C and 4 °C, respectively. It was found that L. monocytogenes PL2 is the most phage-resistant strain in broth at 30 °C and at 4 °C, and in milk at 30 °C, while L. monocytogenes PL9 is the most phage-resistant L. monocytogenes strain in milk at 4 °C. This study demonstrated P100 phage could be used to control L. monocytogenes counts in milk.
{"title":"Control of Listeria monocytogenes in milk by using phage cocktail","authors":"P. Şanlıbaba, S. Buzrul","doi":"10.17268/sci.agropecu.2022.001","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.001","url":null,"abstract":"Biocontrol applications such as using phages against the contamination of Listeria monocytogenes are promising trends in terms of reducing the use of chemical additives in food industry. The aim of this study was to determine the effectiveness of Listex P100 phage (phage P100) on different Listeria monocytogenes strains (PL2, PL3, PL9 and PL10) in pasteurized milk and broth. Survival data of L. monocytogenes were successfully described by Weibull model. Time parameter of the Weibull model was used to evaluate the phage-resistances of L. monocytogenes strains. The reduction of L. monocytogenes was greater in broth than in milk regardless of the temperature level and it was significantly higher at 30 °C than at 4 °C in both media. The reductions of L. monocytogenes strains by the phage treatment were between 2.7 to 3.4 log10 units at 30 °C and 1.4 to 2.1 log10 units at 4 °C after 4 days of incubation in broth whereas 1.9 to 2.9 log10 units and 1.0 to1.6 log10 units were observed after 4 days of incubation in milk at 30 °C and 4 °C, respectively. It was found that L. monocytogenes PL2 is the most phage-resistant strain in broth at 30 °C and at 4 °C, and in milk at 30 °C, while L. monocytogenes PL9 is the most phage-resistant L. monocytogenes strain in milk at 4 °C. This study demonstrated P100 phage could be used to control L. monocytogenes counts in milk.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44802476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viruses have been present throughout human history, causing diseases due to infections and food poisoning; they have caused frequent public health problems worldwide. These illnesses are usually mild, moderate, or severe in nature. The personal hygiene of food handlers and processing processes should be checked periodically. Virus detection protocols and safety measures should be continually reviewed as viruses change their mode of infection. The objective of this review was to discuss the possible routes of virus transmission to humans through food. Important topics have been reviewed such as: definition of food viruses, presence, and types of viruses in food, enteric viruses, zoonotic viruses, water as a means of transmission, risks of infection, other non-conventional foods as potential transmitters of viruses and food safety, in addition to current and future challenges, research work on viruses more resistant to heat treatments in food should be sought. Also, future work on survival time of active viruses on food surfaces. In addition, studies that determine the mechanisms of virus mutation in relation to the conditions of food handling and processing.
{"title":"Food as a transmitter of viruses: A review","authors":"Teofilo Espinoza Tellez, Roberto Quevedo - León, Yennifer Ávila - Pizarro","doi":"10.17268/sci.agropecu.2022.003","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.003","url":null,"abstract":"Viruses have been present throughout human history, causing diseases due to infections and food poisoning; they have caused frequent public health problems worldwide. These illnesses are usually mild, moderate, or severe in nature. The personal hygiene of food handlers and processing processes should be checked periodically. Virus detection protocols and safety measures should be continually reviewed as viruses change their mode of infection. The objective of this review was to discuss the possible routes of virus transmission to humans through food. Important topics have been reviewed such as: definition of food viruses, presence, and types of viruses in food, enteric viruses, zoonotic viruses, water as a means of transmission, risks of infection, other non-conventional foods as potential transmitters of viruses and food safety, in addition to current and future challenges, research work on viruses more resistant to heat treatments in food should be sought. Also, future work on survival time of active viruses on food surfaces. In addition, studies that determine the mechanisms of virus mutation in relation to the conditions of food handling and processing.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49035321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-12DOI: 10.17268/sci.agropecu.2022.002
Werther Kern Falcón, Marco Schwartz Melgar, Ricardo Marchant Silva
The circular economy, as a factor of sustainable development, is an innovative practice of resource allocation that considers the production of goods and the reuse of their waste. Society values the circular economy, since it contributes to the reduction of negative externalities arising from the operation of certain industries that generate waste that is harmful to the environment. In this work, the magnitude of the negative externality represented by the accumulation of the waste called "alperujo" in the olive sector in Chile is evaluated, identifying, and validating new businesses developed from its reuse. For this purpose, a portfolio of viable options for the valorization of alperujo was constituted, based on a contextual analysis, the application of Product-Market Fit and the participation of a focus group, which allowed estimating the reduction of the negative externality, because of the use of alperujo. This industry externality comes from the generation of 120,000 t/year of waste, with an estimated value of USD 10.48 million. The alperujo, in the form of paste or extract, can be incorporated in the production of snacks, antioxidant drinks and yogurt. The sum of the NPV of the three businesses reaches USD 11.63 million, which allows compensating the estimated negative externality with a favorable margin, using 1.8% of the alperujo generated annually in Chile. This study contributes to the validation of the circular business model in a relevant sector of the Chilean export economy.
{"title":"Reduction of negative externalities due to the use of waste in product development: circular economy in the Chilean olive industry","authors":"Werther Kern Falcón, Marco Schwartz Melgar, Ricardo Marchant Silva","doi":"10.17268/sci.agropecu.2022.002","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.002","url":null,"abstract":"The circular economy, as a factor of sustainable development, is an innovative practice of resource allocation that considers the production of goods and the reuse of their waste. Society values the circular economy, since it contributes to the reduction of negative externalities arising from the operation of certain industries that generate waste that is harmful to the environment. In this work, the magnitude of the negative externality represented by the accumulation of the waste called \"alperujo\" in the olive sector in Chile is evaluated, identifying, and validating new businesses developed from its reuse. For this purpose, a portfolio of viable options for the valorization of alperujo was constituted, based on a contextual analysis, the application of Product-Market Fit and the participation of a focus group, which allowed estimating the reduction of the negative externality, because of the use of alperujo. This industry externality comes from the generation of 120,000 t/year of waste, with an estimated value of USD 10.48 million. The alperujo, in the form of paste or extract, can be incorporated in the production of snacks, antioxidant drinks and yogurt. The sum of the NPV of the three businesses reaches USD 11.63 million, which allows compensating the estimated negative externality with a favorable margin, using 1.8% of the alperujo generated annually in Chile. This study contributes to the validation of the circular business model in a relevant sector of the Chilean export economy.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41441814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-15DOI: 10.17268/sci.agropecu.2021.057
Aybel Almanza Cano, Hanna Cáceres Yparraguirre, María Del Rocío Torres, David Saravia Navarro, Raúl Blas Sevillano
Pisco is a distillate of cultural and economic importance for Peru, made from heterogeneous vine varieties called "pisqueras", covered by a Pisco Denomination of Origin legislation. The aim was to confirm the varietal identity of eight accessions conserved in the Germplasm Collection of the Centre for Productive Innovation and Technology Transfer Agroindustrial Ica (CITEagroindustrial), using 9 standardized microsatellite loci (SSR) and 14 morphological descriptors according to the International Organisation of Vine and Wine (OIV) to identify grapevine varieties. The SSR molecular profiles of 7 accessions were identified as varieties registered in the Vitis International Variety Catalog (VIVC) and only one had no association. Of these, Mollar, Albilla, Italia, Quebranta and Uvina accessions, were associated with Mollar Cano (VIVC 7901), Palomino Fino (VIVC 8888), Muscat of Alexandria (VIVC 8241), Quebranta (VIVC 9840) and Jacquez (VIVC 5627), respectively. The SSR profile of the Torontel accession was not associated with any variety registered in the VIVC and the Negra Criolla and Moscatel accessions were associated with the Spanish variety Listan Prieto (VIVC 6860). According to the ampelographic observations, it was determined that Moscatel presented phenotypic variations with respect to Negra Criolla, although at the molecular level it was perfectly associated with Listan Prieto, indicating that it would be a clonal variation. In addition, 7 variables were discriminant to identify the accessions evaluated. The molecular and ampelographic analysis allowed to clarify the varietal identity of the accessions conserved at CITEagroindustrial, Ica, which plays a preponderant role as a reference regarding the identification of the varieties used for the elaboration of Pisco in Peru.
{"title":"Molecular and ampelographic characterizationof Pisco grapevine accessions conserved in a germplasm collection center of the Ica, Peru","authors":"Aybel Almanza Cano, Hanna Cáceres Yparraguirre, María Del Rocío Torres, David Saravia Navarro, Raúl Blas Sevillano","doi":"10.17268/sci.agropecu.2021.057","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2021.057","url":null,"abstract":"Pisco is a distillate of cultural and economic importance for Peru, made from heterogeneous vine varieties called \"pisqueras\", covered by a Pisco Denomination of Origin legislation. The aim was to confirm the varietal identity of eight accessions conserved in the Germplasm Collection of the Centre for Productive Innovation and Technology Transfer Agroindustrial Ica (CITEagroindustrial), using 9 standardized microsatellite loci (SSR) and 14 morphological descriptors according to the International Organisation of Vine and Wine (OIV) to identify grapevine varieties. The SSR molecular profiles of 7 accessions were identified as varieties registered in the Vitis International Variety Catalog (VIVC) and only one had no association. Of these, Mollar, Albilla, Italia, Quebranta and Uvina accessions, were associated with Mollar Cano (VIVC 7901), Palomino Fino (VIVC 8888), Muscat of Alexandria (VIVC 8241), Quebranta (VIVC 9840) and Jacquez (VIVC 5627), respectively. The SSR profile of the Torontel accession was not associated with any variety registered in the VIVC and the Negra Criolla and Moscatel accessions were associated with the Spanish variety Listan Prieto (VIVC 6860). According to the ampelographic observations, it was determined that Moscatel presented phenotypic variations with respect to Negra Criolla, although at the molecular level it was perfectly associated with Listan Prieto, indicating that it would be a clonal variation. In addition, 7 variables were discriminant to identify the accessions evaluated. The molecular and ampelographic analysis allowed to clarify the varietal identity of the accessions conserved at CITEagroindustrial, Ica, which plays a preponderant role as a reference regarding the identification of the varieties used for the elaboration of Pisco in Peru.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47584842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-15DOI: 10.17268/sci.agropecu.2021.063
Kevin P. Ccoyllar-Quintanilla, Ana F. Zaravia-Povis, Arlitt A. Lozano-Povis, José V. Cornejo-Tueros
Quinoa ( Chenopodium quinoa Willd) is an agricultural crop that was domesticated in the Andes of Peru and Bolivia, and that has managed to be produced in any type of soil, characterized by its high amount of nutrients and because it is a species that comes to combat abiotic stresses such as drought, salinity, among others. For example, in the face of drought and high temperatures, this crop becomes very tolerant as it has shown that it uses smaller amounts of water and adapts to fluctuations in the climate during biomass production, which makes it a great alternative for regions, arid and semi-arid. In this sense, the objective of this research article was to determine the vulnerability of the cultivation of quinoa to environmental stress events in the high Andean regions of Peru, through the development and analysis of the current distribution model and its future projections for 2050 and 2070 in scenarios RCP 2.6, RCP 4.5 and RCP 8.5 considering their points of presence and the different bioclimates. Likewise, determine by means of the BAM diagram the optimal zones for its development, considering the abiotic, biotic and accessibility variables. In conclusion, it was obtained that quinoa becomes very tolerant to abiotic stress events such as drought and high temperatures, highlighting as the most optimal areas for its development the departments of Puno, Apurímac, Ayacucho and Cuzco .
{"title":"Vulnerability of quinoa (Chenopodium quinoaWilld) to environmental stress events in the high Andean regions of Peru","authors":"Kevin P. Ccoyllar-Quintanilla, Ana F. Zaravia-Povis, Arlitt A. Lozano-Povis, José V. Cornejo-Tueros","doi":"10.17268/sci.agropecu.2021.063","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2021.063","url":null,"abstract":"Quinoa ( Chenopodium quinoa Willd) is an agricultural crop that was domesticated in the Andes of Peru and Bolivia, and that has managed to be produced in any type of soil, characterized by its high amount of nutrients and because it is a species that comes to combat abiotic stresses such as drought, salinity, among others. For example, in the face of drought and high temperatures, this crop becomes very tolerant as it has shown that it uses smaller amounts of water and adapts to fluctuations in the climate during biomass production, which makes it a great alternative for regions, arid and semi-arid. In this sense, the objective of this research article was to determine the vulnerability of the cultivation of quinoa to environmental stress events in the high Andean regions of Peru, through the development and analysis of the current distribution model and its future projections for 2050 and 2070 in scenarios RCP 2.6, RCP 4.5 and RCP 8.5 considering their points of presence and the different bioclimates. Likewise, determine by means of the BAM diagram the optimal zones for its development, considering the abiotic, biotic and accessibility variables. In conclusion, it was obtained that quinoa becomes very tolerant to abiotic stress events such as drought and high temperatures, highlighting as the most optimal areas for its development the departments of Puno, Apurímac, Ayacucho and Cuzco .","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47024128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-15DOI: 10.17268/sci.agropecu.2021.067
M. Florez-Jalixto, D. Roldán-Acero, J. R. Omote-Sibina, A. Molleda-Ordoñez
{"title":"Biofertilizers and biostimulantsfor agricultural and aquaculture use: Bioprocesses applied to organic by-products of the fishing industry","authors":"M. Florez-Jalixto, D. Roldán-Acero, J. R. Omote-Sibina, A. Molleda-Ordoñez","doi":"10.17268/sci.agropecu.2021.067","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2021.067","url":null,"abstract":"","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43320566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-15DOI: 10.17268/sci.agropecu.2021.064
Javier Andrés Auhing Arcos, Ángel Virgilio Cedeño Moreira, Silvia Saucedo Aguiar, Luis Fernando Vera Benites, Cristhian John Macías Holguín, Hayron Fabricio Canchignia Martínez
{"title":"Biodiversity of ecotypes and aggressivenss ranges of Moniliophthora perniciosa, in Theobroma cacaoL. national of the Ecuadorian Coast","authors":"Javier Andrés Auhing Arcos, Ángel Virgilio Cedeño Moreira, Silvia Saucedo Aguiar, Luis Fernando Vera Benites, Cristhian John Macías Holguín, Hayron Fabricio Canchignia Martínez","doi":"10.17268/sci.agropecu.2021.064","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2021.064","url":null,"abstract":"","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41802878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-15DOI: 10.17268/sci.agropecu.2021.068
Anthony A. Moreira-Morrillo, Ángel V. Cedeño-Moreira, Fabricio Canchignia-Martínez, F. R. Garcés-Fiallos
{"title":"Lasiodiplodiatheobromae(Pat.) Griffon & Maubl [(syn.) Botryodiplodia theobromae Pat] in the cocoa crop: symptoms, biological cycle,and strategies management","authors":"Anthony A. Moreira-Morrillo, Ángel V. Cedeño-Moreira, Fabricio Canchignia-Martínez, F. R. Garcés-Fiallos","doi":"10.17268/sci.agropecu.2021.068","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2021.068","url":null,"abstract":"","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43833951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-15DOI: 10.17268/sci.agropecu.2021.061
R. Aguilar-Anccota, Wilk R. Ruiz, Arturo Morales-Pizarro, R. Rafael-Rutte, James Tirado-Lara, Manuel Saucedo-Bazalar, César Tuesta-Albán, Silverio Apaza-Apaza, Kathia K. Teodor
The banana (Musa sp.) is a very important crop in the socioeconomic development of Peru. The soft rot disease has been causing economic losses. The objective was to isolate and identify the causal agent of soft rot in banana and to implement management alternatives. Bacteria were isolated from pseudostem with soft rot symptoms from 11 producing areas in the Chira Valley. The pathogenicity tests were carried on pseudostem segments and banana shoots. The eight isolates were culturally, morphologically and biochemically characterized: All isolates were Gram negative, rod-shaped, with peritrichous flagella and colonies formed deep cavities on crystal violet pectate (CVP) medium. Biochemical tests showed a positive catalase reaction, gelatin hydrolysis, glucose and lactose fermentation, and tolerance to NaCl 5%. However, on TSI (Triple Iron Sugar) medium and erythromycin sensitivity only isolates A2M, A3Ma, A5Mall, A7VF, A9Sa and A10 reacted positively and isolates A6Q and A11Sal negatively. Growth at 28, 30, 36, 36, 37 and 45 °C was positive for all eight isolates, with the exception A6Q and A11Sal which did not develop at 45 °C. Cultural characteristics, biochemical and pathogenicity tests allowed to identify six isolates as Pectobacterium chrysanthemi and two as P. carotovorum subsp. carotovorum. Disease management is based on the use of certified suckers, adequate fertilization, organic matter applications, biocontrol agents and weevil insect control.
{"title":"Soft rot in organic banana pseudostem (Musasp):Symptomatology, cultural and biochemical characterization, pathogenicity,and management alternatives","authors":"R. Aguilar-Anccota, Wilk R. Ruiz, Arturo Morales-Pizarro, R. Rafael-Rutte, James Tirado-Lara, Manuel Saucedo-Bazalar, César Tuesta-Albán, Silverio Apaza-Apaza, Kathia K. Teodor","doi":"10.17268/sci.agropecu.2021.061","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2021.061","url":null,"abstract":"The banana (Musa sp.) is a very important crop in the socioeconomic development of Peru. The soft rot disease has been causing economic losses. The objective was to isolate and identify the causal agent of soft rot in banana and to implement management alternatives. Bacteria were isolated from pseudostem with soft rot symptoms from 11 producing areas in the Chira Valley. The pathogenicity tests were carried on pseudostem segments and banana shoots. The eight isolates were culturally, morphologically and biochemically characterized: All isolates were Gram negative, rod-shaped, with peritrichous flagella and colonies formed deep cavities on crystal violet pectate (CVP) medium. Biochemical tests showed a positive catalase reaction, gelatin hydrolysis, glucose and lactose fermentation, and tolerance to NaCl 5%. However, on TSI (Triple Iron Sugar) medium and erythromycin sensitivity only isolates A2M, A3Ma, A5Mall, A7VF, A9Sa and A10 reacted positively and isolates A6Q and A11Sal negatively. Growth at 28, 30, 36, 36, 37 and 45 °C was positive for all eight isolates, with the exception A6Q and A11Sal which did not develop at 45 °C. Cultural characteristics, biochemical and pathogenicity tests allowed to identify six isolates as Pectobacterium chrysanthemi and two as P. carotovorum subsp. carotovorum. Disease management is based on the use of certified suckers, adequate fertilization, organic matter applications, biocontrol agents and weevil insect control.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45949218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}