Pub Date : 2021-01-01DOI: 10.37279/2413-1725-2021-7-1-218-228
V. Chaban, I. Rudneva, V. GuskovaN., V. G. Shaida
Sakskoe Lake is the most thoroughly studied water body in Crimea. Its therapeutic mud and brine are exploited by balneological treatment methods. During the two centuries, when the lake was used by humans, it was subdivided into seven water bodies by dams, with two of these bodies (Eastern and Western) being now utilized for medical purposes, and the other five being protective. The Eastern (currently exploited) basin is now used to produce therapeutic mud and brine. The bottoms of the saline basins are covered by sediments: an upper layer of black silt, which is underlain by gray, steel-gray, occasionally bluish silt, which is used as therapeutic mud. The lake is fed by surface and groundwaters. Retaining dams and flood embankments erected in nearby ravines preclude desalination of the lake with flood and rain waters. Seawater is pumped to the lake through channels to preclude its drying. A quay is now actively constructed around the lake and will likely notably affect its hydrological and ecological conditions. The aim of the present study was the comparative analysis of the chemical and physical characteristics of the Eastern and Western basins of the Sakskoe Lake at the period of intensive building activity on their coasts at the summer period of 2019. Brine samples were collected at two tested sites in each of the lakes. Water salinity was measured by a PAL-06S LTA GO (Japan) refractometer and was expressed in ‰. The pH and Eh of the waters and oxygen dissolved in them were determined in the laboratory by an Expert-001 (Econix-Expert Moexa CoLtd, Russia) analyzer, with the use of appropriate Volta (Russia) selective electrodes. Ions concentration was determined spectrophotometrically and by atomic absorption method. Various living stages of Artemia populations in two water bodies were studied according the standard microscopic methods. The obtained results demonstrated, that the total content of the cations in the brine of the Western basin was in 1,5-fold higher as compared with the data of the brine in the Eastern basin (108,4 and 71,2 g/l respectively). The values were directly correlated with the high salinity of the Western basin brine. The identical trend was shown for the anions concentration, which was significantly greatly in the Western basin related to Eastern one (193,14 and 125,41 g/l correspondingly), which was connected with the different hydrogeological conditions of the formation of the ecosystems of the tested water bodies. At the other hand, pH, salinity and Eh of the brine in the Eastern basin were lower than in the brine of the Western basin, while the concentration of dissolved oxygen was higher. In the brine of the Eastern basin of the lake all living stages of Artemia, including cysts, nauplia and adults were found, while in the Western basin only cysts were shown. Therefore, according the obtained results we could suggest, that anthropogenic processes which were taken place on the coasts of the Sakskoe Lake accompani
{"title":"ECOSYSTEMS STATE OF EASTERN AND WESTERN BASINS OF SAKSKOE LAKE (CRIMEAN REPUBLIC)","authors":"V. Chaban, I. Rudneva, V. GuskovaN., V. G. Shaida","doi":"10.37279/2413-1725-2021-7-1-218-228","DOIUrl":"https://doi.org/10.37279/2413-1725-2021-7-1-218-228","url":null,"abstract":"Sakskoe Lake is the most thoroughly studied water body in Crimea. Its therapeutic mud and brine are exploited by balneological treatment methods. During the two centuries, when the lake was used by humans, it was subdivided into seven water bodies by dams, with two of these bodies (Eastern and Western) being now utilized for medical purposes, and the other five being protective. The Eastern (currently exploited) basin is now used to produce therapeutic mud and brine. The bottoms of the saline basins are covered by sediments: an upper layer of black silt, which is underlain by gray, steel-gray, occasionally bluish silt, which is used as therapeutic mud. The lake is fed by surface and groundwaters. Retaining dams and flood embankments erected in nearby ravines preclude desalination of the lake with flood and rain waters. Seawater is pumped to the lake through channels to preclude its drying. A quay is now actively constructed around the lake and will likely notably affect its hydrological and ecological conditions. The aim of the present study was the comparative analysis of the chemical and physical characteristics of the Eastern and Western basins of the Sakskoe Lake at the period of intensive building activity on their coasts at the summer period of 2019. Brine samples were collected at two tested sites in each of the lakes. Water salinity was measured by a PAL-06S LTA GO (Japan) refractometer and was expressed in ‰. The pH and Eh of the waters and oxygen dissolved in them were determined in the laboratory by an Expert-001 (Econix-Expert Moexa CoLtd, Russia) analyzer, with the use of appropriate Volta (Russia) selective electrodes. Ions concentration was determined spectrophotometrically and by atomic absorption method. Various living stages of Artemia populations in two water bodies were studied according the standard microscopic methods. The obtained results demonstrated, that the total content of the cations in the brine of the Western basin was in 1,5-fold higher as compared with the data of the brine in the Eastern basin (108,4 and 71,2 g/l respectively). The values were directly correlated with the high salinity of the Western basin brine. The identical trend was shown for the anions concentration, which was significantly greatly in the Western basin related to Eastern one (193,14 and 125,41 g/l correspondingly), which was connected with the different hydrogeological conditions of the formation of the ecosystems of the tested water bodies. At the other hand, pH, salinity and Eh of the brine in the Eastern basin were lower than in the brine of the Western basin, while the concentration of dissolved oxygen was higher. In the brine of the Eastern basin of the lake all living stages of Artemia, including cysts, nauplia and adults were found, while in the Western basin only cysts were shown. Therefore, according the obtained results we could suggest, that anthropogenic processes which were taken place on the coasts of the Sakskoe Lake accompani","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84215163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37279/2413-1725-2020-6-3-138-145
E. Nagaeva, E. Biryukova, N. S. Yarmolyuk, N. P. Mishin, E. E. Seidosmanova, V. Derkach, S. S. Peredkov
Modern research has proven the relationship between postural control and performance in healthy individuals who regularly engage in physical or athletic activity. Randomized studies on athletes have shown a positive relationship between performance and posture control. Some authors note that regular balance exercises significantly improve postural balance according to the dose-response ratio. At the same time, the description of the relationship between physical activity and postural balance mainly concerns adult athletes, while experimental work on the system of given posture control in primary school-age children, including athletes, is currently insufficient. In our opinion, a detailed study of the system of control of a given posture in children of primary school age, including sportsmen-wrestlers, allows us to evaluate the development of their coordination abilities and can be used later as a predictive criterion of competitive success of these sportsmen. In this connection, the purpose of this study was to study the system of control of a given position in young martial arts at the stage of initial sports training by means of computer stabilometry. In a study of the main stabilizing characteristics in the Romberg sample with open eyes, the examined children engaged in karate registered the smallest values of statokinesiograms length by 26.07 % (p<0.05), area by 64.82 % (p<0.05), and the index of work on ODC movement – by 29.29 % (p<0.05) compared to the control. The data received by us testifies to the fact that the sportsmen who are engaged in karate have the most economical mechanisms to maintain the given posture in comparison with the sportsmen of other groups, as well as those who are not engaged in sports. Such changes in the system of maintaining a given position are due to the fact that at an early stage of the training process in karate, special attention is paid to the development of coordination abilities, improvement of proprioceptive sensitivity and accuracy of position registration. Children engaged in Thai boxing also had lower stability scores than children in the control group: statokinesiogram length by 21.49 % (p<0.05), area by 30.76 % (p>0.05), and ODC movement score by 33.99 % (p<0.05). Probably, such changes are connected with the development of coordination of tayboxers by imitation exercises: elementary and arbitrary (shadow fighting), which are based on the elements of movement, impact and defense techniques. Young athletes engaged in taekwondo did not have reliable differences in computer stabilometry indices in comparison with the test subjects of the control group, which, probably, may be due to the peculiarities of their sports training, in particular, the priority in the development of their speed-force qualities over the control of static stability at the early stage of the training process.
{"title":"DEVELOPING THE ABILITY TO MAINTAIN BALANCE IN MARTIAL ARTISTS AT THE STAGE OF INITIAL SPORTS TRAINING","authors":"E. Nagaeva, E. Biryukova, N. S. Yarmolyuk, N. P. Mishin, E. E. Seidosmanova, V. Derkach, S. S. Peredkov","doi":"10.37279/2413-1725-2020-6-3-138-145","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-3-138-145","url":null,"abstract":"Modern research has proven the relationship between postural control and performance in healthy individuals who regularly engage in physical or athletic activity. Randomized studies on athletes have shown a positive relationship between performance and posture control. Some authors note that regular balance exercises significantly improve postural balance according to the dose-response ratio. At the same time, the description of the relationship between physical activity and postural balance mainly concerns adult athletes, while experimental work on the system of given posture control in primary school-age children, including athletes, is currently insufficient. In our opinion, a detailed study of the system of control of a given posture in children of primary school age, including sportsmen-wrestlers, allows us to evaluate the development of their coordination abilities and can be used later as a predictive criterion of competitive success of these sportsmen. In this connection, the purpose of this study was to study the system of control of a given position in young martial arts at the stage of initial sports training by means of computer stabilometry. In a study of the main stabilizing characteristics in the Romberg sample with open eyes, the examined children engaged in karate registered the smallest values of statokinesiograms length by 26.07 % (p<0.05), area by 64.82 % (p<0.05), and the index of work on ODC movement – by 29.29 % (p<0.05) compared to the control. The data received by us testifies to the fact that the sportsmen who are engaged in karate have the most economical mechanisms to maintain the given posture in comparison with the sportsmen of other groups, as well as those who are not engaged in sports. Such changes in the system of maintaining a given position are due to the fact that at an early stage of the training process in karate, special attention is paid to the development of coordination abilities, improvement of proprioceptive sensitivity and accuracy of position registration. Children engaged in Thai boxing also had lower stability scores than children in the control group: statokinesiogram length by 21.49 % (p<0.05), area by 30.76 % (p>0.05), and ODC movement score by 33.99 % (p<0.05). Probably, such changes are connected with the development of coordination of tayboxers by imitation exercises: elementary and arbitrary (shadow fighting), which are based on the elements of movement, impact and defense techniques. Young athletes engaged in taekwondo did not have reliable differences in computer stabilometry indices in comparison with the test subjects of the control group, which, probably, may be due to the peculiarities of their sports training, in particular, the priority in the development of their speed-force qualities over the control of static stability at the early stage of the training process.","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84836597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37279/2413-1725-2021-7-1-57-63
V. Eremenko, E. G. Rotmistrovskya
The paper presents the results of a study of the dynamics of testosterone and cortisol in 40 heifers of different breeds (Holstein black-and-white breed, Simmental breed, Aberdeen-Angus breed, crossbred animals. 10 heads from each breed.All experimental groups of heifers were analogous in age and term of pregnancy. The conditions for raising animals were the same and corresponded to their zootechnical feeding standards. The hormones cortisol and testosterone were determined by the enzyme immunoassay. The results of the conducted studies showed that before insemination, a slightly higher concentration of testosterone was observed in crossbred animals (Aberdeen-Angus x Simmental) and was 4.7±0.4 nmol/l. This indicator was relatively lower in black – and-white heifers and was 3.6±0.4 nmol/l. Significant changes in it were observed at 5 months of pregnancy.By the 5th month of pregnancy, the concentration of testosterone in all experimental groups increased approximately 2 times. There is a significant difference between crossbreeds, Simmental and black-and-white breeds at 5 months of pregnancy (р<0.05). More pronounced interbreeding differences were observed from the 6th month of pregnancy to the last month of pregnancy. At 6, 7, 8, 9 months of pregnancy between black – and-white heifers and crossbred animals, the testosterone level was statistically significant (р<0.05). Interbreed differences in the level of testosterone in the blood of experimental animals indicate the features of the testosterone-synthesizing system of different breeds, which is probably determined at the gene level. An increase in the concentration of testosterone in the blood of heifers indicates its participation in the processes of fetal formation and the reaction of the testosterone-synthesizing system of the mother’s body to the course of pregnancy. Differences in the level of testosterone in the blood of Black-and-white, Simmental, Aberdeen-Angus breeds and crossbreeds indicate the breed characteristics of the testosterone-synthesizing system, which is possibly determined at the gene level. Changes in cortisol concentration were similar to changes in testosterone. It should be noted that the highest levels of cortisol in the second half of pregnancy were consistently maintained in cross-bred heifers in relation to the black-and-white and Simmental breeds. At the 9th month of pregnancy, local weeks were statistically significantly higher than black-and-white and Simmental weeks (р<0.05). During pregnancy, the level of cortisol in the blood of heifers had ambiguous indicators and depended on the month of pregnancy and the breed affiliation of the animals. The increase in cortisol in the blood during pregnancy is obviously due to the effect of estrogens on the female body. Thus, we believe that as a result of pregnancy, the synthesis of α-glycoprotein increases, which binds to cortisol, contributing to an increase in its life in the blood and contributing to an increase in th
本文介绍了40头不同品种的小母牛(荷尔斯坦黑白品种、西蒙塔尔品种、阿伯丁-安格斯品种、杂交动物)睾酮和皮质醇的动态研究结果。每个品种10头。各试验组母牛的胎龄和妊娠期相近。饲养动物的条件相同，符合动物技术饲养标准。采用酶免疫分析法测定皮质醇和睾酮激素。结果表明，杂交动物(Aberdeen-Angus x Simmental)在授精前睾酮浓度略高，为4.7±0.4 nmol/l。该指标在黑白小母牛中相对较低，为3.6±0.4 nmol/l。在怀孕5个月时观察到明显的变化。妊娠第5个月时，各实验组睾酮浓度均升高约2倍。杂交品种、西门塔尔品种和黑白品种在妊娠5个月时差异显著(p <0.05)。从怀孕第6个月到怀孕最后一个月，观察到更明显的杂交差异。在妊娠6、7、8、9个月时，黑白小母牛与杂交动物的睾酮水平差异有统计学意义(p <0.05)。实验动物血液中睾酮水平的杂交差异表明了不同品种睾酮合成系统的特点，这可能是在基因水平上决定的。小母牛血液中睾酮浓度的增加表明它参与了胎儿形成过程和母体体内睾酮合成系统对妊娠过程的反应。黑白犬、西芒特犬、阿伯丁-安格斯犬和杂交犬血液中睾酮水平的差异表明了睾酮合成系统的品种特征，这可能是在基因水平上决定的。皮质醇浓度的变化与睾酮的变化相似。值得注意的是，与黑白和西门塔尔品种相比，杂交小母牛在怀孕后半期的皮质醇水平一直保持在最高水平。妊娠第9个月时，局部周数明显高于黑白周数和西门塔尔周数(p <0.05)。在怀孕期间，小母牛血液中的皮质醇水平有模糊的指标，取决于怀孕的月份和动物的品种隶属关系。怀孕期间血液中皮质醇的增加显然是由于雌激素对女性身体的影响。因此，我们认为，由于怀孕，α-糖蛋白的合成增加，与皮质醇结合，导致其在血液中的寿命增加，并导致总皮质醇浓度增加。
{"title":"DYNAMICS OF CORTISOL AND TESTOTERON IN THE BLOOD OF HEIFERS OF DIFFERENT BREEDS","authors":"V. Eremenko, E. G. Rotmistrovskya","doi":"10.37279/2413-1725-2021-7-1-57-63","DOIUrl":"https://doi.org/10.37279/2413-1725-2021-7-1-57-63","url":null,"abstract":"The paper presents the results of a study of the dynamics of testosterone and cortisol in 40 heifers of different breeds (Holstein black-and-white breed, Simmental breed, Aberdeen-Angus breed, crossbred animals. 10 heads from each breed.All experimental groups of heifers were analogous in age and term of pregnancy. The conditions for raising animals were the same and corresponded to their zootechnical feeding standards. The hormones cortisol and testosterone were determined by the enzyme immunoassay. The results of the conducted studies showed that before insemination, a slightly higher concentration of testosterone was observed in crossbred animals (Aberdeen-Angus x Simmental) and was 4.7±0.4 nmol/l. This indicator was relatively lower in black – and-white heifers and was 3.6±0.4 nmol/l. Significant changes in it were observed at 5 months of pregnancy.By the 5th month of pregnancy, the concentration of testosterone in all experimental groups increased approximately 2 times. There is a significant difference between crossbreeds, Simmental and black-and-white breeds at 5 months of pregnancy (р<0.05). More pronounced interbreeding differences were observed from the 6th month of pregnancy to the last month of pregnancy. At 6, 7, 8, 9 months of pregnancy between black – and-white heifers and crossbred animals, the testosterone level was statistically significant (р<0.05). Interbreed differences in the level of testosterone in the blood of experimental animals indicate the features of the testosterone-synthesizing system of different breeds, which is probably determined at the gene level. An increase in the concentration of testosterone in the blood of heifers indicates its participation in the processes of fetal formation and the reaction of the testosterone-synthesizing system of the mother’s body to the course of pregnancy. Differences in the level of testosterone in the blood of Black-and-white, Simmental, Aberdeen-Angus breeds and crossbreeds indicate the breed characteristics of the testosterone-synthesizing system, which is possibly determined at the gene level. Changes in cortisol concentration were similar to changes in testosterone. It should be noted that the highest levels of cortisol in the second half of pregnancy were consistently maintained in cross-bred heifers in relation to the black-and-white and Simmental breeds. At the 9th month of pregnancy, local weeks were statistically significantly higher than black-and-white and Simmental weeks (р<0.05). During pregnancy, the level of cortisol in the blood of heifers had ambiguous indicators and depended on the month of pregnancy and the breed affiliation of the animals. The increase in cortisol in the blood during pregnancy is obviously due to the effect of estrogens on the female body. Thus, we believe that as a result of pregnancy, the synthesis of α-glycoprotein increases, which binds to cortisol, contributing to an increase in its life in the blood and contributing to an increase in th","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85343678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37279/2413-1725-2020-6-3-262-274
I. Cheretaev, M. Ravaeva, E. Chuyan, V. F. Shulgin
The article presents the results of evaluation of analgesic and anti-inflammatory activity of the adduct of 1-hydroxy-1,1-ethylidendiphosphonic acid and bis(2-pyridyl-1,2,4-triazolyl-3)propane (HEA+BPP) in male rats with a 21-day course administration. The aim of the study was to evaluate analgesic and anti-inflammatory activity in male rats with 21-day course administration of HEA+BPP course administration with 21-day course administration. The animals were divided into 7 groups of 10 individuals. Analgesic activity of HEA+BPP was tested on 40 males, and anti – inflammatory activity was tested on 30 others. In studies of analgesic activity of HEA+BPP, males of one group were control and received intraperitoneal injections of 0.2 ml of saline solution every day at 10.00 for 21 days, the others groups received 0.2 ml of intraperitoneal injections of HEA+BPP at doses of 5, 50 and 100 mg/kg at the same time for 21 days. In analgesic studies, groups of animals were similarly formed, when testing the anti-inflammatory effect of HEA+BPP, only without injecting a dose of 100 mg/kg. HEA+BPP synthesized at the Department of General and inorganic chemistry of the faculty of biology and chemistry of the Tauride Academy (structural division) of the V. I. Vernadsky Crimean Federal University. The chemical purity of HEA+BPP was at least 98 %. The analgesic activity of HEA+BPP was evaluated by the parameters of pain sensitivity of animals in the background and 1 hour after intraperitoneal injections on the 1st, 6th, 10th, 14th and 21st days of the experiment in the models of acute pain stress «tail-flick», Randall-Sellitto and «hot plate». Before the tail-flick and Randall-Sellitto tests were performed, the animals were placed in special rat retainers after injection. The anti-inflammatory activity of HEA+BPP was evaluated in the formalin paw edema test in rats. Acute inflammatory response was reproduced by subplantar injection of 0.1 ml of 2 % formalin solution in the form of an aqueous solution into the left hind leg of a rat, and then the value of limb edema was measured using the method of water plethysmometry on the 10th and 21st days of course administration of HEA+BPP in doses of 5 and 50 mg/kg. The data are presented as mean and standard error mean, statistical analysis performed using the software packages StatSoft/STATISTICA 8 and Graph Pad Prism 7.0. The significance of differences between groups was determined using one-way analysis of variance (ANOVA) with post hoc test Tukey and non-parametric criterion of multiple comparisons of Dunn, according to official methodological recommendations on statistical treatment of the results of preclinical studies of medicines, given in the literature. During course administration to male rats of HEA+BPP for 21 days in the dose range from 5 to 100 mg / kg, it was found that this compound mainly demonstrates effectiveness as an analgesic with a single injection, only for mechanical pain, this substance is effecti
{"title":"EVALUATION OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF 1-HYDROXY-1,1-ETHYLIDENDIPHOSPHONIC ACID ADDUCT AND\u0000BIS (2-PYRIDYL-1,2,4-TRIAZOLYL-3)PROPANE DURING COURSE ADMINISTRATION IN MALE RATS","authors":"I. Cheretaev, M. Ravaeva, E. Chuyan, V. F. Shulgin","doi":"10.37279/2413-1725-2020-6-3-262-274","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-3-262-274","url":null,"abstract":"The article presents the results of evaluation of analgesic and anti-inflammatory activity of the adduct of 1-hydroxy-1,1-ethylidendiphosphonic acid and bis(2-pyridyl-1,2,4-triazolyl-3)propane (HEA+BPP) in male rats with a 21-day course administration. The aim of the study was to evaluate analgesic and anti-inflammatory activity in male rats with 21-day course administration of HEA+BPP course administration with 21-day course administration. The animals were divided into 7 groups of 10 individuals. Analgesic activity of HEA+BPP was tested on 40 males, and anti – inflammatory activity was tested on 30 others. In studies of analgesic activity of HEA+BPP, males of one group were control and received intraperitoneal injections of 0.2 ml of saline solution every day at 10.00 for 21 days, the others groups received 0.2 ml of intraperitoneal injections of HEA+BPP at doses of 5, 50 and 100 mg/kg at the same time for 21 days. In analgesic studies, groups of animals were similarly formed, when testing the anti-inflammatory effect of HEA+BPP, only without injecting a dose of 100 mg/kg. HEA+BPP synthesized at the Department of General and inorganic chemistry of the faculty of biology and chemistry of the Tauride Academy (structural division) of the V. I. Vernadsky Crimean Federal University. The chemical purity of HEA+BPP was at least 98 %. The analgesic activity of HEA+BPP was evaluated by the parameters of pain sensitivity of animals in the background and 1 hour after intraperitoneal injections on the 1st, 6th, 10th, 14th and 21st days of the experiment in the models of acute pain stress «tail-flick», Randall-Sellitto and «hot plate». Before the tail-flick and Randall-Sellitto tests were performed, the animals were placed in special rat retainers after injection. The anti-inflammatory activity of HEA+BPP was evaluated in the formalin paw edema test in rats. Acute inflammatory response was reproduced by subplantar injection of 0.1 ml of 2 % formalin solution in the form of an aqueous solution into the left hind leg of a rat, and then the value of limb edema was measured using the method of water plethysmometry on the 10th and 21st days of course administration of HEA+BPP in doses of 5 and 50 mg/kg. The data are presented as mean and standard error mean, statistical analysis performed using the software packages StatSoft/STATISTICA 8 and Graph Pad Prism 7.0. The significance of differences between groups was determined using one-way analysis of variance (ANOVA) with post hoc test Tukey and non-parametric criterion of multiple comparisons of Dunn, according to official methodological recommendations on statistical treatment of the results of preclinical studies of medicines, given in the literature. During course administration to male rats of HEA+BPP for 21 days in the dose range from 5 to 100 mg / kg, it was found that this compound mainly demonstrates effectiveness as an analgesic with a single injection, only for mechanical pain, this substance is effecti","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74355048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37279/2413-1725-2021-7-2-95-103
A. Mikhailova, A. Kaida, E. Eismont, V. Pavlenko
One of the reliable parameters for assessing the bioelectrical activity of the brain in children is the relative power (RP) of the EEG. Previous results have shown that EEG RP systematically changes not only depending on age, but also with various developmental disorders. The beta rhythm RP is of particular interest when performing tasks requiring the involvement of cognitive functions. In connection with the above, the purpose of the present study was to identify and analyze the RP of beta1 and beta2 EEG rhythms in a state of stable visual attention (SVА) in children 4–15 years old. The study involved 159 children (82 girls and 77 boys) aged 4–15 years with a normal level of physical and mental development. In the present study, children were required to observe and reproduce the movements of a computer mouse. At the same time, at certain moments, an image of a stationary computer mouse was presented, which was accompanied by a request to look at the computer screen, in connection with which both voluntary and involuntary attention of the subjects was activated. The use of the SVА situation makes it possible to analyze the nature of differences in the EEG pattern of children, taking into account the data on the neurophysiological mechanisms of attention. The greatest increase in the RP of the EEG beta1 rhythm found in the age range of 7–12. For the beta2 rhythm, the greatest increase discovered in the period of 10–15 years. The obtained values of the RP of the beta1 and beta2 rhythms of the EEG, recorded in the state of stable visual attention, in children of different ages can be used as reference data in the relevant research areas, as well as in the development of correction protocols for the cognitive functions and psychoemotional state using EEG biofeedback. The age-related dynamics of the beta rhythm RP can serve as an indicator of the maturation of cortical inhibition processes in children, which underlie the formation of voluntary cognitive functions and conscious purposeful behavior.
{"title":"AGE FEATURES OF BETA RHYTHMS EEG IN CHILDREN AGED 4-15 IN A STATE OF STABLE VISUAL ATTENTION","authors":"A. Mikhailova, A. Kaida, E. Eismont, V. Pavlenko","doi":"10.37279/2413-1725-2021-7-2-95-103","DOIUrl":"https://doi.org/10.37279/2413-1725-2021-7-2-95-103","url":null,"abstract":"One of the reliable parameters for assessing the bioelectrical activity of the brain in children is the relative power (RP) of the EEG. Previous results have shown that EEG RP systematically changes not only depending on age, but also with various developmental disorders. The beta rhythm RP is of particular interest when performing tasks requiring the involvement of cognitive functions. In connection with the above, the purpose of the present study was to identify and analyze the RP of beta1 and beta2 EEG rhythms in a state of stable visual attention (SVА) in children 4–15 years old. The study involved 159 children (82 girls and 77 boys) aged 4–15 years with a normal level of physical and mental development. In the present study, children were required to observe and reproduce the movements of a computer mouse. At the same time, at certain moments, an image of a stationary computer mouse was presented, which was accompanied by a request to look at the computer screen, in connection with which both voluntary and involuntary attention of the subjects was activated. The use of the SVА situation makes it possible to analyze the nature of differences in the EEG pattern of children, taking into account the data on the neurophysiological mechanisms of attention. The greatest increase in the RP of the EEG beta1 rhythm found in the age range of 7–12. For the beta2 rhythm, the greatest increase discovered in the period of 10–15 years. The obtained values of the RP of the beta1 and beta2 rhythms of the EEG, recorded in the state of stable visual attention, in children of different ages can be used as reference data in the relevant research areas, as well as in the development of correction protocols for the cognitive functions and psychoemotional state using EEG biofeedback. The age-related dynamics of the beta rhythm RP can serve as an indicator of the maturation of cortical inhibition processes in children, which underlie the formation of voluntary cognitive functions and conscious purposeful behavior.","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"278 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76572961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37279/2413-1725-2020-6-4-24-35
I. Bulavin
In the research works of various fields, including food industry and medicine, a great attention is paid to the medicinal and aromatic plants of the families Myrtaceae Juss., Lauraceae Juss. and Lamiaceae Martinov. Thymus L. is one of the most important genera of the Lamiaceae family, with aromatic and medicinal properties, it is one of the most popular plants with an essential oil, one of the ten best ones in the world. In the Nikita Botanical Gardens (NBG) on the ex situ collection plots, besides the introduced plants, there are thyme cultivars of the NBG selection. For further plant usage, a cultivar comprehensive study is necessary. Therefore, the objective of the research was a comparative morphological, anatomical analysis, an essential oil amount determination and a genetic similarity evaluation. T. mastichina (L.) L. cv. Svetliachok, T. striatus Vahl. cv. Jubileiniy and T. vulgaris L. cv. Fantazia (NBG selection) were used. The leaf morphology was studied under the stereomicroscope, the organ sections were obtained on a freezing microtome, stained with methylene blue and examined under a light microscope, an essential oil was extracted using Ginsberg devices, DNAs were isolated with CTAB-method, genetic similarity was evaluated with RAPD-PCR on the basis of binary matrices in the PAST program. A comparative morphological and anatomical analysis of the cultivars revealed the common structure of vegetative and generative organs. The differences were established in the stem color, the leaf blade sizes, the type of mechanical tissue under midrib, the area of inclusions with essential oil and its total amount. The analysis of a genetic similarity showed that T. mastichina cv. Svetliachok formed a separate cluster, cultivars of T. striatus (Jubileiniy) and T. vulgaris (Fantazia) were closer.
{"title":"A MORPHOLOGY, ANATOMY AND GENETIC CHARACTERIZATION OF SOME THYME CULTIVARS FROM THE NIKITA BOTANICAL GARDEN COLLECTION","authors":"I. Bulavin","doi":"10.37279/2413-1725-2020-6-4-24-35","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-4-24-35","url":null,"abstract":"In the research works of various fields, including food industry and medicine, a great attention is paid to the medicinal and aromatic plants of the families Myrtaceae Juss., Lauraceae Juss. and Lamiaceae Martinov. Thymus L. is one of the most important genera of the Lamiaceae family, with aromatic and medicinal properties, it is one of the most popular plants with an essential oil, one of the ten best ones in the world. In the Nikita Botanical Gardens (NBG) on the ex situ collection plots, besides the introduced plants, there are thyme cultivars of the NBG selection. For further plant usage, a cultivar comprehensive study is necessary. Therefore, the objective of the research was a comparative morphological, anatomical analysis, an essential oil amount determination and a genetic similarity evaluation. T. mastichina (L.) L. cv. Svetliachok, T. striatus Vahl. cv. Jubileiniy and T. vulgaris L. cv. Fantazia (NBG selection) were used. The leaf morphology was studied under the stereomicroscope, the organ sections were obtained on a freezing microtome, stained with methylene blue and examined under a light microscope, an essential oil was extracted using Ginsberg devices, DNAs were isolated with CTAB-method, genetic similarity was evaluated with RAPD-PCR on the basis of binary matrices in the PAST program. A comparative morphological and anatomical analysis of the cultivars revealed the common structure of vegetative and generative organs. The differences were established in the stem color, the leaf blade sizes, the type of mechanical tissue under midrib, the area of inclusions with essential oil and its total amount. The analysis of a genetic similarity showed that T. mastichina cv. Svetliachok formed a separate cluster, cultivars of T. striatus (Jubileiniy) and T. vulgaris (Fantazia) were closer.","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82855613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.37279/2413-1725-2020-6-2-204-213
G. A. Sevriukova, I. V. Khvastunova, I. Isupov
The article presents the results of a study on the cerebral hemodynamics of residents of the Volgograd region (165 respondents: 18–35 years old; 50–55 years; 56–60 years and over 61 years), making it possible to identify the parameters that are the most informative for assessing of blood supply disorders of the brain. Objective: to study the age features of cerebral blood circulation connected with breath phases. Evaluation of the functional state of the cerebral hemodynamics was carried out on the basis of comprehensive studies of blood supply to the brain (rest and against the background of respiratory test) using the method of reoencephalography. The reoencephalographic examination was performed in 4 leads (FM, OM left hemisphere; FM, OM right hemisphere). Indicators were registered: integral index of pulse filling, amplitude-frequency index, rheographic index, time of propagation of the rheographic wave, average vessel filling rate, venous outflow, dicrotic index, diastolic index. Decrease in total pulse blood filling of the brain at respondents of 50–55 years is revealed. In the dynamics of respiratory test myogenic reactions of vasoconstriction of resistive arteries of the brain in people of older age (50–55 years and older) are slightly expressed. The limit of vasoconstrictor reactions caused by the aging of the body is the cause of deterioration of the conditions of regional blood outflow. The inconsistency of the ratio of regional blood flow to its outflow is a marker of the reduction of adaptive reactions of the circulatory system of people over 50 years of age with an increase in venous pressure in the upper vena cava system. The information value, efficiency and safety of the reographic study at rest and against the background of respiratory test allows it to be recommended for inclusion in the plan of dispensary observations, especially those over 50 years of age.
{"title":"AGE SPECIFIC FEATURES OF REOENCEPHALOGRAPHY ASSOCIATED WITH RESPIRATORY PHASES","authors":"G. A. Sevriukova, I. V. Khvastunova, I. Isupov","doi":"10.37279/2413-1725-2020-6-2-204-213","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-2-204-213","url":null,"abstract":"The article presents the results of a study on the cerebral hemodynamics of residents of the Volgograd region (165 respondents: 18–35 years old; 50–55 years; 56–60 years and over 61 years), making it possible to identify the parameters that are the most informative for assessing of blood supply disorders of the brain. Objective: to study the age features of cerebral blood circulation connected with breath phases. Evaluation of the functional state of the cerebral hemodynamics was carried out on the basis of comprehensive studies of blood supply to the brain (rest and against the background of respiratory test) using the method of reoencephalography. The reoencephalographic examination was performed in 4 leads (FM, OM left hemisphere; FM, OM right hemisphere). Indicators were registered: integral index of pulse filling, amplitude-frequency index, rheographic index, time of propagation of the rheographic wave, average vessel filling rate, venous outflow, dicrotic index, diastolic index. Decrease in total pulse blood filling of the brain at respondents of 50–55 years is revealed. In the dynamics of respiratory test myogenic reactions of vasoconstriction of resistive arteries of the brain in people of older age (50–55 years and older) are slightly expressed. The limit of vasoconstrictor reactions caused by the aging of the body is the cause of deterioration of the conditions of regional blood outflow. The inconsistency of the ratio of regional blood flow to its outflow is a marker of the reduction of adaptive reactions of the circulatory system of people over 50 years of age with an increase in venous pressure in the upper vena cava system. The information value, efficiency and safety of the reographic study at rest and against the background of respiratory test allows it to be recommended for inclusion in the plan of dispensary observations, especially those over 50 years of age.","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76721066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.37279/2413-1725-2020-6-2-165-181
D. Nguyen, H. Dang, S. Kolesnikov, T. Ngo, V. MinnikovaT.
Ngoc Linh mountain range with Ngoc Linh peak of 2598m.a.s.l is an important part of forest ecosystems in Central – Highlands, Vietnam. Located on the complex terrain with stable and long-term geological components, the plant communities here contain the diversity and originality of the high mountains and contiguous areas between Central and Central Highlands Vietnam. The study area covers 65571 hectares, of which Ngoc Linh Nature Reserve covers about 40500 hectares, spread over medium and high mountain terrain. Strongly divided terrain has created the richness, diversity, and originality of ecosystems, especially the structure of natural vegetation. The article presents characteristics and differentiation of forest plantations in Ngoc Linh mountain area; study diversity, and conservation value of regional plant diversity resources; analyzing the characteristics and relationships of plants with geographical factors, especially the rules of elevation, slope, terrain direction, soil and drainage capacity of forest land. Comparison of similarities and differences with some other areas of the Central Highlands, especially the communities with the participation of coniferous plants. To implement the scientific content, various methods have been used. Among them are the main methods: Mapping method and Geographic Information Systems, Field survey methods and Phytogeography (Plant Geography) methods. The study results recorded 1128 species of vascular plants, belong to 627 genera, 175 families, 59 orders, 10 classes, 6 divisions. The flora of Ngoc Linh mountainous area fully converges the vascular plants of the plants in Vietnam, in which the species mainly focus on Magnoliophyta. This is the most diverse division with a total of 145 families, 571 genera and 1032 species, accounting for 82,39 % of families, 91,07 % of genera, and 91,48 % of species. The comparison results show that the proportion between sectors in the plants of Ngoc Linh and the plants of Vietnam has the same structure, with quite similar proportions of dominant species group. Accordingly, the absolute advantage of the Magnoliophyta is from 91.48 % and 92.62 % respectively. 80 rare and precious species have been identified in the IUCN Red List and Vietnam’s Red Data Book 2007. There are 189 endemic species of 135 genera of 66 families. In which there are 107 endemic species of Central Vietnam, the rest belong to Vietnam endemic. Some endemic species with high conservation significance is: Panax vietnamense, P. dalatensis, Quercus langbianensis. The rules of distribution according to elevation are shown in the structure of plant communities through species composition and canopy structure of the forest. Accordingly, plant communities have a high diversity with complex structures, including 3–4 layers. At 900 m.a.s.l elevation, in mixed forests, broadleaf trees form multi-dominant and diverse species. From 1000–1500 m.a.s.l, on the mountainside terrain, the dominant vegetation with P.
{"title":"PLANTS DIVERSITY AND FOREST STRUCTURE DIFFERENTIATION BY ELEVATION IN NGOC LINH MOUNTAIN RANGE, KON TUM PROVINCE, VIETNAM","authors":"D. Nguyen, H. Dang, S. Kolesnikov, T. Ngo, V. MinnikovaT.","doi":"10.37279/2413-1725-2020-6-2-165-181","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-2-165-181","url":null,"abstract":"Ngoc Linh mountain range with Ngoc Linh peak of 2598m.a.s.l is an important part of forest ecosystems in Central – Highlands, Vietnam. Located on the complex terrain with stable and long-term geological components, the plant communities here contain the diversity and originality of the high mountains and contiguous areas between Central and Central Highlands Vietnam. The study area covers 65571 hectares, of which Ngoc Linh Nature Reserve covers about 40500 hectares, spread over medium and high mountain terrain. Strongly divided terrain has created the richness, diversity, and originality of ecosystems, especially the structure of natural vegetation. The article presents characteristics and differentiation of forest plantations in Ngoc Linh mountain area; study diversity, and conservation value of regional plant diversity resources; analyzing the characteristics and relationships of plants with geographical factors, especially the rules of elevation, slope, terrain direction, soil and drainage capacity of forest land. Comparison of similarities and differences with some other areas of the Central Highlands, especially the communities with the participation of coniferous plants. To implement the scientific content, various methods have been used. Among them are the main methods: Mapping method and Geographic Information Systems, Field survey methods and Phytogeography (Plant Geography) methods. The study results recorded 1128 species of vascular plants, belong to 627 genera, 175 families, 59 orders, 10 classes, 6 divisions. The flora of Ngoc Linh mountainous area fully converges the vascular plants of the plants in Vietnam, in which the species mainly focus on Magnoliophyta. This is the most diverse division with a total of 145 families, 571 genera and 1032 species, accounting for 82,39 % of families, 91,07 % of genera, and 91,48 % of species. The comparison results show that the proportion between sectors in the plants of Ngoc Linh and the plants of Vietnam has the same structure, with quite similar proportions of dominant species group. Accordingly, the absolute advantage of the Magnoliophyta is from 91.48 % and 92.62 % respectively. 80 rare and precious species have been identified in the IUCN Red List and Vietnam’s Red Data Book 2007. There are 189 endemic species of 135 genera of 66 families. In which there are 107 endemic species of Central Vietnam, the rest belong to Vietnam endemic. Some endemic species with high conservation significance is: Panax vietnamense, P. dalatensis, Quercus langbianensis. The rules of distribution according to elevation are shown in the structure of plant communities through species composition and canopy structure of the forest. Accordingly, plant communities have a high diversity with complex structures, including 3–4 layers. At 900 m.a.s.l elevation, in mixed forests, broadleaf trees form multi-dominant and diverse species. From 1000–1500 m.a.s.l, on the mountainside terrain, the dominant vegetation with P.","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88486198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.37279/2413-1725-2020-6-2-267-280
E. Chuyan, I. Mironyuk, M. Ravaeva, I. Cheretaev, T. Grishina
The paper researches the effect of cobalt (АСCo2+) and zinc (АСZn2+), nickel (Ni2+) and manganese (Mn2+) acetylsalicylates in doses of 5 and 10 mg/kg on the characteristics of the rats’ cardiorespiratory system. The study was carried out on the basis of the Center for collective use of scientific equipment «Experimental Physiology and Biophysics» of the Department of Human and Animal Physiology and Biophysics of the V. I. Vernadsky Crimean Federal University. The research of the biological effect of the acetylsalicylic acid, АСCo2+, АСZn2+ , АСNi2+, АСMn2+was carried out during their intraperitoneal injection into rats in doses of 5 mg/kg and 10 mg/kg. Simultaneously the following characteristics of all the groups’ animals were registered: heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP) and diastolic blood pressure (DBP). The pulse pressure (PP) was calculated on the basis of the SBP and DBP indices difference. BP, HR and RR of the rats were registered with the help of the system NIBP200A («BiopacSystems, Inc.», USA). The results of the current research allowed to determine that the coordinating compounds of the acetylsalicylic acid with such metals as Co, Zn, Ni2+, Mn2+ not only have more pronounced biological effect in comparison with the acetylsalicylic acid, but demonstrate new properties; the most effect on the cardiorespiratory system was achieved by АСZn2+. Thus, the results of the current research allow us to claim that the salts of the acetylsalicylates, having the metal-complexing in its molecules’ composition, have more pronounced and qualitatively new properties in comparison with the acetylsalicylic acid precursor’s properties; this makes the further search for biological and pharmaceutical activity of these coordinating compounds more perspective, as well as it makes the technology of complexingan inexpensive and highly efficient approach to the creation of new medicines. The reported study was funded by RFBR, project number 20-33-70142
{"title":"INDICATORS OF THE RAT CARDIORESPIRATORY SYSTEM UNDER THE ACTION OF ACETYLSALICYLIC ACID AND ITS COMPLEX COMPOUNDS WITH METALS","authors":"E. Chuyan, I. Mironyuk, M. Ravaeva, I. Cheretaev, T. Grishina","doi":"10.37279/2413-1725-2020-6-2-267-280","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-2-267-280","url":null,"abstract":"The paper researches the effect of cobalt (АСCo2+) and zinc (АСZn2+), nickel (Ni2+) and manganese (Mn2+) acetylsalicylates in doses of 5 and 10 mg/kg on the characteristics of the rats’ cardiorespiratory system. The study was carried out on the basis of the Center for collective use of scientific equipment «Experimental Physiology and Biophysics» of the Department of Human and Animal Physiology and Biophysics of the V. I. Vernadsky Crimean Federal University. The research of the biological effect of the acetylsalicylic acid, АСCo2+, АСZn2+ , АСNi2+, АСMn2+was carried out during their intraperitoneal injection into rats in doses of 5 mg/kg and 10 mg/kg. Simultaneously the following characteristics of all the groups’ animals were registered: heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP) and diastolic blood pressure (DBP). The pulse pressure (PP) was calculated on the basis of the SBP and DBP indices difference. BP, HR and RR of the rats were registered with the help of the system NIBP200A («BiopacSystems, Inc.», USA). The results of the current research allowed to determine that the coordinating compounds of the acetylsalicylic acid with such metals as Co, Zn, Ni2+, Mn2+ not only have more pronounced biological effect in comparison with the acetylsalicylic acid, but demonstrate new properties; the most effect on the cardiorespiratory system was achieved by АСZn2+. Thus, the results of the current research allow us to claim that the salts of the acetylsalicylates, having the metal-complexing in its molecules’ composition, have more pronounced and qualitatively new properties in comparison with the acetylsalicylic acid precursor’s properties; this makes the further search for biological and pharmaceutical activity of these coordinating compounds more perspective, as well as it makes the technology of complexingan inexpensive and highly efficient approach to the creation of new medicines. The reported study was funded by RFBR, project number 20-33-70142","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74245064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.37279/2413-1725-2020-6-1-14-25
G. F. Allahverdiyeva, A. Asgarov
For the first time, micromorphology structure of seeds in 10 species (L. annuus, L. cicera, L. hirsutus, L. tuberosus, L. miniatus, L. pratensis, L. laxiflorus, L. aphaca, L. nissolia, L. sphaericus) belonging to sections (Lathyrus, Pratensis, Aphaca, Nissolia, Linearicarpus) of Lathyrus L. collected from various regions of Azerbaijan were analysed. Seed samples of 10 species were taken for analysis from different biotopes and different populations located away from one-another. Collected seed materials were gathered in special sterile paper bags and their moisture was dried with silicagel substance in laboratory conditions. During the research, morphological characters, as well as general shape, size and colour of seed, length and width of hilum were identified under Leica EZ4D stereomicroscope. The largest seeds have been measured in Lathyrus (L. cicera 4,4–5,0 mm) section and the smallest seeds in Nissolia (L. nissolia 1,8–2,3 mm) section. The longest hilum belongs to L. miniatus 1,8–2,8 mm and the smallest in L. Nissolia 0,4–0,7 mm. The width hilum was measured in L. annuus (0,7–1,0 mm) and the narrow hilum L. nissolia (0,1–0,3 mm). Mature seed (2–3) samples were selected from each type under the SEM, the seeds were placed on stools with double-sided adhesive tapes and covered with gold powder through the JEOL JFC1600 ion-spray device for 1 to 2 minutes. Seed samples were researched on the side surface. The photos of the surface of seeds were taken in a 3000× size in JEOL JSM6610 lv electronic microscope, and structural analysis of the different places of their surfaces was conducted. The results showed that the microscopic research of the surface of seed is of taxonomic importance and is used in specification of the status of sections. The morphological properties such as surface structure, hilum length and width, papillae features can be used to differentiate some sections and species, but seed size, general shape seed and hilum, seed colour are not characteristics can be used to differentiate some sections.
{"title":"MICROMORPHOLOGICAL STRUCTURE OF SEEDS IN SOME SPECIES OF LATHYRUS L. IN SOUTH CAUCASUS (AZERBAIJAN)","authors":"G. F. Allahverdiyeva, A. Asgarov","doi":"10.37279/2413-1725-2020-6-1-14-25","DOIUrl":"https://doi.org/10.37279/2413-1725-2020-6-1-14-25","url":null,"abstract":"For the first time, micromorphology structure of seeds in 10 species (L. annuus, L. cicera, L. hirsutus, L. tuberosus, L. miniatus, L. pratensis, L. laxiflorus, L. aphaca, L. nissolia, L. sphaericus) belonging to sections (Lathyrus, Pratensis, Aphaca, Nissolia, Linearicarpus) of Lathyrus L. collected from various regions of Azerbaijan were analysed. Seed samples of 10 species were taken for analysis from different biotopes and different populations located away from one-another. Collected seed materials were gathered in special sterile paper bags and their moisture was dried with silicagel substance in laboratory conditions. During the research, morphological characters, as well as general shape, size and colour of seed, length and width of hilum were identified under Leica EZ4D stereomicroscope. The largest seeds have been measured in Lathyrus (L. cicera 4,4–5,0 mm) section and the smallest seeds in Nissolia (L. nissolia 1,8–2,3 mm) section. The longest hilum belongs to L. miniatus 1,8–2,8 mm and the smallest in L. Nissolia 0,4–0,7 mm. The width hilum was measured in L. annuus (0,7–1,0 mm) and the narrow hilum L. nissolia (0,1–0,3 mm). Mature seed (2–3) samples were selected from each type under the SEM, the seeds were placed on stools with double-sided adhesive tapes and covered with gold powder through the JEOL JFC1600 ion-spray device for 1 to 2 minutes. Seed samples were researched on the side surface. The photos of the surface of seeds were taken in a 3000× size in JEOL JSM6610 lv electronic microscope, and structural analysis of the different places of their surfaces was conducted. The results showed that the microscopic research of the surface of seed is of taxonomic importance and is used in specification of the status of sections. The morphological properties such as surface structure, hilum length and width, papillae features can be used to differentiate some sections and species, but seed size, general shape seed and hilum, seed colour are not characteristics can be used to differentiate some sections.","PeriodicalId":21653,"journal":{"name":"Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84841277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：