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Effect of prechilling and exogenous gibberellin on seed germination of Primulina eburnea: a calcium-rich vegetable 预冷和外源赤霉素对富钙蔬菜报春花种子萌发的影响
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.01
Yi Zhang, Chuanhua Fu, Rongrong Zhou, Zi-Yu Liu, Chen Feng
Calcium-rich food and calcium supplements are expensive, especially for people in developing countries. Primulina eburnea is a calcium-rich vegetable with a high soluble calcium content in its leaves. In this study, we investigated the effects of prechilling and exogenous gibberellin on P. eburnea seed germination. Our results showed that both prechilling time and exogenous gibberellin affect P. eburnea seed germination. Three days prechilling increased germination percentage by 20.7-40.6%; mean germination time and time to first germination were reduced, indicating faster germination. A longer cold treatment enhanced the germination percentage but delayed the germination time. Prechilled P. eburnea seeds germinated 17.3-40.6% more when they were treated with GA3, but high GA3 did not shorten the germination time. Combination of three days prechilling and 50 ppm GA3 is the optimal treatment for P. eburnea seed germination. This research provides a powerful tool to accelerate breeding and cultivating this calcium-rich vegetable.
富含钙的食物和钙补充剂很昂贵,尤其是对发展中国家的人来说。报春花是一种富含钙的蔬菜,其叶片中可溶性钙含量高。本研究研究了预冷和外源赤霉素对赤霉素种子萌发的影响。结果表明,预冷时间和外源赤霉素均影响赤霉素种子的萌发。预冷3 d使发芽率提高20.7 ~ 40.6%;平均发芽时间和第一次发芽时间缩短,表明发芽速度加快。较长时间的冷处理提高了种子的发芽率,但延迟了种子的萌发时间。经GA3处理后,预冷后的青豆种子萌发率提高17.3% ~ 40.6%,但高GA3含量并没有缩短发芽时间。预冷3 d和50 ppm GA3的组合处理是对黑荆种子萌发的最佳处理。本研究为加快这种富钙蔬菜的育种和栽培提供了有力的工具。
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引用次数: 2
Seed priming stimulates germination and early seedling establishment of Corethrodendron multijugum under drought stress 在干旱胁迫下,种子激发能促进山楂萌发和早苗形成
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.06
ZhengSheng Li, Y. Liu
Seed priming can alleviate the negative effects of drought stress. The present study focused on exploring the effects of seed priming, drought and their interaction on the germination characteristics of Corethrodendron multijugum. We attempted to standardise an appropriate priming method for C. multijugum seeds by comparing the effects of various priming methods and drought stress on seed germination and seedling establishment to provide theoretical basis and technical support for subsequent cultivation and production. The results indicated that seed priming, drought stress and their interaction significantly (P < 0.01) affected all seed germination parameters and seedling establishment parameters, and germination was significantly (P < 0.05) correlated with early seedling establishment under drought stress. Of the different priming agents used in this study, water, 0.5% KMnO4, 0.3% H2O2 and 5% PEG-6000 significantly (P < 0.05) promoted seed germination and seedling establishment in C. multijugum. Among them, 0.5% KMnO4 was the most effective in alleviating the effects of drought stress on germination and early seedling establishment. Morphologically, the beneficial effects of priming can be attributed to increased seed germination, elongation and biomass accumulation of seedlings.
灌种可以缓解干旱胁迫带来的负面影响。本研究主要探讨了种子激发、干旱及其相互作用对合心石萌发特性的影响。通过比较不同的引种方式和干旱胁迫对多枣种子萌发和成苗的影响,试图规范多枣种子适宜的引种方法,为后续栽培生产提供理论依据和技术支持。结果表明:种子激发、干旱胁迫及其交互作用显著(P < 0.01)影响种子萌发参数和幼苗建立参数,干旱胁迫下种子萌发与幼苗早期建立极显著(P < 0.05)相关;在不同的激发剂中,水、0.5% KMnO4、0.3% H2O2和5% PEG-6000均显著(P < 0.05)促进了木参种子的萌发和成苗。其中,0.5% KMnO4对缓解干旱胁迫对种子萌发和早苗形成的影响最为有效。从形态上看,灌浆的有益作用可归因于种子萌发、伸长和幼苗生物量积累的增加。
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引用次数: 1
Alleviation of seed dormancy in kala zeera (Bunium persicum) through chilling and phytohormones 低温和植物激素对仙桃种子休眠的影响
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.2.03
R. Chauhan, Satbeer Singh, S. Pandey, Ajay Kumar, M. Kumari, Anil Kumar, A. Thakur, Sanatsujat Singh
Kala zeera (Bunium persicum) is a high-value medicinal spice plant. The seeds and extracts are used for culinary, perfumery, flavouring food and medicinal purposes. The commercial cultivation of kala zeera is hampered due to poor seed germination and deep physiological dormancy. To break the dormancy of kala zeera, three phytohormones, gibberellic acid (100 µmol L-1), 6-benzylaminopurine (10 µmol L-1) and thidiazuron (6.3 µmol L-1) were tested individually and in combination, under two temperature levels: continuous chilling at 4°C and one week chilling at 4°C followed by incubation at room temperature. The combined application of the three phytohormones under continuous chilling (4°C) improved the germination from 11.25 to 93.25% and reduced the mean germination time 60 to 50 days. The lowest mean germination time (34.78 days) and high mean daily germination (1.57 seeds day-1) with a high coefficient of germination velocity (2.88), were found under combined application of phytohormones as compared to their individual application. The findings have implications in successful propagation and systematic cultivation of this high-value medicinal spice plant.
花椒是一种高价值的药用香料植物。其种子和提取物可用于烹饪、香料、食品调味和药用。由于种子发芽差,生理休眠深,阻碍了卡拉的商业化种植。为了打破白僵菌的休眠,在4°C连续冷却和4°C冷却一周后室温孵育两种温度水平下,分别对赤霉素(100µmol L-1)、6-苄基氨基嘌呤(10µmol L-1)和噻脲(6.3µmol L-1)三种植物激素进行单独和联合测试。在连续降温(4°C)条件下,3种激素联合施用使种子的发芽率从11.25%提高到93.25%,平均发芽期缩短60 ~ 50 d。与单独施用相比,激素联合施用的平均发芽时间最短(34.78 d),平均日发芽率最高(1.57粒d -1),萌发速度系数最高(2.88)。研究结果对这种高价值药用香料植物的成功繁殖和系统栽培具有重要意义。
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引用次数: 0
Effect of saline treatment on seed germination of adzuki beans 生理盐水处理对小豆种子萌发的影响
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.04
Shuting Han, Kangyu Lu, Tianbing Guo, Ying Zhang, Binbin Wang, Baomei Wu
Salinity is one of the major causes of abiotic stress for plant development and growth worldwide, and affects crop yield significantly. To analyse the level of salinity tolerance among ‘Ji Hong 20’ (JH20), ‘Jin Xiao Dou 5’ (JXD5) and ‘Jin Xiao Dou 6’ (JXD6) adzuki bean cultivars, nine saline levels (0.0, 32.5, 65.0, 97.5, 130.0, 162.5, 195.0, 227.5 and 260.0 mmol L-1 NaCl) were applied during seed germination. Germination potentials and final germination percentages of all cultivars were significantly reduced by NaCl with the level at which germination was affected being dependent on cultivar. The JXD5 seeds had the highest germination index and vigour index at the same NaCl concentrations, followed by the JH20 and then JXD6 seeds. The shoot and root lengths of JH20 and JXD5 were significantly shorter at NaCl concentrations > 65.0 mmol L-1, while those of JXD6 were reduced at salinity > 32.5 mmol L-1. Quadratic regression models could well predict the germination percentage of JH20, JXD5 and JXD6 seeds under saline stress.
盐碱胁迫是影响植物生长发育的主要非生物胁迫因素之一,对作物产量有重要影响。为分析吉红20号(JH20)、晋小豆5号(JXD5)和晋小豆6号(JXD6)小豆品种的耐盐性,在种子萌发过程中施用了9个盐浓度(0.0、32.5、65.0、97.5、130.0、162.5、195.0、227.5和260.0 mmol L-1 NaCl)。NaCl处理显著降低了所有品种的萌发势和最终发芽率,且影响程度因品种而异。相同NaCl浓度下,JXD5种子萌发指数和活力指数最高,JH20次之,JXD6次之。JH20和JXD5的茎长和根长在盐浓度> 65.0 mmol L-1下显著缩短,而JXD6的茎长和根长在盐浓度> 32.5 mmol L-1下显著缩短。二次回归模型能较好地预测盐分胁迫下JH20、JXD5和JXD6种子的发芽率。
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引用次数: 0
Editorial: Seed Science and Technology. Volume 51 Issue 1 (2023) 社论:种子科技。第51卷第1期(2023年)
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.1.11
B. S. Olisa
Seed quality is the basis for differential crop productivity. Seed companies depend on the quality of the seeds for the seed trade, while farmers depend on the quality of the seeds to improve their productivity and secure their livelihood. Germination and varietal identification/purity are fundamental aspects of seed quality that are of prime importance to the global seed trade. Seed germination is influenced by several factors among which are the genetic factors and environmental cues of the prevailing growing conditions where the plant grows. Among these cues, climate change fundamentally affects crop development at all stages, starting from germination and seedling growth, to flowering and fruiting. Several methods have been formulated and validated for the evaluation of the varietal identification/purity for some crops, facilitate synchronised seed germination and enhance uniform field establishment for optimum crop yield in the face of climate change. This issue of Seed Science and Technology includes papers discussing the application of some of the methods, for example, prechilling and the use of exogenous gibberellin to stimulate germination, the use of tetrazolium as a quick viability test for rice seeds, the applied phenol test for varietal identification of rice seeds, and seed priming to enhance germination of Corethrodendron multijugum seeds. In addition, the physiological response of seed to soil salinity and storage environment conditions are also discussed.
种子质量是区分作物生产力的基础。种子公司依靠种子的质量进行种子贸易,而农民则依靠种子的质量来提高生产力和确保生计。发芽和品种鉴定/纯度是种子质量的基本方面,对全球种子贸易至关重要。种子萌发受多种因素的影响,其中包括遗传因素和植物生长的主要生长条件的环境因素。在这些因素中,气候变化从根本上影响作物从发芽和幼苗生长到开花和结果的各个阶段的发育。在气候变化的背景下,对某些作物的品种鉴定/纯度评价、促进种子同步萌发和加强统一的田间建设以获得最佳作物产量,已经制定并验证了几种方法。这一期的《种子科学与技术》包括讨论一些方法的应用,例如预冷和使用外源赤霉素刺激发芽,使用四氮唑作为水稻种子的快速活力试验,应用酚试验用于水稻种子的品种鉴定,以及种子激发以提高多核核种子的萌发。此外,还讨论了种子对土壤盐分和贮藏环境条件的生理反应。
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引用次数: 0
Seed dormancy and germination ecology of several clover species 几种三叶草种子休眠与萌发生态学研究
4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.06
Hamed Rezaei-Manesh, Farshid Ghaderi-Far, Iraj Nosratti, Asieh Siahmarguee, Bhagirath S. Chauhan
This research was carried out to evaluate the effect of (1) different methods of breaking seed dormancy (physical and chemical scarification, high temperature, hot water and after-ripening), and (2) environmental factors (temperature, salinity and water stress) on seed germination of Trifolium angustifolium , T. arvense , T. fragiferum , T. fucatum , T. repens , T. subterraneum and Trifolium sp. All experiments were conducted in the Seed Research Laboratory of Gorgan University of Agricultural Sciences and Natural Resources, Iran, in 2021. All the examined clover species were dormant after harvest, with little germination. Mechanical scarification with sandpaper or sulphuric acid resulted in more than 88 and 52&percnt; germination, respectively. Seeds were able to germinate between 5 and 35&deg;C, but the highest germination (&ge; 98&percnt; on average) occurred at 20&deg;C. Fluctuating temperature regimes were more efficient in improving the germination than constant temperatures. Seeds of clover species, in addition to having a hard seed coat, have a non-deep physiological dormancy. Fresh seeds showed a high sensitivity to water stress compared with after-ripened seeds. However, relative resistance to salinity stress was observed. The results obtained in this research can be used to develop effective sowing strategies and support the successful establishment of these clover species.
本文研究了(1)打破种子休眠的不同方法(物理和化学切割、高温、热水和催熟)和(2)环境因子(温度、盐度和水分胁迫)对三叶草(Trifolium angustifolium, T. arvense, T. fragiferum, T. fucatum, T. repens, T. repens)种子萌发的影响。所有实验均于2021年在伊朗戈尔根农业科学与自然资源大学种子研究实验室进行。所有被检查的三叶草品种在收获后都处于休眠状态,很少发芽。用砂纸或硫酸进行机械划伤的结果分别是88%和52%。分别发芽。种子在5 ~ 35℃之间能够发芽,但最高的发芽率为5 ~ 35℃。98年,percnt;(平均)发生在摄氏20度。波动温度比恒定温度更能有效地提高种子的萌发率。三叶草的种子除了有坚硬的种皮外,还有一个不深的生理休眠。鲜种子对水分胁迫的敏感性高于熟后种子。然而,观察到其对盐胁迫的相对抗性。本研究结果可用于制定有效的播种策略,并为三叶草品种的成功建立提供支持。
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引用次数: 0
Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions 克服野田菊种子休眠,改进基因库接入的活力监测方案
4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.12
Tamiru Olbana, Alice Muchugi, Yeshi Woldemariam, Fiona R. Hay, Nicholas Ndiwa, Chris S. Jones
Desmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage.
黄豆是一种多年生饲草豆科植物,用作牲畜饲料。这种植物的种子处于生理休眠状态,因此繁殖困难。休眠也影响了基因库中常规的生存力监测。本研究探讨了不同时间、不同地点采集的种子打破休眠的不同方法。结果表明,大多数预处理均能显著提高发芽率。用浓硫酸(25和30分钟)处理后再用砂纸刻蚀,正常苗的比例最高,分别为94.6%和96%。沸水(2.3分钟或3分钟)在除加入331外的所有加入中也有效。然而,用热水(3,5或8分钟)处理的材料有相当比例的硬种子。在所有材料中,热丝处理的种子死苗/异常苗的比例都很高,从27.5%(15489)到75.5%(16)不等。未经处理的种子在最终发芽计数时仍然坚硬。结果表明,利用浓硫酸和砂纸机械划伤等方法打破休眠,有利于提高种子萌发率,获得均匀的田间林分,有利于制定合理的贮藏决策。
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引用次数: 0
Divergent germination responses to fire-related cues of Suaeda glauca dimorphic seeds under salinity stress 盐碱化胁迫下酸枣二态种子萌发对火相关信号的不同响应
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.04
Dandan Zhao, Hongyuan Ma, Shaoyang Li, Wenwen Qi, Yi Li, Yaxiao Li, Mengdan Sun
Suaeda glauca is an annual herb in the family Amaranthaceae that produces dimorphic seeds (soft brown seeds and hard black seeds) on the same plant under natural conditions. Experiments were conducted to determine the salinity-alleviating effect of fire-related cues. Germination of both black and brown seeds was inhibited with an increase in salinity. Brown seeds had a higher germination percentage than black seeds in all conditions. Heat shock, smoke and their interaction had some effect in alleviating the salinity effect in brown seeds at 100-300 mM NaCl. This study provides valuable information to the understanding of the germination biology of Suaeda glauca.
Suaeda glauca是苋科一年生草本植物,在自然条件下在同一株植物上产生二态种子(软棕色种子和硬黑色种子)。实验确定了与火有关的线索减轻盐分的效果。随着盐度的增加,黑色和棕色种子的萌发都受到抑制。在所有条件下,棕色种子的发芽率都高于黑色种子。在100 ~ 300 mM NaCl条件下,热激、烟熏及其相互作用对缓解褐种子的盐效应有一定效果。本研究为了解紫田的萌发生物学提供了有价值的信息。
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引用次数: 0
Invited Review - Seed priming: what’s next? 特邀评论-种子启动:下一步是什么?
4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.3.10
Irfan Afzal
Seed priming is a pragmatic enhancement technology to improve seed performance used from ancient to modern times. Priming accelerates and synchronises the germination of seeds across a wider range of temperatures, giving rapid and uniform stand establishment of crop plants, particularly for low vigour and dormant seeds. Further investigation is necessary to extend the utilisation of low-cost and effective priming agents to a broader spectrum of species and to enhance availability. Seed priming is a cost-effective solution for small farmers as it reduces production costs and leads to uniform picking and harvesting, thus adding value in terms of quality. It is essential to scale up these approaches at both farmer and industry levels, particularly in developing countries. The benefits of priming are attributed to various putative molecular mechanisms such as epigenetic control of priming, germination, and dormancy-related attributes. However, in different species and cultivars, it remains elusive and there is a paucity of studies on the long-term benefits of re-drying after priming. This in-depth review summarises aspects of priming techniques in a modern context.
种子灌浆是一种实用的提高种子性能的技术,从古代到现代一直在使用。引种可以在更大的温度范围内加速和同步种子的萌发,使作物植物迅速和均匀地立苗,特别是对低活力和休眠的种子。进一步的研究是必要的,以扩大低成本和有效的引物剂的利用,以更广泛的物种和提高可得性。对小农来说,灌种是一种经济有效的解决方案,因为它降低了生产成本,并导致统一的采摘和收获,从而在质量方面增加了价值。必须在农民和工业两级,特别是在发展中国家,扩大这些办法的规模。启动的好处归因于各种假定的分子机制,如启动、萌发和休眠相关属性的表观遗传控制。然而,在不同的物种和栽培品种中,它仍然是难以捉摸的,并且缺乏对引水后再干燥的长期效益的研究。这篇深入的综述总结了在现代背景下启动技术的各个方面。
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引用次数: 0
SSR markers are useful tools in wheat varietal purity and genetic diversity assessment SSR标记是小麦品种纯度和遗传多样性评价的有效工具
IF 1.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.15258/sst.2023.51.2.01
Valentina Vasile, Adina Tîrziu, Elena Nicolae, M. Ciucă, C. Cornea
Assessing genetic similarity and purity degree among wheat cultivars is important in breeding programmes and SSR markers have all the features that make them suitable for this purpose. The aim of this study was to assess the varietal purity and genetic diversity among wheat cultivars grown in Romania using 22 SSR markers and to choose the most informative markers. The 40 samples included in the study consisted of seeds from certified and non-certified wheat cultivars, and from other related species. To establish that varietal purity is maintained, seeds from 14 certified wheat cultivars were analysed from two harvesting campaigns (years 2019 and 2020) and seeds of four of them were also compared with the authors’ seeds. The most polymorphic SSR marker was Xwmc596 (seven alleles), followed by Xwmc603 and Xwmc474 (six); Xgwm71, Xbarc347 and Xbarc074 (five); Xgwm469, DuPw004, Xgwm155 and Xbarc184 (four); Xwmc418, Xbarc170, Xwmc533, DuPw167, DuPw115, Xgwm413 and Xgwm372 (three); and DuPw217, DuPw205, Xgwm003 and Xgwm095 (two). SSR marker Xgwm052 showed low polymorphism (one). No contamination of the varieties was observed in these harvesting campaigns since all SSRs markers showed high similarity in the certified wheat cultivars harvest analysis; genetic diversity between genotypes was also observed.
小麦品种间的遗传相似性和纯度评估在育种规划中具有重要意义,而SSR标记具有适合这一目的的所有特征。本研究利用22个SSR标记对罗马尼亚小麦品种的纯度和遗传多样性进行了评价,并选择了信息量最大的标记。研究中包含的40个样品包括来自认证和非认证小麦品种以及其他相关品种的种子。为了确保保持品种纯度,在两个收获季节(2019年和2020年)对14个认证小麦品种的种子进行了分析,并将其中4个品种的种子与作者的种子进行了比较。多态性最高的SSR标记是Xwmc596(7个等位基因),其次是Xwmc603和Xwmc474(6个等位基因);Xgwm71、Xbarc347和Xbarc074 (5);Xgwm469、DuPw004、Xgwm155和Xbarc184(四);Xwmc418、Xbarc170、Xwmc533、DuPw167、DuPw115、Xgwm413和Xgwm372(三);DuPw217、DuPw205、Xgwm003、Xgwm095(2)。SSR标记Xgwm052多态性较低(1)。在这些收获活动中未观察到品种污染,因为所有SSRs标记在认证小麦品种收获分析中显示高度相似;基因型间也存在遗传多样性。
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引用次数: 0
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Seed Science and Technology
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