Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.3.15
Somya Hallan, Kumud Jarial, Rajender S. Jarial, Riya Dhiman, Rajina Khan
Bacterial leal spot is a common and destructive disease of cucurbits. Recent research revealed the seed borne nature of Xanthomonas cucurbitae . In this study, seeds wrapped in muslin cloth were dipped in various treatments involving hot water and chemicals. Hot water treatment of seeds at 55°C for both pumpkin and bottle gourd isolates resulted in minimum (28.73 and 23.33%, respectively) seedling infection. In vitro evaluation of fungicides and antibiotic (streptocycline) by using paper disc method revealed that Bordeaux mixture and copper oxychloride to be most effective against pumpkin and bottle gourd, respectively. Under chemical seed dip treatments, the minimum number of infected seedlings were recorded with streptocycline (37.64%) and copper oxychloride (40.80%) for pumpkin and bottlegourd, respectively. For both the isolates, maximum seed germination was recorded in seeds treated for 720 minutes (98.20 and 98.60%, respectively). A combination of streptocycline and copper oxychloride as seed treatment for three hours gave best results for both the isolates (16.33% and 15.67%, respectively). Under pot house conditions, combining streptocycline (100 ppm) and copper oxychloride (3000 ppm) as a seed dip treatment for three hours was found to be the most effective. The recorded minimum disease severity was 18.00 and 22.00%, respectively.
{"title":"Management of seed borne inoculum of <i>Xanthomonas cucurbiate</i> causing bacterial leaf spot of pumpkin and bottle gourd","authors":"Somya Hallan, Kumud Jarial, Rajender S. Jarial, Riya Dhiman, Rajina Khan","doi":"10.15258/sst.2023.51.3.15","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.15","url":null,"abstract":"Bacterial leal spot is a common and destructive disease of cucurbits. Recent research revealed the seed borne nature of Xanthomonas cucurbitae . In this study, seeds wrapped in muslin cloth were dipped in various treatments involving hot water and chemicals. Hot water treatment of seeds at 55&deg;C for both pumpkin and bottle gourd isolates resulted in minimum (28.73 and 23.33%, respectively) seedling infection. In vitro evaluation of fungicides and antibiotic (streptocycline) by using paper disc method revealed that Bordeaux mixture and copper oxychloride to be most effective against pumpkin and bottle gourd, respectively. Under chemical seed dip treatments, the minimum number of infected seedlings were recorded with streptocycline (37.64%) and copper oxychloride (40.80%) for pumpkin and bottlegourd, respectively. For both the isolates, maximum seed germination was recorded in seeds treated for 720 minutes (98.20 and 98.60%, respectively). A combination of streptocycline and copper oxychloride as seed treatment for three hours gave best results for both the isolates (16.33% and 15.67%, respectively). Under pot house conditions, combining streptocycline (100 ppm) and copper oxychloride (3000 ppm) as a seed dip treatment for three hours was found to be the most effective. The recorded minimum disease severity was 18.00 and 22.00%, respectively.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135103501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.13
Tim Loeffler
This issue of Seed Science and Technology includes several papers in which the authors have conducted seed vigour research on species where little to no previous research has been reported. Seed vigour is an important attribute of seed quality and the ISTA Vigour Committee encourages research in seed vigour on a wide range of species. It is important within seed laboratories and the seed industry to have validated vigour tests that are simple, easy and practical, and are correlated to field performance or seed longevity. A range of seed vigour tests were explored in the papers of this issue of SST with a focus on the single count radicle emergence (RE) test and / or mean germination time (MGT) for Chinese milk vetch, Festuca sinensis, cucurbit rootstocks and dill, with practical application for each specific crop. A paper using MGT and RE to evaluate emerging technologies using plasma seed priming treatments for maize is also discussed.
{"title":"Editorial: Seed Science and Technology. Volume 50 Issue 1 (2022)","authors":"Tim Loeffler","doi":"10.15258/sst.2022.50.1.13","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.13","url":null,"abstract":"This issue of Seed Science and Technology includes several papers in which the authors have conducted seed vigour research on species where little to no previous research has been reported. Seed vigour is an important attribute of seed quality and the ISTA Vigour Committee encourages research in seed vigour on a wide range of species. It is important within seed laboratories and the seed industry to have validated vigour tests that are simple, easy and practical, and are correlated to field performance or seed longevity. A range of seed vigour tests were explored in the papers of this issue of SST with a focus on the single count radicle emergence (RE) test and / or mean germination time (MGT) for Chinese milk vetch, Festuca sinensis, cucurbit rootstocks and dill, with practical application for each specific crop. A paper using MGT and RE to evaluate emerging technologies using plasma seed priming treatments for maize is also discussed.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.01
S. Ermis, Güleda Oktem, K. Mavi, F. Hay, I. Demir
A study was carried out to test whether a single count of radicle emergence (RE test) would correlate with the storage potential of seed lots of cucurbit (C. maxima × C. moschata) rootstock cultivars. The RE test was performed by counting radicle emergence percentage between 26 and 100 hours of germination. Seed longevity was determined by storage at 75% relative humidity at 35°C over 100 days, with seed survival curves constructed based on normal germination percentages. Ki (estimated initial viability in probits), σ (standard deviation of the normal distribution of seed death in time) and p50 (time for viability to fall to 50%) were determined through probit analysis. Correlation analysis showed that RE counts particularly between 30 and 34 hours were highly (P < 0.01) correlated with normal germination percentage, Ki and p50. It can be concluded that RE tests can be used as an indicator of subsequent seed lot longevity in cucurbit rootstock seed cultivars.
以瓜(C. maxima × C. moschata)砧木品种为研究对象，研究了单根出苗数(RE)与种子库存量的相关性。RE试验通过计算发芽26 ~ 100 h的胚根出苗率进行。通过在35°C 75%相对湿度条件下储存100天来测定种子寿命，并根据正常发芽率构建种子存活曲线。通过probit分析，确定了Ki(预估初始活力的probits)、σ(种子死亡时间正态分布的标准差)和p50(活力降至50%的时间)。相关分析表明，RE数与正常发芽率、Ki和p50呈极显著相关(P < 0.01)，其中30 ~ 34 h的RE数与正常发芽率、Ki和p50呈极显著相关。由此可见，稀土试验可作为葫芦砧木种子品种后续种子批次寿命的一个指标。
{"title":"The radicle emergence test and storage longevity of cucurbit rootstock seed lots","authors":"S. Ermis, Güleda Oktem, K. Mavi, F. Hay, I. Demir","doi":"10.15258/sst.2022.50.1.01","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.01","url":null,"abstract":"A study was carried out to test whether a single count of radicle emergence (RE test) would correlate with the storage potential of seed lots of cucurbit (C. maxima × C. moschata) rootstock cultivars. The RE test was performed by counting radicle emergence percentage between 26 and 100 hours of germination. Seed longevity was determined by storage at 75% relative humidity at 35°C over 100 days, with seed survival curves constructed based on normal germination percentages. Ki (estimated initial viability in probits), σ (standard deviation of the normal distribution of seed death in time) and p50 (time for viability to fall to 50%) were determined through probit analysis. Correlation analysis showed that RE counts particularly between 30 and 34 hours were highly (P < 0.01) correlated with normal germination percentage, Ki and p50. It can be concluded that RE tests can be used as an indicator of subsequent seed lot longevity in cucurbit rootstock seed cultivars.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.s.04
A. A. Powell
There have been many developments in seed vigour research and vigour testing since 2001 when seed vigour was first introduced into the ISTA Rules. This article will describe the seed ageing / repair hypothesis which has formed the basis of new vigour tests, how the hypothesis can explain differences in vigour and its use in vigour tests. The development and modification of ISTA validated vigour tests, and the extensive research that has examined the application of these tests to other species are described. Other approaches to vigour testing, based on seed response to stress, respiratory indices of vigour and the cell cycle are described and evaluated. The current and potential use of advanced technologies, such as image analysis, in vigour testing, and molecular research based on proteome analysis and DNA repair in Arabidopsis thaliana are described. The radicle emergence test, possibly using image analysis, is highlighted as having great potential for future application to a wide range of species. New approaches to vigour testing may be based on new technologies and molecular markers, but the need for rigorous evaluation of these approaches using commercial seed lots of crop species is emphasised.
{"title":"Invited Review: Seed vigour in the 21st century","authors":"A. A. Powell","doi":"10.15258/sst.2022.50.1.s.04","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.s.04","url":null,"abstract":"There have been many developments in seed vigour research and vigour testing since 2001 when seed vigour was first introduced into the ISTA Rules. This article will describe the seed ageing / repair hypothesis which has formed the basis of new vigour tests, how the hypothesis can explain differences in vigour and its use in vigour tests. The development and modification of ISTA validated vigour tests, and the extensive research that has examined the application of these tests to other species are described. Other approaches to vigour testing, based on seed response to stress, respiratory indices of vigour and the cell cycle are described and evaluated. The current and potential use of advanced technologies, such as image analysis, in vigour testing, and molecular research based on proteome analysis and DNA repair in Arabidopsis thaliana are described. The radicle emergence test, possibly using image analysis, is highlighted as having great potential for future application to a wide range of species. New approaches to vigour testing may be based on new technologies and molecular markers, but the need for rigorous evaluation of these approaches using commercial seed lots of crop species is emphasised.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"45 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.s.09
Steve K. Jones
It was an honour to be asked to provide an Editorial for the special 50th issue of Seed Science and Technology (SST). This special issue is a landmark for SST and ISTA. The 50th volume also means almost 50 years of seed research has been captured in the different volumes of SST with the first hard copy volume being issued in 1973. This was the same year that the supersonic airliner Concorde made its first non-stop trans-Atlantic crossing, the Sydney Opera house was opened and the first US space station (SkyLab) was launched.
{"title":"Editorial: Seed Science and Technology. Volume 50, Supplement (2022)","authors":"Steve K. Jones","doi":"10.15258/sst.2022.50.1.s.09","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.s.09","url":null,"abstract":"It was an honour to be asked to provide an Editorial for the special 50th issue of Seed Science and Technology (SST). This special issue is a landmark for SST and ISTA. The 50th volume also means almost 50 years of seed research has been captured in the different volumes of SST with the first hard copy volume being issued in 1973. This was the same year that the supersonic airliner Concorde made its first non-stop trans-Atlantic crossing, the Sydney Opera house was opened and the first US space station (SkyLab) was launched.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.3.09
Wei Han, M. Tanveer, Li Jiang, Lei Wang
Foxtail millet is a multipurpose crop and is widely cultivated in dry areas of Asia. The aim of this study was to investigate the effect of oligosaccharide priming on foxtail millet seeds during germination under osmotic stress. Germination percentage and seedling shoot and root lengths were not affected by 5% polyethylene glycol (PEG 6000). However, osmotic stress (15% and 25% PEG) dramatically decreased seed germination and other parameters. Under 15% PEG, pre-soaking seeds with low to moderate concentrations (0.1-10 mg L-1) of oligosaccharides had a positive effect on the germination percentage, germination index, shoot length and root length. Thus, the negative effects of osmotic stress on seed germination and seedling growth could be alleviated by oligosaccharide priming treatments, especially at a 1 mg L-1 concentration. These results suggest that oligosaccharides can dramatically improve the osmotic tolerance ability of foxtail millet seeds and seedlings.
{"title":"Pre-soaking foxtail millet seeds with oligosaccharides enhances germination and seedling growth under PEG-induced osmotic stress","authors":"Wei Han, M. Tanveer, Li Jiang, Lei Wang","doi":"10.15258/sst.2022.50.3.09","DOIUrl":"https://doi.org/10.15258/sst.2022.50.3.09","url":null,"abstract":"Foxtail millet is a multipurpose crop and is widely cultivated in dry areas of Asia. The aim of this study was to investigate the effect of oligosaccharide priming on foxtail millet seeds during germination under osmotic stress. Germination percentage and seedling shoot and root lengths were not affected by 5% polyethylene glycol (PEG 6000). However, osmotic stress (15% and 25% PEG) dramatically decreased seed germination and other parameters. Under 15% PEG, pre-soaking seeds with low to moderate concentrations (0.1-10 mg L-1) of oligosaccharides had a positive effect on the germination percentage, germination index, shoot length and root length. Thus, the negative effects of osmotic stress on seed germination and seedling growth could be alleviated by oligosaccharide priming treatments, especially at a 1 mg L-1 concentration. These results suggest that oligosaccharides can dramatically improve the osmotic tolerance ability of foxtail millet seeds and seedlings.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.s.02
C. Baskin, J. Baskin
Physiological dormancy (PD) is the most common class of seed dormancy on earth. It is highly variable and is an important part of the adaptation of many species to their habitat. Often, two questions are asked about seeds that failed to germinate in a germination test: (1) are they non-viable or dormant, and (2) if dormant do they have PD and how is it broken? We provide an overview on how to approach studies of species with PD for which few or no data are available. Information about habitat environmental conditions during the seed stage can be used to formulate hypotheses on dormancy-breaking and germination requirements. These hypotheses can be tested by a move-along experiment. There are three levels of PD, and they are distinguished by the conditions required to break PD and promote germination. Seeds with non-deep PD (the most common level of PD) require different conditions for dormancy-break and germination, i.e. a change in the season, while those with intermediate and deep PD germinate at the dormancy-breaking conditions, e.g. during a long period of cold stratification. Knowing how to break PD enhances testing for seed viability and helps ensure high germination percentages of viable seeds.
{"title":"Invited Review: Mimicking the natural thermal environments experienced by seeds to break physiological dormancy to enhance seed testing and seedling production","authors":"C. Baskin, J. Baskin","doi":"10.15258/sst.2022.50.1.s.02","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.s.02","url":null,"abstract":"Physiological dormancy (PD) is the most common class of seed dormancy on earth. It is highly variable and is an important part of the adaptation of many species to their habitat. Often, two questions are asked about seeds that failed to germinate in a germination test: (1) are they non-viable or dormant, and (2) if dormant do they have PD and how is it broken? We provide an overview on how to approach studies of species with PD for which few or no data are available. Information about habitat environmental conditions during the seed stage can be used to formulate hypotheses on dormancy-breaking and germination requirements. These hypotheses can be tested by a move-along experiment. There are three levels of PD, and they are distinguished by the conditions required to break PD and promote germination. Seeds with non-deep PD (the most common level of PD) require different conditions for dormancy-break and germination, i.e. a change in the season, while those with intermediate and deep PD germinate at the dormancy-breaking conditions, e.g. during a long period of cold stratification. Knowing how to break PD enhances testing for seed viability and helps ensure high germination percentages of viable seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.2.09
Serpil Mis, S. Ermis, A. A. Powell, I. Demir
This study was conducted to test the potential of the radicle emergence (RE) test to predict normal germination percentage (NG) in seed lots of watermelon, lettuce and carrot. One seed lot of each species was aged at 100% RH and 45°C for different times to produce 14 aged sub-samples. The RE (≥ 2 mm radicle) was counted during a germination test (ISTA Rules) and NG was assessed at the end of the test. RE counts after 74 (watermelon), 40 (lettuce) and 120 hours (carrot) were significantly (P < 0.001) related to NG (watermelon, R2 = 0.81; lettuce, R2 = 0.74; carrot, R2 = 0.81). RE counts at the same times were significantly related to NG in ten commercial seed lots of watermelon (R2 = 0.66, P < 0.01), nine lots of lettuce (R2 = 0.94, P < 0.001) and eight lots of carrot (R2 = 0.64, P < 0.05). The potential of the RE test to predict NG of both different lots within the same cultivar (watermelon, carrot) and lots from different cultivars (lettuce) is discussed.
通过对西瓜、生菜和胡萝卜种子批进行胚根出苗试验，研究了胚根出苗试验预测正常发芽率的潜力。在100% RH和45°C条件下，每个种属一个种子批次进行不同时间的陈化，得到14个陈化子样。在发芽试验中(ISTA规则)计数RE(≥2mm胚根)，并在试验结束时评估NG。西瓜74小时、生菜40小时、胡萝卜120小时后RE计数与NG显著相关(P < 0.001)(西瓜，R2 = 0.81;生菜，R2 = 0.74;胡萝卜，R2 = 0.81)。西瓜10个商品种子批次(R2 = 0.66, P < 0.01)、生菜9个批次(R2 = 0.94, P < 0.001)、胡萝卜8个批次(R2 = 0.64, P < 0.05)的RE同时计数与NG呈极显著相关。讨论了可重构检验预测同一品种不同批次(西瓜、胡萝卜)和不同品种批次(生菜)NG的潜力。
{"title":"Radicle emergence (RE) test identifies differences in normal germination percentages (NG) of watermelon, lettuce and carrot seed lots","authors":"Serpil Mis, S. Ermis, A. A. Powell, I. Demir","doi":"10.15258/sst.2022.50.2.09","DOIUrl":"https://doi.org/10.15258/sst.2022.50.2.09","url":null,"abstract":"This study was conducted to test the potential of the radicle emergence (RE) test to predict normal germination percentage (NG) in seed lots of watermelon, lettuce and carrot. One seed lot of each species was aged at 100% RH and 45°C for different times to produce 14 aged sub-samples. The RE (≥ 2 mm radicle) was counted during a germination test (ISTA Rules) and NG was assessed at the end of the test. RE counts after 74 (watermelon), 40 (lettuce) and 120 hours (carrot) were significantly (P < 0.001) related to NG (watermelon, R2 = 0.81; lettuce, R2 = 0.74; carrot, R2 = 0.81). RE counts at the same times were significantly related to NG in ten commercial seed lots of watermelon (R2 = 0.66, P < 0.01), nine lots of lettuce (R2 = 0.94, P < 0.001) and eight lots of carrot (R2 = 0.64, P < 0.05). The potential of the RE test to predict NG of both different lots within the same cultivar (watermelon, carrot) and lots from different cultivars (lettuce) is discussed.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"41 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.1.08
T. Doolotkeldieva, S. Bobusheva
Thirty-one species of vegetable seeds from Kyrgyzstan’s State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. Screening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 16S rRNA gene of diseased seeds was used to identify the plant pathogenic bacteria, which included Pseudomonas syringae, Pseudomonas savastanoi, Pseudomonas cerasi, Pectobacterium carotovorum, Xanthomonas oryzae, Erwinia sp. and others from the Proteobacteria phylum. For the coating of vegetable seeds, S. alfalfae CI-4 was selected. This strain had a beneficial double effect on the tested vegetable seeds at 1 × 103 spores mL-1.
{"title":"Microbial communities of vegetable seeds and biocontrol microbes for seed treatment","authors":"T. Doolotkeldieva, S. Bobusheva","doi":"10.15258/sst.2022.50.1.08","DOIUrl":"https://doi.org/10.15258/sst.2022.50.1.08","url":null,"abstract":"Thirty-one species of vegetable seeds from Kyrgyzstan’s State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. Screening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 16S rRNA gene of diseased seeds was used to identify the plant pathogenic bacteria, which included Pseudomonas syringae, Pseudomonas savastanoi, Pseudomonas cerasi, Pectobacterium carotovorum, Xanthomonas oryzae, Erwinia sp. and others from the Proteobacteria phylum. For the coating of vegetable seeds, S. alfalfae CI-4 was selected. This strain had a beneficial double effect on the tested vegetable seeds at 1 × 103 spores mL-1.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.15258/sst.2022.50.3.02
Huiliang Liu, Lingwei Zhang, Bo Liu
Seed germination is the key initial stage in the plant life cycle and strongly regulated by the external environment. Nitrogen deposition is an important environmental change factor that is increasing and affecting the structure and function of many ecosystems. This research aimed to investigate the effect of nitrogen deposition on the seed germination of nine ephemeral plants, including seed germination percentage, germination index and seed viability under four levels of nitrogen deposition. The germination percentage of the studied species differed significantly in response to nitrogen deposition, but nitrogen deposition has almost no significant effect on germination index. This study can provide information on the germination strategy of ephemeral plants in response to increasing nitrogen deposition in future, and the results can guide ecological conservation in arid areas.
{"title":"Effect of nitrogen deposition on seed germination of ephemeral species in the cold desert","authors":"Huiliang Liu, Lingwei Zhang, Bo Liu","doi":"10.15258/sst.2022.50.3.02","DOIUrl":"https://doi.org/10.15258/sst.2022.50.3.02","url":null,"abstract":"Seed germination is the key initial stage in the plant life cycle and strongly regulated by the external environment. Nitrogen deposition is an important environmental change factor that is increasing and affecting the structure and function of many ecosystems. This research aimed to investigate the effect of nitrogen deposition on the seed germination of nine ephemeral plants, including seed germination percentage, germination index and seed viability under four levels of nitrogen deposition. The germination percentage of the studied species differed significantly in response to nitrogen deposition, but nitrogen deposition has almost no significant effect on germination index. This study can provide information on the germination strategy of ephemeral plants in response to increasing nitrogen deposition in future, and the results can guide ecological conservation in arid areas.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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