This study compared the seed germination of 30 tree species from Central Amazonia using a wide temperature range (5‑40°C with 12 hours of light daily). Seeds were submitted to pre-germination treatments, whenever necessary, sown on vermiculite or germitest paper, and normal seedling development was assessed until stabilisation. With the same data set, seeds’ optimal germination temperature (T opt ) was determined by comparing the following approaches: (i) 2-step, the temperature with the highest germination and the shortest time to seed population reach 50% of germination was chosen; (ii) the highest Germination Speed Index (GSI); (iii) using the formula developed by Olff and collaborators (Olff’s approach); (iv) three species were evaluated with Covell’s approach; and (v) new formulas for T opt calculation are proposed, based on germination success and five different variables of germination rate (Cipriani’s approach). Comparing the results, T opt differed between 0.6-10.0°C. GSI and 2-step indicated generally higher values than Olff’s formula. The minimum and maximum T opt differences between Cipriani’s approaches were between 0.1-2.2°C. Our study showed that T opt should be determined for each species as, even the 30 Amazonian tree species of the same habitat, T opt ranged between 22.5 and 32.5°C.
{"title":"Thermal optima for seed germination of 30 tree species from Central Amazonia: a comparison of approaches and a new proposal","authors":"Vânia Beatriz Cipriani, Geângelo Petene Calvi, Isolde Dorothea Kossmann Ferraz","doi":"10.15258/sst.2023.51.3.13","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.13","url":null,"abstract":"This study compared the seed germination of 30 tree species from Central Amazonia using a wide temperature range (5‑40°C with 12 hours of light daily). Seeds were submitted to pre-germination treatments, whenever necessary, sown on vermiculite or germitest paper, and normal seedling development was assessed until stabilisation. With the same data set, seeds’ optimal germination temperature (T opt ) was determined by comparing the following approaches: (i) 2-step, the temperature with the highest germination and the shortest time to seed population reach 50% of germination was chosen; (ii) the highest Germination Speed Index (GSI); (iii) using the formula developed by Olff and collaborators (Olff’s approach); (iv) three species were evaluated with Covell’s approach; and (v) new formulas for T opt calculation are proposed, based on germination success and five different variables of germination rate (Cipriani’s approach). Comparing the results, T opt differed between 0.6-10.0°C. GSI and 2-step indicated generally higher values than Olff’s formula. The minimum and maximum T opt differences between Cipriani’s approaches were between 0.1-2.2°C. Our study showed that T opt should be determined for each species as, even the 30 Amazonian tree species of the same habitat, T opt ranged between 22.5 and 32.5°C.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.3.17
Carina Gallo, Luciana Magnano
This study was carried out to determine the use of single counts of radicle emergence to test seed vigour and estimate seedling field emergence of soybean ( Glycine max ) seed lots. Results of radicle emergence (RE) (production of 2 mm radicle) at 20°C for 48 hours and 25°C for 24 hours, mean germination time (MGT), tetrazolium vigour test (TZ) and field emergence (FE) were compared using nine soybean seed lots with standard germination above 80%. The single counts of RE performed at 20 and 25°C were significantly correlated with the MGT at the same temperature ( R 2 = 0.82 and 0.89, respectively) and with the TZ vigour ( R 2 = 0.96 and 0.93, respectively). The RE counts at both temperatures also estimated the field emergence of the seed lots ( R 2 = 0.74 at 20°C and 0.68 at 25°C). The TZ vigour results had a significant correlation with the seedling field emergence ( R 2 = 0.70). Results indicate that a single count of radicle emergence after 48 hours at 20°C or 24 hours at 25°C is an efficient option for soybean seed vigour assessment.
{"title":"Radicle emergence test for soybean vigour testing","authors":"Carina Gallo, Luciana Magnano","doi":"10.15258/sst.2023.51.3.17","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.17","url":null,"abstract":"This study was carried out to determine the use of single counts of radicle emergence to test seed vigour and estimate seedling field emergence of soybean ( Glycine max ) seed lots. Results of radicle emergence (RE) (production of 2 mm radicle) at 20°C for 48 hours and 25°C for 24 hours, mean germination time (MGT), tetrazolium vigour test (TZ) and field emergence (FE) were compared using nine soybean seed lots with standard germination above 80%. The single counts of RE performed at 20 and 25°C were significantly correlated with the MGT at the same temperature ( R 2 = 0.82 and 0.89, respectively) and with the TZ vigour ( R 2 = 0.96 and 0.93, respectively). The RE counts at both temperatures also estimated the field emergence of the seed lots ( R 2 = 0.74 at 20°C and 0.68 at 25°C). The TZ vigour results had a significant correlation with the seedling field emergence ( R 2 = 0.70). Results indicate that a single count of radicle emergence after 48 hours at 20°C or 24 hours at 25°C is an efficient option for soybean seed vigour assessment.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135563947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.1.02
Yuehua Wu, Laura Berríos-Ortiz, Ernest Allen, Todd Erickson, Elizabeth Stewart
Polyacrylamide isoelectric focusing electrophoresis (PA-IEF) provides alternative or supplementary testing for identifying the Kentucky 31 variety of tall fescue. Over a two-year period, field grow-out tests and a novel use of PA-IEF gel electrophoresis procedures were compared for 276 tall fescue samples. The PA-IEF gel test clearly identified a unique 4-band pattern in the Kentucky 31 variety, which was not detected in 21 other commercial varieties of tall fescue. This unique 4-band pattern can be used to differentiate between Kentucky 31 and other tall fescue varieties. Finally, the method provided results over a much shorter time frame than field grow-out tests.
{"title":"Polyacrylamide isoelectric focusing electrophoresis for differentiating Kentucky 31 tall fescue by esterase enzyme","authors":"Yuehua Wu, Laura Berríos-Ortiz, Ernest Allen, Todd Erickson, Elizabeth Stewart","doi":"10.15258/sst.2023.51.1.02","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.02","url":null,"abstract":"Polyacrylamide isoelectric focusing electrophoresis (PA-IEF) provides alternative or supplementary testing for identifying the Kentucky 31 variety of tall fescue. Over a two-year period, field grow-out tests and a novel use of PA-IEF gel electrophoresis procedures were compared for 276 tall fescue samples. The PA-IEF gel test clearly identified a unique 4-band pattern in the Kentucky 31 variety, which was not detected in 21 other commercial varieties of tall fescue. This unique 4-band pattern can be used to differentiate between Kentucky 31 and other tall fescue varieties. Finally, the method provided results over a much shorter time frame than field grow-out tests.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.1.08
N. Sukkaew, J. Kaewnaborn, W. Soonsuwon, V. Wongvarodom
Germination testing of rice seeds takes 14 days, while the tetrazolium test can characterise seed viability in hours. The goal of this study was to evaluate the viability of stored rice seeds cv. Chiang Phatthalung by a practical and efficient tetrazolium method. Seeds were stored at room temperature (28°C) and in cold storage (10°C) for 0, 3, 6, 9 and 12 months and then their viability and physiological quality was tested. Seeds of all treatments were soaked in water for 18 hours, cut longitudinally and immersed in 1% tetrazolium solution for two hours at 30°C according to the ISTA method, or in tetrazolium solution at different concentrations (0.0625, 0.125, 0.25 and 0.5%) and staining temperatures (30, 35, 40 and 45°C). The modified tetrazolium test with 0.125% tetrazolium concentration and staining at 40°C of stored rice seeds correlated positively with standard germination (r = 0.98, P < 0.01), soil seedling emergence (r = 0.98, P < 0.01), shoot length (r = 0.78, P < 0.01), root length (r = 0.81, P < 0.01) and seedling dry weight (r = 0.75, P < 0.01). Thus, this staining condition can be used to efficiently estimate the viability of freshly harvested and stored rice seeds.
水稻种子的发芽测试需要14天，而四氮唑测试可以在几个小时内确定种子的活力。本研究的目的是评价储藏水稻种子的活力。蒋法隆采用实用高效的四氮唑方法。将种子在室温(28°C)和冷藏(10°C)中分别保存0、3、6、9和12个月，并对其活力和生理品质进行检测。所有处理的种子在水中浸泡18小时，纵向切开，在1%四唑铵溶液中浸泡2小时，条件为30°C (ISTA法)，或在不同浓度(0.0625、0.125、0.25和0.5%)和染色温度(30、35、40和45°C)的四唑铵溶液中浸泡。改良四氮唑浓度为0.125%的四氮唑试验和40℃染色与标准发芽率(r = 0.98, P < 0.01)、土壤出苗率(r = 0.98, P < 0.01)、茎长(r = 0.78, P < 0.01)、根长(r = 0.81, P < 0.01)和幼苗干重(r = 0.75, P < 0.01)呈正相关。因此，这种染色条件可以用来有效地估计新鲜收获和储存的水稻种子的活力。
{"title":"Tetrazolium test for evaluating viability of stored rice (Oryza sativa) seeds","authors":"N. Sukkaew, J. Kaewnaborn, W. Soonsuwon, V. Wongvarodom","doi":"10.15258/sst.2023.51.1.08","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.08","url":null,"abstract":"Germination testing of rice seeds takes 14 days, while the tetrazolium test can characterise seed viability in hours. The goal of this study was to evaluate the viability of stored rice seeds cv. Chiang Phatthalung by a practical and efficient tetrazolium method. Seeds were stored at room temperature (28°C) and in cold storage (10°C) for 0, 3, 6, 9 and 12 months and then their viability and physiological quality was tested. Seeds of all treatments were soaked in water for 18 hours, cut longitudinally and immersed in 1% tetrazolium solution for two hours at 30°C according to the ISTA method, or in tetrazolium solution at different concentrations (0.0625, 0.125, 0.25 and 0.5%) and staining temperatures (30, 35, 40 and 45°C). The modified tetrazolium test with 0.125% tetrazolium concentration and staining at 40°C of stored rice seeds correlated positively with standard germination (r = 0.98, P < 0.01), soil seedling emergence (r = 0.98, P < 0.01), shoot length (r = 0.78, P < 0.01), root length (r = 0.81, P < 0.01) and seedling dry weight (r = 0.75, P < 0.01). Thus, this staining condition can be used to efficiently estimate the viability of freshly harvested and stored rice seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bacterial fruit blotch (BFB), caused by the seed-transmitted pathogen Acidovorax citrulli, poses a serious threat to cucurbitaceous crops worldwide. In this study, two biocontrol bacteria strains, Bacillus amyloliquefaciens (Ba-2) and Pseudomonas fluorescens (2P24), with significant antagonistic activity against A. citrulli were and applied by seed priming to control BFB seed transmission. Artificially infested watermelon and melon seeds were treated with the biocontrol strains by liquid or solid matrix seed priming. The seed bio-priming effects were evaluated under greenhouse conditions. Germination percentages were improved by seed priming treatments for melon, and seedling uniformity was higher for seeds primed with Ba-2 than for seeds from the other treatments for watermelon. Seedling disease incidence of untreated seeds were 6.5% for watermelon and 16.0% for melon, and water-priming resulted in similar disease progress curves as the untreated control. Seed priming with Ba-2 and 2P24 significantly reduced seedling BFB incidence for both watermelon and melon. Evaluation with naturally infested watermelon seeds primed with biocontrol strains indicated that 2P24 seed priming was more effective than Ba-2 (P < 0.05) and 2P24 solid matrix priming, reducing the seedling disease incidence to 1.3%. Seed bio-priming has potential as an effective and eco-friendly approach for suppressing bacterial fruit blotch seed-to-seedling transmission.
{"title":"Biocontrol of bacterial fruit blotch by seed priming with Bacillus amyloliquefaciens and Pseudomonas fluorescens","authors":"Yuxi Wang, Yanhong Qiu, Yu Lu, Xinyu Wang, Haijun Zhang, Ping Wu, Dexin Wang, Dianpeng Zhang, L. Luo, Xiu-lan Xu","doi":"10.15258/sst.2023.51.2.05","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.05","url":null,"abstract":"Bacterial fruit blotch (BFB), caused by the seed-transmitted pathogen Acidovorax citrulli, poses a serious threat to cucurbitaceous crops worldwide. In this study, two biocontrol bacteria strains, Bacillus amyloliquefaciens (Ba-2) and Pseudomonas fluorescens (2P24), with significant antagonistic activity against A. citrulli were and applied by seed priming to control BFB seed transmission. Artificially infested watermelon and melon seeds were treated with the biocontrol strains by liquid or solid matrix seed priming. The seed bio-priming effects were evaluated under greenhouse conditions. Germination percentages were improved by seed priming treatments for melon, and seedling uniformity was higher for seeds primed with Ba-2 than for seeds from the other treatments for watermelon. Seedling disease incidence of untreated seeds were 6.5% for watermelon and 16.0% for melon, and water-priming resulted in similar disease progress curves as the untreated control. Seed priming with Ba-2 and 2P24 significantly reduced seedling BFB incidence for both watermelon and melon. Evaluation with naturally infested watermelon seeds primed with biocontrol strains indicated that 2P24 seed priming was more effective than Ba-2 (P < 0.05) and 2P24 solid matrix priming, reducing the seedling disease incidence to 1.3%. Seed bio-priming has potential as an effective and eco-friendly approach for suppressing bacterial fruit blotch seed-to-seedling transmission.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.2.06
Nasrul Haq, S. Ilyas, M. R. Suhartanto, Y. Purwanto
This study aimed to examine the dormancy behaviour and effectiveness of various dormancy breaking treatments on cucumber seeds. Freshly harvested cucumber seeds of varieties CU-1047 and CU-1051 were extracted, dried and stored for eight weeks at ambient temperature. The stored seed lots were tested for seed germination once a week and hormone analysis (ABA, GA, zeatin and kinetin) every two weeks. In a second experiment, dormancy breaking methods using dry heat treatment (DHT) at 36, 50 or 80°C for various lengths of time. Chemical treatments were also tested, with a 24-hour soaking time using GA3, KNO3 or ultra-fine bubble (UFB) water. The results showed that the dormancy persistence in both varieties was three weeks after the seeds were harvested, by which time germination reached more than 85%. The decrease in ABA content during the storage period correlated with an increase in seed germination. The most effective methods of breaking dormancy were dry heat at 80°C for 24 hours (highest germination: 62%) and a DHT chamber (59%) compared with control (1%). An alternative method was soaking the seeds in 1% KNO3 followed by drying to the initial moisture content (55% germination). UFB water was not effective in breaking the dormancy of cucumber seeds.
{"title":"Dormancy behaviour and effectiveness of dormancy breaking methods in cucumber seeds (Cucumis sativus)","authors":"Nasrul Haq, S. Ilyas, M. R. Suhartanto, Y. Purwanto","doi":"10.15258/sst.2023.51.2.06","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.06","url":null,"abstract":"This study aimed to examine the dormancy behaviour and effectiveness of various dormancy breaking treatments on cucumber seeds. Freshly harvested cucumber seeds of varieties CU-1047 and CU-1051 were extracted, dried and stored for eight weeks at ambient temperature. The stored seed lots were tested for seed germination once a week and hormone analysis (ABA, GA, zeatin and kinetin) every two weeks. In a second experiment, dormancy breaking methods using dry heat treatment (DHT) at 36, 50 or 80°C for various lengths of time. Chemical treatments were also tested, with a 24-hour soaking time using GA3, KNO3 or ultra-fine bubble (UFB) water. The results showed that the dormancy persistence in both varieties was three weeks after the seeds were harvested, by which time germination reached more than 85%. The decrease in ABA content during the storage period correlated with an increase in seed germination. The most effective methods of breaking dormancy were dry heat at 80°C for 24 hours (highest germination: 62%) and a DHT chamber (59%) compared with control (1%). An alternative method was soaking the seeds in 1% KNO3 followed by drying to the initial moisture content (55% germination). UFB water was not effective in breaking the dormancy of cucumber seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article explored the effects of plasma pre-treatment on the germination of Platycodon grandiflorum seeds. By analysing the effects of plasma pre-treatment on the germination, seedling growth and development of P. grandiflorum seeds, the study explored the best technological parameters of plasma pre-treatment for P. grandiflorum production and application, and promoted the application of plasma technology in the seed pre-treatment of P. grandiflorum. Based on field experiments, this study verified that plasma pre-treatment can have positive biological effects on P. grandiflorum. The Boltzmann equation was used to fit the germination-time process curve and the logistic equation was used to fit the growth-time process curve. According to the fitted equations, the prediction of the germination stage and the true leaf stage of P. grandiflorum were realised. Plasma pre-technology can increase the field germination of P. grandiflorum seeds by 23.9%. Furthermore, the growth advantage of P. grandiflorum during the germination period is maintained through to the true leaf stage. Plasma pre-treatment significantly shortened the time for P. grandiflorum seedlings to reach the true leaf stage and promoted growth and development.
{"title":"Effects of plasma pre-treatment on field germination and seedling growth of Platycodon grandiflorum","authors":"Linjie Hao, Yong You, Yunting Hui, Lili Zhang, Changyong Shao, Decheng Wang, Tianyi Wang","doi":"10.15258/sst.2023.51.2.08","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.08","url":null,"abstract":"This article explored the effects of plasma pre-treatment on the germination of Platycodon grandiflorum seeds. By analysing the effects of plasma pre-treatment on the germination, seedling growth and development of P. grandiflorum seeds, the study explored the best technological parameters of plasma pre-treatment for P. grandiflorum production and application, and promoted the application of plasma technology in the seed pre-treatment of P. grandiflorum. Based on field experiments, this study verified that plasma pre-treatment can have positive biological effects on P. grandiflorum. The Boltzmann equation was used to fit the germination-time process curve and the logistic equation was used to fit the growth-time process curve. According to the fitted equations, the prediction of the germination stage and the true leaf stage of P. grandiflorum were realised. Plasma pre-technology can increase the field germination of P. grandiflorum seeds by 23.9%. Furthermore, the growth advantage of P. grandiflorum during the germination period is maintained through to the true leaf stage. Plasma pre-treatment significantly shortened the time for P. grandiflorum seedlings to reach the true leaf stage and promoted growth and development.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.2.11
F. R. Hay, Shabnam Rezaei, Dustin M. Wolkis, C. McGill
Moisture content determination is one of the most important and common assessments made on seeds. It contributes to the estimation of the value of a seed lot in commercial settings and the physiological status of seeds in research and conservation. The most common method for determining seed moisture content is by measuring the change in weight of a sample after drying at a constant temperature for a set period of time. This, however, renders the seeds unusable for other purposes, such as viability testing. Here, we review various methods for assessing the moisture status of seeds, including non-destructive methods.Controlling the amount of water in seeds is just as important as being able to measure the amount of water in seeds. Perhaps most notably, orthodox seeds are normally dried to low moisture content to maintain viability and vigour for longer, with the seeds either stored in breathable containers in a dry environment or sealed in air-tight containers such that they cannot reabsorb water. However, and especially in seed research, we are sometimes interested in having seeds at an intermediate or high moisture content. Hence, in this review we also discuss various ways to adjust the moisture content of seeds.
{"title":"Invited Review: Determination and control of seed moisture","authors":"F. R. Hay, Shabnam Rezaei, Dustin M. Wolkis, C. McGill","doi":"10.15258/sst.2023.51.2.11","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.11","url":null,"abstract":"Moisture content determination is one of the most important and common assessments made on seeds. It contributes to the estimation of the value of a seed lot in commercial settings and the physiological status of seeds in research and conservation. The most common method for determining seed moisture content is by measuring the change in weight of a sample after drying at a constant temperature for a set period of time. This, however, renders the seeds unusable for other purposes, such as viability testing. Here, we review various methods for assessing the moisture status of seeds, including non-destructive methods.Controlling the amount of water in seeds is just as important as being able to measure the amount of water in seeds. Perhaps most notably, orthodox seeds are normally dried to low moisture content to maintain viability and vigour for longer, with the seeds either stored in breathable containers in a dry environment or sealed in air-tight containers such that they cannot reabsorb water. However, and especially in seed research, we are sometimes interested in having seeds at an intermediate or high moisture content. Hence, in this review we also discuss various ways to adjust the moisture content of seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67092513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.3.09
M.H. Tian, A.J. Tang
Primula mallophylla and P. sertulum are wild ornamental species of south-west China, but the responses of their seeds to storage have not been determined. When seeds were dry-stored for 24 months at room temperature (RT) (ca. 21°C) and at 4°C, germination increased during the first six months, but then decreased over the following 18 months. The decrease in germination was slower for seeds stored at 4°C than for those stored at RT. When seeds of these two species were dry-stored at -20°C for 24 months, germination percentage was significantly higher than that of the control seeds by the sixth month; the high germination percentages persisted at 24 months. In addition, pre-germination treatments (i.e., immersion in 100 µmol L -1 GA 3 for 24 hours, cold stratification for 30 days or both) improved the final germination and reduced mean germination time of P. mallophylla seeds stored for 12 months at RT, but not those of P. sertulum . Taken together, storing seeds at -20°C is feasible and convenient for both primrose species; thus, it is possible to safely store seeds for future research and conservation.
报春花和毛毛报春花是西南地区的野生观赏植物，但其种子对贮藏的响应尚未确定。当种子在室温(约21℃)和室温(4℃)下干燥保存24个月时，发芽率在前6个月有所增加，但在随后的18个月有所下降。4℃贮藏的种子发芽率下降的速度比室温贮藏的慢。在-20℃干燥贮藏24个月后，到第6个月，发芽率显著高于对照种子;高发芽率持续到24个月。此外，萌发前处理(100微mol L -1 ga3浸泡24小时、冷分层30天或两者同时处理)提高了在室温下保存12个月的mallophylla种子的最终萌发率，并缩短了平均萌发时间，但对sertulum种子没有影响。综上所述，两种报春花在-20℃的条件下储存种子是可行且方便的;因此，可以安全地储存种子，以供将来的研究和保护。
{"title":"Effects of storage temperature and pre-treatments on seed germination of <i>Primula mallophylla</i> and <i>P. sertulum</i> (Primulaceae), two narrowly-distributed endemic species from southwest China","authors":"M.H. Tian, A.J. Tang","doi":"10.15258/sst.2023.51.3.09","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.09","url":null,"abstract":"Primula mallophylla and P. sertulum are wild ornamental species of south-west China, but the responses of their seeds to storage have not been determined. When seeds were dry-stored for 24 months at room temperature (RT) (ca. 21&deg;C) and at 4&deg;C, germination increased during the first six months, but then decreased over the following 18 months. The decrease in germination was slower for seeds stored at 4&deg;C than for those stored at RT. When seeds of these two species were dry-stored at -20&deg;C for 24 months, germination percentage was significantly higher than that of the control seeds by the sixth month; the high germination percentages persisted at 24 months. In addition, pre-germination treatments (i.e., immersion in 100 &micro;mol L -1 GA 3 for 24 hours, cold stratification for 30 days or both) improved the final germination and reduced mean germination time of P. mallophylla seeds stored for 12 months at RT, but not those of P. sertulum . Taken together, storing seeds at -20&deg;C is feasible and convenient for both primrose species; thus, it is possible to safely store seeds for future research and conservation.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135844384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.15258/sst.2023.51.3.07
Elham Abedi, Ramazan Ali Khavari-Nejad, Laleh Mosharraf, Sara Saadatmand, Hossein Ali Asadi-Gharneh
The aim of this research was to investigate the effectiveness of nano-selenium (nano-Se) on germination properties of vasha ( Dorema ammoniacum ), an endangered medicinal plant in Iran. The experiment was conducted in a completely randomised two-factor, experiment with five concentrations (0, 5, 10, 20, and 40 mg L -1 ) of nano-Se and seed prechilling treatment (4°C). All the germination characteristics, including percentage germination, mean germination and vigour indices were improved by nano-Se treatments ( p < 0.05). Among the treatments, the combination of prechilling and 20 mg L -1 nano-Se conditioning was the best treatment, both in improving germination and the growth characteristics of seedlings. It can be concluded that pretreatment of seeds with nano-Se and prechilling can be used for promoting germination of D. ammoniacum seeds.
本研究旨在研究纳米硒(nano-Se)对伊朗濒危药用植物vasha (Dorema amiacum)萌发特性的影响。实验采用完全随机双因素试验，采用5种浓度(0、5、10、20和40 mg L -1)的纳米硒和4℃的种子预冷处理。纳米硒处理提高了种子的发芽率、平均发芽率和活力指标(p <0.05)。其中，预冷与20 mg L -1纳米硒调质组合处理在提高种子萌发和幼苗生长特性方面效果最好。综上所述，纳米硒预处理和预冷处理可促进菊苣种子萌发。
{"title":"Effect of prechilling and nano-selenium on seed germination of <i>Dorema ammoniacum</i>","authors":"Elham Abedi, Ramazan Ali Khavari-Nejad, Laleh Mosharraf, Sara Saadatmand, Hossein Ali Asadi-Gharneh","doi":"10.15258/sst.2023.51.3.07","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.07","url":null,"abstract":"The aim of this research was to investigate the effectiveness of nano-selenium (nano-Se) on germination properties of vasha ( Dorema ammoniacum ), an endangered medicinal plant in Iran. The experiment was conducted in a completely randomised two-factor, experiment with five concentrations (0, 5, 10, 20, and 40 mg L -1 ) of nano-Se and seed prechilling treatment (4&deg;C). All the germination characteristics, including percentage germination, mean germination and vigour indices were improved by nano-Se treatments ( p &lt; 0.05). Among the treatments, the combination of prechilling and 20 mg L -1 nano-Se conditioning was the best treatment, both in improving germination and the growth characteristics of seedlings. It can be concluded that pretreatment of seeds with nano-Se and prechilling can be used for promoting germination of D. ammoniacum seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"239 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135403799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：