Yuan Yuan, Lei Fang, Mingqing Gu, Zhaoxu Lv, Changrong Lv, Nabi Ullah, Ahmad Umar, Shahid Hussain
ZnCo 2 O 4 nanosheets show excellent supercapacitive performance due to their well-developed and uniform architecture. The unique nanosheets with wide facets deliver outstanding electrochemical supercapacitor performance. As a result, the catalyst exhibits a specific capacitance of 700 F/g at a current density of 1 A/g. Interestingly, the material also shows outstanding cycling stability and loses only 2.75% even after 5000 cycles. The study confirms that ZnCo 2 O 4 based materials can be applied in high-energy as well as high-power density applications (energy storage, electric vehicles, and flexible electronic devices) due to their excellent morphology, wide facets, outstanding stability, and capacitance.
zno纳米片结构均匀、结构完善，具有优异的超级电容性能。独特的宽面纳米片提供了出色的电化学超级电容器性能。结果表明，该催化剂在电流密度为1 a /g时的比电容为700 F/g。有趣的是，该材料还表现出出色的循环稳定性，即使在5000次循环后也只损失2.75%。该研究证实，znco2o4基材料由于其优异的形貌、宽切面、出色的稳定性和电容，可以应用于高能量和高功率密度的应用(储能、电动汽车和柔性电子设备)。
{"title":"Wide Facets ZnCo<sub>2</sub>O<sub>4</sub> Nanosheets for High Electrochemical Supercapacitor Performances","authors":"Yuan Yuan, Lei Fang, Mingqing Gu, Zhaoxu Lv, Changrong Lv, Nabi Ullah, Ahmad Umar, Shahid Hussain","doi":"10.1166/sam.2023.4529","DOIUrl":"https://doi.org/10.1166/sam.2023.4529","url":null,"abstract":"ZnCo 2 O 4 nanosheets show excellent supercapacitive performance due to their well-developed and uniform architecture. The unique nanosheets with wide facets deliver outstanding electrochemical supercapacitor performance. As a result, the catalyst exhibits a specific capacitance of 700 F/g at a current density of 1 A/g. Interestingly, the material also shows outstanding cycling stability and loses only 2.75% even after 5000 cycles. The study confirms that ZnCo 2 O 4 based materials can be applied in high-energy as well as high-power density applications (energy storage, electric vehicles, and flexible electronic devices) due to their excellent morphology, wide facets, outstanding stability, and capacitance.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this research was to demonstrate the impact of ursolic acid (UA) in Prunella vulgaris extracts on the proliferation of papillary thyroid carcinoma (PTC) cells through the p53MAPK signaling. Effects of Prunella vulgaris extracts on TPC-1 cell proliferation were analyzed by intervening with various concentrations of UA, including negative control (NC) group, solvent control (SC) group, 3 μ M UA group, 6 μ M UA group, 12 μ M UA group, and 15 μ M UA group. Flow cytometry was adopted to evaluate apoptosis in TPC-1 cells, while real-time fluorescent quantitative (RT-q) PCR was implemented to assess expression (EP) of Bax and Bcl-2 in TPC-1 cells following UA intervention. RT-qPCR and Western blotting were employed to examine the differential EP levels of cell apoptosis, Bax, and Bcl-2 proteins. RT-qPCR was utilized to investigate the influence of UA on EP of various genes in MAPK pathway. The ethyl acetate extract exhibited the most notable inhibitory effect on TPC-1 cells. The content of UA in Prunella vulgaris increased gradually with the extension of ultrasonic time. The growth curve of TPC-1 cells demonstrated an initial increase followed by a decrease with increasing time. As the concentration increased, cell proportion in S phase increased, while the proportions in the GO-G1 and G2-M phases decreased, indicating that UA concentration-dependently arrested cells in the S phase. The level of Bax mRNA exhibited an increasing trend with increasing concentration, and the 12 μ M UA and 15 μ M UA groups demonstrated remarkable differences versus NC group ( P <0.01). Bcl-2 protein demonstrated a decreasing trend with increasing concentration, and the 6 μ M UA, 12 μ M UA, and 15 μ M UA groups exhibited considerable differences relative to NC group ( P < 0.05). Additionally, pro-apoptotic protein Bax increased, while that of anti-apoptotic protein Bcl-2 decreased. UA treatment upregulated EP of the p53 gene in the MAPK pathway. Genes such as ERK, MEK, TSHR, Ras, p53, BRAF, PAK4, and PAKCa were downregulated. In summary, UA can upregulate EP of the p53 gene in the MAPK pathway, greatly inhibit proliferation of TPC-1 cells in PTC, and promote apoptosis. These findings provide insights for therapy of thyroid cancer.
本研究的目的是通过p53MAPK信号通路证明夏枯草提取物中熊果酸(UA)对甲状腺乳头状癌(PTC)细胞增殖的影响。通过不同浓度的UA干预，包括阴性对照(NC)组、溶剂对照(SC)组、3 μ M UA组、6 μ M UA组、12 μ M UA组和15 μ M UA组，分析夏枯草提取物对TPC-1细胞增殖的影响。采用流式细胞术检测TPC-1细胞凋亡情况，实时荧光定量PCR检测UA干预后TPC-1细胞中Bax和Bcl-2的表达情况。采用RT-qPCR和Western blotting检测EP对细胞凋亡、Bax和Bcl-2蛋白的影响。采用RT-qPCR方法研究UA对MAPK通路中各基因EP的影响。乙酸乙酯提取物对TPC-1细胞的抑制作用最显著。随着超声时间的延长，夏枯草中UA的含量逐渐升高。随着时间的增加，TPC-1细胞的生长曲线呈现先升高后降低的趋势。随着浓度的增加，处于S期的细胞比例增加，而处于GO-G1期和G2-M期的细胞比例减少，表明UA浓度依赖性阻滞细胞处于S期。Bax mRNA水平随浓度的增加呈升高趋势，12 μ M UA组和15 μ M UA组与NC组相比差异显著(P <0.01)。Bcl-2蛋白随浓度的增加呈下降趋势，6 μ M UA、12 μ M UA和15 μ M UA组与NC组相比差异显著(P <0.05)。促凋亡蛋白Bax升高，抗凋亡蛋白Bcl-2降低。UA治疗上调了MAPK通路中p53基因的EP。ERK、MEK、TSHR、Ras、p53、BRAF、PAK4、PAKCa等基因下调。综上所述，UA可以上调MAPK通路中p53基因的EP，显著抑制PTC中TPC-1细胞的增殖，促进细胞凋亡。这些发现为甲状腺癌的治疗提供了新的思路。
{"title":"Effects of Ursolic Acid in Extracts of <i>Prunella vulgaris</i> on the Proliferation of Thyroid Papillary Carcinoma Cells Under the p53MAPK Signaling Pathway","authors":"Chao Hu, Qi Zhang, Dongxian Liu, Chao Li, Ming Li","doi":"10.1166/sam.2023.4520","DOIUrl":"https://doi.org/10.1166/sam.2023.4520","url":null,"abstract":"The aim of this research was to demonstrate the impact of ursolic acid (UA) in Prunella vulgaris extracts on the proliferation of papillary thyroid carcinoma (PTC) cells through the p53MAPK signaling. Effects of Prunella vulgaris extracts on TPC-1 cell proliferation were analyzed by intervening with various concentrations of UA, including negative control (NC) group, solvent control (SC) group, 3 μ M UA group, 6 μ M UA group, 12 μ M UA group, and 15 μ M UA group. Flow cytometry was adopted to evaluate apoptosis in TPC-1 cells, while real-time fluorescent quantitative (RT-q) PCR was implemented to assess expression (EP) of Bax and Bcl-2 in TPC-1 cells following UA intervention. RT-qPCR and Western blotting were employed to examine the differential EP levels of cell apoptosis, Bax, and Bcl-2 proteins. RT-qPCR was utilized to investigate the influence of UA on EP of various genes in MAPK pathway. The ethyl acetate extract exhibited the most notable inhibitory effect on TPC-1 cells. The content of UA in Prunella vulgaris increased gradually with the extension of ultrasonic time. The growth curve of TPC-1 cells demonstrated an initial increase followed by a decrease with increasing time. As the concentration increased, cell proportion in S phase increased, while the proportions in the GO-G1 and G2-M phases decreased, indicating that UA concentration-dependently arrested cells in the S phase. The level of Bax mRNA exhibited an increasing trend with increasing concentration, and the 12 μ M UA and 15 μ M UA groups demonstrated remarkable differences versus NC group ( P <0.01). Bcl-2 protein demonstrated a decreasing trend with increasing concentration, and the 6 μ M UA, 12 μ M UA, and 15 μ M UA groups exhibited considerable differences relative to NC group ( P < 0.05). Additionally, pro-apoptotic protein Bax increased, while that of anti-apoptotic protein Bcl-2 decreased. UA treatment upregulated EP of the p53 gene in the MAPK pathway. Genes such as ERK, MEK, TSHR, Ras, p53, BRAF, PAK4, and PAKCa were downregulated. In summary, UA can upregulate EP of the p53 gene in the MAPK pathway, greatly inhibit proliferation of TPC-1 cells in PTC, and promote apoptosis. These findings provide insights for therapy of thyroid cancer.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigated the prognostic value of telomere-related genes in oral squamous cell carcinoma (OSCC) using the TCGA-OSCC dataset and GSE41613 external validation set. We identified differentially expressed genes (DEGs) between OSCC and control samples and intersected them with telomere-related genes. Three risk model genes (IGF2BP2, EIF5A2, and PLOD2) were obtained through Cox and LASSO analyses. A risk model was constructed based on the expression of these genes. The OSCC samples were divided into high and low-risk groups using the median risk score. Univariate and multivariate Cox analyses identified risk score and age as independent prognostic factors. Gene set enrichment analysis revealed enrichment in chemical stimulus and appendage development pathways. We constructed a transcription factor (TF)-mRNA network involving two mRNAs (EIF5A2, PLOD2) and 17 TFs, including STAT1-EIF5A2 and TEAD1-EIF5A2. Immune-infiltration analysis showed significant differences in the abundance of 11 immune cells between the high and low-risk groups, including T cells CD8, activated mast cells, and macrophages M0. Our findings contribute to the development of a telomere-related risk model (including IGF2BP2, EIF5A2, and PLOD2) for predicting the prognosis of OSCC, providing new insights for further studies in this area.
{"title":"Construction of a New Prognostic Model for Oral Squamous Cell Carcinoma Based on Telomere-Related Genes","authors":"Lin Liu, Jia Liu, Keyi Wang, Yuchi Zhu","doi":"10.1166/sam.2023.4532","DOIUrl":"https://doi.org/10.1166/sam.2023.4532","url":null,"abstract":"We investigated the prognostic value of telomere-related genes in oral squamous cell carcinoma (OSCC) using the TCGA-OSCC dataset and GSE41613 external validation set. We identified differentially expressed genes (DEGs) between OSCC and control samples and intersected them with telomere-related genes. Three risk model genes (IGF2BP2, EIF5A2, and PLOD2) were obtained through Cox and LASSO analyses. A risk model was constructed based on the expression of these genes. The OSCC samples were divided into high and low-risk groups using the median risk score. Univariate and multivariate Cox analyses identified risk score and age as independent prognostic factors. Gene set enrichment analysis revealed enrichment in chemical stimulus and appendage development pathways. We constructed a transcription factor (TF)-mRNA network involving two mRNAs (EIF5A2, PLOD2) and 17 TFs, including STAT1-EIF5A2 and TEAD1-EIF5A2. Immune-infiltration analysis showed significant differences in the abundance of 11 immune cells between the high and low-risk groups, including T cells CD8, activated mast cells, and macrophages M0. Our findings contribute to the development of a telomere-related risk model (including IGF2BP2, EIF5A2, and PLOD2) for predicting the prognosis of OSCC, providing new insights for further studies in this area.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ran Hu, Hongliang Guo, Bing Li, Zhifeng Xu, Weichao Zhang
The cable is subjected to a variety of influences such as electricity and heat, which may cause damage to the dielectric strength and cause irreversible effects. In this paper, the thermal aging performance of 135 °C and 175 °C was studied by studying the cross-linked polyethylene, carbon black shield, conductive silicone rubber and insulating silicone rubber in the cable body and joint, and the brittle section of the material was observed by scanning electron microscopy. Infrared spectroscopy was used to analyze the absorption peak of the observation material; The crystallization properties of materials were observed by differential scanning calorimetry. Alternating Current breakdown test, broadband dielectric spectrum test and volume resistivity test were used to observe the electrical properties of the material; Tensile experiments were used to observe the mechanical physics of materials.
{"title":"Thermal Aged Parameters Characteristics of Cables Crosslinked Polyethylene and Accessories Silicone Rubber Materials","authors":"Ran Hu, Hongliang Guo, Bing Li, Zhifeng Xu, Weichao Zhang","doi":"10.1166/sam.2023.4528","DOIUrl":"https://doi.org/10.1166/sam.2023.4528","url":null,"abstract":"The cable is subjected to a variety of influences such as electricity and heat, which may cause damage to the dielectric strength and cause irreversible effects. In this paper, the thermal aging performance of 135 °C and 175 °C was studied by studying the cross-linked polyethylene, carbon black shield, conductive silicone rubber and insulating silicone rubber in the cable body and joint, and the brittle section of the material was observed by scanning electron microscopy. Infrared spectroscopy was used to analyze the absorption peak of the observation material; The crystallization properties of materials were observed by differential scanning calorimetry. Alternating Current breakdown test, broadband dielectric spectrum test and volume resistivity test were used to observe the electrical properties of the material; Tensile experiments were used to observe the mechanical physics of materials.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaodong Wang, Chunyan Yu, Wei Ni, Haolong Su, Jiahao Tan
To investigate the potential utilization of materials exhibiting negative thermal expansion in snow melting and ice suppression applications, an orthogonal test was conducted to ascertain the optimal ratio of effective constituents in said materials. The thermal expansion coefficient of the porous carrier for negative thermal expansion materials was examined, and modifications were made to the material used for snow melting and ice suppression. In conclusion, the researchers successfully developed snow melting and ice suppression materials with low thermal expansion that met the desired performance criteria. Furthermore, they conducted an analysis of the precipitation rate, as well as the snow melting and ice suppression effectiveness of these materials across various temperature ranges. According to the results, the thermal expansion coefficient of the carrier Z1 is positive and varies slightly in the temperature range of −30 °C~−10 °C. In the temperature range of −10 °C~0 °C, the thermal expansion coefficient is negative, and it has negative thermal expansion characteristics. At 0 °C~60 °C, its thermal expansion coefficient first increases and then decreases. In the low temperature stage, the amount of salt precipitation decreases with the decrease of temperature. In the conductivity test, the conductivity at 0 °C is greater than that at −5 °C, and much greater than that at 10 °C, the presence of a negative thermal expansion coefficient has been observed to exert a specific influence on the precipitation of materials used for snow melting and ice suppression. In addition, with the increase of temperature, the amount of salt precipitation increases. Whereas, when the temperature is higher than 35 °C, due to the thermal expansion characteristics of the carrier, the expansion of the volume of snow melting and ice suppression materials is comparatively limited, which results in a not greatly increased precipitation rate of the snow melting and ice suppression components compared with that at 35 °C.
{"title":"Preparation and Properties of Negative Thermal Expansion Snow Melting and Ice Suppression Materials","authors":"Xiaodong Wang, Chunyan Yu, Wei Ni, Haolong Su, Jiahao Tan","doi":"10.1166/sam.2023.4564","DOIUrl":"https://doi.org/10.1166/sam.2023.4564","url":null,"abstract":"To investigate the potential utilization of materials exhibiting negative thermal expansion in snow melting and ice suppression applications, an orthogonal test was conducted to ascertain the optimal ratio of effective constituents in said materials. The thermal expansion coefficient of the porous carrier for negative thermal expansion materials was examined, and modifications were made to the material used for snow melting and ice suppression. In conclusion, the researchers successfully developed snow melting and ice suppression materials with low thermal expansion that met the desired performance criteria. Furthermore, they conducted an analysis of the precipitation rate, as well as the snow melting and ice suppression effectiveness of these materials across various temperature ranges. According to the results, the thermal expansion coefficient of the carrier Z1 is positive and varies slightly in the temperature range of −30 °C~−10 °C. In the temperature range of −10 °C~0 °C, the thermal expansion coefficient is negative, and it has negative thermal expansion characteristics. At 0 °C~60 °C, its thermal expansion coefficient first increases and then decreases. In the low temperature stage, the amount of salt precipitation decreases with the decrease of temperature. In the conductivity test, the conductivity at 0 °C is greater than that at −5 °C, and much greater than that at 10 °C, the presence of a negative thermal expansion coefficient has been observed to exert a specific influence on the precipitation of materials used for snow melting and ice suppression. In addition, with the increase of temperature, the amount of salt precipitation increases. Whereas, when the temperature is higher than 35 °C, due to the thermal expansion characteristics of the carrier, the expansion of the volume of snow melting and ice suppression materials is comparatively limited, which results in a not greatly increased precipitation rate of the snow melting and ice suppression components compared with that at 35 °C.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bin Xie, Jun-Jie He, Lei Zhao, Yu-Chen Sun, Sen-Lin Li, Jing Li
Supercapacitors have emerged as one of the devices most likely to facilitate major breakthroughs in energy storage technology. With the advantages of wide source of raw materials, good electrical conductivity and easy preparation, biomass activated carbon has become the most promising materials to promote for application of supercapacitors. Biomass activated carbon still has problems such as low energy density and unenvironmentally preparation process. This work summarizes the relevant research about the manufacture of biomass activated carbon for supercapacitors in recent years, including the consistency between theoretical performance and experimental data, and the correlation between preparation technology and basic characteristics. Furthermore, the specific range of basic characteristics for high-performance biomass activated carbon was pointed out, which were pore size distribution, conductivity, specific surface area and surface heteroatom doping. The most suitable manufacturing process for high performance biomass activated carbon was demonstrated. This significance was important for promoting the large-scale production of biomass activated carbon for Supercapacitors.
{"title":"Biomass Activated Carbon: The Electrode Material to Promote the Large-Scale Production of Supercapacitors","authors":"Bin Xie, Jun-Jie He, Lei Zhao, Yu-Chen Sun, Sen-Lin Li, Jing Li","doi":"10.1166/sam.2023.4554","DOIUrl":"https://doi.org/10.1166/sam.2023.4554","url":null,"abstract":"Supercapacitors have emerged as one of the devices most likely to facilitate major breakthroughs in energy storage technology. With the advantages of wide source of raw materials, good electrical conductivity and easy preparation, biomass activated carbon has become the most promising\u0000 materials to promote for application of supercapacitors. Biomass activated carbon still has problems such as low energy density and unenvironmentally preparation process. This work summarizes the relevant research about the manufacture of biomass activated carbon for supercapacitors in recent\u0000 years, including the consistency between theoretical performance and experimental data, and the correlation between preparation technology and basic characteristics. Furthermore, the specific range of basic characteristics for high-performance biomass activated carbon was pointed out, which\u0000 were pore size distribution, conductivity, specific surface area and surface heteroatom doping. The most suitable manufacturing process for high performance biomass activated carbon was demonstrated. This significance was important for promoting the large-scale production of biomass activated\u0000 carbon for Supercapacitors.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hadil Faris Alotaibi, El-Sayed Khafagy, Mohammad Y. Alfaifi, Jawaher Abdullah Alamoudi, Samar Zuhair Alshawwa, Rana Saeed Alqahtani, Sarah Salem Alamrani, Amr Selim Abu Lila
Medication repurposing is one of the effective strategies in approving drugs for new therapeutic indications. Clarithromycin is a well-known macrolide antibiotic that is commonly used for the treatment of a wide variety of bacterial infections. However, extensive preclinical and clinical studies have recently revealed the efficacy of clarithromycin in treating various tumors in combination with conventional therapy. In this study, we formulated a clarithromycin nanoemulsion using pumpkin seed oil as a natural nano-carrier to study the possible cytotoxic effects against colorectal (HCT 116), breast (MCF-7), and liver cancer (HepG2) in vitro . The formulated nanoemulsion was characterized for droplet size distribution, surface charge, drug loading and in vitro drug release. The droplet size of clarithromycin nanoemulsion was 360.6±42.5 nm and zeta potential was −31.6± 2.8 mV. Incorporating the lipophilic drug, clarithromycin, within nanoemulsion significantly heightened in vitro drug release, compared to plain drug. The anticancer properties of the nanoemulsion formulation were examined using sulforhodamine B (SRB) assay as well as cell cycle and apoptosis analyses. Clarithromycin-loaded pumpkin seed oil-based nanoemulsion remarkably diminished the viability of all tested cell lines, compared to either plain clarithromycin or plank pumpkin seed oil nanoemulsion. In addition, incorporating clarithromycin within pumpkin seed oil-based nanoemulsion synergistically augmented the cytotoxic efficacy of clarithromycin against various cancer cell lines via advocating considerable cell cycle arrest with subsequent elicitation of potent apoptotic response. These results support the potential use of clarithromycin in cancer therapy, and merit future translational research.
{"title":"Cytotoxic Potential of Clarithromycin-Loaded Pumpkin Seed Oil-Based Nanoemulsion on Human Breast, Hepatic and Colorectal Cancer Cells","authors":"Hadil Faris Alotaibi, El-Sayed Khafagy, Mohammad Y. Alfaifi, Jawaher Abdullah Alamoudi, Samar Zuhair Alshawwa, Rana Saeed Alqahtani, Sarah Salem Alamrani, Amr Selim Abu Lila","doi":"10.1166/sam.2023.4523","DOIUrl":"https://doi.org/10.1166/sam.2023.4523","url":null,"abstract":"Medication repurposing is one of the effective strategies in approving drugs for new therapeutic indications. Clarithromycin is a well-known macrolide antibiotic that is commonly used for the treatment of a wide variety of bacterial infections. However, extensive preclinical and clinical studies have recently revealed the efficacy of clarithromycin in treating various tumors in combination with conventional therapy. In this study, we formulated a clarithromycin nanoemulsion using pumpkin seed oil as a natural nano-carrier to study the possible cytotoxic effects against colorectal (HCT 116), breast (MCF-7), and liver cancer (HepG2) in vitro . The formulated nanoemulsion was characterized for droplet size distribution, surface charge, drug loading and in vitro drug release. The droplet size of clarithromycin nanoemulsion was 360.6±42.5 nm and zeta potential was −31.6± 2.8 mV. Incorporating the lipophilic drug, clarithromycin, within nanoemulsion significantly heightened in vitro drug release, compared to plain drug. The anticancer properties of the nanoemulsion formulation were examined using sulforhodamine B (SRB) assay as well as cell cycle and apoptosis analyses. Clarithromycin-loaded pumpkin seed oil-based nanoemulsion remarkably diminished the viability of all tested cell lines, compared to either plain clarithromycin or plank pumpkin seed oil nanoemulsion. In addition, incorporating clarithromycin within pumpkin seed oil-based nanoemulsion synergistically augmented the cytotoxic efficacy of clarithromycin against various cancer cell lines via advocating considerable cell cycle arrest with subsequent elicitation of potent apoptotic response. These results support the potential use of clarithromycin in cancer therapy, and merit future translational research.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"2 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper, metal–organic frameworks (MOFs) are applied to get rid of Congo red from effluent in an inexpensive and effective manner. Co-containing MOFs (Co-MOF) were prepared via solvothermal treatment, and their structures were tested by performing SEM, TG, and XRD. The MOFs were studied for the removal of CR from wastewater, and the kinetics of the adsorption process were investigated. The results indicated that the pseudo second-order kinetic model properly describes the removal of CR and the maximum adsorption capacity within 5 h is 355.2 mg/g for Co-MOF. The adsorption capacity of the MOFs was evaluated under different pH conditions. This work highlights the broad application prospect of MOFs in sewage disposal.
{"title":"Synthesis of Metal–Organic Frameworks for the Adsorption of Congo Red from Wastewater","authors":"Qinhui Ren, Peng Chen, Yaqi Yang, Fuhua Wei, Hongliang Chen, Siyuan Wang, Zhao Liang","doi":"10.1166/sam.2023.4563","DOIUrl":"https://doi.org/10.1166/sam.2023.4563","url":null,"abstract":"In this paper, metal–organic frameworks (MOFs) are applied to get rid of Congo red from effluent in an inexpensive and effective manner. Co-containing MOFs (Co-MOF) were prepared via solvothermal treatment, and their structures were tested by performing SEM, TG, and XRD. The MOFs were studied for the removal of CR from wastewater, and the kinetics of the adsorption process were investigated. The results indicated that the pseudo second-order kinetic model properly describes the removal of CR and the maximum adsorption capacity within 5 h is 355.2 mg/g for Co-MOF. The adsorption capacity of the MOFs was evaluated under different pH conditions. This work highlights the broad application prospect of MOFs in sewage disposal.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jian Qin, Jie Zeng, Yuetong Ning, Shenglin Wen, Wen Zeng
In this paper, a series of calcium silicate thermal insulation materials were prepared by hydrothermal method, and their structure, mechanical properties and thermal insulation properties were systematically studied by adjusting their preparation process, aiming to develop a new type of calcium silicate thermal insulation material with excellent performance. We found that the product gradually changed from a dense flocculent substance on the surface to a loose needle rod substance, and the grain grew, and the crystallinity increased with the increase of synthesis temperature. As the initial calcium silicon ratio increases, the surface pores of the product increase, and the surface gradually becomes loose from dense, resulting in the appearance of tobermorite and xonotlite phases.
{"title":"Preparation and Performance of Building Fire Protection and Insulation Materials","authors":"Jian Qin, Jie Zeng, Yuetong Ning, Shenglin Wen, Wen Zeng","doi":"10.1166/sam.2023.4527","DOIUrl":"https://doi.org/10.1166/sam.2023.4527","url":null,"abstract":"In this paper, a series of calcium silicate thermal insulation materials were prepared by hydrothermal method, and their structure, mechanical properties and thermal insulation properties were systematically studied by adjusting their preparation process, aiming to develop a new type of calcium silicate thermal insulation material with excellent performance. We found that the product gradually changed from a dense flocculent substance on the surface to a loose needle rod substance, and the grain grew, and the crystallinity increased with the increase of synthesis temperature. As the initial calcium silicon ratio increases, the surface pores of the product increase, and the surface gradually becomes loose from dense, resulting in the appearance of tobermorite and xonotlite phases.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study employed network pharmacology to investigate how Linggui Zhugan Decoction (LGZGD) may treat obesity. The TCMSP platform was used to select active ingredients and targets of LGZGD based on drug ADME properties, forming a component-target network. Obesity-related targets were identified from various databases, and a global network was constructed to analyze interactions between components, targets, and disease-related proteins. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted using G: profiler. Molecular docking using AutoDockTools validated the binding of key active components to core targets. The study identified 120 active ingredients and 201 targets for LGZGD, with 84 targets related to obesity. GO analysis revealed various biological processes, cellular components, and molecular functions associated with obesity, including lipid response and cellular response to chemical stimuli. KEGG pathway analysis highlighted signaling pathways such as AGE-RAGE signaling in diabetic complications, cancer pathways, IL-17 signaling, and neuroactive ligand-receptor interaction signaling. Molecular docking confirmed that the core active components of LGZGD tightly bind to key targets involved in obesity treatment. This study provides a preliminary understanding of the pharmacological basis and efficacy mechanism of LGZGD in treating obesity, supporting its traditional use and offering a theoretical foundation for further research.
{"title":"Mechanisms of Linggui Zhugan Decoction in the Treatment of Obesity Based on Network Pharmacology","authors":"Chunmei Liu, Li Zhang, Yubin Yang","doi":"10.1166/sam.2023.4514","DOIUrl":"https://doi.org/10.1166/sam.2023.4514","url":null,"abstract":"This study employed network pharmacology to investigate how Linggui Zhugan Decoction (LGZGD) may treat obesity. The TCMSP platform was used to select active ingredients and targets of LGZGD based on drug ADME properties, forming a component-target network. Obesity-related targets were identified from various databases, and a global network was constructed to analyze interactions between components, targets, and disease-related proteins. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted using G: profiler. Molecular docking using AutoDockTools validated the binding of key active components to core targets. The study identified 120 active ingredients and 201 targets for LGZGD, with 84 targets related to obesity. GO analysis revealed various biological processes, cellular components, and molecular functions associated with obesity, including lipid response and cellular response to chemical stimuli. KEGG pathway analysis highlighted signaling pathways such as AGE-RAGE signaling in diabetic complications, cancer pathways, IL-17 signaling, and neuroactive ligand-receptor interaction signaling. Molecular docking confirmed that the core active components of LGZGD tightly bind to key targets involved in obesity treatment. This study provides a preliminary understanding of the pharmacological basis and efficacy mechanism of LGZGD in treating obesity, supporting its traditional use and offering a theoretical foundation for further research.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135736321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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