C. Ai, Zhi Xu, Yujue Wang, Baolei Huang, Jiandong Tai
Colorectal carcinoma (CRC) is a common tumor in the digestive system. This study aims to elucidate the possible relationship between abnormally expressed HOXD9 and the malignant process of CRC. HOXD9 levels were analyzed in CRC and adjacent non-tumoral tissues to evaluate its prognostic value in CRC patients. Knockdown of HOXD9 was performed, and the proliferative and migratory capacities of LoVo and LS513 cells were assessed using CCK-8, transwell, and wound healing assays. Bioinformatic analysis and dual-luciferase reporter assay revealed the interaction between HOXD9 and KLK9. Rescue experiments were conducted to elucidate the co-regulation of HOXD9 and KLK9 on CRC cell behaviors. HOXD9 was upregulated in CRC tissues, and high level of HOXD9 predicted poor prognosis in CRC patients. HOXD9 was identically upregulated in CRC cell lines, especially LoVo and LS513 cells, which were used for generating HOXD9 knockdown models by transfection of sh-HOXD9. Knockdown of HOXD9 weakened proliferative and migratory capacities in CRC cells. KLK9 was the target binding HOXD9, which was downregulated in CRC tissues and cell lines. Knockdown of KLK9 reversed the inhibited proliferative and migratory capacities in CRC cells owing to HOXD9 knockdown. Highly expressed HOXD9 in CRC tissues is closely linked to the prognosis. HOXD9 stimulates CRC cells to proliferate and migrate by upregulating KLK9.
{"title":"HOXD9 Stimulates the Process of Colorectal Carcinoma by Regulating KLK9","authors":"C. Ai, Zhi Xu, Yujue Wang, Baolei Huang, Jiandong Tai","doi":"10.1166/sam.2023.4515","DOIUrl":"https://doi.org/10.1166/sam.2023.4515","url":null,"abstract":"Colorectal carcinoma (CRC) is a common tumor in the digestive system. This study aims to elucidate the possible relationship between abnormally expressed HOXD9 and the malignant process of CRC. HOXD9 levels were analyzed in CRC and adjacent non-tumoral tissues to evaluate its prognostic\u0000 value in CRC patients. Knockdown of HOXD9 was performed, and the proliferative and migratory capacities of LoVo and LS513 cells were assessed using CCK-8, transwell, and wound healing assays. Bioinformatic analysis and dual-luciferase reporter assay revealed the interaction between HOXD9 and\u0000 KLK9. Rescue experiments were conducted to elucidate the co-regulation of HOXD9 and KLK9 on CRC cell behaviors. HOXD9 was upregulated in CRC tissues, and high level of HOXD9 predicted poor prognosis in CRC patients. HOXD9 was identically upregulated in CRC cell lines, especially LoVo and LS513\u0000 cells, which were used for generating HOXD9 knockdown models by transfection of sh-HOXD9. Knockdown of HOXD9 weakened proliferative and migratory capacities in CRC cells. KLK9 was the target binding HOXD9, which was downregulated in CRC tissues and cell lines. Knockdown of KLK9 reversed the\u0000 inhibited proliferative and migratory capacities in CRC cells owing to HOXD9 knockdown. Highly expressed HOXD9 in CRC tissues is closely linked to the prognosis. HOXD9 stimulates CRC cells to proliferate and migrate by upregulating KLK9.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49184669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Amin, Hanif Ullah, Kaffayatullah Khan, Mudassir Iqbal
In the current study, the efficiency of the carbon-fiber reinforced polymer (CFRP) strengthening technique is experimentally applied to reinforced concrete (RC) beams. In total, four types of test beam specimens are fabricated and tested, labeled as B-1, B-2, B-3, and B-4 for controlled specimens; the beams strengthened at high-level bending-moment regions, beams strengthened at bending-moment regions with enhanced development length, and U-wrapped shear plus flexural strengthened beams, respectively. The control specimens were fabricated under-reinforced in order to exhibit ductile failure according to ACI 318-08. The variation in the CFRP external configuration included a change in the bond length and use of U-shaped strips to limit the CFRP debonding. For strengthening the beams, Sika group CFRP was used in this study. The test specimens were subjected to three-point loading following seven days of strengthening. The test result includes failure modes, load displacement curves, and stiffness degradation. The proposed CFRP strengthening configuration of the RC beams (B-2, B-3, and B-4) demonstrated improved behavior of their load response as compared to that of control beam (B-1). The CFRP configuration used of RC beam B-4 exhibited almost double flexural strength to that of control beam B-1. Moreover, the deformability of the CFRP strengthened RC beams was also increased.
{"title":"Load-Displacement and Stiffness Characteristics of Carbon-Fiber Reinforced Polymer (CFRP) Strengthened Reinforced Concrete Beams","authors":"M. Amin, Hanif Ullah, Kaffayatullah Khan, Mudassir Iqbal","doi":"10.1166/sam.2023.4468","DOIUrl":"https://doi.org/10.1166/sam.2023.4468","url":null,"abstract":"In the current study, the efficiency of the carbon-fiber reinforced polymer (CFRP) strengthening technique is experimentally applied to reinforced concrete (RC) beams. In total, four types of test beam specimens are fabricated and tested, labeled as B-1, B-2, B-3, and B-4 for controlled\u0000 specimens; the beams strengthened at high-level bending-moment regions, beams strengthened at bending-moment regions with enhanced development length, and U-wrapped shear plus flexural strengthened beams, respectively. The control specimens were fabricated under-reinforced in order\u0000 to exhibit ductile failure according to ACI 318-08. The variation in the CFRP external configuration included a change in the bond length and use of U-shaped strips to limit the CFRP debonding. For strengthening the beams, Sika group CFRP was used in this study. The test specimens were\u0000 subjected to three-point loading following seven days of strengthening. The test result includes failure modes, load displacement curves, and stiffness degradation. The proposed CFRP strengthening configuration of the RC beams (B-2, B-3, and B-4) demonstrated improved behavior of their load\u0000 response as compared to that of control beam (B-1). The CFRP configuration used of RC beam B-4 exhibited almost double flexural strength to that of control beam B-1. Moreover, the deformability of the CFRP strengthened RC beams was also increased.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46529524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Exploring an efficient and photostable heterojunction photocatalyst is a pivotal scientific topic for global energy and environmental concerns. Herein, we demonstrated an efficient and facile strategy to synthesize of Bi2MoO6/g-C3N4 heterojunctioned nanosheets (denoted as BM/CNs hereafter). The photocatalytic degradation of Rh B as a model pollutant indicated that the optimized 40%-BM/CNs catalyst could degrade 96.3% Rh B solution within 120 min. The degradation efficiency was 4.2 times and 6.8 times higher than that of pure Bi2MoO6 and pristine g-C3N4, respectively. The enhanced photocatalytic performance of 40%-BM/CNs catalyst might be mainly attributed to the construction of Z-scheme heterostructure, which effectively improved the efficiency of electron–hole separation. This work proposed a possible Z-scheme photocatalytic mechanism to elucidate the enhanced photochemical properties.
{"title":"Facile Fabrication of Bi2MoO6/g-C3N4 Heterojunction Nanosheets: Facile Synthesis and Enhanced Visible Light Photocatalytic Property","authors":"Fu Su, Jian-Hua Huang, Yanhua Xu","doi":"10.1166/sam.2023.4496","DOIUrl":"https://doi.org/10.1166/sam.2023.4496","url":null,"abstract":"Exploring an efficient and photostable heterojunction photocatalyst is a pivotal scientific topic for global energy and environmental concerns. Herein, we demonstrated an efficient and facile strategy to synthesize of Bi2MoO6/g-C3N4 heterojunctioned\u0000 nanosheets (denoted as BM/CNs hereafter). The photocatalytic degradation of Rh B as a model pollutant indicated that the optimized 40%-BM/CNs catalyst could degrade 96.3% Rh B solution within 120 min. The degradation efficiency was 4.2 times and 6.8 times higher than that of pure Bi2MoO6\u0000 and pristine g-C3N4, respectively. The enhanced photocatalytic performance of 40%-BM/CNs catalyst might be mainly attributed to the construction of Z-scheme heterostructure, which effectively improved the efficiency of electron–hole separation. This work proposed\u0000 a possible Z-scheme photocatalytic mechanism to elucidate the enhanced photochemical properties.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46898770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junjun Shu, ShiXin Lin, Yuehua Wu, Jun Zhu, X. Zou, Hong Zhu, Jun Gao
This study aimed to develop an animal model of pathologic regeneration of intrauterine adhesions (IUA) and assess the impact of ITGB3 and PPIF plasmid construction on the offspring of rats with IUA. Thirty female SD rats were divided into an experimental group and a control group. In the experimental group, the rats’ left and right endometrium underwent mild and severe mechanical damage using a self-made curette. The control group underwent a sham operation without endometrial injury. At various time points after surgery, uteruses were collected for analysis. Immunohistochemical staining was performed to evaluate changes in major histocompatibility complex II molecule (MHC II) and 5-bromodeoxyuridine (BrdU) in endometrial cells. The study observed the inflammatory and hyperplastic changes in endometrial tissue and the repair process in rats with different endometrial gland injuries. RNA interference (RNAi) sequences targeting the rat PPIF gene were designed and cloned into a lentivirus vector transfer plasmid, ITGB3. The number of endometrial glands decreased with increased mechanical injury. The PPIF short hairpin RNA (shRNA) fragment was successfully cloned into the lentiviral vector. After mild mechanical injury, the endometrial tissue regenerated to a basic repair level, while severe mechanical injury led to incomplete repair and tissue fibrosis, resulting in IUA.
{"title":"The Mechanism of ITGB3 and PPIF Plasmid Construction on the Offspring of Rats with Intrauterine Adhesion and Bioinformatics Analysis","authors":"Junjun Shu, ShiXin Lin, Yuehua Wu, Jun Zhu, X. Zou, Hong Zhu, Jun Gao","doi":"10.1166/sam.2023.4500","DOIUrl":"https://doi.org/10.1166/sam.2023.4500","url":null,"abstract":"This study aimed to develop an animal model of pathologic regeneration of intrauterine adhesions (IUA) and assess the impact of ITGB3 and PPIF plasmid construction on the offspring of rats with IUA. Thirty female SD rats were divided into an experimental group and a control group. In\u0000 the experimental group, the rats’ left and right endometrium underwent mild and severe mechanical damage using a self-made curette. The control group underwent a sham operation without endometrial injury. At various time points after surgery, uteruses were collected for analysis. Immunohistochemical\u0000 staining was performed to evaluate changes in major histocompatibility complex II molecule (MHC II) and 5-bromodeoxyuridine (BrdU) in endometrial cells. The study observed the inflammatory and hyperplastic changes in endometrial tissue and the repair process in rats with different endometrial\u0000 gland injuries. RNA interference (RNAi) sequences targeting the rat PPIF gene were designed and cloned into a lentivirus vector transfer plasmid, ITGB3. The number of endometrial glands decreased with increased mechanical injury. The PPIF short hairpin RNA (shRNA) fragment was successfully\u0000 cloned into the lentiviral vector. After mild mechanical injury, the endometrial tissue regenerated to a basic repair level, while severe mechanical injury led to incomplete repair and tissue fibrosis, resulting in IUA.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41917517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Min-Young Lee, Jin-Hee Kim, B. Park, Hyun-Kyu Lee, Sang-June Choi
This study proposed a novel method to remove arsenic pollutants using a magnetically separable adsorbent based on a non-toxic biopolymer. We synthesized a tannin-immobilized magnetite/graphene oxide composite (BT–Fe3O4/GO) using tannin extracted from black tea leaves (BT) and a hydrothermal method. Various characterization methods, such as Field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS), confirmed the successful synthesis of BT–Fe3O4/GO. The interactions involved in the synthesis were subsequently demonstrated. The maximum adsorption capacity (12.25 mg/g) of BT–Fe3O4/GO was approximately three times higher than that of Fe3O4/GO (3.487 mg/g), which indicated that tannin and Fe3O4 have synergetic adsorption affinities for As (V) ions. The adsorption performance of BT–Fe3O4/GO under acidic conditions was better than that under basic conditions. Based on the experimental results, the possible adsorption mechanisms of BT and Fe3O4 were described. The adsorption capacity of BT–Fe3O4/GO was approximately 80% of that of fresh BT–Fe3O4/GO, even after the third adsorption cycle. Moreover, it retained sufficient magnetic properties to collect the adsorbent after As adsorption. Owing to the synergetic adsorption performance of tannin and Fe3O4, tannin immobilization is a promising removal method for As ions, and BT–Fe3O4/GO is expected to be an alternative adsorbent for As remediation.
{"title":"Synthesis of a Tannin-Immobilized Magnetite/Graphene Oxide Composite for Magnetically Separable and Reusable Arsenic Adsorption","authors":"Min-Young Lee, Jin-Hee Kim, B. Park, Hyun-Kyu Lee, Sang-June Choi","doi":"10.1166/sam.2023.4497","DOIUrl":"https://doi.org/10.1166/sam.2023.4497","url":null,"abstract":"This study proposed a novel method to remove arsenic pollutants using a magnetically separable adsorbent based on a non-toxic biopolymer. We synthesized a tannin-immobilized magnetite/graphene oxide composite (BT–Fe3O4/GO) using tannin extracted from black\u0000 tea leaves (BT) and a hydrothermal method. Various characterization methods, such as Field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS), confirmed the successful synthesis\u0000 of BT–Fe3O4/GO. The interactions involved in the synthesis were subsequently demonstrated. The maximum adsorption capacity (12.25 mg/g) of BT–Fe3O4/GO was approximately three times higher than that of Fe3O4/GO (3.487\u0000 mg/g), which indicated that tannin and Fe3O4 have synergetic adsorption affinities for As (V) ions. The adsorption performance of BT–Fe3O4/GO under acidic conditions was better than that under basic conditions. Based on the experimental results,\u0000 the possible adsorption mechanisms of BT and Fe3O4 were described. The adsorption capacity of BT–Fe3O4/GO was approximately 80% of that of fresh BT–Fe3O4/GO, even after the third adsorption cycle. Moreover, it retained\u0000 sufficient magnetic properties to collect the adsorbent after As adsorption. Owing to the synergetic adsorption performance of tannin and Fe3O4, tannin immobilization is a promising removal method for As ions, and BT–Fe3O4/GO is expected to\u0000 be an alternative adsorbent for As remediation.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48195407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study examined the effects of Kartogenin (KGN), a small molecule compound, on intervertebral disc degeneration (IDD) cells of varying degrees. Human nucleus pulposus (NP) cells were isolated from tissues with different levels of degeneration, and the influence of KGN on cell viability, senescence, extracellular matrix (ECM), and matrix metalloproteinases (MMPs) production was investigated. Mild and severe degeneration of NP cells was induced using IL-1β. KGN increased cell viability in naturally regressive NP cells, particularly in severely regressive cells. It had no effect on healthy NP cells but promoted collagen II, aggrecan, and proliferation while decreasing p16, p21, p53, collagen I, MMP3/9, and apoptosis in naturally regressive NP cells, especially at advanced degeneration stages. Furthermore, KGN effectively prevented IL-1β-induced NP degeneration, particularly at a 10 ng/mL dose. KGN delayed NP cell degeneration, with a greater impact observed at later degeneration stages. Therefore, KGN shows potential for repairing IDD in advanced stages, but higher doses should be considered for early-stage IDD.
{"title":"Kartogenin (KGN) Reverses Intervertebral Disc Degeneration in Advanced Stages: Effects on Cell Viability, Senescence, Extracellular Matrix, and Matrix Metalloproteinases","authors":"Dongfeng Chen, Ying Li, Qiang Wang, Minghua Zhang, Shiming Huang, Peng-fei Zhan","doi":"10.1166/sam.2023.4499","DOIUrl":"https://doi.org/10.1166/sam.2023.4499","url":null,"abstract":"This study examined the effects of Kartogenin (KGN), a small molecule compound, on intervertebral disc degeneration (IDD) cells of varying degrees. Human nucleus pulposus (NP) cells were isolated from tissues with different levels of degeneration, and the influence of KGN on cell viability,\u0000 senescence, extracellular matrix (ECM), and matrix metalloproteinases (MMPs) production was investigated. Mild and severe degeneration of NP cells was induced using IL-1β. KGN increased cell viability in naturally regressive NP cells, particularly in severely regressive cells.\u0000 It had no effect on healthy NP cells but promoted collagen II, aggrecan, and proliferation while decreasing p16, p21, p53, collagen I, MMP3/9, and apoptosis in naturally regressive NP cells, especially at advanced degeneration stages. Furthermore, KGN effectively prevented IL-1β-induced\u0000 NP degeneration, particularly at a 10 ng/mL dose. KGN delayed NP cell degeneration, with a greater impact observed at later degeneration stages. Therefore, KGN shows potential for repairing IDD in advanced stages, but higher doses should be considered for early-stage IDD.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41649470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hua-Jian Zhang, Xiao-Hui Chen, Fu-Qiang Wang, Ren-Shan Chen, Ling Han
With the growing concern for energy and environmental issues, phase change materials are increasingly being recognized as a promising and eco-friendly energy storage material. However, the application of solid-liquid phase change materials is hindered by leakage issues. One effective solution to this issue is the fabrication of phase change microcapsules by encapsulating phase change materials. In this study, we designed and prepared phase change microcapsules composed of dodecane core and silica shell via interfacial polymerization, utilizing tetraethyl orthosilicate as the silicon source. The microstructure, composition, phase change properties and thermal stability of the microcapsules were analyzed using various techniques such as scanning electron microscopy, infrared spectroscopy, energy spectrum analysis, differential scanning calorimetry and thermogravimetric analysis. The findings revealed that the microcapsules had good microscopic morphology and uniform particle size of about 300–400 nm when the core–shell mass ratio was 5:5. Moreover, these microcapsules exhibited excellent phase change function and thermal stability.
{"title":"Dodecane/Silica Phase Change Microcapsules: Fabrication, Structure and Stability","authors":"Hua-Jian Zhang, Xiao-Hui Chen, Fu-Qiang Wang, Ren-Shan Chen, Ling Han","doi":"10.1166/sam.2023.4480","DOIUrl":"https://doi.org/10.1166/sam.2023.4480","url":null,"abstract":"With the growing concern for energy and environmental issues, phase change materials are increasingly being recognized as a promising and eco-friendly energy storage material. However, the application of solid-liquid phase change materials is hindered by leakage issues. One effective\u0000 solution to this issue is the fabrication of phase change microcapsules by encapsulating phase change materials. In this study, we designed and prepared phase change microcapsules composed of dodecane core and silica shell via interfacial polymerization, utilizing tetraethyl orthosilicate\u0000 as the silicon source. The microstructure, composition, phase change properties and thermal stability of the microcapsules were analyzed using various techniques such as scanning electron microscopy, infrared spectroscopy, energy spectrum analysis, differential scanning calorimetry and thermogravimetric\u0000 analysis. The findings revealed that the microcapsules had good microscopic morphology and uniform particle size of about 300–400 nm when the core–shell mass ratio was 5:5. Moreover, these microcapsules exhibited excellent phase change function and thermal stability.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42884720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetic foot ulcer (DFU) is one of the main complications of patients with diabetes and seriously endangers the health of patients. In this study, a new Co(II)-based coordination polymer with the chemical formula of {[(CH3)2NH2]0.5[Co(BTA) (TAZ)0.5 · 0.5DMA]}n (1) was synthesized and successfully loaded with arginine using the mixed ligand synthesis approach. With natural polysaccharide as raw material, HA/CMCS hydrogels loaded with arginine drug and exhibiting a metal–organic framework were prepared by chemical synthesis to control the release of arginine. The microstructure of the hydrogels was studied, and their efficacy in the treatment of DFU was evaluated. The hydrogels showed a highly porous morphology with good penetration between the pores and significantly inhibited the HG-induced decrease in cell activity and up-regulation of ROS levels to inhibit oxidative stress response. In addition, the hydrogels down-regulated the expression of TXNIP and NLRP3 to suppress the activation of NLRP3 inflammasome. Thus, these hydrogels have potential to be developed as a drug for DFU treatment.
{"title":"Mechanism of Co(II)-Based Complex Hydrogels Loaded with Arginine Against Oxidative Stress and Inflammatory Factors in Diabetic Foot Ulcers","authors":"Lina Yang, Zhicheng Zhou","doi":"10.1166/sam.2023.4495","DOIUrl":"https://doi.org/10.1166/sam.2023.4495","url":null,"abstract":"Diabetic foot ulcer (DFU) is one of the main complications of patients with diabetes and seriously endangers the health of patients. In this study, a new Co(II)-based coordination polymer with the chemical formula of {[(CH3)2NH2]0.5[Co(BTA)\u0000 (TAZ)0.5 · 0.5DMA]}n (1) was synthesized and successfully loaded with arginine using the mixed ligand synthesis approach. With natural polysaccharide as raw material, HA/CMCS hydrogels loaded with arginine drug and exhibiting a metal–organic framework\u0000 were prepared by chemical synthesis to control the release of arginine. The microstructure of the hydrogels was studied, and their efficacy in the treatment of DFU was evaluated. The hydrogels showed a highly porous morphology with good penetration between the pores and significantly inhibited\u0000 the HG-induced decrease in cell activity and up-regulation of ROS levels to inhibit oxidative stress response. In addition, the hydrogels down-regulated the expression of TXNIP and NLRP3 to suppress the activation of NLRP3 inflammasome. Thus, these hydrogels have potential to be developed\u0000 as a drug for DFU treatment.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48887879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
First-principle is used to study the structure parameters, adsorption energy, Bader charge, electronic density of states, charge-density transformation, and surface work function of CO2 molecule at various adsorption sites on the Fe(111) surface based on Density Function Theory (DFT). Results show that the CO2 molecule is absorbed on the Fe(111) surface by combining Fe–C and Fe–O multiple bonds. The type of adsorption of most configurations is chemisorption. The most stable structure is BS-Y, with an adsorption energy of −0.8115 eV. The order of stability of adsorption sites is bridge site > hcp site > fcc site > top site. Carbon dioxide mostly reacts with the uppermost two layers of Fe atoms and just partially with the lowermost two levels. In addition, the chemical bonds between CO2 molecule and Fe atoms are covalent, and the response mechanism is the hybridization of C-2s, C-2p, O-2s, and O-2p orbitals with Fe-3p, Fe-3d, Fe-4s orbitals, forming new chemical bonds. The BS-Y configuration has the smallest increment of work function, indicating that the lowest escape energy is required for the electron to escape from the surface.
{"title":"Adsorption of CO2 on the Surface of Fe(111): A First-Principles Study","authors":"Dongliang Cen","doi":"10.1166/sam.2023.4464","DOIUrl":"https://doi.org/10.1166/sam.2023.4464","url":null,"abstract":"First-principle is used to study the structure parameters, adsorption energy, Bader charge, electronic density of states, charge-density transformation, and surface work function of CO2 molecule at various adsorption sites on the Fe(111) surface based on Density Function\u0000 Theory (DFT). Results show that the CO2 molecule is absorbed on the Fe(111) surface by combining Fe–C and Fe–O multiple bonds. The type of adsorption of most configurations is chemisorption. The most stable structure is BS-Y, with an adsorption energy of −0.8115\u0000 eV. The order of stability of adsorption sites is bridge site > hcp site > fcc site > top site. Carbon dioxide mostly reacts with the uppermost two layers of Fe atoms and just partially with the lowermost two levels. In addition, the chemical bonds between CO2 molecule\u0000 and Fe atoms are covalent, and the response mechanism is the hybridization of C-2s, C-2p, O-2s, and O-2p orbitals with Fe-3p, Fe-3d, Fe-4s orbitals, forming new chemical bonds. The BS-Y configuration has the smallest increment of work function,\u0000 indicating that the lowest escape energy is required for the electron to escape from the surface.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46096461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research was aimed to investigate the effect of circPTPN4 overexpression in carboxylated single-walled carbon nanotubes (SWCNT-COOH) on renal fibrosis (RF) in mice. SWCNT was oxidized to SWCNT-COOH by concentrated sulfuric acid and nitric acid (3:1, v/v). After characterization and analysis, the effect of SWCNT-COOH on erythrocyte status was detected. The RF mice (RFM) model was induced by SWCNT-COOH, normal mice as normal control (NC), and 1×108 TU/mL lenti-NC was injected into the tail vein as negative control, and 1×108 TU/mL lenti-circPTPN4 was injected for circPTPN4 overexpression. The expression of circPTPN4 was tested by qRT-PCR. Serum creatinine (Cr), blood urea nitrogen (BUN), interleukin 1β (IL-1β), IL-6, transforming growth factor β1 (TGF-β1), kidney injury molecule 1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) were tested by ELISA. HE, Masson, and immunohistochemistry were used to detect the pathological changes of kidney tissue. The protein expressions of collagen I (COL1), fibronectin (Fn), α-SMA, TGF-β1, Smad3, p-Smad3, and Smad7 were tested by Western blot (WB). It presented SWCNT-COOH was successfully prepared by chemical oxidation method, with an average particle size of 98.1 nm and an average Zeta potential of −38.91 mV. SWCNT-COOH had good hydrophilicity, and did not cause aggregation and morphology changes of red blood cells. As against NC, the expression of circPTPN4 in RFM and lenti-NC groups was decreased; the kidney coefficient was increased, and the normal structure of the kidney was abnormal, accompanied by inflammatory cell infiltration and collagen microformation; serum Cr, BUN, IL-1β, IL-6, and TGF-β1 were increased, and KIM-1 and NGAL in renal cortex were decreased; the protein expressions of COL1, Fn, α-SMA, TGF-β1, and p-Smad3 in the kidney were increased, and the protein expression of Smad7 was decreased (P <0.05). As against RFM, the expression of circPTPN4 in lenti-circPTPN4 group was increased; the kidney coefficient decreased and the kidney structure returned to normal; COL1, Fn, α-SMA, TGF-β1, and p-Smad3 in the kidney were decreased, and that of Smad7 was increased (P <0.05). SWCNT-COOH has good blood compatibility characteristics and can induce RF in mice. The expression of circPTPN4 was down-regulated in the RFM. Overexpression of circPTPN4 can restore the normal renal function of RFM, inhibiting the inflammatory response and RF process.
{"title":"Effect and Mechanism of CircPTPN4 Expression in Carboxylated Single-Walled Carbon Nanotubes on Renal Fibrosis","authors":"Yuhan Liu, Yan Teng, Fangkun Jing, Yanning Zhang","doi":"10.1166/sam.2023.4504","DOIUrl":"https://doi.org/10.1166/sam.2023.4504","url":null,"abstract":"This research was aimed to investigate the effect of circPTPN4 overexpression in carboxylated single-walled carbon nanotubes (SWCNT-COOH) on renal fibrosis (RF) in mice. SWCNT was oxidized to SWCNT-COOH by concentrated sulfuric acid and nitric acid (3:1, v/v). After characterization\u0000 and analysis, the effect of SWCNT-COOH on erythrocyte status was detected. The RF mice (RFM) model was induced by SWCNT-COOH, normal mice as normal control (NC), and 1×108 TU/mL lenti-NC was injected into the tail vein as negative control, and 1×108 TU/mL\u0000 lenti-circPTPN4 was injected for circPTPN4 overexpression. The expression of circPTPN4 was tested by qRT-PCR. Serum creatinine (Cr), blood urea nitrogen (BUN), interleukin 1β (IL-1β), IL-6, transforming growth factor β1 (TGF-β1), kidney injury\u0000 molecule 1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) were tested by ELISA. HE, Masson, and immunohistochemistry were used to detect the pathological changes of kidney tissue. The protein expressions of collagen I (COL1), fibronectin (Fn), α-SMA, TGF-β1,\u0000 Smad3, p-Smad3, and Smad7 were tested by Western blot (WB). It presented SWCNT-COOH was successfully prepared by chemical oxidation method, with an average particle size of 98.1 nm and an average Zeta potential of −38.91 mV. SWCNT-COOH had good hydrophilicity, and did not cause aggregation\u0000 and morphology changes of red blood cells. As against NC, the expression of circPTPN4 in RFM and lenti-NC groups was decreased; the kidney coefficient was increased, and the normal structure of the kidney was abnormal, accompanied by inflammatory cell infiltration and collagen microformation;\u0000 serum Cr, BUN, IL-1β, IL-6, and TGF-β1 were increased, and KIM-1 and NGAL in renal cortex were decreased; the protein expressions of COL1, Fn, α-SMA, TGF-β1, and p-Smad3 in the kidney were increased, and the protein expression of Smad7 was decreased\u0000 (P <0.05). As against RFM, the expression of circPTPN4 in lenti-circPTPN4 group was increased; the kidney coefficient decreased and the kidney structure returned to normal; COL1, Fn, α-SMA, TGF-β1, and p-Smad3 in the kidney were decreased, and that of Smad7\u0000 was increased (P <0.05). SWCNT-COOH has good blood compatibility characteristics and can induce RF in mice. The expression of circPTPN4 was down-regulated in the RFM. Overexpression of circPTPN4 can restore the normal renal function of RFM, inhibiting the inflammatory response and\u0000 RF process.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45176448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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