Yong Wu, Si-ruo Li, Y. Liu, Liwen Yang, Weiping Lin
This study aimed to investigate the protective effect of Oleoylethanolamine (OEA) on kidney injury in diabetic nephropathy (DN) mice. Sixty C57BL/6 mice were used, and 45 mice were induced with diabetes. Thirty diabetic mice were divided into OEA low-dose and high-dose treatment groups, receiving 5 or 20 mg/kg OEA by gavage daily for 4 weeks. OEA treatment significantly improved general conditions, increased body weight, and reduced 24 h urine protein, urinary albumin, serum creatinine, and blood urea nitrogen levels. Inflammatory factors and renal inflammation were dramatically reduced after OEA intervention. OEA also increased antioxidant enzyme activity and reduced the increase of reactive oxygen species and malondialdehyde content in kidney tissues. Western blot detection revealed that OEA intervention upregulated the expression of p-AMPK and n-Nrf2 proteins in the AMPK/Nrf2 signaling pathway in renal tissue, effectively preventing the progression of DN. The study suggests that OEA’s protective effect on kidney injury in DN mice is related to the regulation of the AMPK/Nrf2 signaling pathway.
{"title":"Protective Effects of Oleoylethanolamine on Diabetic Nephropathy: Role of AMP-Activated Protein Kinase/Nuclear Factor-E2-Related Factor 2 Pathway","authors":"Yong Wu, Si-ruo Li, Y. Liu, Liwen Yang, Weiping Lin","doi":"10.1166/sam.2023.4460","DOIUrl":"https://doi.org/10.1166/sam.2023.4460","url":null,"abstract":"This study aimed to investigate the protective effect of Oleoylethanolamine (OEA) on kidney injury in diabetic nephropathy (DN) mice. Sixty C57BL/6 mice were used, and 45 mice were induced with diabetes. Thirty diabetic mice were divided into OEA low-dose and high-dose treatment groups,\u0000 receiving 5 or 20 mg/kg OEA by gavage daily for 4 weeks. OEA treatment significantly improved general conditions, increased body weight, and reduced 24 h urine protein, urinary albumin, serum creatinine, and blood urea nitrogen levels. Inflammatory factors and renal inflammation were dramatically\u0000 reduced after OEA intervention. OEA also increased antioxidant enzyme activity and reduced the increase of reactive oxygen species and malondialdehyde content in kidney tissues. Western blot detection revealed that OEA intervention upregulated the expression of p-AMPK and n-Nrf2 proteins in\u0000 the AMPK/Nrf2 signaling pathway in renal tissue, effectively preventing the progression of DN. The study suggests that OEA’s protective effect on kidney injury in DN mice is related to the regulation of the AMPK/Nrf2 signaling pathway.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43431779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CircRNAs are a class of endogenous non-coding RNAs. Therefore, circRNA has the potential to be used as a molecular marker in tumor therapy. The aim of this study is to detect the important functions of circ_0001806 on the progress and immune escape NSCLC. RT-QPCR was used to calculate the levels of circRNA and miRNA. Immunoblotting was employed to measure the protein level of PDL1. MTT and transwell assays were applied to detect the cell proliferation, migration and invasion. We found that knockdown circ_0001806 inhibited cell proliferation, migration, invasion and suppressed PDL1 expression of NSCLC. Circ_0001806 directly binding to miR-1236, and miR-1236 could partially reversed the functions of silencing circ_0001806. Circ_0001806 regulated cell proliferation, migration, invasion, and tumor immune escape by sponging miR-1236, providing a new perspective for exploring targeted therapies for NSCLC.
{"title":"Exploring the Role of Circular RNA (Circ_0001806) in Non-Small Cell Lung Cancer Progression and Immune Evasion through miRNA Regulation","authors":"Yu-hong Guo, Lingchen Meng, Xiao Liang, Zhaohui Hou, Wenping Leng, Yue Sun, Yuanshuo Yang, Zhenbo Hou, P. Zhang","doi":"10.1166/sam.2023.4470","DOIUrl":"https://doi.org/10.1166/sam.2023.4470","url":null,"abstract":"CircRNAs are a class of endogenous non-coding RNAs. Therefore, circRNA has the potential to be used as a molecular marker in tumor therapy. The aim of this study is to detect the important functions of circ_0001806 on the progress and immune escape NSCLC. RT-QPCR was used to calculate\u0000 the levels of circRNA and miRNA. Immunoblotting was employed to measure the protein level of PDL1. MTT and transwell assays were applied to detect the cell proliferation, migration and invasion. We found that knockdown circ_0001806 inhibited cell proliferation, migration, invasion and suppressed\u0000 PDL1 expression of NSCLC. Circ_0001806 directly binding to miR-1236, and miR-1236 could partially reversed the functions of silencing circ_0001806. Circ_0001806 regulated cell proliferation, migration, invasion, and tumor immune escape by sponging miR-1236, providing a new perspective for\u0000 exploring targeted therapies for NSCLC.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47570510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research discussed the value of regenerated wild Antheraea pernyi silk fibroin (RWSF)/polyvinyl alcohol (PVA) nanofiber scaffold (NFS) in repairing the calcaneal tendon defect (CTD). RWSF was prepared by saturated salt solution (SSS) method, and then RWSF/PVA NFS was prepared by electrospinning using RWSF and PVA as raw materials. Fourier transform infrared spectroscopy (FTIS) was applied to detect the characteristic absorption spectra of WSF, RWSF, and RWSF/PVA. The ultimate tensile strength (UTS) and elongation at break (BE) of RWSF/PVA NFS were analyzed by mechanical tester. The cytotoxicity of RWSF/PVA NFS was determined by MTT assay. 18 SD rats were randomly rolled into an operation group, control group, and experimental group, with 6 rats in each. Meanwhile, 27 rats were randomly grouped into three: blank group, model group, and experimental group. HE staining, Masson staining, and biomechanical properties of the regenerated fibers were analyzed in the calcaneal tendon tissues (CTTs) of rats in different groups. Expressions of tendon-related genes and inflammatory factors in CTTs in various groups were compared by RT-PCR. The results revealed that the UTS and BE of PVA and RWSF/PVA were much higher than those of natural acellular tendon (P <0.01). On day 15 after operation, the hair in the incision area of rats in the Ope, Con, and Exp groups grew normally. The implanted RWSF/PVA NFS in the Exp group adhered closely to the surrounding muscle tissue and degraded gradually, and there were still trace inflammatory cells at the junction. The tendon cross sectional area (CSA) in the Model group and RWSF/PVA group was greatly higher based on that in the Blank group (P <0.05), and the UTS in of Model group was much higher than that in the Blank group but lower to the Model group, showing great differences with P <0.05. The Collagen I, Collagen III, TGF-β1, BGN, and TNMD in CTTs in the RWSF/PVA group were higher to the Model group 2 months ago (P <0.05); while Collagen I, TGF-β1, BGN, and TNMD were still much higher 3 months later (P <0.01) but Collagen III was lower with an obvious difference (P <0.05). At 5 months, IL-1β and TNF-α in the RWSF/PVA group were greatly lower in contrast to the model group, presenting extremely obvious differences (P <0.001). The results indicated that the RWSF/PVA NFS exhibited a good biocompatibility, can accelerate the collagen secretion, promote TGF-β1, inhibit IL-1β and TNF-α factors, thus being conductive to repair of CTD. In conclusion, RWSF/PVA NFS possessed a good biocompatibility, can promote collagen secretion, elevate the TGF-β1, and inhibit IL-1β and TNF-α factors to participate in calcaneal CTD repair, showing a high value in repair of CTD.
{"title":"Feasibility and Safety of Regenerated Wild Antheraea pernyi Silk Fibroin/Polyvinyl Alcohol Scaffold in Repair of Calcaneal Tendon Defects","authors":"Lihe Cao, Wen-guang Tian, Baocheng Li, Fuchao You, Jiahe Hang, Duan Hao, Donghuang He","doi":"10.1166/sam.2023.4462","DOIUrl":"https://doi.org/10.1166/sam.2023.4462","url":null,"abstract":"This research discussed the value of regenerated wild Antheraea pernyi silk fibroin (RWSF)/polyvinyl alcohol (PVA) nanofiber scaffold (NFS) in repairing the calcaneal tendon defect (CTD). RWSF was prepared by saturated salt solution (SSS) method, and then RWSF/PVA NFS was prepared\u0000 by electrospinning using RWSF and PVA as raw materials. Fourier transform infrared spectroscopy (FTIS) was applied to detect the characteristic absorption spectra of WSF, RWSF, and RWSF/PVA. The ultimate tensile strength (UTS) and elongation at break (BE) of RWSF/PVA NFS were analyzed by mechanical\u0000 tester. The cytotoxicity of RWSF/PVA NFS was determined by MTT assay. 18 SD rats were randomly rolled into an operation group, control group, and experimental group, with 6 rats in each. Meanwhile, 27 rats were randomly grouped into three: blank group, model group, and experimental group.\u0000 HE staining, Masson staining, and biomechanical properties of the regenerated fibers were analyzed in the calcaneal tendon tissues (CTTs) of rats in different groups. Expressions of tendon-related genes and inflammatory factors in CTTs in various groups were compared by RT-PCR. The results\u0000 revealed that the UTS and BE of PVA and RWSF/PVA were much higher than those of natural acellular tendon (P <0.01). On day 15 after operation, the hair in the incision area of rats in the Ope, Con, and Exp groups grew normally. The implanted RWSF/PVA NFS in the Exp group adhered\u0000 closely to the surrounding muscle tissue and degraded gradually, and there were still trace inflammatory cells at the junction. The tendon cross sectional area (CSA) in the Model group and RWSF/PVA group was greatly higher based on that in the Blank group (P <0.05), and the UTS in\u0000 of Model group was much higher than that in the Blank group but lower to the Model group, showing great differences with P <0.05. The Collagen I, Collagen III, TGF-β1, BGN, and TNMD in CTTs in the RWSF/PVA group were higher to the Model group 2 months ago (P <0.05);\u0000 while Collagen I, TGF-β1, BGN, and TNMD were still much higher 3 months later (P <0.01) but Collagen III was lower with an obvious difference (P <0.05). At 5 months, IL-1β and TNF-α in the RWSF/PVA group were greatly lower in contrast\u0000 to the model group, presenting extremely obvious differences (P <0.001). The results indicated that the RWSF/PVA NFS exhibited a good biocompatibility, can accelerate the collagen secretion, promote TGF-β1, inhibit IL-1β and TNF-α factors, thus\u0000 being conductive to repair of CTD. In conclusion, RWSF/PVA NFS possessed a good biocompatibility, can promote collagen secretion, elevate the TGF-β1, and inhibit IL-1β and TNF-α factors to participate in calcaneal CTD repair, showing a high value in repair\u0000 of CTD.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41864127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Triptolide (TP) has potential adoption value in the treatment of nephropathy, but its poor water solubility causes toxicity and side effects to various degrees in the kidney, liver, and other organs. In this research, TP-mesoscale nanoparticles (TP-MNPs) were synthesized by the amide reaction of poly(lactic-co-glycolic acid) (PLGA) and methoxy polyethylene glycol amine (mPEG-NH2) as drug carrier materials. The structure of TP-MNPs was characterized by 1H NMR, scanning electron microscopy (SEM), and zeta potential using a nanoparticle potential analyzer. Liquid chromatography–mass spectrometry (HPLC–MS) was utilized to determine the content of TP in TP-MNPs and calculate the entrapment rate (ER) and drug loading (DL) of TP-MNPs. The cytotoxicity of TP-MNPs was detected by CCK8. Eight mice were enrolled in Blank group (no treatment), and the other 24 model group animals were rolled into model group (DM), TP group (TP intervention), and TP-MNP group (TP-MNPs intervention) randomly, with eight mice in each group. The urine protein content, serum albumin (Alb), blood glucose (Glu), creatinine (Cre), total cholesterol (TCHO), and triglyceride (TG) contents of mice in various groups after intervention were compared. The results revealed that average diameter of MNPs was (379.6±26.44) nm, that of TP-MNPs was (424.3±56.29) nm, and average zeta potentials of TP, MNPs, and TP-MNPs were (−28.29±3.85) mV, (−15.51±1.79) mV, and (−13.45±1.81) mV, respectively. The curve changed with TP concentration. With increasing TP concentration, the ER of TP-MNPs decreased drastically, and the DL demonstrated a drastic upward trend. Furthermore, the cell activities of HK-2, NRK-52E, LO2, and AML-12 treated with TP-MNPs were higher versus TP group (P <0.05). Urine protein content, body weight, renal index, serum Glu, TCHO, and TG content in the DM group were markedly superior to Blank group (P <0.001), while urine protein content in TP-MNP group was considerably inferior to the DM group (P <0.01). Alb and Cre in the serum of mice in DM group were substantially lower versus Blank group (P <0.01), while those in TP group were lower than Blank group (P <0.05). Alb and Cre in TP-MNP group were superior to DM group (P <0.05). In summary, TP-MNPs prepared in this research had ideal biocompatibility, could effectively improve urinary protein, body weight, renal index, and serum biochemical indexes caused by DM, and had a certain therapeutic effect on diabetic nephropathy (DN) mice.
{"title":"Therapeutic Effect of Triptolide Polymer Mesoscale Nanoparticles on Diabetic Nephropathy Mice","authors":"Min Guo, P. Cheng","doi":"10.1166/sam.2023.4461","DOIUrl":"https://doi.org/10.1166/sam.2023.4461","url":null,"abstract":"Triptolide (TP) has potential adoption value in the treatment of nephropathy, but its poor water solubility causes toxicity and side effects to various degrees in the kidney, liver, and other organs. In this research, TP-mesoscale nanoparticles (TP-MNPs) were synthesized by the amide\u0000 reaction of poly(lactic-co-glycolic acid) (PLGA) and methoxy polyethylene glycol amine (mPEG-NH2) as drug carrier materials. The structure of TP-MNPs was characterized by 1H NMR, scanning electron microscopy (SEM), and zeta potential using a nanoparticle potential analyzer.\u0000 Liquid chromatography–mass spectrometry (HPLC–MS) was utilized to determine the content of TP in TP-MNPs and calculate the entrapment rate (ER) and drug loading (DL) of TP-MNPs. The cytotoxicity of TP-MNPs was detected by CCK8. Eight mice were enrolled in Blank group (no treatment),\u0000 and the other 24 model group animals were rolled into model group (DM), TP group (TP intervention), and TP-MNP group (TP-MNPs intervention) randomly, with eight mice in each group. The urine protein content, serum albumin (Alb), blood glucose (Glu), creatinine (Cre), total cholesterol (TCHO),\u0000 and triglyceride (TG) contents of mice in various groups after intervention were compared. The results revealed that average diameter of MNPs was (379.6±26.44) nm, that of TP-MNPs was (424.3±56.29) nm, and average zeta potentials of TP, MNPs, and TP-MNPs were (−28.29±3.85)\u0000 mV, (−15.51±1.79) mV, and (−13.45±1.81) mV, respectively. The curve changed with TP concentration. With increasing TP concentration, the ER of TP-MNPs decreased drastically, and the DL demonstrated a drastic upward trend. Furthermore, the cell activities of HK-2,\u0000 NRK-52E, LO2, and AML-12 treated with TP-MNPs were higher versus TP group (P <0.05). Urine protein content, body weight, renal index, serum Glu, TCHO, and TG content in the DM group were markedly superior to Blank group (P <0.001), while urine protein content in TP-MNP\u0000 group was considerably inferior to the DM group (P <0.01). Alb and Cre in the serum of mice in DM group were substantially lower versus Blank group (P <0.01), while those in TP group were lower than Blank group (P <0.05). Alb and Cre in TP-MNP group were superior\u0000 to DM group (P <0.05). In summary, TP-MNPs prepared in this research had ideal biocompatibility, could effectively improve urinary protein, body weight, renal index, and serum biochemical indexes caused by DM, and had a certain therapeutic effect on diabetic nephropathy (DN) mice.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43666540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bone tissue is one of the most transplanted tissues. During transplantation, additive manufacturing enables fast and efficient production and fabrication of shaped external implants, and designing porous interconnected bionic external implants is the focus and difficulty of bone tissue engineering. Based on Voronoi geometry to design a bone tissue structure with hole position gradient—Voronoi gradient structure (VFGM, Voronoi functional gradient materials), the design hole center conforms to the uniform gradient probability spatial distribution, and the boundary in 3D Voronoi is calculated with the hole center as the bone trabecular skeletal. It can be used to design a variety of heterogeneous structures to match with the damaged location. The heteromorphic VFGM structure is highly similar to the human bone structure and can provide geometric design for bone tissue engineering external implants.
{"title":"Design and Geometric Characterization of Three-Dimensional Gradient Heterogeneous Bone Tissue Structures Based on Voronoi","authors":"Hong-Liang Pei, Qing-Wen Fan, Dong Ma, Yifei Yang","doi":"10.1166/sam.2023.4400","DOIUrl":"https://doi.org/10.1166/sam.2023.4400","url":null,"abstract":"Bone tissue is one of the most transplanted tissues. During transplantation, additive manufacturing enables fast and efficient production and fabrication of shaped external implants, and designing porous interconnected bionic external implants is the focus and difficulty of bone tissue\u0000 engineering. Based on Voronoi geometry to design a bone tissue structure with hole position gradient—Voronoi gradient structure (VFGM, Voronoi functional gradient materials), the design hole center conforms to the uniform gradient probability spatial distribution, and the boundary in\u0000 3D Voronoi is calculated with the hole center as the bone trabecular skeletal. It can be used to design a variety of heterogeneous structures to match with the damaged location. The heteromorphic VFGM structure is highly similar to the human bone structure and can provide geometric design\u0000 for bone tissue engineering external implants.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44947229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Lung cancer is the most important disease that endangers human health worldwide. High research value exists for liquid biopsy in predicting treatment response, early diagnosis of disease recurrence, and real-time dynamic detection of tumor evolution, and each one have their own unique advantages and drawbacks. Methods: We used a novel combined Epcam immunomagnetic liposome bead (Ep-IML) and Vimentin immunomagnetic liposome bead (Vi-IML) tumor cell enrichment strategy to capture CTCs from 30 lung adenocarcinoma (LAC) patients and then performed high-throughput sequencing with Circulating tumor cell DNA (ctcDNA) and ctDNA to understand the genetic variants of the patients. Results: The modified CTC enrichment efficiency was significantly improved and the mean value of CTCs enriched when Ep-IML combined with Vi-IML is 11.78/7.5 mL. The results of genomic analysis of CTC in lung adenocarcinoma showed that the five most frequently mutated genes were EGFR, TP53, KRAS, ALK, BRAF. And results of ctDNA gene analysis in lung adenocarcinoma patients showed that the five most frequently mutated genes were EGFR, AKT1, TP53, DDR2, and FGFR3. NGS analysis demonstrated that variations in the genetic profile revealed by the liquid biopsy might be increased by combining tests with CTC and ctDNA. Conclusion: We have developed a customized CTC enrichment identification system. CTCs could be an ideal complement to ctDNA and have important clinical applications in guiding clinical dosing and individualized therapy, combined CTC and ctDNA assays could detect as many drug-available targets as possible for a patient in a single trial.
{"title":"Combination Analyses Next Generation Sequencing of Lung Adenocarcinoma ctDNA and CTCs Based on Multi-Site Immunomagnetic Beads","authors":"Jia Li, Jun Ding, F. Xu","doi":"10.1166/sam.2023.4405","DOIUrl":"https://doi.org/10.1166/sam.2023.4405","url":null,"abstract":"Background: Lung cancer is the most important disease that endangers human health worldwide. High research value exists for liquid biopsy in predicting treatment response, early diagnosis of disease recurrence, and real-time dynamic detection of tumor evolution, and each one\u0000 have their own unique advantages and drawbacks. Methods: We used a novel combined Epcam immunomagnetic liposome bead (Ep-IML) and Vimentin immunomagnetic liposome bead (Vi-IML) tumor cell enrichment strategy to capture CTCs from 30 lung adenocarcinoma (LAC) patients and then performed\u0000 high-throughput sequencing with Circulating tumor cell DNA (ctcDNA) and ctDNA to understand the genetic variants of the patients. Results: The modified CTC enrichment efficiency was significantly improved and the mean value of CTCs enriched when Ep-IML combined with Vi-IML is 11.78/7.5\u0000 mL. The results of genomic analysis of CTC in lung adenocarcinoma showed that the five most frequently mutated genes were EGFR, TP53, KRAS, ALK, BRAF. And results of ctDNA gene analysis in lung adenocarcinoma patients showed that the five most frequently mutated genes were EGFR, AKT1, TP53,\u0000 DDR2, and FGFR3. NGS analysis demonstrated that variations in the genetic profile revealed by the liquid biopsy might be increased by combining tests with CTC and ctDNA. Conclusion: We have developed a customized CTC enrichment identification system. CTCs could be an ideal complement\u0000 to ctDNA and have important clinical applications in guiding clinical dosing and individualized therapy, combined CTC and ctDNA assays could detect as many drug-available targets as possible for a patient in a single trial.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41670032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaodong Song, Shanshan Guo, Mei Wang, Rui Fan, Yang Li, Qiquan Yu, Qiuli Bao, Chunxiao Wu, Ze-Quan Zhang, Kaiyao Zhang
This study aimed to investigate the inhibitory effects of Iphigenia indica extracts on lung adenocarcinoma A549 cells through the PI3K/AKT1 signaling pathway. The researchers treated A549 cells with different concentrations of Iphigenia indica extracts and conducted various assays. The results showed that the viability of A549 cells decreased with increasing concentration of Iphigenia indica extracts. Iphigenia indica extracts and PI3K/AKT inhibitor had a higher inhibitory rate of cell proliferation and colony formation, reduced migration and invasion, and induced apoptosis in A549 cells compared to the control group. Furthermore, Iphigenia indica extracts and PI3K/AKT inhibitor reduced the protein levels of Bcl-2, PI3K, and AKT1 and increased the level of Bax. The findings suggest that Iphigenia indica extracts may inhibit malignant biological behaviors of lung adenocarcinoma cells through the PI3K/AKT1 signaling pathway by inducing apoptosis, inhibiting proliferation, migration and invasion, and regulating the expression of Bcl-2, Bax, PI3K, and AKT1. Overall, Iphigenia indica extracts may have potential as a therapeutic agent for lung adenocarcinoma.
{"title":"Targeting the Phosphatidylinositol 3-Kinase/AKT1 Signaling Pathway: Iphigenia indica Extracts Induce Apoptosis in Lung Adenocarcinoma Cells","authors":"Xiaodong Song, Shanshan Guo, Mei Wang, Rui Fan, Yang Li, Qiquan Yu, Qiuli Bao, Chunxiao Wu, Ze-Quan Zhang, Kaiyao Zhang","doi":"10.1166/sam.2023.4459","DOIUrl":"https://doi.org/10.1166/sam.2023.4459","url":null,"abstract":"This study aimed to investigate the inhibitory effects of Iphigenia indica extracts on lung adenocarcinoma A549 cells through the PI3K/AKT1 signaling pathway. The researchers treated A549 cells with different concentrations of Iphigenia indica extracts and conducted various assays. The results showed that the viability of A549 cells decreased with increasing concentration of Iphigenia indica extracts. Iphigenia indica extracts and PI3K/AKT inhibitor had a higher inhibitory rate of cell proliferation and colony formation, reduced migration and invasion, and induced apoptosis in A549 cells compared to the control group. Furthermore, Iphigenia indica extracts and PI3K/AKT inhibitor reduced the protein levels of Bcl-2, PI3K, and AKT1 and increased the level of Bax. The findings suggest that Iphigenia indica extracts may inhibit malignant biological behaviors of lung adenocarcinoma cells through the PI3K/AKT1 signaling pathway by inducing apoptosis, inhibiting proliferation, migration and invasion, and regulating the expression of Bcl-2, Bax, PI3K, and AKT1. Overall, Iphigenia indica extracts may have potential as a therapeutic agent for lung adenocarcinoma.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49092182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Danyang Li, Dan Li, Zengchao Tang, Wenyao Li, Enping Lai, Wei Zhao, H. Fouad, Zufesha NoorulHuda Khan, Sheheryar Munir, Kaile Zhang
Urinary calculus is a very common disease in urology. Ureteral stent is popularly used in patients after surgeries to protect the ureter. Indwelling ureteral stent inevitably causes stent syndrome for the reason of foreign body stimulation and rough stent surface. Clinicaly, oral administration of solifenacine is a solution to relieve the symptoms, however it is with several complications. In our study, ureteral stent was coated with bioactive solifenacine in polyethylene glycol diacrylate (PEGMA) and polyvinylpyrrolidone (PVP) to realize super smooth surface and controlled release of solifenacine. Scanning electron microscopy (SEM) and friction test of solifenacin-loaded super-smooth stent (SSSS) revealed its smooth surface. Fourier transform infrared spectroscopy and controlled release test showed its solifenacin delivering and controlled releasing. The polymerase chain reaction (PCR) showed inhibited α-smooth mucle actin expression in SSSS treated smooth muscle cells. The SSSS was applied in male New Zealand rabbits and revealed the effect of inhibiting the abnormal contraction of bladders. The Histology of SSSS treated bladder revealed a diastolic muscle layer of bladder. The SSSS after implantation showed smoother surface and less calcium deposition. In conclusion, it’s demonstrated that the SSSS has the efficacy of relieving stent syndrome and potential for clinical translation and application.
{"title":"Polyethylene Glycol Diacrylate (PEGMA) and Polyvinylpyrrolidone (PVP) Delivering Solifenacine as Super Smooth Coating of Ureteral Stent to Relieve Stent Syndrome: In Vitro and In Vivo Study","authors":"Danyang Li, Dan Li, Zengchao Tang, Wenyao Li, Enping Lai, Wei Zhao, H. Fouad, Zufesha NoorulHuda Khan, Sheheryar Munir, Kaile Zhang","doi":"10.1166/sam.2023.4453","DOIUrl":"https://doi.org/10.1166/sam.2023.4453","url":null,"abstract":"Urinary calculus is a very common disease in urology. Ureteral stent is popularly used in patients after surgeries to protect the ureter. Indwelling ureteral stent inevitably causes stent syndrome for the reason of foreign body stimulation and rough stent surface. Clinicaly, oral administration\u0000 of solifenacine is a solution to relieve the symptoms, however it is with several complications. In our study, ureteral stent was coated with bioactive solifenacine in polyethylene glycol diacrylate (PEGMA) and polyvinylpyrrolidone (PVP) to realize super smooth surface and controlled release\u0000 of solifenacine. Scanning electron microscopy (SEM) and friction test of solifenacin-loaded super-smooth stent (SSSS) revealed its smooth surface. Fourier transform infrared spectroscopy and controlled release test showed its solifenacin delivering and controlled releasing. The polymerase\u0000 chain reaction (PCR) showed inhibited α-smooth mucle actin expression in SSSS treated smooth muscle cells. The SSSS was applied in male New Zealand rabbits and revealed the effect of inhibiting the abnormal contraction of bladders. The Histology of SSSS treated bladder revealed\u0000 a diastolic muscle layer of bladder. The SSSS after implantation showed smoother surface and less calcium deposition. In conclusion, it’s demonstrated that the SSSS has the efficacy of relieving stent syndrome and potential for clinical translation and application.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46388821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic and acute wounds pose a huge burden on patients and health care systems. Early diagnosis and prompt treatment is essential in preventing further complications such as limb amputation and infection. Recent progress in our understanding of different wounds’ pathophysiology, has resulted in developing different drug delivery vehicles to target different phases of wound healing. During the past decade, microspheres and nanospheres have gained significant attention in drug delivering wound dressings. These vehicles have gained popularity largely due their biocompatibility, biodegradability, their high capacity to deliver various drug types, and long term sustained release profile. In the current review, we will discuss the challenges and prospects of microsphere and nanosphere-based drug delivery systems in wound healing.
{"title":"Nanosphere and Microsphere-Based Drug Delivery Systems for Wound Healing Applications: A Review","authors":"Ningjuan Han, Huanle Fang, Rui Niu","doi":"10.1166/sam.2023.4446","DOIUrl":"https://doi.org/10.1166/sam.2023.4446","url":null,"abstract":"Chronic and acute wounds pose a huge burden on patients and health care systems. Early diagnosis and prompt treatment is essential in preventing further complications such as limb amputation and infection. Recent progress in our understanding of different wounds’ pathophysiology,\u0000 has resulted in developing different drug delivery vehicles to target different phases of wound healing. During the past decade, microspheres and nanospheres have gained significant attention in drug delivering wound dressings. These vehicles have gained popularity largely due their biocompatibility,\u0000 biodegradability, their high capacity to deliver various drug types, and long term sustained release profile. In the current review, we will discuss the challenges and prospects of microsphere and nanosphere-based drug delivery systems in wound healing.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47868648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Dong, Xiaowei Liu, Suo Wang, Xiujun Chen, Kun Zhang, Ying Zhang
To investigate the effects of micro ribonucleic acid (miR)-125a-5p on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells and its possible mechanism. With NSCLC A549 cells as the experimental research objects, transfection reagent was employed to transfect miR-125a-5p NC group, miR-125a-5p mimic group and miR-125a-5p siRNA group into A549 cells. qRT-PCR and cloning assays were conducted to detect the level of miR125a-5p in A549 cells and the effect of miR125a-5p on the proliferation of A549 cells. The effect of miR-125a-5p on apoptosis of A549 cells was detected via FCM. Additionally, the effects of miR-125a-5p on the mRNA and protein expressions of PI3K and AKT and the expressions of MMP-2 and MMP-9 in A549 cells were determined through qRT-PCR, Western blotting and immunohistochemistry, respectively. Compared with those in miR-125a-5p NC group, the proliferation ability of A549 cells was improved, their apoptosis rate was significantly decreased, and the mRNA and protein levels of PI3K and AKT and the levels of MMP-2 and MMP-9 in A549 cells were increased in miR-125a-5p siRNA group, whereas they showed totally opposite tendencies in miR-125a-5p mimic group. miR-125a-5p overexpression can hinder A549 cell growth, boost apoptosis, and reduce MMP-2 and MMP-9 levels via the PI3K/AKT/MMP pathway.
{"title":"microRNA-125a-5p as a Tumor Suppressor in Non-Small Cell Lung Cancer (NSCLC): Inhibition of Proliferation and Induction of Apoptosis via P13K/AKT/MMP Signaling Pathway","authors":"B. Dong, Xiaowei Liu, Suo Wang, Xiujun Chen, Kun Zhang, Ying Zhang","doi":"10.1166/sam.2023.4457","DOIUrl":"https://doi.org/10.1166/sam.2023.4457","url":null,"abstract":"To investigate the effects of micro ribonucleic acid (miR)-125a-5p on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells and its possible mechanism. With NSCLC A549 cells as the experimental research objects, transfection reagent was employed to transfect miR-125a-5p\u0000 NC group, miR-125a-5p mimic group and miR-125a-5p siRNA group into A549 cells. qRT-PCR and cloning assays were conducted to detect the level of miR125a-5p in A549 cells and the effect of miR125a-5p on the proliferation of A549 cells. The effect of miR-125a-5p on apoptosis of A549 cells was\u0000 detected via FCM. Additionally, the effects of miR-125a-5p on the mRNA and protein expressions of PI3K and AKT and the expressions of MMP-2 and MMP-9 in A549 cells were determined through qRT-PCR, Western blotting and immunohistochemistry, respectively. Compared with those in miR-125a-5p NC\u0000 group, the proliferation ability of A549 cells was improved, their apoptosis rate was significantly decreased, and the mRNA and protein levels of PI3K and AKT and the levels of MMP-2 and MMP-9 in A549 cells were increased in miR-125a-5p siRNA group, whereas they showed totally opposite tendencies\u0000 in miR-125a-5p mimic group. miR-125a-5p overexpression can hinder A549 cell growth, boost apoptosis, and reduce MMP-2 and MMP-9 levels via the PI3K/AKT/MMP pathway.","PeriodicalId":21671,"journal":{"name":"Science of Advanced Materials","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46083215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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