Sensors International最新文献

英文中文

Palm-size wireless piezoelectric immune-biosensing system for rapid E. coli O157:H7 detection 手掌大小的无线压电免疫生物传感系统，用于快速检测大肠杆菌O157:H7

Sensors International

Pub Date : 2025-01-01 DOI: 10.1016/j.sintl.2025.100341

Yang Tian , Lisa Kelso , Yiting Xiao , Chaitanya Pallerla , Ramesh Bist , Siavash Mahmoudi , Ziyu Liu , Haizheng Xiong , Jeyam Subbiah , Terry Howell , Dongyi Wang

Rapid and accurate detection of Escherichia coli O157:H7 is essential for ensuring public health, food safety, and environmental monitoring. Traditional detection methods are often time-consuming and require specialized laboratory infrastructure, limiting their practicality for on-site applications. In this study, we present a palm-sized, wireless piezoelectric immunosensor system designed for the swift and sensitive detection of E. coli O157:H7. The system integrates a gold-coated quartz crystal microbalance (QCM) sensor functionalized with protein A-immobilized antibodies, a polydimethylsiloxane (PDMS) microfluidic channel for efficient sample handling, and a flexible loop antenna for wireless signal transmission. Real-time data is transmitted via Bluetooth to a smartphone application, enhancing user interaction and portability. Sensitivity tests demonstrated a proportional increase in frequency shift corresponding to E. coli O157:H7 concentrations, achieving a detection limit as low as 10² CFU ml⁻¹ in field samples. Selectivity tests confirmed minimal cross-reactivity with other bacteria, highlighting the sensor's specificity. The incorporation of immuno-nanobeads amplified the signal, enhancing the system's ability to detect low bacterial concentrations. The portable, self-powered system offers a practical solution for rapid, on-site pathogen detection, addressing critical needs in food safety and environmental monitoring. By enabling timely and accurate detection of contaminants, this technology has the potential to significantly impact public health by reducing the incidence of foodborne illnesses, safeguarding environmental resources, and enhancing overall safety and health outcomes.

快速准确地检测O157:H7大肠杆菌对于确保公共卫生、食品安全和环境监测至关重要。传统的检测方法往往耗时且需要专门的实验室基础设施，限制了其现场应用的实用性。在这项研究中，我们提出了一个手掌大小的无线压电免疫传感器系统，用于快速灵敏地检测大肠杆菌O157:H7。该系统集成了一个带有蛋白a固定化抗体的镀金石英晶体微天平（QCM）传感器，一个用于高效样品处理的聚二甲基硅氧烷（PDMS）微流控通道，以及一个用于无线信号传输的柔性环形天线。实时数据通过蓝牙传输到智能手机应用程序，增强了用户交互和便携性。敏感性试验表明，频率漂移与大肠杆菌O157:H7浓度成比例增加，在现场样品中达到低至102 CFU ml−1的检测限。选择性测试证实了与其他细菌的最小交叉反应性，突出了传感器的特异性。免疫纳米粒的结合放大了信号，增强了系统检测低细菌浓度的能力。便携式自供电系统为快速现场病原体检测提供了实用的解决方案，解决了食品安全和环境监测的关键需求。这项技术能够及时、准确地检测污染物，有可能通过减少食源性疾病的发病率、保护环境资源和提高总体安全和健康结果，对公众健康产生重大影响。

{"title":"Palm-size wireless piezoelectric immune-biosensing system for rapid E. coli O157:H7 detection","authors":"Yang Tian , Lisa Kelso , Yiting Xiao , Chaitanya Pallerla , Ramesh Bist , Siavash Mahmoudi , Ziyu Liu , Haizheng Xiong , Jeyam Subbiah , Terry Howell , Dongyi Wang","doi":"10.1016/j.sintl.2025.100341","DOIUrl":"10.1016/j.sintl.2025.100341","url":null,"abstract":"<div><div>Rapid and accurate detection of <em>Escherichia coli O157:H7</em> is essential for ensuring public health, food safety, and environmental monitoring. Traditional detection methods are often time-consuming and require specialized laboratory infrastructure, limiting their practicality for on-site applications. In this study, we present a palm-sized, wireless piezoelectric immunosensor system designed for the swift and sensitive detection of <em>E. coli O157:H7</em>. The system integrates a gold-coated quartz crystal microbalance (QCM) sensor functionalized with protein A-immobilized antibodies, a polydimethylsiloxane (PDMS) microfluidic channel for efficient sample handling, and a flexible loop antenna for wireless signal transmission. Real-time data is transmitted via Bluetooth to a smartphone application, enhancing user interaction and portability. Sensitivity tests demonstrated a proportional increase in frequency shift corresponding to <em>E. coli O157:H7</em> concentrations, achieving a detection limit as low as 10<sup>2</sup> CFU ml<sup>−1</sup> in field samples. Selectivity tests confirmed minimal cross-reactivity with other bacteria, highlighting the sensor's specificity. The incorporation of immuno-nanobeads amplified the signal, enhancing the system's ability to detect low bacterial concentrations. The portable, self-powered system offers a practical solution for rapid, on-site pathogen detection, addressing critical needs in food safety and environmental monitoring. By enabling timely and accurate detection of contaminants, this technology has the potential to significantly impact public health by reducing the incidence of foodborne illnesses, safeguarding environmental resources, and enhancing overall safety and health outcomes.</div></div>","PeriodicalId":21733,"journal":{"name":"Sensors International","volume":"6 ","pages":"Article 100341"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a sugar-derived silver nanoparticle-based electrochemical immunosensor for sensitive D-dimer detection 基于糖源银纳米粒子的d -二聚体灵敏检测电化学免疫传感器的研制

Sensors International

Pub Date : 2025-01-01 DOI: 10.1016/j.sintl.2025.100332

Fatma Ozturk Kirbay , İdris Yazgan , Dilek Odaci

D-dimer (DD) is a key marker of coagulation and fibrinolysis activation. Clinicians can make important decisions with the aid of quick analysis and quantification, ideally at the patient's bedside. Electrochemical immunosensors are effective point-of-care technologies to address these issues. Here, we describe the development of a simple electrochemical immunosensor to detect DD. The immunosensor is constructed by electrodeposition of lactose methoxide aniline silver nanoparticles (LMA-AgNPs) on a screen-printed carbon electrode (SPCE). The LMA-AgNP and SPCE/LMA-AgNP were characterized by Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of the SPCE/LMA-AgNP was examined using electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Then, Anti-D-Dimer antibody (Anti-DD) was immobilized on SPCE/LMA-AgNP. Along with the analytical characterization of the SPCE/LMA-AgNP/Anti-DD immunosensor, the linear range for DD, limit of detection (LOD), potential chemical interferences, and real sample applications were also evaluated. The antigen/antibody interaction at various DD concentrations was monitored using DPV. The SPCE/LMA-AgNP/Anti-DD shows a linear response in the DD concentration range of 0.01–1 pg/mL and a LOD of 0.2 fg/mL. In addition, the SPCE/LMA-AgNP/Anti-DD showed excellent specificity and no response to potential interfering substances, for instance urea, insulin, C-reactive protein (CRP), and serum amyloid A (SAA). Eventually, the SPCE/LMA-AgNP/Anti-DD is applied in human serum, which shows good recovery values (97.2 %). The SPCE/LMA-AgNP/Anti-DD can be fabricated cheaply and easily for bedside clinical evaluation.

d -二聚体（DD）是凝血和纤溶激活的关键标志物。临床医生可以在快速分析和量化的帮助下做出重要的决定，最好是在病人的床边。电化学免疫传感器是解决这些问题的有效护理点技术。本文描述了一种简单的用于检测DD的电化学免疫传感器的开发。该免疫传感器是通过在丝网印刷碳电极（SPCE）上电沉积乳糖甲氧基苯胺银纳米粒子（LMA-AgNPs）构建的。采用傅里叶变换红外光谱（FTIR）、高分辨率透射电子显微镜（HR-TEM）、扫描电子显微镜-能量色散x射线能谱（SEM-EDS）和x射线光电子能谱（XPS）对LMA-AgNP和SPCE/LMA-AgNP进行表征。采用电化学阻抗谱（EIS）、差分脉冲伏安法（DPV）和循环伏安法（CV）对SPCE/LMA-AgNP的电化学行为进行了表征。然后，将anti - d -二聚体抗体（Anti-DD）固定在SPCE/LMA-AgNP上。随着SPCE/LMA-AgNP/Anti-DD免疫传感器的分析表征，DD的线性范围，检测限（LOD），潜在的化学干扰和实际样品应用也进行了评估。采用DPV法监测不同DD浓度下抗原/抗体相互作用。SPCE/LMA-AgNP/Anti-DD在0.01-1 pg/mL的浓度范围内呈线性响应，LOD为0.2 fg/mL。此外，SPCE/LMA-AgNP/Anti-DD对尿素、胰岛素、c反应蛋白（CRP）、血清淀粉样蛋白A （SAA）等潜在干扰物质均无反应，具有良好的特异性。最终将SPCE/LMA-AgNP/Anti-DD应用于人血清，回收率为97.2%。SPCE/LMA-AgNP/Anti-DD制备成本低，易于临床评价。

{"title":"Development of a sugar-derived silver nanoparticle-based electrochemical immunosensor for sensitive D-dimer detection","authors":"Fatma Ozturk Kirbay , İdris Yazgan , Dilek Odaci","doi":"10.1016/j.sintl.2025.100332","DOIUrl":"10.1016/j.sintl.2025.100332","url":null,"abstract":"<div><div>D-dimer (DD) is a key marker of coagulation and fibrinolysis activation. Clinicians can make important decisions with the aid of quick analysis and quantification, ideally at the patient's bedside. Electrochemical immunosensors are effective point-of-care technologies to address these issues. Here, we describe the development of a simple electrochemical immunosensor to detect DD. The immunosensor is constructed by electrodeposition of lactose methoxide aniline silver nanoparticles (LMA-AgNPs) on a screen-printed carbon electrode (SPCE). The LMA-AgNP and SPCE/LMA-AgNP were characterized by Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of the SPCE/LMA-AgNP was examined using electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Then, Anti-D-Dimer antibody (Anti-DD) was immobilized on SPCE/LMA-AgNP. Along with the analytical characterization of the SPCE/LMA-AgNP/Anti-DD immunosensor, the linear range for DD, limit of detection (LOD), potential chemical interferences, and real sample applications were also evaluated. The antigen/antibody interaction at various DD concentrations was monitored using DPV. The SPCE/LMA-AgNP/Anti-DD shows a linear response in the DD concentration range of 0.01–1 pg/mL and a LOD of 0.2 fg/mL. In addition, the SPCE/LMA-AgNP/Anti-DD showed excellent specificity and no response to potential interfering substances, for instance urea, insulin, C-reactive protein (CRP), and serum amyloid A (SAA). Eventually, the SPCE/LMA-AgNP/Anti-DD is applied in human serum, which shows good recovery values (97.2 %). The SPCE/LMA-AgNP/Anti-DD can be fabricated cheaply and easily for bedside clinical evaluation.</div></div>","PeriodicalId":21733,"journal":{"name":"Sensors International","volume":"6 ","pages":"Article 100332"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of nickel incorporation on the physicochemical properties and performance of HKUST-1-based MOF immunosensor for DENV-3 NS1 detection 镍掺入对基于hkust -1的DENV-3 NS1检测MOF免疫传感器理化性质和性能的影响

Sensors International

Pub Date : 2025-01-01 DOI: 10.1016/j.sintl.2025.100331

Neng Astri Lidiawati , Muhammad Fadlan Raihan , Angga Hermawan , Octia Floweri , Robeth Viktoria Manurung , Muhammad Iqbal , Ahmad Nuruddin , Nugraha , Brian Yuliarto , Ni Luh Wulan Septiani

An electrochemical immunosensor was developed using a modified metal-organic framework (MOF) for detecting dengue virus serotype 3 (DENV-3) NS1. CuNi-MOF frameworks with varying Cu:Ni molar ratios—1:0 (CuNi-1), 5:1 (CuNi-2), 3:1 (CuNi-3), and 1:1 (CuNi-4)—were synthesized, incorporating 10 wt% triethanolamine (TEOA) as a modulator to enhance performance. X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectra confirm the successful formation of CuNi-MOF. Morphological evolution was observed as the Ni content increased to 50 % (CuNi-4), resulting in a transformation from octahedral to bar-like particles. Among the synthesized materials, CuNi-4 exhibited the highest redox current, making it the optimal choice for electrochemical measurements targeting dengue virus (DENV) detection. Using DPV technique, it was found that linear range of DENV-3 antigen concentration is 0.001 ng mL⁻¹ - 5 ng mL⁻¹ with a limit of detection (LoD) of 0.1 pg mL⁻¹. In addition, the CuNi-4 exhibited remarkable selectivity for DENV-3 over the other three dengue virus serotypes. Real sample testing using DENV-3 antigen with variation concentration of 0.001; 0.01; 0.1; 1; and 10 ng mL⁻¹ in commercial human serum resulted in the recovery of 98 %, 94 %, 97 %, 95 %, and 98 %, respectively. The findings highlight the promise of the CuNi-4-based electrochemical immunosensor for detecting NS-1 antigens of DENV-3 with high specificity and sensitivity.

采用改良金属-有机骨架（MOF）研制了一种检测登革热病毒血清型3 (DENV-3) NS1的电化学免疫传感器。分别合成了Cu:Ni摩尔比为1:0 （Cu -1）、5:1 （Cu -2）、3:1 （Cu -3）和1:1 （Cu -4）的Cu - mof框架，并添加了10%的三乙醇胺（TEOA）作为调制剂来提高性能。x射线衍射（XRD）和傅里叶变换红外光谱（FTIR）证实了cu - mof的成功形成。当Ni含量增加到50% （cu -4）时，观察到形态进化，导致八面体颗粒转变为条状颗粒。在合成的材料中，cu -4表现出最高的氧化还原电流，使其成为针对登革热病毒（DENV）检测的电化学测量的最佳选择。DPV技术检测结果显示，DENV-3抗原浓度线性范围为0.001 ng mL−1 ~ 5 ng mL−1，检出限为0.1 pg mL−1。此外，CuNi-4对DENV-3的选择性优于其他3种登革热病毒血清型。用变异浓度为0.001的DENV-3抗原检测实样；0.01;0.1;1;10 ng mL−1的回收率分别为98%、94%、97%、95%和98%。这些发现突出了基于cu -4的电化学免疫传感器对DENV-3 NS-1抗原的高特异性和高灵敏度检测的前景。

{"title":"Effect of nickel incorporation on the physicochemical properties and performance of HKUST-1-based MOF immunosensor for DENV-3 NS1 detection","authors":"Neng Astri Lidiawati , Muhammad Fadlan Raihan , Angga Hermawan , Octia Floweri , Robeth Viktoria Manurung , Muhammad Iqbal , Ahmad Nuruddin , Nugraha , Brian Yuliarto , Ni Luh Wulan Septiani","doi":"10.1016/j.sintl.2025.100331","DOIUrl":"10.1016/j.sintl.2025.100331","url":null,"abstract":"<div><div>An electrochemical immunosensor was developed using a modified metal-organic framework (MOF) for detecting dengue virus serotype 3 (DENV-3) NS1. CuNi-MOF frameworks with varying Cu:Ni molar ratios—1:0 (CuNi-1), 5:1 (CuNi-2), 3:1 (CuNi-3), and 1:1 (CuNi-4)—were synthesized, incorporating 10 wt% triethanolamine (TEOA) as a modulator to enhance performance. X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectra confirm the successful formation of CuNi-MOF. Morphological evolution was observed as the Ni content increased to 50 % (CuNi-4), resulting in a transformation from octahedral to bar-like particles. Among the synthesized materials, CuNi-4 exhibited the highest redox current, making it the optimal choice for electrochemical measurements targeting dengue virus (DENV) detection. Using DPV technique, it was found that linear range of DENV-3 antigen concentration is 0.001 ng mL<sup>−1</sup> - 5 ng mL<sup>−1</sup> with a limit of detection (LoD) of 0.1 pg mL<sup>−1</sup>. In addition, the CuNi-4 exhibited remarkable selectivity for DENV-3 over the other three dengue virus serotypes. Real sample testing using DENV-3 antigen with variation concentration of 0.001; 0.01; 0.1; 1; and 10 ng mL<sup>−</sup><sup>1</sup> in commercial human serum resulted in the recovery of 98 %, 94 %, 97 %, 95 %, and 98 %, respectively. The findings highlight the promise of the CuNi-4-based electrochemical immunosensor for detecting NS-1 antigens of DENV-3 with high specificity and sensitivity.</div></div>","PeriodicalId":21733,"journal":{"name":"Sensors International","volume":"6 ","pages":"Article 100331"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast micrometer resolution magneto-optical viscosity measurements with hard magnetic nanoplatelets 快速微米分辨率磁光粘度测量与硬磁性纳米片

Sensors International

Pub Date : 2025-01-01 DOI: 10.1016/j.sintl.2025.100337

Artem Eliseev , Vladimir Korolev , Evgeny Anokhin , Andrei Chumakov , Alexander Vasiliev , Lev Trusov , Pavel Kazin , Andrei Eliseev

The paper reports on the application of hard magnetic nanoplatelets for direct local dynamic viscosity evaluation in liquids through their drag-hindered rotation after a magnetic field. Strontium hexaferrite, SrFe₁₂O₁₉ nanoplatelets with a diameter of 50 nm and a thickness of 5 nm were employed as nanoscale probes. The dynamics of nanoplatelets is investigated with AC magnetometry, small-angle X-ray scattering, and optical absorption/reflectance spectroscopy. Those reveal well-defined relation of drag force and phase lag tangent for platelets rotation under AC magnetization with the viscosity of the medium. The analytical quantitative description for magneto-optical response is provided and utilized successfully for independent viscosity evaluation of various liquids within the viscosity range of 0.5–1000 mPa∙s. The nanoscale size of the probes also enabled viscosity visualization with micrometer resolution in microchannels.

本文报道了硬磁性纳米片在磁场作用下通过阻阻旋转直接评价液体局部动态粘度的应用。采用直径为50 nm、厚度为5 nm的六铁体锶、SrFe12O19纳米薄片作为纳米探针。用交流磁强计、小角x射线散射和光学吸收/反射光谱法研究了纳米血小板的动力学。这些结果表明，在交流磁化条件下，血小板旋转的阻力和相位滞后切线与介质粘度有明确的关系。提供了磁光响应的分析定量描述，并成功地用于粘度范围为0.5-1000 mPa∙s的各种液体的独立粘度评估。探针的纳米级尺寸还可以在微通道中以微米级分辨率显示粘度。

引用次数: 0

Sensors International

Pub Date : 2025-01-01

引用次数: 0

Sensors International

Pub Date : 2025-01-01

引用次数: 0

Sensors International

Pub Date : 2025-01-01

引用次数: 0

Sensors International

Pub Date : 2025-01-01

引用次数: 0

Sensors International

Pub Date : 2025-01-01

引用次数: 0

Sensors International

Pub Date : 2025-01-01

引用次数: 0

首页上一页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Sensors International

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀