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DYNAMICS OF ARGALI POPULATION (OVIS AMMON LINNAEUS, 1758) IN KAZAKHSTAN 哈萨克斯坦盘羊种群动态(OVIS AMMON LINNAEUS,1758)
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-02 DOI: 10.12731/2658-6649-2022-14-4-171-184
V. Salovarov, Daniyar N. Yesmukhanbetov, Zhaskaiyr M. Karagoishin
The research materials are based on the processing and analyzing the official results of the air and ground surveys conducted in 2019, providing departmental materials. The habitats with a high number of mountain sheep include the Karaganda, Turkestan, Dzhambul and Almaty regions of the Republic of Kazakhstan. The paper aims to estimate the changes in argali’s number and reveal the basic influencing factors. The total number of wild mountain sheep in Kazakhstan is 17954 heads. The paper shows that the share of the influence of environmental and anthropogenic factors on the number of argalis is 99.7%. The main influence on the number of snow sheep is exerted by anthropogenic factors such as poaching and economic use of the range of wild mountain sheep by humans. From time to time, severe fires affect the deterioration of the habitat. The authors attribute the creation of specially protected natural territories and the inclusion of subspecies in the Red Book of Kazakhstan to the positive impact of human activity on the argali population. In modern conditions, the natural potential viability and survival of mountain sheep and further naturalization are weak, although there is an increase in the number of argalis. These aspects indicate that the anthropogenic factor affects animals intensely. The analysis of variance shows and testifies to the high influence of the anthropogenic factor on the number of argalis in regions of Kazakhstan. Today, to improve the protection of mountain sheep, it is advisable to organize a network of specially protected natural territories in the mountainous regions of the south and south-east of Kazakhstan.
研究材料基于对2019年进行的空中和地面调查的官方结果的处理和分析,提供了部门材料。山区绵羊数量较多的栖息地包括哈萨克斯坦共和国的卡拉干达、突厥斯坦、扎姆布尔和阿拉木图地区。本文旨在估计盘羊数量的变化,揭示影响盘羊数量变化的基本因素。哈萨克斯坦的野生山羊总数为17954头。研究表明,环境和人为因素对银羊数量的影响比例为99.7%,对雪羊数量的主要影响是人为因素,如偷猎和人类对野生山羊范围的经济利用。严重的火灾不时影响栖息地的恶化。作者将创建特别保护的自然领土和将亚种列入哈萨克斯坦红皮书归因于人类活动对盘羊种群的积极影响。在现代条件下,山羊的自然潜在生存能力和生存能力以及进一步的归化能力较弱,尽管argalis的数量有所增加。这些方面表明,人为因素对动物的影响很大。方差分析表明并证明了人为因素对哈萨克斯坦地区argalis数量的高度影响。今天,为了加强对山羊的保护,最好在哈萨克斯坦南部和东南部山区组织一个特别保护的自然领地网络。
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引用次数: 0
NEW PROMISING AGENTS FOR PHOTODYNAMIC THERAPY 有前途的光动力治疗新药物
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.12731/2658-6649-2022-14-4-11-22
O. V. Shevchenko, N. G. Plekhova, O. Korshunova, I. Tananaev, V. Apanasevich
The problem of oncological diseases has acquired a social and medical character. In this regard, the rapid development of new drugs and methods, as well as modification of existing methods in order to increase their effectiveness, is currently required. The paper aims to develop and study two promising agents for photodynamic therapy. We discussed a new binary complex based on the widely used photosensitizer chlorin E6 with europium, which can solve one of the main disadvantages of drugs for photodynamic therapy: it can be used to treat deeper tumors and act more effectively by transferring luminescence energy between europium and chlorin E6. Our other agent was copper-containing chlorophyllin. Chlorophyllin is a natural component, which is always promising in the development of new drugs. The optical and fluorescent parameters of the agents were determined using physicochemical methods; their ability to generate reactive oxygen species in the presence of two fluorescent probes was studied: 2ʹ, 7ʹ-dichlorodihydrofluorescein and dihydrofluorescein. Thus, we showed the effectiveness of chlorophyllin in comparison with chlorin E6 by 1.92 times at 50 μg / ml E6 and showed the effectiveness of the binary complex even at the minimum concentration of 10 μg / ml. Further research in cell cultures will develop the ways of the possible use of new substances as photosensitizers.
肿瘤学疾病的问题具有社会性和医学性。在这方面,目前需要迅速开发新的药物和方法,并修改现有方法以提高其有效性。本文旨在开发和研究两种有前景的光动力治疗剂。我们讨论了一种基于广泛使用的光敏剂氯化氢E6和铕的新的二元络合物,它可以解决光动力治疗药物的主要缺点之一:它可以用于治疗更深层次的肿瘤,并通过在铕和氯化氢E6之间转移发光能而更有效地发挥作用。我们的另一种药剂是含铜叶绿素。叶绿素是一种天然成分,在新药开发中一直具有广阔的前景。采用物理化学方法测定了制剂的光学和荧光参数;研究了它们在2,7-二氯二氢荧光素和二氢荧光素两种荧光探针存在下产生活性氧的能力。因此,我们在50μg/ml E6下显示了叶绿素与绿泥石E6相比的1.92倍的有效性,并且即使在最低浓度为10μg/ml的情况下也显示了二元复合物的有效性。对细胞培养的进一步研究将开发新物质作为光敏剂的可能用途。
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引用次数: 0
PALEOECOLOGICAL CONDITIONS AND CARBON ACCUMULATION IN GENESIS OF INUNDATED MIRE OF MID-RUSSIAN UPLAND 俄罗斯中部高原淹没沼泽成因的古生态条件与碳积累
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-70-91
E. Volkova, O. Leonova, Vasyliy V. Mironov
The aim is to study of influence of the ecological conditions on the carbon accumulation at the different stages of genesis of the inundated mire. Materials and methods. The macrofossil analysis and the degree of peat decomposition were studied by the microscopic method, the moisture content and the dry bulk density were studied by the gravimetric method. The ash content was determined by burning, the content of organic matter was calculated by the difference between the mass of dry peat and the ash content. The carbon content (%) in peat samples was calculated based on the dry bulk density, the organic matter content and the mass fraction of carbon for each volume of peat. The rate of vertical growth of peat and intensity of peat accumulation in periods of Holocene were calculated based on the results of radioactive dating. The parameters of surrounding landscapes during the mire genesis were characterized by the results of the palynology analysis and the estimation of fire activity. The intensity of fires in paleolandscapes was studied by the content of coal particles in each (1 cm3) sample of wet peat. Results. The genesis of the Podkosmovo mire is presented by 5 stages which were took place under the stable conditions of water-mineral nutrition. The carbon accumulation in the genesis proceeded at the rate of 45.7 g/m2/year on average. At the same time, the surrounding landscapes differed in the degree of afforestation and the intensity of anthropogenic press. Conclusion. The moderate hydration and nutrition with mineralized waters ensure the stability of the mire development and the intensity of carbon accumulation, despite the change of surrounding landscapes under the influence of climate or human. However, the active transformation of the surrounding landscapes can have a negative impact on the functioning of the mire and reduce the intensity of carbon accumulation.
目的是研究生态条件对淹没淤泥形成不同阶段碳积累的影响。材料和方法。用显微方法对泥炭的宏观化石分析和分解程度进行了研究,用重量法对泥炭的含水率和干容重进行了研究。通过燃烧测定灰分,通过干泥炭质量和灰分之间的差异计算有机物含量。泥炭样品中的碳含量(%)是根据干容重、有机物含量和每体积泥炭的碳质量分数计算的。根据放射性测年结果,计算了全新世泥炭的垂直生长速率和泥炭堆积强度。根据孢粉学分析和火灾活动性估算结果,对泥沼形成过程中周围景观的参数进行了表征。通过每个(1 cm3)湿泥炭样品中的煤颗粒含量来研究古景观中的火灾强度。后果Podkosmovo泥沼的形成分为5个阶段,它们发生在水矿物营养稳定的条件下。碳在成因中的积累速率平均为45.7克/平方米/年。同时,周边景观在造林程度和人为压力强度上存在差异。结论矿化水的适度水合和营养确保了沼泽发育的稳定性和碳积累的强度,尽管周围景观在气候或人类的影响下发生了变化。然而,对周围景观的积极改造会对沼泽的功能产生负面影响,并降低碳积累的强度。
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引用次数: 0
ISOLATION OF METHANOTROPHIC BACTERIA FROM NATURAL BIOCENOSES AND SELECTION OF NUTRIENT MEDIA OPTIMAL FOR CULTIVATION IN LABORATORY CONDITIONS 天然生物中甲烷营养菌的分离及培养基的筛选
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-107-121
O. V. Prokudina, Georgiy V. Pestsov, Anastasia Tretyakova
Background. One of the most pressing problems in the world is the production of food protein. The global protein deficit is estimated at 30 million tons per year, for the Russian Federation it is estimated at 2-2.5 million tons of protein per year. The problem of protein deficit can be partially solved with the help of methanotrophic bacteria. Under optimal conditions, methanotrophic bacteria are able to oxidise actively natural gas – methane and increase their biomass, rich in valuable protein, vitamins and other biologically active substances. The feed product from methanotrophic bacteria is comparable to fish meal and soybean meal, the concentration of pure protein in it is more than 80%. Purpose. Isolation of methanotrophic bacteria from natural biocenoses and their cultivation on various nutrient media in laboratory conditions. Materials and Methods. The study was conducted from 2019 to 2022 in the microbiological laboratory of the Center for Technological Excellence “Advanced Chemical and Biotechnology” of the Tolstoy Tula State Pedagogical University. The object of the study was methanotrophic bacteria isolated from various natural and artificial ecosystems, collection strains of Methylococcus capsulatus B-2990 and Methylomonas methanica B-2110. To isolate methanotrophic bacteria, the method of seeding a accumulative culture on a solid agiarized sterile nutrient medium in Petri dishes was used. For the cultivation of methanotrophs, nutrient media of various compositions containing all the necessary macro- and microelements for the normal growth of bacterial colonies were used. Results. Colonies of methanotrophic bacteria were found in one sample of bottom sediments taken from the sphagnum swamp of the village of Kochaki in the Tula region and in samples from a methane reactor. As a result of comparison of nutrient media for isolation and cultivation of methanotrophs, it was found that the most suitable medium for isolation of methanotrophic bacteria is the standard nitrate mineral medium NMS. For the cultivation of methanotrophic bacteria, the best media were: the nutrient medium with the addition of anaerobic sludge, the ammonium mineral nutrient medium (AMS), and AMS with addition of methanol, the nutrient medium No.221 with addition of methanol. Conclusions. The use of various nutrient media for the cultivation of methanotrophic bacteria makes it possible to assess the effect of various organic substances on the growth and development of bacterial colonies. The study of various natural and artificial ecosystems makes it possible to expand taxonomic knowledge about this group of microorganisms, as well as to increase the potential of using methanotrophs in solving various problems of ecology and biotechnology.
背景。世界上最紧迫的问题之一是食品蛋白质的生产。全球蛋白质赤字估计为每年3 000万吨,俄罗斯联邦的蛋白质赤字估计为每年200 - 250万吨。利用甲烷营养菌可以部分解决蛋白质缺乏的问题。在最佳条件下,甲烷营养细菌能够有效氧化天然气甲烷,增加其生物量,富含有价值的蛋白质、维生素和其他生物活性物质。产甲烷营养菌的饲料产品与鱼粉、豆粕相当,其中纯蛋白质含量达80%以上。目的。天然海藻中甲烷营养细菌的分离及其在不同营养培养基上的培养。材料与方法。该研究于2019年至2022年在托尔斯泰图拉国立师范大学“先进化学与生物技术”卓越技术中心的微生物实验室进行。研究对象为从各种自然生态系统和人工生态系统中分离得到的甲烷营养细菌,收集菌株荚膜甲基球菌B-2990和甲烷甲基单胞菌B-2110。为了分离甲烷营养细菌,采用了在培养皿中固体无菌营养培养基上播种累积培养的方法。为了培养甲烷氧化菌,使用了含有细菌菌落正常生长所需的所有宏量和微量元素的各种成分的营养培养基。结果。在图拉地区Kochaki村泥炭沼泽的底部沉积物样本和甲烷反应器样本中发现了甲烷营养细菌菌落。通过对分离和培养甲烷氧化菌的营养培养基进行比较,发现最适合分离甲烷氧化菌的培养基为标准硝酸盐矿物培养基NMS。对于甲烷营养菌的培养,最佳培养基为:添加厌氧污泥的营养培养基、铵矿物营养培养基(AMS)、添加甲醇的AMS、添加甲醇的221号营养培养基。结论。利用各种营养培养基进行甲烷营养菌的培养,可以评估各种有机物对菌落生长发育的影响。通过对各种自然和人工生态系统的研究,可以扩大对这类微生物的分类知识,并增加利用甲烷氧化菌解决各种生态学和生物技术问题的潜力。
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引用次数: 0
THE ISSUES OF CHARACTER ACCENTUATION ADOLESCENTS WITH DIABETES 糖尿病青少年的性格突出问题
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-172-190
S. Kolkova, Yulia V. Zhivaeva, Ekaterina I. Stoyanova, T. Kazakova, V. R. Kembel, T. A. Guseva
Purpose. Theoretical justification and empirical study of the characteristics of adolescents’ personality with diabetes mellitus Method. The study was conducted on a sample of 40 adolescents (20 with diabetes and 20 without). To determine the character accentuations, the following were used: Leonhard’s questionnaire and the method of identification characterological verbal portraits – E.G. Eidemiller. To compare the groups, correlation analysis was used (Mann-Whitney U test). Results. Within group of adolescents with diabetes, the sensitive (40%) and schizoid (30%) types of accentuation prevail, while in healthy adolescents, the hyperthymic (55%) type of accentuation prevails. Adolescents with diabetes were more conflicted and less capable of self-control than a group of adolescents without a diagnosis. On average, accentuations were more pronounced in healthy adolescents, the average scores for each of the accentuations in patients with diabetes mellitus were lower. Also, the correlation coefficients between the indicators of the K. Leonhard method and the indicators of the E.G. Eidemiller method on samples of adolescents with and without a diagnosis of diabetes mellitus turned out to be different. In the group of adolescents with diabetes mellitus, correlations were observed between the following parameters: “emotive type” and “neurasthenic type” (r=0.81, p=0.05), “anxious type” and “paranoid type” (r=0.65, p=0.05), “excitable type” and “unstable type” (r=0.65, p=0.05). In a group of healthy adolescents, the following correlations were found between the indicators of the methods of K. Leonhard and E. G. Eidemiller: “pedantic type” and “hyperthymic type” (r=0.63, p=0.05), “exalted type” and “paranoid type” (r=0.65, p=0.05), “pedantic type” and “hysterical type” (r=0.70, p=0.05), “conformal type” (r=0.63, p=0.05), “cyclothymic type” and “conformal type” (r=0.67, p=0.05). Conclusion. Such results will allow psychologists to more accurately take into account the personal characteristics of adolescents with diabetes and provide them with more effective assistance. Targets for work with adolescent with diabetes mellitus can be their introversion and, accordingly, assiste them in social adaptation, help with lack of self-control, aggressiveness.
目的。糖尿病青少年人格特征的理论论证与实证研究这项研究以40名青少年为样本进行,其中20名患有糖尿病,20名没有糖尿病。为了确定角色的重音,使用了以下方法:莱昂哈德的问卷和识别特征语言肖像的方法-例如埃德米勒。组间比较采用相关分析(Mann-Whitney U检验)。结果。在青少年糖尿病患者群体中,以敏感型(40%)和精神分裂型(30%)加重为主,而在健康青少年中,以胸腺肥大型(55%)加重为主。与未确诊的青少年相比,患有糖尿病的青少年更加矛盾,自我控制能力也更差。平均而言,健康青少年的口音更为明显,糖尿病患者的每种口音的平均得分较低。此外,K. Leonhard方法的指标与eg . Eidemiller方法的指标在诊断为糖尿病和未诊断为糖尿病的青少年样本中的相关系数也有所不同。在青少年糖尿病组中,“情绪型”与“神经衰弱型”(r=0.81, p=0.05)、“焦虑型”与“偏执型”(r=0.65, p=0.05)、“兴奋型”与“不稳定型”(r=0.65, p=0.05)存在相关性。在健康青少年群体中,K. Leonhard和E. G. Eidemiller方法的指标:“迂腐型”与“胸腺亢进型”(r=0.63, p=0.05)、“亢进型”与“偏执型”(r=0.65, p=0.05)、“迂腐型”与“歇斯底里型”(r=0.70, p=0.05)、“适形型”(r=0.63, p=0.05)、“循环胸腺型”与“适形型”(r=0.67, p=0.05)之间存在相关性。结论。这样的结果将使心理学家能够更准确地考虑到青少年糖尿病患者的个人特征,并为他们提供更有效的帮助。青少年糖尿病患者的工作目标可以是他们的内向,因此,帮助他们适应社会,帮助他们缺乏自我控制,侵略性。
{"title":"THE ISSUES OF CHARACTER ACCENTUATION ADOLESCENTS WITH DIABETES","authors":"S. Kolkova, Yulia V. Zhivaeva, Ekaterina I. Stoyanova, T. Kazakova, V. R. Kembel, T. A. Guseva","doi":"10.12731/2658-6649-2022-14-6-172-190","DOIUrl":"https://doi.org/10.12731/2658-6649-2022-14-6-172-190","url":null,"abstract":"Purpose. Theoretical justification and empirical study of the characteristics of adolescents’ personality with diabetes mellitus \u0000Method. The study was conducted on a sample of 40 adolescents (20 with diabetes and 20 without). To determine the character accentuations, the following were used: Leonhard’s questionnaire and the method of identification characterological verbal portraits – E.G. Eidemiller. To compare the groups, correlation analysis was used (Mann-Whitney U test). \u0000Results. Within group of adolescents with diabetes, the sensitive (40%) and schizoid (30%) types of accentuation prevail, while in healthy adolescents, the hyperthymic (55%) type of accentuation prevails. Adolescents with diabetes were more conflicted and less capable of self-control than a group of adolescents without a diagnosis. On average, accentuations were more pronounced in healthy adolescents, the average scores for each of the accentuations in patients with diabetes mellitus were lower. Also, the correlation coefficients between the indicators of the K. Leonhard method and the indicators of the E.G. Eidemiller method on samples of adolescents with and without a diagnosis of diabetes mellitus turned out to be different. In the group of adolescents with diabetes mellitus, correlations were observed between the following parameters: “emotive type” and “neurasthenic type” (r=0.81, p=0.05), “anxious type” and “paranoid type” (r=0.65, p=0.05), “excitable type” and “unstable type” (r=0.65, p=0.05). In a group of healthy adolescents, the following correlations were found between the indicators of the methods of K. Leonhard and E. G. Eidemiller: “pedantic type” and “hyperthymic type” (r=0.63, p=0.05), “exalted type” and “paranoid type” (r=0.65, p=0.05), “pedantic type” and “hysterical type” (r=0.70, p=0.05), “conformal type” (r=0.63, p=0.05), “cyclothymic type” and “conformal type” (r=0.67, p=0.05). \u0000Conclusion. Such results will allow psychologists to more accurately take into account the personal characteristics of adolescents with diabetes and provide them with more effective assistance. Targets for work with adolescent with diabetes mellitus can be their introversion and, accordingly, assiste them in social adaptation, help with lack of self-control, aggressiveness.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46007529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AGRICULTURAL FINANCING AS THE BASIS FOR FOOD SECURITY IN KAZAKHSTAN UNDER EAEU CONDITIONS 欧盟条件下哈萨克斯坦粮食安全的农业融资基础
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-404-422
Yerlan A. Alibayev, M. Onuchko
With Kazakhstan’s accession to the Eurasian Economic Union, the government needed to change the instruments to support agriculture and the agro-industrial complex as a whole. As it is on its success that the provision and condition of food security of the country directly depends. Food security is very urgent, especially during the current financial crisis. To date, the instability of agriculture in Kazakhstan is the main reason for the low level of food security in the country. One of the strategic tasks for Kazakhstan is to ensure food security. There is a lack of scientific research aimed at financing and state support of agriculture as an important prerequisite for ensuring food security in Kazakhstan under the conditions of the Eurasian Economic Union, which determines the purpose of our study. The following research methods were used in the work: economic and statistical; economic and mathematical methods, including methods of statistical analysis. We use correlation and regression analysis to study the relationship between public financing of the agro-industrial complex and food security. By this we not only analyze the current situation, but also make it possible to implement a medium-term forecast. The result of the study is a structural analysis of the financing of the agro-industrial complex, which shows that the bulk of the financing is carried out by own funds, and the amount of public investment is less than own resources. Based on the study, the dependence of agriculture in Kazakhstan under the EAEU conditions on state funding and support has been revealed. The main ways and solutions to ensure food security of Kazakhstan in the conditions of the Eurasian Economic Union have been proposed. Conclusions. Ensuring food security of the population is an integral part of economic, political and national security of the country. In this context, the agro-industrial complex is the main key factor and epicentre of food security of the country, and without state financial support for agriculture, food security of Kazakhstan is and will be under threat. Only by solving this urgent problem can Kazakhstan lead among the member countries of the Eurasian Economic Union. Therefore, in order to ensure food security, measures that need to be taken to develop a food security policy have been proposed. In addition, proposals have been made to regulate and control food security in Kazakhstan.
随着哈萨克斯坦加入欧亚经济联盟,政府需要改变支持农业和整个农产工业综合体的手段。该国粮食安全的提供和状况直接取决于它的成功。粮食安全非常紧迫,特别是在当前金融危机期间。迄今为止,哈萨克斯坦农业的不稳定是该国粮食安全水平低的主要原因。哈萨克斯坦的战略任务之一是确保粮食安全。缺乏旨在资助和国家支持农业的科学研究,这是在欧亚经济联盟的条件下确保哈萨克斯坦粮食安全的重要先决条件,这决定了我们研究的目的。工作中采用了以下研究方法:经济学和统计学;经济和数学方法,包括统计分析方法。运用相关和回归分析方法,研究了农工综合体公共融资与粮食安全的关系。通过这一点,我们不仅可以分析当前的形势,而且可以实现中期预测。研究结果是对农工综合体融资的结构分析,表明大部分融资由自有资金进行,公共投资额低于自有资源。根据这项研究,揭示了在EAEU条件下哈萨克斯坦农业对国家资金和支持的依赖性。提出了在欧亚经济联盟条件下确保哈萨克斯坦粮食安全的主要途径和解决方案。结论。确保人民的粮食安全是国家经济、政治和国家安全的组成部分。在这种情况下,农工业综合体是该国粮食安全的主要关键因素和中心,如果没有国家对农业的财政支持,哈萨克斯坦的粮食安全现在和将来都将受到威胁。只有解决这一紧迫问题,哈萨克斯坦才能在欧亚经济联盟成员国中处于领先地位。因此,为了确保粮食安全,提出了制定粮食安全政策所需采取的措施。此外,还提出了规范和控制哈萨克斯坦粮食安全的建议。
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引用次数: 0
STUDY OF THE EFFECT OF NEW 6-R-3,5-DINITRO-1,2,3,4-TETRAHYDROPYRIDINES ON THE ACCUMULATION OF PHOTOSYNTHETIC PIGMENTS AND ASCORBIC ACID IN PLANT TISSUES 新型6-R-3,5-二硝基-1,2,3,4-四氢吡啶对植物组织光合色素和抗坏血酸积累影响的研究
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-372-387
E. V. Ivanova, Nikolay Zhukov, I. Surova, M. Nikishina, L. Mukhtorov, Yurij Atroshchenko
Background. The problem of finding new effective plant protection products remains an urgent task of modern agrochemistry. It is known that tetrahydropyridine derivatives exhibit a broad spectrum of biological activity, including fungicidal activity. However, compounds with potential biological activity can also be highly toxic to humans, cultivated crops, and the environment as a whole, so it is necessary to study their environmental safety. The content of photosynthetic pigments and ascorbic acid are one of the most important biochemical indicators of plant response to environmental stress factors, therefore, determining their amount helps to assessthe physiological state of plants and analyze the toxicity of the synthesized compounds. Purpose. To study the effect of new 6-R-3,5-dinitro-1,2,3,4-tetrahydropyridines on the content of photosynthetic pigments and ascorbic acid in plant tissues in order to assess the safety of their use as potential plant protection products. Materials and methods. The test compounds were obtained by the previously developed method for the selective reduction of 2-R-3,5-dinitropyridines. Winter wheat seeds of the Moskovskaya 39 variety were germinated in Petri dishes using solutions of the synthesized substances. Seeds germinated in water were used as controls. On the ninth day, the content of various groups of photosynthetic pigments (chlorophylls a, b, carotenoids), as well as ascorbic acid in plant tissues was determined. All experiments were carried out in triplicate. In addition, the analytes were tested for toxicity using the GUSAR Environmental Toxicity software. Results. The effect of new derivatives of tetrahydropyridine on the accumulation of photosynthetic pigments and ascorbic acid in plant tissues of winter wheat was studied. It was shown that the studied compounds do not have a significant toxic effect on plants. Conclusion. As a result of studying the effect of new 6-R-3,5-dinitro-1,2,3,4-tetrahydropyridines on the content of photosynthetic pigments and ascorbic acid, as well as in silico computer prediction in GUSAR Environmental Toxicity, it was found that, in general, the studied compounds are relatively safe and, therefore, promising for further testing as biologically active plant protection products.
背景寻找新的有效植物保护产品仍然是现代农业化学的一项紧迫任务。众所周知,四氢吡啶衍生物具有广泛的生物活性,包括杀菌活性。然而,具有潜在生物活性的化合物也可能对人类、种植作物和整个环境具有高度毒性,因此有必要研究其环境安全性。光合色素和抗坏血酸的含量是植物对环境胁迫因子反应的最重要的生化指标之一,因此,测定它们的含量有助于评估植物的生理状态和分析合成化合物的毒性。意图研究新型6-R-3,5-二硝基-1,2,3,4-四氢吡啶对植物组织中光合色素和抗坏血酸含量的影响,以评估其作为潜在植物保护产品的安全性。材料和方法。试验化合物是通过先前开发的选择性还原2-R-3,5-二硝基吡啶的方法获得的。Moskovskaya 39品种的冬小麦种子在培养皿中使用合成物质的溶液发芽。在水中发芽的种子被用作对照。第九天,测定了植物组织中各种光合色素(叶绿素a、b、类胡萝卜素)以及抗坏血酸的含量。所有实验一式三份进行。此外,使用GUSAR环境毒性软件对分析物进行毒性测试。后果研究了新的四氢吡啶衍生物对冬小麦植物组织中光合色素和抗坏血酸积累的影响。研究表明,所研究的化合物对植物没有显著的毒性作用。结论通过研究新型6-R-3,5-二硝基-1,2,3,4-四氢吡啶对光合色素和抗坏血酸含量的影响,以及GUSAR环境毒性的计算机预测,发现所研究的化合物总体上是相对安全的,因此有望作为生物活性植物保护产品进一步测试。
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引用次数: 0
STUDY OF BIOLOGICAL ACTIVITY COLLOIDAL SOLUTIONS OF IRON SYNTHESIZED ON THE BASIS OF AQUEOUS CUFF EXTRACT 以袖带水提物为基础合成铁胶体溶液的生物活性研究
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-388-403
M. Nikishina, E. V. Ivanova, Anastasia Tretyakova, L. Mukhtorov, Yurij Atroshchenko
Background. Anthropogenic impact on agricultural areas leads to pollution of environmental objects, as well as to an increase in the resistance of pathogens to modern means of protection. One of the ways to solve these problems is the development of new drugs based on colloidal solutions of metals synthesized by the “green synthesis” method using aqueous extracts of medicinal plants from central Russia. The biological activity of metal nanoparticles obtained using plant extracts is currently being actively studied in relation to various biological objects. It is advisable to study the effect of iron colloids obtained with the help of an aqueous extract of the common mantle on the growth and development of wheat plants, as well as on phytopathogen fungi, the causative agents of the main diseases of agricultural plants. Purpose. To study the biological activity of iron colloids synthesized on the basis of aqueous extracts of the common cuff. To establish the effect of iron nanoparticles on the growth and development of wheat plants, and to study the fungicidal effect of colloids on pathogenic fungi. Materials and methods. Iron colloids were obtained from plant extracts of various dilutions and iron salt solution. The content of sugars, tannins and dyes, photosynthetic pigments, vegetable protein, flavonoids and ascorbic acid was determined in the composition of the common cuff. The influence of iron colloids on the growth and development of agricultural plants was studied by analyzing the seed germination energy and biometric parameters of wheat germs treated with initial extracts and synthesized colloids. The fungicidal activity of common mantle extracts and iron colloids synthesized on their basis was studied in vitro on the following fungi – phytopathogens: F. moniliforme and F. oxysporum, V. inaequalis, R. solani, B. sorokiniana, P. ostreatus and A. alternata. Results. Growth-stimulating activity is exhibited by aqueous extracts of the common cuff of an average degree of dilution and iron colloids synthesized on their basis. Only extracts of the common cuff have fungistatic properties in relation to the fungus B. sorokiniana. Conclusion. Biological activity is shown by extracts of medium dilution and colloids synthesized on their basis. In further research on this topic, it is advisable to study the effect of various variations in salt and extract concentrations on the manifestation of fungicidal and growth-stimulating activity.
背景。对农业地区的人为影响导致环境物体的污染,以及病原体对现代保护手段的抵抗力增加。解决这些问题的方法之一是开发基于“绿色合成”方法合成的金属胶体溶液的新药,这种方法使用来自俄罗斯中部的药用植物的水提取物。利用植物提取物获得的金属纳米颗粒的生物活性目前正在积极研究与各种生物物体有关的生物活性。研究用普通地膜水提物获得的铁胶体对小麦植株生长发育的影响,以及对植物病原真菌(农业植物主要病害的病原体)的影响是可取的。目的。研究以普通袖带水提物为基础合成的铁胶体的生物活性。建立铁纳米颗粒对小麦植株生长发育的影响,研究胶体对病原菌的杀真菌作用。材料和方法。从不同稀释度的植物提取物和铁盐溶液中获得铁胶体。测定了普通袖口中糖、单宁和染料、光合色素、植物蛋白、黄酮类化合物和抗坏血酸的含量。通过分析小麦胚芽经初始提取物和合成胶体处理后的种子发芽能和生物特征参数,研究铁胶体对农业植物生长发育的影响。在体外研究了以其为基础合成的常见地幔提取物和铁胶体对真菌和植物病原体的杀真菌活性,包括念珠菌和尖孢菌、不等分弧菌、番茄枯萎菌、白僵菌、绿僵菌和交替芽孢杆菌。结果。平均稀释度的普通袖带的水提取物和在其基础上合成的铁胶体显示出刺激生长的活性。只有普通袖草的提取物具有与sorokiniana真菌相关的抑菌特性。结论。生物活性表现为培养基稀释提取物和在其基础上合成的胶体。在本课题的进一步研究中,宜研究不同盐和提取物浓度对其杀菌和促生长活性表现的影响。
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引用次数: 0
DIGESTIBILITY AND ABSORPTION OF NUTRIENTS BY YOUNG CATTLE AT INCLUSION OF COMPLEX FEED ADDITIVES INTO THE DIETS IN THE CONDITIONS OF YAKUTIA 牦牛日粮中添加复合饲料添加剂对幼牛营养物质的消化吸收
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-321-337
M. Grigorev, V. Soloshenko, A. Grigoreva, N. Chernogradskaya, D. Stepanova
It is known that cattle with a high genetic potential of productivity cannot fully realize it in the conditions of Yakutia. This is facilitated by many factors, especially it is necessary to highlight the basic feeds, which are poorly provided with nutrients and minerals. Therefore, the development of technologies and methods to increase productivity of cattle through inclusion of complex feed additives from natural resources in their diets is of scientific and practical importance for the conditions of risky agriculture. In this regard, the goal was to study effect of complex feed additives from local natural raw materials on the digestibility and metabolism of young cattle. The studies used methods generally accepted in zootechnical practice, setting up for the experiment was carried out according to the method (M.F. Tomme, 1969). Experiments were conducted on 3 groups of young cattle. Each group has 10 heads selected by the method of analogues. When analyzing the data on the digestibility and absorption of nutrients, it was found that the animals of the experimental groups digested nutrients better compared to the animals of the control group: dry matter by 1.44% and 0.1%, organic matter by 2.0% and 1.65%, crude protein by 1.68% and 1.16%, crude fat by 2.28% and 1.33%, crude fiber by 2.48% and 2.04%, and nitrogen-free extractives by 1.88% and 1.61%. At the same time, the animals of the experimental groups used nitrogen better compared to the control group by 2,28% and 1,05%. The study of the biochemical composition of animal blood showed that complex feed additives are harmless. Thus, the inclusion of complex feed additives in the diets of young cattle can improve the indicators of digestibility and the degree of use of nutrients in the conditions of Yakutia.
众所周知,在雅库特的条件下,具有高生产力遗传潜力的牛无法完全实现这一点。这是由许多因素促成的,特别是有必要强调基本饲料,因为这些饲料缺乏营养和矿物质。因此,开发技术和方法,通过在牛的饮食中加入来自自然资源的复杂饲料添加剂来提高牛的生产力,对于风险农业的条件具有科学和实际的重要性。在这方面,目的是研究来自当地天然原料的复杂饲料添加剂对幼牛消化率和代谢的影响。这些研究使用了动物技术实践中普遍接受的方法,根据该方法进行了实验设置(M.F.Tomme,1969)。实验在3组幼牛身上进行。每组有10个通过类似物方法选择的头。在分析营养物质的消化率和吸收率数据时,发现实验组动物比对照组动物更好地消化营养物质:干物质消化率为1.44%和0.1%,有机物消化率为2.0%和1.65%,粗蛋白质消化率为1.68%和1.16%,粗脂肪消化率为2.28%和1.33%,粗纤维消化率为2.48%和2.04%,无氮提取物分别提高了1.88%和1.61%。同时,实验组动物对氮的利用率比对照组提高了2,28%和1,05%。对动物血液生化成分的研究表明,复合饲料添加剂是无害的。因此,在幼牛的日粮中加入复杂的饲料添加剂可以提高雅库特条件下的消化率指标和营养物质的利用程度。
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引用次数: 0
PREVALENCE OF ALEXITHYMIA AMONG ADOLESCENTS IN THE CENTRAL AND SOUTHERN REGIONS OF SIBERIA 西伯利亚中部和南部地区青少年述情障碍的患病率
Q4 Agricultural and Biological Sciences Pub Date : 2022-12-25 DOI: 10.12731/2658-6649-2022-14-6-191-210
Zhanna G. Zaitseva, Tatyana A. Kolodyazhnaya, O. Zaitseva, I. Ignatova
Aim. To study the prevalence of alexithymia among adolescents in the central (Krasnoyarsk city/Caucasians) and southern (Abakan, the Republic of Khakassia, Caucasians/Khakasses) regions of Siberia, taking into account ethnicity and gender differences. Materials and methods. Interviewing schoolchildren of various ethnic backgrounds aged 15-18 years is carried out using the Toronto Alexithymic Scale (TAS-26). Results. Mong Caucasian adolescents from different regions of residence (Krasnoyarsk and Abakan, the Republic of Khakassia), the prevalence rates of alexithymia were comparable (30,0% and 34,2%). For Khakas teenagers in Abakan, the Republic of Khakassia, this indicator (56.3%) was statistically significantly higher (p<0,001) in comparison with Caucasian teenagers, which we regard as a manifestation of the ethno-specific component of the Khakas personality in adolescence. Gender differences were established, manifested by a small number of non-alexithymic Khakass girls (6,8%) in comparison with Khakass boys (p=0,0360). Conclusion. Identification among practically healthy adolescents of subjects with signs of alexithymia is necessary for the timely implementation of preventive measures, and corrective measures should be targeted at girls and boys. Rational implementation of preventive measures and dynamic monitoring of alexithymic adolescents will reduce the risk of psychosomatic disorders and the frequency of adverse outcomes.
目标考虑到种族和性别差异,研究西伯利亚中部(克拉斯诺亚尔斯克市/高加索地区)和南部(阿巴坎、哈卡斯共和国、高加索地区/哈卡斯地区)青少年述情障碍的患病率。材料和方法。采用多伦多述情障碍量表(TAS-26)对15-18岁的不同种族背景的学童进行访谈。后果来自不同居住地(克拉斯诺亚尔斯克和阿巴坎,哈卡斯共和国)的蒙古族高加索青少年,述情障碍的患病率相当(30.0%和34.2%)。对于哈卡斯共和国阿巴坎的哈卡斯青少年来说,与高加索青少年相比,这一指标(56.3%)在统计学上显著更高(p<0001),我们认为这是青少年哈卡斯个性的种族特异性组成部分的表现。性别差异已经确定,与哈卡斯男孩相比,少数非述情障碍的哈卡斯女孩(6.8%)(p=0.0360)。结论在实际健康的青少年中识别有述情障碍迹象的受试者对于及时实施预防措施是必要的,纠正措施应针对女孩和男孩。合理实施预防措施并对述情障碍青少年进行动态监测,将降低身心障碍的风险和不良后果的发生频率。
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引用次数: 0
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Siberian Journal of Life Sciences and Agriculture
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