Pub Date : 2023-03-14DOI: 10.38150/sajeb.13(1).p39-45
K. Zerrouki, Ali Riazi
There is a growing problem of antibiotic resistance that requires new approaches and an urgent research on novel therapeutic strategies and antibacterial drugs. Olive fruit is one of the most popular mediterranian food. Phenolic metabolites (polyphenols) exhibit a series of biological properties that promotes human health. Olive tree (Olea europaea) can confer significant benefits to the human health, which motivated us to undertake the present study on its main benefits molecules (polyphenols) especially of other olive parts that are less used in humain therapy, since there are numerous findings about olive oil. The main objectiv of the present study was to valorize some parts of olive to evaluate their antibacterial effect in order to contribute to the development of an understanding of nutritional and clinical use. For this, total polyphenols were extracted from two algerian olive varieties (O. europaea); Chemlal and Sigoise using methanol. The polyphenol content was 0.17 ± 0.0006 and 0.05 ± 0.0031 μg / ml for the pulp and 0.0015 ± 0.12 and 0.08 ± 0,001μg / ml for the core respectively for the two varieties. Olive leaves had 0.08 ± 0.001 mg / ml of polyphenols. Leaves ethyl acetate extracts have shown high phenolic content (0.139 ± 0.011 μg / ml). Higher amounts (0.10 ± 0.001and 0.11 ± 0.0032 μg / ml) were registred for pulp and core respectively of the variety Sigoise. Antibacterial tests showed significant results represented by inhibition areas of 22 mm ± 1.41 for Staphylococcus aureus and Salmonella enteritidis and only 8.5 mm ± 0.71 for Listeria monocytogenes. No effect was observed for Escherichia coli. According to the results of this study, olive parts, especially leaves can be considered as a good source of antimicrobial agents that can be provided with a very low price and then can be used in medicine. For that, it is very interesting to continue valorizing other varieties of olive as described in the present study or for other uses.
{"title":"Antibacterial effect of phenolic extract of olive tree on some pathogenic bacteria","authors":"K. Zerrouki, Ali Riazi","doi":"10.38150/sajeb.13(1).p39-45","DOIUrl":"https://doi.org/10.38150/sajeb.13(1).p39-45","url":null,"abstract":"There is a growing problem of antibiotic resistance that requires new approaches and an urgent research on novel therapeutic strategies and antibacterial drugs. Olive fruit is one of the most popular mediterranian food. Phenolic metabolites (polyphenols) exhibit a series of biological properties that promotes human health. Olive tree (Olea europaea) can confer significant benefits to the human health, which motivated us to undertake the present study on its main benefits molecules (polyphenols) especially of other olive parts that are less used in humain therapy, since there are numerous findings about olive oil. The main objectiv of the present study was to valorize some parts of olive to evaluate their antibacterial effect in order to contribute to the development of an understanding of nutritional and clinical use. For this, total polyphenols were extracted from two algerian olive varieties (O. europaea); Chemlal and Sigoise using methanol. The polyphenol content was 0.17 ± 0.0006 and 0.05 ± 0.0031 μg / ml for the pulp and 0.0015 ± 0.12 and 0.08 ± 0,001μg / ml for the core respectively for the two varieties. Olive leaves had 0.08 ± 0.001 mg / ml of polyphenols. Leaves ethyl acetate extracts have shown high phenolic content (0.139 ± 0.011 μg / ml). Higher amounts (0.10 ± 0.001and 0.11 ± 0.0032 μg / ml) were registred for pulp and core respectively of the variety Sigoise. Antibacterial tests showed significant results represented by inhibition areas of 22 mm ± 1.41 for Staphylococcus aureus and Salmonella enteritidis and only 8.5 mm ± 0.71 for Listeria monocytogenes. No effect was observed for Escherichia coli. According to the results of this study, olive parts, especially leaves can be considered as a good source of antimicrobial agents that can be provided with a very low price and then can be used in medicine. For that, it is very interesting to continue valorizing other varieties of olive as described in the present study or for other uses.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80526018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-14DOI: 10.38150/sajeb.13(1).p20-32
Pankaj Kushwaha, Mansi Shrivastava, Diksha Pandey, Kalpana Verma, L. Dwivedi
The Aloe vera commonly known as Gwarpatha or Ghrit Kumari is considered as a universal panacea. It is a gifted plant for the health, beauty, medicinal and skincare industry. However, considering the multiple uses of it in other sectors also, it needed to be explored further for other than known industrial and medicinal values. The present study highlights the additional industrial and medicinal properties of the plant. It includes preliminary phytochemicals screening, GC-MS analysis of a Methanolic Extract of Aloe vera (MEAV) leaves and finding out the compounds activities at the international compounds databases of NCBI like PubChem, Drug Bank etc. Out of 200 compounds detected through GC-MS, mainly 46 compounds falling in major peak areas were further explored at the compounds databases. Accordingly, some key phytocompounds such as α-D Glucose, Lactose, Maltotriose, Citronellyl Butyrate, Phytol, Myristic Acid, Capric Acid, Heptadecanoic Acid etc. depicted the important industrial activities like sweet modulator of food stuff, diluents/sweetener for dairy, alcohol, soft drinks, health, beauty, confectionary and pharmaceutical industry; moisturizing and fragrance agent in cream, shampoo, soap, perfumed products; emulsifier, texture enhancer; agent for the lubricants, greases, dyes, plastics, detergent and biodiesel etc. Other than them, several phytocompounds showing medicinal properties like anti-cancer, anti-inflammatory, antioxidant, anti-hypertensive, anti-fungal, anti-bacterial, immunity booster, therapeutic agents for Rheumatoid Arthritis, acute respiratory distress syndrome, Asthma, Cardiovascular Disease, Cancer, Osteoporosis, Insomnia, etc. were also identified in the plant. Consequently, Aloe vera with its great industrial values can be a good source of raw material for several other industries like food, beverage, cosmetic, detergent, soaps, biodiesel pharmaceutical and may be considered for the sustainable aloe-cultivation as alternative cash crop in drought prone area like Bundelkhand region.
{"title":"The phytochemical profiling of Aloe vera through GC-MS and compounds activity validation at NCBI for industrial value addition of the plant","authors":"Pankaj Kushwaha, Mansi Shrivastava, Diksha Pandey, Kalpana Verma, L. Dwivedi","doi":"10.38150/sajeb.13(1).p20-32","DOIUrl":"https://doi.org/10.38150/sajeb.13(1).p20-32","url":null,"abstract":"The Aloe vera commonly known as Gwarpatha or Ghrit Kumari is considered as a universal panacea. It is a gifted plant for the health, beauty, medicinal and skincare industry. However, considering the multiple uses of it in other sectors also, it needed to be explored further for other than known industrial and medicinal values. The present study highlights the additional industrial and medicinal properties of the plant. It includes preliminary phytochemicals screening, GC-MS analysis of a Methanolic Extract of Aloe vera (MEAV) leaves and finding out the compounds activities at the international compounds databases of NCBI like PubChem, Drug Bank etc. Out of 200 compounds detected through GC-MS, mainly 46 compounds falling in major peak areas were further explored at the compounds databases. Accordingly, some key phytocompounds such as α-D Glucose, Lactose, Maltotriose, Citronellyl Butyrate, Phytol, Myristic Acid, Capric Acid, Heptadecanoic Acid etc. depicted the important industrial activities like sweet modulator of food stuff, diluents/sweetener for dairy, alcohol, soft drinks, health, beauty, confectionary and pharmaceutical industry; moisturizing and fragrance agent in cream, shampoo, soap, perfumed products; emulsifier, texture enhancer; agent for the lubricants, greases, dyes, plastics, detergent and biodiesel etc. Other than them, several phytocompounds showing medicinal properties like anti-cancer, anti-inflammatory, antioxidant, anti-hypertensive, anti-fungal, anti-bacterial, immunity booster, therapeutic agents for Rheumatoid Arthritis, acute respiratory distress syndrome, Asthma, Cardiovascular Disease, Cancer, Osteoporosis, Insomnia, etc. were also identified in the plant. Consequently, Aloe vera with its great industrial values can be a good source of raw material for several other industries like food, beverage, cosmetic, detergent, soaps, biodiesel pharmaceutical and may be considered for the sustainable aloe-cultivation as alternative cash crop in drought prone area like Bundelkhand region.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89469996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-14DOI: 10.38150/sajeb.13(1).p12-19
Md. Abu Naser, Shawon Ahmmed, Shahanaj Parvin, D. Mondal, M. Islam, Y. Mahmud
Paradise threadfin a species from the Perciformes order and polynemidae family is one of the commercially important fishes in the south-west coastal region of Bangladesh. The study was conducted to know the morphometric and meristic characteristics of this fish species. A total 120 fish samples were collected from May 2021 to April 2022 ranging from 11.2-23.5 cm TL and 9.77-100.87 g BW. The fin formula was: D1: 7; D2: 14-16; P1: 15-16/VII; P2: 6-9; A: 14-15; C: 16-18. Off the LWRs equations, BW = 0.0028×FL3.55 was the well-expressed model depending on the largeness of the R2 value. The value of slope (b) ranging from 3.07 to 3.55 indicates a positive allometric growth pattern. The LLRs were highly correlated (R2≥0.917) and regression of TL vs FL, SL, and Pr2ndDL showed the best-fitted model among the 8 equations. The correlations among 45 different LLRs, 37 correlations showed a very high positive correlation (r = 0.91-0.99) whereas, eight LLRs exhibited a high positive correlation (r = 0.84-0.90). This study will support future studies for the management of P. paradiseus in the coastal regions and the mangrove adjacent rivers.
{"title":"Morphometric and Meristic Characteristics of Paradise Threadfin (Polynemus paradiseus, Linnaeus 1758) in Sundarbans Estuary of Bangladesh","authors":"Md. Abu Naser, Shawon Ahmmed, Shahanaj Parvin, D. Mondal, M. Islam, Y. Mahmud","doi":"10.38150/sajeb.13(1).p12-19","DOIUrl":"https://doi.org/10.38150/sajeb.13(1).p12-19","url":null,"abstract":"Paradise threadfin a species from the Perciformes order and polynemidae family is one of the commercially important fishes in the south-west coastal region of Bangladesh. The study was conducted to know the morphometric and meristic characteristics of this fish species. A total 120 fish samples were collected from May 2021 to April 2022 ranging from 11.2-23.5 cm TL and 9.77-100.87 g BW. The fin formula was: D1: 7; D2: 14-16; P1: 15-16/VII; P2: 6-9; A: 14-15; C: 16-18. Off the LWRs equations, BW = 0.0028×FL3.55 was the well-expressed model depending on the largeness of the R2 value. The value of slope (b) ranging from 3.07 to 3.55 indicates a positive allometric growth pattern. The LLRs were highly correlated (R2≥0.917) and regression of TL vs FL, SL, and Pr2ndDL showed the best-fitted model among the 8 equations. The correlations among 45 different LLRs, 37 correlations showed a very high positive correlation (r = 0.91-0.99) whereas, eight LLRs exhibited a high positive correlation (r = 0.84-0.90). This study will support future studies for the management of P. paradiseus in the coastal regions and the mangrove adjacent rivers.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88619520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-16DOI: 10.38150/sajeb.12(6).p937-949
R. N. Benamara, M. Khader, Kouider Hadjadj, K. Righi, Bachir Benaraba
Southwest Algeria has a significant but little studied biodiversity, closely associated with a diverse cultural heritage. Medicinal plants are commonly used by the local population as traditional therapeutic treatments. This study was carried out with the aim of inventorying the spontaneous species used by the populations of the Ain Sefra region (south-western Algeria) and to gather information on the ancestral know-how of the local populations in terms of therapeutic use. The ethno-botanical survey was carried out using a semi-structured questionnaire among local herbalists practising in the study area. The data collected was analysed using quantitative indices such as species use value (UV), informant consensus factor (FIC) and fidelity level (FL). The results showed that 48 spontaneous medicinal plants were recognised and identified as medicinal species. These plants belonged to 28 families, of which the families Asteraceae and Lamiaceae are the most represented with 18% and 12% respectively. The leaves are the most used parts for medicinal purposes with decoction being the most used mode. Thus according to the (UV) Juniperus thurifera L. and Cotula cinerea Delile are the most used. Thus Artemisia herb alba Asso had the highest level of fidelity (100%) for the treatment of gastrointestinal diseases. In conclusion, this study revealed that the local population of the Ain Sefra region has a strong ethnobotanical knowledge and still uses local spontaneous medicinal species to treat several diseases.
{"title":"Spontaneous medicinal plants used by the population of Ain Sefra province (Southwest Algeria): An ethnobotanical study","authors":"R. N. Benamara, M. Khader, Kouider Hadjadj, K. Righi, Bachir Benaraba","doi":"10.38150/sajeb.12(6).p937-949","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p937-949","url":null,"abstract":"Southwest Algeria has a significant but little studied biodiversity, closely associated with a diverse cultural heritage. Medicinal plants are commonly used by the local population as traditional therapeutic treatments. This study was carried out with the aim of inventorying the spontaneous species used by the populations of the Ain Sefra region (south-western Algeria) and to gather information on the ancestral know-how of the local populations in terms of therapeutic use. The ethno-botanical survey was carried out using a semi-structured questionnaire among local herbalists practising in the study area. The data collected was analysed using quantitative indices such as species use value (UV), informant consensus factor (FIC) and fidelity level (FL). The results showed that 48 spontaneous medicinal plants were recognised and identified as medicinal species. These plants belonged to 28 families, of which the families Asteraceae and Lamiaceae are the most represented with 18% and 12% respectively. The leaves are the most used parts for medicinal purposes with decoction being the most used mode. Thus according to the (UV) Juniperus thurifera L. and Cotula cinerea Delile are the most used. Thus Artemisia herb alba Asso had the highest level of fidelity (100%) for the treatment of gastrointestinal diseases. In conclusion, this study revealed that the local population of the Ain Sefra region has a strong ethnobotanical knowledge and still uses local spontaneous medicinal species to treat several diseases.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91116638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-16DOI: 10.38150/sajeb.12(6).p920-927
Mayouré Edith, M. Yamkoulga, Elisabeth Zongo, A. Waongo, Z. Ilboudo, F. Traoré, A. Sanon
Senegalia macrostachya is a wild legume whose seeds are highly prized by the Burkinabe population. Unfortunately, they are severely depreciated during storage by the main pest Caryedon furcatus. However, very few studies have looked at the impact of the amount of S. macrostachya seeds on major biological parameters such as egg laying and development of C. furcatus. These data could contribute to better control of this pest. The aim of the study was to evaluate the impact of the quantity of S. macrostachya seeds on the egg laying behaviour of C. furcatus and on the ability of the larvae to weave their cocoons. Pairs of C. furcatus up to 24 hours old were placed separately with Petri dishes containing 5, 10, 20, 50, 100 and 200 seeds of S. macrostachya in four replicates per treatment. The seeds were renewed daily until the females died. After egg laying, the seeds were incubated until adult emergence. Our results showed that the rate of egg-bearing seeds increased significantly from 77.60% when the female had 5 seeds to 100% when she had 100 seeds. Similarly, the rate of larvae that were able to weave their cocoons increased significantly with the number of seeds. The variation in the amount of seeds and the number of pairs of C. furcatus has a strong impact on the behaviour and development of C. furcatus. The results suggest the need to control C. furcatus on S. macrostachya especially in case of mass storage for the benefit of the consumers.
{"title":"Influence of Senegalia macrostachya seed availability on Caryedon furcatus (Anton & Delobel) oviposition behaviour and its larvae survival","authors":"Mayouré Edith, M. Yamkoulga, Elisabeth Zongo, A. Waongo, Z. Ilboudo, F. Traoré, A. Sanon","doi":"10.38150/sajeb.12(6).p920-927","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p920-927","url":null,"abstract":"Senegalia macrostachya is a wild legume whose seeds are highly prized by the Burkinabe population. Unfortunately, they are severely depreciated during storage by the main pest Caryedon furcatus. However, very few studies have looked at the impact of the amount of S. macrostachya seeds on major biological parameters such as egg laying and development of C. furcatus. These data could contribute to better control of this pest. The aim of the study was to evaluate the impact of the quantity of S. macrostachya seeds on the egg laying behaviour of C. furcatus and on the ability of the larvae to weave their cocoons. Pairs of C. furcatus up to 24 hours old were placed separately with Petri dishes containing 5, 10, 20, 50, 100 and 200 seeds of S. macrostachya in four replicates per treatment. The seeds were renewed daily until the females died. After egg laying, the seeds were incubated until adult emergence. Our results showed that the rate of egg-bearing seeds increased significantly from 77.60% when the female had 5 seeds to 100% when she had 100 seeds. Similarly, the rate of larvae that were able to weave their cocoons increased significantly with the number of seeds. The variation in the amount of seeds and the number of pairs of C. furcatus has a strong impact on the behaviour and development of C. furcatus. The results suggest the need to control C. furcatus on S. macrostachya especially in case of mass storage for the benefit of the consumers.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87168584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibiotic resistance by pathogenic microorganisms and the toxic properties of several synthetic antioxidants have become a serious problem. The plant species constitute an inexhaustible source of molecules with very varied biological and pharmacological activities. This study aims to estimate the effect of substrate polarity on antioxidant and antimicrobial activity of three medicinal plants: Artemisia campestris, Retamas phaerocarpa, Saccocalyx saturoides. As well as the total phenolic contents were evaluated. Antioxidant capacity was assessed by diphenyle-picryl-hydrazyl (DPPH). Antibacterial activity was evaluated by the agar diffusion method. The total phenol content were determined by the Folin–Ciocalteu method. The results revealed that scavenging capacity of DPPH free radical depends of nature of the solvents. The percent of inhibition ranges from 46.56 to 93.20%. The ethyl acetate fraction of A. campestris exhibits the highest antioxidant activity. A.campestris extract showed the highest phenol content (731.66 μg EAG / ml extracted). Whereas all extracts tested were inactive on Escherichia Coli, Staphyococus. Extracts of A.campestris have great potential as an antioxidant drug.
{"title":"Total phenolic content, antioxidant potential and antibacterial activity of three medicinal plants grown widely in Algeria steppe","authors":"Souhila Touil, Aissaoui Regadia, Rebhi Almahdaoui Abdelghani","doi":"10.38150/sajeb.12(6).p912-919","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p912-919","url":null,"abstract":"Antibiotic resistance by pathogenic microorganisms and the toxic properties of several synthetic antioxidants have become a serious problem. The plant species constitute an inexhaustible source of molecules with very varied biological and pharmacological activities. This study aims to estimate the effect of substrate polarity on antioxidant and antimicrobial activity of three medicinal plants: Artemisia campestris, Retamas phaerocarpa, Saccocalyx saturoides. As well as the total phenolic contents were evaluated. Antioxidant capacity was assessed by diphenyle-picryl-hydrazyl (DPPH). Antibacterial activity was evaluated by the agar diffusion method. The total phenol content were determined by the Folin–Ciocalteu method. The results revealed that scavenging capacity of DPPH free radical depends of nature of the solvents. The percent of inhibition ranges from 46.56 to 93.20%. The ethyl acetate fraction of A. campestris exhibits the highest antioxidant activity. A.campestris extract showed the highest phenol content (731.66 μg EAG / ml extracted). Whereas all extracts tested were inactive on Escherichia Coli, Staphyococus. Extracts of A.campestris have great potential as an antioxidant drug.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85412074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-16DOI: 10.38150/sajeb.12(6).p950-961
Bachir Benkaddour, Miloud Heddadji, M. Kihal
The genus Bifidobacterium and Lactobacillus are part of the microbiote of the gastrointestinal tract in human and animal. Certain members of these genera are beneficial to human health and are used as probiotic agents. Camel milk, in addition to its nutritional value, possesses incontestable therapeutic potential against several diseases. These important benefits prompted us to conduct this present research with the aim to firstly, charaterize tow indigenous strains and to screen their probiotic attributes and, secondly, evaluate their viability in camel milk during cold storage. Phenotypic and genotypic identification showed that strain Lb49 belongs to Lactbacillus paracasei. subsp. Paracasei, and the strain Bif21 belongs to Bifidobacterium asteroides. The study showed the safety aspect of the two identified strains, high resistance towards bile (SR> 71%), lysozyme (SR> 81%), low to moderate auto-aggregation (10.9-44.4%) and hydrophobicity (23.4-41.9) as well as antimicrobial activity against the tested pathogens with the highest scores recorded by the strain Lb49. When exposed to low pH conditions, only the strain Lb49 exhibited high tolerance with SR> 77%. Regarding the strain’s viability in camel milk, they maintained an excellent viability (93.5-100.5 %) after 15 days storage at 4° C. The characteristics demonstrated by Lb49 enable it to be used as a potential probiotic as well as Bif21 strain, as far as protection is provided for their survival by filling their intolerance to acidic stress using an adequate technique. Moreover, camel milk guaranteed excellent viability for the studied strains thanks to its physicochemical composition, acting as a good medium for nutrition and transport of probiotics.
{"title":"Characterization, probiotic assessment and viability of Bifidobacterium asteroides Bif21 and Lactobacillus paracasei. subsp. paracasei Lb49 strains, isolated from Algerian honeybee’s gut and infant faeces, in fermented camel milk","authors":"Bachir Benkaddour, Miloud Heddadji, M. Kihal","doi":"10.38150/sajeb.12(6).p950-961","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p950-961","url":null,"abstract":"The genus Bifidobacterium and Lactobacillus are part of the microbiote of the gastrointestinal tract in human and animal. Certain members of these genera are beneficial to human health and are used as probiotic agents. Camel milk, in addition to its nutritional value, possesses incontestable therapeutic potential against several diseases. These important benefits prompted us to conduct this present research with the aim to firstly, charaterize tow indigenous strains and to screen their probiotic attributes and, secondly, evaluate their viability in camel milk during cold storage. Phenotypic and genotypic identification showed that strain Lb49 belongs to Lactbacillus paracasei. subsp. Paracasei, and the strain Bif21 belongs to Bifidobacterium asteroides. The study showed the safety aspect of the two identified strains, high resistance towards bile (SR> 71%), lysozyme (SR> 81%), low to moderate auto-aggregation (10.9-44.4%) and hydrophobicity (23.4-41.9) as well as antimicrobial activity against the tested pathogens with the highest scores recorded by the strain Lb49. When exposed to low pH conditions, only the strain Lb49 exhibited high tolerance with SR> 77%. Regarding the strain’s viability in camel milk, they maintained an excellent viability (93.5-100.5 %) after 15 days storage at 4° C. The characteristics demonstrated by Lb49 enable it to be used as a potential probiotic as well as Bif21 strain, as far as protection is provided for their survival by filling their intolerance to acidic stress using an adequate technique. Moreover, camel milk guaranteed excellent viability for the studied strains thanks to its physicochemical composition, acting as a good medium for nutrition and transport of probiotics.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"83 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83550875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tuberose (Polianthes tuberosa L.) is an ornamental and medicinal plant, cultivated in tropical and subtropical regions for its fragrant cut flowers and perfume industries, P. tuberosa flowers are the source of the highest-quality tuberose oil that remains today the most expensive raw material in perfumery. Furthermore, bulb size influences the flowering of P. tuberosa L. and creates a problem for tuberose flower production. Aims in this study were climatic condition and bulbs size variation, which were investigated to enhance the bulb size and climatic condition for improving tuberose production in Algeria. A culture of P. tuberosa L. bulbs was conducted in the field and under shelter using different bulb diameters, growing and flowering parameters were recorded for each plant, from planting to maturity. According to the obtained results, a good growth of tuberose was noticed with the big bulbs (diameter≥2 cm), i.e., bulb germination, leaf emergence, and the number of leaves by dominance for the bulbs cultivated under shelter, compared to those grown in the field. Regarding flowering, it was influenced mainly by bulb size, where only big bulbs gave flowers, and flowering characteristics were improved by the under shelter culture neither in the field. In general and according to the present work, for a good growth and for a better production of P. tuberosa flowers, bulbs culture under shelter using bulbs of 2-4 cm in diameter were recommended.
{"title":"Effect of Bulb Size and Climatic Conditions on Vegetative and Reproductive Attributes of Polianthes tuberosa L. cultivated in Algeria","authors":"N. Barghout, MESSGO-MOUMENE Saida, GHARBI Abdelaziz, ALLAL-BENFKIH Laila, KHENNOUF Seddik, EL-HADI Djamel","doi":"10.38150/sajeb.12(6).p932-936","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p932-936","url":null,"abstract":"Tuberose (Polianthes tuberosa L.) is an ornamental and medicinal plant, cultivated in tropical and subtropical regions for its fragrant cut flowers and perfume industries, P. tuberosa flowers are the source of the highest-quality tuberose oil that remains today the most expensive raw material in perfumery. Furthermore, bulb size influences the flowering of P. tuberosa L. and creates a problem for tuberose flower production. Aims in this study were climatic condition and bulbs size variation, which were investigated to enhance the bulb size and climatic condition for improving tuberose production in Algeria. A culture of P. tuberosa L. bulbs was conducted in the field and under shelter using different bulb diameters, growing and flowering parameters were recorded for each plant, from planting to maturity. According to the obtained results, a good growth of tuberose was noticed with the big bulbs (diameter≥2 cm), i.e., bulb germination, leaf emergence, and the number of leaves by dominance for the bulbs cultivated under shelter, compared to those grown in the field. Regarding flowering, it was influenced mainly by bulb size, where only big bulbs gave flowers, and flowering characteristics were improved by the under shelter culture neither in the field. In general and according to the present work, for a good growth and for a better production of P. tuberosa flowers, bulbs culture under shelter using bulbs of 2-4 cm in diameter were recommended.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"146 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78288847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-16DOI: 10.38150/sajeb.12(6).p896-911
Sarada Jonnalagadda, A. S.
Development of therapeutic agents against a model organism like P.aeruginosa, which forms biofilms, has been reviewed in the current study. A scientometric analysis has been used to predict the developments in Pseudomonas biofilm research, to configure potential antibiofilm agents (ABA) that can have prominent inhibition or dispersal effect and also overcome the problem of drug resistance. Diversity of ABA, antibiofilm strategy, prominent investigators and institutions in Pseudomonas biofilm research during the period 2009-2019 are the areas of focus, to understand the contributions in antibiofilm therapy. Scientometric study and the meta-analysis from 25,113 research publications, indicate nanoparticles as most promising control in biofilm based hazard. The results of investigation among majority of research groups reveal prominence of combinatorial strategy in targeting biofilms. A major shift from conventional antibiotics to nanoparticles as potential ABA is one of the significant findings of the study. Co-word analysis and Sankey plots were instrumental to identify the network of strategies focusing on biofilm inhibition. A Pearson correlation analysis for percentage of biofilm inhibition shows significant relevance with the nanoparticle type but not size. Moreover the impact of antibiofilm activity could be significantly correlated with source, nature and method of synthesis.
{"title":"A Scientometric analysis on Antibiofilm Agents developed against Pseudomonas aeruginosa","authors":"Sarada Jonnalagadda, A. S.","doi":"10.38150/sajeb.12(6).p896-911","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p896-911","url":null,"abstract":"Development of therapeutic agents against a model organism like P.aeruginosa, which forms biofilms, has been reviewed in the current study. A scientometric analysis has been used to predict the developments in Pseudomonas biofilm research, to configure potential antibiofilm agents (ABA) that can have prominent inhibition or dispersal effect and also overcome the problem of drug resistance. Diversity of ABA, antibiofilm strategy, prominent investigators and institutions in Pseudomonas biofilm research during the period 2009-2019 are the areas of focus, to understand the contributions in antibiofilm therapy. Scientometric study and the meta-analysis from 25,113 research publications, indicate nanoparticles as most promising control in biofilm based hazard. The results of investigation among majority of research groups reveal prominence of combinatorial strategy in targeting biofilms. A major shift from conventional antibiotics to nanoparticles as potential ABA is one of the significant findings of the study. Co-word analysis and Sankey plots were instrumental to identify the network of strategies focusing on biofilm inhibition. A Pearson correlation analysis for percentage of biofilm inhibition shows significant relevance with the nanoparticle type but not size. Moreover the impact of antibiofilm activity could be significantly correlated with source, nature and method of synthesis.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76689872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-16DOI: 10.38150/sajeb.12(6).p928-931
Khadidja Sedrati, Ouarda Semmame, H. Ziada, Ibtissem Boudokhane, N. Abadi
TP53 is a tumor suppressor gene involved in the development of several types of cancer. It has been reported that single nucleotide polymorphism in codon 72 of this gene, causing substitution of a Proline for an Arginine, may increase the risk of carcinogenesis. The objective of this study was to assess the frequency of the Arg72Pro polymorphism and to look for a possible association between this polymorphism and the occurrence of cancer. Our study concerned 82 DNA samples collected from the DNA bank of the Laboratory of Biology and Molecular Genetics of the Constantine University 3, and grouped 2 populations: 41 controls and 41 patients with one of three types of cancer: breast cancer, cervical cancer and lung cancer. This polymorphism was detected with PCR / RFLP. The results obtained in our study revealed a significant difference between the two patient and control groups for the heterozygous CG genotype (OR = 0.13, P = 0.001), which makes it possible to conclude that the CG genotype constitutes a risk factor in the occurrence cancer. However, the study showed that there is no difference between the Pro allele and the Arg allele in the patients compared to the controls (OR = 0.95, P = 0.87), which could rule out the possible association between the Arg72Pro polymorphism and the onset of cancer.
TP53是一种肿瘤抑制基因，参与多种癌症的发展。据报道，该基因密码子72的单核苷酸多态性导致脯氨酸被精氨酸取代，可能增加致癌风险。本研究的目的是评估Arg72Pro多态性的频率，并寻找这种多态性与癌症发生之间的可能关联。我们的研究涉及从康斯坦丁大学生物学和分子遗传学实验室的DNA库中收集的82个DNA样本，并将2个人群分为两组:41个对照组和41个患有乳腺癌、宫颈癌和肺癌三种癌症之一的患者。采用PCR / RFLP检测该多态性。我们的研究结果显示，两组患者与对照组的杂合子CG基因型有显著差异(OR = 0.13, P = 0.001)，可以得出CG基因型是癌症发生的危险因素的结论。然而，本研究显示，与对照组相比，患者的Pro等位基因和Arg等位基因没有差异(OR = 0.95, P = 0.87)，这可以排除Arg72Pro多态性与癌症发病之间可能存在的关联。
{"title":"Impact of Arg72Pro polymorphism in P53 gene in cancer susceptibility in Algerian population","authors":"Khadidja Sedrati, Ouarda Semmame, H. Ziada, Ibtissem Boudokhane, N. Abadi","doi":"10.38150/sajeb.12(6).p928-931","DOIUrl":"https://doi.org/10.38150/sajeb.12(6).p928-931","url":null,"abstract":"TP53 is a tumor suppressor gene involved in the development of several types of cancer. It has been reported that single nucleotide polymorphism in codon 72 of this gene, causing substitution of a Proline for an Arginine, may increase the risk of carcinogenesis. The objective of this study was to assess the frequency of the Arg72Pro polymorphism and to look for a possible association between this polymorphism and the occurrence of cancer. Our study concerned 82 DNA samples collected from the DNA bank of the Laboratory of Biology and Molecular Genetics of the Constantine University 3, and grouped 2 populations: 41 controls and 41 patients with one of three types of cancer: breast cancer, cervical cancer and lung cancer. This polymorphism was detected with PCR / RFLP. The results obtained in our study revealed a significant difference between the two patient and control groups for the heterozygous CG genotype (OR = 0.13, P = 0.001), which makes it possible to conclude that the CG genotype constitutes a risk factor in the occurrence cancer. However, the study showed that there is no difference between the Pro allele and the Arg allele in the patients compared to the controls (OR = 0.95, P = 0.87), which could rule out the possible association between the Arg72Pro polymorphism and the onset of cancer.","PeriodicalId":21895,"journal":{"name":"South Asian Journal of Experimental Biology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86145565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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