Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2127
Marinha Ximenes de Lima Oliveira, Aires Santos Silva, Marcos Adriano Pereira Barbosa, T. C. Santos
This study investigated the effects of pre-heating fertile Japanese quail eggs during storage on embryonic development, incubation performance, hatched chick quality, and chick performance up to 35 days of age. The experiment was laid out in a completely randomized design in a 3 × 2 + 1 factorial arrangement, totaling seven treatments. These treatments included three storage periods (3.5, 6.5, and 9.5 days) and two pre-heating periods (0 and 4 h at 37.5°C and 60% RH). Pre-heating was conducted on the third day of egg storage inside the incubator machine, with the control treatment involving the storage of fertile eggs for 12 h without pre-heating. Extended egg storage for more than six days led to an increase in the percentages of yolk and shell, elevated albumen pH, reduced albumen percentage, diminished hatchability rate, and an increased embryonic mortality rate. Pre-heating the eggs resulted in a reduction in the weight, length, and amount of residual yolk sac of the newly hatched chick. No significant effects of pre-heating fertile Japanese quail eggs during storage were observed on the studied variables. Therefore, the protocol involving a 4-h pre-heating at 37.5 ºC and 60% relative humidity during the storage period does not yield improvements in incubation rates or chick quality in Japanese quail. Further studies are warranted to determine the optimal protocol for pre-heating Japanese quail eggs.
{"title":"Effects of short heating periods during egg storage on quail embryonic development, incubation performance, chick quality, and chick performance up to 35 days","authors":"Marinha Ximenes de Lima Oliveira, Aires Santos Silva, Marcos Adriano Pereira Barbosa, T. C. Santos","doi":"10.5433/1679-0359.2023v44n6p2127","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2127","url":null,"abstract":"This study investigated the effects of pre-heating fertile Japanese quail eggs during storage on embryonic development, incubation performance, hatched chick quality, and chick performance up to 35 days of age. The experiment was laid out in a completely randomized design in a 3 × 2 + 1 factorial arrangement, totaling seven treatments. These treatments included three storage periods (3.5, 6.5, and 9.5 days) and two pre-heating periods (0 and 4 h at 37.5°C and 60% RH). Pre-heating was conducted on the third day of egg storage inside the incubator machine, with the control treatment involving the storage of fertile eggs for 12 h without pre-heating. Extended egg storage for more than six days led to an increase in the percentages of yolk and shell, elevated albumen pH, reduced albumen percentage, diminished hatchability rate, and an increased embryonic mortality rate. Pre-heating the eggs resulted in a reduction in the weight, length, and amount of residual yolk sac of the newly hatched chick. No significant effects of pre-heating fertile Japanese quail eggs during storage were observed on the studied variables. Therefore, the protocol involving a 4-h pre-heating at 37.5 ºC and 60% relative humidity during the storage period does not yield improvements in incubation rates or chick quality in Japanese quail. Further studies are warranted to determine the optimal protocol for pre-heating Japanese quail eggs.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140483227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2179
Jeycy Kelle Sirqueira Mendonça, F. L. Nunes, Carolina Merlin Meurer, J. C. Ribeiro Júnior, A. Correia
O Brasil é um dos maiores produtores de carne do mundo. Tendo em vista essa grande produtividade e a preocupação com a qualidade da carne produzida, os estabelecimentos produtores estão buscando meios para maior conservação do produto, sendo a embalagem a vácuo um dos mais utilizados. O objetivo do trabalho foi acompanhar a microbiota indicadora e patogênica durante a shelf life de Longissimus dorsi bovino embalado a vácuo. Foram avaliadas amostras de contrafilé coletadas e acondicionadas na seção de desossa de um frigorífico sob inspeção federal. Cada amostra foi fracionada em quatro peças e cada peça foi utilizada para compor uma parte de cada um dos pools, sendo totalizado quatro pools mantidos à 7ºC e analisados de 0 até os 60 dias de embalagem primária, com intervalo de 20 dias. Foram quantificados aeróbios mesófilos, psicrotróficos, enterobactérias, coliformes a 30°C, Escherichia coli e Staphylococcus spp. Através de abordagens moleculares foram caracterizados os patótipos de E. coli produtora da toxina shiga (STEC), enteropatogênica (EPEC), enterohemorrágica (EHEC), enteroagregativa (EAEC), enterotoxigênica (ETEC) e enteroinvasiva (EIEC), Pseudomonas spp. entre os psicrotróficos, Salmonella spp. e Listeria monocytogenes. As quantificações dos micro-organismos indicadores foram aumentando progressivamente a cada intervalo de análise, com destaque para os psicrotróficos que aumentaram de 5 x 101 no dia 0 para 4,2 x 108 UFC/g no dia 60, predominando Pseudomonas spp. (48%). As contagens que possuem padrão determinado por legislações vigentes tiveram seus limites ultrapassados, como E. coli, desde o dia 20 (7 x 102 UFC/g). Foram identificadas EPEC, ETEC, STEC e EIEC, além de L. monocytogenes em todas as análises e Salmonella spp., essa última só não detectada no primeiro dia de shelf life. Fazem-se necessárias, portanto, revisões nos planos de autocontrole assim como maior rigor microbiológico na produção e processamento da carne bovina para melhoria da shelf life do produto e aumento da sua segurança.
{"title":"Acompanhamento da microbiota indicadora e patogênica durante a vida útil de Longissimus dorsi (contrafilé) embalado a vácuo","authors":"Jeycy Kelle Sirqueira Mendonça, F. L. Nunes, Carolina Merlin Meurer, J. C. Ribeiro Júnior, A. Correia","doi":"10.5433/1679-0359.2023v44n6p2179","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2179","url":null,"abstract":"O Brasil é um dos maiores produtores de carne do mundo. Tendo em vista essa grande produtividade e a preocupação com a qualidade da carne produzida, os estabelecimentos produtores estão buscando meios para maior conservação do produto, sendo a embalagem a vácuo um dos mais utilizados. O objetivo do trabalho foi acompanhar a microbiota indicadora e patogênica durante a shelf life de Longissimus dorsi bovino embalado a vácuo. Foram avaliadas amostras de contrafilé coletadas e acondicionadas na seção de desossa de um frigorífico sob inspeção federal. Cada amostra foi fracionada em quatro peças e cada peça foi utilizada para compor uma parte de cada um dos pools, sendo totalizado quatro pools mantidos à 7ºC e analisados de 0 até os 60 dias de embalagem primária, com intervalo de 20 dias. Foram quantificados aeróbios mesófilos, psicrotróficos, enterobactérias, coliformes a 30°C, Escherichia coli e Staphylococcus spp. Através de abordagens moleculares foram caracterizados os patótipos de E. coli produtora da toxina shiga (STEC), enteropatogênica (EPEC), enterohemorrágica (EHEC), enteroagregativa (EAEC), enterotoxigênica (ETEC) e enteroinvasiva (EIEC), Pseudomonas spp. entre os psicrotróficos, Salmonella spp. e Listeria monocytogenes. As quantificações dos micro-organismos indicadores foram aumentando progressivamente a cada intervalo de análise, com destaque para os psicrotróficos que aumentaram de 5 x 101 no dia 0 para 4,2 x 108 UFC/g no dia 60, predominando Pseudomonas spp. (48%). As contagens que possuem padrão determinado por legislações vigentes tiveram seus limites ultrapassados, como E. coli, desde o dia 20 (7 x 102 UFC/g). Foram identificadas EPEC, ETEC, STEC e EIEC, além de L. monocytogenes em todas as análises e Salmonella spp., essa última só não detectada no primeiro dia de shelf life. Fazem-se necessárias, portanto, revisões nos planos de autocontrole assim como maior rigor microbiológico na produção e processamento da carne bovina para melhoria da shelf life do produto e aumento da sua segurança.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140485388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2217
L. L. D. M. Frasca, Cássia Cristina Rezende, Mariana Aguiar Silva, A. C. Lanna, Dennis Ricardo Cabral Cruz, A. S. Nascente
Beneficial rhizobacteria are multifunctional microorganisms that stimulate plant growth through direct mechanisms such as the solubilization of phosphorus and potassium present in soil minerals, biological nitrogen fixation and production of plant hormones. The objective of this study was to determine the effect of beneficial rhizobacteria on the initial development, promoting growth and resistance of soybean seedlings, aiming not only for increased production but also for agricultural sustainability through the maximization of biological efficiency in the early development of soybean seedlings. The experiment, under controlled conditions, was conducted in a completely randomized design, with four treatments and ten replications. The treatments consisted in the microbiolization of soybean seeds with: 1. Control (without microorganism); 2. Serratia marcenses (BRM 32114); 3. Bacillus spp. (BRM63573) and 4. BRM 32114 + (BRM63573). After the microbiolization of the seeds, they were sown and placed to germinate, after 12 days, the seedlings were harvested and divided into shoots and roots. The roots were washed and photographed with digital camera. The images were analyzed and determined length, diameter, surface area and volume of roots. Then, root and shoot were dried and weighed for biomass determination. Co-inculation, BRM 32114+ BRM63573, promoted greater robustness of the root system of soybean seedlings with increased length (16.2%), diameter (22.5%), volume (43.4%) and dry biomass (29.7%). The dry matter of shoots and total soybean seedlings was superior to the control treatment in all treatments with rhizobacteria. Therefore, plant growth promoting
{"title":"Interação de plântulas de soja com rizobactérias benéficas","authors":"L. L. D. M. Frasca, Cássia Cristina Rezende, Mariana Aguiar Silva, A. C. Lanna, Dennis Ricardo Cabral Cruz, A. S. Nascente","doi":"10.5433/1679-0359.2023v44n6p2217","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2217","url":null,"abstract":"Beneficial rhizobacteria are multifunctional microorganisms that stimulate plant growth through direct mechanisms such as the solubilization of phosphorus and potassium present in soil minerals, biological nitrogen fixation and production of plant hormones. The objective of this study was to determine the effect of beneficial rhizobacteria on the initial development, promoting growth and resistance of soybean seedlings, aiming not only for increased production but also for agricultural sustainability through the maximization of biological efficiency in the early development of soybean seedlings. The experiment, under controlled conditions, was conducted in a completely randomized design, with four treatments and ten replications. The treatments consisted in the microbiolization of soybean seeds with: 1. Control (without microorganism); 2. Serratia marcenses (BRM 32114); 3. Bacillus spp. (BRM63573) and 4. BRM 32114 + (BRM63573). After the microbiolization of the seeds, they were sown and placed to germinate, after 12 days, the seedlings were harvested and divided into shoots and roots. The roots were washed and photographed with digital camera. The images were analyzed and determined length, diameter, surface area and volume of roots. Then, root and shoot were dried and weighed for biomass determination. Co-inculation, BRM 32114+ BRM63573, promoted greater robustness of the root system of soybean seedlings with increased length (16.2%), diameter (22.5%), volume (43.4%) and dry biomass (29.7%). The dry matter of shoots and total soybean seedlings was superior to the control treatment in all treatments with rhizobacteria. Therefore, plant growth promoting","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140484238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2207
Eliana Burtet Parmeggiani, Cláudia Medeiros Rodrigues, Ana Martiele Engelmann, Leonardo Sasso Bernardi, J. M. Trentin, Francielli Weber Santos Cibin, Alan Miranda Prestes, João Batista Teixeira da Rocha, Cinthia Melazzo de Andrade, M. Leal
O objetivo do trabalho foi avaliar o efeito do disseleneto de difenila (PhSe)2, no ganho médio diário, parâmetros bioquímicos e status oxidativo de bezerras de corte submetidas ao desmame convencional. Foram utilizadas 30 bezerras, com seis meses de vida e 152,42±13,75 kg de peso corporal. Foi utilizado um delineamento inteiramente casualizado sendo as unidades experimentais distribuídas em três grupos: grupo controle (GC, n=10): 2 mL de solução de NaCl; grupo dimetilsulfóxido (GDMSO, n=10): 2 mL de dimetilsulfóxido; e grupo disseleneto de difenila (GDD, n=10): 3 µmol kg-1 de (PhSe)2 diluído em 2 mL de dimetilsulfóxido. As coletas e avaliações foram realizadas no M1=basal (-28) e M2 (-14) dia antes do desmame, M3 (0) dia inicial do desmame, M4 (14) e M5 (28) dia após o desmame. Os tratamentos foram administrados no M1, M2 e M3 pela via subcutânea. Todos os animais foram submetidos a condições similares de manejo e alimentação ao decorrer do experimento, tendo livre acesso a água. Os parâmetros avaliados foram: ganho médio diário, proteína total, albumina, globulina, relação albumina:globulina, glutationa reduzida, substâncias reativas ao ácido tiobarbitúrio e capacidade antioxidante total, determinada pelo potencial antioxidante redutor férrico. Dentre os parâmetros avaliados observou-se efeito significativo (P<0,05): no ganho médio diário entre momentos; na albumina entre tratamentos, momentos e interação tratamento x momento; nas globulinas entre momentos e interação tratamento x momento; na relação albumina:globulina entre momentos e interação tratamento x momento; na glutationa reduzida entre momentos e interação tratamento x momento e no potencial antioxidante redutor férrico entre momentos. A administração de disseleneto de difenila em bezerras de corte proporcionou incremento nas variáveis albumina, globulina, relação albumina:globulina e glutationa reduzida durante o desmame convencional, sendo o efeito benéfico do disseleneto de difenila constatado a partir das interações entre tratamento x momentos. Esses resultados demostram que o disseleneto de difenila é uma alternativa as fontes tradicionais de selênio orgânico, e quando utilizado reduziu os desafios vivenciados pelas bezerras de corte durante o desmame convencional.
本研究旨在评估二苯基二硒化物（PhSe）2 对常规断奶的肉用小母牛的平均日增重、生化指标和氧化状态的影响。使用了 30 头六个月大的小母牛，体重为 152.42±13.75 千克。采用完全随机设计，实验单位分为三组：对照组（CG，n=10）：2 mL NaCl 溶液；二甲基亚砜组（GDMSO，n=10）：2 mL 二甲基亚砜；二苯基二硒组（GDD，n=10）：3 µmol kg-1 (PhSe)2 稀释在 2 mL 二甲基亚砜中。在断奶前的 M1=基本（-28）天和 M2（-14）天、断奶后的 M3（0）天、断奶后的 M4（14）天和 M5（28）天进行收集和评估。治疗分别在 M1、M2 和 M3 进行皮下注射。在整个实验过程中，所有动物的操作和饲喂条件相似，并可自由饮水。评估参数包括：平均日增重、总蛋白、白蛋白、球蛋白、白蛋白与球蛋白比率、还原型谷胱甘肽、硫代巴比妥酸活性物质和总抗氧化能力（通过铁还原抗氧化潜能测定）。在评估的参数中，下列参数有显著影响（P<0.05）：不同时间间的平均日增重；不同处理、时间和处理×时间交互作用间的白蛋白；不同时间和处理×时间交互作用间的球蛋白；不同时间和处理×时间交互作用间的白蛋白：球蛋白比；不同时间和处理×时间交互作用间的还原型谷胱甘肽；以及不同时间间的铁还原抗氧化潜能。对肉用小母牛施用二苯基二硒醚可导致常规断奶期白蛋白、球蛋白、白蛋白：球蛋白比率和还原型谷胱甘肽等变量的增加，二苯基二硒醚的有益作用体现在处理 x 时间的交互作用中。这些结果表明，二苯基二硒化物是有机硒传统来源的替代品，使用二苯基二硒化物可减少肉用小母牛在常规断奶期间遇到的挑战。
{"title":"Efeito metafilático do disseleneto de difenila (PhSe)2 na saúde de bezerras de corte submetidas ao desmame convencional","authors":"Eliana Burtet Parmeggiani, Cláudia Medeiros Rodrigues, Ana Martiele Engelmann, Leonardo Sasso Bernardi, J. M. Trentin, Francielli Weber Santos Cibin, Alan Miranda Prestes, João Batista Teixeira da Rocha, Cinthia Melazzo de Andrade, M. Leal","doi":"10.5433/1679-0359.2023v44n6p2207","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2207","url":null,"abstract":"O objetivo do trabalho foi avaliar o efeito do disseleneto de difenila (PhSe)2, no ganho médio diário, parâmetros bioquímicos e status oxidativo de bezerras de corte submetidas ao desmame convencional. Foram utilizadas 30 bezerras, com seis meses de vida e 152,42±13,75 kg de peso corporal. Foi utilizado um delineamento inteiramente casualizado sendo as unidades experimentais distribuídas em três grupos: grupo controle (GC, n=10): 2 mL de solução de NaCl; grupo dimetilsulfóxido (GDMSO, n=10): 2 mL de dimetilsulfóxido; e grupo disseleneto de difenila (GDD, n=10): 3 µmol kg-1 de (PhSe)2 diluído em 2 mL de dimetilsulfóxido. As coletas e avaliações foram realizadas no M1=basal (-28) e M2 (-14) dia antes do desmame, M3 (0) dia inicial do desmame, M4 (14) e M5 (28) dia após o desmame. Os tratamentos foram administrados no M1, M2 e M3 pela via subcutânea. Todos os animais foram submetidos a condições similares de manejo e alimentação ao decorrer do experimento, tendo livre acesso a água. Os parâmetros avaliados foram: ganho médio diário, proteína total, albumina, globulina, relação albumina:globulina, glutationa reduzida, substâncias reativas ao ácido tiobarbitúrio e capacidade antioxidante total, determinada pelo potencial antioxidante redutor férrico. Dentre os parâmetros avaliados observou-se efeito significativo (P<0,05): no ganho médio diário entre momentos; na albumina entre tratamentos, momentos e interação tratamento x momento; nas globulinas entre momentos e interação tratamento x momento; na relação albumina:globulina entre momentos e interação tratamento x momento; na glutationa reduzida entre momentos e interação tratamento x momento e no potencial antioxidante redutor férrico entre momentos. A administração de disseleneto de difenila em bezerras de corte proporcionou incremento nas variáveis albumina, globulina, relação albumina:globulina e glutationa reduzida durante o desmame convencional, sendo o efeito benéfico do disseleneto de difenila constatado a partir das interações entre tratamento x momentos. Esses resultados demostram que o disseleneto de difenila é uma alternativa as fontes tradicionais de selênio orgânico, e quando utilizado reduziu os desafios vivenciados pelas bezerras de corte durante o desmame convencional.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140484847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2249
Vanessa Casiraghi Zanon, J. M. Kafer, Jaqueline Hagn, Jéssica Cardoso, R. Dallemole-Giaretta, Taciane Finatto, T. Vargas
Bacterial wilt, caused by phytopathogenic species of the genus Ralstonia, is one of the main diseases affecting the tomato crop. The Ralstonia solanacearum species complex occurs as a result of variants being widely diverse in terms of adaptation to different climatic conditions, host variations, and aggressiveness, which complicate disease control recommendations. In this study, we employed molecular methods to analyze 26 R. solanacearum isolates collected from tomato plants cultivated in the southwest region of Paraná, Brazil. Isolates were obtained from plants exhibiting wilt symptoms in a protected cultivation system and in an open field. The specific primers 759/760 confirmed the isolates as part of the Ralstonia solanacearum complex, and Nmult primers were used to identify the phylotype. Variability analysis using BOX-PCR with the BOX-A1R primer on 19 isolates revealed molecular diversity. All 26 isolates were confirmed as Ralstonia solanacearum, belonging to phylotype II. Comparison of genomic DNA band patterns amplified by BOX-PCR indicated molecular variability, forming eight groups at a similarity level of 0.63. These results confirm the prevalence of R. solanacearum phylotype II in southwestern Paraná. This information aids decision-making in disease management and contributes to breeding efforts aiming at the development of resistant cultivars.
{"title":"Molecular identification of the causal agent of bacterial wilt in tomato plants in southwestern Paraná","authors":"Vanessa Casiraghi Zanon, J. M. Kafer, Jaqueline Hagn, Jéssica Cardoso, R. Dallemole-Giaretta, Taciane Finatto, T. Vargas","doi":"10.5433/1679-0359.2023v44n6p2249","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2249","url":null,"abstract":"Bacterial wilt, caused by phytopathogenic species of the genus Ralstonia, is one of the main diseases affecting the tomato crop. The Ralstonia solanacearum species complex occurs as a result of variants being widely diverse in terms of adaptation to different climatic conditions, host variations, and aggressiveness, which complicate disease control recommendations. In this study, we employed molecular methods to analyze 26 R. solanacearum isolates collected from tomato plants cultivated in the southwest region of Paraná, Brazil. Isolates were obtained from plants exhibiting wilt symptoms in a protected cultivation system and in an open field. The specific primers 759/760 confirmed the isolates as part of the Ralstonia solanacearum complex, and Nmult primers were used to identify the phylotype. Variability analysis using BOX-PCR with the BOX-A1R primer on 19 isolates revealed molecular diversity. All 26 isolates were confirmed as Ralstonia solanacearum, belonging to phylotype II. Comparison of genomic DNA band patterns amplified by BOX-PCR indicated molecular variability, forming eight groups at a similarity level of 0.63. These results confirm the prevalence of R. solanacearum phylotype II in southwestern Paraná. This information aids decision-making in disease management and contributes to breeding efforts aiming at the development of resistant cultivars.\u0000 ","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140479860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2197
Jaíne S.P. Vasconcellos, F. Fernandes, Carlos F. Barboza da Silva, Ê. Giotto, Sônia de Avila Botton, F. Vogel, Luis Antonio Sangioni
O georreferenciamento pode revelar a distribuição espacial das doenças. Este estudo caracterizou a localização geográfica dos casos positivos de leishmaniose visceral canina (LVC) (n=21), por meio do aplicativo C7-LVC (App), na cidade de Santa Maria, RS, Brasil. Esta tecnologia está disponível para uso em dispositivos smartphones, sendo caracterizada como primeiro instrumento para a notificação de LVC ao serviço público, com o objetivo de auxiliar nas ações de controle da doença. Neste estudo, foi utilizado o banco de dados do Serviço Municipal de Vigilância Ambiental que continha informações de notificações sobre LVC anteriormente enviadas por médicos veterinários, entre abril e dezembro de 2017. Neste período, a prevalência de LVC foi maior na região Norte (17/21 - 80,93%) da cidade. Os casos positivos foram observados em sete bairros, com maior ocorrência (21/9 - 42,85%) no bairro Perpétuo Socorro. As regiões contendo cães positivos para LVC apresentavam como características ambientais a vegetação nativa com áreas úmidas, sombreadas e ricas em matéria orgânica. Todos os casos de LVC foram identificados em regiões geográficas próximas. As características ambientais do município favorecem a proliferação e sobrevivência do vetor no perímetro urbano e periurbano e acarretam riscos à saúde humana e animal. Os dados de georreferenciamento obtidos pelo C7-LVC podem auxiliar na formulação de medidas sanitárias para conter a propagação da doença entre humanos e animais.
{"title":"Uso do aplicativo C7-LVC no georreferenciamento de casos de leishmaniose visceral canina","authors":"Jaíne S.P. Vasconcellos, F. Fernandes, Carlos F. Barboza da Silva, Ê. Giotto, Sônia de Avila Botton, F. Vogel, Luis Antonio Sangioni","doi":"10.5433/1679-0359.2023v44n6p2197","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2197","url":null,"abstract":"O georreferenciamento pode revelar a distribuição espacial das doenças. Este estudo caracterizou a localização geográfica dos casos positivos de leishmaniose visceral canina (LVC) (n=21), por meio do aplicativo C7-LVC (App), na cidade de Santa Maria, RS, Brasil. Esta tecnologia está disponível para uso em dispositivos smartphones, sendo caracterizada como primeiro instrumento para a notificação de LVC ao serviço público, com o objetivo de auxiliar nas ações de controle da doença. Neste estudo, foi utilizado o banco de dados do Serviço Municipal de Vigilância Ambiental que continha informações de notificações sobre LVC anteriormente enviadas por médicos veterinários, entre abril e dezembro de 2017. Neste período, a prevalência de LVC foi maior na região Norte (17/21 - 80,93%) da cidade. Os casos positivos foram observados em sete bairros, com maior ocorrência (21/9 - 42,85%) no bairro Perpétuo Socorro. As regiões contendo cães positivos para LVC apresentavam como características ambientais a vegetação nativa com áreas úmidas, sombreadas e ricas em matéria orgânica. Todos os casos de LVC foram identificados em regiões geográficas próximas. As características ambientais do município favorecem a proliferação e sobrevivência do vetor no perímetro urbano e periurbano e acarretam riscos à saúde humana e animal. Os dados de georreferenciamento obtidos pelo C7-LVC podem auxiliar na formulação de medidas sanitárias para conter a propagação da doença entre humanos e animais.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140485870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.5433/1679-0359.2023v44n6p2147
G. S. Wenneck, R. Saath, Roberto Rezende, Daniele de Souza Terassi, Vinícius Villa e Vila, Karym Mayara de Oliveira, A. L. Moro, Paulo Sérgio Lourenço de Freitas
Water management has a direct impact on plant development, and under deficit conditions, it often results in reduced yields. Silicon (Si), however, has the potential to alleviate stress and enhance plant performance under unfavorable conditions. This study aimed to analyze the performance of tomato plants cultivated under different water replacement depths and forms of silicon application. The experiment was laid out in a completely randomized design with a 2 × 4 factorial arrangement represented by two water replacement depths (60% and 100% of crop evapotranspiration - ETc) and four forms of silicon application (without application, soil application - full dose, soil application - split dose, and foliar applications). Four replications were used. The plants were cultivated in a protected environment using drip irrigation for water replacement, and silicon oxide served as the source of the element. The analyzed parameters included daily evapotranspiration, leaf spectral reflectance, mass accumulation (root, stem, and leaf), yield indices (fruit weight, plant yield, and defective fruits), water productivity, and post-harvest fruit weight loss. The imposition of water deficit (60% of ETc) in tomato leads to reduced crop development and yield, with the effects partially mitigated by the application of silicon. Conversely, under conditions of adequate water replacement (100% of ETc), silicon application contributes to increased development and yield of tomato. The application of silicon in the soil, whether in a full or split dose, demonstrates a more favorable response in vegetative indices and yield for tomato.
{"title":"Performance of tomato grown under different water replacement depths and silicon application forms","authors":"G. S. Wenneck, R. Saath, Roberto Rezende, Daniele de Souza Terassi, Vinícius Villa e Vila, Karym Mayara de Oliveira, A. L. Moro, Paulo Sérgio Lourenço de Freitas","doi":"10.5433/1679-0359.2023v44n6p2147","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2147","url":null,"abstract":"Water management has a direct impact on plant development, and under deficit conditions, it often results in reduced yields. Silicon (Si), however, has the potential to alleviate stress and enhance plant performance under unfavorable conditions. This study aimed to analyze the performance of tomato plants cultivated under different water replacement depths and forms of silicon application. The experiment was laid out in a completely randomized design with a 2 × 4 factorial arrangement represented by two water replacement depths (60% and 100% of crop evapotranspiration - ETc) and four forms of silicon application (without application, soil application - full dose, soil application - split dose, and foliar applications). Four replications were used. The plants were cultivated in a protected environment using drip irrigation for water replacement, and silicon oxide served as the source of the element. The analyzed parameters included daily evapotranspiration, leaf spectral reflectance, mass accumulation (root, stem, and leaf), yield indices (fruit weight, plant yield, and defective fruits), water productivity, and post-harvest fruit weight loss. The imposition of water deficit (60% of ETc) in tomato leads to reduced crop development and yield, with the effects partially mitigated by the application of silicon. Conversely, under conditions of adequate water replacement (100% of ETc), silicon application contributes to increased development and yield of tomato. The application of silicon in the soil, whether in a full or split dose, demonstrates a more favorable response in vegetative indices and yield for tomato.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140482634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.5433/1679-0359.2023v44n6p2079
C. Reichen, A. Coldebella, Diogenes Dezen, M. Meneguzzi, C. Pissetti, J. D. Kich
Salmonella enterica can be carried by pigs and can reach the final product and the consumer. Thus, Salmonella reduction strategies along the swine production chain should be studied. In this sense, the objective of the study was to evaluate a subunit vaccine based on secondary antigens administered orally against natural infection in swine farms in Brazil. A field trial study was conducted to estimate the effect of the vaccination on the Salmonella spp. seroprevalence, presence in lymph nodes, and fecal content in commercial pig herds in a vertical integration system belonging to an agroindustry. Furthermore, nMPN, qPCR, and phagocytic activity were performed. There were no significant differences in seroprevalence between groups. The mMPN count of Salmonella spp. in feces was higher in vaccinated group (VG), ranging from 0 to 2.46 log mMPN/g, while in control group (CG) it ranged from 0.0 to 4 log mMPN/g, showing a significant group effect (p<0.05), being confirmed in the qPCR. The activity of phagocytic monocytes was not altered by vaccination on farms. Thus, the oral subunit vaccination strategy at this stage of development did not reduce the spread and amplification of the infection in farms that would impact the prevalence of pigs carrying and shedding Salmonella spp. until slaughter.
{"title":"Efficiency evaluation of a novel orally administered subunit vaccine to reduce the prevalence of Salmonella enterica in swine under field conditions","authors":"C. Reichen, A. Coldebella, Diogenes Dezen, M. Meneguzzi, C. Pissetti, J. D. Kich","doi":"10.5433/1679-0359.2023v44n6p2079","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2079","url":null,"abstract":"Salmonella enterica can be carried by pigs and can reach the final product and the consumer. Thus, Salmonella reduction strategies along the swine production chain should be studied. In this sense, the objective of the study was to evaluate a subunit vaccine based on secondary antigens administered orally against natural infection in swine farms in Brazil. A field trial study was conducted to estimate the effect of the vaccination on the Salmonella spp. seroprevalence, presence in lymph nodes, and fecal content in commercial pig herds in a vertical integration system belonging to an agroindustry. Furthermore, nMPN, qPCR, and phagocytic activity were performed. There were no significant differences in seroprevalence between groups. The mMPN count of Salmonella spp. in feces was higher in vaccinated group (VG), ranging from 0 to 2.46 log mMPN/g, while in control group (CG) it ranged from 0.0 to 4 log mMPN/g, showing a significant group effect (p<0.05), being confirmed in the qPCR. The activity of phagocytic monocytes was not altered by vaccination on farms. Thus, the oral subunit vaccination strategy at this stage of development did not reduce the spread and amplification of the infection in farms that would impact the prevalence of pigs carrying and shedding Salmonella spp. until slaughter.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138962998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-18DOI: 10.5433/1679-0359.2023v44n6p2011
Joelson Arruda Jardim, Marcela Fernanda Moretti, Alexandre Lima de Andrade
The purpose of this study was to evaluate changes in the conjunctival and nasal microbiota, in the long term, of dogs undergoing bilateral dacryocystorhinostomy. Twelve male and female dogs (23 eyes), aged between 1 and 10 years, were enrolled in the study, selected on the basis of presentation with epiphora and chromodacryorrhea for at least six months. Cultures of material obtained from the ocular conjunctiva and nasal sinus of all dogs were evaluated to determine the conjunctival and nasal microbiota pre-and postoperatively (at 60, 120, and 240 d). Preoperatively, gram-negative bacteria were identified in the conjunctival microbiota of 66.5% (n=8), while gram-positive bacteria were found in 33.3% (n=4). Throughout the clinical evolution, a balance was found between the presence of gram-positive and gram-negative bacteria in the conjunctival microbiota. Pure cultures of Pseudomonas aeruginosa (25%) and Staphylococcus intermedius (25%) were found most frequently. Regarding the conjunctival microbiota, we can conclude that in obstructive diseases, there is a predominance of gram-negative bacteria in the lacrimal system, notably Pseudomonas aeruginosa. This study did not observe an increase in bacterial counts in the nasal cavity through the new surgical pathway to the conjunctival sac.
{"title":"Conjunctival and nasal microbiota evaluation of dogs submitted to dacryocystorhinostomy","authors":"Joelson Arruda Jardim, Marcela Fernanda Moretti, Alexandre Lima de Andrade","doi":"10.5433/1679-0359.2023v44n6p2011","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p2011","url":null,"abstract":"The purpose of this study was to evaluate changes in the conjunctival and nasal microbiota, in the long term, of dogs undergoing bilateral dacryocystorhinostomy. Twelve male and female dogs (23 eyes), aged between 1 and 10 years, were enrolled in the study, selected on the basis of presentation with epiphora and chromodacryorrhea for at least six months. Cultures of material obtained from the ocular conjunctiva and nasal sinus of all dogs were evaluated to determine the conjunctival and nasal microbiota pre-and postoperatively (at 60, 120, and 240 d). Preoperatively, gram-negative bacteria were identified in the conjunctival microbiota of 66.5% (n=8), while gram-positive bacteria were found in 33.3% (n=4). Throughout the clinical evolution, a balance was found between the presence of gram-positive and gram-negative bacteria in the conjunctival microbiota. Pure cultures of Pseudomonas aeruginosa (25%) and Staphylococcus intermedius (25%) were found most frequently. Regarding the conjunctival microbiota, we can conclude that in obstructive diseases, there is a predominance of gram-negative bacteria in the lacrimal system, notably Pseudomonas aeruginosa. This study did not observe an increase in bacterial counts in the nasal cavity through the new surgical pathway to the conjunctival sac.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-18DOI: 10.5433/1679-0359.2023v44n6p1991
Valéria da Silva Guimarães, Regina Lucia dos Santos Silva, Ricássio de Souza Barberino, Istefani Moreira Mota, Joisyleide Gonçalves Costa Pinto, Maria Lilian Gomes Loiola Torres, Naiane Darklei do Santos Silva, Mário Adriano Ávila Queiroz, Maria Helena Tavares de Matos, Alane Pains Oliveira do Monte
This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes.
{"title":"Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles","authors":"Valéria da Silva Guimarães, Regina Lucia dos Santos Silva, Ricássio de Souza Barberino, Istefani Moreira Mota, Joisyleide Gonçalves Costa Pinto, Maria Lilian Gomes Loiola Torres, Naiane Darklei do Santos Silva, Mário Adriano Ávila Queiroz, Maria Helena Tavares de Matos, Alane Pains Oliveira do Monte","doi":"10.5433/1679-0359.2023v44n6p1991","DOIUrl":"https://doi.org/10.5433/1679-0359.2023v44n6p1991","url":null,"abstract":"This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes.","PeriodicalId":21921,"journal":{"name":"Semina: Ciências Agrárias","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138963739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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