Pub Date : 2019-05-31DOI: 10.15578/SQUALEN.V14I1.381
T. Suryaningrum, D. Ikasari
Tuna fishing in Ambon is mostly conducted by small scale fishers using small boats (1.5 GT) and traditional fishing gear. The caught tuna was loined on board and collected at landing post or mini-plant before being sent to an UPI in Ambon. The study was aimed to evaluate the handling of tuna loin at small scale fishers during processing frozen tuna loin product. Two forms of tuna loin i.e skin-on and the skin-less tuna loin, obtained from the fishers in Latuhalat and Tial districts in Ambon and than was stored in ice overnight. Tuna loin was sent to Fish Processing Unit and cleaned from the skin (for skin-on tuna loins) or trimmed (for the skin-less tuna loins). Then, tuna loins were treated with CO, wrapped with styrofoam paper, packed with plastic and incubated in chilling room at 1.12oC for 2 days until the tuna meat color becomes reddish. The tuna loins were then frozen using Air Blast Freezer (temperature of -35oC) for 8 hours. Observations were performed on the physical (temperature) and chemical properties (proximate analysis, pH and TVB), microbial contaminants (TPC, E coli and Salmonella) as well as the sensory properties of tuna loin at each processing steps, starting from when the tuna loin was landed and handled at the collecting level, after stored in ice overnight before CO treatment, after treated with CO and incubated in chilling room for 2 days before frozen tuna loin products. The study showed that the existing tuna handling during processing and frozen of frozen tuna loins tended to cause quality decrease of the tuna loins. Tuna loins in the form of skin-less produced better quality compared to skin-on tuna loins. The quality decrease of tuna loin occured rapidly during incubation process after treated with CO, showed by the decrease of TVB and organoleptic values as well as the increase of bacterial content. However, the frozen tuna loin products were still meeting the requirements for frozen tuna based on Indonesia National Standard 01.2346-2006.
{"title":"Evaluation On Tuna Loin Handling As Raw Materials To Improve The Quality Of Frozen Tuna Loin in Ambon","authors":"T. Suryaningrum, D. Ikasari","doi":"10.15578/SQUALEN.V14I1.381","DOIUrl":"https://doi.org/10.15578/SQUALEN.V14I1.381","url":null,"abstract":"Tuna fishing in Ambon is mostly conducted by small scale fishers using small boats (1.5 GT) and traditional fishing gear. The caught tuna was loined on board and collected at landing post or mini-plant before being sent to an UPI in Ambon. The study was aimed to evaluate the handling of tuna loin at small scale fishers during processing frozen tuna loin product. Two forms of tuna loin i.e skin-on and the skin-less tuna loin, obtained from the fishers in Latuhalat and Tial districts in Ambon and than was stored in ice overnight. Tuna loin was sent to Fish Processing Unit and cleaned from the skin (for skin-on tuna loins) or trimmed (for the skin-less tuna loins). Then, tuna loins were treated with CO, wrapped with styrofoam paper, packed with plastic and incubated in chilling room at 1.12oC for 2 days until the tuna meat color becomes reddish. The tuna loins were then frozen using Air Blast Freezer (temperature of -35oC) for 8 hours. Observations were performed on the physical (temperature) and chemical properties (proximate analysis, pH and TVB), microbial contaminants (TPC, E coli and Salmonella) as well as the sensory properties of tuna loin at each processing steps, starting from when the tuna loin was landed and handled at the collecting level, after stored in ice overnight before CO treatment, after treated with CO and incubated in chilling room for 2 days before frozen tuna loin products. The study showed that the existing tuna handling during processing and frozen of frozen tuna loins tended to cause quality decrease of the tuna loins. Tuna loins in the form of skin-less produced better quality compared to skin-on tuna loins. The quality decrease of tuna loin occured rapidly during incubation process after treated with CO, showed by the decrease of TVB and organoleptic values as well as the increase of bacterial content. However, the frozen tuna loin products were still meeting the requirements for frozen tuna based on Indonesia National Standard 01.2346-2006.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82179017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.15578/SQUALEN.V13I3.355
S. Sihono, K. Tarman, H. Madduppa, H. Januar
Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K10, K11, K12, and K13), while seawater handling extract contained very polar (K1, K2 and K3), polar (K6, K7, and K8) and low polar (K13) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC50 below 2,000 ppm) in ethanolic extract of C. racemosa. Keywords: antioxidant activity, Caulerpa racemosa, ethanolic extract,handlings, IC50
{"title":"Metabolite Profiles and Antioxidant Activity of Caulerpa racemosa with Different Handlings","authors":"S. Sihono, K. Tarman, H. Madduppa, H. Januar","doi":"10.15578/SQUALEN.V13I3.355","DOIUrl":"https://doi.org/10.15578/SQUALEN.V13I3.355","url":null,"abstract":" Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K10, K11, K12, and K13), while seawater handling extract contained very polar (K1, K2 and K3), polar (K6, K7, and K8) and low polar (K13) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC50 below 2,000 ppm) in ethanolic extract of C. racemosa. Keywords: antioxidant activity, Caulerpa racemosa, ethanolic extract,handlings, IC50","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79135767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.15578/SQUALEN.V13I3.292
I. Munifah, H. Irianto
Agar processed from red seaweed Gracilaria sp. in Indonesia can be found in the form of sheet and powder. The abundance of cellulose in agar solid waste can be used as an alternative source of carbon for microorganism growth. The purpose of this study was to determine the component of agar solid waste and to characterize the cellulose. The agar solid waste (limbah industri agar-agar, LIA) was undergone physical separation process into agar, fiber cellulose, and celite. The result showed that agar solid waste consisted of 53.53% fiber; 37.33% agar and 8.60% celite. LIA was characterized for its components including ash, lignin, extractive substances, cellulose, hemicellulose, and holocellulose using Technical Association of the Pulp and Paper Industry Method (TAPPI). The TAPPI analysis revealed that solid waste generated from seaweed Gracilaria sp processing had 28.19% cellulose, 38.83% holocellulosa, 10.63% hemicellulose, 8.27% ash, 3.54% insoluble acid ash, 11.23% water, and 1.62% extractives substances. The lignin content of the solid waste was low (2.08%), therefore it has potential to be utilized as biomass (bio fertilizer, alternative carbon source). The components in solid waste of agar was determined using Fourier Transform Infra Red (FTIR). The LIA sample had high content of celite indicated by the absorption peak which appears at wave length 2343.45 cm-1 for the -Si-H bond and at the wave length 772.99 cm-1 for the bond -Si- O-. Infra-red spectra showed that celite still exist in solid waste of agar. The study indicated that there was still a large amount of cellulose in the solid waste of agar. Keywords: solid waste, cellulose, agar, lignin, celite
从印度尼西亚的红紫菜中加工的琼脂可以以片状和粉末的形式找到。琼脂固体废物中丰富的纤维素可以作为微生物生长的替代碳源。本研究的目的是确定琼脂固体废物的成分，并对纤维素进行表征。将琼脂固体废弃物(limbah industrii agar-agar -agar, LIA)进行物理分离，得到琼脂、纤维纤维素和celite。结果表明:琼脂固体废弃物中纤维含量为53.53%;琼脂37.33%，青石8.60%。利用纸浆造纸工业技术协会方法(TAPPI)对LIA的组分进行了表征，包括灰分、木质素、萃取物、纤维素、半纤维素和全新纤维素。TAPPI分析表明，紫菜加工产生的固体废弃物中纤维素含量为28.19%，总纤维素含量为38.83%，半纤维素含量为10.63%，灰分含量为8.27%，不溶性酸灰分含量为3.54%，水含量为11.23%，萃取物含量为1.62%。固体废弃物中木质素含量较低(2.08%)，具有作为生物质(生物肥料、替代碳源)利用的潜力。采用傅里叶变换红外光谱(FTIR)对琼脂固体废物中的成分进行了测定。在- si - h键和- si - O-键的吸收峰波长分别为2343.45 cm-1和772.99 cm-1，表明LIA样品中青石的含量较高。红外光谱分析表明，琼脂固体废弃物中仍存在青石。研究表明，琼脂固体废弃物中仍存在大量的纤维素。关键词:固体废物，纤维素，琼脂，木质素，天青石
{"title":"Characteristics of Solid Waste Agar Industries","authors":"I. Munifah, H. Irianto","doi":"10.15578/SQUALEN.V13I3.292","DOIUrl":"https://doi.org/10.15578/SQUALEN.V13I3.292","url":null,"abstract":"Agar processed from red seaweed Gracilaria sp. in Indonesia can be found in the form of sheet and powder. The abundance of cellulose in agar solid waste can be used as an alternative source of carbon for microorganism growth. The purpose of this study was to determine the component of agar solid waste and to characterize the cellulose. The agar solid waste (limbah industri agar-agar, LIA) was undergone physical separation process into agar, fiber cellulose, and celite. The result showed that agar solid waste consisted of 53.53% fiber; 37.33% agar and 8.60% celite. LIA was characterized for its components including ash, lignin, extractive substances, cellulose, hemicellulose, and holocellulose using Technical Association of the Pulp and Paper Industry Method (TAPPI). The TAPPI analysis revealed that solid waste generated from seaweed Gracilaria sp processing had 28.19% cellulose, 38.83% holocellulosa, 10.63% hemicellulose, 8.27% ash, 3.54% insoluble acid ash, 11.23% water, and 1.62% extractives substances. The lignin content of the solid waste was low (2.08%), therefore it has potential to be utilized as biomass (bio fertilizer, alternative carbon source). The components in solid waste of agar was determined using Fourier Transform Infra Red (FTIR). The LIA sample had high content of celite indicated by the absorption peak which appears at wave length 2343.45 cm-1 for the -Si-H bond and at the wave length 772.99 cm-1 for the bond -Si- O-. Infra-red spectra showed that celite still exist in solid waste of agar. The study indicated that there was still a large amount of cellulose in the solid waste of agar. Keywords: solid waste, cellulose, agar, lignin, celite","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87584102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.15578/SQUALEN.V13I3.365
E. Marraskuranto, T. Raharjo, R. Kasiamdari, T. R. Nuringtyas
Microalgae is a photoautotroph organism capable of producing various photosynthetic pigments with diverse beneficial properties. Rhodomonas salina, a Cryptophyte cell, contains only phycoerythrin as its phycobiliprotein pigment. The effects of salinity on growth and phycoerythrin concentration were investigated. Microalgae R. salina were grown in natural sea water with salinity of 33‰ and 50‰.The microalgae was batch-cultured in f/2 medium at light irradiation of 1100 lux, temperature of 24–26 oC, and photoperiode of 12 h : 12 h. The microalgae cell density was directly calculated using haemacytometer. The concentration of phycoerythrin was determined by spectrophotometric method. The cell density and phycoerythrin concentration were monitored every 4 days for 20 days of cell growth. Results showed that salinity did not affect significantly both on growth and phycoerythrin concentration extracted from R. salina biomass (p>0.05; a = 0.05). At both salinity, maximum phycoerythrin concentration were reached on day 8. There was a positive correlation between cell density and phycoerythrin concentration from day 1 to day 8 of cell growth. Microalgae R. salina which was grown in natural seawater with salinity of 33‰ achieved the highest cell density of 8.4 x 105 cells/mL and the phycoerythrin concentration of 0.19 mg. 10-5 cell on day 8 of the culture. The highest phycoerythrin concentration was obtained on day 16 of the culture i.e 0.27 mg. 10-5 cell.Keywords: cell density, growth media, phycoerythrin, Rhodomonas salina, salinity
{"title":"Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina","authors":"E. Marraskuranto, T. Raharjo, R. Kasiamdari, T. R. Nuringtyas","doi":"10.15578/SQUALEN.V13I3.365","DOIUrl":"https://doi.org/10.15578/SQUALEN.V13I3.365","url":null,"abstract":"Microalgae is a photoautotroph organism capable of producing various photosynthetic pigments with diverse beneficial properties. Rhodomonas salina, a Cryptophyte cell, contains only phycoerythrin as its phycobiliprotein pigment. The effects of salinity on growth and phycoerythrin concentration were investigated. Microalgae R. salina were grown in natural sea water with salinity of 33‰ and 50‰.The microalgae was batch-cultured in f/2 medium at light irradiation of 1100 lux, temperature of 24–26 oC, and photoperiode of 12 h : 12 h. The microalgae cell density was directly calculated using haemacytometer. The concentration of phycoerythrin was determined by spectrophotometric method. The cell density and phycoerythrin concentration were monitored every 4 days for 20 days of cell growth. Results showed that salinity did not affect significantly both on growth and phycoerythrin concentration extracted from R. salina biomass (p>0.05; a = 0.05). At both salinity, maximum phycoerythrin concentration were reached on day 8. There was a positive correlation between cell density and phycoerythrin concentration from day 1 to day 8 of cell growth. Microalgae R. salina which was grown in natural seawater with salinity of 33‰ achieved the highest cell density of 8.4 x 105 cells/mL and the phycoerythrin concentration of 0.19 mg. 10-5 cell on day 8 of the culture. The highest phycoerythrin concentration was obtained on day 16 of the culture i.e 0.27 mg. 10-5 cell.Keywords: cell density, growth media, phycoerythrin, Rhodomonas salina, salinity","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81841610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.15578/SQUALEN.V13I3.370
D. Dwiyitno, Stefan Hoffman, Koen Parmentier, C. V. Keer
Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen
{"title":"Method Comparison of DNA Isolation and Quantification for Fish and Seafood Authenticity Determination","authors":"D. Dwiyitno, Stefan Hoffman, Koen Parmentier, C. V. Keer","doi":"10.15578/SQUALEN.V13I3.370","DOIUrl":"https://doi.org/10.15578/SQUALEN.V13I3.370","url":null,"abstract":"Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91096022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.15578/SQUALEN.V13I3.367
D. S. Zilda, Y. N. Fawzya, A. Uria
Proteases or peptidases is known as a largest group of hydrolytic enzymes and have been applied in various industries such as food, pharmacy, leather, detergent and waste treatment. Although they are also produced by plants and animals, microbes remain the main source of proteases in the world market which mostly derived from Bacillus sp. Aims of this research were to identify isolate BII-1 and study its protease. Analysis of 16Sr RNA sequencing showed the identity of BII-1 as Bacillus subtilis (99% similarity with the same species in GenBank). It was found that protease from BII-1 exhibited optimal temperature and pH of 50 oC and 8-9, respectively. It was activated by Li2+, Na2+, Mg2+ and K+. The degenerated primer for protease gene was designed, and a partial protease gene was amplified from BII-1. The sequencing result showed that this amplified gene shared 100 and 99% similarity with those from Geobacillus thermophiles and Bacillus subtilis in the GenBank, respectively.Keywords: protease, bacteria, Bacillus subtilis, Geobacillus thermophylus
{"title":"Identification of Protease-Producing Bacteria Isolated from Banyuwedang, Bali, and Characterization of its Protease","authors":"D. S. Zilda, Y. N. Fawzya, A. Uria","doi":"10.15578/SQUALEN.V13I3.367","DOIUrl":"https://doi.org/10.15578/SQUALEN.V13I3.367","url":null,"abstract":"Proteases or peptidases is known as a largest group of hydrolytic enzymes and have been applied in various industries such as food, pharmacy, leather, detergent and waste treatment. Although they are also produced by plants and animals, microbes remain the main source of proteases in the world market which mostly derived from Bacillus sp. Aims of this research were to identify isolate BII-1 and study its protease. Analysis of 16Sr RNA sequencing showed the identity of BII-1 as Bacillus subtilis (99% similarity with the same species in GenBank). It was found that protease from BII-1 exhibited optimal temperature and pH of 50 oC and 8-9, respectively. It was activated by Li2+, Na2+, Mg2+ and K+. The degenerated primer for protease gene was designed, and a partial protease gene was amplified from BII-1. The sequencing result showed that this amplified gene shared 100 and 99% similarity with those from Geobacillus thermophiles and Bacillus subtilis in the GenBank, respectively.Keywords: protease, bacteria, Bacillus subtilis, Geobacillus thermophylus","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74258661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-31DOI: 10.15578/squalen.v13i2.356
I. Hidayah
This study aims to determine the probability of Aflatoxin B1 exposure from Aspergillus flavus in dried salted fish. The exposure of that may cause a health problems to consumers. The collecting of salted fish was conducted in several areas in Java Island, which were Tangerang, Bandung, Cirebon, Pelabuhan Ratu, Tegal, Cilacap, Banyuwangi and Tuban. Isolation and identification of Aspergillus flavus was conducted by using pour plate method on Aspergillus Flavus Parasiticus Agar (AFPA) media. Meanwhile, Measurement of Aflatoxin B1 (AFB1) content had been done by enzyme linked immunosorbent assay (ELISA). On the other hand, the occurence probability of Aflatoxin B1 risk from Aspergillus flavus was calculated by statistical probabilistic approach in @risk version 7.0 software with Monte Carlo simulation. The results of this study showed that in the consumption of salted fish for about 3.7 g/capita/day, it is probable that there are risk of 7.74 cfu/g A. flavus exposure and 0.7291 ppb aflatoxin B1 exposure in 1 g of salted fish that were taken from sampling locations. This value is still catagorized as low risk level
本研究旨在确定咸鱼干中黄曲霉暴露黄曲霉毒素B1的可能性。接触到这些物质可能会给消费者带来健康问题。在爪哇岛的几个地区进行了咸鱼的采集工作，这些地区是:坦格朗、万隆、奇勒本、佩拉布汉拉图、特加尔、奇拉卡普、班育旺吉和图班。采用倒平板法在黄曲霉寄生琼脂(AFPA)培养基上对黄曲霉进行分离鉴定。同时，采用酶联免疫吸附法(ELISA)测定黄曲霉毒素B1 (AFB1)含量。另一方面，在@risk version 7.0软件中，通过蒙特卡罗模拟，采用统计概率法计算黄曲霉B1风险的发生概率。本研究结果表明，人均每天食用约3.7 g的咸鱼，每g取样地点的咸鱼可能有7.74 cfu/g黄曲霉毒素暴露风险和0.7291 ppb黄曲霉毒素B1暴露风险。这个值仍然被归类为低风险级别
{"title":"SEMI QUANTITATIVE RISK PROFILE OF AFLATOXIN B1 FROM AN ASPERGILLUS FLAVUS IN A DRIED SALTED FISH IN JAVA ISLAND RESELLERS","authors":"I. Hidayah","doi":"10.15578/squalen.v13i2.356","DOIUrl":"https://doi.org/10.15578/squalen.v13i2.356","url":null,"abstract":"This study aims to determine the probability of Aflatoxin B1 exposure from Aspergillus flavus in dried salted fish. The exposure of that may cause a health problems to consumers. The collecting of salted fish was conducted in several areas in Java Island, which were Tangerang, Bandung, Cirebon, Pelabuhan Ratu, Tegal, Cilacap, Banyuwangi and Tuban. Isolation and identification of Aspergillus flavus was conducted by using pour plate method on Aspergillus Flavus Parasiticus Agar (AFPA) media. Meanwhile, Measurement of Aflatoxin B1 (AFB1) content had been done by enzyme linked immunosorbent assay (ELISA). On the other hand, the occurence probability of Aflatoxin B1 risk from Aspergillus flavus was calculated by statistical probabilistic approach in @risk version 7.0 software with Monte Carlo simulation. The results of this study showed that in the consumption of salted fish for about 3.7 g/capita/day, it is probable that there are risk of 7.74 cfu/g A. flavus exposure and 0.7291 ppb aflatoxin B1 exposure in 1 g of salted fish that were taken from sampling locations. This value is still catagorized as low risk level","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78545805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-31DOI: 10.15578/squalen.v13i2.354
Ajeng Kurniasari Putri, Umi Anissah, F. Ariyani, S. Wibowo
Opah fish(Lampris guttatus) is one of the bycatch products of Tuna fish captured originally from Indonesia that currently has become as one of the exported commodities. However, it is stated that these fish contains high formaldehyde up to 200 ppm, which is strongly suspected naturally due to deterioration. Furthermore, the aim of this study is to obtain the data of probabilistic health risk assessment due to consumption of opah fish that contaminated with natural formaldehyde. The study was conducted on opah fish (Lampris guttatus) that were analyzed the formaldehyde concentration in it. Along with the consumption data, body weight and the formaldehyde concentration included two others simulations of two times and four times of formaldehyde value, probabilistic dietary exposure was calculated by @Risk and produced some data regard to health risk. The result showed that Opah fish caught in Indonesian waters could produce formaldehyde naturally due to deterioration process ranged from 4,62 ± 0,00 mg/kg to 58,10 ± 0,46 mg/kg. Consequently, the residents of female children in Jakarta and Surabaya considered as in health risk problems. Extremely, the further simulations of two times and four times of formaldehyde concentration showed the health risk to all residents in Jakarta and Surabaya included male, female, children, and adult. Therefore, the stakeholders included government and policymakers should take some priorities to formulating a proper risk management strategy on the basis of knowledge of endogenous formaldehyde present in Opah fish and risk management strategies for the fish consumer in Indonesia.
{"title":"Probabilistic Health Risk Assessment Due to Natural Formaldehyde Intake through Opah Fish (Lampris guttatus) Consumption in Indonesia","authors":"Ajeng Kurniasari Putri, Umi Anissah, F. Ariyani, S. Wibowo","doi":"10.15578/squalen.v13i2.354","DOIUrl":"https://doi.org/10.15578/squalen.v13i2.354","url":null,"abstract":"Opah fish(Lampris guttatus) is one of the bycatch products of Tuna fish captured originally from Indonesia that currently has become as one of the exported commodities. However, it is stated that these fish contains high formaldehyde up to 200 ppm, which is strongly suspected naturally due to deterioration. Furthermore, the aim of this study is to obtain the data of probabilistic health risk assessment due to consumption of opah fish that contaminated with natural formaldehyde. The study was conducted on opah fish (Lampris guttatus) that were analyzed the formaldehyde concentration in it. Along with the consumption data, body weight and the formaldehyde concentration included two others simulations of two times and four times of formaldehyde value, probabilistic dietary exposure was calculated by @Risk and produced some data regard to health risk. The result showed that Opah fish caught in Indonesian waters could produce formaldehyde naturally due to deterioration process ranged from 4,62 ± 0,00 mg/kg to 58,10 ± 0,46 mg/kg. Consequently, the residents of female children in Jakarta and Surabaya considered as in health risk problems. Extremely, the further simulations of two times and four times of formaldehyde concentration showed the health risk to all residents in Jakarta and Surabaya included male, female, children, and adult. Therefore, the stakeholders included government and policymakers should take some priorities to formulating a proper risk management strategy on the basis of knowledge of endogenous formaldehyde present in Opah fish and risk management strategies for the fish consumer in Indonesia.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86676281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-31DOI: 10.15578/SQUALEN.V13I2.345
S. Budiari, E. Chasanah, M. Suhartono, N. Palupi
The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead meat is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous bioactive protein or peptide from snakehead meat and to fractionate the active compounds using ultrafiltration. The extraction in this research employs two kinds of solvents, e.i aquadest and 50% ethanol. Then, the extract is fractionated with the ultrafiltration using the 10,000; 5,000 and 3,000 MWCO membranes to separate the protein or peptide of with the sizes of >10 kDa, 5 – 10 kDa, 3 – 5 kDa and <3 kDa. The parameters being observed from the crude extracts and its fractions included the protein and peptide content, the ACE inhibitory activity (in vitro), and the protein and peptide profiles determined by using the SDS PAGE. The result of this research revealed that the snakehead meat contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide sized <10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of >10 kDa than the 50% ethanol was. The bioactivity of 50% ethanol extraction was higher than the aquadest extraction. The highest bioactivity of 95.18% was gained from the fraction 5 – 10 kDa of 50% ethanol extract
{"title":"ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY OF CRUDE AND FRACTIONATED SNAKEHEAD (Channa striata) MEAT EXTRACT","authors":"S. Budiari, E. Chasanah, M. Suhartono, N. Palupi","doi":"10.15578/SQUALEN.V13I2.345","DOIUrl":"https://doi.org/10.15578/SQUALEN.V13I2.345","url":null,"abstract":"The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead meat is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous bioactive protein or peptide from snakehead meat and to fractionate the active compounds using ultrafiltration. The extraction in this research employs two kinds of solvents, e.i aquadest and 50% ethanol. Then, the extract is fractionated with the ultrafiltration using the 10,000; 5,000 and 3,000 MWCO membranes to separate the protein or peptide of with the sizes of >10 kDa, 5 – 10 kDa, 3 – 5 kDa and <3 kDa. The parameters being observed from the crude extracts and its fractions included the protein and peptide content, the ACE inhibitory activity (in vitro), and the protein and peptide profiles determined by using the SDS PAGE. The result of this research revealed that the snakehead meat contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide sized <10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of >10 kDa than the 50% ethanol was. The bioactivity of 50% ethanol extraction was higher than the aquadest extraction. The highest bioactivity of 95.18% was gained from the fraction 5 – 10 kDa of 50% ethanol extract","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79164170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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