Shark (Prionace glauca) cartilage is an industrial waste of the frozen fish industry in Sidoarjo city. Previous studies indicate that the cartilage contains bioactive compounds, glucosamine, and chondroitin, but the extracted product is unstable (easily melted during storage) and smelly ammonia. This study aims to obtain a stable extract product without the smell of ammonia by adding different absorbers. The experiment used a Complete Randomized Design on applying absorbers, HPMC, Cab-Osil, and Avicel PH 101. The parameters measured were the yield and the organoleptic of the shark’s extract product. The microstructural observations used a Scanning Electron Microscope (SEM), while the chondroitin sulfate content analyses used high-performance liquid chromatography(HPLC). Results showed that adding different absorbers significantly influenced the yield and organoleptic of the shark’s cartilage extract. The use of Avicel PH 101 (90%), Cab-Osil (9%), and Hydroxy Propyl Methyl Cellulose (HPMC) (1%) gave the highest amount of extract, as much as 5.02 g (16.73%). The organoleptic was without smelling salts, whitish beige color, and dry structure (stable). The microstructure of the shark’s cartilage extract product at this treatment had a dense structure with a soft surface. In contrast, the extract without an absorber addition had a sharp, needle-like microstructure. The chondroitin sulfate content of the shark’s cartilage extract product was the highest at this treatment.
{"title":"The Microstructure and Potential of Chondroitin Sulfate in Shark Cartilage Extract","authors":"Titiek Indhira Agustin, Risma Risma, Retno Sari, Dwi Setyawan","doi":"10.15578/squalen.760","DOIUrl":"https://doi.org/10.15578/squalen.760","url":null,"abstract":"Shark (Prionace glauca) cartilage is an industrial waste of the frozen fish industry in Sidoarjo city. Previous studies indicate that the cartilage contains bioactive compounds, glucosamine, and chondroitin, but the extracted product is unstable (easily melted during storage) and smelly ammonia. This study aims to obtain a stable extract product without the smell of ammonia by adding different absorbers. The experiment used a Complete Randomized Design on applying absorbers, HPMC, Cab-Osil, and Avicel PH 101. The parameters measured were the yield and the organoleptic of the shark’s extract product. The microstructural observations used a Scanning Electron Microscope (SEM), while the chondroitin sulfate content analyses used high-performance liquid chromatography(HPLC). Results showed that adding different absorbers significantly influenced the yield and organoleptic of the shark’s cartilage extract. The use of Avicel PH 101 (90%), Cab-Osil (9%), and Hydroxy Propyl Methyl Cellulose (HPMC) (1%) gave the highest amount of extract, as much as 5.02 g (16.73%). The organoleptic was without smelling salts, whitish beige color, and dry structure (stable). The microstructure of the shark’s cartilage extract product at this treatment had a dense structure with a soft surface. In contrast, the extract without an absorber addition had a sharp, needle-like microstructure. The chondroitin sulfate content of the shark’s cartilage extract product was the highest at this treatment.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136036180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as a raw material for various natural products, food, and pharmaceutical industries. The main challenge regarding its production, escpecially in Tarakan is the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information and the cultivation performance of the selected seeds by identifying genetic sources based on molecular markers. DNA sequencing analysis was carried out using the molecular marker cox2-3 spacer on a collection comprising 16 cultivars from all over the country (Indonesia). The four haplotypes representing K. alvarezii to be cultivated in the city were produced by phylogenetic clustering and further molecular analysis using cox2-3 intergenic spacer mitochondrial DNA marker. These include the Kupang varieties and Natuna, also referred to as Lampung seedlings, which is a tissue culture plantlet used for development at SEAMEO-BIOTROP Bogor and propagated by the Lampung Marine Aquaculture Center (BBPBL), Tarakan. These varieties were genetically distances (pairwise comparisons based on mean Kimura 2-parameter model) ranging from 0.00-0.23 unit. The cultivation was carried out for 30 days in the waters of Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan, for 30 days. The result showed that the Natuna* as Lampung cultivar has superior performance compared to local cultivars (Takalar as Mamburungan Tanjung Batu, Tarakan, and Kupang). Furthermore, the genetic analysis with cox2-3 spacer markers had the potential as an effective seed selection tool for tracing genetic information in the development of superior seedlings in aquaculture activities.
Kappaphycus alvarezii海藻是一种重要的水产养殖商品，具有很高的经济价值，已被用作各种天然产品、食品和制药工业的原料。生产方面的主要挑战，特别是在塔拉干，是优质种子的供应。因此，本研究旨在通过基于分子标记的遗传来源鉴定，获得所选种子的系统发育信息和培养性能。利用分子标记cox2-3 spacer对来自全国(印度尼西亚)的16个品种进行了DNA测序分析。通过系统发育聚类和cox2-3基因间间隔线粒体DNA标记的分子分析，获得了代表我市拟栽培的4个单倍型。这些品种包括Kupang品种和Natuna，也被称为楠榜幼苗，这是一种组织培养苗，用于SEAMEO-BIOTROP茂物的发育，并由塔拉干的楠榜海洋水产养殖中心(BBPBL)繁殖。这些品种的遗传距离(基于平均Kimura 2参数模型的两两比较)在0.00-0.23单位之间。在丹戎巴图(Tj.)水域进行了30天的培养。在北加里曼丹的塔拉干(Tarakan)的Mamburungan逗留30天。结果表明，纳土纳*作为楠榜品种与当地品种(Takalar作为Mamburungan, Tanjung Batu, Tarakan和Kupang)相比具有优越的性能。此外，利用cox2-3间隔标记进行遗传分析，可作为一种有效的种子选择工具，在水产养殖活动中追踪优良苗的遗传信息。
{"title":"Molecular Assessment of Kappaphycus alvarezii Cultivated in Tarakan based on cox2-3 Spacer","authors":"D. Soelistyowati","doi":"10.15578/squalen.736","DOIUrl":"https://doi.org/10.15578/squalen.736","url":null,"abstract":"The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as a raw material for various natural products, food, and pharmaceutical industries. The main challenge regarding its production, escpecially in Tarakan is the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information and the cultivation performance of the selected seeds by identifying genetic sources based on molecular markers. DNA sequencing analysis was carried out using the molecular marker cox2-3 spacer on a collection comprising 16 cultivars from all over the country (Indonesia). The four haplotypes representing K. alvarezii to be cultivated in the city were produced by phylogenetic clustering and further molecular analysis using cox2-3 intergenic spacer mitochondrial DNA marker. These include the Kupang varieties and Natuna, also referred to as Lampung seedlings, which is a tissue culture plantlet used for development at SEAMEO-BIOTROP Bogor and propagated by the Lampung Marine Aquaculture Center (BBPBL), Tarakan. These varieties were genetically distances (pairwise comparisons based on mean Kimura 2-parameter model) ranging from 0.00-0.23 unit. The cultivation was carried out for 30 days in the waters of Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan, for 30 days. The result showed that the Natuna* as Lampung cultivar has superior performance compared to local cultivars (Takalar as Mamburungan Tanjung Batu, Tarakan, and Kupang). Furthermore, the genetic analysis with cox2-3 spacer markers had the potential as an effective seed selection tool for tracing genetic information in the development of superior seedlings in aquaculture activities.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72393269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Catfish is one of the freshwater aquaculture commodities with a high level of consumption and production in Indonesia. Disease outbreaks could occur in catfish farming activities caused by pathogenic bacteria. Several species of pathogenic bacteria can cause disease in catfish, resulting in mass death. This can lead to decrease in the food quality of freshwater fishery products, especially catfish. In cultured system, aquaculture occurrence of diseases can cause severe financial losses. Catfish samples were obtained from catfish farming with clinical symptoms of reddish spots on the outside of the body. Bacteria were isolated from the kidney and liver under aseptic conditions. These bacteria isolates were identified through their colony morphology, Gram staining, biochemical tests, molecular test, and antibacterial test of Bacillus spp. using spot and disc diffusion test. Identification based on 16S rRNA gene showed that GL1 was 99.92% closely related to Aeromonas widowei, HL1 was 100% closely to Bacillus amyloliquefaciens, and GL2 and HL2 was closely related to Bacillus cereus. The antibacterial test results of APD10 isolates of Bacillus velenzensis species inhibited GL2 pathogenic bacteria with an inhibition zone of 22.15 mm in the very strong inhibition zone and HL2 pathogenic bacteria with an inhibition zone of 8.5 mm in the moderate inhibition zone. Bacillus velezensis was isolated from the sponge Aplysina sp. could be further utilized as a biocontrol agent for the pathogenic bacteria, Bacillus cereus, that infects catfish.
{"title":"The Potential of Sponge-Associated Bacillus spp. as A Biocontrol Agent to Inhibit Several Bacteria from Infected Catfish (Clarias gariepinus Burch)","authors":"Della Indah Medani","doi":"10.15578/squalen.724","DOIUrl":"https://doi.org/10.15578/squalen.724","url":null,"abstract":"Catfish is one of the freshwater aquaculture commodities with a high level of consumption and production in Indonesia. Disease outbreaks could occur in catfish farming activities caused by pathogenic bacteria. Several species of pathogenic bacteria can cause disease in catfish, resulting in mass death. This can lead to decrease in the food quality of freshwater fishery products, especially catfish. In cultured system, aquaculture occurrence of diseases can cause severe financial losses. Catfish samples were obtained from catfish farming with clinical symptoms of reddish spots on the outside of the body. Bacteria were isolated from the kidney and liver under aseptic conditions. These bacteria isolates were identified through their colony morphology, Gram staining, biochemical tests, molecular test, and antibacterial test of Bacillus spp. using spot and disc diffusion test. Identification based on 16S rRNA gene showed that GL1 was 99.92% closely related to Aeromonas widowei, HL1 was 100% closely to Bacillus amyloliquefaciens, and GL2 and HL2 was closely related to Bacillus cereus. The antibacterial test results of APD10 isolates of Bacillus velenzensis species inhibited GL2 pathogenic bacteria with an inhibition zone of 22.15 mm in the very strong inhibition zone and HL2 pathogenic bacteria with an inhibition zone of 8.5 mm in the moderate inhibition zone. Bacillus velezensis was isolated from the sponge Aplysina sp. could be further utilized as a biocontrol agent for the pathogenic bacteria, Bacillus cereus, that infects catfish.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135394934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microplastic (MP) pollution is an emerging environmental problem that threatens food security, food safety, and human health since it has been reported to be found in commercial fish consumed by humans. Bitung, North Sulawesi, is one of the biggest contributors to capture fishery production in Indonesia. However, there is no data on microplastic pollution in commercial marine fish from Bitung. Therefore, this research aimed to investigate the presence and identify the visual characteristics (color, shape, size) and the polymer type of microplastics found in the gastrointestinal tract of commercial marine fish from Bitung, North Sulawesi. The gastrointestinal tract was extracted using KOH 10%, and the microplastic was observed under a stereo microscope. A total of 753 microplastic particles were found in the gastrointestinal tract of 74 individuals (prevalence 99%), and there was a statistically significant difference in the abundance of microplastics found in the gastrointestinal tract of pelagic and demersal fish. The average number of microplastic particles found in the gastrointestinal tract of pelagic fish (12,24 ± 2,43) is higher than in demersal fish (7,38 ± 3,48). The dominant color and shape of microplastic found in the gastrointestinal tract of the fish were black and fiber, respectively. At the same time, the dominant microplastic size found in the gastrointestinal tract of demersal fish was bigger (1,001-5,000 µm, 39,4%) compared to pelagic fish (150-500 µm, 47%). The Fourier Transform Spectroscopy (FTIR) analysis result shows that microplastics of the same polymer type can be found in the gastrointestinal tract of both pelagic and demersal fish.
{"title":"Abundance and Characteristics of Microplastics Found in The Gastrointestinal Tract of Commercial Marine Fish from Bitung, North Sulawesi – Indonesia","authors":"Aunurohim Aunurohim","doi":"10.15578/squalen.719","DOIUrl":"https://doi.org/10.15578/squalen.719","url":null,"abstract":"Microplastic (MP) pollution is an emerging environmental problem that threatens food security, food safety, and human health since it has been reported to be found in commercial fish consumed by humans. Bitung, North Sulawesi, is one of the biggest contributors to capture fishery production in Indonesia. However, there is no data on microplastic pollution in commercial marine fish from Bitung. Therefore, this research aimed to investigate the presence and identify the visual characteristics (color, shape, size) and the polymer type of microplastics found in the gastrointestinal tract of commercial marine fish from Bitung, North Sulawesi. The gastrointestinal tract was extracted using KOH 10%, and the microplastic was observed under a stereo microscope. A total of 753 microplastic particles were found in the gastrointestinal tract of 74 individuals (prevalence 99%), and there was a statistically significant difference in the abundance of microplastics found in the gastrointestinal tract of pelagic and demersal fish. The average number of microplastic particles found in the gastrointestinal tract of pelagic fish (12,24 ± 2,43) is higher than in demersal fish (7,38 ± 3,48). The dominant color and shape of microplastic found in the gastrointestinal tract of the fish were black and fiber, respectively. At the same time, the dominant microplastic size found in the gastrointestinal tract of demersal fish was bigger (1,001-5,000 µm, 39,4%) compared to pelagic fish (150-500 µm, 47%). The Fourier Transform Spectroscopy (FTIR) analysis result shows that microplastics of the same polymer type can be found in the gastrointestinal tract of both pelagic and demersal fish. ","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88281090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The demand for Tra fish (Pangasius hypophthalmus) fillets is increasing every year which also increases the number of fish meat byproducts. Approximately 10% of P. hypophthalmus meat is discarded after the production of fish fillets. This study aimed to develop fish balls from the fishmeat byproduct of P. hypophthalmus fillet then investigated the effect of cooking methods and preservation time on the alteration of its texture, brightness, and total bacterial count (TBC). The raw material, minced byproduct and fish balls were analyzed for pH, protein, fat, and moisture contents. The protein content in Tra fish reached 7.35% and increased to 37.14% after the completion of the processing stages for the finished product. Blanching for 4 minutes at 90°C and steaming for 4 minutes at 100°C resulted in good texture and brightness of fish balls. However, a more effective reduction in total bacterial count was observed during the blanching process. Fish balls were preserved by freezing at -40 °C for 42 days and still maintained stable brightness. However, TBC increased significantly after 7 days, and conversely for the texture of fish balls. A finding on the stability of texture and TBC of fish balls when continued preservation from 7 to 42 days. The utilization and use of by-products from the fish fillet processing industry contribute to improving the economic value of the aquaculture industry.
{"title":"Effect of Cooking and Preservation Time on Fish Balls Quality Produced from Pangasius Hypophthalmus Meat By product","authors":"Ngoc Duc Vu, T. T. Tran, V. Nguyen","doi":"10.15578/squalen.739","DOIUrl":"https://doi.org/10.15578/squalen.739","url":null,"abstract":"The demand for Tra fish (Pangasius hypophthalmus) fillets is increasing every year which also increases the number of fish meat byproducts. Approximately 10% of P. hypophthalmus meat is discarded after the production of fish fillets. This study aimed to develop fish balls from the fishmeat byproduct of P. hypophthalmus fillet then investigated the effect of cooking methods and preservation time on the alteration of its texture, brightness, and total bacterial count (TBC). The raw material, minced byproduct and fish balls were analyzed for pH, protein, fat, and moisture contents. The protein content in Tra fish reached 7.35% and increased to 37.14% after the completion of the processing stages for the finished product. Blanching for 4 minutes at 90°C and steaming for 4 minutes at 100°C resulted in good texture and brightness of fish balls. However, a more effective reduction in total bacterial count was observed during the blanching process. Fish balls were preserved by freezing at -40 °C for 42 days and still maintained stable brightness. However, TBC increased significantly after 7 days, and conversely for the texture of fish balls. A finding on the stability of texture and TBC of fish balls when continued preservation from 7 to 42 days. The utilization and use of by-products from the fish fillet processing industry contribute to improving the economic value of the aquaculture industry.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74386081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as a raw material for various natural products, food, and pharmaceutical industries. The main challenge regarding its production, escpecially in Tarakan is the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information and the cultivation performance of the selected seeds by identifying genetic sources based on molecular markers. DNA sequencing analysis was carried out using the molecular marker cox2-3 spacer on a collection comprising 16 cultivars from all over the country (Indonesia). The four haplotypes representing K. alvarezii to be cultivated in the city were produced by phylogenetic clustering and further molecular analysis using cox2-3 intergenic spacer mitochondrial DNA marker. These include the Kupang varieties and Natuna, also referred to as Lampung seedlings, which is a tissue culture plantlet used for development at SEAMEO-BIOTROP Bogor and propagated by the Lampung Marine Aquaculture Center (BBPBL), Tarakan. These varieties were genetically distances (pairwise comparisons based on mean Kimura 2-parameter model) ranging from 0.00-0.23 unit. The cultivation was carried out for 30 days in the waters of Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan, for 30 days. The result showed that the Natuna* as Lampung cultivar has superior performance compared to local cultivars (Takalar as Mamburungan Tanjung Batu, Tarakan, and Kupang). Furthermore, the genetic analysis with cox2-3 spacer markers had the potential as an effective seed selection tool for tracing genetic information in the development of superior seedlings in aquaculture activities.
Kappaphycus alvarezii海藻是一种重要的水产养殖商品，具有很高的经济价值，已被用作各种天然产品、食品和制药工业的原料。生产方面的主要挑战，特别是在塔拉干，是优质种子的供应。因此，本研究旨在通过基于分子标记的遗传来源鉴定，获得所选种子的系统发育信息和培养性能。利用分子标记cox2-3 spacer对来自全国(印度尼西亚)的16个品种进行了DNA测序分析。通过系统发育聚类和cox2-3基因间间隔线粒体DNA标记的分子分析，获得了代表我市拟栽培的4个单倍型。这些品种包括Kupang品种和Natuna，也被称为楠榜幼苗，这是一种组织培养苗，用于SEAMEO-BIOTROP茂物的发育，并由塔拉干的楠榜海洋水产养殖中心(BBPBL)繁殖。这些品种的遗传距离(基于平均Kimura 2参数模型的两两比较)在0.00-0.23单位之间。在丹戎巴图(Tj.)水域进行了30天的培养。在北加里曼丹的塔拉干(Tarakan)的Mamburungan逗留30天。结果表明，纳土纳*作为楠榜品种与当地品种(Takalar作为Mamburungan, Tanjung Batu, Tarakan和Kupang)相比具有优越的性能。此外，利用cox2-3间隔标记进行遗传分析，可作为一种有效的种子选择工具，在水产养殖活动中追踪优良苗的遗传信息。
{"title":"Molecular Assessment of Kappaphycus alvarezii Cultivated in Tarakan based on cox2-3 Spacer","authors":"Dinar Tri Soelistyowati","doi":"10.15578/736","DOIUrl":"https://doi.org/10.15578/736","url":null,"abstract":"The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as a raw material for various natural products, food, and pharmaceutical industries. The main challenge regarding its production, escpecially in Tarakan is the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information and the cultivation performance of the selected seeds by identifying genetic sources based on molecular markers. DNA sequencing analysis was carried out using the molecular marker cox2-3 spacer on a collection comprising 16 cultivars from all over the country (Indonesia). The four haplotypes representing K. alvarezii to be cultivated in the city were produced by phylogenetic clustering and further molecular analysis using cox2-3 intergenic spacer mitochondrial DNA marker. These include the Kupang varieties and Natuna, also referred to as Lampung seedlings, which is a tissue culture plantlet used for development at SEAMEO-BIOTROP Bogor and propagated by the Lampung Marine Aquaculture Center (BBPBL), Tarakan. These varieties were genetically distances (pairwise comparisons based on mean Kimura 2-parameter model) ranging from 0.00-0.23 unit. The cultivation was carried out for 30 days in the waters of Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan, for 30 days. The result showed that the Natuna* as Lampung cultivar has superior performance compared to local cultivars (Takalar as Mamburungan Tanjung Batu, Tarakan, and Kupang). Furthermore, the genetic analysis with cox2-3 spacer markers had the potential as an effective seed selection tool for tracing genetic information in the development of superior seedlings in aquaculture activities.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135394942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microplastic (MP) pollution is an emerging environmental problem that threatens food security, food safety, and human health since it has been reported to be found in commercial fish consumed by humans. Bitung, North Sulawesi, is one of the biggest contributors to capture fishery production in Indonesia. However, there is no data on microplastic pollution in commercial marine fish from Bitung. Therefore, this research aimed to investigate the presence and identify the visual characteristics (color, shape, size) and the polymer type of microplastics found in the gastrointestinal tract of commercial marine fish from Bitung, North Sulawesi. The gastrointestinal tract was extracted using KOH 10%, and the microplastic was observed under a stereo microscope. A total of 753 microplastic particles were found in the gastrointestinal tract of 74 individuals (prevalence 99%), and there was a statistically significant difference in the abundance of microplastics found in the gastrointestinal tract of pelagic and demersal fish. The average number of microplastic particles found in the gastrointestinal tract of pelagic fish (12,24 ± 2,43) is higher than in demersal fish (7,38 ± 3,48). The dominant color and shape of microplastic found in the gastrointestinal tract of the fish were black and fiber, respectively. At the same time, the dominant microplastic size found in the gastrointestinal tract of demersal fish was bigger (1,001-5,000 µm, 39,4%) compared to pelagic fish (150-500 µm, 47%). The Fourier Transform Spectroscopy (FTIR) analysis result shows that microplastics of the same polymer type can be found in the gastrointestinal tract of both pelagic and demersal fish.
{"title":"Abundance and Characteristics of Microplastics Found in The Gastrointestinal Tract of Commercial Marine Fish from Bitung, North Sulawesi – Indonesia","authors":"Aunurohim Aunurohim","doi":"10.15578/719","DOIUrl":"https://doi.org/10.15578/719","url":null,"abstract":"Microplastic (MP) pollution is an emerging environmental problem that threatens food security, food safety, and human health since it has been reported to be found in commercial fish consumed by humans. Bitung, North Sulawesi, is one of the biggest contributors to capture fishery production in Indonesia. However, there is no data on microplastic pollution in commercial marine fish from Bitung. Therefore, this research aimed to investigate the presence and identify the visual characteristics (color, shape, size) and the polymer type of microplastics found in the gastrointestinal tract of commercial marine fish from Bitung, North Sulawesi. The gastrointestinal tract was extracted using KOH 10%, and the microplastic was observed under a stereo microscope. A total of 753 microplastic particles were found in the gastrointestinal tract of 74 individuals (prevalence 99%), and there was a statistically significant difference in the abundance of microplastics found in the gastrointestinal tract of pelagic and demersal fish. The average number of microplastic particles found in the gastrointestinal tract of pelagic fish (12,24 ± 2,43) is higher than in demersal fish (7,38 ± 3,48). The dominant color and shape of microplastic found in the gastrointestinal tract of the fish were black and fiber, respectively. At the same time, the dominant microplastic size found in the gastrointestinal tract of demersal fish was bigger (1,001-5,000 µm, 39,4%) compared to pelagic fish (150-500 µm, 47%). The Fourier Transform Spectroscopy (FTIR) analysis result shows that microplastics of the same polymer type can be found in the gastrointestinal tract of both pelagic and demersal fish.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135394946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sibero, Adella Maulina Savitri, E. H. Frederick, Sri Sedjati
Gracilaria verrucosa is a red seaweed that has been widely utilized in the food andpharmaceutical industries due to its biological properties. The utilization of biologicalagents in obtaining certain bioactive compounds would confront unavoidable issues,particularly its bioactive sustainability. Hence, microbial fermentation has been reported as a practical approach to maintaining bioactive production and boosting its properties. Our study aimed to evaluate the potential of marine yeast Aureobasidium melanogenum MTGK.31 as a fermenting agent for G. verrucosa and characterize the seaweed metabolite profile and antioxidant activity after fermentation. The seaweed was fermented using A. melanogenum MTGK.31 in a medium consisting of yeast extract, peptone, and glucose. The fermentation was done for 24, 48, and 72 hours. Total plate count and pH were measured after each fermentation period. The primary and secondary metabolites of G. verrucosa in each fermentation were observed. Antioxidant assay using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method was conducted, followed by total phenolic content using the Folin-Ciocalteu method. It was highlighted that yeast colony increased during the fermentation, while the pH level was decreasing. We found that the fermentation not only boosted some elements in primary metabolites, but also increased G. verrucosa bioactive groups. After 72 hours of fermentation, the G. verrucosa percent radical scavenging activity (%RSA) increased more than two times compared to the fresh G. verrucosa with a %RSA value of 16.09±6.57. Nevertheless, the highest total phenolic content of 5.62±0.00028 mg GAE/g extract was shown by G. verrucosa after 48 hours of fermentation.
{"title":"Metabolites Alteration and Antioxidant Activity of Gracilaria verrucosa After Fermentation Using Aureobasidium melanogenum MTGK.31","authors":"M. Sibero, Adella Maulina Savitri, E. H. Frederick, Sri Sedjati","doi":"10.15578/squalen.727","DOIUrl":"https://doi.org/10.15578/squalen.727","url":null,"abstract":"Gracilaria verrucosa is a red seaweed that has been widely utilized in the food andpharmaceutical industries due to its biological properties. The utilization of biologicalagents in obtaining certain bioactive compounds would confront unavoidable issues,particularly its bioactive sustainability. Hence, microbial fermentation has been reported as a practical approach to maintaining bioactive production and boosting its properties. Our study aimed to evaluate the potential of marine yeast Aureobasidium melanogenum MTGK.31 as a fermenting agent for G. verrucosa and characterize the seaweed metabolite profile and antioxidant activity after fermentation. The seaweed was fermented using A. melanogenum MTGK.31 in a medium consisting of yeast extract, peptone, and glucose. The fermentation was done for 24, 48, and 72 hours. Total plate count and pH were measured after each fermentation period. The primary and secondary metabolites of G. verrucosa in each fermentation were observed. Antioxidant assay using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method was conducted, followed by total phenolic content using the Folin-Ciocalteu method. It was highlighted that yeast colony increased during the fermentation, while the pH level was decreasing. We found that the fermentation not only boosted some elements in primary metabolites, but also increased G. verrucosa bioactive groups. After 72 hours of fermentation, the G. verrucosa percent radical scavenging activity (%RSA) increased more than two times compared to the fresh G. verrucosa with a %RSA value of 16.09±6.57. Nevertheless, the highest total phenolic content of 5.62±0.00028 mg GAE/g extract was shown by G. verrucosa after 48 hours of fermentation.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"122 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76688870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The demand for Tra fish (Pangasius hypophthalmus) fillets is increasing every year which also increases the number of fish meat byproducts. Approximately 10% of P. hypophthalmus meat is discarded after the production of fish fillets. This study aimed to develop fish balls from the fishmeat byproduct of P. hypophthalmus fillet then investigated the effect of cooking methods and preservation time on the alteration of its texture, brightness, and total bacterial count (TBC). The raw material, minced byproduct and fish balls were analyzed for pH, protein, fat, and moisture contents. The protein content in Tra fish reached 7.35% and increased to 37.14% after the completion of the processing stages for the finished product. Blanching for 4 minutes at 90°C and steaming for 4 minutes at 100°C resulted in good texture and brightness of fish balls. However, a more effective reduction in total bacterial count was observed during the blanching process. Fish balls were preserved by freezing at -40 °C for 42 days and still maintained stable brightness. However, TBC increased significantly after 7 days, and conversely for the texture of fish balls. A finding on the stability of texture and TBC of fish balls when continued preservation from 7 to 42 days. The utilization and use of by-products from the fish fillet processing industry contribute to improving the economic value of the aquaculture industry.
{"title":"Effect of Cooking and Preservation Time on Fish Balls Quality Produced from Pangasius Hypophthalmus Meat By product","authors":"Ngoc Duc Vu, Thanh Truc Tran, Van Muoi Nguyen","doi":"10.15578/739","DOIUrl":"https://doi.org/10.15578/739","url":null,"abstract":"The demand for Tra fish (Pangasius hypophthalmus) fillets is increasing every year which also increases the number of fish meat byproducts. Approximately 10% of P. hypophthalmus meat is discarded after the production of fish fillets. This study aimed to develop fish balls from the fishmeat byproduct of P. hypophthalmus fillet then investigated the effect of cooking methods and preservation time on the alteration of its texture, brightness, and total bacterial count (TBC). The raw material, minced byproduct and fish balls were analyzed for pH, protein, fat, and moisture contents. The protein content in Tra fish reached 7.35% and increased to 37.14% after the completion of the processing stages for the finished product. Blanching for 4 minutes at 90°C and steaming for 4 minutes at 100°C resulted in good texture and brightness of fish balls. However, a more effective reduction in total bacterial count was observed during the blanching process. Fish balls were preserved by freezing at -40 °C for 42 days and still maintained stable brightness. However, TBC increased significantly after 7 days, and conversely for the texture of fish balls. A finding on the stability of texture and TBC of fish balls when continued preservation from 7 to 42 days. The utilization and use of by-products from the fish fillet processing industry contribute to improving the economic value of the aquaculture industry.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135394943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Healthy seaweed salt is low sodium salt from seaweed that offers health benefits for hypertension patients. Indonesian seaweed has the potential to produce healthy seaweed salt. Research to date still focuses on green and brownseaweed but there is still no report for red seaweed. Actinotrichia fragilis isone of red seaweed species that has been discovered in Indonesia’s seawaterand has not yet been utilized. Thus, this study aimed to determine the chemicalcomposition and antioxidant activity of A. fragilis flour and the optimum ratiofor producing seaweed salt with a high yield, optimum %NaCl, Na/K ratio, andantioxidant activity. Seaweed salt production treatment was the ratio of seaweed flour and distilled water 1:3, 1:5, and 1:10 (w/v), extracted at 40°C for 10minutes. The mixture was filtered, then dried at 60°C for 30 hours. Data analysiswas performed by analysis of variance. The raw material for dried A. fragilisseaweed has a high ash and low-lipid content. Then the ethanol extract had atotal phenolic content value of 84.34 mg GAE/g and an antioxidant activityvalue of 98.22 mg/L. Furthermore, the antioxidant capacity of the ethanol extract was 60.15 nmol ascorbic acid/g and 552.21 n mol Fe2+/g. The best treatmentfor producing A. fragilis salt is 1:10 with yield of 12.76±0.13%, %NaCl47.22±1.38%, Na/K ratio 3.32±0.18, IC50 with DPPH and ABTS method 113 mg/Land 87.27 mg/L, total antioxidant capacity 38.21 n g/mL ascorbic acid/g, and304.32 n mol Fe2+/g. Furthermore, A. fragilis can be used for the production ofhealthy seaweed salt.
健康海藻盐是从海藻中提取的低钠盐，对高血压患者的健康有益。印度尼西亚的海藻具有生产健康海藻盐的潜力。迄今为止的研究仍然集中在绿海藻和褐海藻上，但仍然没有关于红海藻的报道。脆弱放线菌是在印度尼西亚海水中发现的一种红海藻，尚未被利用。因此，本研究旨在确定脆皮藻粉的化学成分和抗氧化活性，以及生产高产海藻盐的最佳配比、最佳NaCl %、Na/K比和抗氧化活性。海藻盐生产处理为海藻粉与蒸馏水的比例分别为1:3、1:5和1:10 (w/v)，在40℃下提取10min。将混合物过滤，然后在60°C下干燥30小时。数据分析采用方差分析。干燥软藻原料灰分高，脂质含量低。乙醇提取物的总酚含量为84.34 mg GAE/g，抗氧化活性为98.22 mg/L。乙醇提取物的抗氧化能力分别为60.15 nmol抗坏血酸/g和552.21 nmol Fe2+/g。最佳产盐条件为1:10，产率为12.76±0.13%，% nacl为47.22±1.38%，Na/K比为3.32±0.18,DPPH和ABTS法IC50为113 mg/Land 87.27 mg/L，总抗氧化能力为38.21 ng /mL抗坏血酸/g, Fe2+为304.32 n mol /g。此外，脆弱芽孢杆菌可用于生产健康的海藻盐。
{"title":"Study of Actinotrichia fragilis Indonesian Red Seaweed as Raw Material for Healthy Salt","authors":"Nurjanah Nurjanah, Agoes Mardiono Jacoeb, Asadatun Abdullah, Joko Ahadi Priyanto, Naufal Muharam Nurdin, Anggrei Viona Seulalae","doi":"10.15578/753","DOIUrl":"https://doi.org/10.15578/753","url":null,"abstract":"Healthy seaweed salt is low sodium salt from seaweed that offers health benefits for hypertension patients. Indonesian seaweed has the potential to produce healthy seaweed salt. Research to date still focuses on green and brownseaweed but there is still no report for red seaweed. Actinotrichia fragilis isone of red seaweed species that has been discovered in Indonesia’s seawaterand has not yet been utilized. Thus, this study aimed to determine the chemicalcomposition and antioxidant activity of A. fragilis flour and the optimum ratiofor producing seaweed salt with a high yield, optimum %NaCl, Na/K ratio, andantioxidant activity. Seaweed salt production treatment was the ratio of seaweed flour and distilled water 1:3, 1:5, and 1:10 (w/v), extracted at 40°C for 10minutes. The mixture was filtered, then dried at 60°C for 30 hours. Data analysiswas performed by analysis of variance. The raw material for dried A. fragilisseaweed has a high ash and low-lipid content. Then the ethanol extract had atotal phenolic content value of 84.34 mg GAE/g and an antioxidant activityvalue of 98.22 mg/L. Furthermore, the antioxidant capacity of the ethanol extract was 60.15 nmol ascorbic acid/g and 552.21 n mol Fe2+/g. The best treatmentfor producing A. fragilis salt is 1:10 with yield of 12.76±0.13%, %NaCl47.22±1.38%, Na/K ratio 3.32±0.18, IC50 with DPPH and ABTS method 113 mg/Land 87.27 mg/L, total antioxidant capacity 38.21 n g/mL ascorbic acid/g, and304.32 n mol Fe2+/g. Furthermore, A. fragilis can be used for the production ofhealthy seaweed salt.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":"155 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135394962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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