Plant, Cell & Environment最新文献

Water storage capacity and capacitance in trees regulate hydration levels, providing water reserves during drought. However, the effects of varying traits, tissue fractions and of different water pools on the allometry of branch-/sample-level properties have not been systematically investigated. We analyse the relationships between branch size and branch capacity and capacitance with respect to wood density, xylem vulnerability to embolism, and tissue fractions. The analysis was performed using data from four tree species sampled from 12 to 15 sites across Europe. We show that of the three phases of the water release curve, the second phase (dominated by elasticity) was significantly influenced by leaf and bark proportions, the sapwood/heartwood ratio and xylem vulnerability to embolism for capacity and/or capacitance. However, the first (dominated by capillarity) and the third phase (characterised by embolism) were not influenced by the morpho-physiological properties measured. Our results indicate that branch capacity and capacitance are allometrically related (slope < 1) to branch dry mass, leaf area and total water content, indicating that normalising by these size measures does not completely remove size-dependency. We conclude that the only means of obtaining size-independent water storage traits directly applicable in comparative and modelling studies is by normalising by water quantity per phase.

The generation of spectral libraries using hyperspectral data allows for the capture of detailed spectral signatures, uncovering subtle variations in plant physiology, biochemistry, and growth stages, marking a significant advancement over traditional land cover classification methods. These spectral libraries enable improved forest classification accuracy and more precise differentiation of plant species and plant functional types (PFTs), thereby establishing hyperspectral sensing as a critical tool for PFT classification. This study aims to advance the classification and monitoring of PFTs in Shoolpaneshwar wildlife sanctuary, Gujarat, India using Airborne Visible/Infrared Imaging Spectrometer-Next Generation (AVIRIS-NG) and machine learning techniques. A comprehensive spectral library was developed, encompassing data from 130 plant species, with a focus on their spectral features to support precise PFT classification. The spectral data were collected using AVIRIS-NG hyperspectral imaging and ASD Handheld Spectroradiometer, capturing a wide range of wavelengths (400-1600 nm) to encompass the key physiological and biochemical traits of the plants. Plant species were grouped into five distinct PFTs using Fuzzy C-means clustering. Key spectral features, including band reflectance, vegetation indices, and derivative/continuum properties, were identified through a combination of ISODATA clustering and Jeffries-Matusita (JM) distance analysis, enabling effective feature selection for classification. To assess the utility of the spectral library, three advanced machine learning classifiers-Parzen Window (PW), Gradient Boosted Machine (GBM), and Stochastic Gradient Descent (SGD)-were rigorously evaluated. The GBM classifier achieved the highest accuracy, with an overall accuracy (OAA) of 0.94 and a Kappa coefficient of 0.93 across five PFTs.

Nano-selenium fertilizers can promote plant growth and nitrogen availability. However, little information is available on the effects of nano-selenium on tea leaf quality, soil nutrient availability and associated microbe-driven mechanisms. This study examined the effects of nano-selenium on the tea leaf quality and soil nitrogen cycling in 20-year-old tea plantations when the leaves were sprayed with ammonium or nitrate. Leaf selenium and amino acid contents increased ninefold and 9%, respectively, with nano-selenium in "Zhongcha108" and "Longjing43." Rhizosphere bacterial and fungal community compositions were more sensitive to selenium and nitrogen applications in "Longjing43" than in "Zhongcha108." "Zhongcha108" enriched more taxa related to microbial growth, while more taxa related to cellular maintenance and nutrient acquisition enriched in "Longjing43." Nano-selenium application decreased the copy number of AOA and AOB genes, and nosZ and nirK genes by 59%, 53%, 37% and 46% under ammonium, and by 77%, 43%, 38% and 65%, respectively, under nitrate spraying, in "Longjing43." However, the expression of these genes increased by nano-selenium in "Zhongcha108" with ammonium spraying. It is concluded that a nano-selenium application increases tea leaf quality, and this effect on nitrogen cycling and ecological functioning largely depends on the tea cultivar-specific bacterial and fungal composition and function.

Transgenerational plasticity in plants is an increasingly recognized phenomenon, yet it is mostly unclear whether transgenerational plasticity is relevant to both the fitness of the plant and its interacting species. Using monoclonal strains of the giant duckweed (Spirodela polyrhiza) and its native herbivore, the waterlily aphid (Rhopalosiphum nymphaeae), we assessed whether pre-treating plants with copper excess, both indoors and outdoors, induces transgenerational plasticity in plant defences that alter plant and herbivore fitness. Outdoors, copper pre-treatment tended to increase plant growth rates under recurring copper excess. Indoors, copper pre-treatment either increased or decreased plant growth rates under recurring conditions, depending on the plant genotype. Copper pre-treatment induced anthocyanins that protected plants against copper toxicity, and these elevated levels were transgenerationally retained. Copper pre-treatment also transgenerationally increased the levels of 12-oxo-phytodienoic acid (OPDA), a jasmonate precursor. Nevertheless, aphids grew up to 50% better when the plants were pre-treated with copper. The increased aphid growth was likely caused by transgenerationally elevated OPDA levels, as aphids grew better when jasmonates were externally applied to plants. Taken together, this study shows that transgenerational plasticity is relevant to both plant and herbivore fitness, which highlights the role of transgenerational plasticity in plant evolution and species interactions.

Beyond structural support, plant root systems play crucial roles in the absorption of water and nutrients, fertiliser efficiency and crop yield. However, the molecular mechanism regulating root architecture in rice remains largely unknown. In this study, a short-root rice mutant was identified and named Oscyp22. Oscyp22 showed impairment in the growth of primary, adventitious and lateral roots. Histochemical and fluorescent staining analyses revealed reduced cell elongation and division activity in the root of Oscyp22. Further analysis showed that Oscyp22 displayed an impaired response to auxin treatment, indicating a disruption in the auxin signal transduction. Transcriptome analysis and auxin content measurement suggested that OsCYP22 might be involved in auxin synthesis and transport. Protein assays demonstrated that OsCYP22 could interact with OsCSN5 and induce its rapid degradation. Notably, Oscsn5 mutants also showed short root phenotypes and deficiencies in auxin response. These findings suggest that OsCYP22 plays a role in rice root growth potentially through auxin signalling and OsCSN5 stability.

Calcium (Ca²⁺)-dependent signalling plays a well-characterised role in the perception and response mechanisms to environmental stimuli in plant cells. In the context of a constantly changing environment, it is fundamental to understand how crop yield and microalgal biomass productivity are affected by external factors. Ca²⁺ signalling is known to be important in different physiological processes in microalgae but many of these signal transduction pathways still need to be characterised. Here, compartment-specific Ca²⁺ dynamics were monitored in Chlamydomonas reinhardtii cells in response to environmental stressors, such as nutrient availability, osmotic stress, temperature fluctuations and carbon sensing. An in vivo single-cell imaging approach was adopted to directly visualise changes of Ca2+ concentrations at the level of specific subcellular compartments, using C. reinhardtii lines expressing a genetically encoded ratiometric Ca²⁺ indicator. Hyper-osmotic shock caused cytosolic and chloroplast Ca²⁺ elevations, whereas high temperature and inorganic carbon availability primarily induced Ca²⁺ transients in the chloroplast. In contrast, hypo-osmotic stress only induced Ca²⁺ elevations in the cytosol. The results herein reported show that in Chlamydomonas cells compartment-specific Ca²⁺ transients are closely related to specific external environmental stimuli, providing useful guidance for studying signal transduction mechanisms exploited by microalgae to respond to specific natural conditions.

Vigna marina (Barm.) Merr. is adapted to tropical marine beaches and has an outstanding tolerance to salt stress. Given there are growing demands for cultivating crops in saline soil or with saline water, it is important to understand how halophytic species are adapted to the saline environments. Here we revealed by positron-emitting tracer imaging system (PETIS) that V. marina actively excretes sodium from the root during the light period but not the dark period. The following whole genome sequencing accompanied with forward genetic study identified a QTL region harbouring SOS1, encoding plasma membrane Na⁺/H⁺ antiporter, which was associated with not only salt tolerance but also the ability of sodium excretion. We also found the QTL region contained a large structural rearrangement that suppressed recombination across ~14 Mbp, fixing multiple gene loci potentially involved in salt tolerance. RNA-seq and promoter analyses revealed SOS1 in V. marina was highly expressed even without salt stress and its promoter shared common cis-regulatory motifs with those exhibiting similar expression profiles. Interestingly, the cis-regulatory motifs seemed installed by a transposable element (TE) insertion. Though not identified by genetic analysis, the transcriptome data also revealed SOS2 transcription was under diurnal regulation, explaining the pattern of sodium excretion together with upregulated expression of SOS1. Altogether, the study elucidated one aspect of the strategy adopted by V. marina to adapt to marine beach, which is highly saline and transpiring.

Plant internal phosphorus (P) recycling is a complex process, which is vital for improving plant P use efficiency. However, the mechanisms underlying phosphate (Pi) release from internal organic-P form remains to be deciphered in crops. Here, we functionally characterised a Pi-starvation responsive purple acid phosphatase (PAP), GmPAP23 in soybean (Glycine max). GmPAP23 could hydrolyse a series of Pi-containing compounds in vitro, such as trehalose-6-phosphate and glucose-l-phosphate. Moreover, GmPAP23 overexpression led to less P distribution in soybean source organs, including mature leaves and pod shells, but more P distribution in seeds under P sufficient conditions, although no effect was observed for transgenic soybean lines with its suppression. Metabolomic analysis found that a group of P-containing metabolites exhibited differential accumulations in mature leaves between wild type (WT) and GmPAP23 overexpression lines, such as glucose-l-phosphate and trehalose-6-phosphate. Moreover, a MYB transcription factor, GmPHR14 was subsequently found to activate the transcription of GmPAP23 via directly binding to its promoter. Collectively, these findings could highlight that the GmPHR14-GmPAP23 pathway, which controls internal P recycling in soybean, and thus affect yield.

Chlamydomonas reinhardtii, a prominent chassis in synthetic biology, faces limitations in regulating the expression of exogenous genes. A destabilization domain (DD)/Shield-1 system, originally derived from mammals, offers a ligand-dependent control of stability, making it a valuable tool. This system utilises the destabilization domain to induce rapid degradation of target protein unless stabilised by Shield-1, a synthetic ligand. Upon the addition of Shield-1,the degradation is halted, leading to the accumulation and stabilisation of the target protein. This system has demonstrated successful regulation of foreign protein expression in mammals, parasites, and plants. In this study, the DD/Shield-1 system was harnessed to regulate the expression of the paromomycin resistance gene and luciferase encoding gene in Chlamydomonas, revealing its capability for rapid, stable, and reversible protein expression regulation in microalgae, serving as a molecular switch. Furthermore, this regulation exhibits reagent dependency, enhancing its applicability in practical production. A strain with induced expression of the gene-editing protein, LbCas12a, was successfully constructed and then tested for gene editing. The findings not only enrich the toolkit for Chlamydomonas molecular studies but offer a promising technique for regulating the expression and validating the functionality of exogenous proteins in microalgae.

A key feature of stress responses [closely relative to the phytohormone abscisic acid (ABA)] and associated acclimation in plants is the dynamic adjustments and related optimisation of carbohydrate content between sink and source organs. The production of stomata, which consist of a pore between two adjacent guard cells, are central to plant adaptation to changing environment conditions. In this context, ABA is a core modulator of environmentally determined stomatal development. It is well established that ABA reduces stomatal aperture but in addition, this hormone also enhances stomatal production. For example, when grown at very high CO₂ levels, Arabidopsis lines with defects in ABA biosynthesis have reduced stomatal production. By contrast, under standard conditions, these ABA biosynthetic mutants show increased stomatal production. However, the molecular mechanism(s) underpinning this regulation remain largely unknown. In this review, we summarise the current knowledge concerning sucrose transport and ABA signalling relating to stomatal production. Further, we provide fresh insights into the possible function of photosynthetic product induced-ABA as a balancer of carbohydrate accumulation between sink and source organs, to control stomatal production and thereby to adjust stress responses and acclimation in plants, by controlling sucrose transport systems.