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Comparison and Development of A Rapid Extraction Methods of DNA from Ancient Human Skeletal Remains of Turkey 土耳其古人类遗骸DNA快速提取方法的比较与发展
Pub Date : 2008-12-31 DOI: 10.5580/1fa8
H. Vural, Ahmet Adil Tırpan
The use of genetic technology in forensic science is applied primarily to distinguish between individials who may be the source of biological material associated with archeological remains. DNA sequences from ancient fossils have great potential for studies of phylogeny, biogeography and molecular evolution. DNA from fossils also facilitates the rigorous testing and calibration of mutation rates among related taxa, sex test and molecular divergence time. In this study, a rapid and quantitative aDNA extaction methods from human skeletal remains was developed for application of forensic science and archeometry. For that reason, DNA was extracted from ancient human bones from Mugla in Turkey. Extraction of DNA was carried out using the laboratuary handling and cleaning protocol. After cleaning of bone, small piece of ancient bones were ground to powder with a mixer mill. Aliquots of the powder were subjected to a calfication method and extracted with 0.5 M EDTA (pH 8.3) for 48 hours at 56 ∞ C. After addition of proteinase K, solution of bone was incubated at 37 ∞ C. Genomic DNA from supernatant was extracted automatically by using EZ1 Automatic Nucleic Acid Isolation System (Qiagen, Germany) with investigator kit (Qiagen, Ilden, Germany) and different DNA extraction methods which are modified by researcher from ancient bones. EZ1 Nucleic acid isolation method; This tehnique is quite useful for high yield and quality of aDNA isolation from human skeletal remains. In this methods, no further purification was needed for molecular analysis. Amount and purity of extracted DNA from ancient bones were measured by Spectrophotometer. In addition to spectrophotometric measurement, extracted DNA was applied to 1 % agarose gel, stained and imaged under ultraviolet (UV) irradiation. As a result, 50 ng pure DNA was extracted from ancient bones, approximately 1.8. This protocol proved to be advantageous because of its simplicity, quickness and affordable reagents, besides the high molecular weight DNA and purity achieved in a variety of fosil bone tissues from the total set obtained from Mugla in Turkey.
基因技术在法医科学中的应用主要是为了区分可能是与考古遗迹有关的生物材料来源的个人。古化石DNA序列在系统发育、生物地理学和分子进化研究中具有巨大的潜力。来自化石的DNA还有助于相关分类群之间的突变率、性别测试和分子分化时间的严格测试和校准。本研究开发了一种快速、定量提取人类骨骼遗骸dna的方法,用于法医科学和考古测量。因此,从土耳其穆拉的古人类骨骼中提取了DNA。DNA提取采用实验室处理和清洗方案进行。骨头清洗后,小块古代骨头用搅拌机磨成粉末。等分粉末经鉴定,用0.5 M EDTA (pH 8.3)在56∞c下提取48小时,加入蛋白酶K后,骨液在37∞c下孵育,使用EZ1自动核酸分离系统(Qiagen, Germany)和研究者试剂盒(Qiagen, Ilden, Germany)和研究者改进的不同DNA提取方法自动提取上清中的基因组DNA。EZ1核酸分离法;该技术对高产量、高质量地从人骨遗骸中分离aDNA具有重要意义。在这种方法中,不需要进一步纯化进行分子分析。用分光光度计测定古骨DNA的提取量和纯度。除分光光度法测定外,提取的DNA应用于1%琼脂糖凝胶,在紫外线照射下染色和成像。结果,从古代骨骼中提取了50 ng的纯DNA,大约1.8 ng。该方案被证明是有利的,因为它的简单,快速和负担得起的试剂,除了高分子量的DNA和纯度的各种化石骨组织从土耳其Mugla获得的集合。
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引用次数: 3
Anogenital distance in human male and female newborns: A look at a cross section of a Nigerian population. 人类男性和女性新生儿的肛门生殖器距离:尼日利亚人口的横截面。
Pub Date : 2008-12-31 DOI: 10.5580/a28
C. Orish, B. Didia
A cross-sectional study was conducted among the newborn children at the University of Port Harcourt Teaching Hospital and Braithwaite Memorial Hospital all in Port Harcourt, Rivers State, Nigeria. The study included 139 newborn infants (80 males and 59 females), born at term, with no congenital defects. Birth weight, length, and anogenital distance AGD, were made with tape by two independent observers. Distance was measured from the center of the anus to the posterior convergence of the fourchette in females; and from the center of the anus to the junction of the smooth perineal skin with the rugated skin of the scrotum in males. Student‘s T-test and analysis of variance were used to compare male-female measurements using Graph Pad Prism 3.0. The AGD in males and females were 3.02 ± 0.14 and 2.58 ± 0.11 cm respectively. There was significant difference between the AGD values in male and female babies in a Nigerian population.
在尼日利亚河流州哈科特港哈科特港大学教学医院和布雷斯韦特纪念医院的新生儿中进行了一项横断面研究。该研究包括139名新生儿(80名男性和59名女性),足月出生,没有先天性缺陷。出生体重、体长和肛门生殖器距离AGD由两名独立观察员用胶带记录。测量雌性肛门中心至肛门后会聚处的距离;从肛门中心到光滑的会阴皮肤和男性阴囊凹凸不平的皮肤的交界处。采用学生t检验和方差分析比较使用graphpad Prism 3.0的男女测量值。男性和女性的AGD分别为3.02±0.14和2.58±0.11 cm。在尼日利亚人群中,男性和女性婴儿的AGD值存在显著差异。
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引用次数: 8
Haptoglobin polymorphism among three populations of Manipur, India: a critical analysis 印度曼尼普尔三个种群的珠蛋白多态性:一个关键的分析
Pub Date : 2008-12-31 DOI: 10.5580/71c
M. Asghar, N. Achoubi, S. Y. Meitei, K. Meitei, B. Murry, M. Sachdeva, K. Saraswathy
Haptogloin is a plasma protein that binds free hemoglobin, thereby inhibiting hemoglobin induced oxidative damage. The distribution of haptoglobin alleles varies according to the geographical location and ethnicity. The present study is carried out to study the frequency distribution of haptoglobin in three endogamous populations of Manipur, India. Serum was separated from blood samples collected from 335 Muslims, 262 Meitei Brahmins and 121 Kabuis. HP*2 is found to be highest among Kabuis (0.924) followed by Muslims (0.918) and Meitei Brahmins (0.836). Muslims are found to deviate from the Hardy-Weinberg equilibrium (p=0.006). Comparison between the populations show a significant difference between Muslims and Meitei Brahmins (p=0.00) and Meitei Brahmins and Kabuis (p=0.005). The frequency of HP*2 is also found to have increased significantly in comparison to earlier reported data, supporting the hypothesis that haptoglobin polymorphism is still in transient form.
粘连蛋白是一种结合游离血红蛋白的血浆蛋白,从而抑制血红蛋白引起的氧化损伤。触珠蛋白等位基因的分布因地理位置和种族而异。本研究是在印度曼尼普尔邦的三个内婚种群中进行的,目的是研究接触珠蛋白的频率分布。从335名穆斯林、262名美泰婆罗门和121名Kabuis人的血液样本中分离血清。HP*2在Kabuis(0.924)中最高,其次是穆斯林(0.918)和Meitei婆罗门(0.836)。穆斯林被发现偏离了Hardy-Weinberg平衡(p=0.006)。人口之间的比较显示,穆斯林和美泰婆罗门(p=0.00)以及美泰婆罗门和Kabuis (p=0.005)之间存在显著差异。与先前报道的数据相比,HP*2的频率也显著增加,支持了接触珠蛋白多态性仍处于短暂形式的假设。
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引用次数: 2
Estimation of Hand Grip Strength and its Association with some Anthropometric traits in Cricketers of Amritsar, Punjab, India 印度旁遮普阿姆利则板球运动员握力及其与人体测量特征的关系
Pub Date : 2008-12-31 DOI: 10.5580/18de
S. Koley, M. Yadav, J. Sandhu
The present study is based on the association of left and right hand grip strength with eight anthropometric traits, viz. height, weight, body mass index, hand length, hand breadth, upper arm length, forearm length and total arm length in 100 male cricketers aged 17 – 21 years (mean age 18.29 ± 2.21 years ) of Amritsar, Punjab, India. A total of 100 controls were also taken for comparisons. The findings of the present study indicate a strong association of right hand grip strength with height (r =0.383), weight (r=0.498), body mass index (r=0.401), hand length (r=0.444), hand breadth (r=0.326) and forearm length (r=0.215). Whereas left hand grip strength was reported to be closely associated with height (r=0.355), weight (r=0.472), body mass index (r=0.374), hand length (r=0.320) and hand breadth (r=0.330).
本研究以印度旁遮普邦阿姆利则地区100名17 - 21岁(平均年龄18.29±2.21岁)男子板球运动员为研究对象,分析了左手和右手握力与身高、体重、体质指数、手长、手宽、上臂长、前臂长和总臂长8项人体测量特征的关系。总共有100个对照进行比较。研究结果表明,右手握力与身高(r= 0.383)、体重(r=0.498)、体质指数(r=0.401)、手长(r=0.444)、手宽(r=0.326)、前臂长(r=0.215)有较强的相关性。而据报道,左手握力与身高(r=0.355)、体重(r=0.472)、体重指数(r=0.374)、手长(r=0.320)和手宽(r=0.330)密切相关。
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引用次数: 21
Enhancing the quality of The Internet Journal of Biological Anthropology 提高《生物人类学网络学报》的质量
Pub Date : 2008-12-31 DOI: 10.5580/1f7a
K. Krishan
I am happy to announce to members of editorial board of The Internet Journal of Biological Anthropology, contributing authors, and to all our readers that in early 2009, The Internet Journal of Biological Anthropology was selected for indexing and inclusion in SCOPUS the largest abstract and citation database of quality peer-reviewed research literature in scientific, medical, Engineering and social sciences fields. According to the letter sent by Scopus, this was in recognition of the high quality and relevance to the scientific community of the journal. Ever since the establishment of the journal in June 20071, we have been interesting in having our journal indexed in Scopus and have made every effort to make the journal an international publication of quality. In the later part of 2008, The Internet Journal of Biological Anthropology was evaluated by board of technical review committee for its quality, originality and importance in the field of Anthropology, and was adjudged to be worthy of inclusion. This will definitely lead to an increase in the number of submissions to the journal and also to an increase in the number of citations of the articles from the journal cited by authors in other publications. We believe that this indexing will serve as means for the further development of the journal receiving the kind of articles that stimulate and gratify the new developments in the field of biological anthropology. I, along with the editorial board of the journal, would like to express sincere gratitude to our publisher Internet Scientific Publications for their support throughout. The availability of the full text on the website to all our readers is an added benefit for the growth of the journal and was helpful in obtaining the results we wanted. As you already know that inclusion in abstract & indexing (A&I) databases increases the visibility and awareness of full-text journals. The A&I databases will drive usage and traffic to the full-text platforms with sophisticated linking technologies, increasing journal brand awareness and subscription sales. Indexing in Scopus and Elsevier Bibliographic databases will enable readers to search and retrieve all articles from past and current issues. While the full content of articles has always been immediately available online to readers worldwide at http://www.ispub.com at no charge, the Scopus will make titles, abstracts and references available to people conducting searches for academics, research and development. Every journal editor attempts to ensure what is best for the journal for its survival and its well being such that not only do the number of readers increase but so that quality of the articles published is also enhanced. It is with this in mind since the last two years23 when the journal came into existence, many attempts have been made to improve the quality of the journal. We, of course, welcome our readers to contribute to this effort by giving in their views. One result of these end
我们希望你喜欢阅读这期的新发现和最新评论。因此,我们期待收到您的论文,并邀请您对《生物人类学互联网杂志》的改进提出建议和意见。请通过电子邮件(gargkk@yahoo.com)向我们发送您的意见、问题、查询和提交前查询。Kewal Krishan,主编
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引用次数: 7
Sexual Dimorphism In Foot Dimensions Among Adult Nigerians 尼日利亚成年人足部尺寸的性别二态性
Pub Date : 2008-12-31 DOI: 10.5580/2e5
I. Bob-Manuel, B. Didia
This study is aim at quantitatively analysing physical difference that exist in foot dimensions in males and in females and within both sexes thus providing normal value for foot length and foot breadth for the design and construction of a comfortable foot support in our population. Sexual dimorphism in foot dimension exists within and across both sexes. These gender differences in foot dimension may not be known until they are quantified, as they appear symmetrical at first sight. The foot length and breadth of 477 (249 males and 28 females) individuals were measured directly using the sliding calliper. Their age ranged between 18yrs and above. The mean values for the right foot length of males and females were 26.92 ± 1.02 and 25.00 ± 1.33 respectively. The mean value for the right foot breadth of males and females were 9.87 ± 0.53 and 9.14 ± 0.58 respectively. The mean values for the left foot length of males and females were 26.92 ± 0.13 and 24.75 ± 0.17 respectively. The mean value for the right foot breadth of males and females were 9.75 ± 0.07and 8.92 ± 0.08 respectively. Males had significantly higher values of foot length and foot breadth than females, p < 0.001. Males have longer and broader feet than females for a given age. Female feet are not simply scaled – down versions of male feet. Males and females feet in our population had higher mean foot length than that of Caucasians, which is an adaptation to tropical climate. These findings are useful to shoe design and selection of shoe sizes and to forensic anthropometry.
本研究旨在定量分析男性和女性以及两性之间存在的足部尺寸生理差异,从而为设计和建造舒适的足部支撑提供足长和足宽的正常值。足部尺寸的两性二态性存在于两性内部和两性之间。这些足部尺寸的性别差异在量化之前可能无法得知,因为它们乍一看似乎是对称的。用滑动卡尺直接测量了477只(雄性249只,雌性28只)的足长和足宽。他们的年龄在18岁以上。男性和女性的右脚长度平均值分别为26.92±1.02和25.00±1.33。男性和女性右脚宽度平均值分别为9.87±0.53和9.14±0.58。男性和女性的左脚长度平均值分别为26.92±0.13和24.75±0.17。男性和女性右脚宽度平均值分别为9.75±0.07和8.92±0.08。男性的脚长和脚宽显著高于女性,p < 0.001。在一定的年龄,雄性的脚比雌性的更长更宽。女性的脚并不是男性脚的简单缩小版。种群中男性和女性的平均脚长均高于高加索人,这是对热带气候的适应。这些发现对鞋的设计和鞋码的选择以及法医人体测量都是有用的。
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引用次数: 25
Impact of gender bias on health and nutrition of the tribal women in relation to dynamics of development in India. 性别偏见对部落妇女健康和营养的影响与印度发展动态的关系。
Pub Date : 2008-12-31 DOI: 10.5580/15ab
R. Balgir
Tribal communities in India are economically and socially backward and mostly live in forests and hilly terrains isolated from the other elite communities. They have their own way of living and different socio-cultural and eco-geographical settings. Lack of proper education and health facilities, faulty feeding habits, certain irrational belief systems and special tribal chores are likely to aggravate their health and nutritional status. Tribal women, in general, enjoy better status in the society than the general caste people because they exercise decisive role in the family (mother work), society (social work) and economy (other work) in India. However, the ideological devaluation of women’s contribution and reorientation of gender and sex have brought about concomitant drastic changes in the status of women and their empowerment in different dimensions of deprivation and exploitation, and imposed restrictions on daily folk-chores of life. In few communities, a definite decline has been observed threatening their very existence. This decline may not be due to low level of fertility but rather high level of mortality and illogical health practices. The success of dynamic tribal development is dependent on various factors like improved literacy rate, sustainable socio-economic status, women’s empowerment, better health care and other human resource indicators. It is much desirable to make reproductive health care accessible and affordable, extending basic amenities, empowering women and enhancing their employment opportunities, and providing the transport and communication facilities.
印度的部落社区经济和社会落后,大多生活在森林和丘陵地带,与其他精英社区隔绝。他们有自己的生活方式和不同的社会文化和生态地理环境。缺乏适当的教育和卫生设施、错误的饮食习惯、某些不合理的信仰体系和特殊的部落杂务可能使他们的健康和营养状况恶化。总的来说,部落妇女在社会上享有比一般种姓人更好的地位,因为她们在印度的家庭(母亲工作)、社会(社会工作)和经济(其他工作)中发挥着决定性的作用。然而,由于在意识形态上贬低妇女的贡献以及对性别和性的重新定位,随之而来的是妇女地位的急剧变化以及在剥夺和剥削的不同方面赋予她们权力,并对日常的民间生活琐事施加了限制。在少数社区,已经观察到明显的下降威胁到它们的生存。这种下降可能不是由于生育率低,而是由于死亡率高和不合理的保健做法。部落动态发展的成功取决于各种因素,如提高识字率、可持续的社会经济地位、赋予妇女权力、更好的保健和其他人力资源指标。使人们能够获得和负担得起生殖保健,扩大基本设施,赋予妇女权力和增加她们的就业机会,并提供交通和通讯设施,这是非常可取的。
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引用次数: 9
Sex Differences And Relationship Between Blood Pressure And Age Among The Ibos Of Nigeria 尼日利亚伊博人的性别差异及血压与年龄的关系
Pub Date : 2008-12-31 DOI: 10.5580/2cc
E. Jervase, D. Barnabas, A. Emeka, N. Osondu
Studies have established relationship between age and blood pressure (BP). The present study was carried out to establish normal BP values for the various age categories of both sexes and also to examine sex differences in BP within the various age groups of the Ibos. A random sampling of 563 Ibos of Nigeria aged 20 to 89 years, mean age 33.61 ± 13.61 were used for this study. The systolic blood pressure (SBP) and the diastolic blood pressure (DBP) were measured following standard protocol. The mean SBP and DBP of the study population was 119.63 ± 23.24 and 79.78 ± 12.80 respectively. When divided into seven age groups the analyses showed that both SBP and DBP increased with age. The lowest SBP was recorded in age 20 – 29 years in both sexes. The same was applicable for DBP. Generally men exhibited relatively high level of BP values, except for age groups 50 – 59 years and 60 – 69 years (SBP) where the mean BPs was slightly higher in the females. A significant difference was noted between DBP of males and females (p < 0.05) but none in the SBP. There was significant positive correlation (p < 0.01) of both SBP and DBP with age (r = 0.477 and 0.331 respectively). Our result is consistent with the findings that BP increases with age, with significant difference between males and females.
研究已经确定了年龄与血压(BP)之间的关系。本研究的目的是建立不同年龄组别的正常血压值,并检查不同年龄组别的血压的性别差异。随机抽取尼日利亚20 ~ 89岁ibo 563人,平均年龄33.61±13.61岁。按照标准方案测量收缩压(SBP)和舒张压(DBP)。研究人群的平均收缩压和舒张压分别为119.63±23.24和79.78±12.80。当被分为7个年龄组时,分析显示收缩压和舒张压都随着年龄的增长而增加。最低收缩压记录在20 - 29岁的男女。这同样适用于DBP。除50 ~ 59岁和60 ~ 69岁年龄组(SBP)中女性的平均BP略高外,男性一般表现出相对较高的血压值。男女舒张压差异有统计学意义(p < 0.05),收缩压差异无统计学意义(p < 0.05)。收缩压、舒张压与年龄呈显著正相关(r分别为0.477、0.331),p < 0.01。我们的研究结果与BP随年龄的增长而增加的发现是一致的,并且在男性和女性之间有显著差异。
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引用次数: 7
Frequency Of Agenesis Of The Palmaris Longus Muscle In Nigerians 尼日利亚人掌长肌发育不全的频率
Pub Date : 2008-12-31 DOI: 10.5580/cdc
S. Gabriel, C. Blessing, A.A.Henry Ugboma
This study was carried out to document the percentages of palmaris longus muscle amongst Nigeria for anthropological purposes.Three thousand (3,000) Nigerian subjects comprising 6000 extremities out of which 1932 (3864 extremities) were males and 1068 (2136 extremities) were females were inspected for the frequency of agenesis (absence) of the palmaris longus muscles. The presence or absence of palmaris longus was determined by physical examination of the palmaris longus tendon at the wrists of both hands. The muscle was made visible by opposing the thumb on the little finger and slightly flexing the wrist joint (in vivo examination). The frequency of agenesis of this muscle based on sex, side of the body and the overall incidence were determined. From the study, the muscle was found to be absent in 0.16% of the males on the right side and 0.19% of the females on the right side. On the left side, it was absent in 0.21% of males and 0.09% of females. On the average, percentage of agenesis was 0.18% in males and 0.14% in females. Thus the percentage was higher in males than females. The overall percentage of agenesis in Nigerians was 0.17%. This percentage is much lower than other populations. Bilateral absence was found in one male subject. There was no case of bilateral agenesis in females. The result is also reliable for anthropological studies involving Nigerians .It could be a useful guide to clinicians who may want to localize the median nerve amongst Nigerians.The tendon can still be counted on by surgeons treating Nigerian patients for use as a donor tendon, which will be present in a vast majority of Nigerian patients.
本研究是为了记录尼日利亚人掌长肌的百分比,用于人类学目的。三千(3000)名尼日利亚受试者,包括6000条肢体,其中1932(3864条)为男性,1068(2136条)为女性,检查掌长肌发育不全(缺失)的频率。手掌长肌的存在与否是通过体格检查双手手腕的手掌长肌肌腱来确定的。通过小指拇指的相对位置和轻微屈曲腕关节(体内检查)可以看到肌肉。根据性别、身体侧面和总体发生率确定该肌肉发育不全的频率。研究发现,0.16%的男性右侧肌肉缺失,0.19%的女性右侧肌肉缺失。在左侧,0.21%的男性和0.09%的女性没有。雄性和雌性平均发育不全率分别为0.18%和0.14%。因此,男性的比例高于女性。尼日利亚人发育不全的总体百分比为0.17%。这一比例远低于其他人群。在一名男性受试者中发现双侧缺失。女性无双侧发育不全病例。这一结果对于涉及尼日利亚人的人类学研究也是可靠的。对于那些想要定位尼日利亚人正中神经的临床医生来说,这可能是一个有用的指南。尼日利亚的外科医生在治疗病人时仍然可以将肌腱作为供体肌腱使用,绝大多数尼日利亚病人都会使用这种肌腱。
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引用次数: 3
Assessment of Ancestral Background from the Skull: Case Studies from Greece 从头骨评估祖先背景:来自希腊的案例研究
Pub Date : 2008-12-31 DOI: 10.5580/12d5
K. Moraitis, C. Eliopoulos, C. Spiliopoulou, S. Manolis
The assessment of ancestral background from human remains is an important aspect in biological and forensic anthropology. In the human skeleton, craniofacial morphology is considered the most reliable indicator of ancestral phenotype. In addition to craniofacial traits, anthropometric measurements may be employed in the assessment of ancestry. According to these, skeletal remains can be attributed to one of three population groups: Caucasoid, Negroid and Mongoloid. In this study the authors present examples of these population groups that they have encountered while working with human remains in Greece. The examples presented came from a forensic context and a human skeletal collection.
从人类遗骸中评估祖先背景是生物人类学和法医人类学的一个重要方面。在人类骨骼中,颅面形态被认为是祖先表型最可靠的指标。除了颅面特征外,人体测量还可用于评估祖先。根据这些,骨骼遗骸可以归因于三个人口群体之一:高加索人,黑人和蒙古人。在这项研究中,作者介绍了他们在希腊处理人类遗骸时遇到的这些人口群体的例子。所提出的例子来自法医背景和人类骨骼收集。
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引用次数: 5
期刊
The Internet Journal of Biological Anthropology
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