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PATHOLOGY OF EQUINE INFECTION WITH SALMONELLA TYPHIMPRIUM VAR COPENHAGEN 哥本哈根市马感染鼠伤寒沙门菌的病理分析
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1263
F.G. Eabasha, K.K Al- Awadi
Twelve horses were divided into three equal groups. Group I animals were inoculated orally with (3.7 X1012)CFU of a highly virulent strain of Salmonella typhimurium 3,;a_r cop§_n__hagen. Group 11 animals were inoculated orally with 2 doses of Salmonella and then challenged with a high dose. Group III horses, served as non - infected control. ‘ The main lesions were primarily confined to the digestive tract and were characterized by catarrhal enteritis. Pseudornembrane covered the mucosa of the ileum at the ileo — cecal Valve region. Histopathological examination revealed marked rnononuclear cellular infiltration in the lamina propria and occasionally necrosis and edema in the mucosa and submucosa of the intestine.
十二匹马被分成三组。用鼠伤寒沙门氏菌3的高毒力菌株的(3.7X1012)CFU口服接种I组动物,;a_r cop§_n__hagen。第11组动物口服2剂沙门氏菌,然后用高剂量攻击。第三组马,作为非感染对照主要病变主要局限于消化道,以卡他性肠炎为特征。回肠-盲肠瓣区的回肠粘膜上覆盖着假角膜膜。组织病理学检查显示固有层有明显的海绵状透明细胞浸润,肠粘膜和黏膜下层偶尔出现坏死和水肿。
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引用次数: 0
MICROBIOLOGICAL AND TREATMENT STUDIES OF OVINE FOOTROT INFECTION IN MOSUL AREA 摩苏尔地区绵羊足部感染的微生物学及治疗研究
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1240
M. Rhaymah, M. Sawa, Y. A. Yousif
It emerges from bacteriological study that it is possible to isolate many bacterial types and yeasts from the lesions of footrot infection in sheep. Anaerobic cultivation came out with the isolation of Spherophorus spp. (24%), Bacteroides spp. (60%), Corynebacterium spp. (64%), Enterobacteracae (76%), Streptococcus spp. (76%), Staphylococcus spp. (36%), Clostridum sordellii (46%) and Irichosporon cutaneum (4%).  On the other hand aerobic cultivation rendered the isolation of Corynebacterium spp. (100%), Entrobacteracae (100%), Staphylococcus spp. (15%), Penicillium spp., Aspergillus fumigatus and Trichosporon cutaneum.  Five different drug combinations were studied for their efficacy in the treatment of ovine footrot. Their healing rates were as follows : oxytetracycline (59%), oxytetracyclin with formaline (70.9%), Procaine penicillin and streptomycin (72.5%), Procaine penicillin and streptomycin with formaline dipping (80.76%), Formaline alone (63.8%). All kinds of treatment indicated statistically significant differences to exit between the treated (experimental) and untreated (control) groups
细菌学研究表明,从绵羊足腐病的病变中分离出多种细菌类型和酵母是可能的。厌氧培养分离出球藻属(24%)、拟杆菌属(60%)、棒状杆菌属(64%)、Enterobacteracae(76%)、链球菌属(76%),葡萄球菌属(36%),梭状芽孢杆菌属(46%)和皮肤虹彩菌属(4%)。另一方面,有氧培养可分离棒状杆菌属(100%)、入口杆菌属(100%)、葡萄球菌属(15%)、青霉属、烟曲霉和皮肤毛孢子菌。研究了五种不同药物组合对绵羊足腐病的治疗效果。其治愈率分别为:土霉素组(59%)、土霉素加甲林组(70.9%)、普鲁卡因-青霉素和链霉素组(72.5%)、普鲁卡因-青霉素-链霉素加甲林浸液组(80.76%)、福尔马林单独组(63.8%)
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引用次数: 0
CLINICAL, FAEMATOLOGICAL AND BIOCHEI‘./ECAL STUDIES ON ANAPLASMOSIS IN LOCAL BUFETALOES IN MOSUL, AREA 临床、药理学和生物化学/摩苏尔地区蟾蜍无浆体病的ECAL研究
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1260
K. M. Alsaad
Fourty five local buffaloes naturally infected with A. marginal age of 2-5 years. Clinical signs, present parasireamia haematological and biochemical changes were studied. Thin thick blood smears Stained with Gimsas stain were used to identify the parasite. The results indicated that most of the infected were suffering from mild form of infection and the 1I11p01”l11I1E' clinical signs observed were mild rise of body temperature, increase respiratory and plus rate, weal: ruminal contraction and generalized weakness. Palcness and iteration of mucous membrane, loss appetite, nasal and ocular discharge, coughing, salivation, atarcp, constipation, diarrhea, and rccumbancy. Statistical analysis showc increase (P<0.01) in body temperature, respiratory and pulsera wlflc nigrrificant decrease (P<0.01) in ruminal contraction We. observed. Parasiteamia in infected animals ranged from 1.3- 5.2%. Values of red blood cell, haemoglobin and packed cell volume were significantly low (P<0.01). Total leulcocyric and neutrophils count was significantly increase (P<0.0l). and (P<0.05) respectively. Blood indices values showed a significant increase in (MCHC) (P<0.0l) while (MCV) values showed no difference, reticulocytes were also present mildly biochemical changes indicated higher levels of AST. Total bilirubin (P<0.01). Total protein concentration was less (P<0.01) and no difference was observed inALT and blood urea nitrogen levels between infected and control animals.
45头当地水牛自然感染A.边缘年龄为2-5岁。研究了寄生虫病的临床表现、血液学和生化变化。采用Gimsas染色法进行薄血涂片鉴定。结果表明,大多数感染者为轻度感染,临床表现为轻度体温升高,呼吸和呼吸频率增加,良性:瘤胃收缩,全身无力。粘膜疼痛和反复,食欲不振,鼻和眼分泌物,咳嗽,流涎,鼻咽癌,便秘,腹泻和腹胀。统计分析显示,患者体温、呼吸、脉搏升高(P<0.01),瘤胃收缩显著降低(P<0.01)。观察到。受感染动物的寄生虫率为1.3- 5.2%。红细胞、血红蛋白和堆积细胞体积极显著降低(P<0.01)。总白细胞和中性粒细胞计数显著升高(P< 0.01)。和(P<0.05)。血液指标(MCHC)显著升高(P<0.01), MCV无显著差异,网织红细胞生化变化轻度,提示AST、总胆红素升高(P<0.01)。总蛋白浓度低于对照组(P<0.01), alt和尿素氮水平与对照组无显著差异。
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引用次数: 1
FREQUENCE OF JUXTAGLOMERULAR GRANULATED CELIS IN DEHYDRATED AND SODIUM-LOADED MICE 脱水和钠负荷小鼠肾小球旁颗粒细胞的频率
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1246
I. Al-ani, M. Homady
Fifteen albino mice were subjected to three days dehydration and fifteen albino mice were given 1% NaCl in their drinking water. Control animals were given drinking water  All animals were sacrificed and kidneys were fixed in different fixatives and processed for light microscopy  Section were stained for juxtaglomerular cells demonstration and the juxtaglomerular index (GI) and percentage of granulated nephron(%GN) were calculated. Kidneys from dehydrated and sodium-loaded animals showed significant decrease in both JGI and % GN and was discussed in relation to renin secretion and release.
对15只白化小鼠进行为期三天的脱水,并对15只白化病小鼠的饮用水中给予1%NaCl。给对照动物饮用水。处死所有动物,用不同的固定剂固定肾脏,并进行光学显微镜检查。对切片进行染色,以显示肾小球旁细胞,并计算肾小球旁指数(GI)和颗粒肾单位百分比(%GN)。脱水和钠负荷动物的肾脏JGI和%GN均显著降低,并与肾素分泌和释放有关。
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引用次数: 0
ULTRASTRUCTURAL CHANGES OF THE MESENTRIC LYMPH NODES OF CALVES EXPERIMENTALLY INFECTED WITH SALMONELLA TYPHIMURIUM 鼠伤寒沙门菌实验感染犊牛肠系膜淋巴结超微结构变化
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1247
Yass, A.W., H. F.G., A. S
A systemic study was done on the pathogenesis of experimentally induced Salmonella typhimurium infection in calves. The present investigation was carried out on sixteen normal colostrum fed friesian calves, ranging in age from 3 to 6 weeks. The calves were divided into two equal groups. Group I inoculated orally with (1.5 x 10'') Salmonella typhimurium and group IA served as control.  • The early ultrastructural alteration in the mesenteric lymph nodes was the presence of many free Salmonella in localized vacuoles. The interaction between the host cells and phagocytized Salmonella was also observed.
本文对实验性犊牛鼠伤寒沙门菌感染的发病机制进行了系统的研究。本研究对16头3 ~ 6周龄正常初乳喂养的弗里西亚犊牛进行了研究。小牛被分成两组。I组口服(1.5 × 10”)鼠伤寒沙门菌,IA组为对照组。•肠系膜淋巴结的早期超微结构改变是在局部液泡中存在许多游离沙门氏菌。还观察了宿主细胞与被吞噬的沙门氏菌之间的相互作用。
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引用次数: 0
SEROLOGICAL STUDY OF BOVINE LEUKOSIS IN IRAQ LOCAL BREED AND IMPORTED COW 伊拉克地方品种和进口奶牛白细胞病血清学研究
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1253
Afaf Abd - Al-Rahmain Yousif
Two hundreds and sixteen blood samples from Iraqi local breed cows in Baghdad, Basrah, Diala provinces and 144 blood samples from imported cows in three large cows stations were collected. All samples were tested by ELISA for the detection of specific antibody to bovine leucosis.  Results revealed the presence of antibodies of 8.4 % in imported cows only. Whereas the blood samples from the local animals included in this study were negative.
从巴格达、巴士拉和迪亚拉省的伊拉克当地品种奶牛身上采集了216份血液样本,并从三个大型奶牛站的进口奶牛身上收集了144份血液样本。所有样品均采用ELISA法检测牛白细胞病特异性抗体。结果显示,只有8.4%的抗体存在于进口奶牛中。而本研究中包括的当地动物的血液样本为阴性。
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引用次数: 0
BLOOD AND ACID-BASE CHANGES IN CAILVES EICPERIMENTALLY INFECTED WITH SALMONELLA TYPHIMPRIUM 鼠伤寒沙门菌实验感染犊牛血液及酸碱变化
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1262
F. Habasha, K. Michael, K.O. Al-Jebouril
The experimental design of this study was included (12) Friesian calves aged between 3-5 weeks. Calves were divided into two groups. Group I consisted of (8) calves were infected experimentally with 1.5 x 10“ of Salmonella typhimurium and often that treatment with chloramphenicol and electrolytes fluid were takes place. Group II consisted of (4) calves were infected with organisms but without treatment. The clinical findings of the disease were characterized by two forms which includes the septicemic and enteric forms. Haematological changes revealed a variable number of the total and differential leukocytic count, increased PCV, fibnnogen and decreased of the total plasma protein. . The changes in the acid-based balance indicate the development of metabolic acidosis.
本研究的实验设计包括(12)3-5周龄的弗里斯小牛。小牛被分成两组。第一组由(8)头小牛实验性感染1.5×10”鼠伤寒沙门氏菌,通常用氯霉素和电解质液体进行处理。第二组由(4)头小牛组成小牛感染了生物体,但未经治疗。该疾病的临床表现有两种形式,包括败血症和肠道形式。血液学变化显示,白细胞总数和差异计数各不相同,PCV、纤维蛋白原增加,血浆总蛋白减少。酸碱平衡的变化表明代谢性酸中毒的发展。
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引用次数: 0
EFFICACY OF IVERMACTIN AGANIST SOME NATURALLY ACQUIRED GASTRO-INTESTINAL PARASITIC INFECTION IN MULES 伊维菌素对骡子自然获得性胃肠道寄生虫感染的疗效观察
Pub Date : 2022-01-11 DOI: 10.30539/ijvm.v21i1.1249
Amir Saleem
The activity of a single subcutaneous injection of ivermectin at dose of 200 mcg/kg of body weight was evaluated against naturally acquired gastro-intestinal parasites in mules. Faecal samples were examined at the time of treatment and weekly thereafter up to 4 weeks. Results indicated that invrmectin was highly effective (100%) against Oxyuries equi, Strongyloides westeri, Tristostronglus axei, Trichhonema spp., and Strongylus spp. as judged one week after ivermectin administration.  Complete elimination of Parascaris equorum eggs occurred two weeks after treatment. In contrast, ivermectin was not effective against the tape worms, Anoplocephala spp.. Infected animals had low levels of haemoglobin and red blood cells counts and an elevated packed cell volume, white blood cells and erythrocyte sedimentation rate.  These values returned to normal values 21 days post treatment. No adverse reaction was recorded in the treated animals.
评估了以200 mcg/kg体重的剂量单次皮下注射伊维菌素对骡子自然获得性胃肠寄生虫的活性。在治疗时检查粪便样本,此后每周检查一次,直至4周。结果表明,伊维菌素给药一周后,invrmectin对马Oxuries equi、西氏Strongyloides westeri、axei Tristostronglus、Trichonema spp.和Strongylus spp.具有高度有效性(100%)。治疗两周后,雌马Parascaris equorum卵完全消失。相比之下,伊维菌素对扁头线虫无效。受感染的动物血红蛋白和红细胞计数较低,堆积细胞体积、白细胞和红细胞沉降率升高。这些值在治疗后21天恢复到正常值。在接受治疗的动物中未记录到不良反应。
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引用次数: 0
Prevalence of Hymenolepis spp. in House Mice in Baghdad City, Iraq 巴格达市家鼠膜膜绦虫流行病学调查
Pub Date : 2022-01-09 DOI: 10.30539/ijvm.v45i2.1259
Shaymaa A Majeed, A. Al-Amery
The prevalence of house mice (Mus musculus) Hymenolepiasis was determined in Baghdad, Iraq to study the effects of location, sex, and months on the infection rate of Hymenolepis spp. in house mice. Fifty house mice were captured from Abu Ghraib and Al-Ameriya, Baghdad, Iraq and examined for detecting parasites in laboratory in College of Veterinary Medicine, University of Baghdad. The total infection prevalence of intestinal parasites was 11 (22%) out of 50 samples, the higher prevalence was 28.57% and found in Abu Ghraib area, while lower was (13.63%) and recorded in Al-Ameriya area. The study revealed that the house mice were infected with two species of Hymenolepis: Hymenolepis nana 4 (8%) and Hymenolepis diminuta 7(14%). The intestinal parasites revealed a significance prevalence value (P<0.05). There was statistical difference in between males and females in parasites infection, in which the higher rate was 8 (27.58%) and recorded in males and the lower was 3 (14.28%) and observed in females. The monthly distribution of confirmed cases over a 9-month period revealed that reported cases of house mice and Hymenolepiasis increased significantly (P<0.01) in autumn (65.44%), followed by winter (15.38%), and summer (13.33%). The findings showed that house mice play an important role in the spread of zoonotic parasitic illnesses to people, as well as attention must pay to public health.
测定了伊拉克巴格达地区家鼠(小家鼠)膜膜绦虫的流行情况,探讨了地点、性别和月龄对家鼠膜膜绦虫感染率的影响。在伊拉克巴格达Abu Ghraib和Al-Ameriya捕获50只家鼠,在巴格达大学兽医学院实验室进行寄生虫检测。50份样本中肠道寄生虫总感染率为11例(22%),其中Abu Ghraib地区感染率最高,为28.57%,Al-Ameriya地区感染率较低,为13.63%。研究结果表明,家鼠感染两种膜膜绦虫:大膜膜绦虫4型(8%)和小膜膜绦虫7型(14%)。肠道寄生虫患病率显著(P<0.05)。男女寄生虫感染率差异有统计学意义,男性寄生虫感染率较高,为8例(27.58%),女性寄生虫感染率较低,为3例(14.28%)。9个月间的逐月确诊病例分布显示,家鼠和膜膜绦虫报告病例在秋季(65.44%)显著增加(P<0.01),其次是冬季(15.38%)和夏季(13.33%)。研究结果表明,家鼠在人畜共患寄生虫疾病向人类传播中起着重要作用,必须重视公共卫生。
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引用次数: 0
Use of Tannin-Containing Plants as Antimicrobials Influencing the Animal Health 含单宁植物抗菌剂对动物健康的影响
Pub Date : 2022-01-03 DOI: 10.30539/ijvm.v45i2.1258
M. M. Dakheel, Afnan A. AL-MNASER, J. Quijada, M. Woodward, C. Rymer
The antimicrobial effects of diverse tannin-containing plants, particularly condensed tannins (CTs) produced from various plants, are the subject of this study. CT components can be determined using CT-specific procedures such the HCl-Butanol Acetone assay, Thiolysis reaction, and HPLC/MS analysis. These methods indicate CT contents, including mean degree of polymerization, the procyanidins and prodelphinidins ratio (PC/PD%), the isomers of trans- and cis-, and CT concentration. Tannin-containing plants possess antibacterial action, which can be attributed to their protein linkage technique, and tannin-type variations, particularly CTs extract and their PC/PD%. The effects of CT components on the development of Gram-positive and Gram-negative bacteria have been documented for their relative PC/PD%; this is regarded to be a key predictor of tannin characteristics in terms of antimicrobials. In conclusion, tannins, more specific CT compositions, have significant impacts on in vivo trials of animal productions and utilization of metabolites and fermentation in vitro experiments. These findings need further investigations to fully understand how CT-types act on animal feeding in terms of enhanced nutritional quality of animal diets, which may have implications for human and animal health.
本研究的主题是含有多种单宁的植物,特别是由各种植物产生的缩合单宁(CT)的抗菌效果。CT组分可以使用CT特异性程序测定,如HCl丁醇丙酮测定、硫解反应和HPLC/MS分析。这些方法表明了CT含量,包括平均聚合度、原花青素和前花青素的比例(PC/PD%)、反式和顺式异构体以及CT浓度。含有单宁的植物具有抗菌作用,这可归因于它们的蛋白质连接技术和单宁类型的变化,特别是CTs提取物和它们的PC/PD%。CT成分对革兰氏阳性菌和革兰氏阴性菌发展的影响已记录为其相对PC/PD%;就抗菌剂而言,这被认为是单宁特性的关键预测因素。总之,单宁,更具体的CT成分,对动物生产的体内试验、代谢产物的利用和体外发酵实验具有重大影响。这些发现需要进一步的研究,以充分了解CT类型如何在提高动物饮食营养质量方面对动物喂养产生作用,这可能对人类和动物健康产生影响。
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引用次数: 2
期刊
The Iraqi Journal of Veterinary Medicine
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