Mohammad Arfi Setiawan, Mita Dewi Retnoningrum, Febriyandhi Yahya, Resa Ragil Andika, D. H. A. Sudarni
Antibacterial Activity of Citrus seed (Citrus reticulata) Extract on Escherichia coli Indonesian agriculture provides a resource of medicinal plants whose potential needs to be explored in order to benefit society. One of them is the use of Siam orange seeds (Citrus reticulata) which has the potential for the production of antibacterial compounds. This study aims to test the antibacterial activity of the ethanol and n-hexane extract of orange seeds. The extract was obtained through maceration techniques using ethanol and n-hexane as solvents. The antibacterial activity test of orange seeds against Escherichia coli used the paper disc diffusion method with nutrient agar (NA) media. The concentration of orange seed extract for the determination of MIC (Minimum Inhibitory Concentration) was 0.5, 2, 8, 10, 20 mg mL-1. The results showed that the ethanol and n-hexane extract of orange seeds had antibacterial activity against E. coli. However, the ethanol extract had a higher antibacterial effect than the n-hexane orange seed extract. From the results of this study, it is hoped that the waste of orange seeds will provide beneficial contribution for pharmaceutical development. Pertanian Indonesia memiliki sumber tanaman obat yang perlu digali potensinya agar bermanfaat bagi masyarakat. Salah satunya pemanfaatan biji jeruk siam (Citrus reticulata) yang berpotensi menghasilkan senyawa antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak etanol dan n-heksana biji jeruk. Ekstrak diperoleh melalui teknik maserasi menggunakan pelarut etanol dan n-heksana. Uji aktivitas antibakteri biji jeruk terhadap Escherichia coli menggunakan metode difusi paper disc dengan media nutrient agar (NA). Konsentrasi ekstrak biji jeruk untuk penentuan MIC (Minimum Inhibitory Concentration) adalah 0,5, 2, 8, 10, 20 mg mL-1. Hasil penelitian menunjukkan bahwa ekstrak etanol dan n-heksana biji jeruk memiliki aktivitas antibakteri terhadap E. coli. Namun, ekstrak etanol memiliki efek antibakteri yang lebih tinggi dibandingkan ekstrak biji jeruk n-heksana. Dari hasil penelitian ini, limbah biji jeruk diharapkan dapat memberikan kontribusi bermanfaat bagi pengembangan farmasi.
柑桔种子(Citrus reticulata)提取物对大肠杆菌的抑菌活性印度尼西亚农业提供了一种药用植物资源，其潜力有待开发以造福社会。其中之一是使用暹罗橙种子(Citrus reticulata)，它具有生产抗菌化合物的潜力。本研究旨在测试橙籽乙醇和正己烷提取物的抑菌活性。以乙醇和正己烷为溶剂，通过浸渍法获得提取液。采用营养琼脂(NA)培养基纸盘扩散法对柑桔种子进行了抑菌试验。橙籽提取物的MIC (Minimum Inhibitory concentration)测定浓度分别为0.5、2、8、10、20 mg mL-1。结果表明，橙籽乙醇和正己烷提取物对大肠杆菌具有一定的抑菌活性。乙醇提取物的抑菌效果优于正己烷提取物。从本研究的结果来看，希望利用废弃的橘子籽为药物开发提供有益的贡献。Pertanian Indonesia memoriliki sumber tanaman obat yang perlu digali potensinya agar bermanfaat bagi masyarakat。柑桔(柑桔)抗虫菌。[中文]:Penelitian, bertujuan, untuk, menguji, aktivas, anti - bakterak, etaket，乙醇，乙醇。生产工艺条件:生产工艺条件、生产工艺条件、生产工艺条件、生产工艺条件、生产工艺条件。抑菌活性、抑菌活性、抑菌活性、抑菌活性、抑菌活性、抑菌活性、抑菌活性、抑菌活性、抑菌活性。最小抑制浓度:0、5、2、8、10、20 mg mL-1。对大肠杆菌的抑菌活性研究。抗细菌，抗细菌，抗细菌，抗细菌，抗细菌，抗细菌，抗细菌，抗细菌，抗细菌，抗细菌，抗细菌。达里哈西尔penelitian ini，林巴比吉jeruk diharapkan dapat成员，kontribusi bermanfaat bagi pengembangan farmasi。
{"title":"AKTIVITAS ANTIBAKTERI EKSTRAK BIJI JERUK SIAM (Citrus reticulata) PADA BAKTERI Escherichia coli","authors":"Mohammad Arfi Setiawan, Mita Dewi Retnoningrum, Febriyandhi Yahya, Resa Ragil Andika, D. H. A. Sudarni","doi":"10.29122/JBBI.V7I2.4194","DOIUrl":"https://doi.org/10.29122/JBBI.V7I2.4194","url":null,"abstract":"Antibacterial Activity of Citrus seed (Citrus reticulata) Extract on Escherichia coli\u0000Indonesian agriculture provides a resource of medicinal plants whose potential needs to be explored in order to benefit society. One of them is the use of Siam orange seeds (Citrus reticulata) which has the potential for the production of antibacterial compounds. This study aims to test the antibacterial activity of the ethanol and n-hexane extract of orange seeds. The extract was obtained through maceration techniques using ethanol and n-hexane as solvents. The antibacterial activity test of orange seeds against Escherichia coli used the paper disc diffusion method with nutrient agar (NA) media. The concentration of orange seed extract for the determination of MIC (Minimum Inhibitory Concentration) was 0.5, 2, 8, 10, 20 mg mL-1. The results showed that the ethanol and n-hexane extract of orange seeds had antibacterial activity against E. coli. However, the ethanol extract had a higher antibacterial effect than the n-hexane orange seed extract. From the results of this study, it is hoped that the waste of orange seeds will provide beneficial contribution for pharmaceutical development.\u0000Pertanian Indonesia memiliki sumber tanaman obat yang perlu digali potensinya agar bermanfaat bagi masyarakat. Salah satunya pemanfaatan biji jeruk siam (Citrus reticulata) yang berpotensi menghasilkan senyawa antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak etanol dan n-heksana biji jeruk. Ekstrak diperoleh melalui teknik maserasi menggunakan pelarut etanol dan n-heksana. Uji aktivitas antibakteri biji jeruk terhadap Escherichia coli menggunakan metode difusi paper disc dengan media nutrient agar (NA). Konsentrasi ekstrak biji jeruk untuk penentuan MIC (Minimum Inhibitory Concentration) adalah 0,5, 2, 8, 10, 20 mg mL-1. Hasil penelitian menunjukkan bahwa ekstrak etanol dan n-heksana biji jeruk memiliki aktivitas antibakteri terhadap E. coli. Namun, ekstrak etanol memiliki efek antibakteri yang lebih tinggi dibandingkan ekstrak biji jeruk n-heksana. Dari hasil penelitian ini, limbah biji jeruk diharapkan dapat memberikan kontribusi bermanfaat bagi pengembangan farmasi.","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128841035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. R. P. Wardoyo, Widya Anggraeni, .. Rahmawati, H. A. Oramahi
Antifungal Activity of Wood Vinegar derived from Oil Palm Empty Bunches against Colletotrichum sp. (WA2) Colletotrichum sp. is a fungus that causes anthracnose in cayenne pepper (Capsicum frutescens L.). An alternative natural control for this fungus is using wood vinegar. The aim of this study was to evaluate antifungal properties of wood vinegar from oil palm empty bunches (Elaeis guineensis Jacq.) against Colletotrichum sp. The antifungal test was carried out using the solid dilution method by poisoning food in potato dextrose agar (PDA) media. Colletotrichum sp. used was isolated from red chili which had anthracnose symptoms. This experimental research method used a completely randomized design (CRD) with 9 treatments, namely, negative control (non-liquid smoke), positive control (Dithane M45 at 0.20%), liquid smoke concentration of 0.40; 0.42; 0.44; 0.46; 0.48; 0.50; and 0.52%. Anova test results showed wood vinegar from empty fruit bunches of E. guineensis at concentrations of 0.40; 0.42; 0.44; 0.46 and 0.48% differed significantly from negative control, positive control, and concentrations of 0.50 and 0.52%. The concentration of 0.42% was the minimum inhibitory concentration with an average value of 87.98% inhibition and a very strong activity level. In conclusion, the wood vinegar from E. guineensis can be used to control Colletotrichum sp fungus at an effective concentration of 0.42%. Colletotrichum sp. merupakan jamur penyebab penyakit antraknosa pada tanaman cabai rawit (Capsicum frutescens L.). Salah satu alternatif pengendalian secara alami terhadap jamur tersebut adalah dengan menggunakan asap cair. Penelitian ini bertujuan untuk mengevaluasi sifat antijamur asap cair dari tandan kosong Elaeis guineensis Jacq (kelapa sawit) terhadap Colletotrichum sp. Uji antijamur dilakukan dengan metode dilusi padat melalui cara poisoning food dalam media potato dextrose agar (PDA). Isolat jamur Colletotrichum sp. yang digunakan merupakan hasil isolasi dari tanaman cabe merah yang bergejala antraknosa. Metode penelitian ini mengunakan rancangan acak lengkap (RAL) dengan 9 perlakuan, yaitu kontrol negatif (tanpa asap cair), kontrol positif (Dithane M45 sebesar 0,20%), konsentrasi asap cair sebesar 0,40; 0,42; 0,44; 0,46; 0,48; 0,50; dan 0,52%. Hasil uji Anova menunjukkan asap cair dari tandan kosong E. guineensis pada konsentrasi 0,40; 0,42; 0,44; 0,46; dan 0,48% berbeda secara signifikan dengan kontrol negatif, kontrol positif dan konsentrasi 0,50 dan 0,52%. Konsentrasi 0,42% merupakan konsentrasi hambat minimum dengan nilai rata-rata penghambatan sebesar 87,98% dan tingkat aktivitas sangat kuat. Dengan demikian dapat disimpulkan bahwa asap cair dari tandan kosong E. guineensis dapat digunakan untuk mengendalikan jamur Colletotrichum sp. pada konsentrasi efektif 0,42%.
油棕空枝木醋对炭疽菌(Colletotrichum sp.， WA2)的抑菌活性研究炭疽菌是一种引起辣椒炭疽病的真菌。对这种真菌的另一种自然控制方法是使用木醋。采用固体稀释法，在马铃薯葡萄糖琼脂(PDA)培养基中投毒食物，研究了油棕空枝木醋对炭疽病菌的抑菌效果。所用炭疽病菌是从具有炭疽病症状的红辣椒中分离得到的。本试验研究方法采用完全随机设计(CRD)，共设9个处理，即阴性对照(无液体烟雾)、阳性对照(乙烷M45浓度为0.20%)，液体烟雾浓度为0.40;0.42;0.44;0.46;0.48;0.50;和0.52%。方差分析结果显示，豚鼠空果束木醋浓度为0.40;0.42;0.44;0.46和0.48%与阴性对照、阳性对照、0.50和0.52%浓度差异显著。0.42%的浓度为最低抑制浓度，平均抑制率为87.98%，活性水平很强。综上所述，豚鼠木醋液在0.42%的有效浓度下可有效防治炭疽菌。辣椒炭疽菌属(merupakan)萨拉赫是彭根达斯的替代者，他认为这是一场非常激烈的比赛，但他认为这是一场非常激烈的比赛。摘要:马铃薯葡萄糖琼脂(PDA)是一种有毒的食物培养基，马铃薯葡萄糖琼脂(PDA)是一种有毒的食物培养基。杨氏炭疽菌属。杨氏炭疽菌属。杨氏炭疽菌属。方法penelitian ini mengunakan ranancan acak lengkap (RAL) dengan 9 perlakuan, yaitu对照阴性(tanpa asap cair)，对照阳性(Dithane M45 sebesar 0,20%)， konsentrasi asap cair sebesar 0,40;0, 42岁;0, 44岁;0, 46个;0, 48岁;0, 50;丹·0 52%。[j] .黑龙江农业大学学报(自然科学版);0, 42岁;0, 44岁;0, 46个;对照阴性丹参0、50丹参0、52%，对照阴性丹参0、50丹参0、52%。【翻译】Konsentrasi 0,42% merupakan Konsentrasi hambat minimum dengan nilai rata-rata penghambatan sebesar 87,98% dan tingkat aktivitas sangat kuat。Dengan demikian dapat dispulkan bahwa asap cair dari tandan kosong E. guineensis dapat digunakan untuk mengendalikan jamur Colletotrichum sp. pagadkonsentrasi efektif 0,42%。
{"title":"AKTIVITAS ANTIFUNGI ASAP CAIR DARI TANDAN KOSONG Elaeis guineensis Jacq.TERHADAP Colletotrichum sp. (WA2)","authors":"E. R. P. Wardoyo, Widya Anggraeni, .. Rahmawati, H. A. Oramahi","doi":"10.29122/JBBI.V7I2.3582","DOIUrl":"https://doi.org/10.29122/JBBI.V7I2.3582","url":null,"abstract":"Antifungal Activity of Wood Vinegar derived from Oil Palm Empty Bunches against Colletotrichum sp. (WA2)\u0000Colletotrichum sp. is a fungus that causes anthracnose in cayenne pepper (Capsicum frutescens L.). An alternative natural control for this fungus is using wood vinegar. The aim of this study was to evaluate antifungal properties of wood vinegar from oil palm empty bunches (Elaeis guineensis Jacq.) against Colletotrichum sp. The antifungal test was carried out using the solid dilution method by poisoning food in potato dextrose agar (PDA) media. Colletotrichum sp. used was isolated from red chili which had anthracnose symptoms. This experimental research method used a completely randomized design (CRD) with 9 treatments, namely, negative control (non-liquid smoke), positive control (Dithane M45 at 0.20%), liquid smoke concentration of 0.40; 0.42; 0.44; 0.46; 0.48; 0.50; and 0.52%. Anova test results showed wood vinegar from empty fruit bunches of E. guineensis at concentrations of 0.40; 0.42; 0.44; 0.46 and 0.48% differed significantly from negative control, positive control, and concentrations of 0.50 and 0.52%. The concentration of 0.42% was the minimum inhibitory concentration with an average value of 87.98% inhibition and a very strong activity level. In conclusion, the wood vinegar from E. guineensis can be used to control Colletotrichum sp fungus at an effective concentration of 0.42%.\u0000Colletotrichum sp. merupakan jamur penyebab penyakit antraknosa pada tanaman cabai rawit (Capsicum frutescens L.). Salah satu alternatif pengendalian secara alami terhadap jamur tersebut adalah dengan menggunakan asap cair. Penelitian ini bertujuan untuk mengevaluasi sifat antijamur asap cair dari tandan kosong Elaeis guineensis Jacq (kelapa sawit) terhadap Colletotrichum sp. Uji antijamur dilakukan dengan metode dilusi padat melalui cara poisoning food dalam media potato dextrose agar (PDA). Isolat jamur Colletotrichum sp. yang digunakan merupakan hasil isolasi dari tanaman cabe merah yang bergejala antraknosa. Metode penelitian ini mengunakan rancangan acak lengkap (RAL) dengan 9 perlakuan, yaitu kontrol negatif (tanpa asap cair), kontrol positif (Dithane M45 sebesar 0,20%), konsentrasi asap cair sebesar 0,40; 0,42; 0,44; 0,46; 0,48; 0,50; dan 0,52%. Hasil uji Anova menunjukkan asap cair dari tandan kosong E. guineensis pada konsentrasi 0,40; 0,42; 0,44; 0,46; dan 0,48% berbeda secara signifikan dengan kontrol negatif, kontrol positif dan konsentrasi 0,50 dan 0,52%. Konsentrasi 0,42% merupakan konsentrasi hambat minimum dengan nilai rata-rata penghambatan sebesar 87,98% dan tingkat aktivitas sangat kuat. Dengan demikian dapat disimpulkan bahwa asap cair dari tandan kosong E. guineensis dapat digunakan untuk mengendalikan jamur Colletotrichum sp. pada konsentrasi efektif 0,42%.","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121702763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Appendix JBBI Vol 6, No 1, June 2019: Keyword Index and Author Index","authors":"Catur Sriherwanto","doi":"10.29122/jbbi.v6i1.3692","DOIUrl":"https://doi.org/10.29122/jbbi.v6i1.3692","url":null,"abstract":"","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128743875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bedah Rupaedah, D. Purwoko, Anna Safarrida, Teuku Tajuddin, A. Wahid, Mahmud Sugianto, Imam Sudjai, Agus Suyono
Screening and Identification of Ligninolytic Microbes in the Natural Decomposition of Oil Palm Empty Fruit Bunch ABSTRACTOPEFB (oil palm empty fruit bunch)could potentially be utilized as organic fertilizer or animal feed through composting. Information on microorganisms that play important roles in the natural decomposition of OPEFB is to date not much known yet. This research was aimed to obtain and, subsequently, to molecularly identify lignin-degrading microbial isolates responsible for naturally decomposing OPEFB in the Oil Plant Plantation and Palm Oil Refinery Plant, PTPN VIII Cikasungka, Bogor. Screening for active lignin-degrading isolates was carried out on 17 naturally decomposing OPEFB samples. A total of 19 isolates of fungi and 80 isolates of bacteria were obtained. Ligninolytic activity was measured by Sundman and Nase testing methods. Ligninolytic activity was found on 13 fungal isolates and 15 bacterial isolates. The active isolates were subsequently identified molecularly based on ITS sequence in the ribosome DNA area for fungi and in 16S rRNA genes for bacteria. The results showed that the lignin-degrading microorganisms obtained consisted of 5 bacterial isolates from the genus Bacillus and 3 fungal isolates from the genus Rhizopus and Aspergillus. Keywords: composting, lignin, microbes, OPEFB, 16S rRNA ABSTRAKTKKS (tandan kosong kelapa sawit) berpotensi dimanfaatkan sebagai pupuk organik atau pakan ternak dengan cara pengomposan. Informasi mikroba yang berperan dalam pengomposan alami TKKS hingga saat ini belum banyak diketahui. Penelitian ini bertujuan mendapatkan isolat mikroba pendegradasi lignin dalam pengomposan alami TKKS asal Perkebunan dan Pabrik Pemerasan Kelapa Sawit, PTPN VIII Cikasungka, Bogor, serta mengidentifikasi mikroba tersebut secara molekuler. Skrining mikroba aktif pendegradasi lignin dilakukan terhadap 17 sampel TKKS yang sudah lapuk secara alami. Sebanyak 19 isolat jamur dan 80 isolat bakteri telah dihasilkan. Aktivitas ligninolitik diukur dengan metode pengujian Sundman dan Nase. Isolat jamur yang memiliki aktivitas ligninolitik sebanyak 13 isolat, sedangkan bakteri sebanyak 15 isolat. Isolat-isolat aktif tersebut selanjutnya diidentifikasi secara molekuler berdasarkan pada sekuen ITS di daerah DNA ribosom untuk jamur dan menggunakan gen 16S rRNA untuk bakteri. Hasil menunjukkan bahwa 5 isolat bakteri yang memiliki kemampuan mendegradasi lignin berasal dari genus Bacillus, sedangkan 3 isolat jamur pendegradasi lignin berasal dari genus Rhizopus dan Aspergillus Kata Kunci: lignin, mikroba, pengomposan, TKKS, 16S rRNA
摘要油棕空果串天然分解木质素降解微生物的筛选与鉴定油棕空果串堆肥可作为有机肥或动物饲料利用。关于在OPEFB自然分解中起重要作用的微生物的信息迄今为止还不太清楚。这项研究的目的是获得并随后分子鉴定木质素降解微生物分离物，这些微生物分离物负责自然分解油料植物种植园和棕榈油精炼厂，PTPN VIII Cikasungka，茂物。对17个自然分解的OPEFB样品进行了活性木质素降解分离菌株的筛选。共分离真菌19株，细菌80株。采用Sundman法和Nase法测定木质素分解活性。在13株真菌和15株细菌分离物中发现木质素分解活性。随后，根据真菌核糖体DNA区域和细菌16S rRNA基因的ITS序列对活性分离物进行了分子鉴定。结果表明，获得的木质素降解微生物包括5株芽孢杆菌属细菌和3株根霉属真菌和曲霉属真菌。关键词:堆肥，木质素，微生物，OPEFB, 16S rRNA (tandan kosong kelapa sawit) berpotensi dimanfaatkan sebagai pupuk organik atau pakan ternak dengan cara pengomposan)资料来源:中国科学院，中国科学院，中国科学院，中国科学院，中国科学院。Penelitian ini bertujuan mendapatkan isolat mikroba pendegradasi lignin dalam pengomposan alami TKKS asal Perkebunan dan Pabrik Pemerasan Kelapa Sawit, PTPN VIII Cikasungka, Bogor, serta mengidentifikasi mikroba tersebut secara分子。skrba aktif pen降解木质素dilakukan terhadap 17样品TKKS yang sudah lapuksecara alami。Sebanyak 19 isolat jamur dan 80 isolat bakteri telah dihasilkan。木质素木质素活树的研究。木质素olitik sebanyak 13, sedangkan bakteri sebanyak 15。分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物-分离物Hasil menunjukkan bahwa 5 isolat bakteri yang memiliki kemampuan men降解木质素berasal dari属芽孢杆菌，sedangkan 3 isolat jamur pen降解木质素berasal dari属Rhizopus dan Aspergillus Kata Kunci:木质素，mikroba, pengomposan, TKKS, 16S rRNA
{"title":"SKRINING DAN IDENTIFIKASI MIKROBA LIGNINOLITIK PADA PENGOMPOSAN ALAMI TANDAN KOSONG KELAPA SAWIT","authors":"Bedah Rupaedah, D. Purwoko, Anna Safarrida, Teuku Tajuddin, A. Wahid, Mahmud Sugianto, Imam Sudjai, Agus Suyono","doi":"10.29122/JBBI.V6I1.3237","DOIUrl":"https://doi.org/10.29122/JBBI.V6I1.3237","url":null,"abstract":"Screening and Identification of Ligninolytic Microbes in the Natural Decomposition of Oil Palm Empty Fruit Bunch ABSTRACTOPEFB (oil palm empty fruit bunch)could potentially be utilized as organic fertilizer or animal feed through composting. Information on microorganisms that play important roles in the natural decomposition of OPEFB is to date not much known yet. This research was aimed to obtain and, subsequently, to molecularly identify lignin-degrading microbial isolates responsible for naturally decomposing OPEFB in the Oil Plant Plantation and Palm Oil Refinery Plant, PTPN VIII Cikasungka, Bogor. Screening for active lignin-degrading isolates was carried out on 17 naturally decomposing OPEFB samples. A total of 19 isolates of fungi and 80 isolates of bacteria were obtained. Ligninolytic activity was measured by Sundman and Nase testing methods. Ligninolytic activity was found on 13 fungal isolates and 15 bacterial isolates. The active isolates were subsequently identified molecularly based on ITS sequence in the ribosome DNA area for fungi and in 16S rRNA genes for bacteria. The results showed that the lignin-degrading microorganisms obtained consisted of 5 bacterial isolates from the genus Bacillus and 3 fungal isolates from the genus Rhizopus and Aspergillus. Keywords: composting, lignin, microbes, OPEFB, 16S rRNA ABSTRAKTKKS (tandan kosong kelapa sawit) berpotensi dimanfaatkan sebagai pupuk organik atau pakan ternak dengan cara pengomposan. Informasi mikroba yang berperan dalam pengomposan alami TKKS hingga saat ini belum banyak diketahui. Penelitian ini bertujuan mendapatkan isolat mikroba pendegradasi lignin dalam pengomposan alami TKKS asal Perkebunan dan Pabrik Pemerasan Kelapa Sawit, PTPN VIII Cikasungka, Bogor, serta mengidentifikasi mikroba tersebut secara molekuler. Skrining mikroba aktif pendegradasi lignin dilakukan terhadap 17 sampel TKKS yang sudah lapuk secara alami. Sebanyak 19 isolat jamur dan 80 isolat bakteri telah dihasilkan. Aktivitas ligninolitik diukur dengan metode pengujian Sundman dan Nase. Isolat jamur yang memiliki aktivitas ligninolitik sebanyak 13 isolat, sedangkan bakteri sebanyak 15 isolat. Isolat-isolat aktif tersebut selanjutnya diidentifikasi secara molekuler berdasarkan pada sekuen ITS di daerah DNA ribosom untuk jamur dan menggunakan gen 16S rRNA untuk bakteri. Hasil menunjukkan bahwa 5 isolat bakteri yang memiliki kemampuan mendegradasi lignin berasal dari genus Bacillus, sedangkan 3 isolat jamur pendegradasi lignin berasal dari genus Rhizopus dan Aspergillus Kata Kunci: lignin, mikroba, pengomposan, TKKS, 16S rRNA ","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"268 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116396030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman. Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik
摘要蔗糖磷酸合酶(SPS)是参与蔗糖生物合成的关键酶。在调控过程中，SPS活性由一种变构效应物葡萄糖-6-磷酸(G6P)调节，该变构效应物被认为具有结合SPS n端结构域的能力。为了了解n端在SPS调控中的作用，我们对SPS基因进行突变，缺失n端结构域(∆N-SPS)。将∆N-SPS转化为番茄植株，转化效率为5%。获得3株转基因番茄植株4.20、5.5.1和5.10，经PCR分析证实。通过酶分析对转基因番茄的表达进行了表征。结果表明，转基因植株中G6P的变构调节丧失，SPS活性比非转基因植株提高2倍。这表明n端结构域缺失的SPS能够在植物中积极表达。关键词:酶，遗传转化，n端结构域缺失，蔗糖磷酸合成酶，番茄Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada结构域n端SPS。Untuk mengetahui peran n端pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan域n端(∆N-SPS)。Gen∆N-SPS diinsersi paada tanaman番茄千层转化基因(melalui transformasi genetick dengan efisiensi transformasi 5%)。Tiga tanaman转基因番茄(event4.20;5.5.1;(5.10) didapatkan dan terkonfirmasi melalal分析PCR阳性。白藜芦醇的三聚氰胺分析。Hasil menunjukkan bahwa tanaman tomgenik (N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik。n -端结构域，n -端结构域，n -端结构域，n -端结构域，n -端结构域。Kata Kunci: delesi结构域n端，酶，蔗糖磷酸合酶，番茄，转化基因
{"title":"TRANSFORMASI GENETIK DAN EKSPRESI MUTAN SUCROSE PHOSPHATE SYNTHASE PADA TANAMAN TOMAT","authors":"Suwinda Fibriani, Inyana Dwi Agustien, Widhi Dyah Sawitri, Bambang Sugiharto","doi":"10.29122/JBBI.V6I1.3341","DOIUrl":"https://doi.org/10.29122/JBBI.V6I1.3341","url":null,"abstract":"Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman. Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik ","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116468914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Comparison of Three RNA Extraction Kits for Transcriptome Analysis of Oil Palm (Elaeis guineensis Jacq.) ABSTRACTObtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). The aim of this study was to obtain RNA extraction methods on oil palm leaves, callus and somatic embryos that were of good quality and high concentrations for transcriptomic analysis. RNA extraction was carried out using Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) and RibospinTM Plant (Geneall) kit methods. The results showed that oil palm leaf, callus and somatic embryo RNA were successfully extracted using the RibospinTM (Geneall) kit. Based on the total RNA number of more than 4 μg and the RIN value of more than 7, the extracted RNA could be used in RNA sequencing for transcriptomic analysis. Keywords: callus, oil palm, RNA analysis, RNA quality, somatic embryo ABSTRAKMenghasilkan RNA berkualitas tinggi sangatlah penting pada tahap awal penelitian biologi molekuler. Untuk mengisolasi RNA diperlukan keterampilan dan kehati-hatian tinggi dalam mengikuti prosedur di laboratorium karena RNA lebih mudah terdegradasi, khususnya sampel hasil kultur jaringan tanaman. Salah satu parameter yang digunakan pada pengecekan kualitas RNA total adalah RIN (RNA Integrity Number). Penelitian bertujuan mendapatkan metode ekstraksi RNA pada daun, kalus dan embrio somatik kelapa sawit yang berkualitas baik dan memiliki konsentrasi tinggi untuk analisa transkriptomika. Ekstraksi RNA dilakukan menggunakan metode kit Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) dan RibospinTM Plant (Geneall). Hasil menunjukkan bahwa RNA daun, kalus dan embrio somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM (Geneall). RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan analisis transkriptomika, dilihat dari jumlah total RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7. Kata Kunci: analisis RNA, embrio somatic, kalus, kelapa sawit, kualitas RNA
三种RNA提取试剂盒用于油棕转录组分析的比较在分子生物学研究的早期阶段，获得高质量的RNA是非常重要的。为了分离RNA，在遵循实验室程序时需要很高的技巧和谨慎，因为RNA很容易降解，特别是来自植物组织培养的样本。用于检查总RNA质量的参数之一是RIN (RNA完整性数)。本研究的目的是获得高质量、高浓度的油棕叶片、愈伤组织和体细胞胚RNA提取方法，用于转录组学分析。采用Plant RNA PureLink (Ambion)、Genezol RNA extraction (Geneaid)和RibospinTM Plant (Geneall)试剂盒进行RNA提取。结果表明，利用RibospinTM (Geneall)试剂盒成功提取了油棕叶、愈伤组织和体胚RNA。总RNA数大于4 μg, RIN值大于7，可用于RNA测序进行转录组学分析。关键词:愈伤组织，油棕，RNA分析，RNA质量，体胚Untuk mengisolasi RNA diperlukan keterampilan dan kehatian tingi dalam mengikuti proseri laboratorium karena RNA lebih mudah ter降解，khususnya样本hasil kuljaringan和tanaman。Salah satu parameter yang digunakan padpengecekan kualitas RNA total adalah RIN (RNA完整性数)。Penelitian bertujuan mendapatkan mede ekstraksi RNA - dadan, kalus和胚胎体细胞- kelapk - sawit yang berkualitas baik - memiliki konsentrasi tinggi untuk analisa transkritomika。Ekstraksi RNA dilakkan menggunakan metode kit Plant RNA PureLink (Ambion)， Genezol RNA Extraction (Geneaid)和RibospinTM Plant (generall)。Hasil menunjukkan bahwa RNA daun, kalus和胚胎somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM(通用)。RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan分析转录组学，dilihat dari jumlah总RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7。Kata Kunci:分析RNA，胚胎体细胞，kalus, kelapa sawit, kualitas RNA
{"title":"PERBANDINGAN TIGA KIT EKSTRAKSI RNA UNTUK ANALISIS TRANSKRIPTOMIKA PADA KELAPA SAWIT (Elaeis guineensis Jacq.)","authors":"Siti Zulaeha, Devit Purwoko, Imam Civi Cartealy, Teuku Tajuddin, .. Karyanti, H. Khairiyah","doi":"10.29122/JBBI.V6I1.3372","DOIUrl":"https://doi.org/10.29122/JBBI.V6I1.3372","url":null,"abstract":"Comparison of Three RNA Extraction Kits for Transcriptome Analysis of Oil Palm (Elaeis guineensis Jacq.) ABSTRACTObtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). The aim of this study was to obtain RNA extraction methods on oil palm leaves, callus and somatic embryos that were of good quality and high concentrations for transcriptomic analysis. RNA extraction was carried out using Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) and RibospinTM Plant (Geneall) kit methods. The results showed that oil palm leaf, callus and somatic embryo RNA were successfully extracted using the RibospinTM (Geneall) kit. Based on the total RNA number of more than 4 μg and the RIN value of more than 7, the extracted RNA could be used in RNA sequencing for transcriptomic analysis. Keywords: callus, oil palm, RNA analysis, RNA quality, somatic embryo ABSTRAKMenghasilkan RNA berkualitas tinggi sangatlah penting pada tahap awal penelitian biologi molekuler. Untuk mengisolasi RNA diperlukan keterampilan dan kehati-hatian tinggi dalam mengikuti prosedur di laboratorium karena RNA lebih mudah terdegradasi, khususnya sampel hasil kultur jaringan tanaman. Salah satu parameter yang digunakan pada pengecekan kualitas RNA total adalah RIN (RNA Integrity Number). Penelitian bertujuan mendapatkan metode ekstraksi RNA pada daun, kalus dan embrio somatik kelapa sawit yang berkualitas baik dan memiliki konsentrasi tinggi untuk analisa transkriptomika. Ekstraksi RNA dilakukan menggunakan metode kit Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) dan RibospinTM Plant (Geneall). Hasil menunjukkan bahwa RNA daun, kalus dan embrio somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM (Geneall). RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan analisis transkriptomika, dilihat dari jumlah total RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7. Kata Kunci: analisis RNA, embrio somatic, kalus, kelapa sawit, kualitas RNA ","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115484089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. A. Paramadini, Catur Sriherwanto, Nurlaila, I. Suja’i, M. Annisa
Influence of Biofloatation Technique through Rhizopus sp. Fermentation on the Nutritional Quality of the Floating Fish FeedABSTRACTSolid fermentation using the mold Rhizopus has been used as an alternative method to improve the physical quality of fish feed, namely stability in water and floatability. Although the fermented fish feed produced had been shown previously to have better stability in water and floatability, the effect of the fermentation on the fish feed nutrition has not yet been known. This study aimed to determine the effect of solid fermentation using the mold Rhizopus sp. on the nutrient content of the fermented feed. In addition, the effect of adding tapioca as much as 0, 1, 2, 3, and 4 g to the dry weight loss and density of the fermented feed was investigated. The results showed that the fermented feed contained higher levels of ash and protein than that before fermentation. The addition of tapioca up to 4 g had no significat effect (p>0.01) on the dry weight loss of the fermented feed, but tended to increase its density compared to those without tapioca addition.Keywords: density, fermentation, fish feed, nutrition, Rhizopus ABSTRAKFermentasi padat menggunakan kapang Rhizopus telah digunakan sebagai metode alternatif untuk memperbaiki kualitas fisik pakan ikan, yakni stabilitas dalam air dan daya apung. Meskipun pakan ikan fermentasi yang dihasilkan sebelumnya sudah dibuktikan memiliki stabilitas dalam air dan daya apung yang lebih baik, namun pengaruh fermentasi terhadap nutrisi pakan ikan tersebut belumlah diketahui. Penelitian ini bertujuan mengetahui pengaruh fermentasi padat menggunakan kapang Rhizopus sp. terhadap kandungan nutrisi pakan fermentasi, dan pengaruh penambahan tapioka sebanyak 0, 1, 2, 3, dan 4 g terhadap kehilangan berat kering dan mass jenis pakan hasil fermentasi. Hasilnya menunjukkan bahwa pakan fermentasi mengandung kadar abu dan protein lebih tinggi dibandingkan sebelum difermentasi. Penambahan tapioka hingga 4 g tidak berpengaruh nyata (p>0,01) pada kehilangan berat kering pakan fermentasi, namun cenderung meningkatkan massa jenis dibandingkan tanpa penambahan tapioka.Kata kunci: fermentasi, massa jenis, nutrisi, pakan ikan, Rhizopus
摘要利用根霉进行固体发酵已成为提高鱼饲料物理质量，即水中稳定性和可浮性的一种替代方法。虽然发酵后的鱼饲料在水中具有较好的稳定性和可浮性，但发酵对鱼饲料营养的影响尚不清楚。本试验旨在研究根霉固体发酵对发酵饲料营养成分的影响。此外，还研究了添加0、1、2、3、4 g木薯粉对发酵饲料干失重和密度的影响。结果表明，发酵后的饲料中灰分和蛋白质含量均高于发酵前。添加4 g木薯粉对发酵饲料的干重损失无显著影响(p>0.01)，但与未添加木薯粉的发酵饲料相比有增加干重的趋势。关键词:密度、发酵、鱼饲料、营养、根霉【关键词】发酵、鱼饲料、营养、根霉【关键词】发酵、鱼饲料、根霉【关键词】发酵、鱼饲料、根霉】我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是，我想说的是。Penelitian ini bertujuan mengetahui pengaruh fermentasi padat menggunakan kapang根霉sp. terhadap kandungan nutrisi pakan fermentasi, dan pengaruh penambahan tapioka sebanyak 0,1,2,3, dan 4 g terhadap kehilangan berat kering dan mass jenis pakan hasil fermentasi。[3]发酵学，发酵学，发酵学，发酵学，发酵学，发酵学，发酵学，发酵学。Penambahan tapioka hingga 4 g tidak berpengaruh nyata (p> 0.01) pada kehilangan berberat kering pakan fermentasi, namun cenderung meningkatkan massa jenis dibandingkan tanpa Penambahan tapioka。Kata kunci:发酵菌，massa jenis，营养菌，pakan ikan，根霉
{"title":"PENGARUH TEKNIK PENGAPUNGAN HAYATI MELALUI FERMENTASI Rhizopus sp. TERHADAP KANDUNGAN NUTRISI PAKAN IKAN APUNG","authors":"S. A. Paramadini, Catur Sriherwanto, Nurlaila, I. Suja’i, M. Annisa","doi":"10.29122/JBBI.V6I1.3477","DOIUrl":"https://doi.org/10.29122/JBBI.V6I1.3477","url":null,"abstract":"Influence of Biofloatation Technique through Rhizopus sp. Fermentation on the Nutritional Quality of the Floating Fish FeedABSTRACTSolid fermentation using the mold Rhizopus has been used as an alternative method to improve the physical quality of fish feed, namely stability in water and floatability. Although the fermented fish feed produced had been shown previously to have better stability in water and floatability, the effect of the fermentation on the fish feed nutrition has not yet been known. This study aimed to determine the effect of solid fermentation using the mold Rhizopus sp. on the nutrient content of the fermented feed. In addition, the effect of adding tapioca as much as 0, 1, 2, 3, and 4 g to the dry weight loss and density of the fermented feed was investigated. The results showed that the fermented feed contained higher levels of ash and protein than that before fermentation. The addition of tapioca up to 4 g had no significat effect (p>0.01) on the dry weight loss of the fermented feed, but tended to increase its density compared to those without tapioca addition.Keywords: density, fermentation, fish feed, nutrition, Rhizopus ABSTRAKFermentasi padat menggunakan kapang Rhizopus telah digunakan sebagai metode alternatif untuk memperbaiki kualitas fisik pakan ikan, yakni stabilitas dalam air dan daya apung. Meskipun pakan ikan fermentasi yang dihasilkan sebelumnya sudah dibuktikan memiliki stabilitas dalam air dan daya apung yang lebih baik, namun pengaruh fermentasi terhadap nutrisi pakan ikan tersebut belumlah diketahui. Penelitian ini bertujuan mengetahui pengaruh fermentasi padat menggunakan kapang Rhizopus sp. terhadap kandungan nutrisi pakan fermentasi, dan pengaruh penambahan tapioka sebanyak 0, 1, 2, 3, dan 4 g terhadap kehilangan berat kering dan mass jenis pakan hasil fermentasi. Hasilnya menunjukkan bahwa pakan fermentasi mengandung kadar abu dan protein lebih tinggi dibandingkan sebelum difermentasi. Penambahan tapioka hingga 4 g tidak berpengaruh nyata (p>0,01) pada kehilangan berat kering pakan fermentasi, namun cenderung meningkatkan massa jenis dibandingkan tanpa penambahan tapioka.Kata kunci: fermentasi, massa jenis, nutrisi, pakan ikan, Rhizopus","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"9 9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116868018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Efrida Martius, Andree Triyadi, D. Y. Sofia, Anis Herliyati Mahsunah
The Effects of Variation in Methanol Concentration and Induction Time on Intracellular Proinsulin Expression by Pichia pastoris ABSTRACTDiabetes is a metabolic disorder characterized by hyperglycemia. There were 215 million diabetic patients in 2014 and the number is expected to rise in 2040. Generally, insulin is used to treat diabetic patients. Insulin production by recombinant technology has been done, though still inefficient, by using E. coli and S. cerevisiae expression system. Another alternative expression system is methylotrophic yeast Pichia pastoris. In this research, proinsulin has been expressed by P. pastoris intracellularly. P. pastoris strains used in this research were X33, GS115, and KM71H. All recombinant strains were MutS. Best cultivation media was BMGY. Proinsulin expression was observed at 25°C. Pichia pastoris strain that expressed proinsulin best was GS115-PI. It was supported by PCR in which the strain GS115-PI gave 504 bp-sized bands. Based on proinsulin formation time, the final methanol concentration of 0.5% in 72 hours was found to be the best treatment.Keywords: BMGY, methanol, phenotype, Pichia pastoris, proinsulin ABSTRAKDiabetes melitus merupakan kelainan yang ditandai dengan hiperglikemia. Penderita diabetes pada tahun 2014 di dunia mencapai 215 juta dan diperkirakan akan meningkat pada tahun 2040. Umumnya penderita diabetes diberi pengobatan insulin sehingga menunjukkan akan ada peningkatan kebutuhan insulin. Produksi insulin dengan teknologi DNA rekombinan telah dilakukan dengan menggunakan sistem ekspresi E. coli dan S. cerevisiae namun masih belum efisien. Sistem alternatif lain adalah ragi metilotropik Pichia pastoris. Dalam penelitian ini dilakukan ekspresi proinsulin dari P. pastoris secara intraseluler. Galur P. pastoris yang digunakan dalam penelitian ini adalah X33, GS115, dan KM71H. Semua galur rekombinan adalah MutS. Media tumbuh terbaik adalah BMGY. Ekspresi proinsulin terlihat pada suhu 25°C. Hasil PCR menunjukkan bahwa galur GS115-PI yang dapat menghasilkan pita amplikon berukuran 504 bp. Hasil PCR ini dibuktikan oleh hasil seleksi galur yang menunjukkan bahwa galur GS115-PI dapat mengekspresi proinsulin dibandingkan galur lainnya. Berdasarkan kecepatan pembentukan pita protein proinsulin, variasi konsentrasi akhir metanol 0,5% dengan lama induksi 72 jam merupakan perlakuan terbaik.Kata Kunci: BMGY, fenotipe, metanol, Pichia pastoris, proinsulin
甲醇浓度和诱导时间对毕赤酵母细胞内胰岛素原表达的影响摘要糖尿病是一种以高血糖为特征的代谢性疾病。2014年有2.15亿糖尿病患者，预计这一数字将在2040年上升。一般来说，胰岛素用于治疗糖尿病患者。利用大肠杆菌和酿酒葡萄球菌的表达系统，利用重组技术生产胰岛素，但效率仍然不高。另一个可选择的表达系统是甲基营养酵母毕赤酵母。在本研究中，胰岛素原蛋白通过酵母在细胞内表达。本研究使用的酵母菌株为X33、GS115和KM71H。所有重组菌株均为MutS。最佳培养基为BMGY。在25℃下观察胰岛素原的表达。表达胰岛素原的毕赤酵母菌株为GS115-PI。PCR结果显示，菌株GS115-PI的条带大小为504个bp。根据胰岛素原的形成时间，在72小时内，最终甲醇浓度为0.5%是最佳处理。关键词:BMGY，甲醇，表型，毕赤酵母，胰岛素原Penderita糖尿病预测，2014年糖尿病预测，2015年糖尿病预测，2015年糖尿病预测，2015年糖尿病预测，2015年糖尿病预测，2040年糖尿病预测。糖尿病糖尿病糖尿病胰岛素胰岛素。胰岛素登革技术、DNA重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组、脱氧核糖核酸重组。系统的替代方案是阿达拉吉鸡和毕赤酵母。胰岛素原蛋白的研究进展。Galur P. pastoris yang digunakan dalam penelitian ini adalah X33, GS115, dan KM71H。Semua galur rekombinan adalah MutS。媒体报道terbaik adalah BMGY。咖啡胰岛素原，胰岛素原，胰岛素原，胰岛素原，胰岛素原，胰岛素原，胰岛素原，胰岛素原。Hasil PCR menunjukkan bahwa galur GS115-PI yang dapat menghasilkan pita amplikon berukuran 504 bp。【关键词】胰岛素原，胰岛素原，胰岛素原，胰岛素原。胰岛素原蛋白，胰岛素原蛋白，胰岛素原蛋白，胰岛素原蛋白，胰岛素原蛋白，胰岛素原蛋白，胰岛素原蛋白，胰岛素原蛋白。Kata Kunci: BMGY, fenotipe，甲醇，毕赤酵母，胰岛素原
{"title":"PENGARUH VARIASI KONSENTRASI METANOL DAN LAMA INDUKSI TERHADAP EKSPRESI PROINSULIN OLEH Pichia pastoris SECARA INTRASELULER","authors":"Efrida Martius, Andree Triyadi, D. Y. Sofia, Anis Herliyati Mahsunah","doi":"10.29122/JBBI.V6I1.3176","DOIUrl":"https://doi.org/10.29122/JBBI.V6I1.3176","url":null,"abstract":"The Effects of Variation in Methanol Concentration and Induction Time on Intracellular Proinsulin Expression by Pichia pastoris ABSTRACTDiabetes is a metabolic disorder characterized by hyperglycemia. There were 215 million diabetic patients in 2014 and the number is expected to rise in 2040. Generally, insulin is used to treat diabetic patients. Insulin production by recombinant technology has been done, though still inefficient, by using E. coli and S. cerevisiae expression system. Another alternative expression system is methylotrophic yeast Pichia pastoris. In this research, proinsulin has been expressed by P. pastoris intracellularly. P. pastoris strains used in this research were X33, GS115, and KM71H. All recombinant strains were MutS. Best cultivation media was BMGY. Proinsulin expression was observed at 25°C. Pichia pastoris strain that expressed proinsulin best was GS115-PI. It was supported by PCR in which the strain GS115-PI gave 504 bp-sized bands. Based on proinsulin formation time, the final methanol concentration of 0.5% in 72 hours was found to be the best treatment.Keywords: BMGY, methanol, phenotype, Pichia pastoris, proinsulin ABSTRAKDiabetes melitus merupakan kelainan yang ditandai dengan hiperglikemia. Penderita diabetes pada tahun 2014 di dunia mencapai 215 juta dan diperkirakan akan meningkat pada tahun 2040. Umumnya penderita diabetes diberi pengobatan insulin sehingga menunjukkan akan ada peningkatan kebutuhan insulin. Produksi insulin dengan teknologi DNA rekombinan telah dilakukan dengan menggunakan sistem ekspresi E. coli dan S. cerevisiae namun masih belum efisien. Sistem alternatif lain adalah ragi metilotropik Pichia pastoris. Dalam penelitian ini dilakukan ekspresi proinsulin dari P. pastoris secara intraseluler. Galur P. pastoris yang digunakan dalam penelitian ini adalah X33, GS115, dan KM71H. Semua galur rekombinan adalah MutS. Media tumbuh terbaik adalah BMGY. Ekspresi proinsulin terlihat pada suhu 25°C. Hasil PCR menunjukkan bahwa galur GS115-PI yang dapat menghasilkan pita amplikon berukuran 504 bp. Hasil PCR ini dibuktikan oleh hasil seleksi galur yang menunjukkan bahwa galur GS115-PI dapat mengekspresi proinsulin dibandingkan galur lainnya. Berdasarkan kecepatan pembentukan pita protein proinsulin, variasi konsentrasi akhir metanol 0,5% dengan lama induksi 72 jam merupakan perlakuan terbaik.Kata Kunci: BMGY, fenotipe, metanol, Pichia pastoris, proinsulin","PeriodicalId":231498,"journal":{"name":"Jurnal Bioteknologi & Biosains Indonesia (JBBI)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114574609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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