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Determination of andrographolide sodium bisulfite in renal microdialysis fluid of rats and toxicokinetic study 大鼠肾微透析液中穿心莲内酯亚硫酸氢钠含量测定及毒性动力学研究
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1776286
Dongming Chen, Jie Chen, Y. Tao, Hong Lu
Abstract Andrographolide sodium bisulfite (ASB) widely used for intestinal infections and respiratory tract infections. It has been reported to frequently cause acute renal failure in clinical practice. The purpose of this study was to evaluate the nephrotoxicity and toxicokinetics in Sprague-Dawley rats after a single-dose injection of ASB administered at 100, 600, and 1000 mg/kg via intravenous tail injections. The concentrations in plasma and kidney microdialysates were analyzed by high-performance liquid chromatography. Blood urea nitrogen and creatinine levels in plasma were determined and toxicokinetic parameters were observed. In plasma and kidney, the elimination constant and clearance were decreased and the half-time was increased with increasing dose. Blood urea nitrogen and creatinine were positively correlated with ASB concentration indicating the potential for accumulation in kidney that can eventually lead to damage.
摘要穿心莲内酯亚硫酸氢钠(ASB)广泛用于肠道感染和呼吸道感染。据报道,在临床实践中,它经常引起急性肾功能衰竭。本研究的目的是评估Sprague-Dawley大鼠经尾静脉注射单剂量ASB (100mg /kg、600mg /kg和1000mg /kg)后的肾毒性和毒性动力学。采用高效液相色谱法分析血浆和肾脏微透析液中的浓度。测定血浆尿素氮、肌酐水平,观察毒化动力学参数。在血浆和肾脏中,随着剂量的增加,消除常数和清除率降低,半衰期延长。血尿素氮和肌酐与ASB浓度呈正相关,表明ASB可能在肾脏积聚,最终导致损害。
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引用次数: 1
Mitochondrial toxicity and in vitro biological effects of ambient PM2.5 from an urban site in China 中国城市环境PM2.5的线粒体毒性及体外生物学效应
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1767780
Zhi-Jing Lin, Jinzhuo Li, Qianwen Zeng, R. Sun
Abstract The roles of PM2.5-induced mitochondrial damage and oxidative stress on mast cell degranulation were examined in vitro. Mast cells were treated with suspensions of PM2.5 in Dulbecco’s modified Eagle’s medium at concentrations from 25 to 200 mg/L in the absence or presence of 10 mmol/L N-acetyl-L-cysteine. Biological effects and mitochondrial function were assessed by determining cell viability, β-hexosaminidase release, interleukin-4 secretion, reactive oxygen species generation, adenosine triphosphate production, potential alteration of mitochondrial membrane, and activities of mitochondrial electron transport chain complexes I and III. Exposure of mast cells to PM2.5 induced reduction of adenosine triphosphate production, collapse of mitochondrial membrane potential, and inhibition of the activity of complex III. Co-treatment of mast cells exposed to PM2.5 with N-acetyl-L-cysteine attenuated cytotoxicity and the production of reactive oxygen species, and decreased the release of β-hexosaminidase and interleukin-4. Evidently, PM2.5-induced oxidative stress plays an essential role in mitochondrial toxicity and mast cell activation.
研究了pm2.5诱导的线粒体损伤和氧化应激对肥大细胞脱颗粒的作用。在不含或存在10 mmol/L n -乙酰-L-半胱氨酸的情况下,在Dulbecco改良Eagle培养基中,用浓度为25至200 mg/L的PM2.5混悬液处理肥大细胞。通过测定细胞活力、β-己糖氨酸酶释放、白细胞介素-4分泌、活性氧生成、三磷酸腺苷生成、线粒体膜的潜在改变以及线粒体电子传递链复合物I和III的活性来评估生物效应和线粒体功能。肥大细胞暴露于PM2.5中会导致三磷酸腺苷生成减少,线粒体膜电位崩溃,复合物III活性抑制。暴露于PM2.5的肥大细胞与n-乙酰- l-半胱氨酸共同处理可降低细胞毒性和活性氧的产生,并减少β-己糖氨酸酶和白细胞介素-4的释放。显然,pm2.5诱导的氧化应激在线粒体毒性和肥大细胞活化中起重要作用。
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引用次数: 0
Health risk assessment of trace elements through exposure of particulate matter-10 during the cooking of Ethiopian traditional dish sauces 埃塞俄比亚传统菜肴酱料烹饪过程中接触颗粒物质-10对微量元素的健康风险评估
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1770257
A. Embiale, B. Chandravanshi, F. Zewge, E. Sahle-Demessie
Abstract This study was aimed to analyze trace elements in the particulate matter-10 and evaluate their health risks during the cooking of the most widely consumed Ethiopian traditional dish sauces (Wots) using charcoal, kerosene and electricity stoves. The trace elements (iron, cadmium, arsenic, chromium, lead, boron, nickel, cobalt, tin, copper and zinc) in the particulate matter-10 were found in the range 0.001–0.175 µg m−3. The human health risk assessment has done based on the United States Environmental Protection Agency prescription. The hazard quotient and hazard index values using charcoal, kerosene and electricity stoves were found below 1. This result showed that the inhabitants stay at any of these three microenvironments has no likelihood to have non-cancer health problems. In addition, the life time cancer values for all trace elements were below the tolerable range set by United States Environmental Protection Agency, except chromium, cadmium and arsenic which were found within the tolerable range. Furthermore, the total sum of eleven determined elements was calculated, and the highest concentration was observed using kerosene stove followed by charcoal and electricity stoves, respectively. The use of kerosene and charcoal stove were not the recommended stove as compared to electricity stove for the cooking of Wot.
本研究旨在分析使用木炭、煤油和电炉烹饪最广泛使用的埃塞俄比亚传统菜肴酱汁(wot)时颗粒物-10中的微量元素,并评估其健康风险。颗粒物-10中微量元素(铁、镉、砷、铬、铅、硼、镍、钴、锡、铜和锌)含量在0.001 ~ 0.175µg m−3之间。人类健康风险评估是根据美国环境保护署的处方进行的。使用木炭炉、煤油炉和电炉的危害商值和危害指数均小于1。这一结果表明,居住在这三种微环境中的居民不可能出现非癌症健康问题。此外,除铬、镉和砷在可耐受范围内外,所有微量元素的终生致癌值均低于美国环境保护局规定的可耐受范围。计算了11种元素的总和,煤油炉的浓度最高,其次是木炭炉和电炉。与电炉相比,使用煤油炉和木炭炉是不推荐的灶具。
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引用次数: 8
Transfer rate of pesticide residues from medicinal plants in different types of extractive solutions 药用植物中农药残留在不同类型萃取液中的转移率
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1773466
A. Florea, V. Drumea, R. Nita, A. Bicu, L. Olariu, L. E. Duțu, C. Gîrd
Abstract Starting from the suspicion that the medicinal herbs may contain traces of pesticides and taking into account the risks of patients being exposed to contaminated products, the aim of this research was to evaluate the pesticide residues and the degree of their transfer (%) in three types of preparations (infusion, decoctionand cold maceration), for four medicinal plant species very often used in phytotherapy (Rosmarini folium, Menthae folium, Saturejae herba and Basilica herba). For each type of plant product, four samples were purchased from different manufacturers and they have been analyzed using gas chromatography- tandem mass spectrometry. One out of four samples of the same plant species contain at least one pesticide residue above the maximum level and shows a significant transfer of these residues in aqueous extractive solutions during extraction. The highest rate of pesticide transfer from medicinal plants was identified in infusions, recommended by many manufacturers.
摘要:本研究从怀疑中药中可能含有微量农药入手,考虑到患者接触污染产品的风险,对中药中常用的四种药用植物(迷迭香叶、薄荷叶、红花叶和Basilica herba)的三种制剂(冲剂、煎剂和冷浸剂)的农药残留及其转移程度(%)进行了评价。对于每种类型的植物产品,从不同的制造商购买了四个样品,并使用气相色谱-串联质谱法进行了分析。同一植物种类的四分之一样品中含有至少一种高于最高水平的农药残留,并且在提取过程中这些残留物在水萃取溶液中显著转移。农药从药用植物转移率最高的是许多制造商推荐的注射剂。
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引用次数: 1
Carbon black-benzo(a)pyrene complex-induced apoptosis and autophagy blockage in rat alveolar macrophages 炭黑-苯并芘复合物诱导大鼠肺泡巨噬细胞凋亡和自噬阻断
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1766044
Zhuoqun Meng, Yixuan Huang, Yan Chen, Siyu Tao, Meitong Liu, Jing Lu, S. Guan
Abstract Cooking of foods and the burning of biomass and fossil fuels in stoves are the main sources of cooking fumes, with carbon black and polycyclic aromatic hydrocarbons as main components. The toxicity of carbon black and polycyclic aromatic hydrocarbons has been well studied individually, but the combined toxicity is much less understood. Carbon black can adsorb benzo(a)pyrene to form a complex which displays an altered physical form which in this study has been constructed to simulate particles in the cooking fumes and explore the combined toxic effect on rat alveolar macrophages. The complex-induced cell apoptosis and blocked cell autophagy flux compared with both individually. The mechanism of toxicity may be by intracellular reactive oxygen species generation, impairing the mitochondrial membrane potential and activating apoptosis signaling pathways. Graphical Abstract
烹饪食物以及在炉子中燃烧生物质和化石燃料是烹饪油烟的主要来源,其中炭黑和多环芳烃是主要成分。炭黑和多环芳烃的毒性已经分别得到了很好的研究,但其联合毒性却知之甚少。炭黑可以吸附苯并(a)芘形成一种复合物,这种复合物表现出一种改变的物理形态,本研究构建了这种复合物来模拟烹饪烟雾中的颗粒,并探索其对大鼠肺泡巨噬细胞的综合毒性作用。复合物诱导细胞凋亡和阻断细胞自噬通量与两者单独比较。其毒性机制可能与细胞内活性氧生成、线粒体膜电位受损和细胞凋亡信号通路激活有关。图形抽象
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引用次数: 2
Fungicidal activity and molecular modeling of fusarubin analogues from Fusarium oxysporum 尖孢镰刀菌红素类似物的杀真菌活性及分子模拟
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1770253
A. Kundu, A. Mandal, S. Saha, P. Prabhakaran, S. Walia
Abstract Fusarubin analogues of Fusarium oxysporum f. sp. ciceris were investigated for antifungal activity in vitro against five soil borne phytopathogenic fungi. 3-O-Methyl-8-O-methyl-fusarubin was inhibitory towards S. sclerotiorum (EC50 0.33 mmol L−1) and Sclerotium rolfsii (EC50 0.38 mmol L−1). A structure–antifungal activity relationship of fusarubin analogues was established from their activity performance. Possible mechanism of action of these compounds was studied using molecular docking and simulations against three target enzymes which revealed receptor ligand binding affinity. Docking of 3-O-methyl-8-O-methyl-fusarubin into the succinate dehydrogenase site revealed formation of salt bridge, hydrogen bond, π–anion, π–alkyl, and Van der Waals interactions.
摘要研究了镰刀菌(Fusarium oxysporum f. sp. ciceris)镰刀菌素类似物对5种土传植物病原真菌的体外抑菌活性。3- o -甲基-8- o -甲基-fusarubin对菌核菌(S. sclerotiorum, EC50为0.33 mmol L−1)和罗氏菌核菌(Sclerotium rolfsii, EC50为0.38 mmol L−1)有抑制作用。从活性表现出发,建立了赤霉素类似物的结构-抗真菌活性关系。通过对三种靶酶的分子对接和模拟,研究了这些化合物可能的作用机制,揭示了受体配体的结合亲和力。3- o -甲基-8- o -甲基-赤霉素与琥珀酸脱氢酶位点对接,发现盐桥、氢键、π -阴离子、π -烷基和范德华相互作用的形成。
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引用次数: 5
Larvicidal, repellent, and histopathologic effects of Citrullus colocynthis against the malaria vector 瓜泥对疟疾病媒的杀幼虫、驱避和组织病理学作用
Pub Date : 2020-04-20 DOI: 10.1080/02772248.2020.1770254
Mahsa Akbarzadeh, Aboozar Soltani, M. Moemenbellah-Fard, M. Khoshnoud, K. Azizi
Abstract This research is conducted to determine the larvicidal, repellent and pathological effects of fruit extracts of C. colocynthis on malaria vector mosquito, Anopheles stephensi Liston, 1901. The fruit water and ethanol extract of C. colocynthis were concentrated for larvicidal and repellent activities on human volunteer against An. stephensi. The temephos larvicide was tested as the positive control. A concentration of 300 mg/L had full-scale mortality and the most toxic effect. The LC50 and LC90 values of C. colocynthis water and ethanol extracts were 180and 283 mg/L, respectively. The LC50 and LC90 values of temephos were 0.2706 and 1.6932 mg/L, respectively. The ED50 and ED90 values of the plant extract were 0.2 and 1.3 mg/cm2, respectively. Thin sections from treated larvae exhibited gross histopathologic effects of C. colocynthis fruit extracts on gut epithelial cell layer by vacuolization of foregut cuboidal and midgut columnar cells. This study suggested that the fruit extracts of C. colocynthis had significant larvicidal and repellent activity to be used as an ideal approach to control the malaria vector mosquito.
摘要本研究旨在研究紫锥虫果实提取物对疟疾病媒斯氏按蚊(Anopheles stephensi Liston, 1901)的杀幼虫、驱避及病理作用。对其果水和乙醇提取物进行浓缩,测定其对人体志愿者的杀虫和驱避作用。stephensi。以杀幼虫双硫磷为阳性对照。浓度为300 mg/L具有完全死亡率和最大的毒性作用。水提取物和乙醇提取物的LC50和LC90值分别为180和283 mg/L。双硫磷的LC50和LC90分别为0.2706和1.6932 mg/L。植物提取物的ED50和ED90值分别为0.2和1.3 mg/cm2。从处理过的幼虫的薄片上可以看出,巨藻果提取物对肠道上皮细胞层的影响主要表现为前肠立方体细胞和中肠柱状细胞的空泡化。结果表明,该果实提取物具有显著的杀幼虫和驱避活性,可作为防治疟疾病媒蚊虫的理想方法。
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引用次数: 5
Toxicity of a phenoxy herbicide on the lichen Ramalina fraxinea 一种苯氧类除草剂对凤尾苔的毒性研究
Pub Date : 2019-11-26 DOI: 10.1080/02772248.2020.1747466
G. Sujetovienė, Kotryna Gasauskaitė, J. Žaltauskaitė
2-Methyl-4-chlorophenoxyacetic acid has been widely used as herbicide in the United States and Europe. This study aims to quantify its effects at concentrations of 0–100 mg/L for up to 48 h on the ...
2-甲基-4-氯苯氧乙酸在美国和欧洲被广泛用作除草剂。本研究旨在量化其在0-100 mg/L浓度下长达48小时对…
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引用次数: 4
Characteristics of fume sedimentation in the working zone during arc welding with covered electrodes 覆盖电极弧焊工作区内烟尘沉降特性
Pub Date : 2019-11-26 DOI: 10.1080/02772248.2020.1747465
K. Kirichenko, I. Vakhniuk, R. Rogulin, A. V. Kirichenko, A. Gridasov, D. Kosyanov, V. Drozd, A. S. Kholodov, W. Piekoszewski, K. Golokhvast
In order to develop effective protection measures and reduce the number of diseases in the welding industry, a detailed study of the mechanism of propagation of welding fumes in the working zone is...
为了制定有效的防护措施,减少焊接行业的疾病发生,本文对焊接烟气在工作区域的传播机理进行了详细的研究。
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引用次数: 1
The effect of uranium, radium and thorium speciation on the removal of radioactivity from acid mine drainage using ion exchange 离子交换法去除酸性矿山废水放射性的铀、镭、钍形态的影响
Pub Date : 2019-11-26 DOI: 10.1080/02772248.2020.1747464
Thulani C. Dlamini, V. Tshivhase, P. Maleka
Acid mine drainage samples were collected from the central basin of the Witwatersrand basin in South Africa to study the removal of radioactivity. The samples were analyzed for gross alpha/beta act...
从南非威特沃特斯兰德盆地的中央盆地收集了酸性矿井排水样本,以研究放射性的去除。对样品进行了总α / β行为分析。
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引用次数: 0
期刊
Toxicological & Environmental Chemistry
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