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Parity affects mammary development in late-pregnant swine 胎次影响妊娠后期猪的乳腺发育
IF 1.3 Q2 Veterinary Pub Date : 2024-03-23 DOI: 10.1093/tas/txae037
Chantal Farmer, Jakob C Johannsen, Caroline Gillies, L. Huber, R. C. Hovey
The goal of this project was to determine if various measures of mammary development differed between gilts and multiparous sows at the end of gestation. During gestation, Yorkshire × Landrace gilts (n=19) and sows (second and third gestations, n = 17) were fed one daily meal of a conventional corn-based diet, where amount fed was based on body weight (BW) and backfat thickness (BF) at mating. On day 110 ± 1 of gestation, a jugular blood sample was obtained from all gilts and sows to measure insulin-like growth factor-1 (IGF-1), glucose, free fatty acids (FFA) and urea. On that same day BW and BF were measured and animals were euthanized. Mammary glands from one side of the udder were dissected for compositional analyses. The fifth gland of the contralateral row of mammary glands was sampled for histology and immunohistochemical localization of Ki67. There was less total parenchyma (1437.4 vs 2004.7 ± 127.1 g; P < 0.001) and total extraparenchymal tissue (1691.0 vs 2407.0 ± 125.3 g; P < 0.001) in mammary glands of gilts compared to those from sows. When these values were expressed per kg BW (226.0 and 284.0 ± 2.7 kg for gilts and sows, respectively), parenchymal mass did not differ (P > 0.10), while extraparenchymal tissue weight tended to be less in gilts than sows (P = 0.07). All components within the parenchyma differed by parity (P < 0.001). Specifically, parenchymal tissue from gilts contained a greater proportion of fat and dry matter (DM), a lower proportion of protein, and lower concentrations of DNA (6.59 vs 9.35 ± 0.53 mg/g DM) and RNA (7.76 vs 12.33 ± 0.70 mg/g DM) than that from sows. On the other hand, the circumference of alveolar lumens was greater in gilts than sows (P < 0.001), while the percentage of epithelial cells that were positive for Ki67, a marker of cell proliferation, was greater in sows than gilts (P < 0.05). Circulating concentrations of IGF-1 were greater in gilts than multiparous sows (45.0 vs 27.3 ± 2.8 ng/mL, P < 0.001). None of the other blood variables were changed by parity. Results show a marked effect of parity on mammary gland development in swine. At the end of gestation, the mammary glands of gilts had less parenchyma with lower epithelial proliferation than glands from multiparous sows. These differences could alter the response of mammary tissue to various nutritional or endocrine signals. This information is crucial for the development of management strategies designed to maximize sow milk yield.
本项目旨在确定后备母猪和多胎母猪在妊娠末期乳腺发育的各种指标是否存在差异。在妊娠期间,约克夏×兰德良种后备母猪(n=19)和母猪(第二胎和第三胎,n=17)每天饲喂一餐常规玉米日粮,饲喂量基于交配时的体重(BW)和背膘厚度(BF)。在妊娠期第 110 ± 1 天,从所有后备母猪和母猪身上采集颈静脉血样,以测定胰岛素样生长因子-1 (IGF-1)、葡萄糖、游离脂肪酸 (FFA) 和尿素。同一天测量体重和体脂,然后对动物实施安乐术。解剖一侧乳房的乳腺进行成分分析。对侧乳腺排的第五个腺体取样进行组织学和 Ki67 免疫组化定位。与母猪相比,后备母猪乳腺的实质组织总量(1437.4 vs 2004.7 ± 127.1 g;P < 0.001)和实质外组织总量(1691.0 vs 2407.0 ± 125.3 g;P < 0.001)较少。当这些值以每公斤体重表示时(后备母猪和母猪分别为 226.0 公斤和 284.0 ± 2.7 公斤),实质质量没有差异(P > 0.10),而后备母猪的乳腺实质外组织重量往往低于母猪(P = 0.07)。实质内的所有成分都因母猪的雌雄而异(P < 0.001)。具体来说,与母猪相比,后备母猪的实质组织中脂肪和干物质(DM)的比例更高,蛋白质的比例更低,DNA(6.59 vs 9.35 ± 0.53 mg/g DM)和RNA(7.76 vs 12.33 ± 0.70 mg/g DM)的浓度更低。另一方面,后备母猪的肺泡腔周长大于母猪(P < 0.001),而细胞增殖标记物 Ki67 阳性的上皮细胞百分比,母猪高于后备母猪(P < 0.05)。后备母猪的 IGF-1 循环浓度高于多胎母猪(45.0 vs 27.3 ± 2.8 ng/mL,P < 0.001)。其他血液变量均不因胎次而改变。结果表明,猪的奇数对乳腺发育有明显影响。在妊娠末期,后备母猪乳腺的实质较少,上皮增生程度低于多胎母猪的乳腺。这些差异可能会改变乳腺组织对各种营养或内分泌信号的反应。这些信息对于制定旨在最大限度提高母猪产奶量的管理策略至关重要。
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引用次数: 0
Employee perception of precision technology use at the dairy farm 员工对奶牛场使用精准技术的看法
IF 1.3 Q2 Veterinary Pub Date : 2024-03-22 DOI: 10.1093/tas/txae036
Natalia Herrera, Juan Vélez, Timothy Holt, Pablo J Pinedo
The adoption of precision technologies on dairy farms has increased significantly in recent decades, leading to the challenge of providing employees with resources to maximize the efficient use of these tools. The objective of this study was to explore how dairy farm employees perceive the available precision technologies and to identify possible challenges they face when adapting to their use at the farm. An online survey consisting of four sections (employee demographics, precision technologies in use, perception of these technologies, and opportunities for adapting to technology use) was completed from September to December 2022 by 266 farm employees from three dairies operated under similar management. Most of the respondents were identified as male (72.2%), Hispanic or Latino (92.5%), ages between 21 to 30 (39.1%) or 31 to 40 years old (36.8%), with a bachelor’s degree (34.6%) or completion of middle school (29.3%) and having basic or no English proficiency (74%). Overall, the respondents indicated being comfortable (95.6%) with and understanding (91.8%) the technology they use. Employees recognized precision technology as a tool that helps them to be more efficient (93.7%), identifying the technologies’ benefits (92.1%). However, challenges for adapting to these technologies included personal limitations, such as not knowing the language of the technology (31%), visual impairments (24%), light sensitivity (14%), and not being able to read (7%). Environmental limitations were also recognized and included cold weather (64.3%), wind (46%), and surroundings that are too dark (31%) or too bright (21%). Significant associations between perception of the technology and age, level of education, and English proficiency were identified. Respondents indicated their desire to learn more about precision technologies implemented at work, which could eventually lead to improved efficiency at the dairy operation through innovations in the way users interact with these technologies, increasing employees’ motivation. This study provides insights that could assist the dairy industry in addressing challenges and enhancing opportunities for a more efficient use of precision technologies at dairy farms.
近几十年来,奶牛场对精准技术的采用大幅增加,这给为员工提供资源以最大限度地高效使用这些工具带来了挑战。本研究旨在探讨奶牛场员工如何看待现有的精准技术,并确定他们在适应使用这些技术时可能面临的挑战。2022 年 9 月至 12 月,来自三个管理类似的奶牛场的 266 名牧场员工完成了一项在线调查,调查内容包括四个部分(员工人口统计、使用中的精准技术、对这些技术的看法以及适应技术使用的机会)。大多数受访者被认定为男性(72.2%)、西班牙裔或拉丁裔(92.5%)、年龄介于 21 至 30 岁(39.1%)或 31 至 40 岁(36.8%)之间、拥有学士学位(34.6%)或完成初中学业(29.3%)、具备基本或零英语水平(74%)。总体而言,受访者表示对所使用的技术感到舒适(95.6%)并理解(91.8%)。员工认为精密技术是帮助他们提高工作效率的工具(93.7%),并指出了技术的好处(92.1%)。然而,适应这些技术所面临的挑战包括个人限制,如不懂技术语言(31%)、视力障碍(24%)、对光线敏感(14%)和不识字(7%)。环境限制也得到了认可,包括寒冷的天气(64.3%)、大风(46%)、周围环境太暗(31%)或太亮(21%)。受访者对该技术的看法与年龄、教育水平和英语水平之间存在显著关联。受访者表示,他们希望更多地了解在工作中应用的精准技术,通过创新用户与这些技术的互动方式,最终提高奶牛场的运营效率,从而提高员工的积极性。本研究提供的见解可帮助乳制品行业应对挑战,并增加在奶牛场更有效地使用精准技术的机会。
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引用次数: 0
Replacing alfalfa hay with a novel alfalfa leaf pellet product (ProLEAF MAX) and/or alfalfa stems (ProFiber Plus) in the diet of developing dairy heifers alters dry matter intake, but does not negatively impact growth or development 用新型苜蓿叶颗粒产品(ProLEAF MAX)和/或苜蓿茎(ProFiber Plus)代替苜蓿干草喂养发育中的乳用小母牛会改变干物质摄入量,但不会对生长或发育产生负面影响
IF 1.3 Q2 Veterinary Pub Date : 2024-03-19 DOI: 10.1093/tas/txae038
L. A. Motsinger, A. Young, R. Feuz, R. Larsen, T. J. Brady, R. K. Briggs, C. Reichhardt, C. Pratt, K. J. Thornton
Alfalfa is a commonly grown forage in the Intermountain West region of the US and is often included in the diet of dairy cattle. Alfalfa provides a variety of different nutrients, but the nutrient content of alfalfa varies depending on factors such as the soil, region, cutting, and climate. However, alfalfa leaves tend to have less variation in their nutrient content than alfalfa stems. Fractionating alfalfa may be one way to improve control of nutrients provided when developing a ration for developing dairy heifers. The purpose of this study was to determine whether including fractionated alfalfa in the diet impacts growth or conception rates of developing dairy heifers. Heifers were allocated to one of three treatments: a control group fed a typical diet (CON; n=8), a diet that replaced alfalfa with fractionated alfalfa leaf pellets and alfalfa stems (ProLEAF MAX + ProFiber Plus; PLM+PFP; n=8), or a diet that replaced alfalfa with alfalfa stems (PFP; n=8) for 85 d. Heifers were fed individually twice daily and weight, hip height, and wither height were recorded every 14 d. Additionally, blood was collected every 28 d and conception rates were recorded at the end of the trial. Heifers receiving the PFP diet consumed less dry matter (P = 0.001) than the CON treatment. Analyses were then conducted to determine nutrient intake and heifers receiving the PFP diet also consumed less neutral detergent fiber (P = 0.017), acid detergent fiber (P = 0.023), crude protein (P = 0.001), and net energy for maintenance (P = 0.001) than heifers consuming the CON diet; however, no differences (P > 0.10) were observed between heifers fed the CON and PLM+PFP diets. Analysis of body weight gain over the feeding period showed no difference (P = 0.52) among heifers consuming the different treatment diets (Table 3). Additionally, treatment did not affect average daily gain (P = 0.49), gain:feed (P = 0.82), hip height gain (P = 0.20) or wither height gain (P = 0.44) among heifers receiving different diets. Treatment ×time altered (P < 0.001) BUN when analyzed as a repeated measure. Total feed cost was lowest (P < 0.001) for the PFP diet and cost of gain tended (P = 0.09) to be increased for the PLM+PFP diet compared to the CON diet. Overall, these data indicate that including alfalfa stems in a developing heifer diet may decrease dry matter intake, lower input costs and increase profitability, without negatively impacting growth.
紫花苜蓿是美国中西部地区普遍种植的牧草,通常被纳入奶牛的饲料中。紫花苜蓿可提供多种不同的营养成分,但紫花苜蓿的营养成分因土壤、地区、切割和气候等因素而异。不过,与苜蓿茎相比,苜蓿叶的营养成分变化往往较小。在为发育中的乳用小母牛配制饲料时,将紫花苜蓿细分可能是改善营养成分控制的一种方法。本研究的目的是确定在日粮中添加碎苜蓿是否会影响发育中的乳用小母牛的生长或受胎率。母牛被分配到三种处理中的一种:对照组,饲喂普通日粮(CON;n=8);用分馏苜蓿叶颗粒和苜蓿茎替代苜蓿的日粮(ProLEAF MAX + ProFiber Plus;PLM+PFP;n=8);或用苜蓿茎替代苜蓿的日粮(PFP;n=8),饲喂 85 天。此外,每隔 28 天采集一次血液,试验结束时记录受孕率。与 CON 处理相比,接受 PFP 日粮的母牛消耗的干物质较少(P = 0.001)。然后进行分析以确定养分摄入量,与饲喂 CON 日粮的小母牛相比,饲喂 PFP 日粮的小母牛消耗的中性洗涤纤维(P = 0.017)、酸性洗涤纤维(P = 0.023)、粗蛋白(P = 0.001)和维持所需的净能(P = 0.001)也较少;但是,饲喂 CON 日粮和 PLM+PFP 日粮的小母牛之间未观察到差异(P > 0.10)。对饲喂期间体重增加的分析表明,饲喂不同处理日粮的母牛之间没有差异(P = 0.52)(表 3)。此外,处理对不同日粮的小母牛的平均日增重(P = 0.49)、增重:饲料(P = 0.82)、臀高(P = 0.20)或枯萎高度(P = 0.44)均无影响。在进行重复测量分析时,处理 × 时间会改变尿素氮(P < 0.001)。PFP日粮的饲料总成本最低(P < 0.001),与CON日粮相比,PLM+PFP日粮的增重成本有增加的趋势(P = 0.09)。总之,这些数据表明,在发育中的小母牛日粮中添加紫花苜蓿茎可减少干物质摄入量、降低投入成本并提高收益率,而不会对生长产生负面影响。
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引用次数: 0
Effect of creep feeding pelleted starter diet, liquid milk replacer and a liquid mixture of starter diet and milk replacer to suckling pigs on their growth and medication usage 用颗粒初乳日粮、液态代乳粉以及初乳日粮和代乳粉的液态混合物喂养乳猪对其生长和用药的影响
IF 1.3 Q2 Veterinary Pub Date : 2024-03-16 DOI: 10.1093/tas/txae041
E. Arnaud, G. E. Gardiner, M. Chombart, J. V. O Doherty, T. Sweeney, P. G. Lawlor
The aim of this study was to assess the effect of creep feeding solid starter diet, liquid milk replacer and a liquid mixture of starter diet and milk replacer to suckling pigs on their growth and medication usage up to target slaughter weight (~120 kg). Ninety-one sows and their litters were randomly assigned to one of four post-farrowing treatments at day 107 of gestation; 1) no creep feed provided to weaning at day 28 of age (CONTROL; n=20), 2) dry pelleted starter diet provided as creep feed from day 10 of age to weaning (DPS; n=25), 3) liquid milk replacer provided as creep feed from day 3 of age to weaning (LMR; n=23), and 4) liquid milk replacer provided from day 3 to 6 of age followed by a mixture of liquid milk replacer with an increasing proportion of liquid starter diet to weaning provided as creep feed (LMR+S; n=23). Pig weight and dry matter disappearance (DMd) were recorded during lactation and post-weaning until pigs reached target slaughter weight (~120 kg). At target slaughter weight, carcass weight and quality were recorded. Medication (antibiotic and anti-inflammatory) usage per pig on a litter basis, and number of injections and clinical cases of disease per litter were recorded from birth to slaughter. At day 5 post-weaning, a subset of pigs (n=40) were sacrificed and intestinal samples were collected for histological analysis. Piglets supplemented with DPS had higher DMd of creep feed than those supplemented with LMR or LMR+S (P<0.001). Providing LMR+S to suckling piglets reduced the coefficient of variation (CV) for within-litter piglet weaning weight (P<0.01) compared to DPS and LMR, but CV of LMR+S was similar to that of CONTROL. Providing DPS or LMR to suckling piglets increased piglet weaning weight compared to CONTROL (P<0.001) but pig weight was not significantly different from CONTROL at time-points thereafter. Gain to feed ratio from weaning to day 6 post-weaning was less for LMR pigs compared to all other treatments (P<0.001). Providing DPS or LMR+S to suckling piglets tended to increase post-weaning ileal villus height (P=0.07). Diarrhoea incidence, as well as the number of clinical cases of disease and injections per litter and volume of antibiotic and anti-inflammatory administered per pig pre- and post-weaning were not affected by treatment (P>0.05). In conclusion, supplementing suckling pigs with liquid milk replacer or dry pelleted starter improved growth at weaning, but the benefit did not persist to slaughter.
本研究的目的是评估乳猪匍匐饲喂固体开食料、液态代乳粉以及开食料和代乳粉的液态混合物对其生长和用药直至目标屠宰体重(约 120 千克)的影响。在妊娠第 107 天,将 91 头母猪及其仔猪随机分配到四种出栏后处理中的一种:1)不提供爬行饲料至 28 日龄断奶(CONTROL;n=20);2)从第 10 日龄至断奶提供干颗粒开食料作为爬行饲料(DPS;n=25);3)从第 3 日龄至断奶期间提供液态代乳品作为爬行饲料(LMR;n=23);4)从第 3 日龄至第 6 日龄提供液态代乳品,然后混合液态代乳品和比例不断增加的液态初乳日粮作为爬行饲料提供至断奶(LMR+S;n=23)。在哺乳期和断奶后记录猪的体重和干物质消失量(DMd),直到猪达到目标屠宰体重(约 120 千克)。在目标屠宰体重时,记录胴体重量和质量。记录每窝猪从出生到屠宰期间的用药情况(抗生素和消炎药)、注射次数和临床病例。断奶后第 5 天,一部分猪(n=40)被宰杀,并采集肠道样本进行组织学分析。与添加 LMR 或 LMR+S 的仔猪相比,添加 DPS 的仔猪的爬行饲料 DMd 更高(P0.05)。总之,给乳猪补充液态代乳粉或干颗粒开食料可提高断奶时的生长速度,但这种益处不会持续到屠宰。
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引用次数: 0
Data Analytics-based Evaluation of Blood Indices and adaptation of medicated and non-medicated Broiler Chickens under Humid Tropical Conditions 基于数据分析的血液指标评估以及用药和不用药肉鸡在潮湿热带条件下的适应性研究
IF 1.3 Q2 Veterinary Pub Date : 2024-03-16 DOI: 10.1093/tas/txae040
O. M. Alabi, H. O. Aworinde, S. Adebayo, A. O. Akinwumi, A. Ayandiji, A. Tarta
The growth performance and blood indices of medicated and non-medicated broiler chickens have been the subject of this research couple with a paucity of comparative information on what can actually happen to broiler chickens if not medicated when reared under humid tropical conditions. One hundred unsexed day-old broilers were randomly and equally allotted into two treatment groups of TM (medicated) and TN (non-medicated) in a completely randomized design (CRD) each treatment with five replicates having ten birds each. The birds were reared on deep litter system for 56 days which was divided into two phases of 28 days each (starter and finisher), during which data were collected with respect to daily feed intake (DFI), final body weight (FBW), body weight gained (BWG), mortality rate while blood analysis was carried out on 28th and 56th day for starter and finisher phases respectively. Non-medicated group served as control. Feed conversion ratio (FCR) and Feed conversion efficiency (FCE), were later calculated. Data collected were subjected to analysis of variance statistically.There was no significant difference between the medicated and non-medicated broilers for DFI, FBW and BWG and also for the blood parameters investigated at starter phase. However, at finisher phase, no significant difference was observed in the DFI of Tm and Tn but there was significant (p<0.05) difference in the FBW, BWG, FCR, FCE, and mortality rate between the two groups. Birds on Tm attained higher weight significantly (p<0.05) than those on TN. BWG, FCR, FCE followed the same trend and also the mortality rate. White blood cells count of TN was higher significantly (p<0.05) than TM while TM birds recorded higher packed cell volume (PCV), red blood counts (RBC) and haemoglobin concentration (Hb) significantly (p<0.05) than TM birds. Effect of medication was much noticeable at finisher phase as it improved the growth rate though mortality rate was close to that of TN group. These results suggest that broilers can be produced free of medication with good feeding without loss of birds while the growth rate can be enhanced with the use of prebiotics and prebiotics.
在热带潮湿条件下饲养肉鸡时,如果不使用药物,肉鸡的生长性能和血液指数究竟会如何,这方面的比较信息很少,因此,使用药物和不使用药物的肉鸡的生长性能和血液指数一直是本研究的主题。在完全随机设计(CRD)中,100 只未受精的日龄肉鸡被随机平均分配到两个处理组:TM(用药)组和 TN(不用药)组,每个处理组有五个重复,每个重复有 10 只鸡。在此期间,收集每日采食量 (DFI)、最终体重 (FBW)、增重 (BWG) 和死亡率方面的数据,并分别在第 28 天和第 56 天对初养鸡和终养鸡阶段进行血液分析。未用药组作为对照组。随后计算饲料转化率(FCR)和饲料转化效率(FCE)。对收集到的数据进行了统计方差分析。在开产阶段,用药组和未用药组的肉鸡在DFI、FBW和BWG以及血液参数方面没有显著差异。然而,在育成期,Tm 和 Tn 的 DFI 没有观察到显著差异,但两组之间的 FBW、BWG、FCR、FCE 和死亡率有显著差异(p<0.05)。服用 Tm 的鸟类的体重明显高于服用 TN 的鸟类(p<0.05)。BWG、FCR、FCE和死亡率也呈相同趋势。TN 禽的白细胞计数明显高于 TM 禽(p<0.05),而 TM 禽的包装细胞体积(PCV)、红细胞计数(RBC)和血红蛋白浓度(Hb)明显高于 TM 禽(p<0.05)。药物治疗在育成期的效果非常明显,因为它提高了生长速度,尽管死亡率与 TN 组接近。这些结果表明,肉鸡可以在不使用药物的情况下通过良好的饲养而不造成损失,同时使用益生素和益生元可以提高生长率。
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引用次数: 0
Concerns about the use of antimicrobials in swine herds and alternative trends 对猪群使用抗菌剂的担忧及替代趋势
IF 1.3 Q2 Veterinary Pub Date : 2024-03-16 DOI: 10.1093/tas/txae039
Luciana L Rigueira, Simone Percmanis
Pig productivity in Brazil has advanced a lot in recent decades. Specialized breeds are more vulnerable to pathogens, which has boosted the use of antimicrobials by farmers. The selective pressure generated favors the emergence of resistant bacteria, which compromises the effectiveness of this treatment and limits therapeutic options. In addition to increasing costs and mortality rates in the production system, public awareness of this issue has increased. The authorities have imposed restrictive measures to control the use of antimicrobials and have banned their use as growth promoters. This literature review highlights biosecurity and animal welfare to prevent pig diseases. Hence, we describe alternatives to the use of antimicrobials in pig production for the selection of effective non-antibiotic feed additives that help maintain good health and help the pig resist disease when infection occurs.
近几十年来,巴西的养猪业取得了长足的进步。专门的品种更容易受到病原体的侵袭,这促进了养殖户对抗菌素的使用。所产生的选择性压力有利于抗药性细菌的出现,从而损害了这种治疗方法的有效性,限制了治疗方案的选择。除了生产系统的成本和死亡率增加外,公众对这一问题的认识也有所提高。当局已采取限制性措施控制抗菌剂的使用,并禁止将其用作生长促进剂。本文献综述强调生物安全和动物福利以预防猪病。因此,我们介绍了在养猪生产中使用抗菌素的替代品,以选择有效的非抗生素饲料添加剂,帮助保持良好的健康状况,并在发生感染时帮助猪只抵抗疾病。
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引用次数: 0
Quantification of cooling effects on basic tissue measurements and exposed cross-sectional brain area of cadaver heads from Holstein cows > 30 mos of age 量化冷却对 30 月龄以上荷斯坦奶牛尸体头部基本组织测量值和暴露脑横截面积的影响
IF 1.3 Q2 Veterinary Pub Date : 2024-03-16 DOI: 10.1093/tas/txae042
Karly N Anderson, Emma M Hamilton, A. Kirk, Arquimides A Reyes, Ruth Woiwode, Perle E. Zhitnitskiy, K. D. Vogel
Penetrating captive bolt (PCB) is the primary method of pre-slaughter stunning for cattle and is also used for on-farm euthanasia. The objective of this study was to quantify the impact of cooling on the soft tissue thickness, cranial thickness, total tissue thickness, and cross-sectional brain area of cadaver heads collected from mature (> 30 mos of age) dairy cows following the application of a PCB stun in a frontal placement. Hide-on cadaver heads were obtained from culled dairy cows (N = 37) stunned in a frontal location using a handheld PCB device (Jarvis Model PAS – Type C 0.25R Caliber Captive Bolt, Long Bolt) at a commercial slaughter establishment. Following transport to the University of Wisconsin – River Falls, heads were split at midline along the bolt path by a bandsaw and then underwent FRESH, CHILL24, CHILL48, and CHILL72 refrigeration treatments. The FRESH treatment involved images collected immediately after splitting each head, the CHILL24 treatment involved images collected after 24 h of refrigeration, the CHIL48 treatment involved images collected after 48 h of refrigeration, and the CHILL72 treatment involved images collected after 72 h of refrigeration. Measurements of soft tissue thickness, cranial thickness, total tissue thickness, and cross-sectional brain area were recorded for each refrigeration treatment. Soft tissue thickness did not differ caudal to (P = 0.3751) or rostral to (P = 0.2555) the bolt path. Cranial thickness did not differ caudal to (P = 0.9281) or rostral to (P = 0.9051) the bolt path. Total tissue thickness did not differ caudal to (P = 0.9225; FRESH: 24.77 mm, CHILL24: 23.93 mm, CHILL48: 24.27 mm, CHILL72: 42.30, SE: 0.86) or rostral to (P = 0.8931; FRESH: 24.09 mm, CHILL24: 23.99, CHILL48: 24.26, CHILL72: 24.43 mm, SE: 0.79 mm) the bolt path. Cross-sectional brain area was not different (P = 0.0971) between refrigeration treatments (FRESH: 9,829.65 ± 163.87 mm2, CHILL24: 10,012.00 ± 163.87 mm2, CHILL48: 9,672.43 ± 163.87 mm2, CHILL72: 10,235.00 ± 166.34 mm2). This study demonstrated that FRESH tissue parameters can be determined from cattle cadaver heads refrigerated for 24, 48, or 72 h.
穿透式拴系法(PCB)是对牛进行宰前电击的主要方法,也用于农场安乐术。本研究的目的是量化冷却对软组织厚度、颅骨厚度、总组织厚度以及从成熟(大于 30 月龄)奶牛身上采集的头部横截面脑面积的影响。在一家商业屠宰场使用手持式多氯联苯装置(Jarvis Model PAS - Type C 0.25R Caliber Captive Bolt,长螺栓)对淘汰的奶牛(37 头)进行正面击晕后,从它们身上采集了藏在尸体上的头部。在运往威斯康星大学里弗福尔斯分校后,用带锯沿栓塞路径在中线劈开头部,然后进行FRESH、CHILL24、CHILL48和CHILL72冷冻处理。FRESH处理包括在劈开每个头部后立即采集图像,CHILL24处理包括在冷藏24小时后采集图像,CHIL48处理包括在冷藏48小时后采集图像,CHILL72处理包括在冷藏72小时后采集图像。每种冷藏处理都记录了软组织厚度、颅骨厚度、总组织厚度和大脑横截面积的测量值。软组织厚度在螺栓路径的尾部(P = 0.3751)和喙部(P = 0.2555)没有差异。颅骨厚度与螺栓路径尾部(P = 0.9281)或喙部(P = 0.9051)无差异。总组织厚度与螺栓路径尾侧(P = 0.9225;FRESH:24.77 mm,CHILL24:23.93 mm,CHILL48:24.27 mm,CHILL72:42.30,SE:0.86)或喙侧(P = 0.8931;FRESH:24.09 mm,CHILL24:23.99,CHILL48:24.26,CHILL72:24.43 mm,SE:0.79 mm)无差异。不同冷冻处理的大脑横截面积没有差异(P = 0.0971)(FRESH:9,829.65 ± 163.87 mm2;CHILL24:10,012.00 ± 163.87 mm2;CHILL48:9,672.43 ± 163.87 mm2;CHILL72:10,235.00 ± 166.34 mm2)。这项研究表明,可以从冷藏 24、48 或 72 小时的牛尸体头部测定 FRESH 组织参数。
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引用次数: 0
Energy efficiency of grazing Hereford heifers classified by paternal residual feed intake. 根据父代剩余饲料摄入量对放牧的赫里福德小母牛的能量效率进行分类。
IF 1.3 Q2 Veterinary Pub Date : 2024-03-14 eCollection Date: 2024-01-01 DOI: 10.1093/tas/txae005
María F Marín, Hugo Naya, Ana C Espasandin, Elly Navajas, Thais Devincenzi, Mariana Carriquiry

Residual feed intake (RFI) has become a widely spread index of feed efficiency. Although most of beef cattle systems in the world are pasture based, RFI evaluation and research is usually performed in confinement conditions. In this context, residual heat production (RHP) estimated as the difference between actual and expected heat production (HP), could allow to identify efficient animals. Thus, the aim of this work was to evaluate the relationship between paternal estimated breeding values (EBV) for RFI and beef heifer efficiency, measured as RHP, as well as its association with heifers' productive and reproductive performance on grazing conditions. Seventy-one 25 ± 0.8-mo-old and seventy-four 24 ± 0.7-mo-old Hereford heifers were managed as contemporary groups in spring 2019 and 2020, respectively. Heifers were sired by 10 RFI-evaluated bulls and classified into three groups according to the paternal EBV for RFI: five bulls of low RFI (high efficiency, pHE), two bulls of medium RFI (medium efficiency), and three bulls of high RFI (low efficiency, pLE). The experimental period lasted 70 d prior to their first insemination where HP was determined by the heart rate-O2 pulse technique. In addition, reproductive performances during the first and second breeding and calving seasons were recorded. Heifers' RHPs expressed as MJ/d and kJ/kg of body weight (BW)0.75/d were positively correlated with paternal RFI EBVs (P < 0.05; r > 0.60). Moreover, BW and average daily gain (ADG) were greater (P < 0.01) for pHE than pLE heifers while expressed as units of BW0.75/d, neither total HP nor metabolizable energy (ME) intake differed between groups, but pHE heifers had greater retained energy (RE; P < 0.01) and lower RHP (P < 0.05) than pLE ones. Gross energy efficiency (RE/ME intake) was greater (P < 0.001) for pHE than pLE heifers while the HP/ADG and RHP/ADG were reduced (P < 0.05) and feed-to-gain ratio (ADG/DM intake) tended to be greater (P = 0.07) for pHE than pLE heifers. In addition, during the first breeding and calving seasons, small but significant (P < 0.01) differences in reproductive responses between groups suggested an earlier pregnancy in pHE heifers than the pLE group, differences that disappeared during the second breeding and calving seasons. Thus, heifers sired by high-efficiency bulls measured as RFI were more efficient measured as RHP in grazing conditions, without significant differences in reproductive performance.

剩余采食量(RFI)已成为一种广泛应用的饲料效率指标。虽然世界上大多数肉牛饲养系统都以牧草为基础,但 RFI 评估和研究通常都是在圈养条件下进行的。在这种情况下,根据实际产热量与预期产热量(HP)之差估算的剩余产热量(RHP)可用于识别高效动物。因此,这项研究旨在评估RFI的父系估计育种值(EBV)与肉用小母牛效率(以RHP衡量)之间的关系,及其与小母牛在放牧条件下的生产性能和繁殖性能之间的关系。七十一头 25 ± 0.8 个月大和七十四头 24 ± 0.7 个月大的赫里福德小母牛分别于 2019 年和 2020 年春季作为当代组进行管理。母牛由 10 头经过 RFI 评估的公牛初配,并根据父系的 RFI EBV 分为三组:5 头低 RFI 的公牛(高效,pHE)、2 头中等 RFI 的公牛(中效)和 3 头高 RFI 的公牛(低效,pLE)。实验期为首次授精前 70 天,通过心率-O2 脉冲技术测定公牛的 HP 值。此外,还记录了第一、第二繁殖期和产犊期的繁殖性能。以 MJ/d 和 kJ/kg 体重 (BW)0.75/d 表示的母牛 RHP 与父亲 RFI EBV 呈正相关(P r > 0.60)。此外,体重和平均日增重(ADG)也更大(P 0.75/d),各组间总HP和可代谢能(ME)摄入量均无差异,但pHE小母牛的留存能(RE;P P P P = 0.07)高于pLE小母牛。此外,在第一个繁殖期和产犊期,pHE 小于 pLE 小于 pHE,但差异显著(P
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引用次数: 0
Pre-slaughter factors affecting mobility, blood parameters, bruising, and muscle pH of finished beef cattle in the United States 影响美国成品肉牛行动能力、血液参数、瘀伤和肌肉酸碱度的屠宰前因素
IF 1.3 Q2 Veterinary Pub Date : 2024-03-13 DOI: 10.1093/tas/txae035
Paxton Sullivan, Melissa Davis, Mahesh N Nair, Ann M Hess, Daniel F Mooney, L. Edwards-Callaway
Decades of work have focused on reducing fear, stress, and discomfort in cattle during the pre-slaughter phase by improving and promoting animal handling, transportation, and management processes. Even still, there is limited information about the effects of pre-slaughter factors on animal welfare and meat quality outcomes in finished cattle in the United States. This study aimed to track individual animals through the slaughter process to identify pre-slaughter factors associated with key welfare and quality outcomes. A total of 454 cattle from one commercial slaughter facility were studied. Pre-slaughter factors assessed included distance traveled, truck waiting time, lairage density, lairage duration, and season. Animal characteristics, i.e., body weight, breed, and sex, were also recorded. One trained observer scored the mobility of all cattle using the North American Meat Institute’s 1-4 scale (i.e., normal to extremely reluctant to move). Exsanguination blood was collected and analyzed for cortisol, creatine kinase, and lactate. Carcass bruising was scored using a modified version of the National Beef Quality Audit’s bruise scoring methodology (i.e., no bruise, one bruise ≤ the size of a deck of cards, one bruise > than the size of a deck of cards, and multiple bruises). Ultimate muscle pH was measured 32 to 36 hours postmortem. Multi-predictor models were selected for each outcome variable using Akaike Information Criterion. Continuous outcome variables were analyzed using linear mixed-effects models and categorical outcome variables with mixed-effects logistic regression models. Longer truck waiting times were associated with increased cortisol (P = 0.04) and lactate (P = 0.02) concentrations. Similarly, increased lairage duration was associated with increased creatine kinase (P = 0.05) and the odds of cattle being bruised (P = 0.03). Less space allowance per animal in lairage was associated with increased odds of cattle having impaired mobility (P = 0.01). There was a seasonal effect for many of the measured outcomes; the summer season was associated with greater lactate concentrations (P < 0.0001), increased odds of impaired mobility (P < 0.0001), and increased odds of carcass bruising (P = 0.003). The findings of this study indicate that many of the pre-slaughter factors assessed influence critical welfare and meat quality outcomes of finished beef cattle, warranting future research and consideration.
几十年来,人们一直致力于通过改进和促进动物处理、运输和管理流程,减少牛在屠宰前阶段的恐惧、压力和不适。尽管如此,有关屠宰前因素对美国成品牛的动物福利和肉质结果的影响的信息仍然有限。本研究旨在对屠宰过程中的动物个体进行跟踪,以确定与主要福利和质量结果相关的屠宰前因素。共对一家商业屠宰场的 454 头牛进行了研究。所评估的屠宰前因素包括牛群行进距离、卡车等待时间、屠宰场密度、屠宰场持续时间和季节。此外,还记录了动物的特征,即体重、品种和性别。一名训练有素的观察员使用北美肉类协会的 1-4 级评分表(即从正常到极不愿意移动)对所有牛的移动能力进行评分。采集血液并分析皮质醇、肌酸激酶和乳酸盐。胴体瘀伤采用国家牛肉质量审核的修改版瘀伤评分方法进行评分(即无瘀伤、一处瘀伤≤扑克牌大小、一处瘀伤大于扑克牌大小和多处瘀伤)。肌肉最终 pH 值在死后 32 至 36 小时测量。使用 Akaike 信息标准为每个结果变量选择多预测因子模型。连续结果变量采用线性混合效应模型进行分析,分类结果变量采用混合效应逻辑回归模型进行分析。卡车等待时间越长,皮质醇(P = 0.04)和乳酸(P = 0.02)浓度越高。同样,牲畜存栏时间的延长与肌酸激酶(P = 0.05)和牛只瘀伤几率(P = 0.03)的增加有关。每头牲畜在圈舍中的活动空间减少与牛的活动能力受损几率增加有关(P = 0.01)。许多测量结果都存在季节效应;夏季乳酸盐浓度较高(P < 0.0001),行动能力受损几率增加(P < 0.0001),胴体擦伤几率增加(P = 0.003)。本研究的结果表明,所评估的许多宰前因素都会影响成品肉牛的关键福利和肉质结果,值得今后研究和考虑。
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引用次数: 0
National Beef Quality Audit – 2022: Phase 1, face-to-face and digital interviews 国家牛肉质量审计 - 2022 年:第 1 阶段,面对面和数字访谈
IF 1.3 Q2 Veterinary Pub Date : 2024-03-12 DOI: 10.1093/tas/txae034
Colton L. Smith, Tyler W Thompson, Keayla M Harr, Macey Goretska, Thachary R Mayer, Trent E Schwartz, Sydni E Borders, K. Gehring, Phil D Bass, M. Pfeiffer, G. Mafi, D. Pendell, J. B. Morgan, D. Griffin, J. W. Savell, J. Scanga, M. N. Nair, K. Belk
The National Beef Quality Audit (NBQA) has been conducted regularly since 1991 to assess and benchmark quality in the U.S. beef industry, with the most recent iteration conducted in 2022. The goal of NBQA Phase I is to evaluate what needs to be managed to improve beef demand. Interviews (n = 130) of industry personnel were conducted with the aid of routing software. In total, packers (n = 24), retailers (n = 20), further processors (n = 26), foodservice (n = 18), and allied government agencies and trade organizations (GTO; n = 42) were interviewed. Interviews were routed in software based on interviewee involvement in either the fed steer and heifer or market cow and bull sectors, or both. Interviews were structured to elicit random responses in the order of determining “must-have” criteria (quality factors that are required to make a purchase), best/worst ranking (of quality factors based on importance), how interviewees defined quality terms, a Strengths, Weakness, Opportunities, Threats (SWOT) analysis, general beef industry questions, and sustainability goals (the latter four being open ended). Quality factors were 1) visual characteristics, 2) cattle genetics, 3) food safety, 4) eating satisfaction, 5) animal well-being, 6) weight and size, and 7) lean, fat, and bone. Best/Worst analysis revealed that “food safety” was the most (P < 0.05) important factor in beef purchasing decisions for all market sectors and frequently was described as “everything” and “a way of business.” Culture surrounding food safety changed compared to previous NBQAs with interviewees no longer considering food safety as a purchasing criterion, but rather as a market expectation. The SWOT analysis indicated that “eating quality of US beef” was the greatest strength, and cited that educating both consumers and producers on beef production would benefit the industry. Irrespective of whether companies’ products were fed or market cow/bull beef, respondents said that they believed “environmental concerns” were among the major threats to the industry. The market sectors’ perceived image of the beef industry has improved since NBQA-2016 for both fed cattle and market cow/bull beef.
自 1991 年以来,国家牛肉质量审核(NBQA)一直定期进行,以评估美国牛肉行业的质量并制定基准,最近一次审核于 2022 年进行。NBQA 第一阶段的目标是评估改善牛肉需求所需的管理措施。借助路由软件对行业人员进行了访谈(n = 130)。总共采访了包装商(n = 24)、零售商(n = 20)、深加工商(n = 26)、餐饮服务商(n = 18)以及相关政府机构和贸易组织(GTO;n = 42)。访谈在软件中根据受访者参与饲喂母牛和小母牛或市场母牛和公牛行业,或两者兼而有之的情况进行。访谈的结构是按照确定 "必备 "标准(购买所需的质量因素)、最佳/最差排序(根据重要性对质量因素进行排序)、受访者如何定义质量术语、优势、劣势、机会、威胁(SWOT)分析、一般牛肉行业问题和可持续发展目标(后四项为开放式)的顺序征求随机答复。质量因素包括:1)视觉特征;2)牛的遗传;3)食品安全;4)食用满意度;5)动物福利;6)重量和大小;7)瘦肉、脂肪和骨骼。最佳/最差分析显示,在所有市场领域,"食品安全 "是牛肉购买决策中最重要的因素(P < 0.05),并经常被描述为 "一切 "和 "一种经营方式"。与以往的 NBQAs 相比,围绕食品安全的文化发生了变化,受访者不再将食品安全作为采购标准,而是将其作为一种市场期望。SWOT 分析表明,"美国牛肉的食用品质 "是最大的优势,并指出对消费者和生产者进行牛肉生产方面的教育将使行业受益。无论公司的产品是饲料牛肉还是市场牛/公牛牛肉,受访者都认为 "环境问题 "是该行业面临的主要威胁之一。自 NBQA-2016 年以来,市场部门对牛肉行业的认知形象有所改善,无论是饲用牛还是市场牛/公牛牛肉。
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Translational Animal Science
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