A crossmatch test between patient and donor’s blood must be carried out before blood transfusion. However, not all crossmatched blood will be transfused to the patient. This indicates inappropriate distribution of blood products, blood wastage, inaccessibility of blood for emergency patients, increased costs, and increased workload for staff. Crossmatch to Transfusion Ratio (C/T Ratio) is used as an indicator to determine the efficiency of blood use and can serve as a guide to anticipate excessive blood demand. This study aims to determine C/T Ratio score of blood components WB and PRC in the Brebes Regency Hospital 2021. This research is a descriptive study with a retrospective approach. Sampling was done using total sampling technique. Data was obtained from the blood requests recapitulation book in Brebes Regency Hospital’s Blood Bank 2021. The result showed there were 47 units WB crossmatched and 34 units (72%) transfused. There were 4589 units PRC crossmatched and 4369 units (95%) transfused. The C/T Ratio score of WB is 1.38. The C/T Ratio score of PRC is 1.05. The overall C/T Ratio score is 1.05.
{"title":"Crossmatch to Transfusion Ratio (C/T Ratio) Komponen Darah WB dan PRC di RSUD Kabupaten Brebes","authors":"Valina Intan Safitri, M. S. A. S. Negoro","doi":"10.31983/jlm.v5i1.8587","DOIUrl":"https://doi.org/10.31983/jlm.v5i1.8587","url":null,"abstract":"A crossmatch test between patient and donor’s blood must be carried out before blood transfusion. However, not all crossmatched blood will be transfused to the patient. This indicates inappropriate distribution of blood products, blood wastage, inaccessibility of blood for emergency patients, increased costs, and increased workload for staff. Crossmatch to Transfusion Ratio (C/T Ratio) is used as an indicator to determine the efficiency of blood use and can serve as a guide to anticipate excessive blood demand. This study aims to determine C/T Ratio score of blood components WB and PRC in the Brebes Regency Hospital 2021. This research is a descriptive study with a retrospective approach. Sampling was done using total sampling technique. Data was obtained from the blood requests recapitulation book in Brebes Regency Hospital’s Blood Bank 2021. The result showed there were 47 units WB crossmatched and 34 units (72%) transfused. There were 4589 units PRC crossmatched and 4369 units (95%) transfused. The C/T Ratio score of WB is 1.38. The C/T Ratio score of PRC is 1.05. The overall C/T Ratio score is 1.05.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132462400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. xylene is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xylene is one of the most dangerous chemicals found in histology laboratories. Based on the dangers posed by xylene, a replacement material is needed. Several xylene substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xylene substitutes are still less effective, more expensive, and still as dangerous as xylene itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xylene as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xylene group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xylene and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xylene in the clearing process in histological tissue processing.
{"title":"Minyak Zaitun Sebagai Pengganti xylene pada Prosesing Jaringan Histologis Untuk Pewarnaan Kulit dan Hepar Mencit dengan Hematoxylin Eosin: Sebuah Studi Perbandingan","authors":"Eko Naning Sofyanita, Arya Iswara, Djoko Priyatno","doi":"10.31983/jlm.v4i2.8688","DOIUrl":"https://doi.org/10.31983/jlm.v4i2.8688","url":null,"abstract":"Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. xylene is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xylene is one of the most dangerous chemicals found in histology laboratories. Based on the dangers posed by xylene, a replacement material is needed. Several xylene substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xylene substitutes are still less effective, more expensive, and still as dangerous as xylene itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xylene as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xylene group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xylene and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xylene in the clearing process in histological tissue processing.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121699283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil Transmitted Helminths (STH) infection is still a health problem in Indonesia. This infection is caused by intestinal nematodes which in its transmission require soil media. Vegetables can be a medium of transmission of STH. STH transmission occurs when the soil is contaminated with STH worm eggs and the worm eggs stick to vegetables. In addition, STH contamination can occur in traditional markets with poor sanitation such as humid rooms, floors with lots of puddles, muddy water, and improper placement of waste. The aim of the study was to describe the contamination of Soil Transmitted Helminths on Scallion (Allium fistulosum L.). This research is a descriptive research, the examination of worm eggs is done by indirect method with sedimentation technique. The research sample was taken from 25 traders. Leeks contaminated with STH as many as 15 (60%) samples from 25 traders. The type of STH found was hookworm rhabditiform larvae. There is STH contamination in leeks sold at the Bangetayu market, Semarang City. To avoid the risk of being infected with STH, people are expected to consume vegetables that have been cooked.
{"title":"Contamination of Soil Transmitted Helminths (STH) on Leek (Allium fistulosum L.)","authors":"Dwi Ratna Cahyaningrum, Ririh Jatmi Wikandari","doi":"10.31983/jlm.v4i2.8351","DOIUrl":"https://doi.org/10.31983/jlm.v4i2.8351","url":null,"abstract":"Soil Transmitted Helminths (STH) infection is still a health problem in Indonesia. This infection is caused by intestinal nematodes which in its transmission require soil media. Vegetables can be a medium of transmission of STH. STH transmission occurs when the soil is contaminated with STH worm eggs and the worm eggs stick to vegetables. In addition, STH contamination can occur in traditional markets with poor sanitation such as humid rooms, floors with lots of puddles, muddy water, and improper placement of waste. The aim of the study was to describe the contamination of Soil Transmitted Helminths on Scallion (Allium fistulosum L.). This research is a descriptive research, the examination of worm eggs is done by indirect method with sedimentation technique. The research sample was taken from 25 traders. Leeks contaminated with STH as many as 15 (60%) samples from 25 traders. The type of STH found was hookworm rhabditiform larvae. There is STH contamination in leeks sold at the Bangetayu market, Semarang City. To avoid the risk of being infected with STH, people are expected to consume vegetables that have been cooked.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126328221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dina Afrianti, Elis Susilawati, Eko Naning Sofyanita
Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation
{"title":"Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor","authors":"Dina Afrianti, Elis Susilawati, Eko Naning Sofyanita","doi":"10.31983/jlm.v4i1.8445","DOIUrl":"https://doi.org/10.31983/jlm.v4i1.8445","url":null,"abstract":"Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"47 9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123172606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoki Setyaji, Tri Novitasari, Inggil Tunjung Muktianafi, Shintia Dini Anggreini, Norma Agustin Palupi
{"title":"Validation of Pneumatic Tube System Distance on The Results of Platelets and Potassium","authors":"Yoki Setyaji, Tri Novitasari, Inggil Tunjung Muktianafi, Shintia Dini Anggreini, Norma Agustin Palupi","doi":"10.31983/jlm.v4i1.8488","DOIUrl":"https://doi.org/10.31983/jlm.v4i1.8488","url":null,"abstract":"","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115340554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Trigycerides are the main lipid component in food intake, which is about 98% of total lipids and the remaining 2% consists of phospholipids and cholesterol. Triglycerides are present in adipose tissue, skeletal muscle, liver, lungs, and intestines to provide energy in metabolic processes. Who estimates about 1 bilion individuals are overweight and about 300 milion individuals are defined as obese, normal triglyceride levels usually do not exceed 150 mg/dl, if triglyceride levels increase by more than 200 mg/dl then called hypertriglyceridemia. The research aims to determine the difference in triglceride levels using serum and plasma EDTA conducted at the Center For Health Laboratories (BBLK) Palembang. The type of research used is cross Sectional with research designintact group comparison. The sample consisted of 30 serums and 30 plasma EDTA taken from 30 respondents. Research is carried out starting from the preparation of patients, taking examination materials, processing of examination materials, analysis and results of research. The average levels of triglyceride examination using serum and plasma were obtained consecutively at 0.91 mmol/L and 0.83 mmol/L. Mann Whitney test results found that the significant value was p = 0.240. The p value obtained is p0.05. The results can be concluded that there is no difference in examination of triglyceride levels using serum and plasma EDTA.
{"title":"Perbedaan Kadar Trigliserida Menggunakan Serum dan Plasma Edta dengan Biosystem A15","authors":"Sarah Aminah, Indah Sari, Bastian Bastian","doi":"10.31983/jlm.v4i1.8402","DOIUrl":"https://doi.org/10.31983/jlm.v4i1.8402","url":null,"abstract":"Trigycerides are the main lipid component in food intake, which is about 98% of total lipids and the remaining 2% consists of phospholipids and cholesterol. Triglycerides are present in adipose tissue, skeletal muscle, liver, lungs, and intestines to provide energy in metabolic processes. Who estimates about 1 bilion individuals are overweight and about 300 milion individuals are defined as obese, normal triglyceride levels usually do not exceed 150 mg/dl, if triglyceride levels increase by more than 200 mg/dl then called hypertriglyceridemia. The research aims to determine the difference in triglceride levels using serum and plasma EDTA conducted at the Center For Health Laboratories (BBLK) Palembang. The type of research used is cross Sectional with research designintact group comparison. The sample consisted of 30 serums and 30 plasma EDTA taken from 30 respondents. Research is carried out starting from the preparation of patients, taking examination materials, processing of examination materials, analysis and results of research. The average levels of triglyceride examination using serum and plasma were obtained consecutively at 0.91 mmol/L and 0.83 mmol/L. Mann Whitney test results found that the significant value was p = 0.240. The p value obtained is p0.05. The results can be concluded that there is no difference in examination of triglyceride levels using serum and plasma EDTA.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126540513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fresh vegetables can be a source of transmission of worm infections to humans. The behavior of washing fresh vegetables that are not good by housewives and sellers of fresh vegetables in food stalls becomes large in transmitting worm infections if there are worm eggs that are still attached to fresh vegetables. The most common contamination found in fresh vegetables were eggs of the intestinal nematode Soil Transmitted Helminths (STH) as well as non-STH and trematodes. The purpose of this study was to determine the relationship between sanitation hygiene and contamination of non-STH worm eggs in fresh vegetables at food stalls in Semarang City. This type of research is an observational study with a cross sectional approach. Based on the results of laboratory tests, from a total of 165 samples examined, eggs of non-STH worms with species of Fasciola sp and Toxocara sp were found in 45 food stalls selling fresh vegetables. Based on bivariate analysis, there was no significant relationship between personal hygiene (p = 0.071) and environmental sanitation (p = 0.334) on contamination of non-STH worm eggs in fresh vegetables in food stalls in Pedurungan District, Semarang.
{"title":"Hubungan Antara Higiene Sanitasi Terhadap Kontaminasi Telur Cacing Non STH Pada Sayur Lalapan di Warung Makan","authors":"Lilik Setyowatiningsih, Ririh Jatmi Wikandari, Surati Surati","doi":"10.31983/jlm.v4i1.8325","DOIUrl":"https://doi.org/10.31983/jlm.v4i1.8325","url":null,"abstract":"Fresh vegetables can be a source of transmission of worm infections to humans. The behavior of washing fresh vegetables that are not good by housewives and sellers of fresh vegetables in food stalls becomes large in transmitting worm infections if there are worm eggs that are still attached to fresh vegetables. The most common contamination found in fresh vegetables were eggs of the intestinal nematode Soil Transmitted Helminths (STH) as well as non-STH and trematodes. The purpose of this study was to determine the relationship between sanitation hygiene and contamination of non-STH worm eggs in fresh vegetables at food stalls in Semarang City. This type of research is an observational study with a cross sectional approach. Based on the results of laboratory tests, from a total of 165 samples examined, eggs of non-STH worms with species of Fasciola sp and Toxocara sp were found in 45 food stalls selling fresh vegetables. Based on bivariate analysis, there was no significant relationship between personal hygiene (p = 0.071) and environmental sanitation (p = 0.334) on contamination of non-STH worm eggs in fresh vegetables in food stalls in Pedurungan District, Semarang.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133067042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Uncontrolled Diabetes Mellitus will lead to long-term complications, namely decreased organ function, especially the kidneys, nerves, eyes, blood vessels and heart. Diabetic nephropathy is a complication that is often found in DM patients and attacks the kidneys. Serum creatinine examination is used to assess kidney function in patients with Type 2 Diabetes Mellitus. This purpose of research is to describe the description of serum creatinine levels in inpatients diagnosed with Type 2 Diabetes Mellitus at Dr. Kariadi Semarang. This study uses descriptive quantitative. The data comes from the medical records of inpatients diagnosed with Type 2 DM at Dr. RSUP. Kariadi Semarang who examined creatinine levels in the period January - April 2020 with the total sampling method. From this study, inpatients diagnosed with Type 2 DM at Dr. Kariadi Semarang were 79 respondents with male sex as many as 49 people (62%) and women 30 people (38%). In 79 respondesnts, there are 2 respondents (2.5%) had low creatinine levels, 7 respondents (8.9%) had normal creatinine levels, while 70 respondents (91.1%) had high creatinine levels. 43 male patients (88.6%) were included in the category of high creatinine levels. Based on age, it was dominated by the late elderly (55 – 65 years) as many as 32 people (40.5%) with the late elderly patients as many as 28 people (87.5%) having high creatinine levels. Conclusion: In 79 respondesnts (100%), there are 72 respondents (91.1%) who have abnormal creatinine levels and 7 respondents (8.9%) have normal creatinine levels.
{"title":"Gambaran Kadar Kreatinin Serum Pada Pasien Rawat Inap Yang Didiagnosis Diabetes Melitus Tipe 2","authors":"Tyas Pertiwi Armaningrum, Teguh Budiharjo","doi":"10.31983/jlm.v4i1.8352","DOIUrl":"https://doi.org/10.31983/jlm.v4i1.8352","url":null,"abstract":"Uncontrolled Diabetes Mellitus will lead to long-term complications, namely decreased organ function, especially the kidneys, nerves, eyes, blood vessels and heart. Diabetic nephropathy is a complication that is often found in DM patients and attacks the kidneys. Serum creatinine examination is used to assess kidney function in patients with Type 2 Diabetes Mellitus. This purpose of research is to describe the description of serum creatinine levels in inpatients diagnosed with Type 2 Diabetes Mellitus at Dr. Kariadi Semarang. This study uses descriptive quantitative. The data comes from the medical records of inpatients diagnosed with Type 2 DM at Dr. RSUP. Kariadi Semarang who examined creatinine levels in the period January - April 2020 with the total sampling method. From this study, inpatients diagnosed with Type 2 DM at Dr. Kariadi Semarang were 79 respondents with male sex as many as 49 people (62%) and women 30 people (38%). In 79 respondesnts, there are 2 respondents (2.5%) had low creatinine levels, 7 respondents (8.9%) had normal creatinine levels, while 70 respondents (91.1%) had high creatinine levels. 43 male patients (88.6%) were included in the category of high creatinine levels. Based on age, it was dominated by the late elderly (55 – 65 years) as many as 32 people (40.5%) with the late elderly patients as many as 28 people (87.5%) having high creatinine levels. Conclusion: In 79 respondesnts (100%), there are 72 respondents (91.1%) who have abnormal creatinine levels and 7 respondents (8.9%) have normal creatinine levels.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132885307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.
{"title":"Rasio Penutupan Luka pada Tikus Diabetes Diinduksi Streptozotocin dengan Perlakuan Dressing Tipe Pasif dan Interaktif (Penelitian Pendahuluan)","authors":"Eko Naning Sofyanita, Arya Iswara","doi":"10.31983/jlm.v3i2.8045","DOIUrl":"https://doi.org/10.31983/jlm.v3i2.8045","url":null,"abstract":"Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122897063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The metal smelting industry tends to have high air Pb levels exceeding the threshold determined by the CDC is 100 g/m3. The smelting dust particles are Pb and SO2 circulating in the air along with combustion fumes is very possible to be inhaled by metal smelters who do not wear proper personal protective equipment. High levels of Pb The air inhaled by metal smelters can affect the health level of workers, one of which is the disruption of the erythropoiesis process which can cause abnormal erythrocyte index levels. This study aims to determine the description of erythrocyte index levels in metal smelters based on literature studies. This research is an observational qualitative research through a literature review approach, with data collection methods that use secondary data from several related scientific journals. Secondary data analysis is descriptive and presented in tabular form. Based on the results of research from three related scientific journals, the average level of the erythrocyte index in metal smelting workers is normochromic normocytic with an average length of work of more than 3.89 years, workers who smoke as much as 78% and those who do not smoke as much as 22%, workers who using PPE as much as 56% and 44% who do not use PPE.
{"title":"Erythrocyte Index Levels in Metal Smelters Based on Literature Studies","authors":"Widhi Ulya Syamputri","doi":"10.31983/jlm.v3i1.7960","DOIUrl":"https://doi.org/10.31983/jlm.v3i1.7960","url":null,"abstract":"The metal smelting industry tends to have high air Pb levels exceeding the threshold determined by the CDC is 100 g/m3. The smelting dust particles are Pb and SO2 circulating in the air along with combustion fumes is very possible to be inhaled by metal smelters who do not wear proper personal protective equipment. High levels of Pb The air inhaled by metal smelters can affect the health level of workers, one of which is the disruption of the erythropoiesis process which can cause abnormal erythrocyte index levels. This study aims to determine the description of erythrocyte index levels in metal smelters based on literature studies. This research is an observational qualitative research through a literature review approach, with data collection methods that use secondary data from several related scientific journals. Secondary data analysis is descriptive and presented in tabular form. Based on the results of research from three related scientific journals, the average level of the erythrocyte index in metal smelting workers is normochromic normocytic with an average length of work of more than 3.89 years, workers who smoke as much as 78% and those who do not smoke as much as 22%, workers who using PPE as much as 56% and 44% who do not use PPE.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121871413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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