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Perbandingan Berbagai Metode Pengecatan Spora pada Bacillus Cereus 将芽孢杆菌的各种油漆方法进行比较
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v4i2.8493
W. Widodo, Devi Etivia Purlinda, Ahmad Riadi
Endospores are formed by members of several genera of gram-positive bacteria, such as Bacillus and Clostridium. The spores are very resistant structurally; withstand the temperature of boiling water for two hours or more. They contain very little water and show very few chemical reactions. The favorable external environment will cause the spores to break down and the vegetative cells to appear to grow and reproduce. Identification of endospore-forming pathogens is important in food and medical microbiology. However, the spores contain multiple protective layers, which cannot be easily penetrated by simple staining or Gram staining techniques. Therefore, it is necessary to apply heat to help penetrate the color, with various methods of Gram stain, fluorescent spore painting, Schaeffer and Fulton spore painting and Client's spore painting method. The results of the fluorescent painting method have better results but require much more expensive equipment using a fluorescent microscope considering the facilities that must be provided for the Schaeffer and Fulton painting method as an alternative if they do not have these facilities.
内孢子是由革兰氏阳性细菌的几个属的成员形成的,如芽孢杆菌和梭状芽孢杆菌。孢子在结构上具有很强的抵抗力;能承受沸水的温度两小时或更长时间。它们含有很少的水,很少发生化学反应。良好的外部环境会使孢子分解,使营养细胞出现生长和繁殖。孢子内形成病原体的鉴定在食品和医学微生物学中具有重要意义。然而,孢子含有多层保护层,不容易通过简单染色或革兰氏染色技术穿透。因此,需要通过加热来帮助穿透颜色,采用革兰氏染色法、荧光孢子涂布法、谢弗和富尔顿孢子涂布法、Client孢子涂布法等多种方法。荧光绘画方法的结果有更好的结果,但考虑到必须为Schaeffer和Fulton绘画方法提供的设施,如果他们没有这些设施,则需要使用荧光显微镜的昂贵得多的设备。
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引用次数: 0
Gambaran Hemoglobin pada Pendonor yang Ditolak di PMI Tahun 2020-2021 dan Faktor yang Mempengaruhi 2010 -2021年PMI上被拒绝的献血者的血红蛋白表现及其影响因素
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v5i1.9288
Hendar Fahmi Ananda, Miftahur Rizqiya Sekar Jati, Dinda Ayu Mustika Putri, Husna Azia, Zainul Akbar, Rodhi Hartono
Hemoglobin or Hb is a protein found in red blood cells. This protein is what makes blood red. In normal levels, hemoglobin has many functions for the body, so normal levels of hemoglobin need to be maintained. In addition to giving color, hemoglobin also functions to help red blood cells get their natural shape, which is round with a flatter center. With this shape, red blood cells can easily move and flow in the blood vessels. Based on the Regulation of the Minister of Health Number 91 of 2015 the normal level of hemoglobin before blood donation is 12.5 - 17.0 gr/dL%. When a person's hemoglobin condition is higher or lower than the normal amount, this can be a sign of a health problem.
血红蛋白(Hb)是一种在红细胞中发现的蛋白质。这种蛋白质使血液变红。在正常水平下,血红蛋白对身体有许多功能,因此需要维持正常水平的血红蛋白。除了赋予颜色外,血红蛋白还能帮助红细胞获得其自然形状,即圆心较平的形状。有了这种形状,红细胞可以很容易地在血管中移动和流动。根据卫生部2015年第91号条例,献血前血红蛋白的正常水平为12.5 - 17.0克/分升%。当一个人的血红蛋白水平高于或低于正常水平时,这可能是健康问题的征兆。
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引用次数: 0
Profile of Fasting Blood Glucose Levels with and Without Drinking Water 空腹血糖水平与不喝水的关系
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v4i2.8471
Karenima Eka Wahyu Nurrois
Blood glucose levels are influenced by factors related to pastients and laboratory factors. There are various ways to treat blood glucose that have increased or decreased. Water consumption helps the process of removing all toxins in the body, including excess sugar. In James’s research (2010), that drinking water causes a breakdown of sugar. This research is to Know the description of fasting blood glucose levels with and without drinking water. Descriptive type research with cross sectional approach. Blood glucose levels of 28 student of TLM was examined by POCT methode and the respondent filled out the questionnare. The results of blood glucose levels are described based on questionnares that have been filled in the form of respondents diagram. From the results of examination 28 respondents, mean blood glucose level in conditions without drinking is 82,03 mg/dl, respondents with a minimum level of 65 mg/dl and a maximum of 97 mg/dl. Mean blood glucose level in conditions with drinking is 78,71 mg/dl, respondents with minimum level of 69 mg/dl and maximum of 104 mg/dl. Respondent’s fasting blood glucose levels in conditions without drinking and drinking decreased by 4%. There was a decrease in fasting blood glucose levels in respondents conditions without drinking and by drinking water, also influenced by physical activity factors, nutrition intake, drugs/vitamin and obesity.
血糖水平受患者相关因素和实验室因素影响。治疗血糖升高或降低的方法有很多。喝水有助于清除体内所有毒素,包括多余的糖。在James的研究(2010)中,喝水会导致糖的分解。本研究的目的是了解空腹和不喝水时血糖水平的描述。用横断面方法进行描述性研究。采用POCT法检测28名TLM学生的血糖水平,并填写问卷。血糖水平的结果是根据问卷来描述的,问卷以受访者图表的形式填写。从28名调查对象的检查结果来看,在不饮酒的情况下,平均血糖水平为82,03 mg/dl,调查对象的最低水平为65 mg/dl,最高水平为97 mg/dl。饮酒条件下的平均血糖水平为78,71 mg/dl,应答者最低水平为69 mg/dl,最高水平为104 mg/dl。被调查者在不饮酒和饮酒的情况下空腹血糖水平下降了4%。在不喝水和喝水的情况下,受访者的空腹血糖水平有所下降,这也受到身体活动因素、营养摄入、药物/维生素和肥胖的影响。
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引用次数: 0
Uric Acid Levels in Elderly Gymnastics Participants at the Elderly Posyandu 老年体操运动员尿酸水平测定
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v4i2.8485
Fransiska Pangalissani
Process of getting older causes a person to experience physical and mental, spiritual, economic and social changes as well as degeneration of organ function. Age is one of the risk factors for high uric acid. Uric acid is the final product or waste product from purine metabolism, that excess amount in the blood will crystallize. At an advanced age, there will be a decrease in the production of several enzymes and hormones in the body that play a role in the process of removing gout. Exercise such as elderly gymnastic regularly can delay physiological changes that usually occur in a decrease in strength and flexibility, as well as an increased susceptibility to injury. The results are presented in descriptive form which supported by tables to describe uric acid levels and its causative factors in elderly gymnastic participants at the elderly posyandu in Village Timang, Sub District Wonokerto, District Wonogiri.  Examination of uric acid levels from 73 respondents were 42 respondents (57.5%) with normal uric acid levels and 31 respondents (42.5%) with uric acid levels above normal. An increase in uric acid levels can be caused by several factors such as age, sex, food intake, physical activity and history.
随着年龄的增长,人的身心、精神、经济和社会都会发生变化,器官功能也会退化。年龄是高尿酸的危险因素之一。尿酸是嘌呤代谢的最终产物或废物,血液中过量的尿酸会结晶。在老年时,体内几种酶和激素的产生会减少,这些酶和激素在消除痛风的过程中起作用。像老年人经常做体操这样的运动可以延缓通常发生在力量和柔韧性下降的生理变化,以及对受伤的易感性增加。结果以描述性的形式呈现,并以表格的形式描述了沃诺基里区沃诺基托街道timmang村老年健身中心老年体操参与者的尿酸水平及其致病因素。对73名受访者的尿酸水平进行检查,其中42人(57.5%)尿酸水平正常,31人(42.5%)尿酸水平高于正常。尿酸水平的升高可由几个因素引起,如年龄、性别、食物摄入、身体活动和历史。
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引用次数: 0
Uji Efektivitas Air Perasan Daun Jeruk Limau Kuit ( Citrus hystrix ) Sebagai Insektisida Nabati Terhadap Mortalitas Kutu Rambut Pediculus humanus capitis Secara In Vitro 测试水压榨柑橘(Citrus hystrix)作为一种针对根毛跳蚤在体外的形态形态的植物杀虫剂的有效性
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v4i2.8579
Nidha Pusvita, Anny Thuraidah, Rifqoh Rifqoh, Dinna Rakhmina
Lemongrass leaves are a typical plant of South Kalimantan which contain anti-insecticide, namely secondary metabolites such as alkaloids, saponins, tannins, steroids and triterpenoids. Pediculosis is a disease caused by a tick infection which is still neglected and becomes a health problem. One way to get rid of head lice is to use natural ingredients that contain anti-insecticide substances. This study aims to examine the possibility of using natural ingredients in lime leaves as vegetable insecticides against head lice mortality. The research method used is experimental, with a research design that is a post-test only control group, which measures and compares the mortality variable of Pediculus humanus capitis after being treated with lime leaf juice with concentrations of 10%, 30%, 60%, 100% during 1, 6, 12 and 24 hours and compared them with the negative control group with aquadest and the positive control with pemethrin. The sample of this study was lime leaf extract which was extracted by the method of squeezing using water. From the results of the study obtained the highest percentage of death at 12 and 24 hours with 100% mortality at a concentration of 100%. Based on the results of statistical tests using mortality observation data, the LC99 values were obtained within 1 hour (197.678%), 6 hours (126.055%), 12 hours (89.847%), and 24 hours (60.758%). Based on the results of the analysis of the data obtained, it can be concluded that there is effectiveness of lime leaf juice (Citrus hystrix) on the mortality of head lice (Pediculus humanus capitis) in vitro. Then the juice of lime leaves can be used as an alternative to getting rid of head lice with natural ingredients.
柠檬草叶是南加里曼丹的一种典型植物,含有抗杀虫剂,即生物碱、皂苷、单宁、类固醇和三萜等次生代谢产物。蜱虫病是一种由蜱虫感染引起的疾病,它仍然被忽视,成为一个健康问题。去除头虱的一种方法是使用含有抗杀虫剂物质的天然成分。本研究旨在探讨利用酸橙叶中的天然成分作为蔬菜杀虫剂防治头虱死亡率的可能性。采用实验研究方法,研究设计为试验后仅设对照组,测量并比较浓度为10%、30%、60%、100%的酸橙叶汁在1、6、12、24 h处理人头蒂后的死亡率变量,并与阴性对照组用阿奎德酯和阳性对照组用氯菊酯进行比较。本研究的样品为石灰叶提取物,采用水挤压法提取。研究结果显示,12小时和24小时死亡率最高,浓度为100%时死亡率为100%。采用死亡观察资料进行统计检验,LC99值分别为1 h(197.678%)、6 h(126.055%)、12 h(89.847%)和24 h(60.758%)。根据所获得数据的分析结果,可以得出结论,酸橙叶汁(Citrus hystrix)对头虱(人头虱)的体外死亡率有一定的效果。然后,酸橙叶的汁液可以作为一种替代方法,用天然成分去除头虱。
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引用次数: 0
Pengaruh Lama Penyimpanan Jamu Beras Kencur terhadap Pertumbuhan Kapang 营养素对马铃薯生长的长期影响
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v4i2.8571
Fransiskus Xaverius Agung Pinto Laksono, Ratih Dewi Dwiyanti, ri Rifqoh, Aima Insana
During the Covid-19 pandemic, people began to maintain their health, by using herbal medicine as an alternative treatment. One type of herbal medicine used is jamu beras kencur because it has properties to increase stamina, increase appetite, and relieve aches and cough disorders. In the Regulation of the Minister of Health of the Republic of Indonesia No. 007 of 2012 carrying herbs such as jamu beras kencur does not require marketing authorization. The quality of the herbal medicine produced has not been confirmed as feasible from microbial contamination, especially in molds, the number of yeast molds in the jamu beras kencur must comply with BPOM standards Number 32 of 2019 namely AKK 103 colonies/mL. This study aimed to determine the effect of the storage time of jamu beras kencur on mold growth with storage treatments of 0 hours, 4 hours, 8 hours, 12 hours, and 24 hours at room temperature. This type of research is a Quasi Experiment with a research design of One Group Pretest-Postest Design. The results of this study showed that the average AKK value was 0 hours 0.2x100 colonies/mL, 4 hours 0.4x100 colonies/mL, 8 hours 0.4x100 colonies/mL. 12 hours 0.8x100 colonies/mL and 24 hours 2.2x100 colonies/mL. These results stated that the jamu beras kencur met the BPOM standard requirements Number 32 of 2019 which was AKK 103 Colonies/mL. The results of the Kruskal Wallis test obtained a sig value of 0.286, it was concluded that there was no effect of storage time for the jamu beras kencur on the growth of molds. The identification results on the jamu beras kencur obtained fungal species, namely Aspergillus flavus, Aspergillus niger, and Penicillium sp. Suggestions for further researchers can examine the effect of storage time on mold growth in other types of herbal medicine.
在2019冠状病毒病大流行期间,人们开始通过使用草药作为替代治疗来保持健康。使用的一种草药是jamu beras kencur,因为它具有增强耐力,增加食欲和缓解疼痛和咳嗽疾病的特性。在2012年第007号印度尼西亚共和国卫生部长条例中,携带诸如牛膝草之类的草药不需要销售许可。所生产的草药的质量尚未得到微生物污染的证实,特别是在霉菌中,jamu beras kencur中的酵母菌数量必须符合2019年第32号BPOM标准,即AKK 103菌落/mL。本研究旨在确定在室温下贮藏0小时、4小时、8小时、12小时和24小时对赤霉病菌生长的影响。本研究为准实验,研究设计为一组前测后测设计。本研究结果表明,平均AKK值为0小时0.2 × 100菌落/mL, 4小时0.4 × 100菌落/mL, 8小时0.4 × 100菌落/mL。12小时0.8 × 100菌落/mL, 24小时2.2 × 100菌落/mL。这些结果表明,jamu beras kencur符合2019年第32号BPOM标准要求,即AKK 103菌落/mL。Kruskal - Wallis检验的sig值为0.286,说明贮藏时间对霉菌生长没有影响。对黄曲霉、黑曲霉、青霉等真菌种类的鉴定结果表明,对进一步研究人员的建议可以进一步研究其他类型中草药中储存时间对霉菌生长的影响。
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引用次数: 0
Profile of Volunteer Blood Donation during The Month of Ramadhan 斋月期间志愿献血概况
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v5i1.9147
Astri Dewi Ayu Kartika, Adita Puspitasari Swastya Putri
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引用次数: 0
Perbandingan Kadar Ureum Sebelum dan Sesudah Hemodialisis Pada Penderita Gagal Ginjal Kronik 慢性肾衰竭患者尿素前后的比较
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v5i1.9161
Imam Sudarso, Retno Sulistiyowati, Minto Rahaju, Tantri Analisawati Sudarsono
One of the body's organs, the kidney serves a vital purpose in the excretory and secretory systems. Excretion is the removal of waste products from the body's metabolism, and secretion is the release of chemicals and hormones that are involved in the metabolism of the body. Kidney failure is a condition brought on by kidney damage and is further broken down into acute and chronic forms. Long-lasting kidney damage carries a higher risk to one's health. Hemodialysis is a type of dialysis that helps the kidneys perform their normal functions by removing waste and toxic substances that build up in the blood. This study is analytical observational with a cross-sectional design and a sample of patients with chronic kidney failure at RSUD (Regional Public Hospital) dr. R. Goeteng Taroenadibrata Purbalingga. Non-probability sampling with a purposive sampling method was the sampling technique used. This study compared the urea levels in patients with chronic kidney failure before and after hemodialysis. According to the results of the study, there is a significant difference between urea levels before and after hemodialysis, which had a p-value of 0.003 (sig 0.05).
肾脏是人体器官之一,在排泄和分泌系统中起着至关重要的作用。排泄是身体新陈代谢过程中废物的排出,而分泌是与身体新陈代谢有关的化学物质和激素的释放。肾衰竭是一种由肾脏损伤引起的疾病,分为急性和慢性两种。长期的肾脏损伤会给健康带来更高的风险。血液透析是一种通过清除积聚在血液中的废物和有毒物质来帮助肾脏发挥正常功能的透析。本研究采用横断面设计和RSUD(地区公立医院)dr. Goeteng Taroenadibrata Purbalingga的慢性肾衰竭患者样本进行分析观察。采用非概率抽样和目的性抽样方法进行抽样。本研究比较了慢性肾衰竭患者血液透析前后的尿素水平。研究结果显示,血液透析前后尿素水平差异有统计学意义,p值为0.003 (sig 0.05)。
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引用次数: 0
Identifikasi Bakteri pada Luka Penderita Diabetes Melitus di Rumah Perawatan Luka Diabetes 糖尿病患者在糖尿病治疗室的瘀伤中发现的细菌
Pub Date : 1900-01-01 DOI: 10.31983/jlm.v5i1.8780
Venny Patricia, Ahmad Yani, Salwa Salsabila, Rr.Sri Isjworowati
Diabetes mellitus (DM) is a metabolic disease that occurs due to hyperglycemia. In Indonesia it self, DM has a relatively high prevalence, this is evidenced because Indonesia is in the category of the top 10 countries in the world. The type of research used is descriptive research, namely identifying bacteria and testing sensitivity on wounds of diabetics with diabetes mellitus by performing cultures that are examined macroscopically and microscopically with the number of respondents obtained by as many as 17 people in 4 weeks. The results of this study obtained the prevalence of the types of bacteria that are (82.35%) gram-negative and (17.65%) gram-positive. The gram negatives included Proteus mirabilis (17.65%), Proteus morgani (5.88%), Citrobacter diversus (23.54%), Pseudomonas aeruginosa (11.76%), Escherichia coli (5.88%), Enterobacter agglomerans (5.88%) and Enterobacter cloacae (11.76%). While the gram-positive include Staphylococcus aureus (17.65%) with antibiotic sensitivity results whose sensitivity is in the yellow zone, which is around 50-75%, namely ciprofloxacin and meropenem antibiotics are in the green zone, which is around 76-100%.
糖尿病(DM)是一种由高血糖引起的代谢性疾病。在印度尼西亚本身,糖尿病的患病率相对较高,这一点得到了证明,因为印度尼西亚是世界上排名前十的国家之一。所使用的研究类型是描述性研究,即通过进行宏观和微观检查的培养来鉴定糖尿病患者伤口上的细菌和测试敏感性,调查人数在4周内多达17人。本研究结果显示,革兰氏阴性菌(82.35%)和革兰氏阳性菌(17.65%)的流行率。革兰氏阴性包括奇异变形杆菌(17.65%)、摩根变形杆菌(5.88%)、多样柠檬酸杆菌(23.54%)、铜绿假单胞菌(11.76%)、大肠杆菌(5.88%)、聚集肠杆菌(5.88%)和阴沟肠杆菌(11.76%)。革兰氏阳性包括金黄色葡萄球菌(17.65%),其抗生素敏感性结果在黄色区域,约为50-75%,即环丙沙星和美罗培南抗生素在绿色区域,约为76-100%。
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引用次数: 0
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Jaringan Laboratorium Medis
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