Objective: V617F mutation of Janus kinase (JAK2) gene is used in the diagnosis of BCR-ABL negative myeloproliferative diseases such as polycytemia vera, essential thrombocythemia and primary myelofibrosis. In this study, we evaluated the frequency of JAK2 V617F mutation in a group of Turkish individuals and its association with whole blood count parameters. Methods: We retrospectively reviewed the records of 201 patients (174 males and 27 females) who were tested for JAK2 V617F mutation from January 2012 to December 2012. JAK2V617F mutation, BCR-ABL mutation, level of white blood cell count (WBC), red blood cell count (RBC), platelet count (PLT), red blood cell distribution width (RDW), hemoglobin (Hb), hematocrit (Hct), level of iron, iron binding capacity and demographic data of patients were also noted. A commercial kit assay for the identification of V617F mutation based on real time-polymerase chain reaction had been used to investigate mutation of the JAK2 gene. Results: 14.9 % of the 201 samples being investigated for JAK2 V617F mutation were positive. Patients who were ordered for JAK2 V617F mutation testing were divided into two groups according to mutation being positive and negative. Age, RDW and PLT levels were significantly higher in mutation positive group (p<0.05). Besides JAK2 V617F mutation, BCR-ABL mutation orders were examined and BCR-ABL mutation reports were also evaluated on subjects. Age, RDW and PLT were significantly higher in JAK2 V617F mutation positive / BCR-ABL mutation negative group (p<0.05). Conclusion: In this study significant differences for age, RDW and platelet count were determined in mutation positive subjects. Findings of this study suggest that JAK2 V617F mutation could cause proliferation of megakaryocytic cells as well as erythroid cells and as a result of disturbance in erythroid maturation process, the proliferation in the erythroid cells could cause an increase in RDW.
{"title":"The Frequency Of JAK2 V617F Gen Mutation And Its Associations With Whole Blood Count Parameters","authors":"Kübranur Ünal, S. Erdogan, F. Yilmaz","doi":"10.5505/TJB.2014.39206","DOIUrl":"https://doi.org/10.5505/TJB.2014.39206","url":null,"abstract":"Objective: V617F mutation of Janus kinase (JAK2) gene is used in the diagnosis of BCR-ABL negative myeloproliferative diseases such as polycytemia vera, essential thrombocythemia and primary myelofibrosis. In this study, we evaluated the frequency of JAK2 V617F mutation in a group of Turkish individuals and its association with whole blood count parameters. Methods: We retrospectively reviewed the records of 201 patients (174 males and 27 females) who were tested for JAK2 V617F mutation from January 2012 to December 2012. JAK2V617F mutation, BCR-ABL mutation, level of white blood cell count (WBC), red blood cell count (RBC), platelet count (PLT), red blood cell distribution width (RDW), hemoglobin (Hb), hematocrit (Hct), level of iron, iron binding capacity and demographic data of patients were also noted. A commercial kit assay for the identification of V617F mutation based on real time-polymerase chain reaction had been used to investigate mutation of the JAK2 gene. Results: 14.9 % of the 201 samples being investigated for JAK2 V617F mutation were positive. Patients who were ordered for JAK2 V617F mutation testing were divided into two groups according to mutation being positive and negative. Age, RDW and PLT levels were significantly higher in mutation positive group (p<0.05). Besides JAK2 V617F mutation, BCR-ABL mutation orders were examined and BCR-ABL mutation reports were also evaluated on subjects. Age, RDW and PLT were significantly higher in JAK2 V617F mutation positive / BCR-ABL mutation negative group (p<0.05). Conclusion: In this study significant differences for age, RDW and platelet count were determined in mutation positive subjects. Findings of this study suggest that JAK2 V617F mutation could cause proliferation of megakaryocytic cells as well as erythroid cells and as a result of disturbance in erythroid maturation process, the proliferation in the erythroid cells could cause an increase in RDW.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"8 1","pages":"93-98"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81668998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Somi, E. Faramarzi, Shanaz Nagashi, Jalil Amirifar
Objective: Take in to account the relationship between obesity and many diseases and con- tradictory published results considering the effects of H. pylori infection on leptin and ghre- lin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was con- firmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were deter- mined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically signifi- cant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.
{"title":"Does Helicobacter pylori eradication effect body composition, dietary intake, serum leptin and ghrelin levels of infected patients?","authors":"M. Somi, E. Faramarzi, Shanaz Nagashi, Jalil Amirifar","doi":"10.5505/TJB.2014.71463","DOIUrl":"https://doi.org/10.5505/TJB.2014.71463","url":null,"abstract":"Objective: Take in to account the relationship between obesity and many diseases and con- tradictory published results considering the effects of H. pylori infection on leptin and ghre- lin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was con- firmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were deter- mined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically signifi- cant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"95 1","pages":"196-200"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80390573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Structural conversion of normal cellular prion protein (PrPc) into the scrapie isoform (PrPsc) is the central event in the development of prion diseases. Materials and Methods: To get more insight into the molecular basis of the stability of animal prion protein, 10 ns molecular dynamics (MD) and flow molecular dynamics (FMD) simulations of turtle prion protein (tPrPc) and bank vole prion protein (bvPrPc) have been performed in this paper. Results: The dynamics and mechanical properties of the two model proteins have been stud- ied. Conclusion: Various motions of β-sheet appeared in the two proteins, such as twisting, elon- gation and unfolding. For α-helix, it is more readily to unfold in bvPrPc system. Furthermore, the protective wall staggered with helix is found to be strong enough to stabilize PrPc under the shear flow.
{"title":"Dynamics study on the stability of animal prion proteins","authors":"Xin Chen, Danhui Duan, Shuyan Zhu, Yafang Liu","doi":"10.5505/TJB.2014.21033","DOIUrl":"https://doi.org/10.5505/TJB.2014.21033","url":null,"abstract":"Aim: Structural conversion of normal cellular prion protein (PrPc) into the scrapie isoform (PrPsc) is the central event in the development of prion diseases. Materials and Methods: To get more insight into the molecular basis of the stability of animal prion protein, 10 ns molecular dynamics (MD) and flow molecular dynamics (FMD) simulations of turtle prion protein (tPrPc) and bank vole prion protein (bvPrPc) have been performed in this paper. Results: The dynamics and mechanical properties of the two model proteins have been stud- ied. Conclusion: Various motions of β-sheet appeared in the two proteins, such as twisting, elon- gation and unfolding. For α-helix, it is more readily to unfold in bvPrPc system. Furthermore, the protective wall staggered with helix is found to be strong enough to stabilize PrPc under the shear flow.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"22 1","pages":"188-195"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79029882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Within the context of good clinical laboratory practices evaluation of measurement uncertainty of Thyroid Stimulating Hormone and Prostate Specific Antigen parameters","authors":"S. Yildirmak","doi":"10.5505/tjb.2014.49358","DOIUrl":"https://doi.org/10.5505/tjb.2014.49358","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"10 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72634278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Bal, M. Serdar, O. T. Güngör, H. Celik, S. Abuşoğlu, N. Uğuz, G. Erden, M. Yıldırımkaya
Objective: The aim of this study is the calculation of measurement uncertainty values of ten different biochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline / 12 /, European Technical Report: 1 / 3 / and ISO / DTS 21748 Guideline / 8 / was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it.
{"title":"Calculation of measurement uncertainty of biochemical parameters","authors":"C. Bal, M. Serdar, O. T. Güngör, H. Celik, S. Abuşoğlu, N. Uğuz, G. Erden, M. Yıldırımkaya","doi":"10.5505/TJB.2014.04127","DOIUrl":"https://doi.org/10.5505/TJB.2014.04127","url":null,"abstract":"Objective: The aim of this study is the calculation of measurement uncertainty values of ten different biochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline / 12 /, European Technical Report: 1 / 3 / and ISO / DTS 21748 Guideline / 8 / was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"108 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75906240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: High K+ value does not serve just to maintain osmotic balance. It is hypothesized that the relatively high intracellular levels of K+ maintained by most cells functions to furnish other cellular functions such as augmentation of protein levels through either synthesis or metabolism of protein. The study aimed at establishing a correlation between the mineral and protein contents of foods. Methods: The mineral (Na+, K+, Ca2+ and Mg2+) and protein contents of some randomly selected plant liquid products were estimated. Concentration-dependent mineral profiles were drawn up and ratios of the various minerals, protein/ (K+), protein/(Na+) and (K+)/ (Na+) versus protein levels were calculated.
{"title":"Possible correlation between mineral profile and protein content of foods","authors":"C. Ibegbulem, S. Abanobi","doi":"10.5505/TJB.2014.61587","DOIUrl":"https://doi.org/10.5505/TJB.2014.61587","url":null,"abstract":"Objective: High K+ value does not serve just to maintain osmotic balance. It is hypothesized that the relatively high intracellular levels of K+ maintained by most cells functions to furnish other cellular functions such as augmentation of protein levels through either synthesis or metabolism of protein. The study aimed at establishing a correlation between the mineral and protein contents of foods. Methods: The mineral (Na+, K+, Ca2+ and Mg2+) and protein contents of some randomly selected plant liquid products were estimated. Concentration-dependent mineral profiles were drawn up and ratios of the various minerals, protein/ (K+), protein/(Na+) and (K+)/ (Na+) versus protein levels were calculated.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"63 1","pages":"45-50"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74406067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.
{"title":"Association of HLA-B27, MEFV gene mutations, ERAP1, IL12B and IL23R gene polymorphisms with ankylosing spondylitis.","authors":"E. Yılmaz","doi":"10.5505/TJB.2014.44265","DOIUrl":"https://doi.org/10.5505/TJB.2014.44265","url":null,"abstract":"Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"50 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73377869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umumicroplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-Dgalactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn’t caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate.
目的:首次采用umu-微孔板检测系统对6种设计为药物的硝基罗布烷(I)衍生物进行基因毒性检测。药物开发的一个重要原则是在体外试验中对先前确定的显著药物活性进行安全性试验。这可能比它在实验条件下的效率更重要，因为在化疗中对患者无风险的治疗是很重要的。方法:专门设计umu -微孔板检测系统，用于检测硝基化合物的致突变性。1-[(2-氨基苯基)硫]-1-苯基-2-硝基丁烷(I)衍生物含有硝基。因此，我们选择umu-微孔板检测系统进行分析。采用鼠伤寒沙门菌NM1011(过表达硝基还原酶)和鼠伤寒沙门菌NM2009(过表达O-At (o -乙酰基转移酶))对硝基化合物敏感，采用微孔板检测系统评价所检测化合物的SOS诱导活性。以氯酚红-β- d-半乳糖苷(CPRG)和o -硝基苯基-β-双乳糖苷(ONPG)为底物，以4-硝基喹啉- 1-氧化物(4NQO)为阳性对照。结果:虽然使用CPRG的β-半乳糖苷酶活性比使用ONPG的高3倍，但所有化合物对底物和菌株都有相似的结果。对于所有化合物，发现过表达NR和O-At的菌株对umuC基因表达的诱导作用几乎相同。所测试的衍生物对两种菌株的过表达NR和O-At酶没有明显的诱导作用，这两种酶在硝基化合物的代谢激活机制中起作用。结论:我们的研究表明，1-[(2-氨基苯基)硫]-1-苯基-2-硝基丁烷衍生物在该试验系统中无遗传毒性作用。这一结果是一个非常重要的数据，使它们成为潜在的候选药物。
{"title":"Genotoxicity of potent antiviral 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives designed as drug agents","authors":"T. Doğan, M. Durusoy, M. Gokçe, Kamile Öztürk","doi":"10.5505/TJB.2014.25238","DOIUrl":"https://doi.org/10.5505/TJB.2014.25238","url":null,"abstract":"Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umumicroplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-Dgalactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn’t caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"41 1","pages":"328-335"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79739337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ser159 mutatıon of the pre-ınıtıatıon complex proteinTAF7 causes a G2/M block in cell cycle","authors":"Z. Sercan","doi":"10.5505/tjb.2014.49091","DOIUrl":"https://doi.org/10.5505/tjb.2014.49091","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"38 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81771234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts. Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 μmol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms. Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.
{"title":"Antioxidant and antimicrobial activity of East Black Sea Region honeys)","authors":"Ö. Ertürk, H. Sahin, S. Kolaylı, M. C. Ayvaz","doi":"10.5505/TJB.2014.77487","DOIUrl":"https://doi.org/10.5505/TJB.2014.77487","url":null,"abstract":"Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts. Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 μmol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms. Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"397 1","pages":"99-106"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85020345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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