N. Kozaci, F. Gungor, M. Ay, I. Beydilli, Nuri Bozdemir, C. Akyol
Objective: The increase in alveolar ventilation causes disposal of large amounts of carbon dioxide from the respiratory system resulting in hypocapnia and respiratory alkalosis. Hypocapnia reduces cerebral blood flow, alkalosis leads to a leftward shift in oxygen- hemoglobin dissociation curve causing reduced oxygen delivery to tissues. Therefore, immediate diagnosis and close monitoring of respiratory alkalosis is necessary in emergency situations. In this study, the comparison of arterial and venous blood gas parameters of patients with respiratory alkalosis, and the evaluation of the usability of venous blood gas instead of arterial blood gas in patients with respiratory alkalosis were aimed. Methods: Ninety patients with respiratory alkalosis were enrolled in this study prospectively. Arterial and venous blood gas samples of patients enrolled in the study were drawn simultaneously in room air without administering any treatment after admitting to the emergency department. Results: The correlation between the results of pH, partial pressure of carbon dioxide (pCO2), bicarbonate (HCO 3 ) and base excess (BE) (respectively, r= 0.764, r= 0.839, r= 0.843, r= 0.883) in arterial and venous blood gas samples were statistically significant (p 80 mmHg in 23 patients (25.6%), between 60-80 mmHg in 29 (32.2%) patients and <60 mmHg in 38 (42.2%) patients. A statistically significant correlation (r= 0.540, p<0.001) detected only between arterial and venous blood gas samples of patients with PaO 2 <60mmHg and O 2 saturation <90% among these three groups. Conclusion: In the follow-up of patients with respiratory alkalosis, if O2 saturation is ≥90%; pH, pCO 2 and HCO 3 of venous blood gas samples can be used instead of arterial blood gas samples. If patient's O2 saturation <90%; pH, pCO2, HCO3 and also pO2 values in venous blood gas sample can be used instead of arterial blood gas samples.
{"title":"Can venous blood gas values be used instead of arterial blood gas values in respiratory alkalosis","authors":"N. Kozaci, F. Gungor, M. Ay, I. Beydilli, Nuri Bozdemir, C. Akyol","doi":"10.5505/TJB.2014.87049","DOIUrl":"https://doi.org/10.5505/TJB.2014.87049","url":null,"abstract":"Objective: The increase in alveolar ventilation causes disposal of large amounts of carbon dioxide from the respiratory system resulting in hypocapnia and respiratory alkalosis. Hypocapnia reduces cerebral blood flow, alkalosis leads to a leftward shift in oxygen- hemoglobin dissociation curve causing reduced oxygen delivery to tissues. Therefore, immediate diagnosis and close monitoring of respiratory alkalosis is necessary in emergency situations. In this study, the comparison of arterial and venous blood gas parameters of patients with respiratory alkalosis, and the evaluation of the usability of venous blood gas instead of arterial blood gas in patients with respiratory alkalosis were aimed. Methods: Ninety patients with respiratory alkalosis were enrolled in this study prospectively. Arterial and venous blood gas samples of patients enrolled in the study were drawn simultaneously in room air without administering any treatment after admitting to the emergency department. Results: The correlation between the results of pH, partial pressure of carbon dioxide (pCO2), bicarbonate (HCO 3 ) and base excess (BE) (respectively, r= 0.764, r= 0.839, r= 0.843, r= 0.883) in arterial and venous blood gas samples were statistically significant (p 80 mmHg in 23 patients (25.6%), between 60-80 mmHg in 29 (32.2%) patients and <60 mmHg in 38 (42.2%) patients. A statistically significant correlation (r= 0.540, p<0.001) detected only between arterial and venous blood gas samples of patients with PaO 2 <60mmHg and O 2 saturation <90% among these three groups. Conclusion: In the follow-up of patients with respiratory alkalosis, if O2 saturation is ≥90%; pH, pCO 2 and HCO 3 of venous blood gas samples can be used instead of arterial blood gas samples. If patient's O2 saturation <90%; pH, pCO2, HCO3 and also pO2 values in venous blood gas sample can be used instead of arterial blood gas samples.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82638555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Bal, M. Serdar, O. T. Güngör, H. Celik, S. Abuşoğlu, N. Uğuz, G. Erden, M. Yıldırımkaya
Objective: The aim of this study is the calculation of measurement uncertainty values of ten different biochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline / 12 /, European Technical Report: 1 / 3 / and ISO / DTS 21748 Guideline / 8 / was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it.
{"title":"Calculation of measurement uncertainty of biochemical parameters","authors":"C. Bal, M. Serdar, O. T. Güngör, H. Celik, S. Abuşoğlu, N. Uğuz, G. Erden, M. Yıldırımkaya","doi":"10.5505/TJB.2014.04127","DOIUrl":"https://doi.org/10.5505/TJB.2014.04127","url":null,"abstract":"Objective: The aim of this study is the calculation of measurement uncertainty values of ten different biochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline / 12 /, European Technical Report: 1 / 3 / and ISO / DTS 21748 Guideline / 8 / was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75906240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Within the context of good clinical laboratory practices evaluation of measurement uncertainty of Thyroid Stimulating Hormone and Prostate Specific Antigen parameters","authors":"S. Yildirmak","doi":"10.5505/tjb.2014.49358","DOIUrl":"https://doi.org/10.5505/tjb.2014.49358","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72634278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: High K+ value does not serve just to maintain osmotic balance. It is hypothesized that the relatively high intracellular levels of K+ maintained by most cells functions to furnish other cellular functions such as augmentation of protein levels through either synthesis or metabolism of protein. The study aimed at establishing a correlation between the mineral and protein contents of foods. Methods: The mineral (Na+, K+, Ca2+ and Mg2+) and protein contents of some randomly selected plant liquid products were estimated. Concentration-dependent mineral profiles were drawn up and ratios of the various minerals, protein/ (K+), protein/(Na+) and (K+)/ (Na+) versus protein levels were calculated.
{"title":"Possible correlation between mineral profile and protein content of foods","authors":"C. Ibegbulem, S. Abanobi","doi":"10.5505/TJB.2014.61587","DOIUrl":"https://doi.org/10.5505/TJB.2014.61587","url":null,"abstract":"Objective: High K+ value does not serve just to maintain osmotic balance. It is hypothesized that the relatively high intracellular levels of K+ maintained by most cells functions to furnish other cellular functions such as augmentation of protein levels through either synthesis or metabolism of protein. The study aimed at establishing a correlation between the mineral and protein contents of foods. Methods: The mineral (Na+, K+, Ca2+ and Mg2+) and protein contents of some randomly selected plant liquid products were estimated. Concentration-dependent mineral profiles were drawn up and ratios of the various minerals, protein/ (K+), protein/(Na+) and (K+)/ (Na+) versus protein levels were calculated.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74406067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Structural conversion of normal cellular prion protein (PrPc) into the scrapie isoform (PrPsc) is the central event in the development of prion diseases. Materials and Methods: To get more insight into the molecular basis of the stability of animal prion protein, 10 ns molecular dynamics (MD) and flow molecular dynamics (FMD) simulations of turtle prion protein (tPrPc) and bank vole prion protein (bvPrPc) have been performed in this paper. Results: The dynamics and mechanical properties of the two model proteins have been stud- ied. Conclusion: Various motions of β-sheet appeared in the two proteins, such as twisting, elon- gation and unfolding. For α-helix, it is more readily to unfold in bvPrPc system. Furthermore, the protective wall staggered with helix is found to be strong enough to stabilize PrPc under the shear flow.
{"title":"Dynamics study on the stability of animal prion proteins","authors":"Xin Chen, Danhui Duan, Shuyan Zhu, Yafang Liu","doi":"10.5505/TJB.2014.21033","DOIUrl":"https://doi.org/10.5505/TJB.2014.21033","url":null,"abstract":"Aim: Structural conversion of normal cellular prion protein (PrPc) into the scrapie isoform (PrPsc) is the central event in the development of prion diseases. Materials and Methods: To get more insight into the molecular basis of the stability of animal prion protein, 10 ns molecular dynamics (MD) and flow molecular dynamics (FMD) simulations of turtle prion protein (tPrPc) and bank vole prion protein (bvPrPc) have been performed in this paper. Results: The dynamics and mechanical properties of the two model proteins have been stud- ied. Conclusion: Various motions of β-sheet appeared in the two proteins, such as twisting, elon- gation and unfolding. For α-helix, it is more readily to unfold in bvPrPc system. Furthermore, the protective wall staggered with helix is found to be strong enough to stabilize PrPc under the shear flow.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79029882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Somi, E. Faramarzi, Shanaz Nagashi, Jalil Amirifar
Objective: Take in to account the relationship between obesity and many diseases and con- tradictory published results considering the effects of H. pylori infection on leptin and ghre- lin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was con- firmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were deter- mined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically signifi- cant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.
{"title":"Does Helicobacter pylori eradication effect body composition, dietary intake, serum leptin and ghrelin levels of infected patients?","authors":"M. Somi, E. Faramarzi, Shanaz Nagashi, Jalil Amirifar","doi":"10.5505/TJB.2014.71463","DOIUrl":"https://doi.org/10.5505/TJB.2014.71463","url":null,"abstract":"Objective: Take in to account the relationship between obesity and many diseases and con- tradictory published results considering the effects of H. pylori infection on leptin and ghre- lin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was con- firmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were deter- mined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically signifi- cant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80390573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ser159 mutatıon of the pre-ınıtıatıon complex proteinTAF7 causes a G2/M block in cell cycle","authors":"Z. Sercan","doi":"10.5505/tjb.2014.49091","DOIUrl":"https://doi.org/10.5505/tjb.2014.49091","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81771234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hb South Florida is a rare hemoglobin beta chain variant that is not associated with any clinical disorder. We report a heterozygous Hb South Florida (β1(NA1)Val>Met, GTG>ATG; HBB:c.4G>A) case determined during a premarital screening program. This hemoglobin variant can be identified with high performance liquid chromatography analysis confirmed with DNA sequencing. We emphasize in our study the importance of an interdisciplinary collaborative study at the provincial basis for the success of the hemoglobinopathy control program.
南佛罗里达Hb是一种罕见的血红蛋白β链变异，与任何临床疾病无关。我们报道了一个杂合Hb South Florida (β1(NA1)Val>Met, GTG>ATG;HBB:c.4G>A)在婚前筛查项目中确定的病例。这种血红蛋白变体可以通过高效液相色谱分析和DNA测序确认。在我们的研究中，我们强调了跨学科合作研究在省级基础上对血红蛋白病控制项目成功的重要性。
{"title":"A Rare Hemoglobin Variant Which Interfered Hemoglobin A1C Result: Hemoglobin South Florida (β1(NA1)Val>Met, GTG>ATG; HBB: c.4G>A)","authors":"A. Celebiler, D. Güleç, N. Uzuncan","doi":"10.5505/TJB.2014.27247","DOIUrl":"https://doi.org/10.5505/TJB.2014.27247","url":null,"abstract":"Hb South Florida is a rare hemoglobin beta chain variant that is not associated with any clinical disorder. We report a heterozygous Hb South Florida (β1(NA1)Val>Met, GTG>ATG; HBB:c.4G>A) case determined during a premarital screening program. This hemoglobin variant can be identified with high performance liquid chromatography analysis confirmed with DNA sequencing. We emphasize in our study the importance of an interdisciplinary collaborative study at the provincial basis for the success of the hemoglobinopathy control program.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87524889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts. Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 μmol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms. Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.
{"title":"Antioxidant and antimicrobial activity of East Black Sea Region honeys)","authors":"Ö. Ertürk, H. Sahin, S. Kolaylı, M. C. Ayvaz","doi":"10.5505/TJB.2014.77487","DOIUrl":"https://doi.org/10.5505/TJB.2014.77487","url":null,"abstract":"Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts. Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 μmol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms. Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85020345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umumicroplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-Dgalactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn’t caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate.
目的:首次采用umu-微孔板检测系统对6种设计为药物的硝基罗布烷(I)衍生物进行基因毒性检测。药物开发的一个重要原则是在体外试验中对先前确定的显著药物活性进行安全性试验。这可能比它在实验条件下的效率更重要，因为在化疗中对患者无风险的治疗是很重要的。方法:专门设计umu -微孔板检测系统，用于检测硝基化合物的致突变性。1-[(2-氨基苯基)硫]-1-苯基-2-硝基丁烷(I)衍生物含有硝基。因此，我们选择umu-微孔板检测系统进行分析。采用鼠伤寒沙门菌NM1011(过表达硝基还原酶)和鼠伤寒沙门菌NM2009(过表达O-At (o -乙酰基转移酶))对硝基化合物敏感，采用微孔板检测系统评价所检测化合物的SOS诱导活性。以氯酚红-β- d-半乳糖苷(CPRG)和o -硝基苯基-β-双乳糖苷(ONPG)为底物，以4-硝基喹啉- 1-氧化物(4NQO)为阳性对照。结果:虽然使用CPRG的β-半乳糖苷酶活性比使用ONPG的高3倍，但所有化合物对底物和菌株都有相似的结果。对于所有化合物，发现过表达NR和O-At的菌株对umuC基因表达的诱导作用几乎相同。所测试的衍生物对两种菌株的过表达NR和O-At酶没有明显的诱导作用，这两种酶在硝基化合物的代谢激活机制中起作用。结论:我们的研究表明，1-[(2-氨基苯基)硫]-1-苯基-2-硝基丁烷衍生物在该试验系统中无遗传毒性作用。这一结果是一个非常重要的数据，使它们成为潜在的候选药物。
{"title":"Genotoxicity of potent antiviral 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives designed as drug agents","authors":"T. Doğan, M. Durusoy, M. Gokçe, Kamile Öztürk","doi":"10.5505/TJB.2014.25238","DOIUrl":"https://doi.org/10.5505/TJB.2014.25238","url":null,"abstract":"Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umumicroplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-Dgalactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn’t caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79739337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：