Objective: V617F mutation of Janus kinase (JAK2) gene is used in the diagnosis of BCR-ABL negative myeloproliferative diseases such as polycytemia vera, essential thrombocythemia and primary myelofibrosis. In this study, we evaluated the frequency of JAK2 V617F mutation in a group of Turkish individuals and its association with whole blood count parameters. Methods: We retrospectively reviewed the records of 201 patients (174 males and 27 females) who were tested for JAK2 V617F mutation from January 2012 to December 2012. JAK2V617F mutation, BCR-ABL mutation, level of white blood cell count (WBC), red blood cell count (RBC), platelet count (PLT), red blood cell distribution width (RDW), hemoglobin (Hb), hematocrit (Hct), level of iron, iron binding capacity and demographic data of patients were also noted. A commercial kit assay for the identification of V617F mutation based on real time-polymerase chain reaction had been used to investigate mutation of the JAK2 gene. Results: 14.9 % of the 201 samples being investigated for JAK2 V617F mutation were positive. Patients who were ordered for JAK2 V617F mutation testing were divided into two groups according to mutation being positive and negative. Age, RDW and PLT levels were significantly higher in mutation positive group (p<0.05). Besides JAK2 V617F mutation, BCR-ABL mutation orders were examined and BCR-ABL mutation reports were also evaluated on subjects. Age, RDW and PLT were significantly higher in JAK2 V617F mutation positive / BCR-ABL mutation negative group (p<0.05). Conclusion: In this study significant differences for age, RDW and platelet count were determined in mutation positive subjects. Findings of this study suggest that JAK2 V617F mutation could cause proliferation of megakaryocytic cells as well as erythroid cells and as a result of disturbance in erythroid maturation process, the proliferation in the erythroid cells could cause an increase in RDW.
{"title":"The Frequency Of JAK2 V617F Gen Mutation And Its Associations With Whole Blood Count Parameters","authors":"Kübranur Ünal, S. Erdogan, F. Yilmaz","doi":"10.5505/TJB.2014.39206","DOIUrl":"https://doi.org/10.5505/TJB.2014.39206","url":null,"abstract":"Objective: V617F mutation of Janus kinase (JAK2) gene is used in the diagnosis of BCR-ABL negative myeloproliferative diseases such as polycytemia vera, essential thrombocythemia and primary myelofibrosis. In this study, we evaluated the frequency of JAK2 V617F mutation in a group of Turkish individuals and its association with whole blood count parameters. Methods: We retrospectively reviewed the records of 201 patients (174 males and 27 females) who were tested for JAK2 V617F mutation from January 2012 to December 2012. JAK2V617F mutation, BCR-ABL mutation, level of white blood cell count (WBC), red blood cell count (RBC), platelet count (PLT), red blood cell distribution width (RDW), hemoglobin (Hb), hematocrit (Hct), level of iron, iron binding capacity and demographic data of patients were also noted. A commercial kit assay for the identification of V617F mutation based on real time-polymerase chain reaction had been used to investigate mutation of the JAK2 gene. Results: 14.9 % of the 201 samples being investigated for JAK2 V617F mutation were positive. Patients who were ordered for JAK2 V617F mutation testing were divided into two groups according to mutation being positive and negative. Age, RDW and PLT levels were significantly higher in mutation positive group (p<0.05). Besides JAK2 V617F mutation, BCR-ABL mutation orders were examined and BCR-ABL mutation reports were also evaluated on subjects. Age, RDW and PLT were significantly higher in JAK2 V617F mutation positive / BCR-ABL mutation negative group (p<0.05). Conclusion: In this study significant differences for age, RDW and platelet count were determined in mutation positive subjects. Findings of this study suggest that JAK2 V617F mutation could cause proliferation of megakaryocytic cells as well as erythroid cells and as a result of disturbance in erythroid maturation process, the proliferation in the erythroid cells could cause an increase in RDW.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"8 1","pages":"93-98"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81668998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Within the context of good clinical laboratory practices evaluation of measurement uncertainty of Thyroid Stimulating Hormone and Prostate Specific Antigen parameters","authors":"S. Yildirmak","doi":"10.5505/tjb.2014.49358","DOIUrl":"https://doi.org/10.5505/tjb.2014.49358","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"10 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72634278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Bal, M. Serdar, O. T. Güngör, H. Celik, S. Abuşoğlu, N. Uğuz, G. Erden, M. Yıldırımkaya
Objective: The aim of this study is the calculation of measurement uncertainty values of ten different biochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline / 12 /, European Technical Report: 1 / 3 / and ISO / DTS 21748 Guideline / 8 / was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it.
{"title":"Calculation of measurement uncertainty of biochemical parameters","authors":"C. Bal, M. Serdar, O. T. Güngör, H. Celik, S. Abuşoğlu, N. Uğuz, G. Erden, M. Yıldırımkaya","doi":"10.5505/TJB.2014.04127","DOIUrl":"https://doi.org/10.5505/TJB.2014.04127","url":null,"abstract":"Objective: The aim of this study is the calculation of measurement uncertainty values of ten different biochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline / 12 /, European Technical Report: 1 / 3 / and ISO / DTS 21748 Guideline / 8 / was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"108 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75906240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: High K+ value does not serve just to maintain osmotic balance. It is hypothesized that the relatively high intracellular levels of K+ maintained by most cells functions to furnish other cellular functions such as augmentation of protein levels through either synthesis or metabolism of protein. The study aimed at establishing a correlation between the mineral and protein contents of foods. Methods: The mineral (Na+, K+, Ca2+ and Mg2+) and protein contents of some randomly selected plant liquid products were estimated. Concentration-dependent mineral profiles were drawn up and ratios of the various minerals, protein/ (K+), protein/(Na+) and (K+)/ (Na+) versus protein levels were calculated.
{"title":"Possible correlation between mineral profile and protein content of foods","authors":"C. Ibegbulem, S. Abanobi","doi":"10.5505/TJB.2014.61587","DOIUrl":"https://doi.org/10.5505/TJB.2014.61587","url":null,"abstract":"Objective: High K+ value does not serve just to maintain osmotic balance. It is hypothesized that the relatively high intracellular levels of K+ maintained by most cells functions to furnish other cellular functions such as augmentation of protein levels through either synthesis or metabolism of protein. The study aimed at establishing a correlation between the mineral and protein contents of foods. Methods: The mineral (Na+, K+, Ca2+ and Mg2+) and protein contents of some randomly selected plant liquid products were estimated. Concentration-dependent mineral profiles were drawn up and ratios of the various minerals, protein/ (K+), protein/(Na+) and (K+)/ (Na+) versus protein levels were calculated.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"63 1","pages":"45-50"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74406067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Structural conversion of normal cellular prion protein (PrPc) into the scrapie isoform (PrPsc) is the central event in the development of prion diseases. Materials and Methods: To get more insight into the molecular basis of the stability of animal prion protein, 10 ns molecular dynamics (MD) and flow molecular dynamics (FMD) simulations of turtle prion protein (tPrPc) and bank vole prion protein (bvPrPc) have been performed in this paper. Results: The dynamics and mechanical properties of the two model proteins have been stud- ied. Conclusion: Various motions of β-sheet appeared in the two proteins, such as twisting, elon- gation and unfolding. For α-helix, it is more readily to unfold in bvPrPc system. Furthermore, the protective wall staggered with helix is found to be strong enough to stabilize PrPc under the shear flow.
{"title":"Dynamics study on the stability of animal prion proteins","authors":"Xin Chen, Danhui Duan, Shuyan Zhu, Yafang Liu","doi":"10.5505/TJB.2014.21033","DOIUrl":"https://doi.org/10.5505/TJB.2014.21033","url":null,"abstract":"Aim: Structural conversion of normal cellular prion protein (PrPc) into the scrapie isoform (PrPsc) is the central event in the development of prion diseases. Materials and Methods: To get more insight into the molecular basis of the stability of animal prion protein, 10 ns molecular dynamics (MD) and flow molecular dynamics (FMD) simulations of turtle prion protein (tPrPc) and bank vole prion protein (bvPrPc) have been performed in this paper. Results: The dynamics and mechanical properties of the two model proteins have been stud- ied. Conclusion: Various motions of β-sheet appeared in the two proteins, such as twisting, elon- gation and unfolding. For α-helix, it is more readily to unfold in bvPrPc system. Furthermore, the protective wall staggered with helix is found to be strong enough to stabilize PrPc under the shear flow.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"22 1","pages":"188-195"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79029882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Somi, E. Faramarzi, Shanaz Nagashi, Jalil Amirifar
Objective: Take in to account the relationship between obesity and many diseases and con- tradictory published results considering the effects of H. pylori infection on leptin and ghre- lin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was con- firmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were deter- mined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically signifi- cant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.
{"title":"Does Helicobacter pylori eradication effect body composition, dietary intake, serum leptin and ghrelin levels of infected patients?","authors":"M. Somi, E. Faramarzi, Shanaz Nagashi, Jalil Amirifar","doi":"10.5505/TJB.2014.71463","DOIUrl":"https://doi.org/10.5505/TJB.2014.71463","url":null,"abstract":"Objective: Take in to account the relationship between obesity and many diseases and con- tradictory published results considering the effects of H. pylori infection on leptin and ghre- lin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was con- firmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were deter- mined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically signifi- cant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"95 1","pages":"196-200"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80390573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and opti- mize the growth conditions for maximum production of phytase. Material and Methods: The screening of isolates was performed on phytase screening me- dium. The best producer was selected. Phytase activity was determined by measur ing the amount of liberated inorganic phosphate. Optimal culture conditions and fermentation pa- rameters for phytase production were assessed. Results: 236 Bacillus sp. strains isolated. The best phytase producing strain showed higher enzyme yield in the presence of wheat bran and lactose as carbon source, meat extract as organic nitrogen source, CaCl 2 as metal source. 0.3% as phytate concentration was found to be best. In the physical parameters, the best results was obtained at 35°C, pH 7.5, 200 rpm as agitation rate, 2-4% as inoculum size and 48 h as inoculum age. A new medium was obtained by optimizing the incubation conditions of phytase production from Bacillus sp. EBD 9-1. In this medium, enzyme yield was enhanced 62% compared to basal medium. Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive.
{"title":"Screening of phytate hydrolysis Bacillus sp. isolated from soil and optimization of the certain nutritional and physical parameters on the production of phytase","authors":"E. Demirkan, E. Baygin, A. Usta","doi":"10.5505/TJB.2014.26817","DOIUrl":"https://doi.org/10.5505/TJB.2014.26817","url":null,"abstract":"Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and opti- mize the growth conditions for maximum production of phytase. Material and Methods: The screening of isolates was performed on phytase screening me- dium. The best producer was selected. Phytase activity was determined by measur ing the amount of liberated inorganic phosphate. Optimal culture conditions and fermentation pa- rameters for phytase production were assessed. Results: 236 Bacillus sp. strains isolated. The best phytase producing strain showed higher enzyme yield in the presence of wheat bran and lactose as carbon source, meat extract as organic nitrogen source, CaCl 2 as metal source. 0.3% as phytate concentration was found to be best. In the physical parameters, the best results was obtained at 35°C, pH 7.5, 200 rpm as agitation rate, 2-4% as inoculum size and 48 h as inoculum age. A new medium was obtained by optimizing the incubation conditions of phytase production from Bacillus sp. EBD 9-1. In this medium, enzyme yield was enhanced 62% compared to basal medium. Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"47 1","pages":"206-214"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90050693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umumicroplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-Dgalactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn’t caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate.
目的:首次采用umu-微孔板检测系统对6种设计为药物的硝基罗布烷(I)衍生物进行基因毒性检测。药物开发的一个重要原则是在体外试验中对先前确定的显著药物活性进行安全性试验。这可能比它在实验条件下的效率更重要，因为在化疗中对患者无风险的治疗是很重要的。方法:专门设计umu -微孔板检测系统，用于检测硝基化合物的致突变性。1-[(2-氨基苯基)硫]-1-苯基-2-硝基丁烷(I)衍生物含有硝基。因此，我们选择umu-微孔板检测系统进行分析。采用鼠伤寒沙门菌NM1011(过表达硝基还原酶)和鼠伤寒沙门菌NM2009(过表达O-At (o -乙酰基转移酶))对硝基化合物敏感，采用微孔板检测系统评价所检测化合物的SOS诱导活性。以氯酚红-β- d-半乳糖苷(CPRG)和o -硝基苯基-β-双乳糖苷(ONPG)为底物，以4-硝基喹啉- 1-氧化物(4NQO)为阳性对照。结果:虽然使用CPRG的β-半乳糖苷酶活性比使用ONPG的高3倍，但所有化合物对底物和菌株都有相似的结果。对于所有化合物，发现过表达NR和O-At的菌株对umuC基因表达的诱导作用几乎相同。所测试的衍生物对两种菌株的过表达NR和O-At酶没有明显的诱导作用，这两种酶在硝基化合物的代谢激活机制中起作用。结论:我们的研究表明，1-[(2-氨基苯基)硫]-1-苯基-2-硝基丁烷衍生物在该试验系统中无遗传毒性作用。这一结果是一个非常重要的数据，使它们成为潜在的候选药物。
{"title":"Genotoxicity of potent antiviral 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives designed as drug agents","authors":"T. Doğan, M. Durusoy, M. Gokçe, Kamile Öztürk","doi":"10.5505/TJB.2014.25238","DOIUrl":"https://doi.org/10.5505/TJB.2014.25238","url":null,"abstract":"Objective: Genotoxic potentials of six selected nitrobutane (I) derivatives designed as drug agents were tested here for the first time using umu-microplate test system. An important principle in drug development is to perform safety tests of previously determined significant drug activity in in vitro assays. This may be even more crucial than its efficiency in terms of experimental conditions, since it is important in chemotherapy to treat without risk for the patient. Methods: Umu-microplate test system is especially designed for detecting the mutagenicity of nitro compounds. 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (I) derivatives involve nitro groups. Therefore umu-microplate test system has been chosen for our analysis. Evaluation of the SOS inducing activity of the tested compounds was examined with the umumicroplate test system using Salmonella typhimurium NM1011 (overexpressed NR (nitroreductase)) and S.typhimurium NM2009 (overexpressed O-At (O-acethyltransferase))strains which are sensitive to nitro compounds. Chlorophenol red-β-D-galactopyranoside (CPRG) and O-nitrophenyl-β-Dgalactopyranoside (ONPG) were used as substrate in the enzyme assays and also the well-known genotoxic nitro compound, 4-nitroquinoline 1-oxide (4NQO), was the positive control in the test. Results: Although the β-galactosidase activities with using CPRG were three fold higher than ONPG, parallel results were obtained for both substrates and strains with all compounds tested. For all compounds, the induction of umuC gene expression was found to be almost the same for the strains that overexpress NR and O-At. The derivatives tested didn’t caused an evident induction in both strains overexpressed NR and O-At enzymes which have a role in metabolic activation mechanism of nitro compunds. Conclusion: Our study showed that, 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane derivatives have no genotoxic effects in this test system. This result is a very important data making them a potential drug candidate.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"41 1","pages":"328-335"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79739337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hb South Florida is a rare hemoglobin beta chain variant that is not associated with any clinical disorder. We report a heterozygous Hb South Florida (β1(NA1)Val>Met, GTG>ATG; HBB:c.4G>A) case determined during a premarital screening program. This hemoglobin variant can be identified with high performance liquid chromatography analysis confirmed with DNA sequencing. We emphasize in our study the importance of an interdisciplinary collaborative study at the provincial basis for the success of the hemoglobinopathy control program.
南佛罗里达Hb是一种罕见的血红蛋白β链变异，与任何临床疾病无关。我们报道了一个杂合Hb South Florida (β1(NA1)Val>Met, GTG>ATG;HBB:c.4G>A)在婚前筛查项目中确定的病例。这种血红蛋白变体可以通过高效液相色谱分析和DNA测序确认。在我们的研究中，我们强调了跨学科合作研究在省级基础上对血红蛋白病控制项目成功的重要性。
{"title":"A Rare Hemoglobin Variant Which Interfered Hemoglobin A1C Result: Hemoglobin South Florida (β1(NA1)Val>Met, GTG>ATG; HBB: c.4G>A)","authors":"A. Celebiler, D. Güleç, N. Uzuncan","doi":"10.5505/TJB.2014.27247","DOIUrl":"https://doi.org/10.5505/TJB.2014.27247","url":null,"abstract":"Hb South Florida is a rare hemoglobin beta chain variant that is not associated with any clinical disorder. We report a heterozygous Hb South Florida (β1(NA1)Val>Met, GTG>ATG; HBB:c.4G>A) case determined during a premarital screening program. This hemoglobin variant can be identified with high performance liquid chromatography analysis confirmed with DNA sequencing. We emphasize in our study the importance of an interdisciplinary collaborative study at the provincial basis for the success of the hemoglobinopathy control program.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"16 1","pages":"226-230"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87524889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ser159 mutatıon of the pre-ınıtıatıon complex proteinTAF7 causes a G2/M block in cell cycle","authors":"Z. Sercan","doi":"10.5505/tjb.2014.49091","DOIUrl":"https://doi.org/10.5505/tjb.2014.49091","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"38 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81771234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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