Objective: Intestinal fatty acid binding protein (IFAPB) participates in the uptake, intracellular metabolism and/or transport of long chain fatty acids. A polymorphism at codon 54 in exon 2 of FABP2 gene, which encodes for the IFAPB, exchanges an Alanine for Threonine. FABP2 gene polymorphism could modify the uptake of fatty acids, and it could correlate with risk of several diseases. In the light of the potential role of the FABP2 polymorphism, the aim of this study was to determine the frequency of the Ala54Thr FABP2 polymorphism in two Middle Eastern Arab Populations. Materials and Methods: Genotyping was investigated in 182 and 120 unrelated healthy subjects from Bahrain and Jordan, respectively. A PCR-RFLP assay was applied for determination of Ala54Thr (rs1799883) FABP2 polymorphism. Allele frequencies were calculated by direct counting. Hardy Weinberg Equilibrium was evaluated using a Chi- square goodness of fit test. Results: In the studied Bahraini subjects, 52.8% were homozygous for the Ala54/Ala54 genotype, 35.7% were heterozygous for the Ala54/Thr54 genotype and 11.5% were homozygous for the Thr54/Thr54 genotype. The gene frequencies obtained in Jordanians were: 48.3%, 43.3% and 8.4% for Ala54/Ala54; Ala54/Thr54 and Thr54/Thr54 genotypes, respectively. The frequencies of the allele Ala54 and the allele Thr54 of the FABP2 gene were found to be 0.706 and 0.294 for Bahrainis and 0.700 and 0.300 for Jordanians. These results revealed a similar population polymorphism frequency as in previous European and Arab populations' studies. Conclusion: This is the first study to investigate the population frequency of the Thr54 allele in Bahraini and Jordanian populations.
{"title":"Genotype and allele frequencies of the intestinal fatty acid binding protein-2 gene in two Arab populations","authors":"A. Salem","doi":"10.5505/TJB.2014.15870","DOIUrl":"https://doi.org/10.5505/TJB.2014.15870","url":null,"abstract":"Objective: Intestinal fatty acid binding protein (IFAPB) participates in the uptake, intracellular metabolism and/or transport of long chain fatty acids. A polymorphism at codon 54 in exon 2 of FABP2 gene, which encodes for the IFAPB, exchanges an Alanine for Threonine. FABP2 gene polymorphism could modify the uptake of fatty acids, and it could correlate with risk of several diseases. In the light of the potential role of the FABP2 polymorphism, the aim of this study was to determine the frequency of the Ala54Thr FABP2 polymorphism in two Middle Eastern Arab Populations. Materials and Methods: Genotyping was investigated in 182 and 120 unrelated healthy subjects from Bahrain and Jordan, respectively. A PCR-RFLP assay was applied for determination of Ala54Thr (rs1799883) FABP2 polymorphism. Allele frequencies were calculated by direct counting. Hardy Weinberg Equilibrium was evaluated using a Chi- square goodness of fit test. Results: In the studied Bahraini subjects, 52.8% were homozygous for the Ala54/Ala54 genotype, 35.7% were heterozygous for the Ala54/Thr54 genotype and 11.5% were homozygous for the Thr54/Thr54 genotype. The gene frequencies obtained in Jordanians were: 48.3%, 43.3% and 8.4% for Ala54/Ala54; Ala54/Thr54 and Thr54/Thr54 genotypes, respectively. The frequencies of the allele Ala54 and the allele Thr54 of the FABP2 gene were found to be 0.706 and 0.294 for Bahrainis and 0.700 and 0.300 for Jordanians. These results revealed a similar population polymorphism frequency as in previous European and Arab populations' studies. Conclusion: This is the first study to investigate the population frequency of the Thr54 allele in Bahraini and Jordanian populations.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"77 1","pages":"57-62"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74355433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, we aimed to contribute prenatal diagnosis in the Çukurova region having high prevalence of hemoglobinopathies by implementing DNA sequencing analysis for the mutations undetectable by conventional methods. Methods: Hematological parameters of two families applied to Cukurova University Prenatal Diagnosis Center were analyzed. Hb variants were screened by cellulose acetate electrophoresis and by HPLC. DNA samples of cases were isolated using automatic DNA extraction machine. Microarray, RFLP, ARMS and Gap-PCR molecular methodologies carried out for determinations of hemoglobin mutations. Unidentified mutations were resolved by means of DNA Sequence Analysis. Results: We identified a previously unreported homozygous 5 nt deletional mutation [α2 IVS-1 134.-138. nt (TGAGG)] in Turkey by DNA Sequence Analysis. When we investigated twelve cases of this family by Gap-PCR and DNA Sequence Analysis it is observed that seven cases were heterozygous for 5nt deletion, a case found to be heterozygous 20.5 kb deletional mutation and four cases were found to be having no mutation. In the other family applied for prenatal diagnosis was observed to having Hb Stanleyville II [α2 78 (EF 7) Asn→ Lys (AAC→AAA)] mutation which is also a novel mutaion in Turkey. Mutations of eleven members of this family were determined by ARMS, RFLP, Gap-PCR and DNA Sequence Analysis, resulting in two cases heterozygous Hb Stanleyville II, five cases heterozygous 3.7 kb deletion, a case with heterozygous IVS-1-110 (G→C) mutation on β globin gene and three cases do not have any mutation. Conclusion: In this study, two types of mutations observed for the first time in Turkey have been identified.
{"title":"The first observation of two alpha globin mutations in Turkey: Hb Stanleyville II and a homozygous 5nt deletion","authors":"Figen Guzelgul","doi":"10.5505/TJB.2014.31967","DOIUrl":"https://doi.org/10.5505/TJB.2014.31967","url":null,"abstract":"Objective: In this study, we aimed to contribute prenatal diagnosis in the Çukurova region having high prevalence of hemoglobinopathies by implementing DNA sequencing analysis for the mutations undetectable by conventional methods. Methods: Hematological parameters of two families applied to Cukurova University Prenatal Diagnosis Center were analyzed. Hb variants were screened by cellulose acetate electrophoresis and by HPLC. DNA samples of cases were isolated using automatic DNA extraction machine. Microarray, RFLP, ARMS and Gap-PCR molecular methodologies carried out for determinations of hemoglobin mutations. Unidentified mutations were resolved by means of DNA Sequence Analysis. Results: We identified a previously unreported homozygous 5 nt deletional mutation [α2 IVS-1 134.-138. nt (TGAGG)] in Turkey by DNA Sequence Analysis. When we investigated twelve cases of this family by Gap-PCR and DNA Sequence Analysis it is observed that seven cases were heterozygous for 5nt deletion, a case found to be heterozygous 20.5 kb deletional mutation and four cases were found to be having no mutation. In the other family applied for prenatal diagnosis was observed to having Hb Stanleyville II [α2 78 (EF 7) Asn→ Lys (AAC→AAA)] mutation which is also a novel mutaion in Turkey. Mutations of eleven members of this family were determined by ARMS, RFLP, Gap-PCR and DNA Sequence Analysis, resulting in two cases heterozygous Hb Stanleyville II, five cases heterozygous 3.7 kb deletion, a case with heterozygous IVS-1-110 (G→C) mutation on β globin gene and three cases do not have any mutation. Conclusion: In this study, two types of mutations observed for the first time in Turkey have been identified.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"56 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76472441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Kural, A. Alver, S. Canpolat, C. Kahraman, D. Altay, Hanife Kara, Buket Akcan
Objective: Obesity is a public health problem, but the struggle against obesity continues to increase every day. Lipin-1 is an adipokine that modulates lipid metabolism and glucose homeostasis. The aims of the current study were to compare the levels of lipin-1 in serum and tissues (liver, lung, heart, pancreas and kidney) in rat fed with low fat diet (LFD), high fat diet (HFD) and N-acetylcysteine (NAC) supplemented HFD, and to determine the relationships of the lipin-1 levels in serum and tissues with the levels of serum glucose and lipid parameters. Methods: 18 male Sprague-Dawley rats were divided into three groups: LFD group (n=6), HFD group (n=6) and the NAC-supplemented HFD (HFD+NAC) group (n=6). The feeding period of the diets was 85 days. The lipin-1 levels in serum and tissues were determined by using Rat ELISA kit. Results: In comparison to serum lipin-1 levels in HFD group were significantly lower, but heart lipin-1 levels were significantly higher (P<0.017). On the other hand, it was determined that serum and tissue lipin-1 levels were not influenced by NAC supplementation. In addition, no other significant difference in lipin-1 levels of the other tissues among the groups was found. Conclusion: This study showed that HDF affected serum and heart lipin-1 levels, while NAC supplementation did not affect on the lipin-1 levels of serum and of the tissues.
{"title":"The Effects of high fat diets with and without N-Acetylcysteine supplementation on the lipin-1 levels of serum and various tissues in rats","authors":"B. Kural, A. Alver, S. Canpolat, C. Kahraman, D. Altay, Hanife Kara, Buket Akcan","doi":"10.5505/TJB.2014.78941","DOIUrl":"https://doi.org/10.5505/TJB.2014.78941","url":null,"abstract":"Objective: Obesity is a public health problem, but the struggle against obesity continues to increase every day. Lipin-1 is an adipokine that modulates lipid metabolism and glucose homeostasis. The aims of the current study were to compare the levels of lipin-1 in serum and tissues (liver, lung, heart, pancreas and kidney) in rat fed with low fat diet (LFD), high fat diet (HFD) and N-acetylcysteine (NAC) supplemented HFD, and to determine the relationships of the lipin-1 levels in serum and tissues with the levels of serum glucose and lipid parameters. Methods: 18 male Sprague-Dawley rats were divided into three groups: LFD group (n=6), HFD group (n=6) and the NAC-supplemented HFD (HFD+NAC) group (n=6). The feeding period of the diets was 85 days. The lipin-1 levels in serum and tissues were determined by using Rat ELISA kit. Results: In comparison to serum lipin-1 levels in HFD group were significantly lower, but heart lipin-1 levels were significantly higher (P<0.017). On the other hand, it was determined that serum and tissue lipin-1 levels were not influenced by NAC supplementation. In addition, no other significant difference in lipin-1 levels of the other tissues among the groups was found. Conclusion: This study showed that HDF affected serum and heart lipin-1 levels, while NAC supplementation did not affect on the lipin-1 levels of serum and of the tissues.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"20 1","pages":"19-24"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82792725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Duman, C. Bakar, Ertan Eşsi̇zoğlu, Funda Kirtay Tutunculer, Elif Demircan
Objectives: The Ischemia Modified Albumin (IMA) test is a myocardial ischemia marker which may have potential use for the diagnosis of Acute Coronary Syndrome. The aim of this study was determine reference values of IMA test on the healthy people over the age of 15 who's admitted to Canakkale Onsekiz Mart University. Methods: This study was performed on 582 people over 15 years old and healthy in appearan- ce who had presented at the Canakkale Onsekiz Mart University Faculty of Medicine Rese- arch and Application Hospital. The year 2011 population of Turkey was taken as the reference in the distribution of the study group according to age group and gender. A survey which queried the demographic features and health problems was administered to the participants of the study and 9 cc venous blood was drawn. IMA levels were measured by using the spect- rophotometric method defined by Bar-Or. The mean, standard deviation, median, minimum, maximum, and 2.5 % - 97.5 % interval were used in order to detect the IMA reference values. Results: A total of 274 males (47.1 %) and 308 women (52.9 %) were studied. The mean IMA value of the examined group was 0.339 ± 0.093, and the median was 0.343 (Min-Max: 0.053- 0.631), while the 2.5 % - 97.5 % percentil values were 0.145 - 0.530. Conclusion: Studies that indicate the reference levels of the IMA for the diagnosis of ische- mic conditions are insufficient. Our study is expected to contribute to closing this gap.
{"title":"The reference values of ischemia modified albumin levels in healthy people admitted to a university hospital","authors":"C. Duman, C. Bakar, Ertan Eşsi̇zoğlu, Funda Kirtay Tutunculer, Elif Demircan","doi":"10.5505/TJB.2014.32932","DOIUrl":"https://doi.org/10.5505/TJB.2014.32932","url":null,"abstract":"Objectives: The Ischemia Modified Albumin (IMA) test is a myocardial ischemia marker which may have potential use for the diagnosis of Acute Coronary Syndrome. The aim of this study was determine reference values of IMA test on the healthy people over the age of 15 who's admitted to Canakkale Onsekiz Mart University. Methods: This study was performed on 582 people over 15 years old and healthy in appearan- ce who had presented at the Canakkale Onsekiz Mart University Faculty of Medicine Rese- arch and Application Hospital. The year 2011 population of Turkey was taken as the reference in the distribution of the study group according to age group and gender. A survey which queried the demographic features and health problems was administered to the participants of the study and 9 cc venous blood was drawn. IMA levels were measured by using the spect- rophotometric method defined by Bar-Or. The mean, standard deviation, median, minimum, maximum, and 2.5 % - 97.5 % interval were used in order to detect the IMA reference values. Results: A total of 274 males (47.1 %) and 308 women (52.9 %) were studied. The mean IMA value of the examined group was 0.339 ± 0.093, and the median was 0.343 (Min-Max: 0.053- 0.631), while the 2.5 % - 97.5 % percentil values were 0.145 - 0.530. Conclusion: Studies that indicate the reference levels of the IMA for the diagnosis of ische- mic conditions are insufficient. Our study is expected to contribute to closing this gap.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"1 1","pages":"132-139"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82829567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: An important predictor for infant survival is birth weight. Normal fetal growth is related to various intrauterine factors. Low birth weight is thought to have relation with oxidative stress which plays an important role in reducing the birth weight. Among the paraoxonase family PON1 protects LDL and HDL from the lipid peroxidation. This is HDL associated enzyme having antioxidant property. We aimed to evaluate the arylesterase and lactonase activity of PON1 in cord blood in relation to birth weight. We hypothesized that cord blood PON1 arylesterase and lactonase activities will be compromised in neonates having low birth weight. Methods: We included 80 neonates born in our hospital irrespective of mode of delivery as 40 cases and 40 controls. PON1 arylesterase and lactonase activity were measured using spectrophotometer. Results: Serum arylesterase activity decreased significantly in low birth weight babies (p<0.05). Linear regression analysis (R=0.595) indicates significant correlation between arylesterase and birth weight. Serum lactonase activity of PON1 also gets reduced in low birth weight babies. Its linear regression analysis showed (R=0.716) suggesting significant correlation between lactonase and birth weight. Conclusion: Reduced PON1 activity can be explained on the basis of ER stress and atherogenic changes in the placental circulation. Ours is the first study in cord blood paraoxonase activities in relation to birth weight. As the sample in our study is cord blood, it is essentially a noninvasive one. Further studies are needed in this direction to assess the effect of the oxidative stress on fetus through cord blood in its long term prospective.
{"title":"Harbingers of neonatal birth weight: The PON1 arylesterase and lactonase activities","authors":"M. Mogarekar, M. Rojekar","doi":"10.5505/TJB.2014.72473","DOIUrl":"https://doi.org/10.5505/TJB.2014.72473","url":null,"abstract":"Objective: An important predictor for infant survival is birth weight. Normal fetal growth is related to various intrauterine factors. Low birth weight is thought to have relation with oxidative stress which plays an important role in reducing the birth weight. Among the paraoxonase family PON1 protects LDL and HDL from the lipid peroxidation. This is HDL associated enzyme having antioxidant property. We aimed to evaluate the arylesterase and lactonase activity of PON1 in cord blood in relation to birth weight. We hypothesized that cord blood PON1 arylesterase and lactonase activities will be compromised in neonates having low birth weight. Methods: We included 80 neonates born in our hospital irrespective of mode of delivery as 40 cases and 40 controls. PON1 arylesterase and lactonase activity were measured using spectrophotometer. Results: Serum arylesterase activity decreased significantly in low birth weight babies (p<0.05). Linear regression analysis (R=0.595) indicates significant correlation between arylesterase and birth weight. Serum lactonase activity of PON1 also gets reduced in low birth weight babies. Its linear regression analysis showed (R=0.716) suggesting significant correlation between lactonase and birth weight. Conclusion: Reduced PON1 activity can be explained on the basis of ER stress and atherogenic changes in the placental circulation. Ours is the first study in cord blood paraoxonase activities in relation to birth weight. As the sample in our study is cord blood, it is essentially a noninvasive one. Further studies are needed in this direction to assess the effect of the oxidative stress on fetus through cord blood in its long term prospective.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"25 1","pages":"25-29"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87123085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Irshad, Z. Anwar, Z. Mahmood, T. Aqil, S. Mehmmod, H. Nawaz
Objective: Agro-industrial residues are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially important enzymes. For industrial use, pectinases can be produced from several agricultural pectin-containing wastes like orange peel. The selection of cheap source benefits the production of industrial important enzymes in term to costs-effective production. Methods: The indigenously produced pectinase was purified to homogeneity level using a combination of ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography. The molecular weight and properties of the purified enzyme were studied following the standard procedures. Results: Under optimized solid state fermentation conditions Trichoderma viridi exhibited superior enzymatic production. On optimization, the culture showed the maximum enzyme yield (325 U/mL) at 30 °C in an orange peel medium having a pH of 5.5 and a substrate concentration of 4% on the 4th day of fermentation of orange peel based medium that was additionally supplemented with glucose and ammonium chloride as an inexpensive carbon and nitrogen supplements in a ratio of 20:1, respectively. A purification fold of 5.59 with specific activity and percent recovery of 97.2 U/mg and 12.96% was achieved respectively. The molecular weight of purified pectinase from T. viridi was 30 kDa evidenced by PAGE analysis. After 6 h incubation the pectinase activity profile showed that the purified enzyme was optimally active and stable at a pH of 5 and at 60°C. Conclusions: The present study concluded that the indigenous strain T. viridi showed incredible potential for pectinase synthesis. The maximum production of pectinase in the presence of a cheaper substrate at low concentrations makes the enzyme useful in industrial sectors, especially for the textile and juice industries.
{"title":"Bio-processing of agro-industrial waste orange peel for induced production of pectinase by Trichoderma viridi; its purification and characterization","authors":"M. Irshad, Z. Anwar, Z. Mahmood, T. Aqil, S. Mehmmod, H. Nawaz","doi":"10.5505/TJB.2014.55707","DOIUrl":"https://doi.org/10.5505/TJB.2014.55707","url":null,"abstract":"Objective: Agro-industrial residues are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially important enzymes. For industrial use, pectinases can be produced from several agricultural pectin-containing wastes like orange peel. The selection of cheap source benefits the production of industrial important enzymes in term to costs-effective production. Methods: The indigenously produced pectinase was purified to homogeneity level using a combination of ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography. The molecular weight and properties of the purified enzyme were studied following the standard procedures. Results: Under optimized solid state fermentation conditions Trichoderma viridi exhibited superior enzymatic production. On optimization, the culture showed the maximum enzyme yield (325 U/mL) at 30 °C in an orange peel medium having a pH of 5.5 and a substrate concentration of 4% on the 4th day of fermentation of orange peel based medium that was additionally supplemented with glucose and ammonium chloride as an inexpensive carbon and nitrogen supplements in a ratio of 20:1, respectively. A purification fold of 5.59 with specific activity and percent recovery of 97.2 U/mg and 12.96% was achieved respectively. The molecular weight of purified pectinase from T. viridi was 30 kDa evidenced by PAGE analysis. After 6 h incubation the pectinase activity profile showed that the purified enzyme was optimally active and stable at a pH of 5 and at 60°C. Conclusions: The present study concluded that the indigenous strain T. viridi showed incredible potential for pectinase synthesis. The maximum production of pectinase in the presence of a cheaper substrate at low concentrations makes the enzyme useful in industrial sectors, especially for the textile and juice industries.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"66 1","pages":"9-18"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89334156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The aim of the study was to investigate the changes of oxidative and anti-oxidative systems in the splanchnic area during carbon dioxide pneumoperitoneum and to determine whether the administration of dexmedetomidine has effects on these systems. Methods: Forty rats were randomized into four groups: Group I; Control, Group II; No pneumoperitoneum, Dexmedetomidine administration, Group III; Pneumoperitoneum, no Dexmedetomidine administration and Group IV; Pneumoperitoneum and Dexmedetomidine administration 30 minutes before insufflation. The rats were rested 30 minutes after desufflation and blood samples were obtained for; ischaemia modified albumin (IMA), myeloperoxidase (MPO), advanced oxidation protein products (AOPP), catalase (CAT), paraoxonase (PON1) and platelet-activating factor acetylhydrolase (PAF-AH) analyses. Results: When compared with the control group; the serum IMA levels significantly decreased in group II, and also increased in group III as compared to control (p<0.05). IMA levels were also significantly decreased in both groups II and IV as compared to group III (p<0.001). Serum MPO activity increased in group III as compared to control (p<0.05). Serum AOPP levels were significantly increased in group III as compared to group II (p<0.01) and decreased in group IV as compared to group III (p<0.01). Serum CAT activity was higher in group II than controls (p<0.05). Serum PON and plasma PAF-AH activities significantly decreased in grup III as compared to group II (p<0.05) and plasma PAF-AH activity were decreased in group III as compared to controls (p<0.05). Conclusion: In conclusion, administration of dexmedetomidine; prior to ischemia reperfusion injury caused by pneumoperitoneum; reduces the oxidative injury and increases the antioxidant activity in the acute period.
{"title":"The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum","authors":"N. Taş","doi":"10.5505/TJB.2014.79836","DOIUrl":"https://doi.org/10.5505/TJB.2014.79836","url":null,"abstract":"Objective: The aim of the study was to investigate the changes of oxidative and anti-oxidative systems in the splanchnic area during carbon dioxide pneumoperitoneum and to determine whether the administration of dexmedetomidine has effects on these systems. Methods: Forty rats were randomized into four groups: Group I; Control, Group II; No pneumoperitoneum, Dexmedetomidine administration, Group III; Pneumoperitoneum, no Dexmedetomidine administration and Group IV; Pneumoperitoneum and Dexmedetomidine administration 30 minutes before insufflation. The rats were rested 30 minutes after desufflation and blood samples were obtained for; ischaemia modified albumin (IMA), myeloperoxidase (MPO), advanced oxidation protein products (AOPP), catalase (CAT), paraoxonase (PON1) and platelet-activating factor acetylhydrolase (PAF-AH) analyses. Results: When compared with the control group; the serum IMA levels significantly decreased in group II, and also increased in group III as compared to control (p<0.05). IMA levels were also significantly decreased in both groups II and IV as compared to group III (p<0.001). Serum MPO activity increased in group III as compared to control (p<0.05). Serum AOPP levels were significantly increased in group III as compared to group II (p<0.01) and decreased in group IV as compared to group III (p<0.01). Serum CAT activity was higher in group II than controls (p<0.05). Serum PON and plasma PAF-AH activities significantly decreased in grup III as compared to group II (p<0.05) and plasma PAF-AH activity were decreased in group III as compared to controls (p<0.05). Conclusion: In conclusion, administration of dexmedetomidine; prior to ischemia reperfusion injury caused by pneumoperitoneum; reduces the oxidative injury and increases the antioxidant activity in the acute period.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"49 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87299269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The purpose of this study is to determine the median values of the triple and double test screening parameters in Adiyaman region. Methods: Between January 2010-December 2011, triple tests were performed to 5909, double tests were performed to 5310 singleton pregnant women in Adiyaman KadinDogum Hastanesi Biochemistry Laboratory. Serum alpha-fetoprotein (AFP), unconjugated estriol (uE3), beta-human chorionic gonadotrophin (β-hCG) for triple test and serum β-hCG, pregnancy associated plasma protein-A (PAPP-A) for double test were investigated from the patients archives retrospectively. The new calculated medians of the Adiyaman region and the existing computer program’s medians were evaluated statistically. Results: The first trimester screening parameters’ median values of beta-human chorionic gonadotrophin and pregnancy associated plasma protein-A were significantly lower than the program’s median values (p<0.05). The medians of AFP were significantly lower than the program’s medians in 15-19 gestation weeks (p<0.05). The medians of uE3 were significantly higher than the programmes’ medians (p<0.05) except 20. gestation week. β-hCG medians of 16,19 and 20’th gestational weeks were statistically different than the programme’s medians (p<0.05), the difference between 15, 17 and 18’th gestational weeks’ values were statistically unnecessary. The calculated first trimester screening parameters’ median values of β-hCG and PAPPA were significantly lower than the program’s median values in all gestational weeks (p<0.05). For triple test the medians of AFP were lower than the programme’s medians in all weeks and this was statistically significant except 20th gestational week (p<0.05). The medians of uE3 were significantly higher than the programs’ medians (p<0.05) except 20th gestation week. β-hCG medians of 16,19 and 20th gestational weeks were statistically different than the programm’s medians (p<0.05), the difference between 15., 17. and 18th gestational weeks’ values were not statistically significant. Conclusion: The screening test performances will be affected by using the regional median values as in former studies.
{"title":"Determination of the median levels of double and triple prenatal screening parameters in Adıyaman region","authors":"P. G. Atak, A. Arpacı, Gulsah Seydal","doi":"10.5505/TJB.2014.20591","DOIUrl":"https://doi.org/10.5505/TJB.2014.20591","url":null,"abstract":"Objective: The purpose of this study is to determine the median values of the triple and double test screening parameters in Adiyaman region. Methods: Between January 2010-December 2011, triple tests were performed to 5909, double tests were performed to 5310 singleton pregnant women in Adiyaman KadinDogum Hastanesi Biochemistry Laboratory. Serum alpha-fetoprotein (AFP), unconjugated estriol (uE3), beta-human chorionic gonadotrophin (β-hCG) for triple test and serum β-hCG, pregnancy associated plasma protein-A (PAPP-A) for double test were investigated from the patients archives retrospectively. The new calculated medians of the Adiyaman region and the existing computer program’s medians were evaluated statistically. Results: The first trimester screening parameters’ median values of beta-human chorionic gonadotrophin and pregnancy associated plasma protein-A were significantly lower than the program’s median values (p<0.05). The medians of AFP were significantly lower than the program’s medians in 15-19 gestation weeks (p<0.05). The medians of uE3 were significantly higher than the programmes’ medians (p<0.05) except 20. gestation week. β-hCG medians of 16,19 and 20’th gestational weeks were statistically different than the programme’s medians (p<0.05), the difference between 15, 17 and 18’th gestational weeks’ values were statistically unnecessary. The calculated first trimester screening parameters’ median values of β-hCG and PAPPA were significantly lower than the program’s median values in all gestational weeks (p<0.05). For triple test the medians of AFP were lower than the programme’s medians in all weeks and this was statistically significant except 20th gestational week (p<0.05). The medians of uE3 were significantly higher than the programs’ medians (p<0.05) except 20th gestation week. β-hCG medians of 16,19 and 20th gestational weeks were statistically different than the programm’s medians (p<0.05), the difference between 15., 17. and 18th gestational weeks’ values were not statistically significant. Conclusion: The screening test performances will be affected by using the regional median values as in former studies.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"84 1","pages":"231-237"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90301410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Babu, M. N. Jyothi, U. Shivaram, S. Narayanaswamy, D. Rai, V. R. Devaraj
Objective: In this study, we report the role of miRNAs involved under nitrogen starvation from widely grown vegetable crop, French bean. In recent years, a great deal of attention has been paid to the elucidation of miRNAs involved in low nitrate stress. Methods: To identify miRNAs expressed under stress, cDNA libraries were analyzed. Results: We reported the nine potential miRNAs with 67 targets involved in nutrient transporters and other stress specific genes. Among the miRNA sequences obtained 6 sequences belong to miR172 family, one with miR169. RT-PCR analysis of expression of miR172 family was induced upon low nitrate stress while miR169 family was repressed. In addition, Pvu-SN7b and Pvu-miR16 may be new members of miRNA172 and miR169 families, respectively. Conclusion: The targets of Pvu-SN7b were major protein kinases, one among which is the Protein Kinase CK2. CK2 Kinase is found to involve in transcription-directed signaling, gene control and cell-cycle regulation. Other targets of Pvu-SN7b were involved in DNA-dependent transcription regulation, photo-periodism, calcium-mediated signaling. Pvu-miR16 targets Thymidine kinase, the key enzyme of deoxy-nucleotide synthesis. The cleavage of these targets affects cell proliferation there by affecting nodule formation. Pvu-miR8 inhibits translation of its target protein Pre-protein translocase, a membrane-bound protein transporter involved in trans-membrane protein transportation. Together these results denote the response and role of miRNAs to nitrate-limiting conditions in French bean.
{"title":"Identification of miRNAs from French bean (Phaseolus vulgaris) under low nitrate stress","authors":"N. Babu, M. N. Jyothi, U. Shivaram, S. Narayanaswamy, D. Rai, V. R. Devaraj","doi":"10.5505/TJB.2014.20982","DOIUrl":"https://doi.org/10.5505/TJB.2014.20982","url":null,"abstract":"Objective: In this study, we report the role of miRNAs involved under nitrogen starvation from widely grown vegetable crop, French bean. In recent years, a great deal of attention has been paid to the elucidation of miRNAs involved in low nitrate stress. Methods: To identify miRNAs expressed under stress, cDNA libraries were analyzed. Results: We reported the nine potential miRNAs with 67 targets involved in nutrient transporters and other stress specific genes. Among the miRNA sequences obtained 6 sequences belong to miR172 family, one with miR169. RT-PCR analysis of expression of miR172 family was induced upon low nitrate stress while miR169 family was repressed. In addition, Pvu-SN7b and Pvu-miR16 may be new members of miRNA172 and miR169 families, respectively. Conclusion: The targets of Pvu-SN7b were major protein kinases, one among which is the Protein Kinase CK2. CK2 Kinase is found to involve in transcription-directed signaling, gene control and cell-cycle regulation. Other targets of Pvu-SN7b were involved in DNA-dependent transcription regulation, photo-periodism, calcium-mediated signaling. Pvu-miR16 targets Thymidine kinase, the key enzyme of deoxy-nucleotide synthesis. The cleavage of these targets affects cell proliferation there by affecting nodule formation. Pvu-miR8 inhibits translation of its target protein Pre-protein translocase, a membrane-bound protein transporter involved in trans-membrane protein transportation. Together these results denote the response and role of miRNAs to nitrate-limiting conditions in French bean.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"9 1","pages":"1-18"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78464893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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