Aim: To clone genes encoding analgesic peptides from the cDNA pool of scorpion telson, to obtain recombinant peptides by prokaryotic expression system and examine their analgesic activity in mouse twisting test. Material and methods: The genes encoding analgesic peptides were cloned from the cDNA pool of scorpion telson by nested PCR. Positive clones were sequenced after screened by PCR-SSCP. The recombinant peptides were obtained by functionally expression in E. coli and purified by metal chelating chromatography. The bioactivity was assayed in mouse twisting test. Results: Two nucleotide sequences encoding potential analgesic peptides were obtained. They were named as BmK 22 and BmK 9. BmK 22 was a new peptide with only one amino acid at site 54 different with BmK 9. In mouse-twisting test, both of the two recombinant peptides exhibited analgesic activity, and BmK 9 showed a stronger activity in pain relieving. Further, when considering structure factors by homology modeling, we speculated that the Arg residue at site 54 of BmK 9 may play an important role in target recognition and influence the analgesic activity. Conclusion: Venoms from scorpions contain extremely rich bioactive peptides. The strategy in this paper involving molecular cloning, functional expression and bioactivity identification of BmK 9 and BmK 22 provided a rapid route to discover scorpion toxins with special bioactivity such as analgesics.
{"title":"Molecular cloning and functional identification of analgesic peptides from Buthus martensii Karsch","authors":"Jianhua Shao, Chun-Chao Zhao, Changye Hui, Rui Zhao, Xiaoyang Ruan","doi":"10.5505/TJB.2013.84429","DOIUrl":"https://doi.org/10.5505/TJB.2013.84429","url":null,"abstract":"Aim: To clone genes encoding analgesic peptides from the cDNA pool of scorpion telson, to obtain recombinant peptides by prokaryotic expression system and examine their analgesic activity in mouse twisting test. Material and methods: The genes encoding analgesic peptides were cloned from the cDNA pool of scorpion telson by nested PCR. Positive clones were sequenced after screened by PCR-SSCP. The recombinant peptides were obtained by functionally expression in E. coli and purified by metal chelating chromatography. The bioactivity was assayed in mouse twisting test. Results: Two nucleotide sequences encoding potential analgesic peptides were obtained. They were named as BmK 22 and BmK 9. BmK 22 was a new peptide with only one amino acid at site 54 different with BmK 9. In mouse-twisting test, both of the two recombinant peptides exhibited analgesic activity, and BmK 9 showed a stronger activity in pain relieving. Further, when considering structure factors by homology modeling, we speculated that the Arg residue at site 54 of BmK 9 may play an important role in target recognition and influence the analgesic activity. Conclusion: Venoms from scorpions contain extremely rich bioactive peptides. The strategy in this paper involving molecular cloning, functional expression and bioactivity identification of BmK 9 and BmK 22 provided a rapid route to discover scorpion toxins with special bioactivity such as analgesics.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"34 1","pages":"499-505"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88503042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ata, F. H. Wattoo, Imran Qasim, M. H. S. Wattoo, S. A. Tirmizi, M. Qadir
Aim: Quality of underground and Indus river surface water at Kalabagh, Pakistan was monitored to investigate the anthropogenic activities in the region because people of the Mianwali district often suffer from rusty spot on their teeth and clothes. Material and Methods: Fully flush sampling method was used for underground water samples. Surface water samples were collected from the main river flow. Conductivity, total dissolved solid (TDS), pH, chemical oxygen demand (COD) and biochemical oxygen demand (BOD) were measured using the standard procedures. Heavy metals were determined by plasma atomic emission spectrophotometry. Results: The obtained results were compared with the set limits of National Environmental Quality Standards (NEQs) and World Health Organization (WHO). In river water samples, the average levels for BOD, COD, TDS, conductivity, pH and heavy metals were exceeding the limits of NEQs and WHO. In underground water samples of Kamer village, levels for COD, BOD, TDS and heavy metals such as cadmium and chromium were below their maximum contamination limits (MCL). However, the levels for pH, conductivity, iron and manganese were above the limits of MCL. In underground water samples from Mianwali city, the parameters including BOD, COD, TDS and heavy metals, including cadmium and chromium were below their MCL, while the conductivity, pH, and heavy metals were also observed higher than their MCL. Conclusion: The investigated parameters for river water like dissolved oxygen (DO), BOD, COD, TDS, iron, manganese, lead, cadmium were reported above MCL. In underground drinking water of Kamer village and river water samples of Mianwali city areas, the concentration levels for lead, iron and manganese were also found higher than their MCL. This may be one of cause for rusty spot on teeth and clothes of the residents. The statistical linear correlation study indicates that metals might have their origin from anthropogenic activities and natural influences.
{"title":"Monitoring of anthropogenic influences on underground and surface water quality of Indus River at district Mianwali-Pakistan","authors":"S. Ata, F. H. Wattoo, Imran Qasim, M. H. S. Wattoo, S. A. Tirmizi, M. Qadir","doi":"10.5505/TJB.2013.66588","DOIUrl":"https://doi.org/10.5505/TJB.2013.66588","url":null,"abstract":"Aim: Quality of underground and Indus river surface water at Kalabagh, Pakistan was monitored to investigate the anthropogenic activities in the region because people of the Mianwali district often suffer from rusty spot on their teeth and clothes. Material and Methods: Fully flush sampling method was used for underground water samples. Surface water samples were collected from the main river flow. Conductivity, total dissolved solid (TDS), pH, chemical oxygen demand (COD) and biochemical oxygen demand (BOD) were measured using the standard procedures. Heavy metals were determined by plasma atomic emission spectrophotometry. Results: The obtained results were compared with the set limits of National Environmental Quality Standards (NEQs) and World Health Organization (WHO). In river water samples, the average levels for BOD, COD, TDS, conductivity, pH and heavy metals were exceeding the limits of NEQs and WHO. In underground water samples of Kamer village, levels for COD, BOD, TDS and heavy metals such as cadmium and chromium were below their maximum contamination limits (MCL). However, the levels for pH, conductivity, iron and manganese were above the limits of MCL. In underground water samples from Mianwali city, the parameters including BOD, COD, TDS and heavy metals, including cadmium and chromium were below their MCL, while the conductivity, pH, and heavy metals were also observed higher than their MCL. Conclusion: The investigated parameters for river water like dissolved oxygen (DO), BOD, COD, TDS, iron, manganese, lead, cadmium were reported above MCL. In underground drinking water of Kamer village and river water samples of Mianwali city areas, the concentration levels for lead, iron and manganese were also found higher than their MCL. This may be one of cause for rusty spot on teeth and clothes of the residents. The statistical linear correlation study indicates that metals might have their origin from anthropogenic activities and natural influences.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":" 31","pages":"25-30"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5505/TJB.2013.66588","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72536183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ozlem Karatoprak, Z. Karaali, Kutluhan Incekara, Faruk Çelik, U. Zeybek, A. Ergen
Objective: The ABCB1/MDR1 is a 170-kDa transporter protein called P-glycoprotein (Pgp) which has been associated with the transport of cellular lipids and drugs. Recent studies focused on MDR1, and its effects on lipid transport, show that the constitutive expression of P-gp transporter in normal tissues plays an important role in drug disposition and response. In addition, it is known that some polymorphisms on the MDR1 gene alter the expression of the P-gp. This study aims to investigate the effects of the MDR1 C3435T and the C1236T gene polymorphisms on the dyslipidemia of diabetic patients. Material and methods: The PCR-RFLP method has been used on 77 patients and 75 controls in order to determine the MDR1 genotype. Results: There are no statistical differences for the MDR1 C3435T and the C1236T genotype ranges among between our inspected groups. However, the C1236T mutant type T allele ratio increases statistically in the patient group (p = 0.026 OR: 1.679 % 95 CI: 1.062 – 2.652 ). A weak connection has been observed between the MDR1 C3435T C and the C1236T C alleles, according to the linkage disequilibrium analysis. Conclusion: This study is one of the preceding studies, which examines the relationship between MDR1 polymorphisms and type 2 diabetes. Studies on MDR1 genotypes, and their effects on lipid levels are new in literature, hence our study proves to be unique when it comes to both the C3435T and the C1236T polymorphisms in type 2 diabetes patients; however, further research is still needed for the confirmation of our findings.
{"title":"The role of mdr1 gene polymorphisms in type 2 Diabetes Mellitus","authors":"Ozlem Karatoprak, Z. Karaali, Kutluhan Incekara, Faruk Çelik, U. Zeybek, A. Ergen","doi":"10.5505/TJB.2013.41736","DOIUrl":"https://doi.org/10.5505/TJB.2013.41736","url":null,"abstract":"Objective: The ABCB1/MDR1 is a 170-kDa transporter protein called P-glycoprotein (Pgp) which has been associated with the transport of cellular lipids and drugs. Recent studies focused on MDR1, and its effects on lipid transport, show that the constitutive expression of P-gp transporter in normal tissues plays an important role in drug disposition and response. In addition, it is known that some polymorphisms on the MDR1 gene alter the expression of the P-gp. This study aims to investigate the effects of the MDR1 C3435T and the C1236T gene polymorphisms on the dyslipidemia of diabetic patients. Material and methods: The PCR-RFLP method has been used on 77 patients and 75 controls in order to determine the MDR1 genotype. Results: There are no statistical differences for the MDR1 C3435T and the C1236T genotype ranges among between our inspected groups. However, the C1236T mutant type T allele ratio increases statistically in the patient group (p = 0.026 OR: 1.679 % 95 CI: 1.062 – 2.652 ). A weak connection has been observed between the MDR1 C3435T C and the C1236T C alleles, according to the linkage disequilibrium analysis. Conclusion: This study is one of the preceding studies, which examines the relationship between MDR1 polymorphisms and type 2 diabetes. Studies on MDR1 genotypes, and their effects on lipid levels are new in literature, hence our study proves to be unique when it comes to both the C3435T and the C1236T polymorphisms in type 2 diabetes patients; however, further research is still needed for the confirmation of our findings.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"1 1","pages":"186-192"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77771813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Zare-Zardini, L. Ebrahimi, M. Ejtehadi, A. Hashemi, Adel Ghorani Azam, A. Atefi, Mojgan Soleimanizadeh
Objective: Antimicrobial peptides are the most important agents in innate immune system. These peptides have suitable activities against different pathogens. The aim of this study was to purify new antimicrobial peptides from skin secretion of Bufo kavirensis. Material and Methods: Purification of peptides was carried out by reverse phase-HPLC and its sequence was determined by using MS/MS. The antimicrobial activity was tested by Radial Diffusion Assay (RDA) and Minimal Inhibitory Concentration (MIC) methods. Results: This peptide is composed of 20 amino acids with the sequence of: ILGPVLGLVGRLAGGLIKRE. There is no similarity between this new and novel peptide which is named Maximin Bk and other antimicrobial peptides. The Maximin Bk showed considerable antimicrobial activity against gram positive and gram negative bacteria (Minimum Inhibitory Concentrations (MIC 8.1 to 20.78 µg/ml) as well as fungi (MIC, 25.7 to 35.6 mg/ml). Buforin -K showed virtually low hemolytic activity, 5% hemolysis in a concentration of 100 μg/mL. Conclusion: The new antimicrobial peptide from skin secretion of Bufo kavirensis showed higher antimicrobial activity against gram negative than against gram positive bacteria and fungi. In base of high antimicrobial and low hemolytic activity, Maximin Bk is the potent peptide for treatment of various microbial diseases.
{"title":"Purification and characterization of one novel cationic antimicrobial peptide from skin secretion of Bufo kavirensis","authors":"H. Zare-Zardini, L. Ebrahimi, M. Ejtehadi, A. Hashemi, Adel Ghorani Azam, A. Atefi, Mojgan Soleimanizadeh","doi":"10.5505/TJB.2013.26818","DOIUrl":"https://doi.org/10.5505/TJB.2013.26818","url":null,"abstract":"Objective: Antimicrobial peptides are the most important agents in innate immune system. These peptides have suitable activities against different pathogens. The aim of this study was to purify new antimicrobial peptides from skin secretion of Bufo kavirensis. Material and Methods: Purification of peptides was carried out by reverse phase-HPLC and its sequence was determined by using MS/MS. The antimicrobial activity was tested by Radial Diffusion Assay (RDA) and Minimal Inhibitory Concentration (MIC) methods. Results: This peptide is composed of 20 amino acids with the sequence of: ILGPVLGLVGRLAGGLIKRE. There is no similarity between this new and novel peptide which is named Maximin Bk and other antimicrobial peptides. The Maximin Bk showed considerable antimicrobial activity against gram positive and gram negative bacteria (Minimum Inhibitory Concentrations (MIC 8.1 to 20.78 µg/ml) as well as fungi (MIC, 25.7 to 35.6 mg/ml). Buforin -K showed virtually low hemolytic activity, 5% hemolysis in a concentration of 100 μg/mL. Conclusion: The new antimicrobial peptide from skin secretion of Bufo kavirensis showed higher antimicrobial activity against gram negative than against gram positive bacteria and fungi. In base of high antimicrobial and low hemolytic activity, Maximin Bk is the potent peptide for treatment of various microbial diseases.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"18 1","pages":"416-424"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79962262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Ademoglu, K. Ozcan, Sevda Tanrıkulu-Küçük, F. Gurdol
Objective: The aim of this study was to examine the ageand gender-related differences in peroxide production, manganese superoxide dismutase (MnSOD) activity and expression in liver mitochondria of Wistar rats at 12 and 24 months of age. Methods: The chemiluminometric method for peroxide production, fluorometric method for malondialdehyde (MDA) levels, cumene hydroperoxide assay for glutathione peroxidase (GPx), the nitroblue tetrazolium assay for MnSOD activity, and Western-blotting for MnSOD expression were used. Results: Mitochondrial peroxides are increased significantly in both genders as aging proceeded, and females exhibited more profound increment than the males. Mitochondrial SOD and GPx activities remained unaltered between 12 and 24 months of age, with no difference between two genders. The genderand age-related differences were observed in MnSOD expression (p<0.01). The SOD activity per expressed enzyme protein was significantly decreased in 24-month-old animals of both genders (p<0.01). Female rats had a significantly lower ratio than their male counterparts (p<0.05). In females, the expression was not associated with the activity of MnSOD, while a positive correlation existed between these parameters in males (r=0.573, p=0.001). Enzyme expression was found to be significantly higher in female rats as compared to their male counterparts. Liver mitochondria are less prone to oxidative damage in female rats compared to males as observed at 12 and 24 months of age Conclusions: An involvement of factors other than estrogen seems to be relevant for the difference in the MnSOD activity and expression pattern between two genders.
{"title":"Age-related changes in the activity and expression of manganese superoxide dismutase, and mitochondrial oxidant generation in female and male rats","authors":"E. Ademoglu, K. Ozcan, Sevda Tanrıkulu-Küçük, F. Gurdol","doi":"10.5505/TJB.2013.93898","DOIUrl":"https://doi.org/10.5505/TJB.2013.93898","url":null,"abstract":"Objective: The aim of this study was to examine the ageand gender-related differences in peroxide production, manganese superoxide dismutase (MnSOD) activity and expression in liver mitochondria of Wistar rats at 12 and 24 months of age. Methods: The chemiluminometric method for peroxide production, fluorometric method for malondialdehyde (MDA) levels, cumene hydroperoxide assay for glutathione peroxidase (GPx), the nitroblue tetrazolium assay for MnSOD activity, and Western-blotting for MnSOD expression were used. Results: Mitochondrial peroxides are increased significantly in both genders as aging proceeded, and females exhibited more profound increment than the males. Mitochondrial SOD and GPx activities remained unaltered between 12 and 24 months of age, with no difference between two genders. The genderand age-related differences were observed in MnSOD expression (p<0.01). The SOD activity per expressed enzyme protein was significantly decreased in 24-month-old animals of both genders (p<0.01). Female rats had a significantly lower ratio than their male counterparts (p<0.05). In females, the expression was not associated with the activity of MnSOD, while a positive correlation existed between these parameters in males (r=0.573, p=0.001). Enzyme expression was found to be significantly higher in female rats as compared to their male counterparts. Liver mitochondria are less prone to oxidative damage in female rats compared to males as observed at 12 and 24 months of age Conclusions: An involvement of factors other than estrogen seems to be relevant for the difference in the MnSOD activity and expression pattern between two genders.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"37 1","pages":"445-450"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86878893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rett syndrome is an X-linked dominant neurodevelopmental disorder which is primarily seen in girls. Mutations in the MECP2 gene are responsible for 80% of affected patients. The most common mutations are found in exons 3, 4 of this gene. Most MECP2 alterations are de novo and the recurrence risk is low. Approximately 1% of all affected patients are thought to be familial and clinically unaffected carrier mothers have been reported. Here, we present 3 year old girl patient who had all of the diagnostic criteria for typical Rett syndrome. The de novo, heterozygous c.808C>T mutation was detected by sequence analysis of exon 3 in the MECP2 gene. We report this patient to emphasize the importance of the steps followed in the molecular analysis in Rett syndrome. Hovewer, when the mutation was detected in a patient, the molecular analysis of the mother is extremely important for correct genetic counseling.
{"title":"Genetic Approach to the Patient and the Family: A Rett Syndrome Case with R270X Mutation in MECP2 Gene","authors":"F. Hazan, O. Güzel, M. Çeleğen, A. Tükün","doi":"10.5505/TJB.2013.07742","DOIUrl":"https://doi.org/10.5505/TJB.2013.07742","url":null,"abstract":"Rett syndrome is an X-linked dominant neurodevelopmental disorder which is primarily seen in girls. Mutations in the MECP2 gene are responsible for 80% of affected patients. The most common mutations are found in exons 3, 4 of this gene. Most MECP2 alterations are de novo and the recurrence risk is low. Approximately 1% of all affected patients are thought to be familial and clinically unaffected carrier mothers have been reported. Here, we present 3 year old girl patient who had all of the diagnostic criteria for typical Rett syndrome. The de novo, heterozygous c.808C>T mutation was detected by sequence analysis of exon 3 in the MECP2 gene. We report this patient to emphasize the importance of the steps followed in the molecular analysis in Rett syndrome. Hovewer, when the mutation was detected in a patient, the molecular analysis of the mother is extremely important for correct genetic counseling.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"183 1","pages":"109-113"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74929106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eray Özgün, G. S. Ozgun, S. Eskiocak, O. Yalcin, S. S. Gökmen
Aim: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. We investigated antioxidant L-carnitine effect on activities of paraoxonase 1 enzyme which is also synthesized in colon and oxidative status in experimental colitis. Material and Methods: Wistar albino female rats were divided into four groups randomly: control, colitis, pre-treatment and treatment groups. To induce colitis, single dose of 1 mL acetic acid (%4) was given intrarectally to colitis, pre-treatment and treatment groups. Single dose of 500 mg/kg L-carnitine was given intraperitoneally 1 hour before inducing colitis to pre-treatment group and 24 hours after inducing colitis to treatment group. All groups were sacrificied 48 hours after intrarectally administration. Existence of colitis was confirmed by histopathological changes. Paraoxonase, arylesterase and lactonase activities, total oxidant and antioxidant status, malondialdehyde, and total sialic acid were measured in serum. Oxidative stress index was calculated from the formula. Results: While serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index were significantly elevated, serum paraoxonase, arylesterase and lactonase activities and total antioxidant status were significantly decreased in acetic-acid induced experimental colitis. In acetic-acid induced experimental colitis, L-carnitine caused a significant decrease in serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index but a significant increase in serum arylesterase and lactonase activities of treatment group only. Conclusion: L-Carnitine has an increasing effect on serum arylesterase and lactonase activities and decreasing effect on oxidative stress in acetic acid-induced experimental colitis. Therefore, L-carnitine may be useful for the treatment of inflammatory bowel disease.
{"title":"Effect of L-carnitine on serum paraoxonase, arylesterase and lactonase activities and oxidative status in experimental colitis","authors":"Eray Özgün, G. S. Ozgun, S. Eskiocak, O. Yalcin, S. S. Gökmen","doi":"10.5505/TJB.2013.29292","DOIUrl":"https://doi.org/10.5505/TJB.2013.29292","url":null,"abstract":"Aim: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. We investigated antioxidant L-carnitine effect on activities of paraoxonase 1 enzyme which is also synthesized in colon and oxidative status in experimental colitis. Material and Methods: Wistar albino female rats were divided into four groups randomly: control, colitis, pre-treatment and treatment groups. To induce colitis, single dose of 1 mL acetic acid (%4) was given intrarectally to colitis, pre-treatment and treatment groups. Single dose of 500 mg/kg L-carnitine was given intraperitoneally 1 hour before inducing colitis to pre-treatment group and 24 hours after inducing colitis to treatment group. All groups were sacrificied 48 hours after intrarectally administration. Existence of colitis was confirmed by histopathological changes. Paraoxonase, arylesterase and lactonase activities, total oxidant and antioxidant status, malondialdehyde, and total sialic acid were measured in serum. Oxidative stress index was calculated from the formula. Results: While serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index were significantly elevated, serum paraoxonase, arylesterase and lactonase activities and total antioxidant status were significantly decreased in acetic-acid induced experimental colitis. In acetic-acid induced experimental colitis, L-carnitine caused a significant decrease in serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index but a significant increase in serum arylesterase and lactonase activities of treatment group only. Conclusion: L-Carnitine has an increasing effect on serum arylesterase and lactonase activities and decreasing effect on oxidative stress in acetic acid-induced experimental colitis. Therefore, L-carnitine may be useful for the treatment of inflammatory bowel disease.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"12 1","pages":"0-0"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72752308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Koçer, C. Karakukcu, H. Karaman, A. Tasdemir, M. Ergul
Objective: The aim of this study is to evaluate the diagnostic performance of PSA- based parameters in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) and to determine the relation between serum PSA and histopathological grade in PCa. Methods: This retrospective study includes data of 320 patients with PCa (n: 155) and BPH patients (n: 165). Serum PSA levels and Gleason scores of patients were determi- ned by examining the records of Clinical Biochemistry and Pathology Laboratory. We classified the patients according to total PSA (tPSA) levels to determine diagnostic per- formance of PSA-based parameters at different cut-off levels. Serum tPSA, free PSA (fPSA) and complexed PSA (cPSA) were analyzed with chemiluminometric method. Results: There were significant differences between BPH and PCa patients in tPSA, fPSA, cPSA and f/tPSA values (p<0.05) in whole group (WG). There were significant differences between BPH and PCa patients in cPSA and f/tPSA in group with tPSA<4 ng/mL (LG); in f/tPSA values in group with tPSA 4-10 ng/mL (intermediate group, IG). According to histopathological classification, all of the parameters except f/tPSA were significantly dif- ferent between groups in PCa (p<0.001). Significant positive correlations were found bet- ween Gleason scores and tPSA (r=0.577), fPSA (r=0.491) and cPSA (r=0.562) (p<0.001). Conclusion: We suggest the use of f/tPSA to improve the differentiation of BPH and PCa in IG. The best cut-off points for tPSA, fPSA, cPSA and f/tPSA were 4.0, 2.21, 3.16 ng/mL and 0.17 respectively. Based on the results of ROC analysis, a cut-off value of 0.17 for f/tPSA and 3.16 ng/mL for cPSA may be acceptable.
{"title":"PSA-based parameters and their diagnostic performances in patients with prostate cancer and benign prostatic hyperplasia","authors":"D. Koçer, C. Karakukcu, H. Karaman, A. Tasdemir, M. Ergul","doi":"10.5505/TJB.2013.39974","DOIUrl":"https://doi.org/10.5505/TJB.2013.39974","url":null,"abstract":"Objective: The aim of this study is to evaluate the diagnostic performance of PSA- based parameters in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) and to determine the relation between serum PSA and histopathological grade in PCa. Methods: This retrospective study includes data of 320 patients with PCa (n: 155) and BPH patients (n: 165). Serum PSA levels and Gleason scores of patients were determi- ned by examining the records of Clinical Biochemistry and Pathology Laboratory. We classified the patients according to total PSA (tPSA) levels to determine diagnostic per- formance of PSA-based parameters at different cut-off levels. Serum tPSA, free PSA (fPSA) and complexed PSA (cPSA) were analyzed with chemiluminometric method. Results: There were significant differences between BPH and PCa patients in tPSA, fPSA, cPSA and f/tPSA values (p<0.05) in whole group (WG). There were significant differences between BPH and PCa patients in cPSA and f/tPSA in group with tPSA<4 ng/mL (LG); in f/tPSA values in group with tPSA 4-10 ng/mL (intermediate group, IG). According to histopathological classification, all of the parameters except f/tPSA were significantly dif- ferent between groups in PCa (p<0.001). Significant positive correlations were found bet- ween Gleason scores and tPSA (r=0.577), fPSA (r=0.491) and cPSA (r=0.562) (p<0.001). Conclusion: We suggest the use of f/tPSA to improve the differentiation of BPH and PCa in IG. The best cut-off points for tPSA, fPSA, cPSA and f/tPSA were 4.0, 2.21, 3.16 ng/mL and 0.17 respectively. Based on the results of ROC analysis, a cut-off value of 0.17 for f/tPSA and 3.16 ng/mL for cPSA may be acceptable.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"40 1","pages":"37-42"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82653004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Menteşe, S. Guven, A. Sumer, I. Turan, S. Demir, S. Karahan, A. Alver
Aim: The aim of this study is to investigate anti-carbonic anhydrase antibodies (anti-CA I and CA II antibodies) in the sera of women with polycystic ovary syndrome (PCOS). Methods: In this study serum anti-CA I and II antibody levels of age and BMI matching fifty women with PCOS and fifty women without PCOS on day three of menstrual cycle were assessed with an ELISA method previously developed by Hosoda and modified by Alver et al. Results: The mean serum anti-CA I antibody levels were significantly higher in women with PCOS and anti-CA II antibody levels were not significantly different in women with PCOS compared with control subjects. For serum anti-CA I antibody, the absorbance higher than 0.484 (mean + 3SD of control subjects) was taken as positive, Anti-CA I antibody was detected in 13 of 50 patients with PCOS (26%). Considering serum anti-CA II antibody, the absorbance higher than 0.654 (mean + 3SD of control subjects) was taken as positive. Anti- CA II antibody was detected in 2 of 50 patients with PCOS (4%). All patients with positive anti-CA II antibody also had positive anti-CA I antibody. Autoantibodies specifically reacti- ve to CA I were found to be present at a higher frequency than CA II in the serum of subjects with PCOS in the present study. Conclusion: The results may suggest that autoimmune responses against CA I may be invol- ved in the pathogenesis of PCOS.
{"title":"Serum anti-carbonic anhydrase I and II antibodies and polycystic ovary syndrome","authors":"A. Menteşe, S. Guven, A. Sumer, I. Turan, S. Demir, S. Karahan, A. Alver","doi":"10.5505/TJB.2013.44127","DOIUrl":"https://doi.org/10.5505/TJB.2013.44127","url":null,"abstract":"Aim: The aim of this study is to investigate anti-carbonic anhydrase antibodies (anti-CA I and CA II antibodies) in the sera of women with polycystic ovary syndrome (PCOS). Methods: In this study serum anti-CA I and II antibody levels of age and BMI matching fifty women with PCOS and fifty women without PCOS on day three of menstrual cycle were assessed with an ELISA method previously developed by Hosoda and modified by Alver et al. Results: The mean serum anti-CA I antibody levels were significantly higher in women with PCOS and anti-CA II antibody levels were not significantly different in women with PCOS compared with control subjects. For serum anti-CA I antibody, the absorbance higher than 0.484 (mean + 3SD of control subjects) was taken as positive, Anti-CA I antibody was detected in 13 of 50 patients with PCOS (26%). Considering serum anti-CA II antibody, the absorbance higher than 0.654 (mean + 3SD of control subjects) was taken as positive. Anti- CA II antibody was detected in 2 of 50 patients with PCOS (4%). All patients with positive anti-CA II antibody also had positive anti-CA I antibody. Autoantibodies specifically reacti- ve to CA I were found to be present at a higher frequency than CA II in the serum of subjects with PCOS in the present study. Conclusion: The results may suggest that autoimmune responses against CA I may be invol- ved in the pathogenesis of PCOS.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"38 1","pages":"43-48"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79467635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. More, S. DivyalakshmiS, S. N. Prakash, S. Umashankar, J. V. Vishwakarma
Aim: The purification and characterization of an alkaline keratinase from Cunninghamella echinulata. Materials and Methods: Feather-meal medium was used to isolate and screen the fungi. Acetone precipitation and lectin agarose affinity column were used in the purification. The effect of pH, temperature, metal ions and group modifying chemicals was tested. Results: The purified keratinase is a serine protease with a molecular mass of 33kDa with an optimal pH of 4.5 and 10.0, and an optimum temperature of 30°C and 60oC. A 13.2-fold purification was obtained with affinity methods. Discussion: C.echinulata keratinase was inhibited by PMSF, thus it could be a serine pro- tease. Enzyme was inhibited by group specific reagents like TLCK, IAA, NEM and NAI indicating that serine, cysteine, tyrosine and lysine play an important role in the catalytic activity. There was no effect of metal-chelating agent on enzyme activity indicating that the enzyme is not a metalloenzyme; however, it is a metal-activated enzyme as the activity was enhanced by Mn²+. Inhibitory effects of group specific reagents indicated that the enzyme is a serine protease which does not have any divalent ion requirement. The enzyme isolated also has appreciable activity at two different temperatures and pH values making it a versatile organism for industrial applications.
{"title":"Purification and properties of a novel fungal alkaline keratinase from Cunninghamella echinulata","authors":"S. More, S. DivyalakshmiS, S. N. Prakash, S. Umashankar, J. V. Vishwakarma","doi":"10.5505/TJB.2013.37928","DOIUrl":"https://doi.org/10.5505/TJB.2013.37928","url":null,"abstract":"Aim: The purification and characterization of an alkaline keratinase from Cunninghamella echinulata. Materials and Methods: Feather-meal medium was used to isolate and screen the fungi. Acetone precipitation and lectin agarose affinity column were used in the purification. The effect of pH, temperature, metal ions and group modifying chemicals was tested. Results: The purified keratinase is a serine protease with a molecular mass of 33kDa with an optimal pH of 4.5 and 10.0, and an optimum temperature of 30°C and 60oC. A 13.2-fold purification was obtained with affinity methods. Discussion: C.echinulata keratinase was inhibited by PMSF, thus it could be a serine pro- tease. Enzyme was inhibited by group specific reagents like TLCK, IAA, NEM and NAI indicating that serine, cysteine, tyrosine and lysine play an important role in the catalytic activity. There was no effect of metal-chelating agent on enzyme activity indicating that the enzyme is not a metalloenzyme; however, it is a metal-activated enzyme as the activity was enhanced by Mn²+. Inhibitory effects of group specific reagents indicated that the enzyme is a serine protease which does not have any divalent ion requirement. The enzyme isolated also has appreciable activity at two different temperatures and pH values making it a versatile organism for industrial applications.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"49 1","pages":"68-74"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76648196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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