Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.
{"title":"Association of HLA-B27, MEFV gene mutations, ERAP1, IL12B and IL23R gene polymorphisms with ankylosing spondylitis.","authors":"E. Yılmaz","doi":"10.5505/TJB.2014.44265","DOIUrl":"https://doi.org/10.5505/TJB.2014.44265","url":null,"abstract":"Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73377869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and opti- mize the growth conditions for maximum production of phytase. Material and Methods: The screening of isolates was performed on phytase screening me- dium. The best producer was selected. Phytase activity was determined by measur ing the amount of liberated inorganic phosphate. Optimal culture conditions and fermentation pa- rameters for phytase production were assessed. Results: 236 Bacillus sp. strains isolated. The best phytase producing strain showed higher enzyme yield in the presence of wheat bran and lactose as carbon source, meat extract as organic nitrogen source, CaCl 2 as metal source. 0.3% as phytate concentration was found to be best. In the physical parameters, the best results was obtained at 35°C, pH 7.5, 200 rpm as agitation rate, 2-4% as inoculum size and 48 h as inoculum age. A new medium was obtained by optimizing the incubation conditions of phytase production from Bacillus sp. EBD 9-1. In this medium, enzyme yield was enhanced 62% compared to basal medium. Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive.
{"title":"Screening of phytate hydrolysis Bacillus sp. isolated from soil and optimization of the certain nutritional and physical parameters on the production of phytase","authors":"E. Demirkan, E. Baygin, A. Usta","doi":"10.5505/TJB.2014.26817","DOIUrl":"https://doi.org/10.5505/TJB.2014.26817","url":null,"abstract":"Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and opti- mize the growth conditions for maximum production of phytase. Material and Methods: The screening of isolates was performed on phytase screening me- dium. The best producer was selected. Phytase activity was determined by measur ing the amount of liberated inorganic phosphate. Optimal culture conditions and fermentation pa- rameters for phytase production were assessed. Results: 236 Bacillus sp. strains isolated. The best phytase producing strain showed higher enzyme yield in the presence of wheat bran and lactose as carbon source, meat extract as organic nitrogen source, CaCl 2 as metal source. 0.3% as phytate concentration was found to be best. In the physical parameters, the best results was obtained at 35°C, pH 7.5, 200 rpm as agitation rate, 2-4% as inoculum size and 48 h as inoculum age. A new medium was obtained by optimizing the incubation conditions of phytase production from Bacillus sp. EBD 9-1. In this medium, enzyme yield was enhanced 62% compared to basal medium. Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90050693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Adipose Tissue-Derived Inflammatory and Proangiogenic Cytokines on Proliferative Diabetic Retinopathy","authors":"Demet Yabanoğlu","doi":"10.5505/TJB.2014.38039","DOIUrl":"https://doi.org/10.5505/TJB.2014.38039","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73523777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In recent years, metabolic syndrome, which is a serious health problem, enhancing the effect of oxidative stress and lipid peroxidation, which is a natural antioxidant resveratrol, a polyphenol structure, the use of this area has brought. The present study aimed to evaluate the influence of resveratrol (RSV) treatment on heart, kidney and hepatic tissue malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) levels in high fructose feeding rats which form an experimental metabolic syndrome model and compared to pre-treatment of metabolic syndrome group and the control group values. Methods: Wistar/albino rats (n=15) were used in the present study. Rats were divided equally into 3 groups as control, created group of metabolic syndrome and metabolic syndrome plus resveratrol treatment. At the end of the experimental period, tissue MDA, NO, SOD and CAT levels were studied manual spectrophotometric methods after homogenization of tissues. Results: The result of the study, a significant increase in heart and liver tissue MDA levels, and heart, kidney, liver tissue NO levels were observed in the metabolic syndrome group compared to the controls (p<0.05), following treatment with resveratrol significantly decreases were observed to be close to the control values. Kidney and liver tissue catalase activity statistically significant decrease in metabolic syndrome group compared to the controls, (p=0.019 and p=0.021, respectively), kidney and liver tissue catalase activity similar to control values after treatment with resveratrol, a significant increase was observed (p=0.011 and p=0.58), but changes in the activity of SOD has not been as significant as catalase (p>0.05). However, hepatic SOD activity of resveratrol treatment group compared with the control group, a significant increase was recorded in the treated group (p=0.011). Conclusion: These results suggest probably antioxidant effects of resveratrol to reduce oxidative stress in the liver and kidney tissue which is an important role in metabolism. Against tissue damage generated by exogenous fructose, resveratrol is effective in preventing tissue damage with a direct or indirect effect shows.
{"title":"Effects of resveratrol on Oxidant and Antioxidant Systems in Model of Rat Metabolic Syndrome","authors":"N. Ilhan","doi":"10.5505/TJB.2014.58569","DOIUrl":"https://doi.org/10.5505/TJB.2014.58569","url":null,"abstract":"Objective: In recent years, metabolic syndrome, which is a serious health problem, enhancing the effect of oxidative stress and lipid peroxidation, which is a natural antioxidant resveratrol, a polyphenol structure, the use of this area has brought. The present study aimed to evaluate the influence of resveratrol (RSV) treatment on heart, kidney and hepatic tissue malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) levels in high fructose feeding rats which form an experimental metabolic syndrome model and compared to pre-treatment of metabolic syndrome group and the control group values. Methods: Wistar/albino rats (n=15) were used in the present study. Rats were divided equally into 3 groups as control, created group of metabolic syndrome and metabolic syndrome plus resveratrol treatment. At the end of the experimental period, tissue MDA, NO, SOD and CAT levels were studied manual spectrophotometric methods after homogenization of tissues. Results: The result of the study, a significant increase in heart and liver tissue MDA levels, and heart, kidney, liver tissue NO levels were observed in the metabolic syndrome group compared to the controls (p<0.05), following treatment with resveratrol significantly decreases were observed to be close to the control values. Kidney and liver tissue catalase activity statistically significant decrease in metabolic syndrome group compared to the controls, (p=0.019 and p=0.021, respectively), kidney and liver tissue catalase activity similar to control values after treatment with resveratrol, a significant increase was observed (p=0.011 and p=0.58), but changes in the activity of SOD has not been as significant as catalase (p>0.05). However, hepatic SOD activity of resveratrol treatment group compared with the control group, a significant increase was recorded in the treated group (p=0.011). Conclusion: These results suggest probably antioxidant effects of resveratrol to reduce oxidative stress in the liver and kidney tissue which is an important role in metabolism. Against tissue damage generated by exogenous fructose, resveratrol is effective in preventing tissue damage with a direct or indirect effect shows.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90695460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Fruits and vegetables are the main sources of antioxidant compounds and vitamins. In nature, α-tocopherol, is the most abundant and biologically active form of Vitamin E. The current study was aimed to investigate the antioxidant activity and α-tocopherol content of commercial and freshly squeezed fruit juices. Material and Methods: Apple, apricot and tomatoes fresh and commercial fruit juices were evaluated for their potential antioxidant activities using DPPH and ABTS + radical scavenging capacity assays. The total phenolic and flavonoid content of juices were determined by Folin- Ciocalteu and Aluminium chloride colorimetric methods respectively. The α-tocopherol con- tents of juices were investigated by HPLC-UV chromatographic method. Results: The highest α-tocopherol concentration was found in freshly squeezed tomatoes juice (0.0761±0.20mg/100g) the total phenolic content of freshly squeezed fruit juices mea- sured by Folin-Ciocalteu assay, ranged between 2.10-12.83mg gallic acid equivalents (mg GAE/100ml). The total flavonoid content of samples was expressed as quercetin equiva- lents (mg QE/100ml). The commercial tomatoes juice showed higher content of flavonoid (4.10±0.0126 mg QE/100ml), followed by freshly squeezed apple and apricot fruit juices. The fresh fruit juices of tomatoes showed the lowest content of flavonoid and highest content of total phenolic compounds. Freshly squeezed apple juice and apricot juice were found to exert higher antioxidant activities in ABTS+ and DPPH assays respectively. Conclusion: It was observed that α-tocopherol content and antioxidant capacities and total phenolic and flavonoid concentrations of freshly squeezed juices were higher than commer- cial ones.
{"title":"A Comparative Assessment of α-Tocopherol Content and Antioxidant Activity of Some Fresh and Commercial Fruit Juices","authors":"T. Gönenç, H. Kayalar, T. Erdoğan, B. Kıvçak","doi":"10.5505/TJB.2014.36744","DOIUrl":"https://doi.org/10.5505/TJB.2014.36744","url":null,"abstract":"Aim: Fruits and vegetables are the main sources of antioxidant compounds and vitamins. In nature, α-tocopherol, is the most abundant and biologically active form of Vitamin E. The current study was aimed to investigate the antioxidant activity and α-tocopherol content of commercial and freshly squeezed fruit juices. Material and Methods: Apple, apricot and tomatoes fresh and commercial fruit juices were evaluated for their potential antioxidant activities using DPPH and ABTS + radical scavenging capacity assays. The total phenolic and flavonoid content of juices were determined by Folin- Ciocalteu and Aluminium chloride colorimetric methods respectively. The α-tocopherol con- tents of juices were investigated by HPLC-UV chromatographic method. Results: The highest α-tocopherol concentration was found in freshly squeezed tomatoes juice (0.0761±0.20mg/100g) the total phenolic content of freshly squeezed fruit juices mea- sured by Folin-Ciocalteu assay, ranged between 2.10-12.83mg gallic acid equivalents (mg GAE/100ml). The total flavonoid content of samples was expressed as quercetin equiva- lents (mg QE/100ml). The commercial tomatoes juice showed higher content of flavonoid (4.10±0.0126 mg QE/100ml), followed by freshly squeezed apple and apricot fruit juices. The fresh fruit juices of tomatoes showed the lowest content of flavonoid and highest content of total phenolic compounds. Freshly squeezed apple juice and apricot juice were found to exert higher antioxidant activities in ABTS+ and DPPH assays respectively. Conclusion: It was observed that α-tocopherol content and antioxidant capacities and total phenolic and flavonoid concentrations of freshly squeezed juices were higher than commer- cial ones.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84342453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Ulutas, F. Şahpaz, I. Sarici, Mahmut Uluganyan, Beril Akçimen, M. Celi̇k, Y. Can, İ. Kuru
{"title":"Premarital Hemoglobinopathy Screening Study: Is Kadirli Prevalent Thalassemia Area Of Turkey?","authors":"K. Ulutas, F. Şahpaz, I. Sarici, Mahmut Uluganyan, Beril Akçimen, M. Celi̇k, Y. Can, İ. Kuru","doi":"10.5505/TJB.2014.90217","DOIUrl":"https://doi.org/10.5505/TJB.2014.90217","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84766150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Şeker, A. Oğuz, S. Ozdemir, S. Demirtaş, M. Aylı, K. Mergen
Objective: Although the mechanism is still not so clear, ischemia modified albumin is produced as a result of the occurrence of changes in metal binding regions on the albumin with the production of reactive oxygen species. But it is not known whether this change is the cause or the result of different states. This study is designed to investigate the correlation between hypohemoglobinemia, hypoxemia due to blood loss and IMA. Methods: The research is carried out with 83 patients (28 Male, 55 Female; mean age: 51±22 year) and 19 subject as control group (7Male, 12 Female; mean age 35±18 year). Patients were divided into three sub-groups according to their hemoglobin levels (<8, 8-10, 10-12 g/dL). Additionally, same patients were divided into second sub-group according to their follow-up status as outpatient (40 male, 15 female, mean age: 48 ± 20 year), postoperative (8 male, 11 female, mean age: 49 ± 24 year) and intensive care unit (6 male, 5 female; mean age: 64±20 year) patients. To determine the IMA levels the measurement method based on albumin cobalt binding assay was used. Results: In IMA levels, no significant difference is found between the mild (Hb: 10-12 g/dL) [(n=47; 0,61±0,23 ABSU)], moderate (Hb: 8-10 g/dL) [(n=26; 0.58±0.24 ABSU)] and severe (Hb: <8 g/dL) [(n=9; 0.62±0.12 ABSU)] anemia groups. However, because of acute blood loss related acute hypoxemia; IMA levels of the postoperative patients are statistically higher as compared to control group. As compared to control groups, no statistically significant difference is observed in the levels of the serum IMA in the outpatient group who had minimally invasive surgery and in the intensive care patient group. Conclusion: Our results suggest that; if mycardial oxygenization is questioned in cases of acute and heavy blood loss, ischemia modified albumin is not a valuable test for the indicator of the myocardial ischemia.
{"title":"The evaluation of IMA as a cardiac ischemia marker in the cases of hypohemoglobinemia and hypoxemia due to blood loss","authors":"R. Şeker, A. Oğuz, S. Ozdemir, S. Demirtaş, M. Aylı, K. Mergen","doi":"10.5505/TJB.2014.04274","DOIUrl":"https://doi.org/10.5505/TJB.2014.04274","url":null,"abstract":"Objective: Although the mechanism is still not so clear, ischemia modified albumin is produced as a result of the occurrence of changes in metal binding regions on the albumin with the production of reactive oxygen species. But it is not known whether this change is the cause or the result of different states. This study is designed to investigate the correlation between hypohemoglobinemia, hypoxemia due to blood loss and IMA. Methods: The research is carried out with 83 patients (28 Male, 55 Female; mean age: 51±22 year) and 19 subject as control group (7Male, 12 Female; mean age 35±18 year). Patients were divided into three sub-groups according to their hemoglobin levels (<8, 8-10, 10-12 g/dL). Additionally, same patients were divided into second sub-group according to their follow-up status as outpatient (40 male, 15 female, mean age: 48 ± 20 year), postoperative (8 male, 11 female, mean age: 49 ± 24 year) and intensive care unit (6 male, 5 female; mean age: 64±20 year) patients. To determine the IMA levels the measurement method based on albumin cobalt binding assay was used. Results: In IMA levels, no significant difference is found between the mild (Hb: 10-12 g/dL) [(n=47; 0,61±0,23 ABSU)], moderate (Hb: 8-10 g/dL) [(n=26; 0.58±0.24 ABSU)] and severe (Hb: <8 g/dL) [(n=9; 0.62±0.12 ABSU)] anemia groups. However, because of acute blood loss related acute hypoxemia; IMA levels of the postoperative patients are statistically higher as compared to control group. As compared to control groups, no statistically significant difference is observed in the levels of the serum IMA in the outpatient group who had minimally invasive surgery and in the intensive care patient group. Conclusion: Our results suggest that; if mycardial oxygenization is questioned in cases of acute and heavy blood loss, ischemia modified albumin is not a valuable test for the indicator of the myocardial ischemia.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85272667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Firdose, R. Swaroopa, V. Vinayak, B. D. Ghansham
Objective: The aim of the study was to examine the efficiency of different extraction methods for the determination of total phenolics, flavonoids and alkaloid contents contributing to antioxidant capacity of Delphinium malabaricum. Methods: The extracts of different plant parts (roots, stems and leaves) of Delphinium malabaricum were prepared in aqueous and various organic solvents and the extracts were evaluated for phenolics, flavonoids and alkaloid contents as the equivalents of gallic acid, rutin, and colchicine; respectively. The antioxidant capacity of the extracts was also assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity and ferric reducing antioxidant power assays in both fresh and dry plant tissues and the difference in fresh and dry extracts on phytochemical constituents and antioxidant activities were compared. Results: The aqueous extracts of roots exhibited the highest total phenolic (4.94 mg gallic acid/g fresh weight, 13.4 mg gallic acid/g dry weight) and total alkaloid content (8.05 mg colchicine/g fresh weight, 20.4 mg colchicine/g dry weight) as compared to stem, leaves and other solvent extracts. Whereas, flavonoid contents were found to be highest in the leaf extracts (5.36 mg rutin/g fresh weight, 7.88 mg rutin/g dry weight). Interestingly the aqueous extracts of all the plant parts exhibited highest yield of phenolic, flavonoids and alkaloids as compare to the other solvents used for the extraction. Antioxidant activity assays exhibited considerable antioxidant potential and showed expected significant positive correlation with the phytochemical compounds. Conclusion: The study specified that aqueous extracts are more effective to extract phenols, flavonoids, alkaloids and antioxidants from Delphinium malabaricum than organic extracts and roots have higher level and the alkaloids were found to be higher comparing to that of phenolics and flavonoids content per gram dry weight of plant tissue.
{"title":"An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz","authors":"R. Firdose, R. Swaroopa, V. Vinayak, B. D. Ghansham","doi":"10.5505/TJB.2014.47965","DOIUrl":"https://doi.org/10.5505/TJB.2014.47965","url":null,"abstract":"Objective: The aim of the study was to examine the efficiency of different extraction methods for the determination of total phenolics, flavonoids and alkaloid contents contributing to antioxidant capacity of Delphinium malabaricum. Methods: The extracts of different plant parts (roots, stems and leaves) of Delphinium malabaricum were prepared in aqueous and various organic solvents and the extracts were evaluated for phenolics, flavonoids and alkaloid contents as the equivalents of gallic acid, rutin, and colchicine; respectively. The antioxidant capacity of the extracts was also assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity and ferric reducing antioxidant power assays in both fresh and dry plant tissues and the difference in fresh and dry extracts on phytochemical constituents and antioxidant activities were compared. Results: The aqueous extracts of roots exhibited the highest total phenolic (4.94 mg gallic acid/g fresh weight, 13.4 mg gallic acid/g dry weight) and total alkaloid content (8.05 mg colchicine/g fresh weight, 20.4 mg colchicine/g dry weight) as compared to stem, leaves and other solvent extracts. Whereas, flavonoid contents were found to be highest in the leaf extracts (5.36 mg rutin/g fresh weight, 7.88 mg rutin/g dry weight). Interestingly the aqueous extracts of all the plant parts exhibited highest yield of phenolic, flavonoids and alkaloids as compare to the other solvents used for the extraction. Antioxidant activity assays exhibited considerable antioxidant potential and showed expected significant positive correlation with the phytochemical compounds. Conclusion: The study specified that aqueous extracts are more effective to extract phenols, flavonoids, alkaloids and antioxidants from Delphinium malabaricum than organic extracts and roots have higher level and the alkaloids were found to be higher comparing to that of phenolics and flavonoids content per gram dry weight of plant tissue.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72559547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gunaydin, S. Turkmen, A. Şahin, A. Sumer, A. Menteşe, S. Turedi, A. Gunduz, S. Karahan
Aim: Stroke is the third most important cause of death after coronary artery disease and cancer, and the most important among those diseases leading to disability. Recent studies have shown that early diagnosis and treatment of patients presenting to the emergency department with stroke can reduce the effect of the disease on mortality and morbidity. The purpose of this study was to determine the diagnostic value of plasma SCUBE1, a novel biochemical marker thought to be capable of use in ischemic conditions, values in the diagnosis of acute ischemic stroke in the emergency department. Materials and Methods: Thirty patients diagnosed with acute ischemic stroke at the Karadeniz Technical University Faculty of Medicine Emergency Department, Turkey, between May and October, 2011, and a control group of 30 healthy volunteers were included. An enzyme-linked immunosorbent assay kit was used to determine SCUBE-1 levels. Patient and control group plasma SCUBE1 values were compared. Results: Mean age in the patient group was 74.50 ± 10.50, and 59.93 ± 12.63 in the control group. Mean 6th hour SCUBE1 value in the patient group was 25.104 ± 15.837 ng/ml, and the mean 12th hour SCUBE1 value was 27.395 ± 14.146 ng/ml. Mean control group SCUBE1 value was 35.019 ± 22.310 ng/ml. Control group SCUBE1 values were higher than those of the patient group. Sixth hour SCUBE value was statistically significant when the patient and control groups were compared with age-adjusted values (p = 0.626). No statistically significant difference was determined between 6th and 12th hour SCUBE1 values (p = 0.334). Conclusion Plasma SCUBE1 values in acute ischemic stroke patients did not rise at significant levels compared to the control group, and are therefore not useful in the early diagnosis of acute ischemic stroke.
{"title":"The diagnostic value of SCUBE1 levels in acute ischemic stroke","authors":"M. Gunaydin, S. Turkmen, A. Şahin, A. Sumer, A. Menteşe, S. Turedi, A. Gunduz, S. Karahan","doi":"10.5505/TJB.2014.43534","DOIUrl":"https://doi.org/10.5505/TJB.2014.43534","url":null,"abstract":"Aim: Stroke is the third most important cause of death after coronary artery disease and cancer, and the most important among those diseases leading to disability. Recent studies have shown that early diagnosis and treatment of patients presenting to the emergency department with stroke can reduce the effect of the disease on mortality and morbidity. The purpose of this study was to determine the diagnostic value of plasma SCUBE1, a novel biochemical marker thought to be capable of use in ischemic conditions, values in the diagnosis of acute ischemic stroke in the emergency department. Materials and Methods: Thirty patients diagnosed with acute ischemic stroke at the Karadeniz Technical University Faculty of Medicine Emergency Department, Turkey, between May and October, 2011, and a control group of 30 healthy volunteers were included. An enzyme-linked immunosorbent assay kit was used to determine SCUBE-1 levels. Patient and control group plasma SCUBE1 values were compared. Results: Mean age in the patient group was 74.50 ± 10.50, and 59.93 ± 12.63 in the control group. Mean 6th hour SCUBE1 value in the patient group was 25.104 ± 15.837 ng/ml, and the mean 12th hour SCUBE1 value was 27.395 ± 14.146 ng/ml. Mean control group SCUBE1 value was 35.019 ± 22.310 ng/ml. Control group SCUBE1 values were higher than those of the patient group. Sixth hour SCUBE value was statistically significant when the patient and control groups were compared with age-adjusted values (p = 0.626). No statistically significant difference was determined between 6th and 12th hour SCUBE1 values (p = 0.334). Conclusion Plasma SCUBE1 values in acute ischemic stroke patients did not rise at significant levels compared to the control group, and are therefore not useful in the early diagnosis of acute ischemic stroke.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78968539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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