Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts. Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 μmol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms. Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.
{"title":"Antioxidant and antimicrobial activity of East Black Sea Region honeys)","authors":"Ö. Ertürk, H. Sahin, S. Kolaylı, M. C. Ayvaz","doi":"10.5505/TJB.2014.77487","DOIUrl":"https://doi.org/10.5505/TJB.2014.77487","url":null,"abstract":"Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts. Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 μmol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms. Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"397 1","pages":"99-106"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85020345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.
{"title":"Association of HLA-B27, MEFV gene mutations, ERAP1, IL12B and IL23R gene polymorphisms with ankylosing spondylitis.","authors":"E. Yılmaz","doi":"10.5505/TJB.2014.44265","DOIUrl":"https://doi.org/10.5505/TJB.2014.44265","url":null,"abstract":"Objective: Genetic factors have an important role in the pathogenesis of ankylosing spondylitis (AS). The aim of this study was to analyse the association of HLA-B27, MEFV mutations, IL12B, IL23R and ERAP1 polymorphisms in Turkish patients with ankylosing spondylitis. Methods: One hundred AS patients and 100 healthy controls were examined for HLA-B27, 12 common MEFV mutations, IL12B (rs3213120), IL23R (rs11209026), and ERAP1 (rs30187) polymorphisms (SNPs) by allele specific PCR, revers hybridization and sequencing techniques. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) scores were calculated. Results: Our results confirmed that HLA-B27 was strongly associated with AS (69% vs 7% in controls) (p<0.001, OR: 29.6, 95% CI: 12.3-71.1). We also found an association between uveitis and HLA-B27 positivity in AS patients (p=0.004). The MEFV mutations were significantly frequent in AS patients (40%) compared with healthy controls (22%) (p=0.006, OR: 2.56, 95% CI: 1.3-4.4). We found that ERAP1 rs30187 was significantly associated with AS patients (p=0.033). The rs30187 CT genotype was associated with increased AS risk compared to CC or TT genotypes (OR: 2.1, 95% CI: 1.2-3.7). However, in patients with AS carrying the C allele increased the risk 1.9 times (95% Cl: 1.1-3.3). There was no association with AS and IL12B (rs3213120) and IL23R (rs11209026). There were no significant differences between HLA-B27, MEFV mutations, ERAP1 (rs30187) and Bath AS Disease Activity Index (BASDAI), Bath AS Functional Index (BASFI) scores. Conclusion: This study showed that HLA-B27, MEFV mutations and ERAP1 (rs30187) are AS genetic susceptibility genes. Interactions between ERAP1 and HLA-B27 and MEFV mutations may play an important role in the AS pathogenesis.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"50 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73377869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Adipose Tissue-Derived Inflammatory and Proangiogenic Cytokines on Proliferative Diabetic Retinopathy","authors":"Demet Yabanoğlu","doi":"10.5505/TJB.2014.38039","DOIUrl":"https://doi.org/10.5505/TJB.2014.38039","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"41 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73523777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In recent years, metabolic syndrome, which is a serious health problem, enhancing the effect of oxidative stress and lipid peroxidation, which is a natural antioxidant resveratrol, a polyphenol structure, the use of this area has brought. The present study aimed to evaluate the influence of resveratrol (RSV) treatment on heart, kidney and hepatic tissue malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) levels in high fructose feeding rats which form an experimental metabolic syndrome model and compared to pre-treatment of metabolic syndrome group and the control group values. Methods: Wistar/albino rats (n=15) were used in the present study. Rats were divided equally into 3 groups as control, created group of metabolic syndrome and metabolic syndrome plus resveratrol treatment. At the end of the experimental period, tissue MDA, NO, SOD and CAT levels were studied manual spectrophotometric methods after homogenization of tissues. Results: The result of the study, a significant increase in heart and liver tissue MDA levels, and heart, kidney, liver tissue NO levels were observed in the metabolic syndrome group compared to the controls (p<0.05), following treatment with resveratrol significantly decreases were observed to be close to the control values. Kidney and liver tissue catalase activity statistically significant decrease in metabolic syndrome group compared to the controls, (p=0.019 and p=0.021, respectively), kidney and liver tissue catalase activity similar to control values after treatment with resveratrol, a significant increase was observed (p=0.011 and p=0.58), but changes in the activity of SOD has not been as significant as catalase (p>0.05). However, hepatic SOD activity of resveratrol treatment group compared with the control group, a significant increase was recorded in the treated group (p=0.011). Conclusion: These results suggest probably antioxidant effects of resveratrol to reduce oxidative stress in the liver and kidney tissue which is an important role in metabolism. Against tissue damage generated by exogenous fructose, resveratrol is effective in preventing tissue damage with a direct or indirect effect shows.
{"title":"Effects of resveratrol on Oxidant and Antioxidant Systems in Model of Rat Metabolic Syndrome","authors":"N. Ilhan","doi":"10.5505/TJB.2014.58569","DOIUrl":"https://doi.org/10.5505/TJB.2014.58569","url":null,"abstract":"Objective: In recent years, metabolic syndrome, which is a serious health problem, enhancing the effect of oxidative stress and lipid peroxidation, which is a natural antioxidant resveratrol, a polyphenol structure, the use of this area has brought. The present study aimed to evaluate the influence of resveratrol (RSV) treatment on heart, kidney and hepatic tissue malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) levels in high fructose feeding rats which form an experimental metabolic syndrome model and compared to pre-treatment of metabolic syndrome group and the control group values. Methods: Wistar/albino rats (n=15) were used in the present study. Rats were divided equally into 3 groups as control, created group of metabolic syndrome and metabolic syndrome plus resveratrol treatment. At the end of the experimental period, tissue MDA, NO, SOD and CAT levels were studied manual spectrophotometric methods after homogenization of tissues. Results: The result of the study, a significant increase in heart and liver tissue MDA levels, and heart, kidney, liver tissue NO levels were observed in the metabolic syndrome group compared to the controls (p<0.05), following treatment with resveratrol significantly decreases were observed to be close to the control values. Kidney and liver tissue catalase activity statistically significant decrease in metabolic syndrome group compared to the controls, (p=0.019 and p=0.021, respectively), kidney and liver tissue catalase activity similar to control values after treatment with resveratrol, a significant increase was observed (p=0.011 and p=0.58), but changes in the activity of SOD has not been as significant as catalase (p>0.05). However, hepatic SOD activity of resveratrol treatment group compared with the control group, a significant increase was recorded in the treated group (p=0.011). Conclusion: These results suggest probably antioxidant effects of resveratrol to reduce oxidative stress in the liver and kidney tissue which is an important role in metabolism. Against tissue damage generated by exogenous fructose, resveratrol is effective in preventing tissue damage with a direct or indirect effect shows.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"62 3 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90695460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Ulutas, F. Şahpaz, I. Sarici, Mahmut Uluganyan, Beril Akçimen, M. Celi̇k, Y. Can, İ. Kuru
{"title":"Premarital Hemoglobinopathy Screening Study: Is Kadirli Prevalent Thalassemia Area Of Turkey?","authors":"K. Ulutas, F. Şahpaz, I. Sarici, Mahmut Uluganyan, Beril Akçimen, M. Celi̇k, Y. Can, İ. Kuru","doi":"10.5505/TJB.2014.90217","DOIUrl":"https://doi.org/10.5505/TJB.2014.90217","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"33 1","pages":"357-361"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84766150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Şeker, A. Oğuz, S. Ozdemir, S. Demirtaş, M. Aylı, K. Mergen
Objective: Although the mechanism is still not so clear, ischemia modified albumin is produced as a result of the occurrence of changes in metal binding regions on the albumin with the production of reactive oxygen species. But it is not known whether this change is the cause or the result of different states. This study is designed to investigate the correlation between hypohemoglobinemia, hypoxemia due to blood loss and IMA. Methods: The research is carried out with 83 patients (28 Male, 55 Female; mean age: 51±22 year) and 19 subject as control group (7Male, 12 Female; mean age 35±18 year). Patients were divided into three sub-groups according to their hemoglobin levels (<8, 8-10, 10-12 g/dL). Additionally, same patients were divided into second sub-group according to their follow-up status as outpatient (40 male, 15 female, mean age: 48 ± 20 year), postoperative (8 male, 11 female, mean age: 49 ± 24 year) and intensive care unit (6 male, 5 female; mean age: 64±20 year) patients. To determine the IMA levels the measurement method based on albumin cobalt binding assay was used. Results: In IMA levels, no significant difference is found between the mild (Hb: 10-12 g/dL) [(n=47; 0,61±0,23 ABSU)], moderate (Hb: 8-10 g/dL) [(n=26; 0.58±0.24 ABSU)] and severe (Hb: <8 g/dL) [(n=9; 0.62±0.12 ABSU)] anemia groups. However, because of acute blood loss related acute hypoxemia; IMA levels of the postoperative patients are statistically higher as compared to control group. As compared to control groups, no statistically significant difference is observed in the levels of the serum IMA in the outpatient group who had minimally invasive surgery and in the intensive care patient group. Conclusion: Our results suggest that; if mycardial oxygenization is questioned in cases of acute and heavy blood loss, ischemia modified albumin is not a valuable test for the indicator of the myocardial ischemia.
{"title":"The evaluation of IMA as a cardiac ischemia marker in the cases of hypohemoglobinemia and hypoxemia due to blood loss","authors":"R. Şeker, A. Oğuz, S. Ozdemir, S. Demirtaş, M. Aylı, K. Mergen","doi":"10.5505/TJB.2014.04274","DOIUrl":"https://doi.org/10.5505/TJB.2014.04274","url":null,"abstract":"Objective: Although the mechanism is still not so clear, ischemia modified albumin is produced as a result of the occurrence of changes in metal binding regions on the albumin with the production of reactive oxygen species. But it is not known whether this change is the cause or the result of different states. This study is designed to investigate the correlation between hypohemoglobinemia, hypoxemia due to blood loss and IMA. Methods: The research is carried out with 83 patients (28 Male, 55 Female; mean age: 51±22 year) and 19 subject as control group (7Male, 12 Female; mean age 35±18 year). Patients were divided into three sub-groups according to their hemoglobin levels (<8, 8-10, 10-12 g/dL). Additionally, same patients were divided into second sub-group according to their follow-up status as outpatient (40 male, 15 female, mean age: 48 ± 20 year), postoperative (8 male, 11 female, mean age: 49 ± 24 year) and intensive care unit (6 male, 5 female; mean age: 64±20 year) patients. To determine the IMA levels the measurement method based on albumin cobalt binding assay was used. Results: In IMA levels, no significant difference is found between the mild (Hb: 10-12 g/dL) [(n=47; 0,61±0,23 ABSU)], moderate (Hb: 8-10 g/dL) [(n=26; 0.58±0.24 ABSU)] and severe (Hb: <8 g/dL) [(n=9; 0.62±0.12 ABSU)] anemia groups. However, because of acute blood loss related acute hypoxemia; IMA levels of the postoperative patients are statistically higher as compared to control group. As compared to control groups, no statistically significant difference is observed in the levels of the serum IMA in the outpatient group who had minimally invasive surgery and in the intensive care patient group. Conclusion: Our results suggest that; if mycardial oxygenization is questioned in cases of acute and heavy blood loss, ischemia modified albumin is not a valuable test for the indicator of the myocardial ischemia.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"60 1","pages":"221-225"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85272667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Fruits and vegetables are the main sources of antioxidant compounds and vitamins. In nature, α-tocopherol, is the most abundant and biologically active form of Vitamin E. The current study was aimed to investigate the antioxidant activity and α-tocopherol content of commercial and freshly squeezed fruit juices. Material and Methods: Apple, apricot and tomatoes fresh and commercial fruit juices were evaluated for their potential antioxidant activities using DPPH and ABTS + radical scavenging capacity assays. The total phenolic and flavonoid content of juices were determined by Folin- Ciocalteu and Aluminium chloride colorimetric methods respectively. The α-tocopherol con- tents of juices were investigated by HPLC-UV chromatographic method. Results: The highest α-tocopherol concentration was found in freshly squeezed tomatoes juice (0.0761±0.20mg/100g) the total phenolic content of freshly squeezed fruit juices mea- sured by Folin-Ciocalteu assay, ranged between 2.10-12.83mg gallic acid equivalents (mg GAE/100ml). The total flavonoid content of samples was expressed as quercetin equiva- lents (mg QE/100ml). The commercial tomatoes juice showed higher content of flavonoid (4.10±0.0126 mg QE/100ml), followed by freshly squeezed apple and apricot fruit juices. The fresh fruit juices of tomatoes showed the lowest content of flavonoid and highest content of total phenolic compounds. Freshly squeezed apple juice and apricot juice were found to exert higher antioxidant activities in ABTS+ and DPPH assays respectively. Conclusion: It was observed that α-tocopherol content and antioxidant capacities and total phenolic and flavonoid concentrations of freshly squeezed juices were higher than commer- cial ones.
{"title":"A Comparative Assessment of α-Tocopherol Content and Antioxidant Activity of Some Fresh and Commercial Fruit Juices","authors":"T. Gönenç, H. Kayalar, T. Erdoğan, B. Kıvçak","doi":"10.5505/TJB.2014.36744","DOIUrl":"https://doi.org/10.5505/TJB.2014.36744","url":null,"abstract":"Aim: Fruits and vegetables are the main sources of antioxidant compounds and vitamins. In nature, α-tocopherol, is the most abundant and biologically active form of Vitamin E. The current study was aimed to investigate the antioxidant activity and α-tocopherol content of commercial and freshly squeezed fruit juices. Material and Methods: Apple, apricot and tomatoes fresh and commercial fruit juices were evaluated for their potential antioxidant activities using DPPH and ABTS + radical scavenging capacity assays. The total phenolic and flavonoid content of juices were determined by Folin- Ciocalteu and Aluminium chloride colorimetric methods respectively. The α-tocopherol con- tents of juices were investigated by HPLC-UV chromatographic method. Results: The highest α-tocopherol concentration was found in freshly squeezed tomatoes juice (0.0761±0.20mg/100g) the total phenolic content of freshly squeezed fruit juices mea- sured by Folin-Ciocalteu assay, ranged between 2.10-12.83mg gallic acid equivalents (mg GAE/100ml). The total flavonoid content of samples was expressed as quercetin equiva- lents (mg QE/100ml). The commercial tomatoes juice showed higher content of flavonoid (4.10±0.0126 mg QE/100ml), followed by freshly squeezed apple and apricot fruit juices. The fresh fruit juices of tomatoes showed the lowest content of flavonoid and highest content of total phenolic compounds. Freshly squeezed apple juice and apricot juice were found to exert higher antioxidant activities in ABTS+ and DPPH assays respectively. Conclusion: It was observed that α-tocopherol content and antioxidant capacities and total phenolic and flavonoid concentrations of freshly squeezed juices were higher than commer- cial ones.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"10 1","pages":"215-220"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84342453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Ozturk, H. Vatansev, Alpaslan Taner, S. Yılmaz, M. Dağlı, A. Unlu
Objective: Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging.
{"title":"Asymmetric dimethylarginine levels in Behçet's Disease","authors":"B. Ozturk, H. Vatansev, Alpaslan Taner, S. Yılmaz, M. Dağlı, A. Unlu","doi":"10.5505/TJB.2014.74436","DOIUrl":"https://doi.org/10.5505/TJB.2014.74436","url":null,"abstract":"Objective: Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"40 1","pages":"362-367"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76681079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determınatıon Of The Medıan Values Of Trıple Test Screening Parametres İn Yozgat Regıon","authors":"Gokhan Dogukan Akarsu","doi":"10.5505/TJB.2014.25338","DOIUrl":"https://doi.org/10.5505/TJB.2014.25338","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"20 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85936231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：