N. Babu, M. N. Jyothi, U. Shivaram, S. Narayanaswamy, D. Rai, V. R. Devaraj
Objective: In this study, we report the role of miRNAs involved under nitrogen starvation from widely grown vegetable crop, French bean. In recent years, a great deal of attention has been paid to the elucidation of miRNAs involved in low nitrate stress. Methods: To identify miRNAs expressed under stress, cDNA libraries were analyzed. Results: We reported the nine potential miRNAs with 67 targets involved in nutrient transporters and other stress specific genes. Among the miRNA sequences obtained 6 sequences belong to miR172 family, one with miR169. RT-PCR analysis of expression of miR172 family was induced upon low nitrate stress while miR169 family was repressed. In addition, Pvu-SN7b and Pvu-miR16 may be new members of miRNA172 and miR169 families, respectively. Conclusion: The targets of Pvu-SN7b were major protein kinases, one among which is the Protein Kinase CK2. CK2 Kinase is found to involve in transcription-directed signaling, gene control and cell-cycle regulation. Other targets of Pvu-SN7b were involved in DNA-dependent transcription regulation, photo-periodism, calcium-mediated signaling. Pvu-miR16 targets Thymidine kinase, the key enzyme of deoxy-nucleotide synthesis. The cleavage of these targets affects cell proliferation there by affecting nodule formation. Pvu-miR8 inhibits translation of its target protein Pre-protein translocase, a membrane-bound protein transporter involved in trans-membrane protein transportation. Together these results denote the response and role of miRNAs to nitrate-limiting conditions in French bean.
{"title":"Identification of miRNAs from French bean (Phaseolus vulgaris) under low nitrate stress","authors":"N. Babu, M. N. Jyothi, U. Shivaram, S. Narayanaswamy, D. Rai, V. R. Devaraj","doi":"10.5505/TJB.2014.20982","DOIUrl":"https://doi.org/10.5505/TJB.2014.20982","url":null,"abstract":"Objective: In this study, we report the role of miRNAs involved under nitrogen starvation from widely grown vegetable crop, French bean. In recent years, a great deal of attention has been paid to the elucidation of miRNAs involved in low nitrate stress. Methods: To identify miRNAs expressed under stress, cDNA libraries were analyzed. Results: We reported the nine potential miRNAs with 67 targets involved in nutrient transporters and other stress specific genes. Among the miRNA sequences obtained 6 sequences belong to miR172 family, one with miR169. RT-PCR analysis of expression of miR172 family was induced upon low nitrate stress while miR169 family was repressed. In addition, Pvu-SN7b and Pvu-miR16 may be new members of miRNA172 and miR169 families, respectively. Conclusion: The targets of Pvu-SN7b were major protein kinases, one among which is the Protein Kinase CK2. CK2 Kinase is found to involve in transcription-directed signaling, gene control and cell-cycle regulation. Other targets of Pvu-SN7b were involved in DNA-dependent transcription regulation, photo-periodism, calcium-mediated signaling. Pvu-miR16 targets Thymidine kinase, the key enzyme of deoxy-nucleotide synthesis. The cleavage of these targets affects cell proliferation there by affecting nodule formation. Pvu-miR8 inhibits translation of its target protein Pre-protein translocase, a membrane-bound protein transporter involved in trans-membrane protein transportation. Together these results denote the response and role of miRNAs to nitrate-limiting conditions in French bean.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78464893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SERUM LIPIDS AND Lp-PLA2 ACTIVITY IN MULTIPLE SCLEROSIS","authors":"H. Doğan","doi":"10.5505/TJB.2014.27147","DOIUrl":"https://doi.org/10.5505/TJB.2014.27147","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77573207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Intestinal fatty acid binding protein (IFAPB) participates in the uptake, intracellular metabolism and/or transport of long chain fatty acids. A polymorphism at codon 54 in exon 2 of FABP2 gene, which encodes for the IFAPB, exchanges an Alanine for Threonine. FABP2 gene polymorphism could modify the uptake of fatty acids, and it could correlate with risk of several diseases. In the light of the potential role of the FABP2 polymorphism, the aim of this study was to determine the frequency of the Ala54Thr FABP2 polymorphism in two Middle Eastern Arab Populations. Materials and Methods: Genotyping was investigated in 182 and 120 unrelated healthy subjects from Bahrain and Jordan, respectively. A PCR-RFLP assay was applied for determination of Ala54Thr (rs1799883) FABP2 polymorphism. Allele frequencies were calculated by direct counting. Hardy Weinberg Equilibrium was evaluated using a Chi- square goodness of fit test. Results: In the studied Bahraini subjects, 52.8% were homozygous for the Ala54/Ala54 genotype, 35.7% were heterozygous for the Ala54/Thr54 genotype and 11.5% were homozygous for the Thr54/Thr54 genotype. The gene frequencies obtained in Jordanians were: 48.3%, 43.3% and 8.4% for Ala54/Ala54; Ala54/Thr54 and Thr54/Thr54 genotypes, respectively. The frequencies of the allele Ala54 and the allele Thr54 of the FABP2 gene were found to be 0.706 and 0.294 for Bahrainis and 0.700 and 0.300 for Jordanians. These results revealed a similar population polymorphism frequency as in previous European and Arab populations' studies. Conclusion: This is the first study to investigate the population frequency of the Thr54 allele in Bahraini and Jordanian populations.
{"title":"Genotype and allele frequencies of the intestinal fatty acid binding protein-2 gene in two Arab populations","authors":"A. Salem","doi":"10.5505/TJB.2014.15870","DOIUrl":"https://doi.org/10.5505/TJB.2014.15870","url":null,"abstract":"Objective: Intestinal fatty acid binding protein (IFAPB) participates in the uptake, intracellular metabolism and/or transport of long chain fatty acids. A polymorphism at codon 54 in exon 2 of FABP2 gene, which encodes for the IFAPB, exchanges an Alanine for Threonine. FABP2 gene polymorphism could modify the uptake of fatty acids, and it could correlate with risk of several diseases. In the light of the potential role of the FABP2 polymorphism, the aim of this study was to determine the frequency of the Ala54Thr FABP2 polymorphism in two Middle Eastern Arab Populations. Materials and Methods: Genotyping was investigated in 182 and 120 unrelated healthy subjects from Bahrain and Jordan, respectively. A PCR-RFLP assay was applied for determination of Ala54Thr (rs1799883) FABP2 polymorphism. Allele frequencies were calculated by direct counting. Hardy Weinberg Equilibrium was evaluated using a Chi- square goodness of fit test. Results: In the studied Bahraini subjects, 52.8% were homozygous for the Ala54/Ala54 genotype, 35.7% were heterozygous for the Ala54/Thr54 genotype and 11.5% were homozygous for the Thr54/Thr54 genotype. The gene frequencies obtained in Jordanians were: 48.3%, 43.3% and 8.4% for Ala54/Ala54; Ala54/Thr54 and Thr54/Thr54 genotypes, respectively. The frequencies of the allele Ala54 and the allele Thr54 of the FABP2 gene were found to be 0.706 and 0.294 for Bahrainis and 0.700 and 0.300 for Jordanians. These results revealed a similar population polymorphism frequency as in previous European and Arab populations' studies. Conclusion: This is the first study to investigate the population frequency of the Thr54 allele in Bahraini and Jordanian populations.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74355433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of agilent 1100 (chromsystems) and tosoh hlc-723 g8 hplc systems in thalassemia screening","authors":"H. Ellidağ","doi":"10.5505/tjb.2015.80774","DOIUrl":"https://doi.org/10.5505/tjb.2015.80774","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75666545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, we aimed to contribute prenatal diagnosis in the Çukurova region having high prevalence of hemoglobinopathies by implementing DNA sequencing analysis for the mutations undetectable by conventional methods. Methods: Hematological parameters of two families applied to Cukurova University Prenatal Diagnosis Center were analyzed. Hb variants were screened by cellulose acetate electrophoresis and by HPLC. DNA samples of cases were isolated using automatic DNA extraction machine. Microarray, RFLP, ARMS and Gap-PCR molecular methodologies carried out for determinations of hemoglobin mutations. Unidentified mutations were resolved by means of DNA Sequence Analysis. Results: We identified a previously unreported homozygous 5 nt deletional mutation [α2 IVS-1 134.-138. nt (TGAGG)] in Turkey by DNA Sequence Analysis. When we investigated twelve cases of this family by Gap-PCR and DNA Sequence Analysis it is observed that seven cases were heterozygous for 5nt deletion, a case found to be heterozygous 20.5 kb deletional mutation and four cases were found to be having no mutation. In the other family applied for prenatal diagnosis was observed to having Hb Stanleyville II [α2 78 (EF 7) Asn→ Lys (AAC→AAA)] mutation which is also a novel mutaion in Turkey. Mutations of eleven members of this family were determined by ARMS, RFLP, Gap-PCR and DNA Sequence Analysis, resulting in two cases heterozygous Hb Stanleyville II, five cases heterozygous 3.7 kb deletion, a case with heterozygous IVS-1-110 (G→C) mutation on β globin gene and three cases do not have any mutation. Conclusion: In this study, two types of mutations observed for the first time in Turkey have been identified.
{"title":"The first observation of two alpha globin mutations in Turkey: Hb Stanleyville II and a homozygous 5nt deletion","authors":"Figen Guzelgul","doi":"10.5505/TJB.2014.31967","DOIUrl":"https://doi.org/10.5505/TJB.2014.31967","url":null,"abstract":"Objective: In this study, we aimed to contribute prenatal diagnosis in the Çukurova region having high prevalence of hemoglobinopathies by implementing DNA sequencing analysis for the mutations undetectable by conventional methods. Methods: Hematological parameters of two families applied to Cukurova University Prenatal Diagnosis Center were analyzed. Hb variants were screened by cellulose acetate electrophoresis and by HPLC. DNA samples of cases were isolated using automatic DNA extraction machine. Microarray, RFLP, ARMS and Gap-PCR molecular methodologies carried out for determinations of hemoglobin mutations. Unidentified mutations were resolved by means of DNA Sequence Analysis. Results: We identified a previously unreported homozygous 5 nt deletional mutation [α2 IVS-1 134.-138. nt (TGAGG)] in Turkey by DNA Sequence Analysis. When we investigated twelve cases of this family by Gap-PCR and DNA Sequence Analysis it is observed that seven cases were heterozygous for 5nt deletion, a case found to be heterozygous 20.5 kb deletional mutation and four cases were found to be having no mutation. In the other family applied for prenatal diagnosis was observed to having Hb Stanleyville II [α2 78 (EF 7) Asn→ Lys (AAC→AAA)] mutation which is also a novel mutaion in Turkey. Mutations of eleven members of this family were determined by ARMS, RFLP, Gap-PCR and DNA Sequence Analysis, resulting in two cases heterozygous Hb Stanleyville II, five cases heterozygous 3.7 kb deletion, a case with heterozygous IVS-1-110 (G→C) mutation on β globin gene and three cases do not have any mutation. Conclusion: In this study, two types of mutations observed for the first time in Turkey have been identified.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76472441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"C-Reactive Protein vs. High - Sensitivity C - Reactive Protein: What is the Difference?","authors":"D. Yücel","doi":"10.5505/TJB.2014.92408","DOIUrl":"https://doi.org/10.5505/TJB.2014.92408","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72846072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Nowadays, in most of industrial technology, especially biotechnological processes, use of enzymes is increasingly widespread. With respect to discovery of new enzymes, one of the most successful methods is isolation of microorganisms from natural habitats. Therefore, in this research, we aimed to isolate and characterize lipase producing yeasts and purify the extracellular lipase produced by a novel yeast strain Candida odintsovae TY42. Methods: The yeasts used in the study were isolated from olive oils and identified based on sequence analyses of ITS1-5.8S rRNA-ITS2 and D1/D2 domain of 26S rRNA. The optimal nutritional and physicochemical conditions were determined by investigating the effects of initial pH and temperature and different carbon sources on enzyme production in basal medium. The lipase was purified by ammonium sulfate and acetone precipitation and anion exchange chromatography. Results: Three lipase producing yeast isolates were obtained from olive oils produced in Aegean region. The sequencing of rRNA regions revealed that novel strains, TY17, TY42 and TY54 identified as Candida odintsovae. C. odintsovae TY42 showed higher lipase activity and therefore, the yeast was selected for further investigations. The maximum lipase production was achieved in a medium containing fish oil after 144 h of incubation. The lipase was purified 12.8 fold. The molecular weight of the purified lipase was estimated to be about 39 kDa. Conclusion: This paper is the first study representing lipase production by C. odintsovae.
{"title":"Production and partial purification of extracellular lipase produced by a novel yeast strain Candida odintsovae TY42","authors":"Cengiz Çorbacı, Funda Kartal, H. T. Yalcin","doi":"10.5505/TJB.2014.78557","DOIUrl":"https://doi.org/10.5505/TJB.2014.78557","url":null,"abstract":"Objective: Nowadays, in most of industrial technology, especially biotechnological processes, use of enzymes is increasingly widespread. With respect to discovery of new enzymes, one of the most successful methods is isolation of microorganisms from natural habitats. Therefore, in this research, we aimed to isolate and characterize lipase producing yeasts and purify the extracellular lipase produced by a novel yeast strain Candida odintsovae TY42. Methods: The yeasts used in the study were isolated from olive oils and identified based on sequence analyses of ITS1-5.8S rRNA-ITS2 and D1/D2 domain of 26S rRNA. The optimal nutritional and physicochemical conditions were determined by investigating the effects of initial pH and temperature and different carbon sources on enzyme production in basal medium. The lipase was purified by ammonium sulfate and acetone precipitation and anion exchange chromatography. Results: Three lipase producing yeast isolates were obtained from olive oils produced in Aegean region. The sequencing of rRNA regions revealed that novel strains, TY17, TY42 and TY54 identified as Candida odintsovae. C. odintsovae TY42 showed higher lipase activity and therefore, the yeast was selected for further investigations. The maximum lipase production was achieved in a medium containing fish oil after 144 h of incubation. The lipase was purified 12.8 fold. The molecular weight of the purified lipase was estimated to be about 39 kDa. Conclusion: This paper is the first study representing lipase production by C. odintsovae.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81363989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), tomato leafminer, is an oligophagous insect. Larvae of T. absoluta can destroy especially tomato plants which lead to important yield loss in this economically valuable crop. Chemical control through insecticides has been a main method of controlling it in farming areas all over the world. However, continues application of certain registered insecticide such as abamectin might lead to resistance development in T. absoluta. The aim of this study was to monitor resistance status of abamectin insecticide and analyse resistance mechanisms of this insecticide in T. absoluta field populations from three districts of Turkey by using bioassay and biochemical methods. Methods: Bioassays and Biochemical assays. Results: Bioassay results showed that while Adana and Antalya strain of T. absoluta showed low resistance (3.03and 2.3-fold) to abamectin insecticide, Ankara strain of T. absoluta was not resistant to abamectin (1.31-fold). Biochemical analysis displayed that CYP450-PNOD activities showed 2.55 and 1.95-fold increase compared to susceptible population in Adana and Antalya field populations, respectively. Furthermore, GST-CDNB activities showed statistically significant (p<0.05) 1.3-fold increase only in Adana population. Although EST-α-NA activities showed 3.41-fold increase only in Ankara field population, this field population did not display a significant resistancy to abamectin. Conclusion: Consequently, cytochrome P450 monooxygenase enzymes seemed to have a major role in abamectin resistance development in field populations of T. absoluta from Turkey. In addition, GSTs possibly have supportive role such as reducing oxidative stress that developed during metabolism of abamectin in resistant field populations of T. absoluta.
{"title":"Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide","authors":"M. Konuş","doi":"10.5505/TJB.2014.09327","DOIUrl":"https://doi.org/10.5505/TJB.2014.09327","url":null,"abstract":"Objective: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), tomato leafminer, is an oligophagous insect. Larvae of T. absoluta can destroy especially tomato plants which lead to important yield loss in this economically valuable crop. Chemical control through insecticides has been a main method of controlling it in farming areas all over the world. However, continues application of certain registered insecticide such as abamectin might lead to resistance development in T. absoluta. The aim of this study was to monitor resistance status of abamectin insecticide and analyse resistance mechanisms of this insecticide in T. absoluta field populations from three districts of Turkey by using bioassay and biochemical methods. Methods: Bioassays and Biochemical assays. Results: Bioassay results showed that while Adana and Antalya strain of T. absoluta showed low resistance (3.03and 2.3-fold) to abamectin insecticide, Ankara strain of T. absoluta was not resistant to abamectin (1.31-fold). Biochemical analysis displayed that CYP450-PNOD activities showed 2.55 and 1.95-fold increase compared to susceptible population in Adana and Antalya field populations, respectively. Furthermore, GST-CDNB activities showed statistically significant (p<0.05) 1.3-fold increase only in Adana population. Although EST-α-NA activities showed 3.41-fold increase only in Ankara field population, this field population did not display a significant resistancy to abamectin. Conclusion: Consequently, cytochrome P450 monooxygenase enzymes seemed to have a major role in abamectin resistance development in field populations of T. absoluta from Turkey. In addition, GSTs possibly have supportive role such as reducing oxidative stress that developed during metabolism of abamectin in resistant field populations of T. absoluta.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91270818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Usage potential of acetylcholinesterase as a bioscavenger in organophasphate poisoning","authors":"Tuba Tuylu Kuçukkilinç","doi":"10.5505/TJB.2014.00922","DOIUrl":"https://doi.org/10.5505/TJB.2014.00922","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85083295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Glutathione S-transferases (GST) take roles under stress conditions by the conjugation of Glutathione (GSH) to electrophilic substrates to increase their solubility and facilitating further metabolic processing. This is one of the main pathways functioning in stress resistance and, for dought stress, there is not yet a study on Pinus brutia Ten. which is a very important forest tree of Turkey. Materials and Methods: The needles of 30 different individuals of Pinus brutia Ten. were collected three times in the season: at the begining, in the middle and at the end of summer (June-August). The total precipitation and the temperature of the region were surveyed during sampling period. The osmotic pressure values, total GST activities and the GSH pools of the needle samples were measured and statistically analysed. Results: The osmotic pressure values showed 16% increase throughout the season. Moreover, total precipitation and temperature values demonstrated that the highest drought stress was observed at the end of sampling period. Approximately 80% of increase in total GST activity between the beginning and the end of summer probably indicated the response against drought; although there was no significant change in the GSH pool. Conclusion: The presence of drought, elevated osmotic pressure and a satistically significant (p<0.005) increase in total GST activities in needle samples were detected. The lack of direct correlation between the GSH pool and total GST activities was explained by the rate of GSH biosynthesis and its redox cycling.
{"title":"Glutathione S-Transferase activities and glutathione levels in needles of drought stressed Pinus brutia Ten. trees","authors":"C. Yılmaz, M. Işcan","doi":"10.5505/TJB.2014.88319","DOIUrl":"https://doi.org/10.5505/TJB.2014.88319","url":null,"abstract":"Aim: Glutathione S-transferases (GST) take roles under stress conditions by the conjugation of Glutathione (GSH) to electrophilic substrates to increase their solubility and facilitating further metabolic processing. This is one of the main pathways functioning in stress resistance and, for dought stress, there is not yet a study on Pinus brutia Ten. which is a very important forest tree of Turkey. Materials and Methods: The needles of 30 different individuals of Pinus brutia Ten. were collected three times in the season: at the begining, in the middle and at the end of summer (June-August). The total precipitation and the temperature of the region were surveyed during sampling period. The osmotic pressure values, total GST activities and the GSH pools of the needle samples were measured and statistically analysed. Results: The osmotic pressure values showed 16% increase throughout the season. Moreover, total precipitation and temperature values demonstrated that the highest drought stress was observed at the end of sampling period. Approximately 80% of increase in total GST activity between the beginning and the end of summer probably indicated the response against drought; although there was no significant change in the GSH pool. Conclusion: The presence of drought, elevated osmotic pressure and a satistically significant (p<0.005) increase in total GST activities in needle samples were detected. The lack of direct correlation between the GSH pool and total GST activities was explained by the rate of GSH biosynthesis and its redox cycling.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87863900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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