首页 > 最新文献

Turkish Journal of Pharmaceutical Sciences最新文献

英文 中文
Smartphone Digital Image Colorimetry for the Determination of Aluminum in Antiperspirant Products. 智能手机数字图像比色法测定止汗产品中铝的含量。
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-12-21 DOI: 10.4274/tjps.galenos.2021.18828
Suad Abughrin, Usama Alshana, Jude Caleb

Objectives: This study aims to present a method for the determination of the aluminum in antiperspirant products (APPs) by chelating it with quercetin before its detection by smartphone digital image colorimetry (SDIC).

Materials and methods: Samples were prepared by closed-vessel acid digestion in PTFE cups. This was followed by complexation of aluminum in the sample solution using quercetin as a chelating agent. Sample solutions were transferred into a quartz ultraviolet/visible detection microcuvette for detection in a homemade colorimetric box designed for capturing images of the yellow complex with a smartphone camera. The pixel intensity of the images was converted to numbers for quantitation using ImageJ software for a personal computer. An independent study using high-performance liquid chromatography-diode-array detection was conducted to check the accuracy of the proposed method.

Results: Optimum SDIC conditions included a Samsung C9 smartphone as the detection camera, a cropped region of interest of 6400 px2, and the side position of the colorimetric box were selected for capturing the images of the sample solutions placed 10.0 cm from the detection camera, whereas optimum complexation conditions were found to be as sample pH of 5.5, sample volume of 3.0 mL, complexation time of 1.0 min and a ligand concentration of 0.28 mmol L-1. Analytical performance of the method included a limit of detection of 0.5 μmol L-1 and a coefficient of determination (R2) of the calibration graph of 0.9981.

Conclusion: The proposed method was successfully applied for the determination of aluminum in APPs with percentage recoveries ranging from 80.0 to 109.6%.

目的:建立智能手机数字图像比色法(SDIC)检测止汗产品(app)中铝含量前与槲皮素螯合的测定方法。材料和方法:在聚四氟乙烯杯中封闭酸消解制备样品。然后用槲皮素作为螯合剂使铝在样品溶液中络合。将样品溶液转移到石英紫外/可见光检测微比皿中,在自制的比色盒中进行检测,该比色盒设计用于用智能手机相机捕获黄色复合物的图像。使用个人电脑ImageJ软件将图像的像素强度转换为数字进行定量。采用高效液相色谱-二极管阵列检测进行了独立研究,以验证所提出方法的准确性。结果:以三星C9智能手机为检测相机,选择感兴趣区域面积为6400 px2,比色盒侧边位置为最优条件,对距离检测相机10.0 cm的样品溶液进行图像采集,最佳络合条件为样品pH为5.5,样品体积为3.0 mL,络合时间为1.0 min,配体浓度为0.28 mmol L-1。方法的检出限为0.5 μmol L-1,校正图的决定系数(R2)为0.9981。结论:该方法可用于app中铝的测定,回收率为80.0 ~ 109.6%。
{"title":"Smartphone Digital Image Colorimetry for the Determination of Aluminum in Antiperspirant Products.","authors":"Suad Abughrin,&nbsp;Usama Alshana,&nbsp;Jude Caleb","doi":"10.4274/tjps.galenos.2021.18828","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.18828","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to present a method for the determination of the aluminum in antiperspirant products (APPs) by chelating it with quercetin before its detection by smartphone digital image colorimetry (SDIC).</p><p><strong>Materials and methods: </strong>Samples were prepared by closed-vessel acid digestion in PTFE cups. This was followed by complexation of aluminum in the sample solution using quercetin as a chelating agent. Sample solutions were transferred into a quartz ultraviolet/visible detection microcuvette for detection in a homemade colorimetric box designed for capturing images of the yellow complex with a smartphone camera. The pixel intensity of the images was converted to numbers for quantitation using ImageJ software for a personal computer. An independent study using high-performance liquid chromatography-diode-array detection was conducted to check the accuracy of the proposed method.</p><p><strong>Results: </strong>Optimum SDIC conditions included a Samsung C9 smartphone as the detection camera, a cropped region of interest of 6400 px<sup>2</sup>, and the side position of the colorimetric box were selected for capturing the images of the sample solutions placed 10.0 cm from the detection camera, whereas optimum complexation conditions were found to be as sample pH of 5.5, sample volume of 3.0 mL, complexation time of 1.0 min and a ligand concentration of 0.28 mmol L<sup>-1</sup>. Analytical performance of the method included a limit of detection of 0.5 μmol L<sup>-1</sup> and a coefficient of determination (R<sup>2</sup>) of the calibration graph of 0.9981.</p><p><strong>Conclusion: </strong>The proposed method was successfully applied for the determination of aluminum in APPs with percentage recoveries ranging from 80.0 to 109.6%.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 6","pages":"618-625"},"PeriodicalIF":1.7,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780579/pdf/TJPS-19-618.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10799614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of Lonicera etrusca var. etrusca Santi (Caprifoliaceae) Stem and Leaf in Terms of Anatomical Structures and Some Phenolic Compounds. 从解剖结构和某些酚类化合物的角度评价刺槐科金银花的茎叶。
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-12-21 DOI: 10.4274/tjps.galenos.2021.71636
Derya Çiçek Polat, Muhammed Mesud Hürkul

Objectives: The genus Lonicera includes medicinally important plants. Two varieties of L. etrusca have been recorded in Türkiye. Anatomical structures and phytochemical contents are important in the diagnosis and identification of medicinal plants. This study included stem and leaf anatomy of L. etrusca var. etrusca and high performance liquid chromatography (HPLC) analysis of the methanol extracts obtained from these parts.

Materials and methods: Plant materials were collected from Ankara. Methanol extracts were prepared from the stems and leaves by ultrasonic bath. The amounts of chlorogenic acid and caffeic acid that are major compounds in the stem and leaves, were determined by HPLC. For anatomical studies, specimens were preserved in 70% alcohol. Transverse and surface sections were prepared by hand. Detection of tissues was performed using Sartur reagent. Anatomical specimens were examined using a light microscope and microphotographed.

Results: In HPLC analysis, the highest amount of chlorogenic acid was determined in the leaf (1.148%), and the highest amount of caffeic acid (0.156%) was determined in the stem. In the anatomical analysis, it was observed that the stem was disc-shaped and hollow; pericycle is in a ring form, consists of fibre-like cells with thick walls and wide lumina; cork occurs adjoining pericyclic fibers; thin-walled pith cells containing dense druse crystals. The leaf lamina is bifacial in the transverse section; palisade and spongy parenchyma, both contain abundant starch grains; solitary druse crystals are sparse in the leaf mesophyll; the stomata were observed only on the lower surface with 3-5 subsidiary cells. With this study, L. etrusca var. etrusca has been clarified in terms of its anatomical structures and phenolic compounds.

Conclusion: The chemical contents and anatomical structures of the plant may contain important information that can be used in classification. This study may support in taxonomically classification for the L. etrusca var. etrusca.

目的:金银花属是一种重要的药用植物。在 rkiye已记录到两个品种的L. etrusca。解剖结构和植物化学成分对药用植物的诊断和鉴定具有重要意义。本研究对伊特鲁卡的茎叶进行了解剖,并对其甲醇提取物进行了高效液相色谱分析。材料和方法:在安卡拉采集植物材料。采用超声波浴法制备甲醇提取物。采用高效液相色谱法测定了绿原酸和咖啡酸这两种主要化合物在茎叶中的含量。为了进行解剖学研究,标本保存在70%的酒精中。横向和表面切片手工制作。组织检测采用Sartur试剂。解剖标本采用光镜检查和显微照相。结果:在HPLC分析中,叶中绿原酸含量最高(1.148%),茎中咖啡酸含量最高(0.156%)。在解剖分析中,观察到茎呈盘状,中空;中柱鞘呈环状,由纤维样细胞组成,壁厚,腔宽;栓皮发生在邻近的周环纤维;含有致密晶体的薄壁髓细胞。叶片横切面为双面;栅栏和海绵状薄壁组织均含有丰富的淀粉粒;叶肉中稀疏的单生絮状晶体;气孔仅下表面有3-5个附属细胞。本研究从解剖结构和酚类成分等方面对木香进行了初步的研究。结论:该植物的化学成分和解剖结构可能包含重要的信息,可用于分类。本研究可为松茸的分类提供依据。
{"title":"Evaluation of <i>Lonicera etrusca</i> var. <i>etrusca</i> Santi (Caprifoliaceae) Stem and Leaf in Terms of Anatomical Structures and Some Phenolic Compounds.","authors":"Derya Çiçek Polat,&nbsp;Muhammed Mesud Hürkul","doi":"10.4274/tjps.galenos.2021.71636","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.71636","url":null,"abstract":"<p><strong>Objectives: </strong>The genus <i>Lonicera</i> includes medicinally important plants. Two varieties of <i>L. etrusca</i> have been recorded in Türkiye. Anatomical structures and phytochemical contents are important in the diagnosis and identification of medicinal plants. This study included stem and leaf anatomy of <i>L. etrusca</i> var. etrusca and high performance liquid chromatography (HPLC) analysis of the methanol extracts obtained from these parts.</p><p><strong>Materials and methods: </strong>Plant materials were collected from Ankara. Methanol extracts were prepared from the stems and leaves by ultrasonic bath. The amounts of chlorogenic acid and caffeic acid that are major compounds in the stem and leaves, were determined by HPLC. For anatomical studies, specimens were preserved in 70% alcohol. Transverse and surface sections were prepared by hand. Detection of tissues was performed using Sartur reagent. Anatomical specimens were examined using a light microscope and microphotographed.</p><p><strong>Results: </strong>In HPLC analysis, the highest amount of chlorogenic acid was determined in the leaf (1.148%), and the highest amount of caffeic acid (0.156%) was determined in the stem. In the anatomical analysis, it was observed that the stem was disc-shaped and hollow; pericycle is in a ring form, consists of fibre-like cells with thick walls and wide lumina; cork occurs adjoining pericyclic fibers; thin-walled pith cells containing dense druse crystals. The leaf lamina is bifacial in the transverse section; palisade and spongy parenchyma, both contain abundant starch grains; solitary druse crystals are sparse in the leaf mesophyll; the stomata were observed only on the lower surface with 3-5 subsidiary cells. With this study, <i>L. etrusca</i> var. <i>etrusca</i> has been clarified in terms of its anatomical structures and phenolic compounds.</p><p><strong>Conclusion: </strong>The chemical contents and anatomical structures of the plant may contain important information that can be used in classification. This study may support in taxonomically classification for the <i>L. etrusca</i> var. etrusca.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 6","pages":"636-641"},"PeriodicalIF":1.7,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780581/pdf/TJPS-19-636.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10509336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Determination of Factors Influencing Pharmacists While Recommending Immune-Enhancing Products via Analytic Hierarchy Process. 运用层次分析法确定药师推荐免疫增强产品的影响因素。
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-12-21 DOI: 10.4274/tjps.galenos.2022.02686
Nilay Tarhan, Miray Arslan

Objectives: Immune enhancers are attracting attention day by day. Besides, during the coronavirus disease-2019 (COVID-19) pandemic, there has been an increasing demand for immune enhancers. Pharmacists are seen as trustable providers of complementary and alternative medicines, dietary and herbal supplements, immune-enhancers, and so on. This study aims to prioritization criteria that affect community pharmacists' recommending behavior regarding immune enhancers.

Materials and methods: This paper adopts the analytic hierarchy process (AHP) to rank different criteria substantial for affecting community pharmacists' recommending behavior regarding immune enhancers. In this direction, firstly seven criteria were identified through literature review and views of pharmacists who have community pharmacy experiences. These are; (i) ease of access, (ii) selling price, (iii) package, (iv) content (appropriateness to patient health status), (v) expectation of patient, (vi) quality, and (vii) trust in the manufacturer. Then, a questionnaire including criteria was prepared and delivered to community pharmacists. The data obtained from 93 participants were transferred to the Super Decisions software. The hierarchical structure of the AHP was established and pair-wise comparisons were made.

Results: This study showed that the most important criterion was the ease of access (28%). Secondly, pharmacists give importance to the content of the product, while advising immune-enhancers (22%). Besides, it was determined that the least important criterion was the package of the product (4%).

Conclusion: This study will contribute to the literature by facilitating the process of assessing factors that pharmacists pay attention to while recommending immune-enhancing products. Additionally, the present study results will shed light on firms producing such products, to shape their supply chain management strategies, especially for marketing and sales.

目的:免疫增强剂日益引起人们的注意。此外,在2019冠状病毒病(COVID-19)大流行期间,对免疫增强剂的需求不断增加。药剂师被视为补充和替代药物、膳食和草药补充剂、免疫增强剂等的可靠提供者。本研究旨在确定影响社区药剂师推荐免疫增强剂行为的优先标准。材料与方法:采用层次分析法(AHP)对影响社区药师推荐免疫增强剂行为的不同标准进行排序。在此方向上,首先通过文献综述和具有社区药房经验的药剂师的观点,确定了七个标准。这些都是;(i)获取便利程度,(ii)售价,(iii)包装,(iv)内容(适合患者健康状况),(v)患者期望,(vi)质量,以及(vii)对制造商的信任。然后,准备了一份包括标准的调查问卷,并分发给社区药剂师。从93名参与者那里获得的数据被转移到超级决策软件中。建立层次分析法的层次结构,并进行两两比较。结果:本研究显示,最重要的标准是可及性(28%)。其次,药剂师重视产品的含量,同时建议使用免疫增强剂(22%)。此外,确定最不重要的标准是产品的包装(4%)。结论:本研究促进了药师在推荐免疫增强产品时应注意的因素的评估过程,有助于文献的完善。此外,目前的研究结果将阐明生产此类产品的公司,以形成其供应链管理战略,特别是营销和销售。
{"title":"Determination of Factors Influencing Pharmacists While Recommending Immune-Enhancing Products <i>via</i> Analytic Hierarchy Process.","authors":"Nilay Tarhan,&nbsp;Miray Arslan","doi":"10.4274/tjps.galenos.2022.02686","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.02686","url":null,"abstract":"<p><strong>Objectives: </strong>Immune enhancers are attracting attention day by day. Besides, during the coronavirus disease-2019 (COVID-19) pandemic, there has been an increasing demand for immune enhancers. Pharmacists are seen as trustable providers of complementary and alternative medicines, dietary and herbal supplements, immune-enhancers, and so on. This study aims to prioritization criteria that affect community pharmacists' recommending behavior regarding immune enhancers.</p><p><strong>Materials and methods: </strong>This paper adopts the analytic hierarchy process (AHP) to rank different criteria substantial for affecting community pharmacists' recommending behavior regarding immune enhancers. In this direction, firstly seven criteria were identified through literature review and views of pharmacists who have community pharmacy experiences. These are; (i) ease of access, (ii) selling price, (iii) package, (iv) content (appropriateness to patient health status), (v) expectation of patient, (vi) quality, and (vii) trust in the manufacturer. Then, a questionnaire including criteria was prepared and delivered to community pharmacists. The data obtained from 93 participants were transferred to the Super Decisions software. The hierarchical structure of the AHP was established and pair-wise comparisons were made.</p><p><strong>Results: </strong>This study showed that the most important criterion was the ease of access (28%). Secondly, pharmacists give importance to the content of the product, while advising immune-enhancers (22%). Besides, it was determined that the least important criterion was the package of the product (4%).</p><p><strong>Conclusion: </strong>This study will contribute to the literature by facilitating the process of assessing factors that pharmacists pay attention to while recommending immune-enhancing products. Additionally, the present study results will shed light on firms producing such products, to shape their supply chain management strategies, especially for marketing and sales.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 6","pages":"701-705"},"PeriodicalIF":1.7,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780582/pdf/TJPS-19-701.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10452331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Peptide Sequence of Pili Subunit Protein 49.8 kDa Shigella flexneri as Antigenic Epitope for Shigellosis Vaccine Development. 志贺氏菌菌毛亚基蛋白49.8 kDa作为志贺氏菌疫苗抗原表位的肽序列
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-12-21 DOI: 10.4274/tjps.galenos.2021.75031
Khoirul Anam, Agustina Tri Endharti, Sri Poeranto, Sumarno Reto Prawiro

Objectives: This study investigates the amino acid sequence and identifies antigenic epitopes of 49.8 kilodalton (kDa) pili protein Shigella flexneri, which will be used as candidates for the shigellosis vaccine.

Materials and methods: Our study is a prospectively descriptive laboratory. We used bacterial isolate of S. flexneri pili isolation was performed using a pili cutter and sodium dodecyl-sulfate polyacrylamide gel electrophoresis. The amino acid sequences were analyzed using liquid chromatography dual mass spectrometry (LC-MS/MS) method in the proteomic laboratory. The target epitope antigenicity analysis was tested using Kolaskar and Tongaonkar Antigenicity software. The Bepired Linear Epitope Prediction software is used for epitope mapping. PymOL software was used for the visualization of proteins and molecular docking. Peptides and antibodies were applied to hemagglutination test and immune response was tested using the dot blot method.

Results: LC-MS/MS analysis results from the mascot server showed that the 49.8 kDa pili protein is S. flexneri similar to the flagellin protein of S. flexneri 1235-66 (ID I6H2T2). The results of antigenicity analysis and epitope mapping showed that areas of protein that has the most potential and antigenic epitopes are the regions 98-111 and 263-290 with the amino acid sequences, QSSTGTNSQSDLDS (Q-S) and DTTITKAETKTVTKNQVVDTPVTTDAAK (D-K). The results of the molecular docking interaction test between the peptide and the B-cell receptor have a low binding energy. Peptide Q-S and peptide D-K antigens are hemagglutinin molecules because they can agglutinate erythrocytes. The immune response between peptide antigens and anti-peptide antibodies can react based on color gradations in the dotblot method.

Conclusion: The amino acid sequences Q-S and D-K are potentially antigenic epitopes. These peptides can be used to develop candidates for shigellosis vaccine.

目的:研究49.8千道尔顿(kDa)佛氏志贺氏菌菌毛蛋白的氨基酸序列,并鉴定其抗原表位,为志贺氏菌病疫苗的候选物提供依据。材料和方法:我们的研究是前瞻性描述性实验室。本研究采用菌群分离法对flexneri菌毛进行分离,并用毛切割器和十二烷基硫酸钠聚丙烯酰胺凝胶电泳进行分离。氨基酸序列在蛋白质组学实验室采用液相色谱双质谱法(LC-MS/MS)进行分析。目的表位抗原性分析采用Kolaskar和Tongaonkar antigenicity软件进行。Bepired线性表位预测软件用于表位定位。使用PymOL软件对蛋白质进行可视化和分子对接。用多肽和抗体进行血凝试验,用点印迹法检测免疫应答。结果:从mascot server中提取的49.8 kDa菌毛蛋白与S. flexneri 1235-66 (ID I6H2T2)的鞭毛蛋白相似,为flexneri。抗原性分析和表位定位结果显示,氨基酸序列为QSSTGTNSQSDLDS (Q-S)和DTTITKAETKTVTKNQVVDTPVTTDAAK (D-K)的98-111和263-290的蛋白区最有潜力和抗原表位。肽与b细胞受体的分子对接相互作用试验结果显示其结合能较低。肽Q-S和肽D-K抗原是血凝素分子,因为它们能凝集红细胞。在斑点斑点法中,肽抗原和抗肽抗体之间的免疫反应可以根据颜色的渐变进行反应。结论:Q-S和D-K氨基酸序列是潜在的抗原表位。这些肽可用于开发志贺氏菌病候选疫苗。
{"title":"Peptide Sequence of Pili Subunit Protein 49.8 kDa <i>Shigella flexneri</i> as Antigenic Epitope for Shigellosis Vaccine Development.","authors":"Khoirul Anam,&nbsp;Agustina Tri Endharti,&nbsp;Sri Poeranto,&nbsp;Sumarno Reto Prawiro","doi":"10.4274/tjps.galenos.2021.75031","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.75031","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigates the amino acid sequence and identifies antigenic epitopes of 49.8 kilodalton (kDa) pili protein <i>Shigella flexner</i>i, which will be used as candidates for the shigellosis vaccine.</p><p><strong>Materials and methods: </strong>Our study is a prospectively descriptive laboratory. We used bacterial isolate of <i>S. flexneri</i> pili isolation was performed using a pili cutter and sodium dodecyl-sulfate polyacrylamide gel electrophoresis. The amino acid sequences were analyzed using liquid chromatography dual mass spectrometry (LC-MS/MS) method in the proteomic laboratory. The target epitope antigenicity analysis was tested using Kolaskar and Tongaonkar Antigenicity software. The Bepired Linear Epitope Prediction software is used for epitope mapping. PymOL software was used for the visualization of proteins and molecular docking. Peptides and antibodies were applied to hemagglutination test and immune response was tested using the dot blot method.</p><p><strong>Results: </strong>LC-MS/MS analysis results from the mascot server showed that the 49.8 kDa pili protein is <i>S. flexneri</i> similar to the flagellin protein of <i>S. flexneri</i> 1235-66 (ID I6H2T2). The results of antigenicity analysis and epitope mapping showed that areas of protein that has the most potential and antigenic epitopes are the regions 98-111 and 263-290 with the amino acid sequences, <i>QSSTGTNSQSDLDS</i> (Q-S) and <i>DTTITKAETKTVTKNQVVDTPVTTDAAK</i> (D-K). The results of the molecular docking interaction test between the peptide and the B-cell receptor have a low binding energy. Peptide Q-S and peptide D-K antigens are hemagglutinin molecules because they can agglutinate erythrocytes. The immune response between peptide antigens and anti-peptide antibodies can react based on color gradations in the dotblot method.</p><p><strong>Conclusion: </strong>The amino acid sequences Q-S and D-K are potentially antigenic epitopes. These peptides can be used to develop candidates for shigellosis vaccine.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 6","pages":"649-656"},"PeriodicalIF":1.7,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780573/pdf/TJPS-19-649.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10509338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Biodegredable Microparticles for Mucosal Vaccination Against Diphtheria Toxoid: Nasal Efficacy Studies in Guinea Pigs 生物可降解微粒用于白喉类毒素粘膜疫苗接种的评价:豚鼠鼻腔疗效研究
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-12-14 DOI: 10.4274/tjps.galenos.2022.05626
Selin Çoban, O. M. Saka, A. Bozkır
Introduction: In this study, Poly-(ɛ-caprolactone) (PCL) and Poly-(lactic-co-glycolic acid) (PLGA) microparticles encapsulating diphtheria toxoid (DT) were investigated for their potential as a mucosal vaccine delivery system. Materials and Methods: The antigen-containing microparticles were prepared using double emulsion (w/o/w) solvent evaporation method. Results: The average geometric diameter of the particles were found between 7 and 24 µm which is suitable for uptake by the antigen presenting cells in the nasal mucosa. Although the differences were not significant, PLGA polymer containing formulations exhibited the highest encapsulation efficiency. The microparticle formulations, prepared with both PLGA and PCL polymers, was successfully produced at high production yields. The in vitro release profile was presented as a biexponential process with an initial burst effect due to the release of the protein adsorbed on the microsphere surface and the subsequent sustained release profile is the result of protein diffusion through the channels or pores formed in the polymer matrix. DT loaded microparticles, DT solution in phosphate buffered saline and empty microparticles (as control) were administered via nasal route and subcutaneously to guinea pigs. The antibody content of each serum sample was determined by an enzyme-linked immunosorbent assay. Conclusion: Absorbance values of ELISA test showed that PLGA and PCL bearing microparticles were able to stimulate adequate systemic immune response with intranasal vaccination. Additionally, PLGA and PCL microparticles resulted in significantly increased IgG titers with intranasal administration as a booster dose following subcutaneous administration. PCL polymer elicited a high immune response compared to PLGA polymer (p<0,05).
本研究研究了包封白喉类毒素(DT)的聚己内酯(PCL)和聚乳酸-羟基乙酸(PLGA)微颗粒作为粘膜疫苗递送系统的潜力。材料与方法:采用双乳液(w/o/w)溶剂蒸发法制备抗原微粒。结果:颗粒的平均几何直径在7 ~ 24µm之间,适合于鼻黏膜抗原提呈细胞的摄取。虽然差异不显著,但含PLGA聚合物的配方表现出最高的包封效率。用PLGA和PCL聚合物制备的微粒配方,以高产量成功生产。体外释放是一个双指数过程,最初是由于蛋白质吸附在微球表面释放而产生的爆发效应,随后是蛋白质通过聚合物基质中形成的通道或孔扩散的结果。载DT微颗粒、磷酸盐缓冲盐水中的DT溶液和空微颗粒(作为对照)通过鼻路和皮下给药给药。每个血清样品的抗体含量采用酶联免疫吸附法测定。结论:酶联免疫吸附试验的吸光度值表明,含PLGA和PCL的微颗粒在鼻内接种时能够激发足够的全身免疫反应。此外,PLGA和PCL微粒在皮下给药后,鼻内给药可显著增加IgG滴度。与PLGA聚合物相比,PCL聚合物引起了更高的免疫应答(p< 0.05)。
{"title":"Evaluation of Biodegredable Microparticles for Mucosal Vaccination Against Diphtheria Toxoid: Nasal Efficacy Studies in Guinea Pigs","authors":"Selin Çoban, O. M. Saka, A. Bozkır","doi":"10.4274/tjps.galenos.2022.05626","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.05626","url":null,"abstract":"Introduction: In this study, Poly-(ɛ-caprolactone) (PCL) and Poly-(lactic-co-glycolic acid) (PLGA) microparticles encapsulating diphtheria toxoid (DT) were investigated for their potential as a mucosal vaccine delivery system. Materials and Methods: The antigen-containing microparticles were prepared using double emulsion (w/o/w) solvent evaporation method. Results: The average geometric diameter of the particles were found between 7 and 24 µm which is suitable for uptake by the antigen presenting cells in the nasal mucosa. Although the differences were not significant, PLGA polymer containing formulations exhibited the highest encapsulation efficiency. The microparticle formulations, prepared with both PLGA and PCL polymers, was successfully produced at high production yields. The in vitro release profile was presented as a biexponential process with an initial burst effect due to the release of the protein adsorbed on the microsphere surface and the subsequent sustained release profile is the result of protein diffusion through the channels or pores formed in the polymer matrix. DT loaded microparticles, DT solution in phosphate buffered saline and empty microparticles (as control) were administered via nasal route and subcutaneously to guinea pigs. The antibody content of each serum sample was determined by an enzyme-linked immunosorbent assay. Conclusion: Absorbance values of ELISA test showed that PLGA and PCL bearing microparticles were able to stimulate adequate systemic immune response with intranasal vaccination. Additionally, PLGA and PCL microparticles resulted in significantly increased IgG titers with intranasal administration as a booster dose following subcutaneous administration. PCL polymer elicited a high immune response compared to PLGA polymer (p<0,05).","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42708173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Forskolin Microemulsion Formula and its Irritation Test on Rabbits 毛喉素微乳制剂的研制及其对家兔的刺激性试验
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-12-01 DOI: 10.4274/tjps.galenos.2022.73373
Rahma Nafiah, Y. Sumirtapura, S. T. Darijanto, M. Iwo
Objective: This study aims to develop a microemulsion formula that can increase the solubility and stability of forskolin and is safe for topical use. Materials and Methods: The materials used for the development of the microemulsion formula were triglyceride oil, nonionic surfactants, and polyethylene glycol for cosurfactants which were selected based on the results of the forskolin solubility test using high performance liquid chromatography. Microemulsion was formulated by phase titration method. Formula stability was determined on storage for 90 days at refrigerator, room temperature
目的:本研究旨在开发一种能提高毛喉素溶解度和稳定性、局部使用安全的微乳液配方。材料和方法:根据高效液相色谱法测定毛喉素溶解度的结果,选择甘油三酯油、非离子表面活性剂和聚乙二醇作为助表面活性剂,开发微乳剂配方。采用相滴定法配制微乳液。在冰箱、室温下储存90天后测定配方的稳定性
{"title":"Development of Forskolin Microemulsion Formula and its Irritation Test on Rabbits","authors":"Rahma Nafiah, Y. Sumirtapura, S. T. Darijanto, M. Iwo","doi":"10.4274/tjps.galenos.2022.73373","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.73373","url":null,"abstract":"Objective: This study aims to develop a microemulsion formula that can increase the solubility and stability of forskolin and is safe for topical use. Materials and Methods: The materials used for the development of the microemulsion formula were triglyceride oil, nonionic surfactants, and polyethylene glycol for cosurfactants which were selected based on the results of the forskolin solubility test using high performance liquid chromatography. Microemulsion was formulated by phase titration method. Formula stability was determined on storage for 90 days at refrigerator, room temperature","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43304707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Importance of Project-based learning (PBL) for Pharmacy Education 项目学习对药学教育的重要性
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-11-08 DOI: 10.4274/tjps.galenos.2022.69023
K. Ashiq
Project-based learning (PBL) is defined as a question-based teaching approach that ensures the active participation of the learners in building knowledge by enabling them to carry out meaningful projects and develop concrete products. Project-based learning (PBL) is generally regarded as an alternative to the traditional teaching provided by teachers. It is recognized that project-based learning (PBL) is a favorable approach that enhances student learning in higher education. 1 Project-based learning (PBL) was incorporated into educational practice in early 1980s. The history of project-based learning (PBL) has its roots in the progressive tradition encouraged by John Dewey. He emphasized the concept of "learning through practice". John Dewey maintained that the lecture room should be a sort of society and in the learning process the focus should be on the students. Project-based learning (PBL) is a highly efficient technique that allows students; to express their views on topics that cover areas of their interest, to raise their queries
基于项目的学习(PBL)被定义为一种基于问题的教学方法,通过使学习者能够执行有意义的项目和开发具体的产品,确保他们积极参与构建知识。基于项目的学习(PBL)通常被认为是教师提供的传统教学的替代方案。基于项目的学习(PBL)是一种在高等教育中促进学生学习的有利方法。1项目学习(PBL)在20世纪80年代初被纳入教育实践。基于项目的学习(PBL)的历史植根于约翰·杜威倡导的进步传统。他强调了“在实践中学习”的概念。约翰·杜威认为,演讲室应该是一种社会,在学习过程中应该以学生为中心。基于项目的学习(PBL)是一种高效的技术,它允许学生;就他们感兴趣的领域发表意见,提出疑问
{"title":"Importance of Project-based learning (PBL) for Pharmacy Education","authors":"K. Ashiq","doi":"10.4274/tjps.galenos.2022.69023","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2022.69023","url":null,"abstract":"Project-based learning (PBL) is defined as a question-based teaching approach that ensures the active participation of the learners in building knowledge by enabling them to carry out meaningful projects and develop concrete products. Project-based learning (PBL) is generally regarded as an alternative to the traditional teaching provided by teachers. It is recognized that project-based learning (PBL) is a favorable approach that enhances student learning in higher education. 1 Project-based learning (PBL) was incorporated into educational practice in early 1980s. The history of project-based learning (PBL) has its roots in the progressive tradition encouraged by John Dewey. He emphasized the concept of \"learning through practice\". John Dewey maintained that the lecture room should be a sort of society and in the learning process the focus should be on the students. Project-based learning (PBL) is a highly efficient technique that allows students; to express their views on topics that cover areas of their interest, to raise their queries","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47081799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Stability Indicating Assay Method for the Quantitative Determination of Olaparib in Bulk and Pharmaceutical Dosage Form. 原料药和制剂奥拉帕尼定量测定的稳定性指示法。
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-10-31 DOI: 10.4274/tjps.galenos.2021.48861
Antima Chaudhary, Rajiv Tonk, Pankaj Dagur, Suddhasattya Dey, Manik Ghosh

Objectives: Olaparib is an orally active poly (ADP-ribose) PARP (polymerases) inhibitor known to destroy cancer cells with BRCA1 or BRCA2 deficiency. An authentic, fast, distinct, and reliable reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and promptly validated in tablet formulations for olaparib estimation.

Materials and methods: The proposed method focuses on the separation of olaparib in reverse phase mode using a Waters symmetry C18 (150 x 4.6 mm, 5 μm) analytical column with a flow rate of 1.0 mL/min and the injection volume was kept at 20 μL. The optimized mobile phase consists of ammonium acetate buffer (pH adjusted to 3.5 by glacial acetic acid): methanol in the ratio of 50:50 v/v.

Results: The eluents were measured at 254 nm and the retention time for the drug encircled was about 4.32 min. The stress degradation studies of olaparib were conducted under acidic, alkaline, oxidative, photolytic and thermal conditions to demonstrate the stability of the drug. The regression value of 0.998 showed that the developed method was linear over the range of 80 μg/mL to 120 μg/mL. The developed RP-HPLC method is accurate and precise. The method was statistically validated as per International Conference on Harmonization guidelines.

Conclusion: The proposed method is suitable and can be applied for the quantitative estimation of olaparib without any interference of the excipients used in the drug formulations.

目的:奥拉帕尼是一种口服活性聚(adp -核糖)PARP(聚合酶)抑制剂,已知可破坏BRCA1或BRCA2缺乏症的癌细胞。建立了一种真实、快速、清晰、可靠的反相高效液相色谱(RP-HPLC)方法,并在奥拉帕尼片剂中进行了快速验证。材料和方法:采用Waters对称C18 (150 × 4.6 mm, 5 μm)色谱柱,流速为1.0 mL/min,进样量为20 μL,反相分离奥拉帕尼。优化后的流动相为醋酸铵缓冲液(冰醋酸调节pH至3.5):甲醇,比例为50:50 v/v。结果:洗脱液在254 nm处测得,环绕药物的停留时间约为4.32 min。对奥拉帕尼进行了酸性、碱性、氧化、光解和热等条件下的应力降解研究,证明了药物的稳定性。在80 ~ 120 μg/mL范围内,回归值为0.998,线性关系良好。所建立的反相高效液相色谱法准确、精密度高。根据国际协调会议的指导方针,对该方法进行了统计验证。结论:该方法适用于奥拉帕尼的定量测定,不受制剂辅料的干扰。
{"title":"Stability Indicating Assay Method for the Quantitative Determination of Olaparib in Bulk and Pharmaceutical Dosage Form.","authors":"Antima Chaudhary,&nbsp;Rajiv Tonk,&nbsp;Pankaj Dagur,&nbsp;Suddhasattya Dey,&nbsp;Manik Ghosh","doi":"10.4274/tjps.galenos.2021.48861","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.48861","url":null,"abstract":"<p><strong>Objectives: </strong>Olaparib is an orally active poly (ADP-ribose) PARP (polymerases) inhibitor known to destroy cancer cells with BRCA1 or BRCA2 deficiency. An authentic, fast, distinct, and reliable reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and promptly validated in tablet formulations for olaparib estimation.</p><p><strong>Materials and methods: </strong>The proposed method focuses on the separation of olaparib in reverse phase mode using a Waters symmetry C18 (150 x 4.6 mm, 5 μm) analytical column with a flow rate of 1.0 mL/min and the injection volume was kept at 20 μL. The optimized mobile phase consists of ammonium acetate buffer (pH adjusted to 3.5 by glacial acetic acid): methanol in the ratio of 50:50 v/v.</p><p><strong>Results: </strong>The eluents were measured at 254 nm and the retention time for the drug encircled was about 4.32 min. The stress degradation studies of olaparib were conducted under acidic, alkaline, oxidative, photolytic and thermal conditions to demonstrate the stability of the drug. The regression value of 0.998 showed that the developed method was linear over the range of 80 μg/mL to 120 μg/mL. The developed RP-HPLC method is accurate and precise. The method was statistically validated as per International Conference on Harmonization guidelines.</p><p><strong>Conclusion: </strong>The proposed method is suitable and can be applied for the quantitative estimation of olaparib without any interference of the excipients used in the drug formulations.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 5","pages":"488-497"},"PeriodicalIF":1.7,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634445/pdf/TJPS-19-488.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40659552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Tableting Performance of Maize and Potato Starches Used in Combination as Binder/Disintegrant in Metronidazole Tablet Formulation. 玉米、马铃薯淀粉复合作甲硝唑片粘结剂/崩解剂的压片性能研究
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-10-31 DOI: 10.4274/tjps.galenos.2021.47855
Yonni Eshovo Apeji, Rejoice Thomas Kaigama, Sani Hadi Ibrahim, Sophie Nock Anyebe, Aisha Ohunene Abdussalam, Avosuahi Rukayat Oyi

Objectives: This study aimed to characterize the tableting performance of maize and potato starches, when used in combination either as a disintegrant or binder in solid dosage form development.

Materials and methods: Wet granulation was used to process metronidazole granules incorporating either maize starch, potato starch, or a combination of the two starches as binders or disintegrant at 10% w/w. Granule analysis was carried out on the various formulations and subsequently compressed into tablets weighing approximately 500 mg following the addition of extragranular excipients. Tablet properties were assessed after 24 h of storage.

Results: Analysis of granule properties did not reveal a wide variation across the formulations irrespective of the type and combination of starches used in the formulation either as binder or disintegrant. It was observed, however, that there were slight differences in particle size, bulk and tapped densities of granule formulations containing the combined starch as excipients compared to granule formulations containing individual starch as the excipient. Tablets prepared using the combined starches as binder had lower tensile strength and disintegration time compared to other formulations incorporating the individual starches as binders. However, when evaluated as disintegrant, the tablet formulation containing the combined starches produced tablets with relatively lower disintegration time compared to formulations containing the individual starches as disintegrant.

Conclusion: The study concludes that the combination of maize and potato starches as excipients in tablet formulation influenced the outcome of granule and tablet properties.

目的:本研究旨在描述玉米和马铃薯淀粉在固体剂型开发中作为崩解剂或粘合剂组合使用时的压片性能。材料和方法:采用湿法造粒,以玉米淀粉、马铃薯淀粉或两种淀粉的组合为粘合剂或崩解剂,以10% w/w的速度加工甲硝唑颗粒。对各种配方进行颗粒分析,然后在添加颗粒外辅料后压缩成重约500毫克的片剂。储存24 h后评价片剂性能。结果:颗粒性质的分析并没有揭示出在配方中使用的淀粉的类型和组合有很大的变化,无论是粘合剂还是崩解剂。然而,我们观察到,含有复合淀粉作为辅料的颗粒制剂与含有单个淀粉作为辅料的颗粒制剂相比,在粒径、体积和密度上有细微的差异。使用复合淀粉作为粘结剂制备的片剂与其他采用单独淀粉作为粘结剂的制剂相比,具有较低的抗拉强度和崩解时间。然而,当被评估为崩解剂时,与含有单个淀粉作为崩解剂的配方相比,含有组合淀粉的片剂配方产生的片剂崩解时间相对较短。结论:玉米和马铃薯淀粉配作辅料对片剂的颗粒剂效果和片剂性能均有影响。
{"title":"Tableting Performance of Maize and Potato Starches Used in Combination as Binder/Disintegrant in Metronidazole Tablet Formulation.","authors":"Yonni Eshovo Apeji,&nbsp;Rejoice Thomas Kaigama,&nbsp;Sani Hadi Ibrahim,&nbsp;Sophie Nock Anyebe,&nbsp;Aisha Ohunene Abdussalam,&nbsp;Avosuahi Rukayat Oyi","doi":"10.4274/tjps.galenos.2021.47855","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.47855","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to characterize the tableting performance of maize and potato starches, when used in combination either as a disintegrant or binder in solid dosage form development.</p><p><strong>Materials and methods: </strong>Wet granulation was used to process metronidazole granules incorporating either maize starch, potato starch, or a combination of the two starches as binders or disintegrant at 10% <sup>w</sup>/<sub>w</sub>. Granule analysis was carried out on the various formulations and subsequently compressed into tablets weighing approximately 500 mg following the addition of extragranular excipients. Tablet properties were assessed after 24 h of storage.</p><p><strong>Results: </strong>Analysis of granule properties did not reveal a wide variation across the formulations irrespective of the type and combination of starches used in the formulation either as binder or disintegrant. It was observed, however, that there were slight differences in particle size, bulk and tapped densities of granule formulations containing the combined starch as excipients compared to granule formulations containing individual starch as the excipient. Tablets prepared using the combined starches as binder had lower tensile strength and disintegration time compared to other formulations incorporating the individual starches as binders. However, when evaluated as disintegrant, the tablet formulation containing the combined starches produced tablets with relatively lower disintegration time compared to formulations containing the individual starches as disintegrant.</p><p><strong>Conclusion: </strong>The study concludes that the combination of maize and potato starches as excipients in tablet formulation influenced the outcome of granule and tablet properties.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 5","pages":"513-520"},"PeriodicalIF":1.7,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634452/pdf/TJPS-19-513.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40660461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Hepcidin as a Potential Biomarker for the Diagnosis of Anemia. Hepcidin作为诊断贫血的潜在生物标志物。
IF 1.7 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2022-10-31 DOI: 10.4274/tjps.galenos.2021.29488
Zainab H Fathi, Jehan A Mohammad, Zaid M Younus, Sameer M Mahmood

There are several blood-based markers to assess iron stores, but they all have some limitations. Hepcidin, a low-molecular-weight peptide hormone, is produced mainly by the liver. It is the main regulator of iron homeostasis by preventing iron release into plasma from absorptive enterocytes and macrophages. This review aims to critically assess existing data on potential role of hepcidin in diagnosis, particularly the (pre) analytical implications of the hepcidin measurement. There is a well-known causative correlation between hepcidin and iron deficiency. Therefore, hepcidin is considered as a promising marker in the assessment of iron status, particularly in patients with a diagnostic dilemma, such as patients with chronic renal disease and infants. The clinical implications of this peptide hormone in diagnosis of other diseases have been expanded in the recent studies, including elevated hepcidin levels in neoplastic diseases, sepsis, and inflammation. The potential role of hepcidin in diagnosis is controversial in the various types of iron deficiency because data are conflicting (as in anaemia of chronic disease) or limited (as in infants), whereas in the case of hereditary haemochromatosis, it has been proposed that hepcidin may be used for stratification of molecular testing, or to improve the frequency of phlebotomy, however, this issue still needs to be investigated. Due to lack of a clinically approved test, the medical application of this peptide as a biomarker in diagnosis is restricted. Recently, assays have been developed to determine hepcidin levels in serum and urine, facilitating the future use of hepcidin in research and clinical practice.

有几种基于血液的标志物来评估铁储量,但它们都有一些局限性。肝磷脂是一种低分子量的肽激素,主要由肝脏产生。它是铁稳态的主要调节剂,通过阻止铁从吸收性肠细胞和巨噬细胞释放到血浆中。本综述旨在批判性地评估hepcidin在诊断中的潜在作用的现有数据,特别是hepcidin测量的(预)分析意义。hepcidin和缺铁之间有众所周知的因果关系。因此,hepcidin被认为是评估铁状态的一个有希望的标志物,特别是在诊断困难的患者,如慢性肾脏疾病患者和婴儿中。在最近的研究中,这种肽激素在诊断其他疾病中的临床意义已经扩大,包括肿瘤疾病、败血症和炎症中的hepcidin水平升高。hepcidin在各种类型的缺铁诊断中的潜在作用是有争议的,因为数据是相互矛盾的(如慢性病贫血)或有限的(如婴儿),而在遗传性血色素沉着病的情况下,有人提出hepcidin可能用于分子检测的分层,或提高静脉切开术的频率,然而,这个问题仍然需要调查。由于缺乏临床批准的测试,该肽作为诊断生物标志物的医学应用受到限制。最近,已经开发出测定血清和尿液中hepcidin水平的方法,促进了hepcidin在未来研究和临床实践中的应用。
{"title":"Hepcidin as a Potential Biomarker for the Diagnosis of Anemia.","authors":"Zainab H Fathi,&nbsp;Jehan A Mohammad,&nbsp;Zaid M Younus,&nbsp;Sameer M Mahmood","doi":"10.4274/tjps.galenos.2021.29488","DOIUrl":"https://doi.org/10.4274/tjps.galenos.2021.29488","url":null,"abstract":"<p><p>There are several blood-based markers to assess iron stores, but they all have some limitations. Hepcidin, a low-molecular-weight peptide hormone, is produced mainly by the liver. It is the main regulator of iron homeostasis by preventing iron release into plasma from absorptive enterocytes and macrophages. This review aims to critically assess existing data on potential role of hepcidin in diagnosis, particularly the (pre) analytical implications of the hepcidin measurement. There is a well-known causative correlation between hepcidin and iron deficiency. Therefore, hepcidin is considered as a promising marker in the assessment of iron status, particularly in patients with a diagnostic dilemma, such as patients with chronic renal disease and infants. The clinical implications of this peptide hormone in diagnosis of other diseases have been expanded in the recent studies, including elevated hepcidin levels in neoplastic diseases, sepsis, and inflammation. The potential role of hepcidin in diagnosis is controversial in the various types of iron deficiency because data are conflicting (as in anaemia of chronic disease) or limited (as in infants), whereas in the case of hereditary haemochromatosis, it has been proposed that hepcidin may be used for stratification of molecular testing, or to improve the frequency of phlebotomy, however, this issue still needs to be investigated. Due to lack of a clinically approved test, the medical application of this peptide as a biomarker in diagnosis is restricted. Recently, assays have been developed to determine hepcidin levels in serum and urine, facilitating the future use of hepcidin in research and clinical practice.</p>","PeriodicalId":23378,"journal":{"name":"Turkish Journal of Pharmaceutical Sciences","volume":"19 5","pages":"603-609"},"PeriodicalIF":1.7,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634449/pdf/TJPS-19-603.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40440132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
Turkish Journal of Pharmaceutical Sciences
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1