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Estimation of Hepcidin Role and some Biochemical Parameters in Patients with Beta- thalassemia in Thi-Qar Governorate/ Iraq 伊拉克济加尔省β-地中海贫血患者肝素作用和一些生化参数的估计
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1160
Alyaa Majid, S. Alyar, Mohammed Yasir Almusawi
The β-thalassemias are a group of recessively inherited genetic disorders that cause varying amounts of hemoglobin production. The study's aim was to look at hepcidin, ferretin, iron, PCV, Hb, ALT, AST, and albumin levels. Serum hepcidin, ferretin, iron, PCV, Hb, ALT, AST, and albumin levels were determined in 55 patients with beta-thalassemia and 55 healthy individuals. The results demonstrated a substantial rise in serum concentrations of ferretin, iron, ALT and AST in the beta-thalassemia group as compared to the control group(p<0.001).  There was also a significant decrease in the concentrations in the serum of hepcidin, packed cell volume, hemoglobin and albumin (p<0.001).  
β地中海贫血症是一组隐性遗传性疾病,会导致不同数量的血红蛋白生成。研究的目的是检测血红素、铁蛋白、铁、PCV、血红蛋白、谷丙转氨酶、谷草转氨酶和白蛋白水平。研究测定了 55 名地中海贫血患者和 55 名健康人的血清降血脂素、铁蛋白、铁、PCV、血红蛋白、谷丙转氨酶、谷草转氨酶和白蛋白水平。结果表明,与对照组相比,β-地中海贫血患者血清中铁素、铁、谷丙转氨酶和谷草转氨酶的浓度大幅上升(P<0.001)。 血红素、充盈细胞体积、血红蛋白和白蛋白的血清浓度也明显下降(P<0.001)。
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引用次数: 0
Test the effectiveness of some medications and vitamin D3 used against COVID-19 on Gram-positive and Gram-negative bacteria isolated from people infected with the Coronavirus 测试某些抗 COVID-19 的药物和维生素 D3 对从感染冠状病毒的人群中分离出的革兰氏阳性和革兰氏阴性细菌的效果
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1169
Amal F. Al-Gorani, Hanan Saadon, Ahmed. K. Ajeel, Noor Abd alkhudhur Salman, Aya Talib Jawad, Hussein Adnan Obaid, Hasan Mohsen Koti
The COVID-19 pandemic has led to a search for effective pharmaceuticals and vitamins to combat the virus. These therapies have antiviral capabilities and can affect both gram-negative and gram-positive bacteria. This study aims to assess the effectiveness of various medications and nutrients in treating bacterial infections linked to COVID-19. In vitro, tests will be conducted to evaluate these therapies' antibacterial efficacy against both gram-negative and gram-positive bacteria.In the current study, we test a group of antibiotics commonly used in treating Coronavirus on different types of Gram-positive and Gram-negative bacteria. They found that Azithromycin was highly effective against both positive and negative bacteria, but its effectiveness decreased when combined with vitamin D. Levofloxacin was the most effective antibiotic, and its efficacy was not affected by the addition of zinc or vitamin D. Acyclovir had a slight effect on bacteria when used alone, but its efficacy improved with zinc. Zinc and Vitamin D had a high degree of effectiveness, but Vitamin D had relatively low efficacy, except against one type of bacteria.The study aims to evaluate the effectiveness of drugs and vitamins against bacterial infections in COVID-19 patients. The results will provide a better understanding of the potential advantages of these medications in controlling bacterial infections. (The abstract should have one paragraph)The study aims to evaluate the effectiveness of drugs and vitamins against bacterial infections in COVID-19 patients. The results will provide a better understanding of the potential advantages of these medications in controlling bacterial infections.
COVID-19 大流行促使人们寻找有效的药物和维生素来对抗病毒。这些疗法具有抗病毒能力,并能影响革兰氏阴性菌和革兰氏阳性菌。这项研究旨在评估各种药物和营养素在治疗与 COVID-19 相关的细菌感染方面的有效性。我们将在体外进行测试,评估这些疗法对革兰氏阴性菌和革兰氏阳性菌的抗菌效果。在目前的研究中,我们测试了一组常用于治疗冠状病毒的抗生素对不同类型革兰氏阳性菌和革兰氏阴性菌的抗菌效果。他们发现,阿奇霉素对阳性和阴性细菌都非常有效,但与维生素 D 合用时,其疗效会下降。左氧氟沙星是最有效的抗生素,其疗效不受锌或维生素 D 的影响。锌和维生素 D 的疗效较高,但维生素 D 的疗效相对较低,只有对一种细菌的疗效除外。该研究旨在评估药物和维生素对 COVID-19 患者细菌感染的疗效。研究旨在评估药物和维生素对 COVID-19 患者细菌感染的疗效,研究结果将有助于更好地了解这些药物在控制细菌感染方面的潜在优势。(摘要应有一段话)该研究旨在评估药物和维生素对 COVID-19 患者细菌感染的疗效。研究结果将使人们更好地了解这些药物在控制细菌感染方面的潜在优势。
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引用次数: 0
Extraction and Identification of saponin extracts from Lepidium aucheri Boiss and antifungal properties evaluation 乌贼皂苷提取物的提取、鉴定及抗真菌特性评估
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1179
Mohammad Alkhuzaie, H. Kredy, Sajid. H. Guzar, Bassem Charfeddine, Ouahchi Ines
The analysis by High-performance liquid chromatography (HPLC) revealed that the saponin extracts contain a significant component called (Hederagenin glycoside). The wavelengths and electronic transitions of the active groups of the saponin extract were also determined using the UV-Vis Spectra. The optimal result was obtained by utilizing the Gemini C18 (5 µm) stationary phase manufactured by Phenomenex. They determine the optimal chromatographic conditions for enhancing LC/MS analysis by evaluating several mobile phases on a reversed-phase C18 column.  Illustrates the anticipated patterns of fragmentation observed in the mass spectra of substances. The Antifungal activity against Aspergillus flavus, Aspergillus Niger, and Aspergillus Alternaria was determined. The inhibitory effect in vitro was defined to appear, triterpenoid saponins extract showed maximum growth activity inhibitory of fungi against pathogens like Aspergillus Alternaria and Aspergillus Niger (2 cm), at the concentration (100 ppm) and the minimum growth activity inhibitory was against Aspergillus Alternaria (5 cm) at a concentration (25 ppm) compare with control. The triterpenoid saponins extract its ability to grow activity inhibition of fungi type Aspergillus Alternaria, Aspergillus Niger. On the other hand, the percentage of hemolysis activity in human blood increases with a high concentration of triterpenoid Saponins extract.
高效液相色谱法(HPLC)分析表明,皂苷提取物中含有一种名为 Hederagenin 苷的重要成分。此外,还利用紫外可见光谱测定了皂苷提取物活性基团的波长和电子跃迁。使用 Phenomenex 公司生产的 Gemini C18(5 µm)固定相取得了最佳结果。他们通过评估反相 C18 色谱柱上的几种流动相,确定了增强 LC/MS 分析的最佳色谱条件。 说明了在物质质谱中观察到的预期碎片模式。测定了对黄曲霉、尼日尔曲霉和交替曲霉的抗真菌活性。与对照组相比,三萜皂苷提取物在浓度(100 ppm)下对黄曲霉和尼日尔曲霉(2 厘米)等病原真菌的生长活性有最大的抑制作用,在浓度(25 ppm)下对黄曲霉(5 厘米)的生长活性抑制作用最小。三萜皂苷萃取物对交替曲霉、尼日尔曲霉等真菌的生长活性具有抑制作用。另一方面,三萜皂苷提取物浓度越高,人体血液中溶血活性的百分比也越高。
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引用次数: 0
The Effect of Input Pulse on the Soliton Generation in Microresonators 输入脉冲对微谐振器产生孤子的影响
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1158
Hawraa Alhassen, H. A. Sultan
The effect of the input pulse (chirped pulse) on the generation of soliton wave and optical frequency comb in microresonators was studied. The problem was solved numerically using the Lugiato-Lefever equation and the Fourier method by MATLAB program. The effect of Gaussian and ultra-Gaussian pulses, as well as chirped pulses, on the generation of the soliton wave and the frequency comb was also studied. Our study demonstrated that the generation of the soliton and the frequency comb depends on the shape of the pulse intensity distribution. Moreover, the mobility of the soliton and comb changes depending on the shape of the pulse. In addition, the results showed us that the soliton and frequency comb generated in microresonators are strongly affected by the power of the incoming pulse and the radius of the microresonator.
研究了输入脉冲(啁啾脉冲)对微谐振器中产生孤子波和光频率梳的影响。通过 MATLAB 程序,使用 Lugiato-Lefever 方程和傅里叶方法对该问题进行了数值求解。此外,还研究了高斯和超高斯脉冲以及啁啾脉冲对孤子波和频率梳产生的影响。我们的研究表明,孤子波和频率梳的产生取决于脉冲强度分布的形状。此外,孤子波和梳状波的迁移率也随脉冲形状的变化而变化。此外,研究结果还表明,微谐振器中产生的孤子和频率梳受输入脉冲的功率和微谐振器半径的影响很大。
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引用次数: 0
A Plasmonic Photonic Crystal Fiber Sensor with Simplified Features for Identifying Unidentified Analytes 用于识别不明分析物的具有简化功能的等离子体光子晶体光纤传感器
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1176
Hassan Yasser, Mostefa Benhaliliba
In this paper, we present a novel method for building a plasmonic photonic crystal fiber (PCF) sensor with simplified features to identify unknown analytes. Using the special parameters of plasmonic PCFs, the suggested sensor increases the sensitivity and specificity of target analyte detection. We provide a streamlined design approach that reduces manufacturing complexity without sacrificing reliable performance, opening up the sensor to new useful applications. We show the optimal structure parameters for optimizing sensitivity using the finite element method in the COMSOL environment. The suggested configuration attained strong spectral sensitivity throughout an extensive range of the analyte's refractive indices.  Furthermore, a perfect linear connection was established between the analyte's refractive index and the resonant wavelength, qualifying the sensor for usage across the whole range of the analyte's refractive indices.
在本文中,我们提出了一种构建具有简化功能的质子光子晶体光纤(PCF)传感器的新方法,以识别未知的分析物。利用质子光子晶体光纤的特殊参数,所建议的传感器提高了目标分析物检测的灵敏度和特异性。我们提供了一种简化的设计方法,在不牺牲可靠性能的前提下降低了制造复杂性,为传感器开辟了新的有用应用领域。我们在 COMSOL 环境中使用有限元法展示了优化灵敏度的最佳结构参数。所建议的结构在分析物折射率的广泛范围内都具有很强的光谱灵敏度。 此外,分析物折射率与谐振波长之间建立了完美的线性关系,使传感器在整个分析物折射率范围内都能使用。
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引用次数: 0
HFE Gene Mutations as Predisposing Factors for Childhood Acute Lymphoblastic Leukaemia in Iraqi Patients 伊拉克儿童急性淋巴细胞白血病的易感因素--HFE 基因突变
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1153
I. Albandar, Shilan Jabbar, Thanaa Kalil Ibrahim, Raghda Khalid
Hemochromatosis is a prevalent hereditary disorder that causes excess iron to build up in the body to dangerous levels. Hereditary hemochromatosis, also known as HFE-related hemochromatosis is carried on by changes in the HFE gene. Investigating the gene mutations of the HFE gene is a way to explore the prevalence of this disease. This study aims to determine the association between hemochromatosis HFE gene mutations (C282Y and H63D) and childhood acute lymphoblastic leukaemia in patients at Basra Specialized Hospital for Children and AL-sadder Teaching Hospital in the Basra governance. QIAamp DNA and Blood Mini Kit were used to isolate and identify Human genomic DNA and detect mutations in the HFE gene using the DNA hybridization method. In this study, the absence of the C282Y mutations in both patients and the control group was identified. However, testing DNA-based hybridization experiments revealed low detection levels of the H63D (homozygous, heterozygous) mutations; in only 12.5% of patients. The H63D (only homozygous) mutations were present in 10% of the control group. The association between patients and the control group is considered statistically significant. The HFE gene mutations (C282Y and H63D), originate in acute lymphoblastic leukaemia in childhood, thus, this study recommends complementary investigations to illustrate this case in more detail with more cases of patients and discover the hidden agents underlying these mutations.
血色沉着病是一种常见的遗传性疾病,会导致体内铁过量积聚,达到危险水平。遗传性血色病又称 HFE 相关性血色病,是由 HFE 基因的变化引起的。调查 HFE 基因的基因突变是探索这种疾病发病率的一种方法。本研究旨在确定巴士拉儿童专科医院(Basra Specialized Hospital for Children)和巴士拉AL-sadder教学医院(AL-sadder Teaching Hospital)患者的血色素沉着病HFE基因突变(C282Y和H63D)与儿童急性淋巴细胞白血病之间的关联。使用 QIAamp DNA 和血液迷你试剂盒分离和鉴定人类基因组 DNA,并使用 DNA 杂交法检测 HFE 基因突变。在这项研究中,发现患者和对照组均不存在 C282Y 突变。然而,基于 DNA 杂交实验的检测结果显示,H63D(同基因、杂合子)突变的检测水平较低;仅有 12.5%的患者检出了这种突变。对照组中有 10%的患者存在 H63D(仅同基因)突变。患者与对照组之间的关联被认为具有统计学意义。HFE 基因突变(C282Y 和 H63D)起源于儿童时期的急性淋巴细胞白血病,因此,本研究建议进行补充调查,以更多的患者病例更详细地说明这一病例,并发现这些突变背后隐藏的病原体。
{"title":"HFE Gene Mutations as Predisposing Factors for Childhood Acute Lymphoblastic Leukaemia in Iraqi Patients","authors":"I. Albandar, Shilan Jabbar, Thanaa Kalil Ibrahim, Raghda Khalid","doi":"10.32792/utq/utjsci/v11i1.1153","DOIUrl":"https://doi.org/10.32792/utq/utjsci/v11i1.1153","url":null,"abstract":"Hemochromatosis is a prevalent hereditary disorder that causes excess iron to build up in the body to dangerous levels. Hereditary hemochromatosis, also known as HFE-related hemochromatosis is carried on by changes in the HFE gene. Investigating the gene mutations of the HFE gene is a way to explore the prevalence of this disease. This study aims to determine the association between hemochromatosis HFE gene mutations (C282Y and H63D) and childhood acute lymphoblastic leukaemia in patients at Basra Specialized Hospital for Children and AL-sadder Teaching Hospital in the Basra governance. QIAamp DNA and Blood Mini Kit were used to isolate and identify Human genomic DNA and detect mutations in the HFE gene using the DNA hybridization method. In this study, the absence of the C282Y mutations in both patients and the control group was identified. However, testing DNA-based hybridization experiments revealed low detection levels of the H63D (homozygous, heterozygous) mutations; in only 12.5% of patients. The H63D (only homozygous) mutations were present in 10% of the control group. The association between patients and the control group is considered statistically significant. The HFE gene mutations (C282Y and H63D), originate in acute lymphoblastic leukaemia in childhood, thus, this study recommends complementary investigations to illustrate this case in more detail with more cases of patients and discover the hidden agents underlying these mutations.","PeriodicalId":23432,"journal":{"name":"University of Thi-Qar Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141275091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Susceptibility in a Coupled Double Quantum Dot-Metal Nanoparticle System under Standing Wave Field 驻波场下双量子点-金属纳米粒子耦合系统中的电感应强度
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1156
Haneen Akram, Muwaffaq Abdullah, El Mustapha Feddi, Amin H. Al-Khursan, Ali M. Muslim
The main purpose of this work is studying the linear Susceptibility in the hybrid nanostructure that composed of a dual quantum dot (DQD) and metal nanoparticle (MNP) hybrid system under a standing-wave field. In our model, we used density matrix equations by taking into our account the interaction between excitons and surface plasmons. The proposed DQD is composed of two QDs. Each QD contains an InAs QD with a disk shape. The interaction between the QD and the wetting layer (WL) is taken into consideration. The application of the standing wave field on DQD-MNP hybrid system was modeled and examined. The susceptibility of thehybridDQD-MNPsystem reduced by the pump field under a standing-wave field. The high susceptibility obtained with a wide MNP radius. An interesting result was shown in the inversion of the grating period with the tunneling component in the conduction band. The smaller size of DQD gave us high susceptibility due to the quantization effect.
这项工作的主要目的是研究由双量子点(DQD)和金属纳米粒子(MNP)混合系统组成的混合纳米结构在驻波场下的线性易感性。在我们的模型中,我们使用了密度矩阵方程,考虑了激子和表面质子之间的相互作用。拟议的 DQD 由两个 QD 组成。每个 QD 包含一个圆盘形状的 InAs QD。我们考虑了 QD 与润湿层 (WL) 之间的相互作用。对驻波场在 DQD-MNP 混合系统上的应用进行了建模和检验。在驻波场下,泵场降低了 DQD-MNP 混合系统的电感。MNP 半径越大,电感率越高。一个有趣的结果显示了光栅周期与导带中隧道分量的反转。由于量子化效应,较小尺寸的 DQD 具有较高的电感。
{"title":"Susceptibility in a Coupled Double Quantum Dot-Metal Nanoparticle System under Standing Wave Field","authors":"Haneen Akram, Muwaffaq Abdullah, El Mustapha Feddi, Amin H. Al-Khursan, Ali M. Muslim","doi":"10.32792/utq/utjsci/v11i1.1156","DOIUrl":"https://doi.org/10.32792/utq/utjsci/v11i1.1156","url":null,"abstract":"The main purpose of this work is studying the linear Susceptibility in the hybrid nanostructure that composed of a dual quantum dot (DQD) and metal nanoparticle (MNP) hybrid system under a standing-wave field. In our model, we used density matrix equations by taking into our account the interaction between excitons and surface plasmons. The proposed DQD is composed of two QDs. Each QD contains an InAs QD with a disk shape. The interaction between the QD and the wetting layer (WL) is taken into consideration. The application of the standing wave field on DQD-MNP hybrid system was modeled and examined. The susceptibility of thehybridDQD-MNPsystem reduced by the pump field under a standing-wave field. The high susceptibility obtained with a wide MNP radius. An interesting result was shown in the inversion of the grating period with the tunneling component in the conduction band. The smaller size of DQD gave us high susceptibility due to the quantization effect.","PeriodicalId":23432,"journal":{"name":"University of Thi-Qar Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141231050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of Saturable Absorber Length Effect on Characteristics of Passive Q-Switching and Stokes with Anti-Stokes Pulses Generated in Laser System of Nd:YVO4 研究可饱和吸收体长度对 Nd:YVO4 激光系统中产生的无源 Q 开关和斯托克斯与反斯托克斯脉冲特性的影响
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1146
Dunya Saad, Abdul-Kareem Mahdi Salih, Rasool Asal
The effect of  the saturable  absorbent material on the characteristics of a single laser pulse results from the passive Q-switching method, or two pulses. The first pulse is resulted from the passive Q-switching method and the other is resulted from Stokes pulse generated by the laser ray reaction with the Raman medium in the optical system. This study simulated this effect on the characteristics of three pulses (passive Q-switching  pulse, Stokes pulse, and anti-Stokes pulse) simultaneously generated from one optical system  . The optical system consists of Nd: YVO4, PbWO4, and Cr + 4: YAG as an effective medium, Raman medium, and saturable absorbent material, respectively. The modeling of the rate equations was used, and the Rung-Kutta-Fehelberg numerical method was used to solve the rate equations. The results show increased power of the pulses generated, requiring an increased length of the saturable absorber material used in the system. The results showed that at the shortest length used for the saturated absorbent material, the pulse duration and energy of the three generated pulses were PQS pulse (16.44ns, 1.39mJ), Stokes pulse  (12.59ns, 1.148mJ) and anti-Stokes pulse (13.53ns, 1.59mJ), while at the longest length of the saturated absorbent material, the pulse duration and energy were PQS pulse (14.68ns, 1.50mJ), the Stokes pulse (11.41ns, 1.36mJ) and the anti-Stokes pulse (12.38ns, 1.86mJ).
可饱和吸收材料对单个激光脉冲特性的影响来自于被动 Q 开关方法或两个脉冲。第一个脉冲由被动 Q 开关法产生,另一个脉冲由激光射线与光学系统中的拉曼介质反应产生的斯托克斯脉冲产生。本研究模拟了这种效应对一个光学系统同时产生的三个脉冲(被动 Q 开关脉冲、斯托克斯脉冲和反斯托克斯脉冲)特性的影响。光学系统由 Nd:YVO4、PbWO4 和 Cr + 4:YAG 组成,分别作为有效介质、拉曼介质和可饱和吸收材料。采用速率方程建模,并使用 Rung-Kutta-Fehelberg 数值方法求解速率方程。结果表明,产生的脉冲功率增大,要求系统中使用的可饱和吸收材料的长度增加。结果表明,在饱和吸收材料使用的最短长度下,产生的三个脉冲的脉冲持续时间和能量分别为 PQS 脉冲(16.44ns,1.39mJ)、斯托克斯脉冲(12.59ns,1.148mJ)和反斯托克斯脉冲(13.53ns,1.59mJ)。53ns,1.59mJ),而在饱和吸收材料的最长长度上,脉冲持续时间和能量分别为 PQS 脉冲(14.68ns,1.50mJ)、斯托克斯脉冲(11.41ns,1.36mJ)和反斯托克斯脉冲(12.38ns,1.86mJ)。
{"title":"Investigation of Saturable Absorber Length Effect on Characteristics of Passive Q-Switching and Stokes with Anti-Stokes Pulses Generated in Laser System of Nd:YVO4","authors":"Dunya Saad, Abdul-Kareem Mahdi Salih, Rasool Asal","doi":"10.32792/utq/utjsci/v11i1.1146","DOIUrl":"https://doi.org/10.32792/utq/utjsci/v11i1.1146","url":null,"abstract":"The effect of  the saturable  absorbent material on the characteristics of a single laser pulse results from the passive Q-switching method, or two pulses. The first pulse is resulted from the passive Q-switching method and the other is resulted from Stokes pulse generated by the laser ray reaction with the Raman medium in the optical system. This study simulated this effect on the characteristics of three pulses (passive Q-switching  pulse, Stokes pulse, and anti-Stokes pulse) simultaneously generated from one optical system  . The optical system consists of Nd: YVO4, PbWO4, and Cr + 4: YAG as an effective medium, Raman medium, and saturable absorbent material, respectively. The modeling of the rate equations was used, and the Rung-Kutta-Fehelberg numerical method was used to solve the rate equations. The results show increased power of the pulses generated, requiring an increased length of the saturable absorber material used in the system. The results showed that at the shortest length used for the saturated absorbent material, the pulse duration and energy of the three generated pulses were PQS pulse (16.44ns, 1.39mJ), Stokes pulse  (12.59ns, 1.148mJ) and anti-Stokes pulse (13.53ns, 1.59mJ), while at the longest length of the saturated absorbent material, the pulse duration and energy were PQS pulse (14.68ns, 1.50mJ), the Stokes pulse (11.41ns, 1.36mJ) and the anti-Stokes pulse (12.38ns, 1.86mJ).","PeriodicalId":23432,"journal":{"name":"University of Thi-Qar Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141233997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sequencing of ribosome-based amplicons in five isolates Dipodascus Capitus from patients with sinusitis 对从鼻窦炎患者中分离出的五种 Dipodascus Capitus 核糖体扩增子进行测序
Pub Date : 2024-06-01 DOI: 10.32792/utq/utjsci/v11i1.1172
Iman Hadi, Ahmed Abdel-Azeem
Sequencing reactions showed the accurate identity of the investigated samples and revealed that all investigated samples were homologous to Dipodascus capitatus (GenBank acc. no. OW983902.1). The presence of one nucleic acid variant compared with the referring sequences of the D. capitatussequences was demonstrated. The identified variant was represented by a nucleic acid substitution (359A>T) detected in the S5 sample. Meanwhile, the rest of the samples showed a complete homology with the corresponding sequences and did not exhibit any detectable nucleic acid variations in comparison with the D. capitatus reference sequences. It was inferred from the tree that the detected nucleic acid substitutions showed a slight effect of the observed variations on the altering evolutionary positioning of the investigated S5 sample in comparison with the other wild-type sequences. This was due to the positioning of the altered samples in the other positions compared with the wild-type counterparts. It was inferred from the tree that our investigated D. capitatus samples were suited in the immediate vicinity to various strains deposited from various multi-national positions. Furthermore, neighbor phylogenetic distances in this tree indicated a distinct biological diversity of D. capitatus sequences.
测序反应显示了所调查样本的准确身份,并揭示所有调查样本都与岬蛙 Dipodascus capitatus(GenBank 编号:OW983902.1)同源。与 D. capitatus 序列的参考序列相比,发现了一个核酸变体。在 S5 样品中检测到的核酸替换(359A>T)代表了该变异。与此同时,其他样本与相应的序列显示出完全的同源性,与蝙蝠的参考序列相比没有显示出任何可检测到的核酸变异。从树状图中可以推断,与其他野生型序列相比,检测到的核酸置换显示出观察到的变异对被调查的 S5 样本进化定位的改变有轻微影响。这是由于与野生型样本相比,变异样本在其他位置的定位发生了变化。从该树中可以推断出,我们所研究的蝙蝠蛾样本与来自不同国家的各种菌株相邻。此外,这棵树中的邻近系统发育距离表明绒毛膜促性腺激素序列具有独特的生物多样性。
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引用次数: 0
Investigation of Antimicrobial Susceptibility Patterns and blaVIM -metallo-β-lactamase Gene in Clinical Samples of Klebsiella pneumoniae 肺炎克雷伯菌临床样本的抗菌药敏感性模式和 blaVIM -金属-β-内酰胺酶基因研究
Pub Date : 2023-12-30 DOI: 10.32792/utq/utjsci/v10i2.1149
Asaad Alkhafaji, Neda Soleimani, Hind Mousa
Multidrug resistance is a widespread issue that plays an important role in disease outcome. This study was designed to isolate Klebsiella pneumonia in different clinical specimens and detected their Antibiotic resistance profile. A total of 319 samples were collected from various clinical specimens for both genders and different ages. The samples were streaked on the blood and MacConkey agars.  The bacterial growth identified using biochemical tests and Vitek®2 systems to confirm it. Also, the Vitek®2 system was used to detect the antibiotic sensitivity. Out of 319 clinical samples, K. pneumonia was identified in 67 (21%) cases. The highest isolation rate was in urine 25(37.3%), followed by sputum 13(19.4%), and the least isolation was in CSF with one isolate (1.5%). The results revealed that K. pneumonia isolates were multi-drug resistant pathogens (MDR) with high resistance to Ampicillin (97%) and 85% for piperacillin. The PCR results revealed blaVIM- genes frequency was 20 (30%). K. pneumonia is one of the bacteria that cause urinary tract infections, and it is a widespread multidrug-resistant pathogen, and blaVIM- producing K. pneumoniae are found in clinical samples at Thi-Qar hospitals. Therefore, monitoring the administration of antibiotics and their rational use is necessary to reduce antimicrobial resistance and treatment failure.
多重耐药性是一个普遍存在的问题,对疾病的治疗结果起着重要作用。本研究旨在分离不同临床标本中的肺炎克雷伯氏菌,并检测其抗生素耐药性情况。研究人员从不同的临床标本中收集了 319 份样本,这些样本来自不同的性别和年龄。这些样本分别在血琼脂和麦康凯琼脂上做条痕培养。 使用生化测试和 Vitek®2 系统对细菌生长情况进行确认。此外,Vitek®2 系统还用于检测抗生素敏感性。在 319 份临床样本中,有 67 例(21%)鉴定出肺炎克氏菌。分离率最高的是尿液,有 25 例(37.3%),其次是痰液,有 13 例(19.4%),分离率最低的是脑脊液,有 1 例(1.5%)。结果显示,肺炎克雷伯菌属多重耐药病原体(MDR),对氨苄西林耐药率高(97%),对哌拉西林耐药率高(85%)。PCR 结果显示 blaVIM- 基因频率为 20(30%)。肺炎双球菌是导致尿路感染的细菌之一,是一种广泛存在的多重耐药病原体,在济喀医院的临床样本中发现了产生 blaVIM- 的肺炎双球菌。因此,有必要监测抗生素的使用情况并合理使用抗生素,以减少抗生素耐药性和治疗失败。
{"title":"Investigation of Antimicrobial Susceptibility Patterns and blaVIM -metallo-β-lactamase Gene in Clinical Samples of Klebsiella pneumoniae","authors":"Asaad Alkhafaji, Neda Soleimani, Hind Mousa","doi":"10.32792/utq/utjsci/v10i2.1149","DOIUrl":"https://doi.org/10.32792/utq/utjsci/v10i2.1149","url":null,"abstract":"Multidrug resistance is a widespread issue that plays an important role in disease outcome. This study was designed to isolate Klebsiella pneumonia in different clinical specimens and detected their Antibiotic resistance profile. A total of 319 samples were collected from various clinical specimens for both genders and different ages. The samples were streaked on the blood and MacConkey agars.  The bacterial growth identified using biochemical tests and Vitek®2 systems to confirm it. Also, the Vitek®2 system was used to detect the antibiotic sensitivity. Out of 319 clinical samples, K. pneumonia was identified in 67 (21%) cases. The highest isolation rate was in urine 25(37.3%), followed by sputum 13(19.4%), and the least isolation was in CSF with one isolate (1.5%). The results revealed that K. pneumonia isolates were multi-drug resistant pathogens (MDR) with high resistance to Ampicillin (97%) and 85% for piperacillin. The PCR results revealed blaVIM- genes frequency was 20 (30%). K. pneumonia is one of the bacteria that cause urinary tract infections, and it is a widespread multidrug-resistant pathogen, and blaVIM- producing K. pneumoniae are found in clinical samples at Thi-Qar hospitals. Therefore, monitoring the administration of antibiotics and their rational use is necessary to reduce antimicrobial resistance and treatment failure.","PeriodicalId":23432,"journal":{"name":"University of Thi-Qar Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139136785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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University of Thi-Qar Journal of Science
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